LOCUS       NM_152383               3366 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript
            variant 1, mRNA.
ACCESSION   NM_152383
VERSION     NM_152383.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Garcia-Moreno,J.F., Lacerda,R., da Costa,P.J., Pereira,M.,
            Gama-Carvalho,M., Matos,P. and Romao,L.
  TITLE     DIS3L2 knockdown impairs key oncogenic properties of colorectal
            cancer cells via the mTOR signaling pathway
  JOURNAL   Cell Mol Life Sci 80 (7), 185 (2023)
   PUBMED   37340282
  REMARK    GeneRIF: DIS3L2 knockdown impairs key oncogenic properties of
            colorectal cancer cells via the mTOR signaling pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Roithova,A., Feketova,Z., Vanacova,S. and Stanek,D.
  TITLE     DIS3L2 and LSm proteins are involved in the surveillance of Sm
            ring-deficient snRNAs
  JOURNAL   Nucleic Acids Res 48 (11), 6184-6197 (2020)
   PUBMED   32374871
  REMARK    GeneRIF: DIS3L2 and LSm proteins are involved in the surveillance
            of Sm ring-deficient snRNAs.
            Erratum:[Nucleic Acids Res. 2022 Nov 28;50(21):12597. doi:
            10.1093/nar/gkac1154.. PMID: 36416267]
REFERENCE   3  (bases 1 to 3366)
  AUTHORS   Yang,A., Shao,T.J., Bofill-De Ros,X., Lian,C., Villanueva,P.,
            Dai,L. and Gu,S.
  TITLE     AGO-bound mature miRNAs are oligouridylated by TUTs and
            subsequently degraded by DIS3L2
  JOURNAL   Nat Commun 11 (1), 2765 (2020)
   PUBMED   32488030
  REMARK    GeneRIF: AGO-bound mature miRNAs are oligouridylated by TUTs and
            subsequently degraded by DIS3L2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3366)
  AUTHORS   Pirouz,M., Wang,C.H., Liu,Q., Ebrahimi,A.G., Shamsi,F., Tseng,Y.H.
            and Gregory,R.I.
  TITLE     The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic
            reticulum-targeted mRNA translation and calcium ion homeostasis
  JOURNAL   Nat Commun 11 (1), 2619 (2020)
   PUBMED   32457326
  REMARK    GeneRIF: The Perlman syndrome DIS3L2 exoribonuclease safeguards
            endoplasmic reticulum-targeted mRNA translation and calcium ion
            homeostasis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3366)
  AUTHORS   da Costa,P.J., Menezes,J., Saramago,M., Garcia-Moreno,J.F.,
            Santos,H.A., Gama-Carvalho,M., Arraiano,C.M., Viegas,S.C. and
            Romao,L.
  TITLE     A role for DIS3L2 over natural nonsense-mediated mRNA decay targets
            in human cells
  JOURNAL   Biochem Biophys Res Commun 518 (4), 664-671 (2019)
   PUBMED   31466720
  REMARK    GeneRIF: Findings indicate a role for DIS3 like 3'-5'
            exoribonuclease 2 (DIS3L2) and uridylation in nonsense-mediated
            mRNA decay (NMD).
REFERENCE   6  (bases 1 to 3366)
  AUTHORS   Malecki,M., Viegas,S.C., Carneiro,T., Golik,P., Dressaire,C.,
            Ferreira,M.G. and Arraiano,C.M.
  TITLE     The exoribonuclease Dis3L2 defines a novel eukaryotic RNA
            degradation pathway
  JOURNAL   EMBO J 32 (13), 1842-1854 (2013)
   PUBMED   23503588
REFERENCE   7  (bases 1 to 3366)
  AUTHORS   Morris,M.R., Astuti,D. and Maher,E.R.
