LOCUS       NM_004941               5549 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1,
            mRNA.
ACCESSION   NM_004941
VERSION     NM_004941.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Wei,G., Chen,X., Ruan,T., Ma,X., Zhu,X., Wen,W., He,D., Tao,K. and
            Wu,C.
  TITLE     Human gastric cancer progression and stabilization of ATG2B through
            RNF5 binding facilitated by autophagy-associated CircDHX8
  JOURNAL   Cell Death Dis 15 (6), 410 (2024)
   PUBMED   38866787
  REMARK    GeneRIF: Human gastric cancer progression and stabilization of
            ATG2B through RNF5 binding facilitated by autophagy-associated
            CircDHX8.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Osterhoudt,K., Bagno,O., Katzman,S. and Zahler,A.M.
  TITLE     Spliceosomal helicases DDX41/SACY-1 and PRP22/MOG-5 both contribute
            to proofreading against proximal 3' splice site usage
  JOURNAL   RNA 30 (4), 404-417 (2024)
   PUBMED   38282418
  REMARK    GeneRIF: Spliceosomal helicases DDX41/SACY-1 and PRP22/MOG-5 both
            contribute to proofreading against proximal 3' splice site usage.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5549)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5549)
  AUTHORS   Felisberto-Rodrigues,C., Thomas,J.C., McAndrew,C., Le Bihan,Y.V.,
            Burke,R., Workman,P. and van Montfort,R.L.M.
  TITLE     Structural and functional characterisation of human RNA helicase
            DHX8 provides insights into the mechanism of RNA-stimulated ADP
            release
  JOURNAL   Biochem J 476 (18), 2521-2543 (2019)
   PUBMED   31409651
  REMARK    GeneRIF: This study provides an in-depth understanding of the
            activity of DHX8 and contributes insights into the RNA-unwinding
            mechanisms of the DEAH-box helicase family.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5549)
  AUTHORS   Neuhaus,E.M., Mashukova,A., Barbour,J., Wolters,D. and Hatt,H.
  TITLE     Novel function of beta-arrestin2 in the nucleus of mature
            spermatozoa
  JOURNAL   J Cell Sci 119 (Pt 15), 3047-3056 (2006)
   PUBMED   16820410
REFERENCE   6  (bases 1 to 5549)
  AUTHORS   Tao,W.A., Wollscheid,B., O'Brien,R., Eng,J.K., Li,X.J.,
            Bodenmiller,B., Watts,J.D., Hood,L. and Aebersold,R.
  TITLE     Quantitative phosphoproteome analysis using a dendrimer conjugation
            chemistry and tandem mass spectrometry
  JOURNAL   Nat Methods 2 (8), 591-598 (2005)
   PUBMED   16094384
REFERENCE   7  (bases 1 to 5549)
  AUTHORS   Andersen,J.S., Lam,Y.W., Leung,A.K., Ong,S.E., Lyon,C.E.,
            Lamond,A.I. and Mann,M.
  TITLE     Nucleolar proteome dynamics
  JOURNAL   Nature 433 (7021), 77-83 (2005)
   PUBMED   15635413
REFERENCE   8  (bases 1 to 5549)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   9  (bases 1 to 5549)
  AUTHORS   Ohno,M. and Shimura,Y.
  TITLE     A human RNA helicase-like protein, HRH1, facilitates nuclear export
            of spliced mRNA by releasing the RNA from the spliceosome
  JOURNAL   Genes Dev 10 (8), 997-1007 (1996)
   PUBMED   8608946
REFERENCE   10 (bases 1 to 5549)
  AUTHORS   Ono,Y., Ohno,M. and Shimura,Y.
  TITLE     Identification of a putative RNA helicase (HRH1), a human homolog
            of yeast Prp22
  JOURNAL   Mol Cell Biol 14 (11), 7611-7620 (1994)
   PUBMED   7935475
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D50487.1, BC044586.1 and
            AC068675.17.
            
            On May 17, 2019 this sequence version replaced NM_004941.2.
            
            Summary: This gene is a member of the DEAH box polypeptide family.
