LOCUS       NM_001193369            8479 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens death inducer-obliterator 1 (DIDO1), transcript
            variant 5, mRNA.
ACCESSION   NM_001193369
VERSION     NM_001193369.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Zhang,Y., Jiang,J., Zhang,J., Shen,H., Wang,M., Guo,Z., Zang,X.,
            Shi,H., Gao,J., Cai,H., Fang,X., Qian,H., Xu,W. and Zhang,X.
  TITLE     CircDIDO1 inhibits gastric cancer progression by encoding a novel
            DIDO1-529aa protein and regulating PRDX2 protein stability
  JOURNAL   Mol Cancer 20 (1), 101 (2021)
   PUBMED   34384442
  REMARK    GeneRIF: CircDIDO1 inhibits gastric cancer progression by encoding
            a novel DIDO1-529aa protein and regulating PRDX2 protein stability.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 8479)
  AUTHORS   Forghanifard,M.M., Naeimi Khorasanizadeh,P., Abbaszadegan,M.R.,
            Javdani Mallak,A. and Moghbeli,M.
  TITLE     Role of DIDO1 in Progression of Esophageal Squamous Cell Carcinoma
  JOURNAL   J Gastrointest Cancer 51 (1), 83-87 (2020)
   PUBMED   30761474
  REMARK    GeneRIF: Role of DIDO1 in Progression of Esophageal Squamous Cell
            Carcinoma.
REFERENCE   4  (bases 1 to 8479)
  AUTHORS   Mora Gallardo,C., Sanchez de Diego,A., Gutierrez Hernandez,J.,
            Talavera-Gutierrez,A., Fischer,T., Martinez-A,C. and van
            Wely,K.H.M.
  TITLE     Dido3-dependent SFPQ recruitment maintains efficiency in mammalian
            alternative splicing
  JOURNAL   Nucleic Acids Res 47 (10), 5381-5394 (2019)
   PUBMED   30931476
  REMARK    GeneRIF: Dido3 is an adaptor that controls SFPQ utilization in RNA
            splicing
REFERENCE   5  (bases 1 to 8479)
  AUTHORS   Tencer,A.H., Gatchalian,J., Klein,B.J., Khan,A., Zhang,Y.,
            Strahl,B.D., van Wely,K.H.M. and Kutateladze,T.G.
  TITLE     A Unique pH-Dependent Recognition of Methylated Histone H3K4 by PPS
            and DIDO
  JOURNAL   Structure 25 (10), 1530-1539 (2017)
   PUBMED   28919441
  REMARK    GeneRIF: Results identified a novel pH-dependent mechanism by which
            epigenetic reader, the PHD fingers of DIDO, recognize the histone
            mark H3K4me3. The pH-sensing ability might be necessary for normal
            biological processes and those characterized by altered cellular
            pH.
REFERENCE   6  (bases 1 to 8479)
  AUTHORS   Fu,G.K., Wang,J.T., Yang,J., Au-Young,J. and Stuve,L.L.
  TITLE     Circular rapid amplification of cDNA ends for high-throughput
            extension cloning of partial genes
  JOURNAL   Genomics 84 (1), 205-210 (2004)
   PUBMED   15203218
REFERENCE   7  (bases 1 to 8479)
  AUTHORS   Mochi,M., Cevoli,S., Cortelli,P., Pierangeli,G., Soriani,S.,
            Scapoli,C. and Montagna,P.
  TITLE     A genetic association study of migraine with dopamine receptor 4,
            dopamine transporter and dopamine-beta-hydroxylase genes
  JOURNAL   Neurol Sci 23 (6), 301-305 (2003)
   PUBMED   12624717
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 8479)
  AUTHORS   Gomes,I., Sharma,T.T., Edassery,S., Fulton,N., Mar,B.G. and
            Westbrook,C.A.
  TITLE     Novel transcription factors in human CD34 antigen-positive
            hematopoietic cells
  JOURNAL   Blood 100 (1), 107-119 (2002)
   PUBMED   12070015
  REMARK    GeneRIF: Novel transcription factors identified in human CD34
            antigen-positive hematopoietic stem cells.
