LOCUS       NM_182643               7412 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens DLC1 Rho GTPase activating protein (DLC1), transcript
            variant 1, mRNA.
ACCESSION   NM_182643
VERSION     NM_182643.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Hooglugt,A., van der Stoel,M.M., Shapeti,A., Neep,B.F., de Haan,A.,
            van Oosterwyck,H., Boon,R.A. and Huveneers,S.
  TITLE     DLC1 promotes mechanotransductive feedback for YAP via
            RhoGAP-mediated focal adhesion turnover
  JOURNAL   J Cell Sci 137 (8) (2024)
   PUBMED   38563084
  REMARK    GeneRIF: DLC1 promotes mechanotransductive feedback for YAP via
            RhoGAP-mediated focal adhesion turnover.
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Pan,J., Li,D., Fan,X., Cheng,J., Jin,S., Chen,P., Lin,H. and Li,Y.
  TITLE     Aberrant DNA Methylation Patterns of Deleted in Liver Cancer 1
            Isoforms in Hepatocellular Carcinoma
  JOURNAL   DNA Cell Biol 42 (3), 140-150 (2023)
   PUBMED   36917700
  REMARK    GeneRIF: Aberrant DNA Methylation Patterns of Deleted in Liver
            Cancer 1 Isoforms in Hepatocellular Carcinoma.
REFERENCE   3  (bases 1 to 7412)
  AUTHORS   Wang,C.S., Zhang,X.B., Zhu,X.T. and Chen,R.S.
  TITLE     NBR2/miR-561-5p/DLC1 axis inhibited the development of multiple
            myeloma by activating the AMPK/mTOR pathway to repress glycolysis
  JOURNAL   Neoplasma 69 (5), 1165-1174 (2022)
   PUBMED   35951458
  REMARK    GeneRIF: NBR2/miR-561-5p/DLC1 axis inhibited the development of
            multiple myeloma by activating the AMPK/mTOR pathway to repress
            glycolysis.
REFERENCE   4  (bases 1 to 7412)
  AUTHORS   Hinsenkamp,I., Kohler,J.P., Flachsenhaar,C., Hitkova,I.,
            Meessen,S.E., Gaiser,T., Wieland,T., Weiss,C., Rocken,C., Mowat,M.,
            Quante,M., Taxauer,K., Mejias-Luque,R., Gerhard,M., Vogelmann,R.,
            Meindl-Beinker,N., Ebert,M. and Burgermeister,E.
  TITLE     Functional antagonism between CagA and DLC1 in gastric cancer
  JOURNAL   Cell Death Discov 8 (1), 358 (2022)
   PUBMED   35963849
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7412)
  AUTHORS   Li,Z., Wang,Y., Liu,S., Li,W., Wang,Z., Jia,Z., Zhu,Z. and Bao,Y.
  TITLE     MiR-200a-3p promotes gastric cancer progression by targeting DLC-1
  JOURNAL   J Mol Histol 53 (1), 39-49 (2022)
   PUBMED   34751841
  REMARK    GeneRIF: MiR-200a-3p promotes gastric cancer progression by
            targeting DLC-1.
REFERENCE   6  (bases 1 to 7412)
  AUTHORS   Yuan,B.Z., Zhou,X., Durkin,M.E., Zimonjic,D.B., Gumundsdottir,K.,
            Eyfjord,J.E., Thorgeirsson,S.S. and Popescu,N.C.
  TITLE     DLC-1 gene inhibits human breast cancer cell growth and in vivo
            tumorigenicity
  JOURNAL   Oncogene 22 (3), 445-450 (2003)
   PUBMED   12545165
  REMARK    GeneRIF: Genomic deletion of DLC-1 was observed in 40% of breast
            tumors, whereas reduced levels of DLC-1 mRNA were seen in 70% of
            breast, 70% of colon, 50% of prostate tumor cell lines.DLC-1 gene
            plays a role in breast cancer by acting as a tumor suppressor gene
REFERENCE   7  (bases 1 to 7412)
  AUTHORS   Yuan,B.Z., Durkin,M.E. and Popescu,N.C.
