LOCUS       NM_004675               1481 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.
ACCESSION   NM_004675
VERSION     NM_004675.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Hutchins,C.M. and Gorfe,A.A.
  TITLE     Intrinsically Disordered Membrane Anchors of Rheb, RhoA, and DiRas3
            Small GTPases: Molecular Dynamics, Membrane Organization, and
            Interactions
  JOURNAL   J Phys Chem B 128 (27), 6518-6528 (2024)
   PUBMED   38942776
  REMARK    GeneRIF: Intrinsically Disordered Membrane Anchors of Rheb, RhoA,
            and DiRas3 Small GTPases: Molecular Dynamics, Membrane
            Organization, and Interactions.
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Bildik,G., Gray,J.P., Mao,W., Yang,H., Ozyurt,R., Orellana,V.R., De
            Wever,O., Carey,M.S., Bast,R.C. and Lu,Z.
  TITLE     DIRAS3 induces autophagy and enhances sensitivity to
            anti-autophagic therapy in KRAS-driven pancreatic and ovarian
            carcinomas
  JOURNAL   Autophagy 20 (3), 675-691 (2024)
   PUBMED   38169324
  REMARK    GeneRIF: DIRAS3 induces autophagy and enhances sensitivity to
            anti-autophagic therapy in KRAS-driven pancreatic and ovarian
            carcinomas.
REFERENCE   3  (bases 1 to 1481)
  AUTHORS   Soboska,K., Kusinski,M., Pawelczyk,K., Migdalska-Sek,M.,
            Brzezianska-Lasota,E. and Czarnecka-Chrebelska,K.H.
  TITLE     Expression of RASSF1A, DIRAS3, and AKAP9 Genes in Thyroid Lesions:
            Implications for Differential Diagnosis and Prognosis of Thyroid
            Carcinomas
  JOURNAL   Int J Mol Sci 25 (1), 562 (2024)
   PUBMED   38203733
  REMARK    GeneRIF: Expression of RASSF1A, DIRAS3, and AKAP9 Genes in Thyroid
            Lesions: Implications for Differential Diagnosis and Prognosis of
            Thyroid Carcinomas.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1481)
  AUTHORS   Yotova,I., Proestling,K., Haslinger,I., Witzmann-Stern,M.,
            Widmar,B., Kuessel,L., Husslein,H., Wenzl,R. and Hudson,Q.J.
  TITLE     DIRAS3 regulates autophagy in an endometriosis epithelial cell line
  JOURNAL   Reprod Biomed Online 47 (4), 103251 (2023)
   PUBMED   37598541
  REMARK    GeneRIF: DIRAS3 regulates autophagy in an endometriosis epithelial
            cell line.
REFERENCE   5  (bases 1 to 1481)
  AUTHORS   Liu,X., Zhang,T., Li,Y., Zhang,Y., Zhang,H., Wang,X. and Li,L.
  TITLE     The Role of Methylation in the CpG Island of the ARHI Promoter
            Region in Cancers
  JOURNAL   Adv Exp Med Biol 1255, 123-132 (2020)
   PUBMED   32949395
  REMARK    GeneRIF: The Role of Methylation in the CpG Island of the ARHI
            Promoter Region in Cancers.
            Review article
REFERENCE   6  (bases 1 to 1481)
  AUTHORS   Luo,R.Z., Fang,X., Marquez,R., Liu,S.Y., Mills,G.B., Liao,W.S.,
            Yu,Y. and Bast,R.C.
  TITLE     ARHI is a Ras-related small G-protein with a novel N-terminal
            extension that inhibits growth of ovarian and breast cancers
  JOURNAL   Oncogene 22 (19), 2897-2909 (2003)
   PUBMED   12771940
  REMARK    GeneRIF: ARHI inhibits cell growth, and loss of its expression in
            cells may contribute to the development of breast and ovarian
            cancers
REFERENCE   7  (bases 1 to 1481)
  AUTHORS   Kontani,K., Tada,M., Ogawa,T., Okai,T., Saito,K., Araki,Y. and
            Katada,T.
  TITLE     Di-Ras, a distinct subgroup of ras family GTPases with unique
            biochemical properties
  JOURNAL   J Biol Chem 277 (43), 41070-41078 (2002)
   PUBMED   12194967
REFERENCE   8  (bases 1 to 1481)
  AUTHORS   Shi,Z., Zhou,X., Xu,L., Zhang,T., Hou,Y., Zhu,W. and Zhang,T.
  TITLE     [NOEY2 gene mRNA expression in breast cancer tissue and its
            relation to clinicopathological parameters]
  JOURNAL   Zhonghua Zhong Liu Za Zhi 24 (5), 475-478 (2002)
   PUBMED   12485503
  REMARK    GeneRIF: A link between NOEY2 loss expression and the spreading
            mechanism of breast cancer may possibly exist.
REFERENCE   9  (bases 1 to 1481)
  AUTHORS   Luo,R.Z., Peng,H., Xu,F., Bao,J., Pang,Y., Pershad,R., Issa,J.P.,
            Liao,W.S., Bast,R.C. Jr. and Yu,Y.
  TITLE     Genomic structure and promoter characterization of an imprinted
            tumor suppressor gene ARHI
  JOURNAL   Biochim Biophys Acta 1519 (3), 216-222 (2001)
   PUBMED   11418188
REFERENCE   10 (bases 1 to 1481)
  AUTHORS   Yu,Y., Xu,F., Peng,H., Fang,X., Zhao,S., Li,Y., Cuevas,B.,
            Kuo,W.L., Gray,J.W., Siciliano,M., Mills,G.B. and Bast,R.C. Jr.
