LOCUS       NM_177438              10384 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant
            1, mRNA.
ACCESSION   NM_177438
VERSION     NM_177438.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Rojo-Pardillo,M., Godefroid,L., Dom,G., Lefort,A., Libert,F.,
            Robaye,B. and Maenhaut,C.
  TITLE     Understanding the Dosage-Dependent Role of Dicer1 in Thyroid
            Tumorigenesis
  JOURNAL   Int J Mol Sci 25 (19), 10701 (2024)
   PUBMED   39409030
  REMARK    GeneRIF: Understanding the Dosage-Dependent Role of Dicer1 in
            Thyroid Tumorigenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Mastnikova,K., Bulanova Pekova,B., Kuklikova,V., Vaclavikova,E.,
            Carkova,J., Katra,R., Fialova,L., Vlcek,P., Kodetova,D.,
            Chovanec,M., Drozenova,J., Matej,R., Pacesova,P., Novak,Z.,
            Procykova,K., Vcelak,J. and Bendlova,B.
  TITLE     DICER1 Variants in Pediatric and Young Adult Thyroid Nodules
  JOURNAL   Thyroid 34 (10), 1225-1233 (2024)
   PUBMED   39283830
  REMARK    GeneRIF: DICER1 Variants in Pediatric and Young Adult Thyroid
            Nodules.
REFERENCE   3  (bases 1 to 10384)
  AUTHORS   Dashti,N.K., Swanson,A.A., Bentz,J., Xing,D., Chrisinger,J.S.A.,
            Balzer,B., Guo,R., Schoolmeester,J.K. and Maluf,H.
  TITLE     DICER1-sarcomas of GYN tract: Expanding on an emerging entity
  JOURNAL   Hum Pathol 152, 105636 (2024)
   PUBMED   39127354
  REMARK    GeneRIF: DICER1-sarcomas of GYN tract: Expanding on an emerging
            entity.
REFERENCE   4  (bases 1 to 10384)
  AUTHORS   Rania,L., Djalila,C., Brahim,D., Rayene,A., Meroua,H., Souhem,T.,
            Zoubir,A., Hichem,C., Asma,B., Dalila,S. and Karima,S.
  TITLE     Potential risk factors for hypospadias and negative correlation
            with DICER1 (rs3742330) A>G variant in Algerian population: A
            case-control study
  JOURNAL   Birth Defects Res 116 (5), e2365 (2024)
   PUBMED   38801237
  REMARK    GeneRIF: Potential risk factors for hypospadias and negative
            correlation with DICER1 (rs3742330) A>G variant in Algerian
            population: A case-control study.
REFERENCE   5  (bases 1 to 10384)
  AUTHORS   Irvin-Wilson,C.V. and Chaudhuri,G.
  TITLE     Alternative initiation and splicing in dicer gene expression in
            human breast cells
  JOURNAL   Breast Cancer Res 7 (4), R563-R569 (2005)
   PUBMED   15987463
REFERENCE   6  (bases 1 to 10384)
  AUTHORS   Bignell,G.R., Canzian,F., Shayeghi,M., Stark,M., Shugart,Y.Y.,
            Biggs,P., Mangion,J., Hamoudi,R., Rosenblatt,J., Buu,P., Sun,S.,
            Stoffer,S.S., Goldgar,D.E., Romeo,G., Houlston,R.S., Narod,S.A.,
            Stratton,M.R. and Foulkes,W.D.
  TITLE     Familial nontoxic multinodular thyroid goiter locus maps to
            chromosome 14q but does not account for familial nonmedullary
            thyroid cancer
  JOURNAL   Am J Hum Genet 61 (5), 1123-1130 (1997)
   PUBMED   9345104
REFERENCE   7  (bases 1 to 10384)
  AUTHORS   Schultz,K.A.P., Stewart,D.R., Kamihara,J., Bauer,A.J.,
            Merideth,M.A., Stratton,P., Huryn,L.A., Harris,A.K., Doros,L.,
            Field,A., Carr,A.G., Dehner,L.P., Messinger,Y. and Hill,D.A.
