LOCUS       NM_005219               5735 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens diaphanous related formin 1 (DIAPH1), transcript
            variant 1, mRNA.
ACCESSION   NM_005219
VERSION     NM_005219.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Azizoglu,Z.B., Babayeva,R., Haskologlu,Z.S., Acar,M.B.,
            Ayaz-Guner,S., Okus,F.Z., Alsavaf,M.B., Can,S., Basaran,K.E.,
            Canatan,M.F., Ozcan,A., Erkmen,H., Leblebici,C.B., Yilmaz,E.,
            Karakukcu,M., Kose,M., Canoz,O., Ozen,A., Karakoc-Aydiner,E.,
            Ceylaner,S., Gumus,G., Per,H., Gumus,H., Canatan,H., Ozcan,S.,
            Dogu,F., Ikinciogullari,A., Unal,E., Baris,S. and Eken,A.
  TITLE     DIAPH1-Deficiency is Associated with Major T, NK and ILC Defects in
            Humans
  JOURNAL   J Clin Immunol 44 (8), 175 (2024)
   PUBMED   39120629
  REMARK    GeneRIF: DIAPH1-Deficiency is Associated with Major T, NK and ILC
            Defects in Humans.
            Erratum:[J Clin Immunol. 2024 Nov 16;45(1):43. doi:
            10.1007/s10875-024-01832-4.. PMID: 39547966]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Palmer,N.J., Barrie,K.R. and Dominguez,R.
  TITLE     Mechanisms of actin filament severing and elongation by formins
  JOURNAL   Nature 632 (8024), 437-442 (2024)
   PUBMED   38843827
  REMARK    GeneRIF: Mechanisms of actin filament severing and elongation by
            formins.
REFERENCE   3  (bases 1 to 5735)
  AUTHORS   Theophall,G.G., Ramirez,L.M.S., Premo,A., Reverdatto,S.,
            Manigrasso,M.B., Yepuri,G., Burz,D.S., Ramasamy,R., Schmidt,A.M.
            and Shekhtman,A.
  TITLE     Disruption of the productive encounter complex results in
            dysregulation of DIAPH1 activity
  JOURNAL   J Biol Chem 299 (11), 105342 (2023)
   PUBMED   37832872
  REMARK    GeneRIF: Disruption of the productive encounter complex results in
            dysregulation of DIAPH1 activity.
REFERENCE   4  (bases 1 to 5735)
  AUTHORS   Yepuri,G., Ramirez,L.M., Theophall,G.G., Reverdatto,S.V.,
            Quadri,N., Hasan,S.N., Bu,L., Thiagarajan,D., Wilson,R., Diez,R.L.,
            Gugger,P.F., Mangar,K., Narula,N., Katz,S.D., Zhou,B., Li,H.,
            Stotland,A.B., Gottlieb,R.A., Schmidt,A.M., Shekhtman,A. and
            Ramasamy,R.
  TITLE     DIAPH1-MFN2 interaction regulates mitochondria-SR/ER contact and
            modulates ischemic/hypoxic stress
  JOURNAL   Nat Commun 14 (1), 6900 (2023)
   PUBMED   37903764
  REMARK    GeneRIF: DIAPH1-MFN2 interaction regulates mitochondria-SR/ER
            contact and modulates ischemic/hypoxic stress.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5735)
  AUTHORS   Mohseni,M., Mohammadi,Y., Zare Ashrafi,F., Ghodratpour,F.,
            Jalalvand,K., Arzhangi,S., Babanejad,M., Azizi,M.H., Kahrizi,K. and
            Najmabadi,H.