REFERENCE   9  (bases 1 to 8479)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   10 (bases 1 to 8479)
  AUTHORS   Garcia-Domingo,D., Leonardo,E., Grandien,A., Martinez,P.,
            Albar,J.P., Izpisua-Belmonte,J.C. and Martinez-A,C.
  TITLE     DIO-1 is a gene involved in onset of apoptosis in vitro, whose
            misexpression disrupts limb development
  JOURNAL   Proc Natl Acad Sci U S A 96 (14), 7992-7997 (1999)
   PUBMED   10393935
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA506324.1, AY481572.1,
            AB002331.2 and CK300739.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001193369.1.
            
            Summary: Apoptosis, a major form of cell death, is an efficient
            mechanism for eliminating unwanted cells and is of central
            importance for development and homeostasis in metazoan animals. In
            mice, the death inducer-obliterator-1 gene is upregulated by
            apoptotic signals and encodes a cytoplasmic protein that
            translocates to the nucleus upon apoptotic signal activation. When
            overexpressed, the mouse protein induced apoptosis in cell lines
            growing in vitro. This gene is similar to the mouse gene and
            therefore is thought to be involved in apoptosis. Alternatively
            spliced transcripts have been found for this gene, encoding
            multiple isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (5) contains a different segment
            for its 5' UTR, compared to variant 4. Variants 4 and 5 encode the
            same protein (isoform c).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY481572.1, SRR14038197.1781030.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395343.6/ ENSP00000378752.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 DA506324.1         47-48
            3-1854              AY481572.1         1-1852
            1855-2320           AB002331.2         1877-2342
            2321-8468           AY481572.1         2319-8466
            8469-8479           CK300739.1         24-34               c
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..8479
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /note="death inducer-obliterator 1"
                     /db_xref="GeneID:11083"
                     /db_xref="HGNC:HGNC:2680"
                     /db_xref="MIM:604140"
     exon            1..67
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            68..264
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            265..1105
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..6989
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /note="isoform c is encoded by transcript variant 5;
                     death-associated transcription factor 1"
                     /codon_start=1
                     /product="death-inducer obliterator 1 isoform c"
                     /protein_id="NP_001180298.1"
                     /db_xref="CCDS:CCDS33506.1"
                     /db_xref="GeneID:11083"
