LOCUS       NM_006729               9203 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens diaphanous related formin 2 (DIAPH2), transcript
            variant 156, mRNA.
ACCESSION   NM_006729
VERSION     NM_006729.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Cichocka,E., Gorczynska-Kosiorz,S., Misiolek,M., Scierski,W.,
            Koniewska,A., Stryjewska-Makuch,G. and Gumprecht,J.
  TITLE     DIAPH2 gene polymorphisms and laryngeal cancer risk in men
  JOURNAL   J Cancer Res Clin Oncol 150 (6), 295 (2024)
   PUBMED   38844723
  REMARK    GeneRIF: DIAPH2 gene polymorphisms and laryngeal cancer risk in
            men.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Snit,M., Misiolek,M., Scierski,W., Koniewska,A.,
            Stryjewska-Makuch,G., Okla,S. and Grzeszczak,W.
  TITLE     DIAPH2, PTPRD and HIC1 Gene Polymorphisms and Laryngeal Cancer Risk
  JOURNAL   Int J Environ Res Public Health 18 (14), 7486 (2021)
   PUBMED   34299935
  REMARK    GeneRIF: DIAPH2, PTPRD and HIC1 Gene Polymorphisms and Laryngeal
            Cancer Risk.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9203)
  AUTHORS   Kostrzewska-Poczekaj,M., Byzia,E., Soloch,N., Jarmuz-Szymczak,M.,
            Janiszewska,J., Kowal,E., Paczkowska,J., Kiwerska,K.,
            Wierzbicka,M., Bartochowska,A., Ustaszewski,A., Greczka,G.,
            Grenman,R., Szyfter,K. and Giefing,M.
  TITLE     DIAPH2 alterations increase cellular motility and may contribute to
            the metastatic potential of laryngeal squamous cell carcinoma
  JOURNAL   Carcinogenesis 40 (10), 1251-1259 (2019)
   PUBMED   30793164
  REMARK    GeneRIF: Low DIAPH2 expression is associated with metastasis of
            laryngeal squamous cell carcinoma.
REFERENCE   4  (bases 1 to 9203)
  AUTHORS   Tabibnejad,N., Sheikhha,M.H., Ghasemi,N., Fesahat,F., Soleimani,M.
            and Aflatoonian,A.
  TITLE     Association between early embryo morphokinetics plus cumulus cell
            gene expression and assisted reproduction outcomes in polycystic
            ovary syndrome women
  JOURNAL   Reprod Biomed Online 38 (2), 139-151 (2019)
   PUBMED   30593440
  REMARK    GeneRIF: DIAPH2 gene expression was an independent prognostic
            factors of clinical pregnancy rate and live birth in ICSI
            pregnancies.
REFERENCE   5  (bases 1 to 9203)
  AUTHORS   Dvorak,K.M., Pettee,K.M., Rubinic-Minotti,K., Su,R.,
            Nestor-Kalinoski,A. and Eisenmann,K.M.
  TITLE     Carcinoma associated fibroblasts (CAFs) promote breast cancer
            motility by suppressing mammalian Diaphanous-related formin-2
            (mDia2)
  JOURNAL   PLoS One 13 (3), e0195278 (2018)
   PUBMED   29596520
  REMARK    GeneRIF: Low DIA2 expression is associated with breast cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9203)
  AUTHORS   Satoh,S. and Tominaga,T.
  TITLE     mDia-interacting protein acts downstream of Rho-mDia and modifies
            Src activation and stress fiber formation
  JOURNAL   J Biol Chem 276 (42), 39290-39294 (2001)
   PUBMED   11509578
REFERENCE   7  (bases 1 to 9203)
  AUTHORS   Bione,S., Sala,C., Manzini,C., Arrigo,G., Zuffardi,O., Banfi,S.,
            Borsani,G., Jonveaux,P., Philippe,C., Zuccotti,M., Ballabio,A. and
            Toniolo,D.
