LOCUS       NM_001931               3878 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens dihydrolipoamide S-acetyltransferase (DLAT),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001931
VERSION     NM_001931.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,X., Sun,Y., Li,C., Wang,M., Zang,Q., Zhang,X., Wang,F., Niu,Y.
            and Hua,J.
  TITLE     Novel Insights Into DLAT's Role in Alzheimer's Disease-Related
            Copper Toxicity Through Microglial Exosome Dynamics
  JOURNAL   CNS Neurosci Ther 30 (10), e70064 (2024)
   PUBMED   39428563
  REMARK    GeneRIF: Novel Insights Into DLAT's Role in Alzheimer's
            Disease-Related Copper Toxicity Through Microglial Exosome
            Dynamics.
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Zhang,P., Zhao,J.H., Yuan,L.X., Ju,L.L., Wang,H.X., Wang,F.,
            Chen,L. and Cai,W.H.
  TITLE     DLAT is a promising prognostic marker and therapeutic target for
            hepatocellular carcinoma: a comprehensive study based on public
            databases
  JOURNAL   Sci Rep 13 (1), 17295 (2023)
   PUBMED   37828099
  REMARK    GeneRIF: DLAT is a promising prognostic marker and therapeutic
            target for hepatocellular carcinoma: a comprehensive study based on
            public databases.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3878)
  AUTHORS   Xu,L., Wu,P., Rong,A., Li,K., Xiao,X., Zhang,Y. and Wu,H.
  TITLE     Systematic pan-cancer analysis identifies cuproptosis-related gene
            DLAT as an immunological and prognostic biomarker
  JOURNAL   Aging (Albany NY) 15 (10), 4269-4287 (2023)
   PUBMED   37199628
  REMARK    GeneRIF: Systematic pan-cancer analysis identifies
            cuproptosis-related gene DLAT as an immunological and prognostic
            biomarker.
REFERENCE   4  (bases 1 to 3878)
  AUTHORS   Yang,Q., Zeng,S. and Liu,W.
  TITLE     Roles of cuproptosis-related gene DLAT in various cancers: a
            bioinformatic analysis and preliminary verification on pro-survival
            autophagy
  JOURNAL   PeerJ 11, e15019 (2023)
   PUBMED   36949759
  REMARK    GeneRIF: Roles of cuproptosis-related gene DLAT in various cancers:
            a bioinformatic analysis and preliminary verification on
            pro-survival autophagy.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3878)
  AUTHORS   Fang,Z., Wang,W., Liu,Y., Hua,J., Liang,C., Liu,J., Zhang,B.,
            Shi,S., Yu,X., Meng,Q. and Xu,J.
  TITLE     Cuproptosis-Related Gene DLAT as a Novel Biomarker Correlated with
            Prognosis, Chemoresistance, and Immune Infiltration in Pancreatic
            Adenocarcinoma: A Preliminary Study Based on Bioinformatics
            Analysis
  JOURNAL   Curr Oncol 30 (3), 2997-3019 (2023)
   PUBMED   36975441
  REMARK    GeneRIF: Cuproptosis-Related Gene DLAT as a Novel Biomarker
            Correlated with Prognosis, Chemoresistance, and Immune Infiltration
            in Pancreatic Adenocarcinoma: A Preliminary Study Based on
            Bioinformatics Analysis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3878)
  AUTHORS   Ganetzky,R., McCormick,E.M. and Falk,M.J.
  TITLE     Primary Pyruvate Dehydrogenase Complex Deficiency Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   34138529
REFERENCE   7  (bases 1 to 3878)
  AUTHORS   Moehario,L.H., Smooker,P.M., Devenish,R.J., Mackay,I.R.,
            Gershwin,M.E. and Marzuki,S.
  TITLE     Nucleotide sequence of a cDNA encoding the lipoate acetyl
            transferase (E2) of human heart pyruvate dehydrogenase complex
            differs from that of human placenta
  JOURNAL   Biochem Int 20 (2), 417-422 (1990)
   PUBMED   2317220
REFERENCE   8  (bases 1 to 3878)
  AUTHORS   Thekkumkara,T.J., Ho,L., Wexler,I.D., Pons,G., Liu,T.C. and
            Patel,M.S.