  TITLE     Promoter hypermethylation of DLC-1, a candidate tumor suppressor
            gene, in several common human cancers
  JOURNAL   Cancer Genet Cytogenet 140 (2), 113-117 (2003)
   PUBMED   12645648
  REMARK    GeneRIF: Differences in promoter hypermethylation were seen in 12
            hepatocellular carcinoma, breast, colon, and prostate tumor cell
            lines with aberrant DLC-1 expression. Hypermethylation abrogates
            the function of the DLC-1 gene in a subset of these cancers.
REFERENCE   8  (bases 1 to 7412)
  AUTHORS   Wilson,P.J., McGlinn,E., Marsh,A., Evans,T., Arnold,J., Wright,K.,
            Biden,K., Young,J., Wainwright,B., Wicking,C. and
            Chenevix-Trench,G.
  TITLE     Sequence variants of DLC1 in colorectal and ovarian tumours
  JOURNAL   Hum Mutat 15 (2), 156-165 (2000)
   PUBMED   10649492
REFERENCE   9  (bases 1 to 7412)
  AUTHORS   Yuan,B.Z., Yang,Y., Keck-Waggoner,C.L., Zimonjic,D.B.,
            Thorgeirsson,S.S. and Popescu,N.C.
  TITLE     Assignment and cloning of mouse Arhgap7 to chromosome 8A4-B2, a
            conserved syntenic region of human chromosome 8p22-->p21
  JOURNAL   Cytogenet Cell Genet 87 (3-4), 189-190 (1999)
   PUBMED   10702663
REFERENCE   10 (bases 1 to 7412)
  AUTHORS   Yuan,B.Z., Miller,M.J., Keck,C.L., Zimonjic,D.B., Thorgeirsson,S.S.
            and Popescu,N.C.
  TITLE     Cloning, characterization, and chromosomal localization of a gene
            frequently deleted in human liver cancer (DLC-1) homologous to rat
            RhoGAP
  JOURNAL   Cancer Res 58 (10), 2196-2199 (1998)
   PUBMED   9605766
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA549448.1, BC054511.1,
            AC022844.8, AC015641.9 and AB051510.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_182643.2.
            
            Summary: This gene encodes a GTPase-activating protein (GAP) that
            is a member of the rhoGAP family of proteins which play a role in
            the regulation of small GTP-binding proteins. GAP family proteins
            participate in signaling pathways that regulate cell processes
            involved in cytoskeletal changes. This gene functions as a tumor
            suppressor gene in a number of common cancers, including prostate,
            lung, colorectal, and breast cancers. Multiple transcript variants
            due to alternative promoters and alternative splicing have been
            found for this gene.[provided by RefSeq, Apr 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). Variant 1
            represents a transcript that is found in low abundance. Variants 1
            and 6 both encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB051510.1, BC054511.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000276297.9/ ENSP00000276297.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-368               DA549448.1         70-437
            369-1137            BC054511.1         193-961
            1138-1138           AC022844.8         49606-49606
            1139-2195           BC054511.1         963-2019
            2196-2196           AC015641.9         83191-83191         c
            2197-2780           BC054511.1         2021-2604
            2781-5216           AB051510.1         2736-5171
            5217-5217           AC015641.9         68231-68231         c
            5218-5771           AB051510.1         5173-5726
            5772-5772           AC015641.9         67676-67676         c
            5773-7410           AB051510.1         5728-7365
