LOCUS       NM_001321153            6908 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DLEC1 cilia and flagella associated protein (DLEC1),
            transcript variant 3, mRNA.
ACCESSION   NM_001321153 XM_005265630
VERSION     NM_001321153.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Chou,Y.H., Tantoh,D.M., Wu,M.C., Tyan,Y.S., Chen,P.H., Nfor,O.N.,
            Hsu,S.Y., Shen,C.Y., Huang,C.N. and Liaw,Y.P.
  TITLE     PM2.5 exposure and DLEC1 promoter methylation in Taiwan Biobank
            participants
  JOURNAL   Environ Health Prev Med 25 (1), 68 (2020)
   PUBMED   33153431
  REMARK    GeneRIF: PM2.5 exposure and DLEC1 promoter methylation in Taiwan
            Biobank participants.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Okitsu,Y., Nagano,M., Yamagata,T., Ito,C., Toshimori,K., Dohra,H.,
            Fujii,W. and Yogo,K.
  TITLE     Dlec1 is required for spermatogenesis and male fertility in mice
  JOURNAL   Sci Rep 10 (1), 18883 (2020)
   PUBMED   33144677
  REMARK    GeneRIF: Dlec1 is required for spermatogenesis and male fertility
            in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6908)
  AUTHORS   Xie,W., Zhou,H., Han,Q., Sun,T., Nie,C., Hong,J., Wei,R.,
            Leonteva,A., Han,X., Wang,J., Du,X., Zhu,L., Zhao,Y., Tian,W. and
            Xue,Y.
  TITLE     Relationship between DLEC1 and PBX3 promoter methylation and the
            risk and prognosis of gastric cancer in peripheral blood leukocytes
  JOURNAL   J Cancer Res Clin Oncol 146 (5), 1115-1124 (2020)
   PUBMED   32144534
  REMARK    GeneRIF: Compared with negative methylation (Nm), DLEC1-positive
            methylation (Pm) was associated with increased GC risk in PS (OR
            2.083, 95% CI 1.220-3.558, P = 0.007), but PBX3 Pm was not
            associated with GC risk.
REFERENCE   4  (bases 1 to 6908)
  AUTHORS   Brany,D., Dvorska,D., Grendar,M., Nachajova,M., Szepe,P.,
            Lasabova,Z., Zubor,P., Visnovsky,J. and Halasova,E.
  TITLE     Different methylation levels in the KLF4, ATF3 and DLEC1 genes in
            the myometrium and in corpus uteri mesenchymal tumours as assessed
            by MS-HRM
  JOURNAL   Pathol Res Pract 215 (8), 152465 (2019)
   PUBMED   31176573
  REMARK    GeneRIF: that the KLF4 and DLEC1 genes can be considered potential
            methylation biomarkers for uterine leiomyomas
REFERENCE   5  (bases 1 to 6908)
  AUTHORS   Kim,Y., Lee,K., Jeong,S., Wen,X., Cho,N.Y. and Kang,G.H.
  TITLE     DLEC1 methylation is associated with a better clinical outcome in
            patients with intrahepatic cholangiocarcinoma of the small duct
            subtype
  JOURNAL   Virchows Arch 475 (1), 49-58 (2019)
   PUBMED   30610381
  REMARK    GeneRIF: DLEC1 methylation can be utilized to identify a subset
            with a better prognosis in intrahepatic cholangiocarcinomas of the
            small duct type.
REFERENCE   6  (bases 1 to 6908)
  AUTHORS   Seng,T.J., Currey,N., Cooper,W.A., Lee,C.S., Chan,C., Horvath,L.,
            Sutherland,R.L., Kennedy,C., McCaughan,B. and Kohonen-Corish,M.R.
  TITLE     DLEC1 and MLH1 promoter methylation are associated with poor
            prognosis in non-small cell lung carcinoma
  JOURNAL   Br J Cancer 99 (2), 375-382 (2008)
   PUBMED   18594535
  REMARK    GeneRIF: DLEC1 methylation was an independent marker of poor
            survival in patients with non-small cell lung carcinoma.
REFERENCE   7  (bases 1 to 6908)
  AUTHORS   Qiu,G.H., Salto-Tellez,M., Ross,J.A., Yeo,W., Cui,Y.,
            Wheelhouse,N., Chen,G.G., Harrison,D., Lai,P., Tao,Q. and Hooi,S.C.