  TITLE     A human homologue of the Drosophila melanogaster diaphanous gene is
            disrupted in a patient with premature ovarian failure: evidence for
            conserved function in oogenesis and implications for human
            sterility
  JOURNAL   Am J Hum Genet 62 (3), 533-541 (1998)
   PUBMED   9497258
REFERENCE   8  (bases 1 to 9203)
  AUTHORS   Volorio,S., Simon,G., Repetto,M., Cucciardi,M., Banfi,S.,
            Borsani,G., Ballabio,A. and Zollo,M.
  TITLE     Sequencing analysis of forty-eight human image cDNA clones similar
            to Drosophila mutant protein
  JOURNAL   DNA Seq 9 (5-6), 307-315 (1998)
   PUBMED   10524757
REFERENCE   9  (bases 1 to 9203)
  AUTHORS   Sala,C., Arrigo,G., Torri,G., Martinazzi,F., Riva,P., Larizza,L.,
            Philippe,C., Jonveaux,P., Sloan,F., Labella,T. and Toniolo,D.
  TITLE     Eleven X chromosome breakpoints associated with premature ovarian
            failure (POF) map to a 15-Mb YAC contig spanning Xq21
  JOURNAL   Genomics 40 (1), 123-131 (1997)
   PUBMED   9070928
REFERENCE   10 (bases 1 to 9203)
  AUTHORS   Philippe,C., Cremers,F.P., Chery,M., Bach,I., Abbadi,N.,
            Ropers,H.H. and Gilgenkrantz,S.
  TITLE     Physical mapping of DNA markers in the q13-q22 region of the human
            X chromosome
  JOURNAL   Genomics 17 (1), 147-152 (1993)
   PUBMED   8406446
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Y15909.1, U69179.1, DB062196.1
            and AL391821.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006729.4.
            
            Summary: The product of this gene belongs to the diaphanous
            subfamily of the formin homology family of proteins. This gene may
            play a role in the development and normal function of the ovaries.
            Defects in this gene have been linked to premature ovarian failure
            2. Alternatively spliced transcript variants encoding different
            isoforms have been identified. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (156) represents the longer
            transcript, and encodes the longer isoform (isoform 156).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y15909.1, SRR1803612.128120.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324765.13/ ENSP00000321348.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3848              Y15909.1           134-3981
            3849-3883           Y15909.1           3983-4017
            3884-3891           U69179.1           1460-1467
            3892-4080           Y15909.1           4026-4214
            4081-4358           Y15909.1           4216-4493
            4359-4472           Y15909.1           4495-4608
            4473-4804           DB062196.1         189-520
            4805-9203           AL391821.7         53918-58316         c
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq21.33"
     gene            1..9203
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /note="diaphanous related formin 2"
                     /db_xref="GeneID:1730"
                     /db_xref="HGNC:HGNC:2877"
                     /db_xref="MIM:300108"
     exon            1..349
