LOCUS       NM_145173               3378 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens DIRAS family GTPase 1 (DIRAS1), mRNA.
ACCESSION   NM_145173
VERSION     NM_145173.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Wang,Y.Y., Ye,L.H., Zhao,A.Q., Gao,W.R., Dai,N., Yin,Y. and
            Zhang,X.
  TITLE     M6A modification regulates tumor suppressor DIRAS1 expression in
            cervical cancer cells
  JOURNAL   Cancer Biol Ther 25 (1), 2306674 (2024)
   PUBMED   38372700
  REMARK    GeneRIF: M6A modification regulates tumor suppressor DIRAS1
            expression in cervical cancer cells.
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Sommerer,Y., Dobricic,V., Schilling,M., Ohlei,O., Sabet,S.S.,
            Wesse,T., Fuss,J., Franzenburg,S., Franke,A., Parkkinen,L.,
            Lill,C.M. and Bertram,L.
  TITLE     Entorhinal cortex epigenome-wide association study highlights four
            novel loci showing differential methylation in Alzheimer's disease
  JOURNAL   Alzheimers Res Ther 15 (1), 92 (2023)
   PUBMED   37149695
  REMARK    GeneRIF: Entorhinal cortex epigenome-wide association study
            highlights four novel loci showing differential methylation in
            Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3378)
  AUTHORS   Liu,H., Shu,W., Liu,T., Li,Q. and Gong,M.
  TITLE     Analysis of the function and mechanism of DIRAS1 in osteosarcoma
  JOURNAL   Tissue Cell 76, 101794 (2022)
   PUBMED   35413492
  REMARK    GeneRIF: Analysis of the function and mechanism of DIRAS1 in
            osteosarcoma.
REFERENCE   4  (bases 1 to 3378)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3378)
  AUTHORS   Wielaender,F., Sarviaho,R., James,F., Hytonen,M.K., Cortez,M.A.,
            Kluger,G., Koskinen,L.L., Arumilli,M., Kornberg,M.,
            Bathen-Noethen,A., Tipold,A., Rentmeister,K., Bhatti,S.F.,
            Hulsmeyer,V., Boettcher,I.C., Tastensen,C., Flegel,T., Dietschi,E.,
            Leeb,T., Matiasek,K., Fischer,A. and Lohi,H.
  TITLE     Generalized myoclonic epilepsy with photosensitivity in juvenile
            dogs caused by a defective DIRAS family GTPase 1
  JOURNAL   Proc Natl Acad Sci U S A 114 (10), 2669-2674 (2017)
   PUBMED   28223533
  REMARK    GeneRIF: This neuronal DIRAS1 gene with a proposed role in
            cholinergic transmission provides not only a candidate for human
            myoclonic epilepsy but also insights into the disease etiology,
            while establishing a spontaneous model for future intervention
            studies and functional characterization.
REFERENCE   6  (bases 1 to 3378)
  AUTHORS   Bergom,C., Hauser,A.D., Rymaszewski,A., Gonyo,P., Prokop,J.W.,
            Jennings,B.C., Lawton,A.J., Frei,A., Lorimer,E.L.,
            Aguilera-Barrantes,I., Mackinnon,A.C., Noon,K., Fierke,C.A. and
            Williams,C.L.
  TITLE     The Tumor-suppressive Small GTPase DiRas1 Binds the Noncanonical
            Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS
            Interactions with Oncogenic Small GTPases
  JOURNAL   J Biol Chem 291 (12), 6534-6545 (2016)
   PUBMED   26814130
  REMARK    GeneRIF: DiRas1 expression inhibits malignant features of cancers
            in part by nonproductively binding to SmgGDS and inhibiting the
            binding of other small GTPases to SmgGDS
            Erratum:[J Biol Chem. 2016 May 13;291(20):10948. doi:
            10.1074/jbc.A115.696831.. PMID: 27197236]
REFERENCE   7  (bases 1 to 3378)
  AUTHORS   Deng,X., Sabino,E.C., Cunha-Neto,E., Ribeiro,A.L., Ianni,B.,
            Mady,C., Busch,M.P. and Seielstad,M.
  CONSRTM   REDSII Chagas Study Group from the NHLBI Retrovirus Epidemiology
            Donor Study-II Component International
  TITLE     Genome wide association study (GWAS) of Chagas cardiomyopathy in
            Trypanosoma cruzi seropositive subjects
  JOURNAL   PLoS One 8 (11), e79629 (2013)
   PUBMED   24324551
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3378)
  AUTHORS   Zhu,Y.H., Fu,L., Chen,L., Qin,Y.R., Liu,H., Xie,F., Zeng,T.,
            Dong,S.S., Li,J., Li,Y., Dai,Y., Xie,D. and Guan,X.Y.
  TITLE     Downregulation of the novel tumor suppressor DIRAS1 predicts poor
            prognosis in esophageal squamous cell carcinoma
  JOURNAL   Cancer Res 73 (7), 2298-2309 (2013)
   PUBMED   23436800
  REMARK    GeneRIF: DIRAS1 downregulation is associated with esophageal
            squamous cell carcinoma metastasis.