                     /db_xref="HGNC:HGNC:2680"
                     /db_xref="MIM:604140"
                     /translation="MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAE
                     ADPLEPPPPQQQLGLSLRRSGRQPKRTERVEQFLTIARRRGRRSMPVSLEDSGEPTSC
                     PATDAETASEGSVESASETRSGPQSASTAVKERPASSEKVKGGDDHDDTSDSDSDGLT
                     LKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAETVGSEASDTVEGVLPSKQE
                     PENDQGVVSQAGKDDRESKLEGKAAQDIKDEEPGDLGRPKPECEGYDPNALYCICRQP
                     HNNRFMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETHSETAD
                     QQEAKWRPGDADGTDCTSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAP
                     GASKCIGPGCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKP
                     SLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACESSTPSWASD
                     HNYNAVKPEKTAAPSPSLLYKSTKEDRRSEEKAAAMAASKKTAPPGSAVGKQPAPRNL
                     VPKKSSFANVAAATPAIKKPPSGFKGTIPKRPWLSATPSSGASAARQAGPAPAAATAA
                     SKKFPGSAALVGAVRKPVVPSVPMASPAPGRLGAMSAAPSQPNSQIRQNIRRSLKEIL
                     WKRVNDSDDLIMTENEVGKIALHIEKEMFNLFQVTDNRYKSKYRSIMFNLKDPKNQGL
                     FHRVLREEISLAKLVRLKPEELVSKELSTWKERPARSVMESRTKLHNESKKTAPRQEA
                     IPDLEDSPPVSDSEEQQESARAVPEKSTAPLLDVFSSMLKDTTSQHRAHLFDLNCKIC
                     TGQVPSAEDEPAPKKQKLSASVKKEDLKSKHDSSAPDPAPDSADEVMPEAVPEVASEP
                     GLESASHPNVDRTYFPGPPGDGHPEPSPLEDLSPCPASCGSGVVTTVTVSGRDPRTAP
                     SSSCTAVASAASRPDSTHMVEARQDVPKPVLTSVMVPKSILAKPSSSPDPRYLSVPPS
                     PNISTSESRSPPEGDTTLFLSRLSTIWKGFINMQSVAKFVTKAYPVSGCFDYLSEDLP
                     DTIHIGGRIAPKTVWDYVGKLKSSVSKELCLIRFHPATEEEEVAYISLYSYFSSRGRF
                     GVVANNNRHVKDLYLIPLSAQDPVPSKLLPFEGPGLESPRPNIILGLVICQKIKRPAN
                     SGELDKMDEKRTRLQPEEADVPAYPKVATVPQSEKKPSKYPLCSADAAVSTTPPGSPP
                     PPPPLPEPPVLKVLSSLKPAAPSPATAATTAAAASTAASSTASSASKTASPLEHILQT
                     LFGKKKSFDPSAREPPGSTAGLPQEPKTTAEDGVPAPPLLDPIVQQFGQFSKDKALEE
                     EEDDRPYDPEEEYDPERAFDTQLVERGRRHEVERAPEAAAAEREEVAYDPEDETILEE
                     AKVTVDDLPNRMCADVRRNSVERPAEPVAGAATPSLVEQQKMLEELNKQIEEQKRQLE
                     EQEEALRQQRAAVGVSMAHFSVSDALMSPPPKSSLPKAELFQQEQQSADKPASLPPAS
                     QASNHRDPRQARRLATETGEGEGEPLSRLSARGAQGALPERDASRGGLVGQAPMPVPE
                     EKEPASSPWASGEKPPAGSEQDGWKAEPGEGTRPATVGDSSARPARRVLLPTPPCGAL
                     QPGFPLQHDGERDPFTCPGFASQDKALGSAQYEDPRNLHSAGRSSSPAGETEGDREPQ
                     ARPGEGTAPLPPPGQKVGGSQPPFQGQREPGPHALGMSGLHGPNFPGPRGPAPPFPEE
                     NIASNDGPRGPPPARFGAQKGPIPSLFSGQHGPPPYGDSRGPSPSYLGGPRGVAPSQF
                     EERKDPHGEKREFQDAPYNEVTGAPAQFEGTEQAPFLGSRGGAPFQFGGQRRPLLSQL
                     KGPRGGPPPSQFGGQRGPPPGHFVGPRGPHPSQFETARGPHPNQFEGPRGQAPNFMPG
                     PRGIQPQQFEDQRVHSPPRFTNQRAPAPLQFGGLRGSAPFSEKNEQTPSRFHFQGQAP
                     QVMKPGPRPLLELPSHPPQHRKDRWEEAGPPSALSSSAPGQGPEADGQWASADFREGK