            The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which
            is characteristic of all DEAH box proteins, and is thought to
            function as an ATP-dependent RNA helicase that regulates the
            release of spliced mRNAs from spliceosomes prior to their export
            from the nucleus. This protein may be required for the replication
            of human immunodeficiency virus type 1 (HIV-1). Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Oct 2014].
            
            Transcript Variant:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.27203.1,
                                           SRR1660807.238146.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262415.8/ ENSP00000262415.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4191              D50487.1           11-4201
            4192-4200           BC044586.1         4147-4155
            4201-5549           AC068675.17        133012-134360       c
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.31"
     gene            1..5549
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /note="DEAH-box helicase 8"
                     /db_xref="GeneID:1659"
                     /db_xref="HGNC:HGNC:2749"
                     /db_xref="MIM:600396"
     exon            1..211
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..3726
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA
                     helicase); ATP-dependent RNA helicase DHX8; RNA helicase
                     HRH1; DEAH box protein 8; DEAH (Asp-Glu-Ala-His) box
                     polypeptide 8"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DHX8 isoform 1"
                     /protein_id="NP_004932.1"
                     /db_xref="CCDS:CCDS11464.1"
                     /db_xref="GeneID:1659"
                     /db_xref="HGNC:HGNC:2749"
                     /db_xref="MIM:600396"
                     /translation="MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGI
                     NDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSK
                     DPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQE
                     KQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKV
                     KSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIM
                     QFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLS
                     MKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLT
                     RISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLR
                     GHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKH
                     WVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLP
                     IYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAM
                     SVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAII
                     MLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGR
                     TYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMK
                     SLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVD
                     PGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLT
                     TNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGL
                     LTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQ
                     KKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDR
                     HKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQP
                     EWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNR
                     YEEPNAWRISRAFRRR"
     exon            212..297
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            298..370
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            371..456
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            457..566
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            567..926
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1071
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1275