  TITLE     DICER1 Tumor Predisposition
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24761742
REFERENCE   8  (bases 1 to 10384)
  AUTHORS   Turner,J.T., Brzezinski,J. and Dome,J.S.
  TITLE     Wilms Tumor Predisposition
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301471
REFERENCE   9  (bases 1 to 10384)
  AUTHORS   Couch,R.M., Hughes,I.A., DeSa,D.J., Schiffrin,A., Guyda,H. and
            Winter,J.S.
  TITLE     An autosomal dominant form of adolescent multinodular goiter
  JOURNAL   Am J Hum Genet 39 (6), 811-816 (1986)
   PUBMED   3799599
REFERENCE   10 (bases 1 to 10384)
  AUTHORS   Murray,I.P., Thomson,J.A., McGirr,E.M., Macdonald,E.M.,
            Kennedy,J.S. and McLennan,I.
  TITLE     Unusual familial goiter associated with intrathyroidal
            calcification
  JOURNAL   J Clin Endocrinol Metab 26 (10), 1039-1049 (1966)
   PUBMED   4162574
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356017.3, AB023145.2 and
            AL390254.6.
            
            On Feb 25, 2020 this sequence version replaced NM_177438.2.
            
            Summary: This gene encodes a protein possessing an RNA helicase
            motif containing a DEXH box in its amino terminus and an RNA motif
            in the carboxy terminus. The encoded protein functions as a
            ribonuclease and is required by the RNA interference and small
            temporal RNA (stRNA) pathways to produce the active small RNA
            component that represses gene expression. This protein also acts as
            a strong antiviral agent with activity against RNA viruses,
            including the Zika and SARS-CoV-2 viruses. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jul
            2021].
            
            Transcript Variant: This variant (1) differs in the 5' UTR,
            compared to variant 2. Variants 1, 2, 4 and 5 encode the same
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1120890.1,
                                           SRR14038192.1795776.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000343455.8/ ENSP00000343745.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AL356017.3         80178-80284         c
            108-6144            AB023145.2         1-6037
            6145-10384          AL390254.6         9522-13761
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.13"
     gene            1..10384
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /note="dicer 1, ribonuclease III"
                     /db_xref="GeneID:23405"
                     /db_xref="HGNC:HGNC:17098"
                     /db_xref="MIM:606241"
     exon            1..300
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            301..489
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             346..6114
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /EC_number="3.1.26.3"
                     /note="isoform 1 is encoded by transcript variant 1; dicer
                     1, double-stranded RNA-specific endoribonuclease; helicase
                     with RNAse motif; endoribonuclease Dicer; helicase MOI;
                     Dicer1, Dcr-1 homolog; dicer 1, ribonuclease type III"
                     /codon_start=1
                     /product="endoribonuclease Dicer isoform 1"
                     /protein_id="NP_803187.1"
                     /db_xref="CCDS:CCDS9931.1"
                     /db_xref="GeneID:23405"
                     /db_xref="HGNC:HGNC:17098"
                     /db_xref="MIM:606241"
