LOCUS       NM_007335               6899 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DLEC1 cilia and flagella associated protein (DLEC1),
            transcript variant DLEC1-N1, mRNA.
ACCESSION   NM_007335 NM_005106
VERSION     NM_007335.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Chou,Y.H., Tantoh,D.M., Wu,M.C., Tyan,Y.S., Chen,P.H., Nfor,O.N.,
            Hsu,S.Y., Shen,C.Y., Huang,C.N. and Liaw,Y.P.
  TITLE     PM2.5 exposure and DLEC1 promoter methylation in Taiwan Biobank
            participants
  JOURNAL   Environ Health Prev Med 25 (1), 68 (2020)
   PUBMED   33153431
  REMARK    GeneRIF: PM2.5 exposure and DLEC1 promoter methylation in Taiwan
            Biobank participants.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Okitsu,Y., Nagano,M., Yamagata,T., Ito,C., Toshimori,K., Dohra,H.,
            Fujii,W. and Yogo,K.
  TITLE     Dlec1 is required for spermatogenesis and male fertility in mice
  JOURNAL   Sci Rep 10 (1), 18883 (2020)
   PUBMED   33144677
  REMARK    GeneRIF: Dlec1 is required for spermatogenesis and male fertility
            in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6899)
  AUTHORS   Xie,W., Zhou,H., Han,Q., Sun,T., Nie,C., Hong,J., Wei,R.,
            Leonteva,A., Han,X., Wang,J., Du,X., Zhu,L., Zhao,Y., Tian,W. and
            Xue,Y.
  TITLE     Relationship between DLEC1 and PBX3 promoter methylation and the
            risk and prognosis of gastric cancer in peripheral blood leukocytes
  JOURNAL   J Cancer Res Clin Oncol 146 (5), 1115-1124 (2020)
   PUBMED   32144534
  REMARK    GeneRIF: Compared with negative methylation (Nm), DLEC1-positive
            methylation (Pm) was associated with increased GC risk in PS (OR
            2.083, 95% CI 1.220-3.558, P = 0.007), but PBX3 Pm was not
            associated with GC risk.
REFERENCE   4  (bases 1 to 6899)
  AUTHORS   Brany,D., Dvorska,D., Grendar,M., Nachajova,M., Szepe,P.,
            Lasabova,Z., Zubor,P., Visnovsky,J. and Halasova,E.
  TITLE     Different methylation levels in the KLF4, ATF3 and DLEC1 genes in
            the myometrium and in corpus uteri mesenchymal tumours as assessed
            by MS-HRM
  JOURNAL   Pathol Res Pract 215 (8), 152465 (2019)
   PUBMED   31176573
  REMARK    GeneRIF: that the KLF4 and DLEC1 genes can be considered potential
            methylation biomarkers for uterine leiomyomas
REFERENCE   5  (bases 1 to 6899)
  AUTHORS   Kim,Y., Lee,K., Jeong,S., Wen,X., Cho,N.Y. and Kang,G.H.
  TITLE     DLEC1 methylation is associated with a better clinical outcome in
            patients with intrahepatic cholangiocarcinoma of the small duct
            subtype
  JOURNAL   Virchows Arch 475 (1), 49-58 (2019)
   PUBMED   30610381
  REMARK    GeneRIF: DLEC1 methylation can be utilized to identify a subset
            with a better prognosis in intrahepatic cholangiocarcinomas of the
            small duct type.
REFERENCE   6  (bases 1 to 6899)
  AUTHORS   Seng,T.J., Currey,N., Cooper,W.A., Lee,C.S., Chan,C., Horvath,L.,
            Sutherland,R.L., Kennedy,C., McCaughan,B. and Kohonen-Corish,M.R.
  TITLE     DLEC1 and MLH1 promoter methylation are associated with poor
            prognosis in non-small cell lung carcinoma
  JOURNAL   Br J Cancer 99 (2), 375-382 (2008)
   PUBMED   18594535
  REMARK    GeneRIF: DLEC1 methylation was an independent marker of poor
            survival in patients with non-small cell lung carcinoma.
REFERENCE   7  (bases 1 to 6899)
  AUTHORS   Qiu,G.H., Salto-Tellez,M., Ross,J.A., Yeo,W., Cui,Y.,
            Wheelhouse,N., Chen,G.G., Harrison,D., Lai,P., Tao,Q. and Hooi,S.C.