REFERENCE   9  (bases 1 to 3378)
  AUTHORS   Kontani,K., Tada,M., Ogawa,T., Okai,T., Saito,K., Araki,Y. and
            Katada,T.
  TITLE     Di-Ras, a distinct subgroup of ras family GTPases with unique
            biochemical properties
  JOURNAL   J Biol Chem 277 (43), 41070-41078 (2002)
   PUBMED   12194967
REFERENCE   10 (bases 1 to 3378)
  AUTHORS   Ellis,C.A., Vos,M.D., Howell,H., Vallecorsa,T., Fults,D.W. and
            Clark,G.J.
  TITLE     Rig is a novel Ras-related protein and potential neural tumor
            suppressor
  JOURNAL   Proc Natl Acad Sci U S A 99 (15), 9876-9881 (2002)
   PUBMED   12107278
  REMARK    GeneRIF: a novel Ras-related protein and potential neural tumor
            suppressor (RIG)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA754806.1, BC030660.1, AK124445.1 and BC017484.1.
            
            On Nov 22, 2018 this sequence version replaced NM_145173.3.
            
            Summary: DIRAS1 belongs to a distinct branch of the functionally
            diverse Ras (see HRAS; MIM 190020) superfamily of monomeric
            GTPases.[supplied by OMIM, Apr 2004].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030660.1, SRR3476690.823811.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323469.5/ ENSP00000325836.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-547               DA754806.1         21-567
            548-754             BC030660.1         547-753
            755-1131            AK124445.1         86-462
            1132-3375           BC017484.1         1-2244
            3376-3378           BC030660.1         3375-3377
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..3378
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /note="DIRAS family GTPase 1"
                     /db_xref="GeneID:148252"
                     /db_xref="HGNC:HGNC:19127"
                     /db_xref="MIM:607862"
     exon            1..69
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /inference="alignment:Splign:2.1.0"
     exon            70..3378
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..735
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /note="small GTP-binding tumor suppressor 1; ras-related
                     inhibitor of cell growth; distinct subgroup of the Ras
                     family member 1; DIRAS family, GTP-binding RAS-like 1"
                     /codon_start=1
                     /product="GTP-binding protein Di-Ras1"
                     /protein_id="NP_660156.1"
                     /db_xref="CCDS:CCDS12092.1"
                     /db_xref="GeneID:148252"
                     /db_xref="HGNC:HGNC:19127"
                     /db_xref="MIM:607862"
                     /translation="MPEQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRDTYIPTIEDTY
                     RQVISCDKSVCTLQITDTTGSHQFPAMQRLSISKGHAFILVFSVTSKQSLEELGPIYK
                     LIVQIKGSVEDIPVMLVGNKCDETQREVDTREAQAVAQEWKCAFMETSAKMNYNVKEL
                     FQELLTLETRRNMSLNIDGKRSGKQKRTDRVKGKCTLM"
     regulatory      3348..3353
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /note="hexamer: AATAAA"
     polyA_site      3378
                     /gene="DIRAS1"
                     /gene_synonym="Di-Ras1; GBTS1; RIG"
                     /note="major polyA site"
ORIGIN      
        1 ggcggcgcca gcggacaccg gagcgggcag gagcagcggc cgcggcgccg cagggaccag
       61 cgggcccagg ctcggtctgg ggttcttgca gctggcacca gccctcccgg ccccggccgc
      121 ccacccgccg cggggaagat gccggaacag agtaacgatt accgcgtggt ggtgttcggg
      181 gcgggcggcg tgggcaagag ctcgctggtg ctgcgcttcg tgaagggcac gttccgcgac
      241 acctacatcc ccaccatcga ggacacctac cggcaggtga tcagctgcga caagagcgtg
      301 tgcacgctgc agatcacaga caccaccggc agccaccagt tcccggccat gcagcgcctg