                     /translation="MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTP
                     RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNS
                     ANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLK
                     NGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEE
                     LEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEE
                     ALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIK
                     HEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYER
                     QQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVER
                     RYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKF
                     RAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADT
                     DKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPR
                     VTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGP
                     PMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRP
                     GSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDT
                     TRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSH
                     ILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKY
                     TKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYY
                     KTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNK
                     QILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPAD
                     FRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENH
                     DQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNY
                     YKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMP
                     GTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGD
                     SFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPV
                     NWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEA
                     DYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPF
                     SSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQ
                     CIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENF
                     NSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFEN
                     FEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQ
                     HSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQG
                     MDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLI
                     EKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIA
                     KSAAARRALRSLKANQPQVPNS"
     exon            490..652
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            653..783
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            784..918
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1079
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1248
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1721
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1854
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2097
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2252
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2385
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2461
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2601
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2781
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2995
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3149
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3332
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3438
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3614
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            3615..4395
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            4396..4551
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            4552..5440
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            5441..5709
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            5710..5872
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            5873..5948
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     exon            5949..10384
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10364..10369
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /note="hexamer: AATAAA"
     polyA_site      10384
                     /gene="DICER1"
                     /gene_synonym="aviD; DCR1; Dicer; Dicer1e; GLOW; HERNA;
                     K12H4.8-LIKE; MNG1; RMSE2"
                     /note="major polyA site"
ORIGIN      
        1 agtgggattc tccaagcggc tgtgcgccgt tgccgcgggc cgtgcgctgg ctgccggcca
       61 gccgggcgct gcgcagtctc cgcaggcgcc ggcgggaggg gtcgaggcgg aggcgcggcg
      121 caggctgctg caggcccagg tgaatggagt aacctgacag cggggacgag gcgacggcga
      181 gcgcgaggaa atggcggcgg gggcggcggc gccgggcggc tccgggaggc ctgggctgtg
      241 acgcgcgcgc cggagcgggg tccgatggtt ctcgaaggcc cgcggcgccc cgtgctgcag
      301 taagctgtgc tagaacaaaa atgcaatgaa agaaacactg gatgaatgaa aagccctgct
      361 ttgcaacccc tcagcatggc aggcctgcag ctcatgaccc ctgcttcctc accaatgggt
      421 cctttctttg gactgccatg gcaacaagaa gcaattcatg ataacattta tacgccaaga
      481 aaatatcagg ttgaactgct tgaagcagct ctggatcata ataccatcgt ctgtttaaac
      541 actggctcag ggaagacatt tattgcagta ctactcacta aagagctgtc ctatcagatc
      601 aggggagact tcagcagaaa tggaaaaagg acggtgttct tggtcaactc tgcaaaccag
      661 gttgctcaac aagtgtcagc tgtcagaact cattcagatc tcaaggttgg ggaatactca
      721 aacctagaag taaatgcatc ttggacaaaa gagagatgga accaagagtt tactaagcac
      781 caggttctca ttatgacttg ctatgtcgcc ttgaatgttt tgaaaaatgg ttacttatca
      841 ctgtcagaca ttaacctttt ggtgtttgat gagtgtcatc ttgcaatcct agaccacccc
      901 tatcgagaaa ttatgaagct ctgtgaaaat tgtccatcat gtcctcgcat tttgggacta
      961 actgcttcca ttttaaatgg gaaatgtgat ccagaggaat tggaagaaaa gattcagaaa
     1021 ctagagaaaa ttcttaagag taatgctgaa actgcaactg acctggtggt cttagacagg
     1081 tatacttctc agccatgtga gattgtggtg gattgtggac catttactga cagaagtggg
     1141 ctttatgaaa gactgctgat ggaattagaa gaagcactta attttatcaa tgattgtaat
     1201 atatctgtac attcaaaaga aagagattct actttaattt cgaaacagat actatcagac
     1261 tgtcgtgccg tattggtagt tctgggaccc tggtgtgcag ataaagtagc tggaatgatg
     1321 gtaagagaac tacagaaata catcaaacat gagcaagagg agctgcacag gaaattttta
     1381 ttgtttacag acactttcct aaggaaaata catgcactat gtgaagagca cttctcacct
     1441 gcctcacttg acctgaaatt tgtaactcct aaagtaatca aactgctcga aatcttacgc
     1501 aaatataaac catatgagcg acagcagttt gaaagcgttg agtggtataa taatagaaat
     1561 caggataatt atgtgtcatg gagtgattct gaggatgatg atgaggatga agaaattgaa
     1621 gaaaaagaga agccagagac aaattttcct tctcctttta ccaacatttt gtgcggaatt
     1681 atttttgtgg aaagaagata cacagcagtt gtcttaaaca gattgataaa ggaagctggc
     1741 aaacaagatc cagagctggc ttatatcagt agcaatttca taactggaca tggcattggg
     1801 aagaatcagc ctcgcaacaa acagatggaa gcagaattca gaaaacagga agaggtactt
     1861 aggaaatttc gagcacatga gaccaacctg cttattgcaa caagtattgt agaagagggt
     1921 gttgatatac caaaatgcaa cttggtggtt cgttttgatt tgcccacaga atatcgatcc