  TITLE     The tumor suppressor gene DLEC1 is frequently silenced by DNA
            methylation in hepatocellular carcinoma and induces G1 arrest in
            cell cycle
  JOURNAL   J Hepatol 48 (3), 433-441 (2008)
   PUBMED   18191269
  REMARK    GeneRIF: DLEC1 is a candidate tumor suppressor gene that plays an
            important role in the development and progression of hepatocellular
            carcinoma.
REFERENCE   8  (bases 1 to 6899)
  AUTHORS   Kwong,J., Chow,L.S., Wong,A.Y., Hung,W.K., Chung,G.T., To,K.F.,
            Chan,F.L., Daigo,Y., Nakamura,Y., Huang,D.P. and Lo,K.W.
  TITLE     Epigenetic inactivation of the deleted in lung and esophageal
            cancer 1 gene in nasopharyngeal carcinoma
  JOURNAL   Genes Chromosomes Cancer 46 (2), 171-180 (2007)
   PUBMED   17099870
  REMARK    GeneRIF: Silencing of DLEC1 expression by promoter hypermethylation
            and histone deacetylation may be important in nasopharyngeal
            carcinoma tumorigenesis.
REFERENCE   9  (bases 1 to 6899)
  AUTHORS   Kwong,J., Lee,J.Y., Wong,K.K., Zhou,X., Wong,D.T., Lo,K.W.,
            Welch,W.R., Berkowitz,R.S. and Mok,S.C.
  TITLE     Candidate tumor-suppressor gene DLEC1 is frequently downregulated
            by promoter hypermethylation and histone hypoacetylation in human
            epithelial ovarian cancer
  JOURNAL   Neoplasia 8 (4), 268-278 (2006)
   PUBMED   16756719
  REMARK    GeneRIF: DLEC1 suppresses the growth of ovarian cancer cells and
            its downregulation is closely associated with promoter
            hypermethylation and histone hypoacetylation
REFERENCE   10 (bases 1 to 6899)
  AUTHORS   Daigo,Y., Nishiwaki,T., Kawasoe,T., Tamari,M., Tsuchiya,E. and
            Nakamura,Y.
  TITLE     Molecular cloning of a candidate tumor suppressor gene, DLC1, from
            chromosome 3p21.3
  JOURNAL   Cancer Res 59 (8), 1966-1972 (1999)
   PUBMED   10213508
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB020522.1, AL137706.1,
            CB243860.1 and AP006309.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_007335.3.
            
            Summary: The cytogenetic location of this gene is 3p21.3, and it is
            located in a region that is commonly deleted in a variety of
            malignancies. Down-regulation of this gene has been observed in
            several human cancers including lung, esophageal, renal tumors, and
            head and neck squamous cell carcinoma. In some cases, reduced
            expression of this gene in tumor cells is a result of aberrant
            promoter methylation. Several alternatively spliced transcripts
            have been observed that contain disrupted coding regions and likely
            encode nonfunctional proteins.[provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (DLEC1-N1) encodes the shortest
            isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB020522.1, BC171799.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308059.11/ ENSP00000308597.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4341              AB020522.1         4-4344
            4342-5606           AL137706.1         3227-4491
            5607-5615           CB243860.1         18-26               c
            5616-6899           AP006309.1         103222-104505
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..6899
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="DLEC1 cilia and flagella associated protein"
                     /db_xref="GeneID:9940"
                     /db_xref="HGNC:HGNC:2899"
                     /db_xref="MIM:604050"
     exon            1..429