  TITLE     NOEY2 (ARHI), an imprinted putative tumor suppressor gene in
            ovarian and breast carcinomas
  JOURNAL   Proc Natl Acad Sci U S A 96 (1), 214-219 (1999)
   PUBMED   9874798
  REMARK    GeneRIF: DIRAS3 gene is imprinted, with monoallelic expression from
            the paternal allele.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157407.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 23, 2021 this sequence version replaced NM_004675.4.
            
            Summary: This gene encodes a member of the ras superfamily. This
            gene is imprinted gene with monoallelic expression of the paternal
            allele which is associated with growth suppression. The encoded
            protein acts as a tumor suppressor whose function is abrogated in
            many ovarian and breast cancers. This protein may also play a role
            autophagy in certain cancer cells by regulating the autophagosome
            initiation complex. [provided by RefSeq, Nov 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U96750.1, BC005362.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 9874798
            MANE Ensembl match     :: ENST00000646789.1/ ENSP00000495736.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AL157407.11        79086-79165         c
            81-1481             AL157407.11        74500-75900         c
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..1481
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /note="DIRAS family GTPase 3"
                     /db_xref="GeneID:9077"
                     /db_xref="HGNC:HGNC:687"
                     /db_xref="MIM:605193"
     exon            1..80
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /inference="alignment:Splign:2.1.0"
     exon            81..1481
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..835
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /note="ras homolog gene family, member I; GTP-binding
                     protein Di-Ras3; rho-related GTP-binding protein RhoI;
                     distinct subgroup of the Ras family member 3; DIRAS
                     family, GTP-binding RAS-like 3"
                     /codon_start=1
                     /product="GTP-binding protein Di-Ras3 precursor"
                     /protein_id="NP_004666.1"
                     /db_xref="CCDS:CCDS641.1"
                     /db_xref="GeneID:9077"
                     /db_xref="HGNC:HGNC:687"
                     /db_xref="MIM:605193"
                     /translation="MGNASFGSKEQKLLKRLRLLPALLILRAFKPHRKIRDYRVVVVG
                     TAGVGKSTLLHKWASGNFRHEYLPTIENTYCQLLGCSHGVLSLHITDSKSGDGNRALQ
                     RHVIARGHAFVLVYSVTKKETLEELKAFYELICKIKGNNLHKFPIVLVGNKSDDTHRE
                     VALNDGATCAMEWNCAFMEISAKTDVNVQELFHMLLNYKKKPTTGLQEPEKKSQMPNT
                     TEKLLDKCIIM"
     regulatory      1459..1464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /note="hexamer: AATAAA"
     polyA_site      1481
                     /gene="DIRAS3"
                     /gene_synonym="ARHI; NOEY2"
                     /note="major polyA site"
ORIGIN      
        1 gtgtccagtt ggttgccgcg gcagtctctc cgagcagcgc atttgtcttc taggctgctt
       61 ggttcgtgcc tccgagaaag gggtctcctg ctgccagcta agtgtgggag aacttgtgca
      121 cgtatctccc ctccgaatcc caacgatggg taacgccagc tttggctcca aggaacagaa
      181 gctgctgaag cggttgcggc ttctgcccgc cctgcttatc ctccgcgcct tcaagcccca
      241 caggaagatc agagattacc gcgtcgtggt agtcggcacc gctggtgtgg ggaaaagtac
      301 gctgctgcac aagtgggcga gcggcaactt ccgtcatgag tacctgccga ccattgaaaa
      361 tacctactgc cagttgctgg gctgcagcca cggtgtgctt tccctgcaca tcaccgacag
      421 caagagtggc gacggcaacc gcgctctgca gcgccacgtt atagcccggg gccacgcctt
      481 cgtcctggtc tactcagtca ccaagaagga aaccctggaa gagctgaagg ccttctatga
      541 gctgatctgc aagatcaaag gtaacaacct gcataagttc cccatcgtgc tggtgggcaa
      601 taaaagtgat gacacccacc gggaggtggc cctgaatgat ggtgccacct gtgcgatgga
      661 gtggaattgc gccttcatgg agatttcagc caagaccgat gtgaatgtgc aggagctgtt
      721 ccacatgctg ctgaattaca agaaaaagcc caccaccggc ctccaggagc ccgagaagaa
      781 atcccagatg cccaacacca ctgagaagct gcttgacaag tgcataatca tgtgagccct
      841 gggccttaag agccagctct tcctatcctg tagcgtgtag aaaacgtgga ctcatttcac
      901 tatgttacat gtacatggtt gattttgtgc tgttgtttgg actgtaacat ccatgttgtc
      961 aatacgtata ccttgtaagt ggataacttt tctttttccc aggccagaga attcaaattg
     1021 ttaaaacatt ggcatttgaa gaggagaaca aaatgtagca tgatgtattt aaagtaaggc
     1081 ctttagtaat gaatgtaatg agagaaaatg ttttgaaaag aacaaaacat caaaatgaat
     1141 agaaagaaaa attggaaggc gtccttttgg taacccgatt attgtgtatt acctttaaat
     1201 atttcacatc ctgtaagtgc ttaatcatat cttttaattg tgtatttaag aaaagtgttt
     1261 tcacaacaaa agcttttgat aaattgctgc gtgacatata ctaaataaaa aaatgaatat
     1321 gttgatcatt aggggtgtgg gagcagagaa aattgtgaaa gtgactctca ctaaagatgt
     1381 tagtagtttc tcatgtcatt taaaaatgtt tgagtattct gcatagcagt ttgtaaaagt
     1441 gtaacagctt attgacttaa taaagctttt cctgcatgca a
//