     1981 tatgttcaat ctaaaggaag agcaagggca cccatctcta attatataat gttagcggat
     2041 acagacaaaa taaaaagttt tgaagaagac cttaaaacct acaaagctat tgaaaagatc
     2101 ttgagaaaca agtgttccaa gtcggttgat actggtgaga ctgacattga tcctgtcatg
     2161 gatgatgatg acgttttccc accatatgtg ttgaggcctg acgatggtgg tccacgagtc
     2221 acaatcaaca cggccattgg acacatcaat agatactgtg ctagattacc aagtgatccg
     2281 tttactcatc tagctcctaa atgcagaacc cgagagttgc ctgatggtac attttattca
     2341 actctttatc tgccaattaa ctcacctctt cgagcctcca ttgttggtcc accaatgagc
     2401 tgtgtacgat tggctgaaag agttgtagct ctcatttgct gtgagaaact gcacaaaatt
     2461 ggcgaactgg atgaccattt gatgccagtt gggaaagaga ctgttaaata tgaagaggag
     2521 cttgatttgc atgatgaaga agagaccagt gttccaggaa gaccaggttc cacgaaacga
     2581 aggcagtgct acccaaaagc aattccagag tgtttgaggg atagttatcc cagacctgat
     2641 cagccctgtt acctgtatgt gataggaatg gttttaacta cacctttacc tgatgaactc
     2701 aactttagaa ggcggaagct ctatcctcct gaagatacca caagatgctt tggaatactg
     2761 acggccaaac ccatacctca gattccacac tttcctgtgt acacacgctc tggagaggtt
     2821 accatatcca ttgagttgaa gaagtctggt ttcatgttgt ctctacaaat gcttgagttg
     2881 attacaagac ttcaccagta tatattctca catattcttc ggcttgaaaa acctgcacta
     2941 gaatttaaac ctacagacgc tgattcagca tactgtgttc tacctcttaa tgttgttaat
     3001 gactccagca ctttggatat tgactttaaa ttcatggaag atattgagaa gtctgaagct
     3061 cgcataggca ttcccagtac aaagtataca aaagaaacac cctttgtttt taaattagaa
     3121 gattaccaag atgccgttat cattccaaga tatcgcaatt ttgatcagcc tcatcgattt
     3181 tatgtagctg atgtgtacac tgatcttacc ccactcagta aatttccttc ccctgagtat
     3241 gaaacttttg cagaatatta taaaacaaag tacaaccttg acctaaccaa tctcaaccag
     3301 ccactgctgg atgtggacca cacatcttca agacttaatc ttttgacacc tcgacatttg
     3361 aatcagaagg ggaaagcgct tcctttaagc agtgctgaga agaggaaagc caaatgggaa
     3421 agtctgcaga ataaacagat actggttcca gaactctgtg ctatacatcc aattccagca
     3481 tcactgtgga gaaaagctgt ttgtctcccc agcatacttt atcgccttca ctgccttttg
     3541 actgcagagg agctaagagc ccagactgcc agcgatgctg gcgtgggagt cagatcactt
     3601 cctgcggatt ttagataccc taacttagac ttcgggtgga aaaaatctat tgacagcaaa
     3661 tctttcatct caatttctaa ctcctcttca gctgaaaatg ataattactg taagcacagc
     3721 acaattgtcc ctgaaaatgc tgcacatcaa ggtgctaata gaacctcctc tctagaaaat
     3781 catgaccaaa tgtctgtgaa ctgcagaacg ttgctcagcg agtcccctgg taagctccac
     3841 gttgaagttt cagcagatct tacagcaatt aatggtcttt cttacaatca aaatctcgcc
     3901 aatggcagtt atgatttagc taacagagac ttttgccaag gaaatcagct aaattactac
     3961 aagcaggaaa tacccgtgca accaactacc tcatattcca ttcagaattt atacagttac
     4021 gagaaccagc cccagcccag cgatgaatgt actctcctga gtaataaata ccttgatgga
     4081 aatgctaaca aatctacctc agatggaagt cctgtgatgg ccgtaatgcc tggtacgaca
     4141 gacactattc aagtgctcaa gggcaggatg gattctgagc agagcccttc tattgggtac
     4201 tcctcaagga ctcttggccc caatcctgga cttattcttc aggctttgac tctgtcaaac
     4261 gctagtgatg gatttaacct ggagcggctt gaaatgcttg gcgactcctt tttaaagcat
     4321 gccatcacca catatctatt ttgcacttac cctgatgcgc atgagggccg cctttcatat
     4381 atgagaagca aaaaggtcag caactgtaat ctgtatcgcc ttggaaaaaa gaagggacta
     4441 cccagccgca tggtggtgtc aatatttgat ccccctgtga attggcttcc tcctggttat
     4501 gtagtaaatc aagacaaaag caacacagat aaatgggaaa aagatgaaat gacaaaagac
     4561 tgcatgctgg cgaatggcaa actggatgag gattacgagg aggaggatga ggaggaggag
     4621 agcctgatgt ggagggctcc gaaggaagag gctgactatg aagatgattt cctggagtat
     4681 gatcaggaac atatcagatt tatagataat atgttaatgg ggtcaggagc ttttgtaaag
     4741 aaaatctctc tttctccttt ttcaaccact gattctgcat atgaatggaa aatgcccaaa