  TITLE     The tumor suppressor gene DLEC1 is frequently silenced by DNA
            methylation in hepatocellular carcinoma and induces G1 arrest in
            cell cycle
  JOURNAL   J Hepatol 48 (3), 433-441 (2008)
   PUBMED   18191269
  REMARK    GeneRIF: DLEC1 is a candidate tumor suppressor gene that plays an
            important role in the development and progression of hepatocellular
            carcinoma.
REFERENCE   8  (bases 1 to 6908)
  AUTHORS   Kwong,J., Chow,L.S., Wong,A.Y., Hung,W.K., Chung,G.T., To,K.F.,
            Chan,F.L., Daigo,Y., Nakamura,Y., Huang,D.P. and Lo,K.W.
  TITLE     Epigenetic inactivation of the deleted in lung and esophageal
            cancer 1 gene in nasopharyngeal carcinoma
  JOURNAL   Genes Chromosomes Cancer 46 (2), 171-180 (2007)
   PUBMED   17099870
  REMARK    GeneRIF: Silencing of DLEC1 expression by promoter hypermethylation
            and histone deacetylation may be important in nasopharyngeal
            carcinoma tumorigenesis.
REFERENCE   9  (bases 1 to 6908)
  AUTHORS   Kwong,J., Lee,J.Y., Wong,K.K., Zhou,X., Wong,D.T., Lo,K.W.,
            Welch,W.R., Berkowitz,R.S. and Mok,S.C.
  TITLE     Candidate tumor-suppressor gene DLEC1 is frequently downregulated
            by promoter hypermethylation and histone hypoacetylation in human
            epithelial ovarian cancer
  JOURNAL   Neoplasia 8 (4), 268-278 (2006)
   PUBMED   16756719
  REMARK    GeneRIF: DLEC1 suppresses the growth of ovarian cancer cells and
            its downregulation is closely associated with promoter
            hypermethylation and histone hypoacetylation
REFERENCE   10 (bases 1 to 6908)
  AUTHORS   Daigo,Y., Nishiwaki,T., Kawasoe,T., Tamari,M., Tsuchiya,E. and
            Nakamura,Y.
  TITLE     Molecular cloning of a candidate tumor suppressor gene, DLC1, from
            chromosome 3p21.3
  JOURNAL   Cancer Res 59 (8), 1966-1972 (1999)
   PUBMED   10213508
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB020522.1, AB425198.1,
            CB243860.1 and AP006309.1.
            
            On May 31, 2019 this sequence version replaced NM_001321153.1.
            
            Summary: The cytogenetic location of this gene is 3p21.3, and it is
            located in a region that is commonly deleted in a variety of
            malignancies. Down-regulation of this gene has been observed in
            several human cancers including lung, esophageal, renal tumors, and
            head and neck squamous cell carcinoma. In some cases, reduced
            expression of this gene in tumor cells is a result of aberrant
            promoter methylation. Several alternatively spliced transcripts
            have been observed that contain disrupted coding regions and likely
            encode nonfunctional proteins.[provided by RefSeq, Mar 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB425198.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AB020522.1         4-28
            26-5617             AB425198.1         1-5592
            5618-5624           CB243860.1         18-24               c
            5625-6908           AP006309.1         103222-104505
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..6908
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="DLEC1 cilia and flagella associated protein"
                     /db_xref="GeneID:9940"
                     /db_xref="HGNC:HGNC:2899"
                     /db_xref="MIM:604050"
     exon            1..429
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..5295
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="isoform 3 is encoded by transcript variant 3; cilia