            7411-7412           AC015641.9         66036-66037         c
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..7412
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /note="DLC1 Rho GTPase activating protein"
                     /db_xref="GeneID:10395"
                     /db_xref="HGNC:HGNC:2897"
                     /db_xref="MIM:604258"
     exon            1..250
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            251..1398
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     CDS             376..4962
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     StAR-related lipid transfer (START) domain containing 12;
                     Rho-GTPase-activating protein 7; rho GTPase-activating
                     protein 7; deleted in liver cancer 1 protein; START
                     domain-containing protein 12; rho-type GTPase-activating
                     protein 7; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="rho GTPase-activating protein 7 isoform 1"
                     /protein_id="NP_872584.2"
                     /db_xref="CCDS:CCDS5989.1"
                     /db_xref="GeneID:10395"
                     /db_xref="HGNC:HGNC:2897"
                     /db_xref="MIM:604258"
                     /translation="MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASM
                     EKDATLNVDRKEKCVSLPDCCHGSELRDFPGRPMGHLSKDVDENDSHEGEDQFLSLEA
                     STETLVHVSDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVS
                     SNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKD
                     IAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQNEFLDTPCTNRGLPLLKTDFGS
                     CLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQ
                     VRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSTSSSPSGTPTNLRRHVPD
                     LESGSESGADTISVNQTRVNLSSDTESTDLPSSTPVANSGTKPKTTAIQGISEKEKAE
                     IEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDFLDRDAIEALCRRLNTLNK
                     CAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGS
                     PDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISV
                     CSSSNLAGNDDSFGSLPSPKELSSFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHS
                     KHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALNGNRINVPMVRKRSVSNSTQT
                     SSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTFNNVVEQNFKNR
                     ESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENS
                     SDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVK
                     GMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLN
                     EPEEPSEIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNL
                     LQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRTG
                     QPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDV
                     ADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTL
                     LYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKD
                     LNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSAD
                     YQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVP
                     EEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTN
                     LPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPE
                     WYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR"