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..3523
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /note="isoform 156 is encoded by transcript variant 156;
                     diaphorase-2; diaphanous homolog 2; Premature ovarian
                     failure 2A"
                     /codon_start=1
                     /product="protein diaphanous homolog 2 isoform 156"
                     /protein_id="NP_006720.1"
                     /db_xref="CCDS:CCDS14467.1"
                     /db_xref="GeneID:1730"
                     /db_xref="HGNC:HGNC:2877"
                     /db_xref="MIM:300108"
                     /translation="MEQPGAAASGAGGGSEEPGGGRSNKRSAGNRAANEEETKNKPKL
                     NIQIKTLADDVRDRITSFRKSTVKKEKPLIQHPIDSQVAMSEFPAAQPLYDERSLNLS
                     EKEVLDLFEKMMEDMNLNEEKKAPLRNKDFTTKREMVVQYISATAKSGGLKNSKHECT
                     LSSQEYVHELRSGISDEKLLNCLESLRVSLTSNPVSWVNNFGHEGLGLLLDELEKLLD
                     KKQQENIDKKNQYKLIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQPNMMTEIV
                     KILSAICIVGEENILDKLLGAITTAAERNNRERFSPIVEGLENQEALQLQVACMQFIN
                     ALVTSPYELDFRIHLRNEFLRSGLKTMLPDLKEKENDELDIQLKVFDENKEDDLTELS
                     HRLNDIRAEMDDMNEVYHLLYNMLKDTAAENYFLSILQHFLLIRNDYYIRPQYYKIIE
                     ECVSQIVLHCSGMDPDFKYRQRLDIDLTHLIDSCVNKAKVEESEQKAAEFSKKFDEEF
                     TARQEAQAELQKRDEKIKELEAEIQQLRTQAQVLSSSSGIPGPPAAPPLPGVGPPPPP
                     PAPPLPGGAPLPPPPPPLPGMMGIPPPPPPPLLFGGPPPPPPLGGVPPPPGISLNLPY
                     GMKQKKMYKPEVSMKRINWSKIEPTELSENCFWLRVKEDKFENPDLFAKLALNFATQI
                     KVQKNAEALEEKKTGPTKKKVKELRILDPKTAQNLSIFLGSYRMPYEDIRNVILEVNE
                     DMLSEALIQNLVKHLPEQKILNELAELKNEYDDLCEPEQFGVVMSSVKMLQPRLSSIL
                     FKLTFEEHINNIKPSIIAVTLACEELKKSESFNRLLELVLLVGNYMNSGSRNAQSLGF
                     KINFLCKIRDTKSADQKTTLLHFIADICEEKYRDILKFPEELEHVESASKVSAQILKS
                     NLASMEQQIVHLERDIKKFPQAENQHDKFVEKMTSFTKTAREQYEKLSTMHNNMMKLY
                     ENLGEYFIFDSKTVSIEEFFGDLNNFRTLFLEAVRENNKRREMEEKTRRAKLAKEKAE
                     QEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRVPLE
                     RSRSRHNGAISSK"
     exon            350..382
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            383..559
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            560..664
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            665..804
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            805..879
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            880..949
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1086
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1195
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1306
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1425
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1542
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1661
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1726
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1831
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..2152
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2267
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2369