     4801 aaatcctcct taggtagtat gccattttca tcagattttg aggattttga ctacagctct
     4861 tgggatgcaa tgtgctatct ggatcctagc aaagctgttg aagaagatga ctttgtggtg
     4921 gggttctgga atccatcaga agaaaactgt ggtgttgaca cgggaaagca gtccatttct
     4981 tacgacttgc acactgagca gtgtattgct gacaaaagca tagcggactg tgtggaagcc
     5041 ctgctgggct gctatttaac cagctgtggg gagagggctg ctcagctttt cctctgttca
     5101 ctggggctga aggtgctccc ggtaattaaa aggactgatc gggaaaaggc cctgtgccct
     5161 actcgggaga atttcaacag ccaacaaaag aacctttcag tgagctgtgc tgctgcttct
     5221 gtggccagtt cacgctcttc tgtattgaaa gactcggaat atggttgttt gaagattcca
     5281 ccaagatgta tgtttgatca tccagatgca gataaaacac tgaatcacct tatatcgggg
     5341 tttgaaaatt ttgaaaagaa aatcaactac agattcaaga ataaggctta ccttctccag
     5401 gcttttacac atgcctccta ccactacaat actatcactg attgttacca gcgcttagaa
     5461 ttcctgggag atgcgatttt ggactacctc ataaccaagc acctttatga agacccgcgg
     5521 cagcactccc cgggggtcct gacagacctg cggtctgccc tggtcaacaa caccatcttt
     5581 gcatcgctgg ctgtaaagta cgactaccac aagtacttca aagctgtctc tcctgagctc
     5641 ttccatgtca ttgatgactt tgtgcagttt cagcttgaga agaatgaaat gcaaggaatg
     5701 gattctgagc ttaggagatc tgaggaggat gaagagaaag aagaggatat tgaagttcca
     5761 aaggccatgg gggatatttt tgagtcgctt gctggtgcca tttacatgga tagtgggatg
     5821 tcactggaga cagtctggca ggtgtactat cccatgatgc ggccactaat agaaaagttt
     5881 tctgcaaatg taccccgttc ccctgtgcga gaattgcttg aaatggaacc agaaactgcc
     5941 aaatttagcc cggctgagag aacttacgac gggaaggtca gagtcactgt ggaagtagta
     6001 ggaaagggga aatttaaagg tgttggtcga agttacagga ttgccaaatc tgcagcagca
     6061 agaagagccc tccgaagcct caaagctaat caacctcagg ttcccaatag ctgaaaccgc
     6121 tttttaaaat tcaaaacaag aaacaaaaca aaaaaaatta aggggaaaat tatttaaatc
     6181 ggaaaggaag acttaaagtt gttagtgagt ggaatgaatt gaaggcagaa tttaaagttt
     6241 ggttgataac aggatagata acagaataaa acatttaaca tatgtataaa attttggaac
     6301 taattgtagt tttagttttt tgcgcaaaca caatcttatc ttctttcctc acttctgctt
     6361 tgtttaaatc acaagagtgc tttaatgatg acatttagca agtgctcaaa ataattgaca
     6421 ggttttgttt tttttttttt gagtttatgt cagctttgct tagtgttaga aggccatgga
     6481 gcttaaacct ccagcagtcc ctaggatgat gtagattctt ctccatctct ccgtgtgtgc
     6541 agtagtgcca gtcctgcagt agttgataag ctgaatagaa agataaggtt ttcgagagga
     6601 gaagtgcgcc aatgttgtct tttctttcca cgttatactg tgtaaggtga tgttcccggt
     6661 cgctgttgca cctgatagta agggacagat ttttaatgaa cattggctgg catgttggtg
     6721 aatcacattt tagttttctg atgccacata gtcttgcata aaaaagggtt cttgccttaa
     6781 aagtgaaacc ttcatggata gtctttaatc tctgatcttt ttggaacaaa ctgttttaca
     6841 ttcctttcat tttattatgc attagacgtt gagacagcgt gatacttaca actcactagt
     6901 atagttgtaa cttattacag gatcatacta aaatttctgt catatgtata ctgaagacat
     6961 tttaaaaacc agaatatgta gtctacggat attttttatc ataaaaatga tctttggcta
     7021 aacaccccat tttactaaag tcctcctgcc aggtagttcc cactgatgga aatgtttatg
     7081 gcaaataatt ttgccttcta ggctgttgct ctaacaaaat aaaccttaga catatcacac
     7141 ctaaaatatg ctgcagattt tataattgat tggttactta tttaagaagc aaaacacagc
     7201 acctttaccc ttagtctcct cacataaatt tcttactata cttttcataa tgttgcatgc
     7261 atatttcacc taccaaagct gtgctgttaa tgccgtgaaa gtttaacgtt tgcgataaac
     7321 tgccgtaatt ttgatacatc tgtgatttag gtcattaatt tagataaact agctcattat
     7381 ttccatcttt ggaaaaggaa aaaaaaaaaa acttctttag gcatttgcct aagtttcttt
     7441 aattagactt gtaggcactc ttcacttaaa tacctcagtt cttcttttct tttgcatgca
     7501 tttttcccct gtttggtgct atgtttatgt attatgcttg aaattttaat tttttttttt