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1363
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1461
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1609
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1791
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1986
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2172
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2383
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2565
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2706
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2862
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..3000
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3129
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3326
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3506
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     exon            3507..5549
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4200
                     /gene="DHX8"
                     /gene_synonym="DDX8; Dhr2; HRH1; PRP22; PRPF22"
                     /note="major polyA site"
ORIGIN      
        1 aacccggccg gagtagctct gagcgccggc tgtgaggaag gaggttctgg gcaagctata
       61 gccatggctg tggctgtagc catggcggga gccttaatcg ggtcggagcc aggccccgcg
      121 gaagaacttg ccaaactcga gtacctgtct ttggtgtcaa aggtttgcac tgagctggac
      181 aatcacttgg ggatcaacga caaggacctt gctgaatttg tgatcagtct tgctgagaaa
      241 aataccacct ttgatacttt taaggcttct ctcgtcaaaa atggtgcaga atttacggat
      301 tctcttatta gtaacttgct gcgtctcata caaaccatgc ggcctccagc gaagccttcc
      361 actagcaaag atccagttgt taaacctaaa acagaaaaag aaaagctgaa ggaactcttc
      421 ccagtccttt gccaaccgga caacccttct gttcggacca tgttggatga agatgatgtg
      481 aaagttgctg tggatgtcct gaaagaactg gaagctttaa tgcccagcgc agcaggccag
      541 gagaagcaaa gagatgctga acaccgggac aggacaaaga agaagaagcg gagtcgaagc
      601 cgagatcgaa accgagatcg agacagagat agggaacgaa accgagatag agaccacaag
      661 cggagacacc gatcccgctc tcgatcacgt tccaggaccc gggagaggaa taaagtgaag
      721 tctagatatc ggtccaggag caggagtcag agtcccccca aagaccggaa ggaccgggac
      781 aaatatggag agcggaatct ggatagatgg cgggataagc atgtggaccg ccctcctcca
      841 gaagagccca ccattggtga catttataat ggcaaagtta ccagcatcat gcagtttggt
      901 tgctttgtgc agctggaagg actaaggaag cggtgggaag gcctggtgca catctctgag
      961 ctccggcggg agggtcgtgt ggccaatgta gctgatgtcg tgagcaaagg ccagagggtc
     1021 aaagtcaaag tgctgtcctt cactgggacc aagaccagcc tgagcatgaa ggatgtggat
     1081 caagagactg gagaagatct aaacccaaat agacggcgaa atcttgtcgg ggagaccaat
     1141 gaggagacct caatgcggaa tcctgataga cccactcact tgtcccttgt cagtgctcct
     1201 gaagtagagg acgactcact ggaacgcaag cgcctcaccc gaatctctga cccagagaag
     1261 tgggagatca aacagatgat tgctgccaat gtcctttcca aagaagaatt tccagacttt
     1321 gatgaagaga ctggcattct ccctaaggtg gatgatgaag aagatgagga ccttgagatt
     1381 gaattggttg aggaagagcc tccattcctg agagggcaca ctaagcaaag catggacatg
     1441 agccccatta aaattgtcaa gaacccagac ggctccctct cccaagcagc aatgatgcag
     1501 agtgccttgg ccaaagaaag gcgggaactc aaacaggccc agcgggaagc tgagatggat
     1561 tctattccca tgggactcaa caaacactgg gttgaccctc tgcctgatgc ggaaggcaga
     1621 cagattgctg ccaacatgag gggtattggg atgatgccca atgatattcc tgagtggaag
     1681 aagcatgcct ttgggggcaa caaagcctct tacggaaaaa agacccagat gtcaatcctt
     1741 gagcagaggg agagcctgcc catctacaaa ctgaaggagc aattggtcca ggccgtccat
     1801 gacaatcaga tcctgattgt cattggtgag acaggatctg gaaagacaac acagatcacc
     1861 cagtacctgg cggaggcagg ctacacttcc aggggcaaga ttgggtgtac ccagcccaga
     1921 agagtggcag ctatgtcggt ggccaaaaga gtgtcagagg agtttggttg ttgcttaggc
     1981 caagaggtgg gctacaccat tcgatttgag gactgcacta gccctgaaac agtcatcaag
     2041 tacatgacag atgggatgtt gcttagagag tgcttgattg accctgacct cactcagtac
     2101 gcgatcatca tgttggacga ggcacatgag aggacaattc acactgatgt gctctttgga
     2161 ttgttgaaaa agacagttca gaaacggcag gacatgaagc tgattgtcac ctcagccacc
     2221 ttggatgcag tgaagttttc tcaatacttc tatgaagctc ccattttcac catcccaggt
     2281 cgaacatatc cagtggaaat actgtacaca aaggaacctg agacagatta tctggatgcc