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2464
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2566
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..2806
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2936
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2937..3061
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3062..3226
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3227..3362
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3363..3458
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..9203
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4782..4787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /note="hexamer: AATAAA"
     polyA_site      4804
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /note="major polyA site"
     regulatory      9180..9185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
                     /note="hexamer: AATAAA"
     polyA_site      9203
                     /gene="DIAPH2"
                     /gene_synonym="DIA; DIA2; DRF2; POF; POF2; POF2A"
ORIGIN      
        1 gccccagcgc cgagtagtgg caacggcgtg gttgcgtcgg gggtgcctgg gagcctggag
       61 tcccgggggc ctgaaatcgg cagcttcccg ggcagacact ctctccctca ggaagaggtg
      121 ccgccgagtc agcgcggggc agtgtgagcg ccccgaggtg ctttctcagt tgaggagagg
      181 tggggttaca gggcacaggt gacagggccg gagaaagatg gagcagcccg gggcggcggc
      241 gtcgggagcg ggaggcggca gcgaggaacc cggtgggggc cggagcaaca agcggagcgc
      301 ggggaaccgg gccgccaatg aagaggaaac gaaaaacaaa cccaaattga acattcaaat
      361 aaaaactttg gcagatgatg tgcgtgaccg aattacaagt tttagaaaat ctactgtcaa
      421 aaaagaaaaa cctcttattc aacatcctat tgattctcaa gtcgcgatga gtgagtttcc
      481 tgcagctcag ccattatatg atgaacgatc tttgaatttg tcagaaaagg aagtattgga
      541 tctctttgaa aaaatgatgg aggacatgaa ccttaacgaa gagaaaaaag ctcctttacg
      601 aaacaaagac tttaccacca aacgtgagat ggttgtccag tatatttctg ccactgccaa
      661 atctggtggg ctgaaaaaca gcaaacatga atgcaccctg tcttcacaag aatatgttca
      721 tgaattacga tcgggtatat cagatgagaa acttcttaat tgcctagaat ccctcagggt
      781 ttctttaacc agcaatccgg tcagctgggt taacaacttt ggccatgaag gtcttggact
      841 cttattggat gagctggaaa agcttctgga caaaaaacag caagaaaata ttgacaagaa
      901 gaatcagtat aaacttattc aatgcctcaa agcatttatg aataataagt ttggattaca
      961 aaggattcta ggagatgaaa gaagtctttt actattggca agagcaattg accccaaaca
     1021 acccaacatg atgactgaaa tagtaaaaat actttctgct atttgcattg ttggagaaga
     1081 gaacattcta gataaacttt taggggctat aacaacagca gcagaaagaa ataacaggga
     1141 acgattttca ccaattgtgg aaggtttaga aaatcaggaa gccttgcaat tacaggtggc
     1201 ctgcatgcag tttataaatg cccttgtcac ttctccttat gagcttgatt ttcgaataca
     1261 tttaaggaat gaattcctcc gttcaggact aaaaacaatg ttaccagatc taaaagaaaa
     1321 agagaatgat gagcttgata ttcagttgaa agtatttgat gaaaacaaag aagatgacct
     1381 aactgaatta tcacaccgtc tcaatgacat tcgagcagaa atggatgata tgaatgaagt
     1441 ctaccatctt ctatataata tgctgaagga cactgctgct gaaaattact tcttatctat
     1501 tctacaacat tttttgctta tcagaaatga ttattatatc aggccacaat attataaaat
     1561 aattgaggaa tgtgtttcac agatagtgct acactgcagt ggtatggatc cagacttcaa
     1621 atacaggcaa agattagaca tcgatttaac tcatctgata gattcttgtg tgaacaaggc