     7561 ttgcactgta actataatac ctcttaattt acctttttaa aagctgtggg tcagtcttgc
     7621 actcccatca acataccagt agaggtttgc tgcaatttgc cccgttaatt atgcttgaag
     7681 tttaagaaag ctgagcagag gtgtctcata tttcccagca catgattctg aacttgatgc
     7741 ttcgtggaat gctgcattta tatgtaagtg acatttgaat actgtccttc ctgctttatc
     7801 tgcatcatcc acccacagag aaatgcctct gtgcgagtgc accgacagaa aactgtcagc
     7861 tctgctttct aaggaaccct gagtgagggg ggtattaagc ttctccagtg ttttttgttg
     7921 tctccaatct taaacttaaa ttgagatcta aattattaaa cgagtttttg agcaaattag
     7981 gtgacttgtt ttaaaaatat ttaattccga tttggaacct tagatgtcta tttgattttt
     8041 taaaaaacct taatgtaaga tatgaccagt taaaacaaag caattcttga attatataac
     8101 tgtaaaagtg tgcagttaac aaggctggat gtgaatttta ttctgagggt gatttgtgat
     8161 caagtttaat cacaaatctc ttaatattta taaactacct gatgccagga gcttagggct
     8221 ttgcattgtg tctaatacat tgatcccagt gttacgggat tctcttgatt cctggcacca
     8281 aaatcagatt gttttcacag ttatgattcc cagtgggaga aaaatgcctc aatatatttg
     8341 taaccttaag aagagtattt ttttgttaat actaagatgt tcaaacttag acatgattag
     8401 gtcatacatt ctcaggggtt caaatttcct tctaccattc aaatgtttta tcaacagcaa
     8461 acttcagccg tttcactttt tgttggagaa aaatagtaga ttttaatttg actcacagtt
     8521 tgaagcattc tgtgatcccc tggttactga gttaaaaaat aaaaaagtac gagttagaca
     8581 tatgaaatgg ttatgaacgc ttttgtgctg ctgattttta atgctgtaaa gttttcctgt
     8641 gtttagcttg ttgaaatgtt ttgcatctgt caattaagga aaaaaaaaat cactctatgt
     8701 tgccccactt tagagccctg tgtgccaccc tgtgttcctg tgattgcaat gtgagaccga
     8761 atgtaatatg gaaaacctac cagtggggtg tggttgtgcc ctgagcacgt gtgtaaagga
     8821 ctggggaggc gtgtcttgaa aaagcaactg cagaaattcc ttatgatgat tgtgtgcaag
     8881 ttagttaaca tgaaccttca tttgtaaatt ttttaaaatt tcttttataa tatgctttcc
     8941 gcagtcctaa ctatgctgcg ttttataata gctttttccc ttctgttctg ttcatgtagc
     9001 acagataagc attgcacttg gtaccatgct ttacctcatt tcaagaaaat atgcttaaca
     9061 gagaggaaaa aaatgtggtt tggccttgct gctgttttga tttatggaat ttgaaaaaga
     9121 taattataat gcctgcaatg tgtcatatac tcgcacaact taaataggtc atttttgtct
     9181 gtggcatttt tactgtttgt gaaagtatga aacagatttg ttaactgaac tcttaattat
     9241 gtttttaaaa tgtttgttat atttcttttc ttttttcttt tatattacgt gaagtgatga
     9301 aatttagaat gacctctaac actcctgtaa ttgtctttta aaatactgat atttttattt
     9361 gttaataata ctttgccctc agaaagattc tgataccctg ccttgacaac atgaaacttg
     9421 aggctgcttt ggttcatgaa tccaggtgtt cccccggcag tcggcttctt cagtcgctcc
     9481 ctggaggcag gtgggcactg cagaggatca ctggaatcca gatcgagcgc agttcatgca
     9541 caaggccccg ttgatttaaa atattggatc ttgctctgtt agggtgtcta atccctttac
     9601 acaagattga agccaccaaa ctgagacctt gatacctttt tttaactgca tctgaaatta
     9661 tgttaagagt ctttaaccca tttgcattat ctgcagaaga gaaactcatg tcatgtttat
     9721 tacctatatg gttgttttaa ttacatttga ataattatat ttttccaacc actgattact
     9781 tttcaggaat ttaattattt ccagataaat ttctttattt tatattgtac atgaaaagtt
     9841 ttaaagatat gtttaagacc aagactatta aaatgatttt taaagttgtt ggagacgcca
     9901 atagcaatat ctaggaaatt tgcattgaga ccattgtatt ttccactagc agtgaaaatg
     9961 atttttcaca actaacttgt aaatatattt taatcattac ttcttttttt ctagtccatt
    10021 tttatttgga catcaaccac agacaattta aattttatag atgcactaag aattcactgc
    10081 agcagcaggt tacatagcaa aaatgcaaag gtgaacagga agtaaatttc tggcttttct
    10141 gctgtaaata gtgaaggaaa attactaaaa tcaagtaaaa ctaatgcata ttatttgatt
    10201 gacaataaaa tatttaccat cacatgctgc agctgttttt taaggaacat gatgtcattc
    10261 attcatacag taatcatgct gcagaaattt gcagtctgca ccttatggat cacaattacc
    10321 tttagttgtt ttttttgtaa taattgtagc caagtaaatc tccaataaag ttatcgtctg
    10381 ttca
//
