LOCUS       NM_023932               1548 bp    mRNA    linear   PRI 01-MAR-2025
DEFINITION  Homo sapiens delta like non-canonical Notch ligand 2 (DLK2),
            transcript variant 1, mRNA.
ACCESSION   NM_023932
VERSION     NM_023932.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Naranjo,A.I., Gonzalez-Gomez,M.J., Baladron,V., Laborda,J. and
            Nueda,M.L.
  TITLE     Different Expression Levels of DLK2 Inhibit NOTCH Signaling and
            Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo
  JOURNAL   Int J Mol Sci 23 (3), 1554 (2022)
   PUBMED   35163478
  REMARK    GeneRIF: Different Expression Levels of DLK2 Inhibit NOTCH
            Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor
            Growth In Vivo.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Meng,F., Wu,L., Dong,L., Mitchell,A.V., James Block,C., Liu,J.,
            Zhang,H., Lu,Q., Song,W.M., Zhang,B., Chen,W., Hu,J., Wang,J.,
            Yang,Q., Huttemann,M. and Wu,G.
  TITLE     EGFL9 promotes breast cancer metastasis by inducing cMET activation
            and metabolic reprogramming
  JOURNAL   Nat Commun 10 (1), 5033 (2019)
   PUBMED   31695034
  REMARK    GeneRIF: EGFL9 promotes breast cancer metastasis by inducing cMET
            activation and metabolic reprogramming.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1548)
  AUTHORS   Nueda,M.L., Naranjo,A.I., Baladron,V. and Laborda,J.
  TITLE     The proteins DLK1 and DLK2 modulate NOTCH1-dependent proliferation
            and oncogenic potential of human SK-MEL-2 melanoma cells
  JOURNAL   Biochim Biophys Acta 1843 (11), 2674-2684 (2014)
   PUBMED   25093684
REFERENCE   4  (bases 1 to 1548)
  AUTHORS   Nueda,M.L., Baladron,V., Garcia-Ramirez,J.J., Sanchez-Solana,B.,
            Ruvira,M.D., Rivero,S., Ballesteros,M.A., Monsalve,E.M.,
            Diaz-Guerra,M.J., Ruiz-Hidalgo,M.J. and Laborda,J.
  TITLE     The novel gene EGFL9/Dlk2, highly homologous to Dlk1, functions as
            a modulator of adipogenesis
  JOURNAL   J Mol Biol 367 (5), 1270-1280 (2007)
   PUBMED   17320102
  REMARK    GeneRIF: Studies in mouse showed that dlk2, highly homologous to
            dlk1 (also known as pref-1, and FA-1) appears to modulate
            adipogenesis in vitro in an opposite way to that of dlk1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC110320.1.
            
            On Nov 23, 2018 this sequence version replaced NM_023932.3.
            
            Transcript Variant: This variant (1) differs in the 5' UTR,
            compared to variant 2. Both variants 1 and 2 encode the same
            isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110320.1, BE790917.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2150585
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372488.8/ ENSP00000361566.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1548              BC110320.1         45-1592
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..1548
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /note="delta like non-canonical Notch ligand 2"
                     /db_xref="GeneID:65989"
                     /db_xref="HGNC:HGNC:21113"
                     /db_xref="MIM:621120"
     exon            1..154
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     exon            155..285
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..1361
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; EGF-like-domain, multiple 9; protein delta
                     homolog 2; EGF-like protein 9; epidermal growth