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..5286
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="isoform DLEC1-N1 is encoded by transcript variant
                     DLEC1-N1; cilia and flagella associated protein 81; DLEC,
                     cilia and flagella associated protein; deleted in lung and
                     esophageal cancer 1"
                     /codon_start=1
                     /product="deleted in lung and esophageal cancer protein 1
                     isoform DLEC1-N1"
                     /protein_id="NP_031361.2"
                     /db_xref="CCDS:CCDS2672.2"
                     /db_xref="GeneID:9940"
                     /db_xref="HGNC:HGNC:2899"
                     /db_xref="MIM:604050"
                     /translation="METRSSKTRRSLASRTNECQGTMWAPTSPPAGSSSPSQPTWKSS
                     LYSSLAYSEAFHYSFAARPRRLTQLALAQRPEPQLLRLRPSSLRTQDISHLLTGVFRN
                     LYSAEVIGDEVSASLIKARGSENERHEEFVDQLQQIRELYKQRLDEFEMLERHITQAQ
                     ARAIAENERVMSQAGVQDLESLVRLPPVKSVSRWCIDSELLRKHHLISPEDYYTDTVP
                     FHSAPKGISLPGCSKLTFSCEKRSVQKKELNKKLEDSCRKKLAEFEDELDHTVDSLTW
                     NLTPKAKERTREPLKKASQPRNKNWMNHLRVPQRELDRLLLARMESRNHFLKNPRFFP
                     PNTRYGGKSLVFPPKKPAPIGEFQSTEPEQSCADTPVFLAKPPIGFFTDYEIGPVYEM
                     VIALQNTTTTSRYLRVLPPSTPYFALGLGMFPGKGGMVAPGMTCQYIVQFFPDCLGDF
                     DDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGVKMTRFICKNVGFSVG
                     RFCIMPKTSWPPLSFKAIATVGFVEQPPFGILPSVFELAPGHAILVEVLFSPKSLGKA
                     EQTFIIMCDNCQIKELVTIGIGQLIALDLIYISGEKSQPDPGELTDLTAQHFIRFEPE
                     NLRSTARKQLIIRNATHVELAFYWQIMKPNLQPLMPGETFSMDSIKCYPDKETAFSIM
                     PRKGVLSPHTDHEFILSFSPHELRDFHSVLQMVLEEVPEPVSSEAESLGHSSYSVDDV
                     IVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKAFKMWNNSKSPIRYLWGKISD
                     CHIIEVEPGTGVIEPSEVGDFELNFTGGVPGPTSQDLLCEIEDSPSPVVLHIEAVFKG
                     PALIINVSALQFGLLRLGQKATNSIQIRNVSQLPATWRMKESPVSLQERPEDVSPFDI
                     EPSSGQLHSLGECRVDITLEALHCQHLETVLELEVENGAWSYLPVYAEVQKPHVYLQS
                     SQVEVRNLYLGVPTKTTITLINGTLLPTQFHWGKLLGHQAEFCMVTVSPKHGLLGPSE
                     ECQLKLELTAHTQEELTHLALPCHVSGMKKPLVLGISGKPQGLQVAITISKESSDCST
                     EQWPGHPKELRLDFGSAVPLRTRVTRQLILTNRSPIRTRFSLKFEYFGSPQNSLSKKT
                     SLPNMPPALLKTVRMQEHLAKREQLDFMESMLSHGKGAAFFPHFSQGMLGPYQQLCID
                     ITGCANMWGEYWDNLICTVGDLLPEVIPVHMAAVGCPISSLRTTSYTIDQAQKEPAMR
                     FGTQVSGGDTVTRTLRLNNSSPCDIRLDWETYVPEDKEDRLVELLVFYGPPFPLRDQA
                     GNELVCPDTPEGGCLLWSPGPSSSSEFSHETDSSVEGSSSASNRVAQKLISVILQAHE
                     GVPSGHLYCISPKQVVVPAGGSSTIYISFTPMVLSPEILHKVECTGYALGFMSLDSKV
                     EREIPGKRHRLQDFAVGPLKLDLHSYVRPAQLSVELDYGGSMEFQCQASDLIPEQPCS
                     GVLSELVTTHHLKLTNTTEIPHYFRLMVSRPFSVSQDGASQDHRAPGPGQKQECEEET
                     ASADKQLVLQAQENMLVNVSFSLSLELLSYQKLPADQTLPGVDIQQSASGEREMVFTQ
                     NLLLEYTNQTTQVVPLRAVVAVPELQLSTSWVDFGTCFVSQQRVREVYLMNLSGCRSY
                     WTMLMGQQEPAKAAVAFRVSPNSGLLEARSANAPPTSIALQVFFTARSSELYESTMVV
                     EGVLGEKSCTLRLRGQGSYDERYMLPHQP"
     exon            430..580
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            581..691
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            692..891
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1112
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1191
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1279
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1453