                     and flagella associated protein 81; DLEC, cilia and
                     flagella associated protein; deleted in lung and
                     esophageal cancer 1"
                     /codon_start=1
                     /product="deleted in lung and esophageal cancer protein 1
                     isoform 3"
                     /protein_id="NP_001308082.1"
                     /db_xref="GeneID:9940"
                     /db_xref="HGNC:HGNC:2899"
                     /db_xref="MIM:604050"
                     /translation="METRSSKTRRSLASRTNECQGTMWAPTSPPAGSSSPSQPTWKSS
                     LYSSLAYSEAFHYSFAARPRRLTQLALAQRPEPQLLRLRPSSLRTQDISHLLTGVFRN
                     LYSAEVIGDEVSASLIKARGSENERHEEFVDQLQQIRELYKQRLDEFEMLERHITQAQ
                     ARAIAENERVMSQAGVQDLESLVRLPPVKSVSRWCIDSELLRKHHLISPEDYYTDTVP
                     FHSAPKGISLPGCSKLTFSCEKRSVQKKELNKKLEDSCRKKLAEFEDELDHTVDSLTW
                     NLTPKAKERTREPLKKASQPRNKNWMNHLRVPQRELDRLLLARMESRNHFLKNPRFFP
                     PNTRYGGKSLVFPPKKPAPIGEFQSTEPEQSCADTPVFLAKPPIGFFTDYEIGPVYEM
                     VIALQNTTTTSRYLRVLPPSTPYFALGLGMFPGKGGMVAPGMTCQYIVQFFPDCLGDF
                     DDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGVKMTRFICKNVGFSVG
                     RFCIMPKTSWPPLSFKAIATVGFVEQPPFGILPSVFELAPGHAILVEVLFSPKSLGKA
                     EQTFIIMCDNCQIKELVTIGIGQLIALDLIYISGEKSQPDPGELTDLTAQHFIRFEPE
                     NLRSTARKQLIIRNATHVELAFYWQIMKPNLQPLMPGETFSMDSIKCYPDKETAFSIM
                     PRKGVLSPHTDHEFILSFSPHELRDFHSVLQMVLEEVPEPVSSEAESLGHSSYSVDDV
                     IVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKAFKMWNNSKSPIRYLWGKISD
                     CHIIEVEPGTGVIEPSEVGDFELNFTGGVPGPTSQDLLCEIEDSPSPVVLHIEAVFKG
                     PALIINVSALQFGLLRLGQKATNSIQIRNVSQLPATWRMKESPVSLQERPEDVSPFDI
                     EPSSGQLHSLGECRVDITLEALHCQHLETVLELEVENGAWSYLPVYAEVQKPHVYLQS
                     SQVEVRNLYLGVPTKTTITLINGTLLPTQFHWGKLLGHQAEFCMVTVSPKHGLLGPSE
                     ECQLKLELTAHTQEELTHLALPCHVSGMKKPLVLGISGKPQGLQVAITISKESSDCSV
                     FSTEQWPGHPKELRLDFGSAVPLRTRVTRQLILTNRSPIRTRFSLKFEYFGSPQNSLS
                     KKTSLPNMPPALLKTVRMQEHLAKREQLDFMESMLSHGKGAAFFPHFSQGMLGPYQQL
                     CIDITGCANMWGEYWDNLICTVGDLLPEVIPVHMAAVGCPISSLRTTSYTIDQAQKEP
                     AMRFGTQVSGGDTVTRTLRLNNSSPCDIRLDWETYVPEDKEDRLVELLVFYGPPFPLR
                     DQAGNELVCPDTPEGGCLLWSPGPSSSSEFSHETDSSVEGSSSASNRVAQKLISVILQ
                     AHEGVPSGHLYCISPKQVVVPAGGSSTIYISFTPMVLSPEILHKVECTGYALGFMSLD
                     SKVEREIPGKRHRLQDFAVGPLKLDLHSYVRPAQLSVELDYGGSMEFQCQASDLIPEQ
                     PCSGVLSELVTTHHLKLTNTTEIPHYFRLMVSRPFSVSQDGASQDHRAPGPGQKQECE
                     EETASADKQLVLQAQENMLVNVSFSLSLELLSYQKLPADQTLPGVDIQQSASGEREMV
                     FTQNLLLEYTNQTTQVVPLRAVVAVPELQLSTSWVDFGTCFVSQQRVREVYLMNLSGC
                     RSYWTMLMGQQEPAKAAVAFRVSPNSGLLEARSANAPPTSIALQVFFTARSSELYEST
                     MVVEGVLGEKSCTLRLRGQGSYDERYMLPHQP"
     exon            430..580
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            581..691
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            692..891
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1112
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1191
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1279
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1453
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1590
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1683
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1774