  TITLE     An Extended Iranian Family with Autosomal Dominant Non-syndromic
            Hearing Loss Associated with A Nonsense Mutation in the DIAPH1 Gene
  JOURNAL   Arch Iran Med 26 (3), 176-180 (2023)
   PUBMED   37543941
  REMARK    GeneRIF: An Extended Iranian Family with Autosomal Dominant
            Non-syndromic Hearing Loss Associated with A Nonsense Mutation in
            the DIAPH1 Gene.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5735)
  AUTHORS   Kato,T., Watanabe,N., Morishima,Y., Fujita,A., Ishizaki,T. and
            Narumiya,S.
  TITLE     Localization of a mammalian homolog of diaphanous, mDia1, to the
            mitotic spindle in HeLa cells
  JOURNAL   J Cell Sci 114 (Pt 4), 775-784 (2001)
   PUBMED   11171383
REFERENCE   7  (bases 1 to 5735)
  AUTHORS   Lynch,E.D., Lee,M.K., Morrow,J.E., Welcsh,P.L., Leon,P.E. and
            King,M.C.
  TITLE     Nonsyndromic deafness DFNA1 associated with mutation of a human
            homolog of the Drosophila gene diaphanous
  JOURNAL   Science 278 (5341), 1315-1318 (1997)
   PUBMED   9360932
REFERENCE   8  (bases 1 to 5735)
  AUTHORS   Reinhard,M., Giehl,K., Abel,K., Haffner,C., Jarchau,T., Hoppe,V.,
            Jockusch,B.M. and Walter,U.
  TITLE     The proline-rich focal adhesion and microfilament protein VASP is a
            ligand for profilins
  JOURNAL   EMBO J 14 (8), 1583-1589 (1995)
   PUBMED   7737110
REFERENCE   9  (bases 1 to 5735)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
REFERENCE   10 (bases 1 to 5735)
  AUTHORS   Leon,P.E., Raventos,H., Lynch,E., Morrow,J. and King,M.C.
  TITLE     The gene for an inherited form of deafness maps to chromosome 5q31
  JOURNAL   Proc Natl Acad Sci U S A 89 (11), 5181-5184 (1992)
   PUBMED   1350680
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA593734.1, AB209482.1,
            AY363395.1 and AY007129.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005219.4.
            
            Summary: This gene is a homolog of the Drosophila diaphanous gene,
            and has been linked to autosomal dominant, fully penetrant,
            nonsyndromic sensorineural progressive low-frequency hearing loss.
            Actin polymerization involves proteins known to interact with
            diaphanous protein in Drosophila and mouse. It has therefore been
            speculated that this gene may have a role in the regulation of
            actin polymerization in hair cells of the inner ear. Alternatively
            spliced transcript variants encoding distinct isoforms have been
            found for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB209482.1, BC143413.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389054.8/ ENSP00000373706.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DA593734.1         56-59
            5-610               AB209482.1         1-606
            611-1539            AY363395.1         525-1453
            1540-5333           AB209482.1         1536-5329
            5334-5735           AY007129.1         1567-1968
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.3"
     gene            1..5735
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /note="diaphanous related formin 1"
                     /db_xref="GeneID:1729"
                     /db_xref="HGNC:HGNC:2876"
                     /db_xref="MIM:602121"
     exon            1..203
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..3905
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein diaphanous homolog 1; mammalian diaphanous related
                     formin 1"
                     /codon_start=1
                     /product="protein diaphanous homolog 1 isoform 1"
                     /protein_id="NP_005210.3"