      361 tccatctcca agggccacgc cttcatcctg gtgttctccg tcaccagcaa gcagtcgctg
      421 gaggagctgg ggcccatcta caagctcatc gtgcagatca agggcagcgt ggaggacatc
      481 cccgtgatgc tcgtgggcaa caagtgcgat gagacgcagc gggaggtgga cacgcgcgag
      541 gcgcaggcgg tggcccagga gtggaagtgc gctttcatgg agacctcggc caagatgaac
      601 tacaacgtca aggagctctt ccaggagctg ctgacgctgg agacgcgccg gaacatgagc
      661 ctcaacatcg acggcaagcg ctccgggaag cagaagagga cagaccgcgt caagggcaaa
      721 tgcaccctca tgtgagcccg gaacgcccgc ctgcccgccg cccaccccca ctgacccccg
      781 ctgcctcccc caacaccgac accctcctcg gcctcctcct cttctctcat ccttcctccg
      841 acacctcggc tggggaaacc gaggccaccg cccccccctc cgctgcccct gcccaccccg
      901 aggcagggct ggggcttttc ttcctcccct gctctctctc caccctcctg ttctgtctgt
      961 accctccaaa accaagagcc ggaggtggcc cccttgtcct gcagatggga aaacaggatg
     1021 gggagctggc aagaggagct gcttgttccc accaggacca gaggaggctg cgtttccccg
     1081 tttccatctc tttccctggg gtgtccccag ccagacctgc gcgtcctgtc cctcacattt
     1141 gatcactgtg accttctggg ggagggggga gttgaaaatg cacatcggcc tcagatattt
     1201 ttttcttttt tctcctattt ggtgttaaca tacacccaag cccacccggc ccgtcgtgac
     1261 ctctgatctg tgcccactcc tccggttcca gacgcacctc tctcctctgt cttcacagtg
     1321 gggtgtgggg cccgtgggat gggcctcagg ccaccaggca ataaccacag ggcctgcagc
     1381 agtgcccctg ccagccccga atcccacccc cgggaccagc cacatccaca gcacaactgc
     1441 cccgctggag aggcaccatg ggcgtggagg ggcttcccgg acaccgccca cccgggaccc
     1501 gcctcttcca ccaagacaga gacgttagca acgcatggcg ggtggggacc tggggtgctc
     1561 aggagggggt acccggggcc ccggccagag atacatcaat tacacccccg tggggggaca
     1621 gccgatggga gccagcacca gcaggatccg agggcgcccc ggacagaggt ctgccccacc
     1681 cacttcctcc ccaccacctg tgccccagag agcagggcct gcccgggaag gtggcgtcct
     1741 ggagtcgagt gtacctgcag ccatgaggtt ctgggtgttt tttgagagag tctgagtgac
     1801 accacactcg tgtgacccca cagggttgtg tccaacatac acggaagtgg ctatggaatg
     1861 gtgtatttgt gcaacctggg gtgcgcggat gggtgacttg tatctaagtg catctgcgtg
     1921 tatacctgtg tgtgtctgtc tgggatgata tgtttttgtg gcagtctgtg tgtgtaatag
     1981 tggtgtaggg tatacagaga ggtgggtagt tgtagatacc tgtgtgtggt tgtcagcaag
     2041 actggatatg tgtgaggtgt ctgtgtgaat ctttgtgcct gtatgagcat gactatattt
     2101 tggggagtgg gtgatatggt ttatctgaga gcatttatct gtaaatatgt ttgtcctgat
     2161 tgagggacac gatctgtgtt ccactctata gcaacatgac tctagcaatg tgactttcgg
     2221 ttccaaatct gtatcagtca gctactgctg tgtaacaaat gaccacaaat gtagcaacca
     2281 gaaacaacac atgcttatta tctcatagat tctgtgggtc aagagcctgg gtgcaggttg
     2341 gctgggtcct ctacttggga tctcaggagg ctgcaatcaa agcattcgcc aggcagaggt
     2401 ctcatctgaa ggcctgatcg gggaaggatt tgcttcttag aagctcatgt ggttgttgca
     2461 gcattcagtt ccttgctgtt gcaagactga aggcctcagt tcctcgctgg ctgttggctg
     2521 gaagctgccc tttgttctgt accatgtggg tctctccaca gcggggctcg gagcatggca
     2581 gctaagttag tgagggaagg tgagatggag gttttggtct tattgggtgt gaggaagcaa
     2641 cgtgtgtgtg tgcgcacgcc cttttgtgca gtgagagaga gagagagatt gcacacatgt
     2701 gtctctgtag tcatgtggcc aggtgggact atgtaggtaa cagattgctc gtgtctgatt
     2761 tggtacaagc atgtttgttt tcctctgtgt tcgtgtgagt gtttactcaa caaatgttta
     2821 ttggacacac tcagagagag ggagtgtgca cacgtgcgtg tgtgttgcta tccagcacgt
     2881 ggaccgggct cccagaagag ctggcattgt gtctgagcag agctgggtcc ccccaaaact
     2941 tgggctggcc cagggcccac cagcagctga tgttgcctcc tctcctgtcc tggcagtagc
     3001 ttctgggttc tgaaggtgcc ggagagagtg aggctgggca ggggtctgcg gccctttctc
     3061 agggacacac cctgatagca caatctcctt ggggccctgc ccacctccag gcctctccca
     3121 cctcaggccc tgcccgaccc tggggagaga gggcatctgc aataggaggg gacccgagcc
     3181 tgtcctggct gctggcccat cctgcctggg catccctggt gctggggact gtgccaggcc
     3241 atgcttgctg tgactccgcc cctgccccct ctccccccgc atgtgggtgc ccccactccc
     3301 ccatcgtggg gtctgtgtag ccttcgctct agacatagtc ttcctgcaat aaaaaagtgg
     3361 atcctgcatt ccccacca
//