     1681 gaaagttgaa gaaagtgaac aaaaagctgc agagttttca aagaagttcg atgaagaatt
     1741 cacagctcga caggaagctc aagcagagct tcaaaaaaga gatgagaaaa tcaaagaact
     1801 tgaagcagaa atccagcaac ttcgaaccca ggcacaagta ctctcaagtt catcaggaat
     1861 tccaggtcct cctgcagcac ctccattgcc aggtgtaggg ccgcctccac caccacccgc
     1921 gccacctcta cccggaggag ctcctcttcc tcctccacca cctcctttac ctggaatgat
     1981 ggggatacca ccaccacccc caccaccact tttatttggg ggacctcctc caccaccacc
     2041 ccttggagga gttcctcctc ccccaggaat atcacttaat ctaccttatg gaatgaagca
     2101 gaaaaaaatg tataaacctg aagtgtccat gaagagaatc aattggtcaa agattgaacc
     2161 cacagaatta tctgagaact gtttctggtt aagagtcaaa gaagacaagt ttgagaatcc
     2221 agatctcttt gccaaattgg cattgaattt tgctactcag ataaaagttc aaaagaacgc
     2281 agaagcatta gaagaaaaga agactgggcc tacaaagaag aaagtgaaag aactgagaat
     2341 tttggatccc aaaacagctc agaatctgtc catctttctg ggatcatatc gcatgccata
     2401 tgaagacata agaaacgtta ttctggaggt taatgaagac atgctgagtg aggctttaat
     2461 tcagaacctt gtgaaacatc ttcctgagca gaagatactc aacgaattag cagagcttaa
     2521 gaatgaatat gatgacctct gtgagcctga acaatttgga gttgtgatga gctctgtgaa
     2581 aatgttacag cctcgtctca gtagtatcct gttcaagctc acatttgaag aacacataaa
     2641 caacatcaaa ccaagcatca tagcagtaac tcttgcctgt gaagaactga agaaaagtga
     2701 aagctttaac agacttttag agttagttct tcttgttgga aactacatga actcaggctc
     2761 aagaaatgcc cagtctttgg gatttaagat caacttcctt tgtaagatca gagatactaa
     2821 atcagcggat caaaaaacaa cccttttgca ttttattgcc gacatttgtg aggaaaaata
     2881 tcgagatatc ctaaaatttc ctgaagaact ggaacacgta gaaagtgcaa gcaaagtttc
     2941 agctcaaatt ctcaagagca accttgcatc aatggaacaa caaattgttc atctggaacg
     3001 tgacatcaag aaattccccc aagcagaaaa tcaacacgat aagtttgtgg aaaagatgac
     3061 cagctttaca aagactgccc gagaacagta tgaaaaactc tccaccatgc acaacaacat
     3121 gatgaagctc tatgagaatc ttggagaata cttcattttt gactcaaaga cagtgagcat
     3181 agaagagttc tttggtgatc tcaacaactt ccgaactttg tttttggaag cagtgagaga
     3241 aaacaataag agaagagaaa tggaagagaa gaccaggagg gcaaaacttg caaaagagaa
     3301 agctgaacaa gaaaagttag aacgccagaa gaaaaagaaa caactcattg atataaacaa
     3361 agagggtgat gagactggtg tgatggataa tcttctagaa gccctacaat caggtgcagc
     3421 attcagagac cgtcgaaagc ggattccaag gaatccagat aacagacgag tacctttgga
     3481 aaggtcacgc tctcgccaca atggagctat ctcatctaag tgattcctga tgccaaagaa
     3541 tatgaaaata tttaagtaaa aacaaaatga tgcattttga gaagaacaaa gtgtgcactc
     3601 agcggctgga aaggaaataa gtgcatttct gcaaagatca agataagctg gatgaagtag
     3661 tgtgcatttg taatactatt gcaagactcc tcccacaatt attctaatct gaacacagtt
     3721 atcaggatta caaaatgtgt tccattttag tgtaaagatg tgtatcattt gtaattgtgt
     3781 gtgacctgac tgtgatgatg gaagtgtaag aaattgaaga gttctaaggc tttcaaaaac
     3841 actcagtcaa aaatttaaaa tcttaaatgt gtaagtcacc cccaccaaaa acaaaagaga
     3901 agaaaaagaa tagaaagaat tatccagtgt cagcttccaa tccctagtac atatataata
     3961 gaaaagggcc aagaaaaata tgcatcttga tttggagaga ggctaatatt atgcacactg
     4021 taagagaagc cattttggaa attcacgaaa gtactgctcc accccagttg agttatttag
     4081 aattttatct caagtgaaag ctgatggatt catctgcttt ggctgaaatt aaacttatca
     4141 ttagtctagc tagcatttca gcatgatatt gcaagcactt ctcattgcta aaaataaata