  TITLE     Nucleotide sequence of a cDNA for the dihydrolipoamide
            acetyltransferase component of human pyruvate dehydrogenase complex
  JOURNAL   FEBS Lett 240 (1-2), 45-48 (1988)
   PUBMED   3191998
REFERENCE   9  (bases 1 to 3878)
  AUTHORS   Coppel,R.L., McNeilage,L.J., Surh,C.D., Van de Water,J.,
            Spithill,T.W., Whittingham,S. and Gershwin,M.E.
  TITLE     Primary structure of the human M2 mitochondrial autoantigen of
            primary biliary cirrhosis: dihydrolipoamide acetyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 85 (19), 7317-7321 (1988)
   PUBMED   3174635
REFERENCE   10 (bases 1 to 3878)
  AUTHORS   Thekkumkara,T.J., Jesse,B.W., Ho,L., Raefsky,C., Pepin,R.A.,
            Javed,A.A., Pons,G. and Patel,M.S.
  TITLE     Isolation of a cDNA clone for the dihydrolipoamide
            acetyltransferase component of the human liver pyruvate
            dehydrogenase complex
  JOURNAL   Biochem Biophys Res Commun 145 (2), 903-907 (1987)
   PUBMED   3036145
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000907.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 2, 2019 this sequence version replaced NM_001931.4.
            
            Summary: This gene encodes component E2 of the multi-enzyme
            pyruvate dehydrogenase complex (PDC). PDC resides in the inner
            mitochondrial membrane and catalyzes the conversion of pyruvate to
            acetyl coenzyme A. The protein product of this gene,
            dihydrolipoamide acetyltransferase, accepts acetyl groups formed by
            the oxidative decarboxylation of pyruvate and transfers them to
            coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for
            antimitochondrial antibodies. These autoantibodies are present in
            nearly 95% of patients with the autoimmune liver disease primary
            biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and
            destroy epithelial cells in the bile duct where this protein is
            abnormally distributed and overexpressed. PBC enventually leads to
            cirrhosis and liver failure. Mutations in this gene are also a
            cause of pyruvate dehydrogenase E2 deficiency which causes primary
            lactic acidosis in infancy and early childhood.[provided by RefSeq,
            Oct 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039084.1, AL832703.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000280346.11/
                                                  ENSP00000280346.7
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-344               AP000907.6         127025-127368
            345-446             AP000907.6         127815-127916
            447-571             AP000907.6         130132-130256
            572-725             AP000907.6         130409-130562
            726-852             AP000907.6         135021-135147
            853-1040            AP000907.6         138890-139077
            1041-1194           AP000907.6         140861-141014
            1195-1262           AP000907.6         145083-145150
            1263-1355           AP000907.6         146755-146847
            1356-1463           AP000907.6         147480-147587
            1464-1579           AP000907.6         152851-152966
            1580-1742           AP000907.6         161520-161682
            1743-1879           AP000907.6         162655-162791
            1880-3878           AP000907.6         164023-166021
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.1"
     gene            1..3878
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="dihydrolipoamide S-acetyltransferase"
                     /db_xref="GeneID:1737"
                     /db_xref="HGNC:HGNC:2896"
                     /db_xref="MIM:608770"
     exon            1..344
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..2009
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /EC_number="2.3.1.12"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; E2 component of pyruvate dehydrogenase complex;
                     dihydrolipoyllysine-residue acetyltransferase component of
                     pyruvate dehydrogenase complex, mitochondrial; 70 kDa