                     GHEYRNQTFEGRQRERFDVGPKEKPLEEPDAQGRASEDRRRERERGRNWSRERDWDRP
                     REWDRHRDKDSSRDWDRNRERSANRDREREADRGKEWDRSRERSRNRERERDRRRDRD
                     RSRSRERDRDKARDRERGRDRKDRSKSKESARDPKPEASRASDAGTASQA"
     exon            1106..1427
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1640
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1854
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2320
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2480
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2597
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2702
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2838
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..3367
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3521
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            3522..3611
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            3612..3807
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     exon            3808..8479
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8462..8467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /note="hexamer: AATAAA"
     polyA_site      8479
                     /gene="DIDO1"
                     /gene_synonym="BYE1; C20orf158; DATF-1; DATF1; DIDO2;
                     DIDO3; DIO-1; DIO1; dJ885L7.8"
                     /note="major polyA site"
ORIGIN      
        1 atctcggtgg ccgtccgccc actccgcggc gttcggggaa atggctgcga gaccctagag
       61 gcctgcggag cttactccac gggaacagcc tctagataat ctgagttgtt gaaaatacga
      121 agcctgttac tcgtgaacag tggctgacaa cagtgttgtt gtgagcctgg ctgtctgctt
      181 ggacccagag gtttcgtctg ccagggtttt tggttgtatt taggatttca gggaaaagtg
      241 tccaagcttt cagtgttgga gcaggtatgg acgacaaagg cgacccgagc aatgaggagg
      301 cacctaaggc catcaaaccc accagcaaag agttcaggaa aacatggggt tttcgaagga
      361 ccactatcgc caagcgagag ggcgcagggg acgcggaggc tgacccactg gagccgccac
      421 ccccacagca gcagctgggc ctgtccctgc ggcgcagtgg gaggcagccc aagcgcactg
      481 agcgcgtgga gcagttcctg accattgcgc ggcgccgcgg caggaggagc atgcctgtct
      541 ccctggagga ttctggtgag cccacgtcct gccccgccac agacgccgag acagcctccg
      601 agggcagcgt ggaaagcgct tctgagacca gaagcggccc ccagtctgct tccacagctg
      661 tgaaggaacg accagcctct tctgaaaagg tgaaaggagg ggatgaccac gatgacacct
      721 ccgatagtga cagcgatggc ctgaccttga aagagcttca gaatcgcctt cgcaggaagc
      781 gggaacagga gcccactgag aggcccctga aagggatcca gagtcgcctg cggaagaagc
      841 gccgggagga gggtcccgcc gagactgtgg gctccgaggc cagtgacact gtggagggcg
      901 tcctgcccag taagcaggag cccgagaacg atcagggggt tgtgtcccag gctgggaaag
      961 atgacagaga gagtaagttg gagggaaagg cggctcagga catcaaagat gaggagcctg
     1021 gagacttggg ccgaccgaag cctgaatgtg agggttacga ccccaacgcc ctgtattgca
     1081 tttgccgcca gcctcacaac aacaggttta tgatttgctg tgaccgctgt gaagaatggt
     1141 ttcatggcga ttgtgtgggc atttctgagg ctcgagggag gcttttggaa aggaatgggg
     1201 aagactatat ctgcccaaac tgcaccattc tgcaagtgca ggatgagact cattcagaaa