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1590
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1683
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1774
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1937
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2130
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2189
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2358
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2452
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2583
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2584..2742
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2882
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..3036
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3147
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3148..3278
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..3461
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3462..3532
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..3684
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3685..3803
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3874
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..4080
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4081..4197
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4198..4323
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4324..4418
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4419..4503
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4504..4722
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4723..4884
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4885..5038
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            5039..5162
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            5163..6899
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5587..5592
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="hexamer: GATAAA"
     polyA_site      5606
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="major polyA site"
     regulatory      6402..6407
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="hexamer: AATATA"
     polyA_site      6423
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
ORIGIN      
        1 agcggcgtct cggttgccat ggagaccagg agctccaaaa cgcggaggtc tttagcgtcc
       61 cggaccaacg agtgccaggg gacaatgtgg gcgccaactt cgccaccagc cgggtccagc
      121 agccccagcc agcccacctg gaagtcctcc ttgtattcct ccctcgccta ctctgaggcc
      181 ttccactaca gcttcgcagc ccggccccgc cgcctcacgc agcttgcgct ggcgcagcgt
      241 cccgagcctc agctgcttcg tctgcgcccc tcctcgctgc gcacccaaga tatctcgcac
      301 ttgctcaccg gcgtcttccg caacttgtac tcagccgagg tcatcggcga cgaagtgagc
      361 gcaagcttga tcaaggcccg cggcagcgag aatgagcgcc acgaggagtt cgtggaccag
      421 ctgcagcaga ttcgggagct ctataagcag cggctggatg agtttgaaat gttggagaga
      481 catatcactc aggcccaagc acgggctatt gcggaaaatg agcgggtcat gagccaggct
      541 ggagtacagg acctcgagag ccttgtcagg ttgcctccag tgaagagtgt ctccagatgg
      601 tgtatagaca gcgagttgct acggaaacat catttgatct ccccagaaga ttactacacc
      661 gatacagtgc cgtttcactc tgcacctaaa ggcatctccc tacctggatg ttcaaaactg
      721 acatttagct gtgagaagcg ttccgtccag aagaaagagc tgaacaagaa gcttgaagat
      781 tcatgcagga agaagcttgc tgagttcgaa gatgagttag accacactgt ggacagcctg
      841 acatggaatt taactcctaa ggccaaagaa aggaccagag aacctctcaa gaaagcaagt
      901 caaccaagga ataaaaactg gatgaaccac ttacgtgtgc cacagagaga gctagacaga
      961 cttctgcttg ccagaatgga gagtcggaac cacttcctaa aaaatccccg tttttttcct
     1021 cctaacactc gatatggagg caagtctctt gtttttcctc caaagaagcc agcaccgata
     1081 ggagaattcc agagtacaga gccagaacag agttgtgctg atactccagt gtttctagct