     exon            1399..1548
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1689
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1723
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1795
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1877
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1941
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..3365
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3542
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3543..3702
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3703..3901
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..4115
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4116..4230
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4231..4449
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4667
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4668..4841
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4842..7412
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7386..7391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /note="hexamer: AATAAA"
     polyA_site      7412
                     /gene="DLC1"
                     /gene_synonym="ARHGAP7; HP; p122-RhoGAP; STARD12"
                     /note="major polyA site"
ORIGIN      
        1 agtctgaatt tagtagacag aagtcactag gaatgccttg acaggatcct gccttagcta
       61 aggctccctc cagctgcaga gggtgttttt gttagactca cacactgcgt gaaactgctc
      121 agaatagagc catgatctca accacgaaat gggaacttag attttggaga aactaacggg
      181 gacggacttc tttcctagcc tgagtgttga gcagtgtcat gccttggcgt ttcagctcct
      241 cgttgtctag gtggtgaaat gacagaactc attcgcttct ttgattggtg attttgaaat
      301 aatctttcat caagttccat ctcctttacc ctcatatgga atatatctct ctgtctgttg
      361 ttaaactacg atgacatgtc tgtagctatc agaaagagaa gctgggaaga acatgtgacc
      421 cactggatgg gacagccttt taattctgat gatcgtaaca cagcatgtca tcatggacta
      481 gtagctgaca gcttgcaggc aagtatggaa aaagatgcaa ctctaaatgt ggaccgcaaa
      541 gagaagtgtg tttcactacc tgactgctgt catggatcag agctgagaga ttttcctggg
      601 aggccaatgg gtcatctttc aaaggatgtg gacgaaaatg acagccatga aggtgaagat
      661 cagtttcttt ctctggaagc cagcacagaa acactagtgc atgtttctga tgaggataac
      721 aatgctgatt tatgccttac agatgataaa caggttttaa atacccaagg gcagaaaaca
      781 tcaggccaac atatgatcca aggagcaggc tccttagaaa aggcactgcc catcatacaa
      841 agtaaccaag tttcttctaa ctcctgggga atagctggtg aaactgaatt agcactggta
      901 aaagaaagtg gggagagaaa agttactgac tctataagta aaagcctgga gctttgcaat
      961 gaaataagct taagtgaaat aaaagatgca cccaaagtaa atgcagtgga tactttgaac
     1021 gtgaaagata ttgcacctga gaaacaattg cttaactctg ctgtaattgc tcagcaacga
     1081 aggaaacctg acccccctaa agatgaaaat gaaagaagca cctgcaatgt agtacaaaat
     1141 gagttcttgg atactccttg cacaaacaga ggactgccat tattaaaaac agattttgga
     1201 agctgccttc tgcagcctcc ttcctgcccc aatggaatgt cagctgaaaa tggcctggag
     1261 aagagtggtt tttcacaaca tcaaaacaaa agtccaccaa aggtcaaggc agaagatggc
     1321 atgcagtgtt tacaattaaa ggagaccctg gccacccagg aacccacaga taaccaagtc
     1381 agacttcgta agagaaagga aataagagaa gatcgagata gggcgcggct ggactccatg
     1441 gtgctgctga ttatgaaact ggaccagctt gatcaggaca tagaaaatgc cctcagcacc
     1501 agctcctctc catcaggcac accaacaaac ctgcggcggc acgttcctga tctggaatca
     1561 ggatctgaaa gtggagcaga taccatttca gtaaatcaga cacgagtaaa tttgtcttct
     1621 gacactgagt ccacggacct cccatcttcc actccagtag ccaattctgg aaccaaaccc
     1681 aagactacgg ctattcaagg tatttcagag aaggaaaagg ctgaaattga agccaaggaa
     1741 gcttgtgatt ggctacgggc aactggtttc ccccagtatg cacagcttta tgaagatttc