                     mitochondrial autoantigen of primary biliary cirrhosis; M2
                     antigen complex 70 kDa subunit; pyruvate dehydrogenase
                     complex component E2; dihydrolipoamide acetyltransferase
                     component of pyruvate dehydrogenase complex"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex, mitochondrial
                     isoform 2 precursor"
                     /protein_id="NP_001922.2"
                     /db_xref="CCDS:CCDS8354.1"
                     /db_xref="GeneID:1737"
                     /db_xref="HGNC:HGNC:2896"
                     /db_xref="MIM:608770"
                     /translation="MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPAR
                     RNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPT
                     MQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIG
                     AIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPH
                     MQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKIL
                     VPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQ
                     PLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSF
                     VPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSI
                     DVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHV
                     VDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTIS
                     NLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVD
                     GAVGAQWLAEFRKYLEKPITMLL"
     exon            345..446
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            447..571
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            572..725
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..852
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1040
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1194
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1262
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1355
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1463
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1579
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1742
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1879
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..3878
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2763
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="major polyA site"
     regulatory      3063..3068
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: AGTAAA"
     polyA_site      3090
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="major polyA site"
     regulatory      3322..3327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: AATAAA"
     polyA_site      3339
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
     regulatory      3706..3711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: AATAAA"
     regulatory      3726..3731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: AATAAA"
     polyA_site      3731
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
     polyA_site      3752
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
     regulatory      3845..3850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: ATTAAA"
     regulatory      3852..3857
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
                     /note="hexamer: TATAAA"
     polyA_site      3864
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
     polyA_site      3878
                     /gene="DLAT"
                     /gene_synonym="DLTA; E2; PBC; PDC-E2; PDCE2"
ORIGIN      
        1 gctgctcttg gagaggtcac tccggagacg gcgttggttt tggggtgtgg ggggttggtg
       61 gcactatgtg gcgcgtctgt gcgcgacggg ctcagaatgt agccccatgg gcgggactcg
      121 aggctcggtg gacggccttg caggaggtac ccggaactcc acgagtgacc tcgcgatctg
      181 gcccggctcc cgctcgtcgc aacagcgtga ctacagggta tggcggggtc cgggcactgt
      241 gcggctggac ccccagttct ggggccacgc cgcggaaccg cttactgctg cagcttttgg
      301 ggtcgcccgg ccgccgctat tacagtcttc ccccgcatca gaaggttcca ttgccttctc
      361 tttcccccac aatgcaggca ggcaccatag cccgttggga aaaaaaagag ggggacaaaa
      421 tcaatgaagg tgacctaatt gcagaggttg aaactgataa agccactgtt ggatttgaga
      481 gcctggagga gtgttatatg gcaaagatac ttgttgctga aggtaccagg gatgttccca