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1937
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2130
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2189
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2358
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2452
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2583
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2584..2742
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2882
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..3036
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3147
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3148..3278
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..3470
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3471..3541
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3693
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3812
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3813..3883
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..4089
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4090..4206
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4207..4332
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4333..4427
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4428..4512
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4513..4731
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4732..4893
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            4894..5047
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            5048..5171
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     exon            5172..6908
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5596..5601
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="hexamer: GATAAA"
     polyA_site      5615
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="major polyA site"
     regulatory      6411..6416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
                     /note="hexamer: AATATA"
     polyA_site      6432
                     /gene="DLEC1"
                     /gene_synonym="CFAP81; DLC-1; DLC1; F56; FAP81"
ORIGIN      
        1 agcggcgtct cggttgccat ggagaccagg agctccaaaa cgcggaggtc tttagcgtcc
       61 cggaccaacg agtgccaggg gacaatgtgg gcgccaactt cgccaccagc cgggtccagc
      121 agccccagcc agcccacctg gaagtcctcc ttgtattcct ccctcgccta ctctgaggcc
      181 ttccactaca gcttcgcagc ccggccccgc cgcctcacgc agcttgcgct ggcgcagcgt
      241 cccgagcctc agctgcttcg tctgcgcccc tcctcgctgc gcacccaaga tatctcgcac
      301 ttgctcaccg gcgtcttccg caacttgtac tcagccgagg tcatcggcga cgaagtgagc
      361 gcaagcttga tcaaggcccg cggcagcgag aatgagcgcc acgaggagtt cgtggaccag
      421 ctgcagcaga ttcgggagct ctataagcag cggctggatg agtttgaaat gttggagaga
      481 catatcactc aggcccaagc acgggctatt gcggaaaatg agcgggtcat gagccaggct
      541 ggagtacagg acctcgagag ccttgtcagg ttgcctccag tgaagagtgt ctccagatgg
      601 tgtatagaca gcgagttgct acggaaacat catttgatct ccccagaaga ttactacacc
      661 gatacagtgc cgtttcactc tgcacctaaa ggcatctccc tacctggatg ttcaaaactg
      721 acatttagct gtgagaagcg ttccgtccag aagaaagagc tgaacaagaa gcttgaagat
      781 tcatgcagga agaagcttgc tgagttcgaa gatgagttag accacactgt ggacagcctg
      841 acatggaatt taactcctaa ggccaaagaa aggaccagag aacctctcaa gaaagcaagt
      901 caaccaagga ataaaaactg gatgaaccac ttacgtgtgc cacagagaga gctagacaga
      961 cttctgcttg ccagaatgga gagtcggaac cacttcctaa aaaatccccg tttttttcct
     1021 cctaacactc gatatggagg caagtctctt gtttttcctc caaagaagcc agcaccgata
     1081 ggagaattcc agagtacaga gccagaacag agttgtgctg atactccagt gtttctagct
     1141 aagccaccaa ttgggttttt cacagattat gaaattggtc cagtttatga gatggtaatt