     1801 ctgttcccca tcgatatttc cttggtcaag agagagcatg attttttgga cagagatgcc
     1861 attgaggctc tatgcaggcg tctaaatact ttaaacaaat gtgcggtgat gaagctagaa
     1921 attagtcctc atcggaaacg aagtgacgat tcagacgagg atgagccttg tgccatcagt
     1981 ggcaaatgga ctttccaaag ggacagcaag aggtggtccc ggcttgaaga gtttgatgtc
     2041 ttttctccaa aacaagacct ggtccctggg tccccagacg actcccaccc gaaggacggc
     2101 cccagccccg gaggcacgct gatggacctc agcgagcgcc aggaggtgtc ttccgtccgc
     2161 agcctcagca gcactggcag cctccccagc cacgcgcccc ccagcgagga tgctgccacc
     2221 ccccggacta actccgtcat cagcgtttgc tcctccagca acttggcagg caatgacgac
     2281 tctttcggca gcctgccctc tcccaaggaa ctgtccagct tcagcttcag catgaaaggc
     2341 cacgaaaaaa ctgccaagtc caagacgcgc agtctgctga aacggatgga gagcctgaag
     2401 ctcaagagct cccatcacag caagcacaaa gcgccctcaa agctggggtt gatcatcagc
     2461 gggcccatct tgcaagaggg gatggatgag gagaagctga agcagctcaa ctgcgtggag
     2521 atctccgccc tcaatggcaa ccgcatcaac gtccccatgg tacgaaagag gagcgtttcc
     2581 aactccacgc agaccagcag cagcagcagc cagtcggaga ccagcagcgc ggtcagcacg
     2641 cccagccctg ttacgaggac ccggagcctc agtgcgtgca acaagcgggt gggcatgtac
     2701 ttagagggct tcgatccttt caatcagtca acatttaaca acgtggtgga gcagaacttt
     2761 aagaaccgcg agagctaccc agaggacacg gtgttctaca tccctgaaga tcacaagcct
     2821 ggcactttcc ccaaagctct caccaatggc agtttctccc cctcggggaa taacggctct
     2881 gtgaactgga ggacgggaag cttccacggc cctggccaca tcagcctcag gagggaaaac
     2941 agtagcgaca gccccaagga actgaagaga cgcaattctt ccagctccat gagcagccgc
     3001 ctgagcatct acgacaacgt gccgggctcc atcctctact ccagttcagg ggacctggcg
     3061 gatctggaga acgaggacat cttccccgag ctggacgaca tcctctacca cgtgaagggg
     3121 atgcagcgga tagtcaatca gtggtcggag aagttttctg atgagggaga ttcggactca
     3181 gccctggact cggtctctcc ctgcccgtcc tctccaaaac agatacacct ggatgtggac
     3241 aacgaccgaa ccacacccag cgacctggac agcacaggca actccctgaa tgaaccggaa
     3301 gagccctccg agatcccgga aagaagggat tctggggttg gggcttccct aaccaggtcc
     3361 aacaggcacc gactgagatg gcacagtttc cagagctcac atcggccaag cctcaactct
     3421 gtatcactac agattaactg ccagtctgtg gcccagatga acctgctgca gaaatactca
     3481 ctcctaaagc taacggccct gctggagaaa tacacacctt ctaacaagca tggttttagc
     3541 tgggccgtgc ccaagttcat gaagaggatc aaggttccag actacaagga ccggagtgtg
     3601 tttggggtcc cactgacggt caacgtgcag cgcacaggac aaccgttgcc tcagagcatc
     3661 cagcaggcca tgcgatacct ccggaaccat tgtttggatc aggttgggct cttcagaaaa
     3721 tcgggggtca agtcccggat tcaggctctg cgccagatga atgaaggtgc catagactgt
     3781 gtcaactacg aaggacagtc tgcttatgac gtggcagaca tgctgaagca gtattttcga
     3841 gatcttcctg agccactaat gacgaacaaa ctctcggaaa cctttctaca gatctaccaa
     3901 tatgtgccca aggaccagcg cctgcaggcc atcaaggctg ccatcatgct gctgcctgac
     3961 gagaaccggg aggttctgca gaccctgctt tatttcctga gcgatgtcac agcagccgta
     4021 aaagaaaacc agatgacccc aaccaacctg gccgtgtgct tagcgccttc cctcttccat
     4081 ctcaacaccc tgaagagaga gaattcctct cccagggtaa tgcaaagaaa acaaagtttg
     4141 ggcaaaccag atcagaaaga tttgaatgaa aacctagctg ccactcaagg gctggcccat
     4201 atgatcgccg agtgcaagaa gcttttccag gttcccgagg aaatgagccg atgtcgtaat
     4261 tcctataccg aacaagagct gaagcccctc actctggaag cactcgggca cctgggtaat
     4321 gatgactcag ctgactacca acacttcctc caggactgtg tggatggcct gtttaaagaa
     4381 gtcaaagaga agtttaaagg ctgggtcagc tactccactt cggagcaggc tgagctgtcc
     4441 tataagaagg tgagcgaagg accccctctg aggctttgga ggtcagtcat tgaagtccct
     4501 gctgtgccag aggaaatctt aaagcgccta cttaaagaac agcacctctg ggatgtagac
     4561 ctgttggatt caaaagtgat cgaaattctg gacagccaaa ctgaaattta ccagtatgtc