     1141 aagccaccaa ttgggttttt cacagattat gaaattggtc cagtttatga gatggtaatt
     1201 gcgctgcaga acaccaccac gaccagccgc tacctgcgag tcctcccgcc ttccacgcca
     1261 tacttcgctc tgggactggg gatgttccca ggaaaaggtg gaatggtggc tcctggaatg
     1321 acctgccagt acattgtcca gttttttccc gactgccttg gggattttga tgattttatt
     1381 ttagtggaga cccagtcagc ccacacactt ctgatccccc tgcaggcccg gaggccgccc
     1441 cccgtgctga cattgtcacc ggtgttggac tgtggttact gcctcattgg gggagtcaag
     1501 atgaccagat tcatctgcaa aaatgtgggt ttcagtgttg gcaggttctg cattatgccc
     1561 aaaacaagct ggccaccact aagtttcaag gccattgcaa ccgtcggctt tgttgaacaa
     1621 cctccttttg gaatcctgcc ttcggtgttt gagctggccc cgggacatgc tatattagtg
     1681 gaggtcttgt tttccccaaa gagcctagga aaggcagagc agaccttcat catcatgtgc
     1741 gacaactgcc agataaagga gctggtgacc ataggaattg ggcagctgat tgctttggat
     1801 ctgatctata tttctggtga aaaaagccag ccagaccctg gagagctcac agacttaaca
     1861 gcccagcact tcatacgatt tgagcctgaa aaccttcggt ccacggctag gaagcagctg
     1921 attattagaa atgctacgca cgtggagctg gccttctact ggcagatcat gaagcccaac
     1981 ctgcagcccc tcatgcctgg agaaaccttc agcatggaca gcatcaagtg ctaccccgac
     2041 aaggagactg ccttctccat catgcccaga aagggggttc taagccccca cacagaccac
     2101 gagttcatcc tgagcttttc tcctcatgag ctgagggatt ttcacagtgt gctccagatg
     2161 gtgctagagg aagtcccaga gcctgtaagt tcagaagcgg agagcctggg gcactcctcc
     2221 tactctgtgg atgatgtgat tgtcctggaa atcgaggtga aaggctcagt agaacctttc
     2281 caggttctct tagagccata tgccctcatc atcccagggg agaactacat tgggataaat
     2341 gtgaagaagg cttttaagat gtggaacaac agcaagtcac ccatcagata cctgtggggg
     2401 aagatcagcg actgccacat cattgaagtg gagcccggca caggggtcat agagcccagt
     2461 gaggtcgggg attttgagtt gaactttact gggggtgtcc ctggccccac aagccaggac
     2521 ctgctgtgtg aaatcgaaga ctcgccctcg ccagtggtgt tacacattga ggctgtcttt
     2581 aaggggcctg ccctcatcat caacgtctca gcccttcagt ttggtctgct ccgcctgggg
     2641 cagaaagcca caaactccat ccagatccgg aacgtcagcc agctcccagc cacatggcgc
     2701 atgaaggaga gcccagtctc cctccaggaa aggcctgagg atgtgtctcc cttcgacatt
     2761 gagccttcga gtggccagct tcactctctg ggggagtgca gggtggacat caccttggag
     2821 gccctgcact gccagcatct ggagaccgtc ctggagctgg aggtggaaaa tggtgcctgg
     2881 agctaccttc ctgtgtatgc tgaggtacag aagccccatg tgtacctaca gagcagccag
     2941 gtggaggtta gaaatctcta cctgggtgtg cccacgaaga caaccatcac acttatcaat
     3001 ggcacgctcc tgcctaccca gttccactgg ggcaagctcc tcggacacca agcagaattc
     3061 tgcatggtga cagtctcccc caaacatggc ctgctgggcc caagtgagga gtgccagctc
     3121 aagttggagt tgactgctca tacccaggaa gagctgaccc atctggccct cccttgtcac
     3181 gtgtcaggca tgaagaagcc actggttcta ggcatttctg ggaagcccca gggactgcaa
     3241 gtggccatta ccatctctaa ggagagctct gattgcagca cagagcagtg gccaggccac
     3301 ccaaaggagc tccgcctgga ctttggctca gcggtgccac tgaggacccg tgtgactcgc
     3361 cagctcattc tcaccaatcg ctccccaata cggacccgtt tctccctcaa gtttgagtat
     3421 ttcgggagcc cccaaaacag cctgagcaaa aagaccagcc ttcccaacat gcctcctgcc
     3481 ctgctaaaga cagtgcggat gcaagagcac ctggccaagc gagagcagct ggattttatg
     3541 gagagcatgc tatcccacgg gaaaggagct gctttcttcc ctcacttttc ccagggcatg
     3601 ctggggccct accagcagct gtgcattgac atcacaggct gtgccaacat gtggggcgag
     3661 tactgggaca acctcatctg cacggtggga gacctgctgc cggaagtcat cccagtgcac
     3721 atggcagcgg tgggctgccc catcagctcc ctgaggacca cctcctacac tattgaccag
     3781 gcccagaagg aaccagccat gaggttcggc acccaggtct ccggaggaga cacagttacc
     3841 cgaacccttc gcctgaataa ctccagcccc tgtgacatcc gcctggattg ggagacctat
     3901 gttccagaag acaaggaaga ccggctggtg gagctgctgg tgttttatgg gccacctttc
     3961 ccgctgcggg accaagccgg gaatgagctt gtgtgccctg atacccctga gggtggctgc
