LOCUS       NM_020865               6723 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens DEAH-box helicase 36 (DHX36), transcript variant 1,
            mRNA.
ACCESSION   NM_020865
VERSION     NM_020865.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Gumina,J.M., Richardson,A.E., Shojiv,M.H., Chambers,A.E.,
            Sandwith,S.N., Reisinger,M.A., Karns,T.J., Osborne,T.L.,
            Alashi,H.N., Anderson,Q.T., Sharlow,M.E., Seiler,D.C., Rogers,E.M.,
            Bartosik,A.R., Smaldino,M.A., Vaughn,J.P., Wang,Y.H., Smaldino,P.J.
            and Haney,R.A.
  TITLE     Differential Gene Expression following DHX36/G4R1 Knockout Is
            Associated with G-Quadruplex Content and Cancer
  JOURNAL   Int J Mol Sci 25 (3), 1753 (2024)
   PUBMED   38339029
  REMARK    GeneRIF: Differential Gene Expression following DHX36/G4R1 Knockout
            Is Associated with G-Quadruplex Content and Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Mizumoto,A., Yokoyama,Y., Miyoshi,T., Takikawa,M., Ishikawa,F. and
            Sadaie,M.
  TITLE     DHX36 maintains genomic integrity by unwinding G-quadruplexes
  JOURNAL   Genes Cells 28 (10), 694-708 (2023)
   PUBMED   37632696
  REMARK    GeneRIF: DHX36 maintains genomic integrity by unwinding
            G-quadruplexes.
REFERENCE   3  (bases 1 to 6723)
  AUTHORS   Antcliff,A., McCullough,L.D. and Tsvetkov,A.S.
  TITLE     G-Quadruplexes and the DNA/RNA helicase DHX36 in health, disease,
            and aging
  JOURNAL   Aging (Albany NY) 13 (23), 25578-25587 (2021)
   PUBMED   34862880
  REMARK    GeneRIF: G-Quadruplexes and the DNA/RNA helicase DHX36 in health,
            disease, and aging.
            Review article
REFERENCE   4  (bases 1 to 6723)
  AUTHORS   Patil,K.M., Chin,D., Seah,H.L., Shi,Q., Lim,K.W. and Phan,A.T.
  TITLE     G4-PROTAC: targeted degradation of a G-quadruplex binding protein
  JOURNAL   Chem Commun (Camb) 57 (95), 12816-12819 (2021)
   PUBMED   34783801
  REMARK    GeneRIF: G4-PROTAC: targeted degradation of a G-quadruplex binding
            protein.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6723)
  AUTHORS   Tseng,Y.J., Sandwith,S.N., Green,K.M., Chambers,A.E., Krans,A.,
            Raimer,H.M., Sharlow,M.E., Reisinger,M.A., Richardson,A.E.,
            Routh,E.D., Smaldino,M.A., Wang,Y.H., Vaughn,J.P., Todd,P.K. and
            Smaldino,P.J.
  TITLE     The RNA helicase DHX36-G4R1 modulates C9orf72 GGGGCC hexanucleotide
            repeat-associated translation
  JOURNAL   J Biol Chem 297 (2), 100914 (2021)
   PUBMED   34174288
  REMARK    GeneRIF: The RNA helicase DHX36-G4R1 modulates C9orf72 GGGGCC
            hexanucleotide repeat-associated translation.
REFERENCE   6  (bases 1 to 6723)
  AUTHORS   Vaughn,J.P., Creacy,S.D., Routh,E.D., Joyner-Butt,C., Jenkins,G.S.,
            Pauli,S., Nagamine,Y. and Akman,S.A.
  TITLE     The DEXH protein product of the DHX36 gene is the major source of
            tetramolecular quadruplex G4-DNA resolving activity in HeLa cell
            lysates
  JOURNAL   J Biol Chem 280 (46), 38117-38120 (2005)
   PUBMED   16150737
  REMARK    GeneRIF: DHX36 protein is responsible for the majority of
            tetramolecular G4-DNA resolvase activity
REFERENCE   7  (bases 1 to 6723)
  AUTHORS   Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
            Stettler-Gill,M. and Peters,E.C.
  TITLE     Robust phosphoproteomic profiling of tyrosine phosphorylation sites
            from human T cells using immobilized metal affinity chromatography
            and tandem mass spectrometry
  JOURNAL   Anal Chem 76 (10), 2763-2772 (2004)
   PUBMED   15144186
REFERENCE   8  (bases 1 to 6723)
  AUTHORS   Tran,H., Schilling,M., Wirbelauer,C., Hess,D. and Nagamine,Y.
  TITLE     Facilitation of mRNA deadenylation and decay by the exosome-bound,
            DExH protein RHAU
  JOURNAL   Mol Cell 13 (1), 101-111 (2004)
   PUBMED   14731398
REFERENCE   9  (bases 1 to 6723)
  AUTHORS   Fu,J.J., Li,L.Y., Liu,S.F., Xing,X.W., Liu,G. and Lu,G.X.