     1201 gcgctgcaga acaccaccac gaccagccgc tacctgcgag tcctcccgcc ttccacgcca
     1261 tacttcgctc tgggactggg gatgttccca ggaaaaggtg gaatggtggc tcctggaatg
     1321 acctgccagt acattgtcca gttttttccc gactgccttg gggattttga tgattttatt
     1381 ttagtggaga cccagtcagc ccacacactt ctgatccccc tgcaggcccg gaggccgccc
     1441 cccgtgctga cattgtcacc ggtgttggac tgtggttact gcctcattgg gggagtcaag
     1501 atgaccagat tcatctgcaa aaatgtgggt ttcagtgttg gcaggttctg cattatgccc
     1561 aaaacaagct ggccaccact aagtttcaag gccattgcaa ccgtcggctt tgttgaacaa
     1621 cctccttttg gaatcctgcc ttcggtgttt gagctggccc cgggacatgc tatattagtg
     1681 gaggtcttgt tttccccaaa gagcctagga aaggcagagc agaccttcat catcatgtgc
     1741 gacaactgcc agataaagga gctggtgacc ataggaattg ggcagctgat tgctttggat
     1801 ctgatctata tttctggtga aaaaagccag ccagaccctg gagagctcac agacttaaca
     1861 gcccagcact tcatacgatt tgagcctgaa aaccttcggt ccacggctag gaagcagctg
     1921 attattagaa atgctacgca cgtggagctg gccttctact ggcagatcat gaagcccaac
     1981 ctgcagcccc tcatgcctgg agaaaccttc agcatggaca gcatcaagtg ctaccccgac
     2041 aaggagactg ccttctccat catgcccaga aagggggttc taagccccca cacagaccac
     2101 gagttcatcc tgagcttttc tcctcatgag ctgagggatt ttcacagtgt gctccagatg
     2161 gtgctagagg aagtcccaga gcctgtaagt tcagaagcgg agagcctggg gcactcctcc
     2221 tactctgtgg atgatgtgat tgtcctggaa atcgaggtga aaggctcagt agaacctttc
     2281 caggttctct tagagccata tgccctcatc atcccagggg agaactacat tgggataaat
     2341 gtgaagaagg cttttaagat gtggaacaac agcaagtcac ccatcagata cctgtggggg
     2401 aagatcagcg actgccacat cattgaagtg gagcccggca caggggtcat agagcccagt
     2461 gaggtcgggg attttgagtt gaactttact gggggtgtcc ctggccccac aagccaggac
     2521 ctgctgtgtg aaatcgaaga ctcgccctcg ccagtggtgt tacacattga ggctgtcttt
     2581 aaggggcctg ccctcatcat caacgtctca gcccttcagt ttggtctgct ccgcctgggg
     2641 cagaaagcca caaactccat ccagatccgg aacgtcagcc agctcccagc cacatggcgc
     2701 atgaaggaga gcccagtctc cctccaggaa aggcctgagg atgtgtctcc cttcgacatt
     2761 gagccttcga gtggccagct tcactctctg ggggagtgca gggtggacat caccttggag
     2821 gccctgcact gccagcatct ggagaccgtc ctggagctgg aggtggaaaa tggtgcctgg
     2881 agctaccttc ctgtgtatgc tgaggtacag aagccccatg tgtacctaca gagcagccag
     2941 gtggaggtta gaaatctcta cctgggtgtg cccacgaaga caaccatcac acttatcaat
     3001 ggcacgctcc tgcctaccca gttccactgg ggcaagctcc tcggacacca agcagaattc
     3061 tgcatggtga cagtctcccc caaacatggc ctgctgggcc caagtgagga gtgccagctc
     3121 aagttggagt tgactgctca tacccaggaa gagctgaccc atctggccct cccttgtcac
     3181 gtgtcaggca tgaagaagcc actggttcta ggcatttctg ggaagcccca gggactgcaa
     3241 gtggccatta ccatctctaa ggagagctct gattgcagtg ttttcagcac agagcagtgg
     3301 ccaggccacc caaaggagct ccgcctggac tttggctcag cggtgccact gaggacccgt
     3361 gtgactcgcc agctcattct caccaatcgc tccccaatac ggacccgttt ctccctcaag
     3421 tttgagtatt tcgggagccc ccaaaacagc ctgagcaaaa agaccagcct tcccaacatg
     3481 cctcctgccc tgctaaagac agtgcggatg caagagcacc tggccaagcg agagcagctg
     3541 gattttatgg agagcatgct atcccacggg aaaggagctg ctttcttccc tcacttttcc
     3601 cagggcatgc tggggcccta ccagcagctg tgcattgaca tcacaggctg tgccaacatg
     3661 tggggcgagt actgggacaa cctcatctgc acggtgggag acctgctgcc ggaagtcatc
     3721 ccagtgcaca tggcagcggt gggctgcccc atcagctccc tgaggaccac ctcctacact
     3781 attgaccagg cccagaagga accagccatg aggttcggca cccaggtctc cggaggagac
     3841 acagttaccc gaacccttcg cctgaataac tccagcccct gtgacatccg cctggattgg
     3901 gagacctatg ttccagaaga caaggaagac cggctggtgg agctgctggt gttttatggg
     3961 ccacctttcc cgctgcggga ccaagccggg aatgagcttg tgtgccctga tacccctgag
     4021 ggtggctgcc tcctctggtc cccaggcccc tccagttcat cggaattcag ccatgaaact