     2341 agcctgatta ctgttatgca gattcattta acagaaccac caggtgatat cctggtcttc
     2401 ctgactggtc aggaagaaat tgatactgct tgtgagatcc tgtatgaaag aatgaaatcc
     2461 ctgggacctg atgttccaga gttaattatc ctcccagtgt actctgctct tcccagtgag
     2521 atgcagaccc gaatctttga cccagctcca ccaggcagca gaaaggttgt gattgccacc
     2581 aatatcgcag agacatcgct gactattgat ggtatctact atgtggtgga cccaggattc
     2641 gtgaaacaga aagtttacaa ttccaagaca gggattgacc agctcgtggt gacgcctatt
     2701 tctcaggctc aggcaaagca acgagctggc agagctggga gaacaggccc agggaagtgt
     2761 tacaggttgt acacagaacg tgcctaccga gatgaaatgc tgaccaccaa cgtgccggaa
     2821 atccagagaa ccaacttagc aagcacagtg ctgtcactca aggccatggg tatcaatgat
     2881 ctgctgtcct ttgatttcat ggatgcccca cctatggaaa ctttgatcac agccatggag
     2941 cagctgtaca cactgggggc cctggatgac gagggcctgc tcactcgctt gggccgccgg
     3001 atggcagagt tccctctgga gccaatgcta tgcaaaatgc tcatcatgtc tgtgcatctg
     3061 ggctgcagtg aggaaatgct gaccattgta tccatgctgt ctgtgcagaa cgtcttctat
     3121 aggcccaagg ataaacaagc ccttgcagat cagaagaagg ccaaattcca ccagactgaa
     3181 ggggaccacc tcaccctgct agctgtgtac aactcctgga agaacaacaa gttctccaac
     3241 ccatggtgct atgagaactt tatccaggct cgttccctgc gccgggccca ggacattcgc
     3301 aagcagatgt taggcataat ggacagacac aagctggatg ttgtttcctg tggcaagtcc
     3361 acagtccgag tgcagaaggc catctgcagt gggttcttcc gtaatgctgc caagaaagac
     3421 ccgcaggagg gttaccggac actgatcgac cagcaggtgg tctatatcca tccttccagt
     3481 gccctcttca acagacagcc agaatgggtg gtgtaccatg agctggtgct caccaccaag
     3541 gaatacatgc gtgaagttac caccatcgac cctcggtggc ttgtggagtt tgccccagcc
     3601 ttcttcaagg tctcagaccc aactaagcta agcaaacaga agaagcaaca gcgtcttgaa
     3661 cccttgtaca accgctatga ggaacccaat gcctggagaa tatctcgagc tttccgacgg
     3721 cgctgaaagg caagattgtt cctttgcctc tccagcagca gtagccaggg cttggactta
     3781 tcgatgacag gctggtcctg aggatacagc tgtcccgtga ctgactgtct taactgagca
     3841 ttttctcaac tcgactctca tttcttccct gctggtaaaa tagaaacagg gatttaaacc
     3901 tggctttggc aagagcctgc agcctccatc accccaagtc cttgggccca gttgggagct
     3961 catatctaac acagagacac attgcatcaa cttcaagaaa gggacaattt gtgcagctcc
     4021 aggatgggaa ggtggagtgg gtgagcatct tgtgcaggga catggtgagt gccctgatgc
     4081 cccagctagc aggagctact gtgctcatct aaagtgtttg ccccacttcc caccccgtct
     4141 ccagcccctg tactttggct tgacctcgtg gaaatattta ttttcttaag gaaacaaaaa
     4201 tggttttctg tgactgtttt cttttagccg aaaggttagg atattggcct gggctcaggg
     4261 tgagggagat ttagtatctg tgctctggca cacatgatga gaatcccctg aatcccctga
     4321 aaccagaacg cagggcctct ttgcgctcgg aaacgacgta caacccagac ttccagcctt
     4381 gtctttcagc atcagcacta gccgggccgt gctgggggat cacccgatga tcaaccatgt
     4441 cctctccaca gagcacttca gtctggaggc ctgagtggct acagatggca catattaaat
     4501 acagaaggtt tccccttgct ccctccacct cccacattcg caatgtgcag catgtcccat
     4561 ttccttttga aacatactaa gtaacaacac agcttttatt ttattttgtt tttatttttt
     4621 tcccctgagg tcctggatgg actttaacaa agggatttta tggtcaaaac ctccctttcc
     4681 ccactccaaa cagaaaacaa acataacacc tctcctctca gctggtttgt gctgccagta
     4741 tctgtgctgc agggcttgtt cttagtggtt ttttcctagc acttgcttgg agaatagcca
     4801 cctccttgtg ccctcctcac agctcctgag gagggttttt tttttttctt cccatagaga
     4861 tgggttccca ctgtgctgcc caggctgctc tccaatccct ggcgtcaagc aatcctcctg
     4921 cctctgcctc ccaaagcgct gggattacag ttgagagcca ctgtcctctg cactgaggag
     4981 gctccttacc tggcggaaca tcaggcaggt cttgccaggc caggtttcgg aacttacgga
     5041 aagctggtct ggatgtagtg cttgtagaga agcttctgag agattgggca catcctgtta
     5101 cgttgctgct tctcctgtcc ttatgttatt agtaagttct gaagtgatgt aaatgctaga
     5161 actactgtag aactgtatgc cagacactaa gagagactat gtaacattcc aggatataaa
     5221 ggaatgtatg ttgtcactgg gcagcaaacc acaccctaaa gacaattcag aaagagtccg
     5281 agggagagga atataggcca ggcaatctgc tggctgcaga tccctgcccc ttcattaagc
     5341 tgagtgcctc ctgattctgt ctgagtattc gcctttgtaa gcccaggcca gtaatcaagg
     5401 ggcagggcat tgttgtgtgt taaccttgaa ggccttctgg ggagagacag tacagggacc
     5461 tcaaatgaaa ccaaagggga aacggggact gggcacccac ctccccaatc tcgttttgtt
     5521 tttgtttttg ttaagacagg atcttgcca
//