     1261 cggcagatca gcaggaagct aaatggagac ctggagatgc tgatggcacc gattgtacaa
     1321 gtataggaac aatagagcag aagtctagcg aagaccaagg gataaagggt agaattgaga
     1381 aagctgcaaa tccaagtggc aagaagaaac tcaagatctt ccagcctgtg atagaggcgc
     1441 ctggtgcctc aaaatgtatt ggccccgggt gctgtcacgt ggcgcagccc gactcggtgt
     1501 actgcagtaa tgactgtatc ctcaaacacg ccgcagcgac aatgaagttt ctaagctcag
     1561 gtaaagaaca gaagccaaag cctaaagaaa agatgaagat gaagccagag aagcccagtc
     1621 ttccgaaatg cggtgctcag gcaggtatta aaatctcttc tgtgcacaag agaccagctc
     1681 cagaaaaaaa agagaccaca gtgaagaagg cagtggtggt ccctgcgcgg agtgaagcac
     1741 tcgggaagga agcagcttgt gagagcagca cgccgtcgtg ggcgagcgat cacaattaca
     1801 atgcagtaaa gccagaaaag actgctgctc cctcgccgtc actgttgtat aaatccacga
     1861 aggaagacag gaggtccgag gagaaagcgg cagccatggc agcctcaaag aaaacagccc
     1921 ctccaggctc cgcggtgggc aagcagcctg cacctagaaa cctcgtgcca aagaagtctt
     1981 cttttgctaa tgtggcagca gccacaccag ccattaaaaa gccaccctca ggtttcaagg
     2041 gcaccatccc caagaggcca tggctctccg ctaccccatc gagtggtgct tcagctgcca
     2101 ggcaggccgg accggcacct gcagcggcaa cggctgcctc caagaagttc cctggctccg
     2161 ctgctttggt gggagccgta aggaagccag tggtaccttc tgttccaatg gcctcgccag
     2221 ccccaggacg ccttggggct atgagtgctg caccatcgca gccaaattca caaattcggc
     2281 aaaatatcag acgctcctta aaagagattt tgtggaaaag agtcaatgac agcgatgact
     2341 taatcatgac agaaaacgaa gtaggaaaaa ttgccctcca tattgagaag gagatgttta
     2401 acttgtttca agttacagat aatcgctaca agagtaaata tcgcagcatc atgttcaacc
     2461 tgaaggaccc taaaaatcag ggactcttcc atcgtgttct gcgtgaggaa atctctttgg
     2521 cgaaacttgt gagactgaag ccagaagaac ttgtatctaa agagctttcc acgtggaaag
     2581 agaggccagc gagatctgtg atggagtcca gaactaaact gcacaatgaa agcaagaaga
     2641 cggcccccag gcaggaggcc atccccgatc tggaggactc tccgccagtg tcggattcag
     2701 aggaacagca agagtcagca cgtgctgtcc ctgagaagag cacagcgccg cttctcgacg
     2761 tcttcagcag catgttgaaa gacaccacca gtcagcaccg cgcacacctc ttcgatctca
     2821 actgtaaaat ttgcacaggc caggttccct ccgcagaaga tgagccagct ccgaaaaaac
     2881 aaaaattgtc agcttctgtt aagaaagaag acttaaaatc aaagcatgac agctctgcac
     2941 ctgacccagc tccggattca gctgatgagg tgatgccgga ggctgtgcct gaagttgcct
     3001 ctgagccagg cctagaaagt gcttctcatc caaatgtgga cagaacgtat ttccctgggc
     3061 ctccaggaga tggccatccc gagccctccc cgctggaaga cctgtccccc tgcccagcct
     3121 cctgtgggag cggggtggtc accaccgtca cagtgtccgg ccgggacccc aggaccgctc
     3181 caagcagttc atgcacagcc gtggcctccg cagcatcccg cccagacagc acccacatgg
     3241 tggaagccag acaggatgtg ccgaagcctg tcttgacttc tgtgatggtg cccaagtcca
     3301 tactagctaa gccatcctca tctcctgacc caagatacct gtcagttcct ccgtcaccaa
     3361 atatcagcac ttcagaatca cgttcccctc cagagggaga cacgaccctc tttttgtctc
     3421 gactcagcac catttggaaa ggatttatta acatgcagag tgtggcaaaa tttgtcacta
     3481 aggcgtatcc tgtctctggg tgttttgatt acctcagtga ggatttgcct gacacaattc
     3541 acattggtgg gaggatcgca ccgaagacag tttgggatta tgttggcaaa ctcaagtctt
     3601 ctgtgtctaa ggagctctgt ctgatccgct tccaccccgc cacagaggaa gaggaggtcg