  TITLE     Perlman syndrome: overgrowth, Wilms tumor predisposition and DIS3L2
  JOURNAL   Am J Med Genet C Semin Med Genet 163C (2), 106-113 (2013)
   PUBMED   23613427
  REMARK    GeneRIF: in cellular models DIS3L2 knockdown is associated with
            abnormalities of cell growth and division
REFERENCE   8  (bases 1 to 3366)
  AUTHORS   Berndt,S.I., Gustafsson,S., Magi,R., Ganna,A., Wheeler,E.,
            Feitosa,M.F., Justice,A.E., Monda,K.L., Croteau-Chonka,D.C.,
            Day,F.R., Esko,T., Fall,T., Ferreira,T., Gentilini,D.,
            Jackson,A.U., Luan,J., Randall,J.C., Vedantam,S., Willer,C.J.,
            Winkler,T.W., Wood,A.R., Workalemahu,T., Hu,Y.J., Lee,S.H.,
            Liang,L., Lin,D.Y., Min,J.L., Neale,B.M., Thorleifsson,G., Yang,J.,
            Albrecht,E., Amin,N., Bragg-Gresham,J.L., Cadby,G., den Heijer,M.,
            Eklund,N., Fischer,K., Goel,A., Hottenga,J.J., Huffman,J.E.,
            Jarick,I., Johansson,A., Johnson,T., Kanoni,S., Kleber,M.E.,
            Konig,I.R., Kristiansson,K., Kutalik,Z., Lamina,C., Lecoeur,C.,
            Li,G., Mangino,M., McArdle,W.L., Medina-Gomez,C.,
            Muller-Nurasyid,M., Ngwa,J.S., Nolte,I.M., Paternoster,L.,
            Pechlivanis,S., Perola,M., Peters,M.J., Preuss,M., Rose,L.M.,
            Shi,J., Shungin,D., Smith,A.V., Strawbridge,R.J., Surakka,I.,
            Teumer,A., Trip,M.D., Tyrer,J., Van Vliet-Ostaptchouk,J.V.,
            Vandenput,L., Waite,L.L., Zhao,J.H., Absher,D., Asselbergs,F.W.,
            Atalay,M., Attwood,A.P., Balmforth,A.J., Basart,H., Beilby,J.,
            Bonnycastle,L.L., Brambilla,P., Bruinenberg,M., Campbell,H.,
            Chasman,D.I., Chines,P.S., Collins,F.S., Connell,J.M.,
            Cookson,W.O., de Faire,U., de Vegt,F., Dei,M., Dimitriou,M.,
            Edkins,S., Estrada,K., Evans,D.M., Farrall,M., Ferrario,M.M.,
            Ferrieres,J., Franke,L., Frau,F., Gejman,P.V., Grallert,H.,
            Gronberg,H., Gudnason,V., Hall,A.S., Hall,P., Hartikainen,A.L.,
            Hayward,C., Heard-Costa,N.L., Heath,A.C., Hebebrand,J., Homuth,G.,
            Hu,F.B., Hunt,S.E., Hypponen,E., Iribarren,C., Jacobs,K.B.,
            Jansson,J.O., Jula,A., Kahonen,M., Kathiresan,S., Kee,F.,
            Khaw,K.T., Kivimaki,M., Koenig,W., Kraja,A.T., Kumari,M.,
            Kuulasmaa,K., Kuusisto,J., Laitinen,J.H., Lakka,T.A.,
            Langenberg,C., Launer,L.J., Lind,L., Lindstrom,J., Liu,J.,
            Liuzzi,A., Lokki,M.L., Lorentzon,M., Madden,P.A., Magnusson,P.K.,
            Manunta,P., Marek,D., Marz,W., Mateo Leach,I., McKnight,B.,
            Medland,S.E., Mihailov,E., Milani,L., Montgomery,G.W., Mooser,V.,
            Muhleisen,T.W., Munroe,P.B., Musk,A.W., Narisu,N., Navis,G.,
            Nicholson,G., Nohr,E.A., Ong,K.K., Oostra,B.A., Palmer,C.N.,
            Palotie,A., Peden,J.F., Pedersen,N., Peters,A., Polasek,O.,
            Pouta,A., Pramstaller,P.P., Prokopenko,I., Putter,C.,
            Radhakrishnan,A., Raitakari,O., Rendon,A., Rivadeneira,F.,
            Rudan,I., Saaristo,T.E., Sambrook,J.G., Sanders,A.R., Sanna,S.,
            Saramies,J., Schipf,S., Schreiber,S., Schunkert,H., Shin,S.Y.,
            Signorini,S., Sinisalo,J., Skrobek,B., Soranzo,N., Stancakova,A.,
            Stark,K., Stephens,J.C., Stirrups,K., Stolk,R.P., Stumvoll,M.,
            Swift,A.J., Theodoraki,E.V., Thorand,B., Tregouet,D.A., Tremoli,E.,
            Van der Klauw,M.M., van Meurs,J.B., Vermeulen,S.H., Viikari,J.,
            Virtamo,J., Vitart,V., Waeber,G., Wang,Z., Widen,E., Wild,S.H.,