     4621 caaaacagta tggcacctca tcctgctcga gactacgttg ttttaagaac ctggaggact
     4681 aatttaccca aaggagcctg tgccctttta ctaacctctg tggatcacga tcgcgcacct
     4741 gtggtgggtg tgagggttaa tgtgctcttg tccaggtatt tgattgaacc ctgtgggcca
     4801 ggaaaatcca aactcaccta catgtgcaga gttgacttaa ggggccacat gccagaatgg
     4861 tacacaaaat cttttggaca tttgtgtgca gctgaagttg taaagatccg ggattccttc
     4921 agtaaccaga acactgaaac caaagacacc aaatctaggt gatcactgaa gcaacgcaac
     4981 cgcttccacc accatggtgt ttgtttctag aacttttgcc agtccttgaa gaatgggttc
     5041 tgtgtctaat cctgaaacaa agaaaactac aagctggagt gtaggaattg actatagcaa
     5101 tttgatacat ttttaaagct gcttcctgtt tgttgagggt ctgtattcat agaccttgac
     5161 tggaatatgt aagactgtgc aaaaaaaaaa aaaaaattta tgtgtattct tattcaaatt
     5221 gcttctgaga agcaaaactc tttaaataca ttatggaaga taatgaagat acttcattct
     5281 cttgtgatat cagtgtatgc gtacctgtgt cgctttattt gcagtgtgtt gagggactgg
     5341 tgtatccact ggaatagttg gtactcttgg atgtgttttc tcaccaagat gagcaaagaa
     5401 aggtttgcac agaggagtgt gaatgtgtgt ttgttgctgg ctgaatggca atagatgtct
     5461 aaggtggatt cagtgtctgg cacactgaga cacctccaag aaggagattg atgcatcagg
     5521 ttcagtttaa cctggaatat ctgactaccc ctgaatccac ccagaaaggg ggcccaacac
     5581 ccttgtccat ttatgggtat tttttttcga agttattaag catattcctt ttccacgaac
     5641 ctcttctgta ctttgattgt aataggttgg ctcttacacc cattccaaat gcagtttatt
     5701 tttagacccg attgcaaata gtgatgtagt tttaaccagt atggattagt tcagggatga
     5761 actgctccct ctagccttac tggctctgat ccacagggtt ttgttttgtt ttgttttgtt
     5821 ttttgtttaa gtcgagatat aaaaactgaa cacgataaca cttactctta aatcaagcat
     5881 caacactttt tccctgttag aattctttgc atttttgtgt ttgtaacaga aacgccttaa
     5941 gacactatgt ttgggaatat aggaaactat gtgtgtccca aggaaatccc tgtaaattta
     6001 actcacctac aaaaggcttt ttccccgcct ttggttgtta acggcattcc tgaaagccac
     6061 atgtgtttat tcattgggct tgttcttatc agcaaatagg ttttctggtt ttatgacttt
     6121 ttgtcttatt ttatttttcc tacatttctt tttttttttt tttcctttag aatgccctgg
     6181 aaatatattt aagtggtaat gaaaaatagt aatcatagta aaacgcaaca agaagaaaac
     6241 caacccaaac cagtgaagtt ttttagaacc tttagaaggg tggtctttat tcaggtttta
     6301 ctgtaatggt aaggattgac tcaagagaca gtattagtaa atttattgtg tatggatcaa
     6361 aagtgaataa tgtatgaatg agagctgtaa gaaggatttt tattttgtta taatttagtt
     6421 accattttca gtgttatttc aaaggttctt tgaagaattt tggggcaggg catcagatta
     6481 gagttttaaa atttgagtat tttggatatc agtgttcctc atgaagatat acatggatat
     6541 tcaattttga tggcttccag atttgtaaga ttgtatgttg tatataccat tctattaaga
     6601 aacatgtcca ctgtgctttc aaacatagat aaagcatgat aaagattatt atttaagata
     6661 tacttgtatt tatacctcag atattctttt gggttttgta cctcaaggct tttttcttct
     6721 tattgtaaat acactttacg tgaatacagt ctaagtgaag aaaataaata aaaggaagag
     6781 gtttataact tgctctatat ctgtacagat tataatcaat aagtgcacta ttattaaatg
     6841 tttaaagtaa gggaaaagtc tgggctgcct tccttaatat tgcatctcac tcccaccctt
     6901 aaaaccacag attgcaaagc atagcatttt agcatcaact acaatcaaaa gagcgatttg
     6961 ctgaaggaaa aatcggactg caaatcattc caaggccaaa ctgcaactga gccacccact
     7021 cccaaacagg aaaccctggt gaaggttcag gaagcacgga gattctctcc aacaaaggtc
     7081 cagttaggaa acgacgctga gaggatgacg acaacgtgca acagcagaaa gatgcttgca
     7141 agcagagtca gggtcaccag tgaatgccac aaaagttctc tttcccactg tttaatttga
     7201 caagagaaga atttgaagga tatgaacatt ttcaagaact ctgctgaggt cacttagagc
     7261 gccatcacaa cttatttgtg tgactaattg cctagattgt aagctctttg agggcagggc
     7321 ttgtctctta cacatcttta taatcccctg cagcggcttt cagtattttg tacttgtagg
     7381 cacctaataa atttattatt tgctatactg aa
//