     3661 cctatatctc tctctactcc tatttcagca gccgtggccg ctttggtgtt gtagctaata
     3721 acaacaggca cgtcaaggac ctctacctga tcccgctgag cgcccaggac cctgttccat
     3781 ccaaactctt gccctttgag ggaccaggtc ttgagtcacc acgtccgaat ataattcttg
     3841 ggttagtaat ctgccaaaaa atcaaacgtc ccgcaaacag tggagagtta gacaagatgg
     3901 acgaaaagcg gacccgactt caaccggaag aagcggacgt tccggcctat ccaaaagtag
     3961 ccacagtccc gcagtcggaa aagaagccct ccaagtatcc actctgctct gcagacgcgg
     4021 ctgtcagcac cacacctcct gggtcgccgc cgcctccgcc ccctcttcca gaaccaccgg
     4081 tgctaaaagt gctgtcatcc ctcaaacctg cagcccccag cccagccaca gcagccacaa
     4141 cagcagcggc agcctccacg gcagcttcct ccaccgcttc gtctgcttcc aaaacagcat
     4201 caccgctgga gcacatcctg cagactctct ttggaaagaa gaaatcattc gacccgtccg
     4261 ccagagagcc tcccgggtcc accgcaggtc tcccccagga gcccaaaacc acagcagagg
     4321 acggggtgcc ggcacctccg ttgttagatc cgatcgtcca gcagttcggt cagttctcaa
     4381 aagataaggc tctagaggaa gaggaggacg acaggccata cgaccctgag gaggagtacg
     4441 acccggagag ggccttcgac actcagcttg tggagcgagg gcggcgccac gaggtggaaa
     4501 gggctcctga agcagctgca gccgagcggg aagaggtggc ctatgacccc gaggatgaga
     4561 ccatcttaga agaagcgaaa gtgactgttg atgacctgcc caacaggatg tgtgccgacg
     4621 tgagaaggaa ctccgtggag aggcctgccg agccggtggc cggggctgcg acgccctccc
     4681 tggtggagca acagaagatg ctagaagagc tgaacaaaca gatcgaggag cagaagagac
     4741 agctggagga gcaagaagaa gctctcaggc agcagagggc cgccgtcggg gtctccatgg
     4801 cccacttctc ggtgtcggac gccttgatgt ctccaccacc aaagtcgtcc ttgcccaagg
     4861 cagagctgtt ccagcaagag cagcagtctg cagacaagcc cgcctcactg ccccccgcca
     4921 gccaggcgtc aaaccacagg gacccccggc aggcgaggcg cctggccact gagaccggtg
     4981 agggggaggg ggagcctctc tccaggctct cggcacgtgg tgcccagggt gccctgcccg
     5041 agagagatgc ttccaggggt ggcctcgtgg gccaggcgcc catgccagtc ccggaggaaa
     5101 aagagccagc ctcttccccc tgggcttcgg gcgaaaagcc cccagcgggg tccgagcagg
     5161 acggctggaa ggcagagcct ggggagggca cccgccccgc cacggttgga gacagctcgg
     5221 ccaggcctgc ccggagggtg ctgctgccca caccgccttg cggcgccctg cagcccggct
     5281 tcccgctgca gcacgacggt gagagggacc ctttcacctg cccggggttc gcgtcgcagg
     5341 acaaggctct cggctcagcc cagtatgagg acccaagaaa tcttcattct gctggaagga
     5401 gcagcagccc tgcaggtgaa acagaggggg acagagagcc acaggccaga cccggcgagg
     5461 gcaccgcccc gctcccccca ccaggacaga aagtgggggg ctctcagccc ccgtttcagg
     5521 gtcagcggga acctggacct catgctttgg ggatgtcagg gcttcacggc cccaatttcc
     5581 cgggaccaag ggggccagcc cctccgtttc cagaagagaa tatcgcttct aacgatgggc
     5641 cacgagggcc tccgccagcc agattcggag cccagaaggg gcccatccct tccttattct
     5701 cggggcaaca tgggccacct ccttatgggg acagcagagg cccctcaccc tcttaccttg
     5761 gtggaccacg aggagtggca ccatcccaat ttgaagaacg caaggatccc catggggaga
     5821 agagggagtt ccaggacgcc ccgtataacg aggtgacggg cgcccccgcc cagtttgaag
     5881 ggacagagca agccccattt ctgggaagca gaggcggcgc gcctttccag ttcggaggcc
     5941 agagaaggcc actgctgtct cagctgaaag gcccccgagg cggcccccct ccctctcagt
     6001 ttggaggtca gagaggacca ccccctggtc atttcgtggg cccaagaggg ccccatccta