            Willemsen,G., Winkelmann,B.R., Witteman,J.C., Wolffenbuttel,B.H.,
            Wong,A., Wright,A.F., Zillikens,M.C., Amouyel,P., Boehm,B.O.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Chanock,S.J.,
            Cupples,L.A., Cusi,D., Dedoussis,G.V., Erdmann,J., Eriksson,J.G.,
            Franks,P.W., Froguel,P., Gieger,C., Gyllensten,U., Hamsten,A.,
            Harris,T.B., Hengstenberg,C., Hicks,A.A., Hingorani,A., Hinney,A.,
            Hofman,A., Hovingh,K.G., Hveem,K., Illig,T., Jarvelin,M.R.,
            Jockel,K.H., Keinanen-Kiukaanniemi,S.M., Kiemeney,L.A., Kuh,D.,
            Laakso,M., Lehtimaki,T., Levinson,D.F., Martin,N.G., Metspalu,A.,
            Morris,A.D., Nieminen,M.S., Njolstad,I., Ohlsson,C.,
            Oldehinkel,A.J., Ouwehand,W.H., Palmer,L.J., Penninx,B., Power,C.,
            Province,M.A., Psaty,B.M., Qi,L., Rauramaa,R., Ridker,P.M.,
            Ripatti,S., Salomaa,V., Samani,N.J., Snieder,H., Sorensen,T.I.,
            Spector,T.D., Stefansson,K., Tonjes,A., Tuomilehto,J.,
            Uitterlinden,A.G., Uusitupa,M., van der Harst,P., Vollenweider,P.,
            Wallaschofski,H., Wareham,N.J., Watkins,H., Wichmann,H.E.,
            Wilson,J.F., Abecasis,G.R., Assimes,T.L., Barroso,I., Boehnke,M.,
            Borecki,I.B., Deloukas,P., Fox,C.S., Frayling,T., Groop,L.C.,
            Haritunian,T., Heid,I.M., Hunter,D., Kaplan,R.C., Karpe,F.,
            Moffatt,M.F., Mohlke,K.L., O'Connell,J.R., Pawitan,Y., Schadt,E.E.,
            Schlessinger,D., Steinthorsdottir,V., Strachan,D.P.,
            Thorsteinsdottir,U., van Duijn,C.M., Visscher,P.M., Di Blasio,A.M.,
            Hirschhorn,J.N., Lindgren,C.M., Morris,A.P., Meyre,D., Scherag,A.,
            McCarthy,M.I., Speliotes,E.K., North,K.E., Loos,R.J. and
            Ingelsson,E.
  TITLE     Genome-wide meta-analysis identifies 11 new loci for anthropometric
            traits and provides insights into genetic architecture
  JOURNAL   Nat Genet 45 (5), 501-512 (2013)
   PUBMED   23563607
REFERENCE   9  (bases 1 to 3366)
  AUTHORS   Astuti,D., Morris,M.R., Cooper,W.N., Staals,R.H., Wake,N.C.,
            Fews,G.A., Gill,H., Gentle,D., Shuib,S., Ricketts,C.J., Cole,T.,
            van Essen,A.J., van Lingen,R.A., Neri,G., Opitz,J.M., Rump,P.,
            Stolte-Dijkstra,I., Muller,F., Pruijn,G.J., Latif,F. and Maher,E.R.
  TITLE     Germline mutations in DIS3L2 cause the Perlman syndrome of
            overgrowth and Wilms tumor susceptibility
  JOURNAL   Nat Genet 44 (3), 277-284 (2012)
   PUBMED   22306653
  REMARK    GeneRIF: Germline mutations in DIS3L2 cause the Perlman syndrome of
            overgrowth and Wilms tumor susceptibility.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 3366)
  AUTHORS   Turner,J.T., Brzezinski,J. and Dome,J.S.
  TITLE     Wilms Tumor Predisposition
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301471
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA058505.1, BC036113.1,
            BG746662.1 and BM128478.1.
            
            On Dec 27, 2019 this sequence version replaced NM_152383.4.
            
            Summary: The protein encoded by this gene is similar in sequence to
            3'/5' exonucleolytic subunits of the RNA exosome. The exosome is a
            large multimeric ribonucleotide complex responsible for degrading
            various RNA substrates. Several transcript variants, some
            protein-coding and some not, have been found for this gene.