     4081 gactcatcag ttgagggcag ctccagtgcc agcaataggg tggcacagaa gctcatctca
     4141 gtcatcctgc aggcacatga gggggtgccc tccggccacc tgtactgtat cagccccaag
     4201 caggtggtgg tccctgctgg gggcagcagt accatctaca tctccttcac ccctatggtg
     4261 ctcagccctg agatcctgca caaggtggag tgtactggct acgccctggg tttcatgagc
     4321 ttggacagca aggtggaaag ggagattcca gggaagaggc atcgcctgca ggactttgcg
     4381 gtgggacccc tgaaactgga cctgcatagc tacgtgaggc ctgcacagct aagtgtggag
     4441 ctggactacg gcggcagtat ggaattccag tgccaggcca gtgacctcat tcccgagcag
     4501 ccctgctctg gggtgctgag tgagctggtg accacccacc acctgaagct gaccaacact
     4561 acagagatcc cacactactt ccggcttatg gtctccaggc ccttctccgt ttctcaagat
     4621 ggggcgagcc aggaccacag agctcctggc cctggccaga agcaggagtg tgaggaggag
     4681 acagcctcag cggacaagca gctggtgctc caagcacagg agaacatgct ggtgaacgtg
     4741 tccttctcac tctccctgga gctgctctcc tatcagaagc tcccagctga ccagacactg
     4801 cctggggtgg acattcagca gagtgcgagt ggagagagag agatggtgtt tactcagaac
     4861 ctgctcctgg agtacaccaa ccagaccact caggtggtgc ccctgcgggc tgtggtggcc
     4921 gtgcctgagc tgcagctctc caccagctgg gtggactttg ggacctgctt tgtgagccag
     4981 cagcgagtcc gggaggtcta cctgatgaac ctgagcgggt gccgaagcta ctggactatg
     5041 ctgatgggcc agcaggagcc agccaaggcc gctgtggcct tcagggtctc cccaaacagt
     5101 gggctgctag aagcacgatc cgccaatgca cccccaacct ccatcgcctt gcaggttttc
     5161 ttcactgcca ggagtagtga gctgtacgag tccacgatgg tggtggaagg tgtgctcggt
     5221 gagaagtcct gcaccctgcg gctccggggc caaggctcct atgatgagag atacatgttg
     5281 cctcaccagc cctgaggctc cgccccagcc ctcagcccca ggccccagct ggagaaaaaa
     5341 cattgcccag ggattaggag cagctcttca gcacaaagac acagacttgg ggacctgggg
     5401 acctctgggc agctcctgga atggaagaac ccccttccac aatggtctca gcctaggccc
     5461 tcatgatatg tcctcagagc taacataaag gacaggccac accacagcag agaccaccac
     5521 attgagatca ctactcagtg catagcgaag accagtatgg caaaattagt cttggaaaaa
     5581 aaccacagcc actaagataa attcatgcac ttttactatg cccattgcac ttctcatcca
     5641 tggatttgcc ttgccttaag aattaaccat ggccttgtgg cctgggtgac ccaggctgct
     5701 tttatcttgc acagctaaag agggtctgat gggtggctca acaccccacc cactcctata
     5761 ccatgtcatc agtgttcaca ttttccaaat gagttgaagt gccttgatct gtccactgcc
     5821 accaccacca gtgctgagtt ttcccatgtg gttttgcttt tgtggtgtta ctgccttgct
     5881 gctagagcag caggactgtc tgcgtagcgc ctccagcctg ggacctcact cagttcccag
     5941 ggtattcaaa gacggcagcg gctcccattc cagtcccgat gcacatggac accactccgt
     6001 atgccctgtg aaaacaaaac ttaattggct gggcaaaggc acccaccaaa ttacctccag
     6061 accaggctga cccagaagga cgtcatggac aagtaggatg caaaaccatc agaccagatc
     6121 tgtgggcaag cggtaccctg gcctcccact tgggcacaca cacggtgaca gatgcccact
     6181 gcaggctagt atccctttca aggctggccc ttaaggaaaa cagggatcat gcccctacat
     6241 cctaagttca gggtgtttct gtgtgcatgt tcccctcccc agggatcgat cataatccca
     6301 aaagacacaa tcccaaacac aatcatccca aatgttgaaa tcctggaaga acaaaatccc
     6361 taaagtctaa aatcctgaaa atcacaatcc taaaagatca aaatcctgaa aatataattc
     6421 tggaaaaaat aatttggaag cctggcctgg tggcatctac ttgtagtccc agctattcag
     6481 caggctgagg caagaggatc acttgtgcct aggagttcaa gaccagccta ggcaatatag
     6541 caagacccca tcttagaaaa aaactttttt aaaagattga tttaaaagat atttacattt
     6601 ttaggccagg tgtggtggct caaacctgta atcccaacac tttgggaggc cgaggcggga
     6661 ggatcacgag gtcaggagtt caagaccagc ctcgccaaca tggtgaaacc ctgtctccac
     6721 caaaaataca aaaattagct gggtgtggtg gcagacacct ataatcccag atactcggga
     6781 ggctgaggct gaggcaggaa aattgcttga acccaaaagg cagaggttgc agtgagccga
     6841 gatcacacca ctgcactcca gccagcctgg acaacggagt gagattccat gtcaaaaaaa
     6901 aaaaaaaa
//