                     /db_xref="CCDS:CCDS43374.1"
                     /db_xref="GeneID:1729"
                     /db_xref="HGNC:HGNC:2876"
                     /db_xref="MIM:602121"
                     /translation="MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRL
                     MADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLD
                     MNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMP
                     LLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEII
                     RCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMN
                     ERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHI
                     RSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNE
                     VFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADP
                     DFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESD
                     FEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLS
                     AAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGG
                     VCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLP
                     GSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPP
                     PPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFW
                     TKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKT
                     AQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEY
                     DDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSES
                     FSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCEND
                     YPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVE
                     KMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFL
                     QAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLE
                     ALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILE
                     EAKELVGRAS"
     exon            204..230
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            231..386
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            387..488
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            489..619
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            620..706
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            707..770
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            771..910
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1019
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1130
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1249
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1366
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1482
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1547
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1727
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..2444
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2559
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2568
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2667
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2762
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2763..2864
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..3104
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3105..3234
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3235..3359
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3360..3524
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3525..3660
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3661..3747
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3748..5735
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5716..5721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /note="hexamer: AATAAA"
     polyA_site      5735
                     /gene="DIAPH1"
                     /gene_synonym="DFNA1; DIA1; DRF1; hDIA1; LFHL1; mDia1;
                     SCBMS"
                     /note="major polyA site"
ORIGIN      
        1 taaagagcga ctgggcggcg gcgggcgcgg agctgccagg cgggagcggc gtaggcgcgg
       61 ggtcgccggc cagcgtgaac cgggacatgg agccgcccgg cgggagcctg gggcccggcc
      121 gcgggacccg ggacaagaag aagggccgga gcccagatga gctgccctcg gcgggcggcg
      181 acggcggcaa atctaagaaa tttactctga agcggctcat ggcagatgag ctggagagat
      241 ttaccagcat gagaattaag aaggagaagg aaaagcccaa ttctgctcat agaaattctt
      301 ctgcatcata tggggatgat cccacagcac agtcattgca agatgtttca gatgaacaag
      361 tgctggttct ctttgaacag atgctgctgg atatgaacct gaatgaggag aaacagcaac
      421 ctttgaggga gaaggacatc atcatcaaga gggagatggt gtcccaatac ttgtacacct
      481 ccaaggctgg catgagccag aaggagagct ctaagtctgc catgatgtat attcaggagt
      541 tgaggtcagg cttgcgggat atgcctctgc tcagctgcct ggagtccctt cgtgtgtctc
      601 tcaacaacaa ccctgtcagt tgggtgcaaa catttggtgc tgaaggcttg gcctccttat
      661 tggacattct taaacgactt catgatgaga aagaagagac tgctgggagt tacgatagcc
      721 ggaacaagca tgagatcatt cgctgcttga aagcttttat gaacaacaag tttggaatca
      781 agaccatgtt ggagacagaa gaaggaatcc tactgctggt cagagccatg gatcctgctg
      841 ttcccaacat gatgattgat gcagctaagc tgctttctgc tctttgtatt ctaccgcagc
      901 cagaggacat gaatgaaagg gttttggagg caatgacaga aagagctgag atggatgaag
      961 tggaacgttt ccagccgctg ctggatggat taaaaagtgg aaccactatt gcactgaagg
     1021 ttggatgcct acagctgatc aatgctctca tcacaccagc ggaggaactt gacttccgag
     1081 ttcacatcag aagtgaactg atgcgtttgg ggctacatca ggtgttgcag gaccttcgag
     1141 agattgaaaa tgaagatatg agagtgcaac taaatgtgtt tgatgaacaa ggggaagagg
     1201 attcctatga cctgaaggga cggctggatg acattcgcat ggagatggat gactttaatg
     1261 aagtctttca gattctctta aacacagtga aggattcaaa ggcagagcca cacttccttt
     1321 ccatcctgca gcacttactc ttggtccgaa atgactatga ggccagacct cagtactata
     1381 agttgattga agaatgtatt tcccagatag ttctgcacaa gaacggggct gatcctgact
     1441 tcaagtgccg gcacctccag attgagattg agggattaat tgatcaaatg attgataaga
     1501 caaaggtgga gaaatctgaa gccaaagctg cagagctgga aaagaagttg gactcagagt
     1561 taacagcccg acatgagcta caggtggaaa tgaaaaagat ggaaagtgac tttgagcaga
     1621 agcttcaaga tcttcaggga gaaaaagatg cactgcattc tgaaaagcag caaattgcca
     1681 cagagaaaca ggacctggaa gcagaggtgt cccagctcac aggagaggtt gccaagctga
     1741 caaaggaact ggaagatgcc aagaaagaaa tggcttccct ctctgcggca gctattactg
     1801 tacctccttc tgttcctagt cgtgctcctg ttccccctgc ccctccttta cctggtgact
     1861 ctggcactat tattccacca ccacctgctc ctggggatag taccactcct cctcctcctc
     1921 ctcctcctcc tcctcctcca cctcctttgc ctgggggtgt ttgcatctcc tcaccccctt
     1981 ctttacctgg aggtactgct atctctccac cccctccttt gtctggggat gctaccatcc
     2041 ctccaccccc tcctttgcct gagggtgttg gcatcccttc accctcttct ttgcctggag
     2101 gtactgccat ccccccacct cctcctttgc ctgggagtgc tagaatcccc ccaccaccac
     2161 ctcctttgcc tgggagtgct ggaattcccc ccccacctcc tcccttgcct ggagaagcag
     2221 gaatgccacc tcctcctccc cctcttcctg gtggtcctgg aatccctcca cctcctccat
     2281 ttcccggagg ccctggcatt cctccacctc cacccggaat gggtatgcct ccacctcccc
     2341 catttggatt tggagttcct gcagccccag ttctgccatt tggattaacc cccaaaaagc
     2401 tttataagcc agaggtgcag ctccggaggc caaactggtc caagcttgtg gctgaggacc
     2461 tctcccagga ctgcttctgg acaaaggtga aggaggaccg ctttgagaac aatgaacttt
     2521 tcgccaaact tacccttacc ttctctgccc agaccaagac ttccaaagcc aagaaggatc
     2581 aagaaggtgg agaagaaaag aaatctgtgc aaaagaaaaa agtaaaagag ttaaaggtgt
     2641 tggattcaaa gacagcccag aatctctcaa tctttttggg ttccttccgc atgccctatc
     2701 aagagattaa gaatgtcatc ctggaggtga atgaggctgt tctgactgag tctatgatcc