     6061 gtcagtttga aactgcccgg ggccctcatc ccaaccagtt tgaaggaccc agaggccaag
     6121 cgcctaactt tatgccaggt cccaggggca ttcagcctca gcagttcgaa gaccagaggg
     6181 tccattcacc accaagattc acaaaccaaa gggcgcctgc acccctgcag tttggtggac
     6241 tacgggggtc cgcacccttt tctgaaaaaa atgagcagac cccttcgcga tttcacttcc
     6301 agggccaggc cccgcaggtg atgaagccgg gccccaggcc cctgctggag cttcccagcc
     6361 accccccgca gcaccggaag gaccgctggg aggaggccgg gccgccctcc gcgctctcct
     6421 ccagtgcgcc cggacagggc cccgaggccg acggacagtg ggcatcggcc gacttccgag
     6481 aggggaaagg ccacgaatac agaaaccaga ctttcgaagg gaggcagaga gagcggtttg
     6541 acgtggggcc caaagagaag ccgctggagg agcccgacgc ccagggccgg gcgtccgagg
     6601 acaggaggag agagcgcgag cgcggccgaa actggagccg agagcgggac tgggaccggc
     6661 cccgggagtg ggaccgacac cgggacaagg actccagccg ggactgggac agaaaccggg
     6721 agaggagcgc caaccgcgac cgagagcgcg aggccgaccg gggcaaggag tgggaccgca
     6781 gccgggagcg gagcaggaac cgagagcgcg agcgagaccg gaggcgcgac cgggaccggt
     6841 cccggagcag agagcgggac cgagacaagg ccagggacag ggagcggggc cgcgaccgca
     6901 aggaccggag caagagcaaa gagagcgctc gggacccgaa gcccgaggcc tcgagggcct
     6961 ccgacgctgg caccgcctcg caggcctaga cgccccggcc gggcagagac cctttttaaa
     7021 gccacgttcg taagatgcgt tgaacaaata gcttttgaaa tagccacgat cttagattca
     7081 ggatagaata ttctggactt aaacattact gtaattttgt gtaaatggtt tcttacaaga
     7141 tttcacatct ttacaattct gatgcttttt taaaaaaact aaactttaat atttccattt
     7201 aaaattaaaa gaaatgggaa attgcctacg gagcatattg cttttcagat catagttatc
     7261 ttttccaata actttattgt aaattttaag ggagtttcgt tggaccctag agccatacct
     7321 tcactgacat ctcatccgtt tgtgtttcca aaagctgctt ttacaggtct tagccgccgc
     7381 cccggaggcc ggtgtgggcg tgacctgcag gcgtccaccc tgcggctacg ggagaagtct
     7441 tagtagcttg tatctggagt cttgagaaac acaaacctta caaatgcatc tttcttttgt
     7501 cataaaattg gaattacaat tttcaactgg gaaacagaaa cacatttctt agcgtgctca
     7561 gattttatat atcaccttat caattttttc caaaacatga tttaaagcaa catctgtcct
     7621 agtataattg ttgcatttta aataacaatt tattgaaaaa tgttaagttt taccagccta
     7681 aaaaaagtaa caaaccaatt aagtgtactt tgggggcagg gaggagtcct tgatttgaat
     7741 aatcaggagt tttgttggtc cagtttcgcc ttctgagcag ttcttctgta tcccgggaaa
     7801 agtacgctcg gccccaaact ggtatttgtc agacgataca tgtaaaagtt tgtccttgaa
     7861 cgtggaacaa gacttgtgta aagtctgaga cgggaactag tgatcactag ctcaagaact
     7921 gcaaatgaga caggaagctg ttcactgtga aacagtctct tgcttgtcgt gtatttttcg
     7981 agtacttctg aagtgtgaag taaaaaaatc tggctaattt atttgtacaa atacactttg
     8041 ataatttttt atattttcat aaacttttac agatgtaaaa ttaaagaact tcacttgttc
     8101 tttggagtct gttattccat tgtacgtcac tacagatttt tcactttata gcacagattt
     8161 tttaagttaa gaaaacattg agaatgtatc agtttgagat gtttggtgtt gaaagccaag
     8221 tgtccatagg ttaaattaca ttaaagtagg tgtcacgttc caacatctag tacgatgaga
     8281 aggttttgtt tgtttaacat ataaagcaaa tttttctaat ttaaatacac aaatgtaaaa
     8341 atcaagtgtg tttctttagt ttacttgata gagattcctg tacattgtat tttatattgc
     8401 ggaggcttat atcactgtac atgagaacat ggaacgacct aggttttgtt actcgcagta
     8461 gaataaacag ctacaggaa
//