     4201 aaccaaagtt taaccgaatc agttagggaa agtgatttaa actttattta aagaggtatt
     4261 ttctaattat gcacagatat ctactttata caaatacttt atatggctat ttttgagaaa
     4321 accctcacat tttaatgttt atgctaggga tgaacctgaa aattctatta cgtttattta
     4381 gatttcaaag gcaaatattg attcctatgc tctgtggttt atttcttttt tctattgctt
     4441 ctttctccct tgagtccctt gaaggcaggg aatagacttc tagaaaacct gagaggaaaa
     4501 agaattcttt ttacaggagg cagcagaaaa ctgtctgaaa ggtcaattgt tttatctccc
     4561 tttccactct ctttccaatt tcactttggt ggtctgaaga agaaaaagaa attttatgta
     4621 tgtatgtgta aatatgtgta tatatttcta tctcttgcta caataattcc aactaagtga
     4681 acttctcaat tatcatcata cttacttacc ttatattaac aaattaagat gatgctgcca
     4741 aaacaagtct agcagggaaa acaggttcta catttttctt aaataaatta gggagtaaaa
     4801 gttatactta acttgtctgt ctattatttt aaaatatgca ttgaaataat gtggtataac
     4861 ttctctggag tgcaatttta agtctaattc atgcagatga ttgattgtga ttattgtaag
     4921 aaatttctta catgtatatt tcttatgtag aaaatactgt ttctactcat tcaaagcaca
     4981 ttgtagatat tcagagagaa gaacaaatgg ctggcttggc aaatccccat ctgagataaa
     5041 gtaaacaagt gaccagcagc ccacagatga ttcctttata tagatgtata atgattcaaa
     5101 ctgctgcctt tgcctccagt gcatccttac ttaggtatta tacttcttta aaaagctctg
     5161 aagctgctac aatagaaaaa tcaaaaaggg taacagtatc tgaaatttca cagtcctatc
     5221 cgatctgaaa acacacatac acaaatgcac acacatgcac acacacattt aagggacata
     5281 gcagtaacac tgttttttaa caacaacaaa agtttaggtt ctaatttatg taaataccta
     5341 gtatgtgtat ttttgacttt acaaagtctt tcttttctga aatcttcgtg gtatggcttt
     5401 tttaattctt ttgacagctt atcagtctgt aggagaatga tcttgaaatg ttaaccctga
     5461 ctaaaatttg ggagcatatg ccttgctata ttatcaagct ggtcaaggta gaaatactga
     5521 tatgtatcat caccttttcc agatacaaac ttgccttatt ttctagtttg tgaacaagaa
     5581 cgaatgaagt actattattg ataacttata tatttttatt tagagcaatc ccacatgctt
     5641 ttcacaaaca ttaaaaaaaa aaacctttca gggtctttca tgaagttcca atagttgagt
     5701 gagttaaaag gtttgtttcc cagatttgta atggtcctgc tctagccatt tgcagttttg
     5761 ttttagatga ttggaaagta tatcaccaaa aaaagtatat agtgacattt ttagtatgaa
     5821 accaaattac actaaggtct tttgttggcc atattactgc cacacatgag aagcaccatt
     5881 tatgcagatt ttcttcagaa tatttttaaa aggtacagaa tccttaaaat attaacaact
     5941 aattgcatcc atcatctcct ttggctgctg taacaaatta ccaccaataa tatgatggct
     6001 taacacaaca gaaacttatt tgcttacatt tttgaagggc agcaaagcca tgctccctcc
     6061 agaggctcta gaggagaatc tttccttgcc acgtccacct tttggtggct attggcattt
     6121 tttggcttat ggccacattt ctctttctgc tgtgtcttca catcaccctc ctctgtatac
     6181 ctaatctgcc tctgcctctg tttaataagg atccttatga tgacttttag ggactaccca
     6241 gattactcca ggataatctc ctcacctcat gagtcttaac ttaattacat ctgcaaggcc
     6301 cccttctcca aataagataa attcatggtt ttggggatta ggatgtggct atgtctttta
     6361 ggaagccact cttagcccac cagagtctgc cctttggccc aaaatttatg tctgtctcat
     6421 gtgtaaaaga cattcatccc atcctaacat taccaaaagt ctcaacccat tatatcaatc
     6481 ctaagtccaa aatttcattc aaatatcatc ggttcaaaag ttccaaagct tatcatttag
     6541 atcatctaaa tcaggtatga gtgaaactct gggtatggtc tttcctgagg ccaaattcct
     6601 cttcctctga gaaccagtga aacagaaaag ttaatctgct ttcaaattac aatggtggga
     6661 acaggcatag gataacagtt acagacattt ctgtctcaaa aaggagagaa tggaaattgg