     4021 ctcctctggt ccccaggccc ctccagttca tcggaattca gccatgaaac tgactcatca
     4081 gttgagggca gctccagtgc cagcaatagg gtggcacaga agctcatctc agtcatcctg
     4141 caggcacatg agggggtgcc ctccggccac ctgtactgta tcagccccaa gcaggtggtg
     4201 gtccctgctg ggggcagcag taccatctac atctccttca cccctatggt gctcagccct
     4261 gagatcctgc acaaggtgga gtgtactggc tacgccctgg gtttcatgag cttggacagc
     4321 aaggtggaaa gggagattcc agggaagagg catcgcctgc aggactttgc ggtgggaccc
     4381 ctgaaactgg acctgcatag ctacgtgagg cctgcacagc taagtgtgga gctggactac
     4441 ggcggcagta tggaattcca gtgccaggcc agtgacctca ttcccgagca gccctgctct
     4501 ggggtgctga gtgagctggt gaccacccac cacctgaagc tgaccaacac tacagagatc
     4561 ccacactact tccggcttat ggtctccagg cccttctccg tttctcaaga tggggcgagc
     4621 caggaccaca gagctcctgg ccctggccag aagcaggagt gtgaggagga gacagcctca
     4681 gcggacaagc agctggtgct ccaagcacag gagaacatgc tggtgaacgt gtccttctca
     4741 ctctccctgg agctgctctc ctatcagaag ctcccagctg accagacact gcctggggtg
     4801 gacattcagc agagtgcgag tggagagaga gagatggtgt ttactcagaa cctgctcctg
     4861 gagtacacca accagaccac tcaggtggtg cccctgcggg ctgtggtggc cgtgcctgag
     4921 ctgcagctct ccaccagctg ggtggacttt gggacctgct ttgtgagcca gcagcgagtc
     4981 cgggaggtct acctgatgaa cctgagcggg tgccgaagct actggactat gctgatgggc
     5041 cagcaggagc cagccaaggc cgctgtggcc ttcagggtct ccccaaacag tgggctgcta
     5101 gaagcacgat ccgccaatgc acccccaacc tccatcgcct tgcaggtttt cttcactgcc
     5161 aggagtagtg agctgtacga gtccacgatg gtggtggaag gtgtgctcgg tgagaagtcc
     5221 tgcaccctgc ggctccgggg ccaaggctcc tatgatgaga gatacatgtt gcctcaccag
     5281 ccctgaggct ccgccccagc cctcagcccc aggccccagc tggagaaaaa acattgccca
     5341 gggattagga gcagctcttc agcacaaaga cacagacttg gggacctggg gacctctggg
     5401 cagctcctgg aatggaagaa cccccttcca caatggtctc agcctaggcc ctcatgatat
     5461 gtcctcagag ctaacataaa ggacaggcca caccacagca gagaccacca cattgagatc
     5521 actactcagt gcatagcgaa gaccagtatg gcaaaattag tcttggaaaa aaaccacagc
     5581 cactaagata aattcatgca cttttactat gcccattgca cttctcatcc atggatttgc
     5641 cttgccttaa gaattaacca tggccttgtg gcctgggtga cccaggctgc ttttatcttg
     5701 cacagctaaa gagggtctga tgggtggctc aacaccccac ccactcctat accatgtcat
     5761 cagtgttcac attttccaaa tgagttgaag tgccttgatc tgtccactgc caccaccacc
     5821 agtgctgagt tttcccatgt ggttttgctt ttgtggtgtt actgccttgc tgctagagca
     5881 gcaggactgt ctgcgtagcg cctccagcct gggacctcac tcagttccca gggtattcaa
     5941 agacggcagc ggctcccatt ccagtcccga tgcacatgga caccactccg tatgccctgt
     6001 gaaaacaaaa cttaattggc tgggcaaagg cacccaccaa attacctcca gaccaggctg
     6061 acccagaagg acgtcatgga caagtaggat gcaaaaccat cagaccagat ctgtgggcaa
     6121 gcggtaccct ggcctcccac ttgggcacac acacggtgac agatgcccac tgcaggctag
     6181 tatccctttc aaggctggcc cttaaggaaa acagggatca tgcccctaca tcctaagttc
     6241 agggtgtttc tgtgtgcatg ttcccctccc cagggatcga tcataatccc aaaagacaca
     6301 atcccaaaca caatcatccc aaatgttgaa atcctggaag aacaaaatcc ctaaagtcta
     6361 aaatcctgaa aatcacaatc ctaaaagatc aaaatcctga aaatataatt ctggaaaaaa
     6421 taatttggaa gcctggcctg gtggcatcta cttgtagtcc cagctattca gcaggctgag
     6481 gcaagaggat cacttgtgcc taggagttca agaccagcct aggcaatata gcaagacccc
     6541 atcttagaaa aaaacttttt taaaagattg atttaaaaga tatttacatt tttaggccag
     6601 gtgtggtggc tcaaacctgt aatcccaaca ctttgggagg ccgaggcggg aggatcacga
     6661 ggtcaggagt tcaagaccag cctcgccaac atggtgaaac cctgtctcca ccaaaaatac
     6721 aaaaattagc tgggtgtggt ggcagacacc tataatccca gatactcggg aggctgaggc
     6781 tgaggcagga aaattgcttg aacccaaaag gcagaggttg cagtgagccg agatcacacc
     6841 actgcactcc agccagcctg gacaacggag tgagattcca tgtcaaaaaa aaaaaaaaa
//