                     factor-like protein 9; delta-like 2 homolog"
                     /codon_start=1
                     /product="protein delta homolog 2 isoform a precursor"
                     /protein_id="NP_076421.2"
                     /db_xref="CCDS:CCDS4897.1"
                     /db_xref="GeneID:65989"
                     /db_xref="HGNC:HGNC:21113"
                     /db_xref="MIM:621120"
                     /translation="MPSGCRCLHLVCLLCILGAPGQPVRADDCSSHCDLAHGCCAPDG
                     SCRCDPGWEGLHCERCVRMPGCQHGTCHQPWQCICHSGWAGKFCDKDEHICTTQSPCQ
                     NGGQCMYDGGGEYHCVCLPGFHGRDCERKAGPCEQAGSPCRNGGQCQDDQGFALNFTC
                     RCLVGFVGARCEVNVDDCLMRPCANGATCLDGINRFSCLCPEGFAGRFCTINLDDCAS
                     RPCQRGARCRDRVHDFDCLCPSGYGGKTCELVLPVPDPPTTVDTPLGPTSAVVVPATG
                     PAPHSAGAGLLRISVKEVVRRQEAGLGEPSLVALVVFGALTAALVLATVLLTLRAWRR
                     GVCPPGPCCYPAPHYAPACQDQECQVSMLPAGLPLPRDLPPEPGKTTAL"
     exon            286..349
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     exon            350..480
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     exon            481..625
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     exon            626..1548
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1528..1533
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /note="hexamer: AATAAA"
     polyA_site      1548
                     /gene="DLK2"
                     /gene_synonym="DLK-2; EGFL9"
                     /note="major polyA site"
ORIGIN      
        1 agattcccga gcgctcggct cgcatggcag ccgcttcggc gcccggcccc gcggccagct
       61 aggggcggcc ccgcgctccc tcacggcccc tcggcggcgc ccgtcggatc cggcctctct
      121 ctgcgccccg gggcgcgcca cctccccgcc ggaggtgtcc acgcgtccgg ccgtccatcc
      181 gtccgtccct cctggggccg gcgctgacca tgcccagcgg ctgccgctgc ctgcatctcg
      241 tgtgcctgtt gtgcattctg ggggctcccg gtcagcctgt ccgagccgat gactgcagct
      301 cccactgtga cctggcccac ggctgctgtg cacctgacgg ctcctgcagg tgtgacccgg
      361 gctgggaggg gctgcactgt gagcgctgtg tgaggatgcc tggctgccag cacggtacct
      421 gccaccagcc atggcagtgc atctgccaca gtggctgggc aggcaagttc tgtgacaaag
      481 atgaacatat ctgtaccacg cagtccccct gccagaatgg aggccagtgc atgtatgacg
      541 ggggcggtga gtaccattgt gtgtgcttac caggcttcca tgggcgtgac tgcgagcgca
      601 aggctggacc ctgtgaacag gcaggctccc catgccgcaa tggcgggcag tgccaggacg
      661 accagggctt tgctctcaac ttcacgtgcc gctgcttggt gggctttgtg ggtgcccgct
      721 gtgaggtaaa tgtggatgac tgcctgatgc ggccttgtgc taacggtgcc acctgccttg
      781 acggcataaa ccgcttctcc tgcctctgtc ctgagggctt tgctggacgc ttctgcacca
      841 tcaacctgga tgactgtgcc agccgcccat gccagagagg ggcccgctgt cgggaccgtg
      901 tccacgactt cgactgcctc tgccccagtg gctatggtgg caagacctgt gagcttgtct
      961 tacctgtccc agacccccca accacagtgg acacccctct agggcccacc tcagctgtag
     1021 tggtacctgc cacggggcca gccccccaca gcgcaggggc tggtctgctg cggatctcag
     1081 tgaaggaggt ggtgcggagg caagaggctg ggctaggtga gcctagcttg gtggccctgg
     1141 tggtgtttgg ggccctcact gctgccctgg ttctggctac tgtgttgctg accctgaggg
     1201 cctggcgccg gggtgtctgc ccccctggac cctgttgcta ccctgcccca cactatgctc
     1261 cagcgtgcca ggaccaggag tgtcaggtta gcatgctgcc agcagggctc cccctgccac
     1321 gtgacttgcc ccctgagcct ggaaagacca cagcactgtg atggaggtgg gggctttctg
     1381 gcccccttcc tcacctcttc cacccctcag actggagtgg tccgttctca ccacccttca
     1441 gcttgggtac acacacagag gagacctcag cctcacacca gaaatattat ttttttaata
     1501 cacagaatgt aagatggaat tttatcaaat aaaactatga aaatgcaa
//