     6721 aatggagtct cctgtcccaa tcattttcaa aatctagcca ggcaaatttc tgttatattt
     6781 caaggcctgg cagtaatcct ctttctcagg gctccaccct ttgggcctgt agctcttctt
     6841 tcttggacta tggattgggc ctctgcctct gtgcctatgg gttgcacttc ttcctctgtt
     6901 cccacacttg ggcctctgcc tctgtacctg cagctctgct ctcgggatgg tgcttccttt
     6961 atcttgaagg gtggcacatg tttgcagctg agaagtttta tcaacgggtt tcctacctgt
     7021 agaattctgg cagtctcaca atcttctttc atttttttct gtctctgtcc cttttaggcc
     7081 cagttggaag tgtttctgat ggtagaacat tctcaaaaat cttgtgggtc ttctacgtat
     7141 gtcacattga ttcacacatt cagaaaagag ggtcctcaca gatttttcct gaacaacccc
     7201 atctctattc ttggcttctg ttgaaacagt caaataaatc cacaagttaa tgcctaacct
     7261 cttcagcact agccaaaggt tgtcaagaca cacccttggg cttatctaca gagcacactt
     7321 ttctaacagt gaatctccta attttagcat cttttttttt ttttacaatc attcagattg
     7381 tgaattcagg ctcagaattt acccagtcat cagatactga ttccttttgt ccacttatat
     7441 agttattttc tctttaaaaa aaaatttttt ttaatgtatt agagatgagg tctcactctg
     7501 tcacccaggc tgcagtgtag tggtgcaaac atagctccct gtaacctcaa actcccaggc
     7561 tcaagggatc ctcctgcatc agccttccaa gtagctgggt ctacaggtgc atgccaccat
     7621 gcctggctaa ttttttgcat ttttttgtag agacttgatc ttgctatgtt gtccaggctg
     7681 ctctcgaact cctagcctca agcaatccac ccatcttggc ctcctaaagt gccggattac
     7741 aggtgtaagc caccatgccc agcctgtttt gtcaatgtat ctgttttctg tcttatttta
     7801 ctataagcac taagaagaaa ctaagctata tctctaacac tagacttgaa aatttcctca
     7861 gctaaacatc caagttcatc atttattcaa cttttcacac tactgtagta gacaatttag
     7921 atagataaac tttttgccac tatatgacaa ggattccctt tcttccattt tccaataaaa
     7981 tcttcatttc cttctaaacc atcactagaa atgcctttaa cattcatact actaccatct
     8041 ggtaaaggca atctagtttt ttctatcaca gccaccaaaa ttcttctagt ctctacccat
     8101 tatccaattc caaagccttt ttcacatttt aagacatttg ttacagaagt acccaatccg
     8161 tcccagttcc acaatctgca ttagattccc atggctgctg taacaaatta ccctctagcc
     8221 cagtggctta aaacaataga aatttattat cttgttgttt tggaggccaa aagtccaaaa
     8281 ttgaggggtt ggcagggctg aacgtcttct ggagactcta agggaaacac tcttcccgtg
     8341 tcttccagtt tcttgtggct gccatcattc cttggtttgt gactgcatct ccctctcctc
     8401 tgtcttcaca tcacttcccc tctgtatata taatctacct ctgcctctct cttataagga
     8461 cacttgtgac gggacttagg gcccatccag attacccatg ataattccct tattccaaga
     8521 ttcttaatta tatctgaaag gacctttttt ccaaataagg tactatcaca ggttccaggg
     8581 agtaggatat tgaatatctt ttttggggag ggggcaccat gcagctcact acactattca
     8641 ttgcacacaa atgaattttt cactttttaa gatgcattct tggtgctcaa accagatcga
     8701 agtttgtctc taaaagctat tgtctgcaca ggctgctgca tgctctgttg ttaaatggat
     8761 ggacaggcta ttctaaattt tggttgatac ttttgctact atgggcaatt aacttgaaaa
     8821 aaataatcga tcccaactct gtgctctgat gtacctcttc tgcccctttt atgacacctt
     8881 tgaccaaatg ccttctatgg ttcacagtgc aggcacaaaa ctacctctga tacagaaggg
     8941 ttctttacaa gcttatttta cataccgtga atccctcacc taaagggaga ggtgaaagca
     9001 aagactgctt tgaatgggta ttgagggaga ttgtgtccat accaagccac cctgaagaag
     9061 tatttcactt gcagtagaac tgtggatttg tgctgtcatt tcaccttgga ataaacacct
     9121 atctctaagc aggaccaaga atgacttgca atctatatgt aatggctact tacttattca
     9181 ataaagttaa gatatacgtt aaa
//