      541 tcggagcgat catctgtatc acagttggca agcctgagga tattgaggcc tttaaaaatt
      601 atacactgga ttcctcagca gcacctaccc cacaagcggc cccagcacca acccctgctg
      661 ccactgcttc gccacctaca ccttctgctc aggctcctgg tagctcatat ccccctcaca
      721 tgcaggtact tcttcctgcc ctctctccca ccatgaccat gggcacagtt cagagatggg
      781 aaaaaaaagt gggtgagaag ctaagtgaag gagacttact ggcagagata gaaactgaca
      841 aagccactat aggttttgaa gtacaggaag aaggttatct ggcaaaaatc ctggtccctg
      901 aaggcacaag agatgtccct ctaggaaccc cactctgtat cattgtagaa aaagaggcag
      961 atatatcagc atttgctgac tataggccaa ccgaagtaac agatttaaaa ccacaagtgc
     1021 caccacctac cccacccccg gtggccgctg ttcctccaac tccccagcct ttagctccta
     1081 caccttcagc accctgccca gctactcctg ctggaccaaa gggaagggtg tttgttagcc
     1141 ctcttgcaaa gaagttggca gtagagaaag ggattgatct tacacaagta aaagggacag
     1201 gaccagatgg tagaatcacc aagaaggata tcgactcttt tgtgcctagt aaagttgctc
     1261 ctgctccggc agctgttgtg cctcccacag gtcctggaat ggcaccagtt cctacaggtg
     1321 tcttcacaga tatcccaatc agcaacattc gtcgggttat tgcacagcga ttaatgcaat
     1381 caaagcaaac catacctcat tattaccttt ctatcgatgt aaatatggga gaagttttgt
     1441 tggtacggaa agaacttaat aagatattag aagggagaag caaaatttct gtcaatgact
     1501 tcatcataaa agcttcagct ttggcatgtt taaaagttcc cgaagcaaat tcttcttgga
     1561 tggacacagt tataagacaa aatcatgttg ttgatgtcag tgttgcggtc agtactcctg
     1621 caggactcat cacacctatt gtgtttaatg cacatataaa aggagtggaa accattgcta
     1681 atgatgttgt ttctttagca accaaagcaa gagagggtaa actacagcca catgaattcc
     1741 agggtggcac ttttacgatc tccaatttag gaatgtttgg aattaagaat ttctctgcta
     1801 ttattaaccc acctcaagca tgtattttgg caattggtgc ttcagaggat aaactggtcc
     1861 ctgcagataa tgaaaaaggg tttgatgtgg ctagcatgat gtctgttaca ctcagttgtg
     1921 atcaccgggt ggtggatgga gcagttggag cccagtggct tgctgagttt agaaagtacc
     1981 ttgaaaaacc tatcactatg ttgttgtaac taactcaaga atttctaaac tctcccaggt
     2041 cacactgatt cattcttaac aagatattta tatgttatta aacaggtggt tctttttatt
     2101 ttaaccagtt atttttatta ttgagtctgt ccagataagt tatttataat gggcattact
     2161 gaatttttaa aatgccgatt acacccaaat attgtgcaca tttaataatc agacaccaga
     2221 tttttagctc tgtactccta attaagggac atgtatgtgg ccttgcctag ccctttggtg
     2281 ataagtactt cctctaggaa atgtacgata ggtagaattg tggttcccta aagacaagta
     2341 cataaaggtg accctgatga aaccttgaag ttctgaaatt taactgccta aaatgttctc
     2401 cttagatgtg agagaaagag aaatcagaaa aattaattct cttgggggaa gggcttgaat
     2461 tgaagcttta ctttagaatt tagccctggt ttgaaatttt ccattacatg atcttggttt
     2521 atcatcgatg ggaagggtag aaaacttcaa ggaaaataag tgaaatttta aaagtcagca
     2581 ttttcttaga cctcttcagc tgattgttta tttttctatg aattcctaca catggttatt
     2641 cccccctact tgagataatc taaatataaa ccagctactt gatgtaactg agaatttgtg
     2701 tggatattta tttaaacaaa tgtgtaattt tgagtacaga attcaacagt tacctccaaa
     2761 aaagaaacat tgttaatata atttaacaga agttgtgaaa ctaaaatttt ctaagattaa
     2821 ctggtagttc attgtaaatg aacataatga acagaattta tgactccact gtggaaaatg
     2881 ctatcaaata actaaggaat atatatggaa taagtgtaca tatgtaaaat attgttacta
     2941 gagttagata tgtgccaaag tccatttatc ccaaatcctg tctgaaaagg aggggtacat
     3001 tggtaaacat tttggagtgc ttaaaaatgc caaaaacaaa atggtaattt ctactttgat
     3061 aaagtaaaaa agttaaatgt gtgtaaaaaa gtgttctgtg tccttctact ccagcatcgt
     3121 ctcatgtaaa ataagaaagc cctaaaatac tattggagag aaaaaattaa ctaggttgct
     3181 actttatttg cctaaatact tttttctatt ttgttagatt ttgcctttct tttggaagga
     3241 aggaggcgat attctggatt ataaaaatga attgggaaca ttatcacaat tccagacttt
     3301 ctattaatat ttatgtgttt taataaacgt ttgaaattat ttatgactac ttaaaatgaa
     3361 tctgaccagt gcttcctggt atatgtaata tgtggagtta gcccctgaaa tttgctttaa
     3421 gtgtttcagt gttgaatctg tttctaaata ttcattatta catggtacac aagtgacact
     3481 ccatatattc cacacagact tttaccttgc tgtattatta tgaaaacaat acattaattt
     3541 gatttttcag taattagtaa ttttaggttg aagattatcc aaaagaaaca agcttatcac
     3601 aagggaccat catcaatatg atccaaatct ggttcaaaca ttcaaaactt caaagataat
     3661 tcatctttcg ctaatgcttg tggttctgtt gttcccttga aaaaaaataa aaacagttgc
     3721 cttctaataa acattgttga gttaaaaatt aatctgagct ataatctaaa tcgattcagt
     3781 ctcttaccag aactgaaaga aaaaagttag aaatagtgtt tttggttcat atgattattt
     3841 aaaaattaaa gtataaatct tcaatatgtc tggtttaa
//