     2761 agaacctcat taagcaaatg ccagagccag agcagttaaa aatgctttct gaactgaagg
     2821 atgaatatga tgacctggct gagtcagagc agtttggcgt ggtgatgggc actgtgcccc
     2881 gactgcggcc tcgcctcaat gccattctct tcaagctaca attcagcgag caagtggaga
     2941 atatcaagcc agagattgtg tctgtcactg ctgcatgtga ggagttacgt aagagtgaga
     3001 gcttttccaa tctcctagag attaccttgc ttgttggaaa ttacatgaat gctggctcca
     3061 gaaatgctgg tgcttttggc ttcaatatca gcttcctctg taagcttcga gacaccaagt
     3121 ccacagatca gaagatgacg ttgttacact tcttggctga gttgtgtgag aatgactatc
     3181 ccgatgtcct caagtttcca gacgagcttg cccatgtgga gaaagccagc cgagtttctg
     3241 ctgaaaactt gcaaaagaac ctagatcaga tgaagaaaca aatttctgat gtggaacgtg
     3301 atgttcagaa tttcccagct gccacagatg aaaaagacaa gtttgttgaa aaaatgacca
     3361 gctttgtgaa ggatgcacag gaacagtata acaagctgcg gatgatgcat tctaacatgg
     3421 agaccctcta taaggagctg ggcgagtact tcctctttga ccccaagaag ttgtctgttg
     3481 aagaattttt catggatctt cacaattttc ggaatatgtt tttgcaagca gtcaaggaga
     3541 accagaagcg gcgggagaca gaagaaaaga tgaggcgagc aaaactagcc aaggagaagg
     3601 cagagaagga gcggctagag aagcagcaga agagagagca actcatagac atgaatgcag
     3661 agggcgatga gacaggtgtg atggacagtc ttctagaagc cctgcagtca ggggcagcat
     3721 tccgacggaa gagagggccc cgtcaagcca acaggaaggc cgggtgtgca gtcacatctc
     3781 tgctagcttc ggagctgacc aaggatgatg ccatggctgc tgttcctgcc aaggtgtcca
     3841 agaacagtga gacattcccc acaatccttg aggaagccaa ggagttggtt ggccgtgcaa
     3901 gctaatgtgg gtcctgtgac cgcggcagct cctcagcgga gccgcagact gtcctgccct
     3961 gcagcatgtg cctaaaggct caaggggata ttcctctggg gtggccactc ccaccaccct
     4021 gaccctgtct ttctctctgg cctgctgctc tctcaacatc acatacagct tcagctgcct
     4081 ggaggccaga aggaaagggc agtgcagggg aggcctgagc ccgacttagc cagccctggc
     4141 tgttgtatta ccaaagcagg gtccatgttt gctgccttaa ccctgtctcc tctctgttac
     4201 tcagagggcc tcatctcaga caaggcccag cctgcttttt ctcagccctg actttctaat
     4261 gggctttccc ccctaggtca gtcttgctgg atttgtgctt ttcttttgtg gtttctctgg
     4321 ccctgagaat agcatggggc ttgtaaacct ttgggctaga tccctccttt cattgctgtt
     4381 gtctctgctc ttccctctcc tggctgtggt tatttattat tagtggtgtg gcactgggag
     4441 ctgctcctaa ggaagcaggg agcaaatccc acctttaccc caccttcctg ggaaaggcct
     4501 ccaaagcaaa ggatctggac cagtttccct gctgtgctgt ggcccaggcc agagcctgtg
     4561 ggcaggcagg cagggcatag cgacagtgtg ggacctgccc ccagcttctg ccacgcttta
     4621 tgcccttgcc tctctggacg ctctgcacca accccaggct actgagccac cttccctcct
     4681 catgccttcc ctgagctttg gtgcatctca tctggactat gggttgtact gtgaccatcc
     4741 caacacctca ccctctgtct acaaggaaat gggaggtgga gcctcctggc tgagaaattg
     4801 ttttgcaaat ggatctattt ttgtatgaaa aaaaaaattt ttttaaagaa aactgttcct
     4861 tccccctttc ccctccataa tgtaagaagc tttggtggca ggttacagag ttctgggatt
     4921 tcttctcaca ggcccaatcc tgaatgtgcc cctggacctt ctggaccctt gagtccaagg
     4981 cagatcctct ctcccaggga atccgacaca ggaggaaccc cttctctggt tgagctgggc
     5041 caggcctaag agtagcagga actctaagac cacagagttt tttataaatg tataaatgta
     5101 tcaagccaaa tgtgcagatg ctaactggac attctgggga actgggcacc aggagtgcct
     5161 tcatacactg taccccagct ctcttctaaa agagaagtgg gtgggcacac tgaactgttt
     5221 ggtggcccca accacaggaa gctgcaattc tctggcttag ggtgatactt ttgccctcct
     5281 tgtgcccctc tcagctttcc atccccagct aggaagaaag aatggcactc ttgggcttgg
     5341 cccagaatta gagttattag agcaagagag agcttaggaa gcatgagggc aactatagtg
     5401 aggccttatt gccaggaggg agggttttgg ttgctggcgc ttgtgtataa aggggcaaga
     5461 gcagctcctt tggactattc ctgggaggac tctgatgcag ggcgtctgtt gctcccctgg
     5521 gtcacctcct ccctgctcgc tgacatctgg ggctttgacc ctttcttttt taatctactt
     5581 ttgctaagat gcatttaata aaaaaaaaga gagagagaga gaggtgtgag ggacaaaatg
     5641 caaacctatt tcccttgcct cataggcttc tgggatgtca tcacctccag tttgttggtt
     5701 ttgtttccaa ctgttaataa agcattgaaa cagta
//