            [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This sequence is assembled by in silico methods with
            additional support of similarity to proteins contained in mouse
            BC036177.1 and Xenopus BC121425.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.16790.1,
                                           SRR11853567.18264.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325385.12/ ENSP00000315569.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-425               DA058505.1         132-556
            426-1705            BC036113.1         455-1734
            1706-2390           BG746662.1         43-727
            2391-2906           BC036113.1         2347-2862
            2907-3366           BM128478.1         2-461               c
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..3366
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /note="DIS3 like 3'-5' exoribonuclease 2"
                     /db_xref="GeneID:129563"
                     /db_xref="HGNC:HGNC:28648"
                     /db_xref="MIM:614184"
     exon            1..53
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            54..198
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..2804
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     family with sequence similarity 6, member A; DIS3-like
                     exonuclease 2; DIS3 mitotic control homolog-like 2"
                     /codon_start=1
                     /product="DIS3-like exonuclease 2 isoform 1"
                     /protein_id="NP_689596.4"
                     /db_xref="CCDS:CCDS42834.1"
                     /db_xref="GeneID:129563"
                     /db_xref="HGNC:HGNC:28648"
                     /db_xref="MIM:614184"
                     /translation="MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRG
                     KKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVAR
                     NRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYND
                     SPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCIS
                     QDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVP
                     RIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETE
                     GILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDD
                     ALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCE
                     ELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKI
                     PAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFVDGALRLDQLKLAFTLDHETGL
                     PQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAFPEQALLRRHPPPQTRMLSDLVEF
                     CDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVLTNMCSRPMQMALYFCSGLLQD
                     PAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAALGYRERLDMAPDTLQKQADHCN
                     DRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCN
                     ALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAESTALKYSAI
                     LKRPGTQGHLGPEKEEEESDGEPEDSSTS"
     exon            199..356
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            357..410
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            411..512
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            513..747
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            748..848
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1096
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1270
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1350
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1463
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1571
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1805
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1885
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2069
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2156
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2304
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2435
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2540
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2642
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..3366
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3128..3133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /note="hexamer: AATAAA"
     polyA_site      3151
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /note="major polyA site"
     regulatory      3343..3348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
                     /note="hexamer: ATTAAA"
     polyA_site      3366
                     /gene="DIS3L2"
                     /gene_synonym="FAM6A; hDIS3L2; PRLMNS"
ORIGIN      
        1 attctcctta gcaactgcgg gactgcggcg gcgccggcct ccggggagaa acgcgaatga
       61 caacagagct gctcaaggcg ggaactctga gctaagcagt ggaggtttct ctggatctgg
      121 agagaagagt gaccttggag ccaataatga gccatcctga ctacagaatg aacctccggc
      181 ccctggggac ccccagaggt gtgtctgctg tggctggtcc acatgacatt ggtgcttcgc
      241 caggtgacaa aaagtcaaag aacaggtcca cacgagggaa gaaaaagagc atatttgaaa
      301 cttacatgtc caaggaggat gtttcagaag gcttgaagag aggaacactc atccagggtg
      361 tattgagaat taatccaaag aagtttcatg aagccttcat tccttccccg gatggtgatc
      421 gagacatttt tattgatggg gttgttgctc gtaatagagc cttaaatggg gatctggtgg
      481 tcgtgaaact gcttcccgag gagcattgga aggtagttaa accagagagc aatgacaaag
      541 aaacagaagc tgcgtatgaa tcagatatcc ccgaggagct ctgtggacac catctcccgc
      601 aacagtccct gaaaagctat aatgacagtc ctgatgtcat tgtagaggct cagtttgatg
      661 gcagcgactc agaagatgga catggcatca cacaaaatgt gctggttgat ggtgttaaga
      721 aactctcagt ttgtgtttct gagaaaggaa gagaggatgg tgatgcaccg gttacaaaag
      781 atgagaccac ctgcatttca caagacacaa gagctttatc ggagaaatcc ctgcaaagat
      841 cagcaaaggt ggtttacatc ttggagaaaa aacattctcg agcagcaacc ggcttcctca
      901 aactcttggc tgataagaac agcgaactgt ttaggaaata cgccctgttt tctccctcag
      961 accaccgagt gcctagaatt tatgtgcctc tcaaggactg tccccaggac tttgtggcac
     1021 ggcctaaaga ttatgccaac acactgttca tctgccgcat tgtggactgg aaggaggact
     1081 gcaattttgc cctggggcag ctggctaaga gtcttgggca ggctggtgaa attgagcctg
     1141 aaacagaagg aatactaaca gagtatggcg tggatttctc tgatttctct tcagaagttc
     1201 tagaatgtct tcctcaaggc ctgccatgga caattccacc agaggagttc agcaagagaa
     1261 gggatttaag aaaagactgt atcttcacca ttgacccatc aaccgcccga gacctcgatg
     1321 atgccctctc ctgcaagcca ctcgctgacg gcaacttcaa agtgggagtt cacattgctg
     1381 acgtgagtta ctttgttccg gagggatctg atctggataa agtggctgcc gagagggcta
     1441 caagcgtcta cttggttcaa aaggtggtcc ccatgcttcc caggctgctg tgtgaggagc
     1501 tgtgcagcct caaccccatg tccgacaagc tgaccttctc tgtgatctgg acactgactc
     1561 cagagggcaa gatccttgat gaatggtttg gccggaccat catccgctcc tgcaccaaac
     1621 ttagctacga gcatgcacag agcatgattg aaagcccaac tgagaaaatc cctgcgaaag
     1681 agctgccccc catttcccca gagcatagca gcgaggaggt acaccaggcc gtcttgaatc
     1741 tccacggaat tgccaagcag ttacgccagc agcgctttgt ggacggcgca cttcgtttgg
     1801 atcagctaaa gcttgctttc actctggacc acgagaccgg attgcctcaa ggatgtcata
     1861 tctatgagta ccgcgagagc aacaagctcg tggaggagtt catgctcttg gccaacatgg
     1921 cagtggccca caagatccac cgcgccttcc ccgagcaggc cctgctgcgc cggcaccccc
     1981 cgccccaaac aaggatgctc agtgacctgg tggaattctg cgaccagatg gggctgcccg
     2041 tggacttcag ctccgcagga gccctcaata aaagcctgac ccaaacattt ggagatgaca
     2101 agtactcact ggcccgcaag gaggtgctca ccaacatgtg ctcccggccc atgcagatgg
     2161 cactgtactt ctgctcgggg ctgctgcagg acccagcgca gttccggcac tacgcgctca
     2221 atgtgcccct gtacacacac ttcacctcgc ccatccgccg ctttgccgac gtcctggtgc
     2281 accgcctcct ggctgccgcg ttaggctata gggagcgact agacatggcg cccgataccc
     2341 tgcagaaaca ggcggaccac tgtaacgacc gccgcatggc gtccaagcgc gtgcaggagc
     2401 tcagtaccag tctcttcttt gctgttctgg tcaaggagag tggccccctg gagtcagaag
     2461 ccatggtgat gggcatcctg aagcaagcct tcgacgtgct ggtgctgcgc tacggcgtgc
     2521 agaagcgcat ctactgcaac gcactggccc tgcggtccca ccacttccag aaggtgggca
     2581 agaagccgga actcacgctg gtctgggagc ctgaggacat ggagcaggag ccagcacagc
     2641 aggtcatcac catcttcagc ctggtggagg tggtcctgca ggcagagtcc acagccctca
     2701 agtacagcgc catcctgaag cggccaggca cccagggcca cctgggccct gagaaggagg
     2761 aggaggagtc tgacggtgag cccgaggact caagcaccag ctgagctcca ccagccgcct
     2821 gccccgcctg ccccgcctgc ctgtcccgcc acactggctt taggacctgt tgacacggag
     2881 gggggttttt aatttggttt ttaacaactc aggggtttgt ttttattttt atttaatttt
     2941 tgcagctcaa cttttaaaca aactgcaggg gagagggtgg ggctggaagg aaggctgagg
     3001 cctggtcagc agtgacccca gcagagcagg ccccagtcct cctgggaggc tggcccccct
     3061 tttttctggg ccctactgcc ctcctctgcc caggaaatgg gggggtttca gcaactcagt
     3121 gtcacagaat aaaatcaagt gtggagtgcc atctggtgtg tagggcgcct ctgggaagcc
     3181 tgggcagcag aatgcccctt gcacccaggg caagggaccc agttcaggct tcacccctcg
     3241 ctgctgagcc gatgtcaaca cctggaactt tcctgtcagt tccaacacga ttcagagctg
     3301 gctgcctggc agatgattga tactggagtc tcattctgcc tgattaaaaa tggaattagt
     3361 atgcaa
//