  TITLE     Expression research for human DDX36 and mouse Ddx36 gene in the
            adult testis
  JOURNAL   Yi Chuan Xue Bao 30 (3), 201-208 (2003)
   PUBMED   12812084
REFERENCE   10 (bases 1 to 6723)
  AUTHORS   Fu,J.J., Li,L.Y. and Lu,G.X.
  TITLE     Molecular cloning and characterization of human DDX36 and mouse
            Ddx36 genes, new members of the DEAD/H box superfamily
  JOURNAL   Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34 (5),
            655-661 (2002)
   PUBMED   12198572
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK314435.1, BC036035.1 and AC018452.11.
            
            On Nov 22, 2018 this sequence version replaced NM_020865.2.
            
            Summary: This gene is a member of the DEAH-box family of
            RNA-dependent NTPases which are named after the conserved amino
            acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by
            this gene has been shown to enhance the deadenylation and decay of
            mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also
            been shown to resolve into single strands the highly stable
            tetramolecular DNA configuration (G4) that can form spontaneously
            in guanine-rich regions of DNA. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1). Isoform 1 localizes
            to the nucleus and cytoplasm.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF217190.1, SRR1660805.58346.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000496811.6/ ENSP00000417078.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3098              AK314435.1         11-3108
            3099-3601           BC036035.1         2986-3488
            3602-6723           AC018452.11        6376-9497
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.2"
     gene            1..6723
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /note="DEAH-box helicase 36"
                     /db_xref="GeneID:170506"
                     /db_xref="HGNC:HGNC:14410"
                     /db_xref="MIM:612767"
     exon            1..314
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..3098
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /EC_number="3.6.4.13"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36; DEAH
                     (Asp-Glu-Ala-His) box polypeptide 36; probable
                     ATP-dependent RNA helicase DHX36; G4 resolvase-1; MLE-like
                     protein 1; DEAH box protein 36; RNA helicase associated
                     with AU-rich element ARE; DEAD/H box polypeptide 36; RNA
                     helicase associated with AU-rich element protein;
                     ATP-dependent DNA/RNA helicase DHX36"
                     /codon_start=1
                     /product="ATP-dependent DNA/RNA helicase DHX36 isoform 1"
                     /protein_id="NP_065916.2"
                     /db_xref="CCDS:CCDS3171.1"
                     /db_xref="GeneID:170506"
                     /db_xref="HGNC:HGNC:14410"
                     /db_xref="MIM:612767"
                     /translation="MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGG
                     GGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSV
                     QAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNR
                     SYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVN
                     LIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERV
                     AAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLD
                     EIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFP
                     VVEYLLEDVIEKIRYVPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPDYVREL
                     RRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLL
                     MSQVMFKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYV
                     IDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLD
                     DYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDK
                     QEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKI
                     ADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMK
                     GQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKM
                     VKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFG
                     GDISIQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDT
                     KSRDCAVLSAIIDLIKTQEKATPRNFPPRFQDGYYS"
     exon            315..439
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            440..674
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            675..713
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            714..884
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            885..964
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1039
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1206
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1288
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1429
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1532
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1620
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1676
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1741
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1885
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2003
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2102
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2276
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2363
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2447
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2547
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2638
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2639..2759
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2912
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..6723
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3576..3581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /note="hexamer: AATAAA"
     polyA_site      3601
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /note="major polyA site"
     regulatory      4383..4388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /note="hexamer: GATAAA"
     polyA_site      4411
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
     regulatory      6704..6709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
                     /note="hexamer: AATAAA"
     polyA_site      6723
                     /gene="DHX36"
                     /gene_synonym="DDX36; G4R1; MLEL1; RHAU"
ORIGIN      
        1 acagtggctt ccgggcccgg acgccatttc cagcggttgc tggttctgac gggttgtagt
       61 ctgccaggac aatgagttat gactaccatc agaactgggg ccgtgatggg ggtccccgca
      121 gctccggtgg gggctatgga ggggggccag cagggggtca tggaggtaac cgaggctccg
      181 gaggaggcgg cggcggcgga gggggtggtc gaggcggcag gggccggcat cccgggcacc
      241 tgaaaggccg cgaaatcggc atgtggtacg cgaaaaaaca ggggcagaag aacaaggaag
      301 cggagaggca agagagagct gtagtacaca tggatgaacg acgagaagaa caaattgtac
      361 agttactgaa ttctgttcaa gcgaagaatg ataaagagtc agaagcacag atatcctggt
      421 ttgctcctga ggatcatgga tacggtactg aagtttctac taagaacaca ccatgctcag
      481 agaacaaact tgacatccag gaaaagaagt tgataaatca agaaaaaaaa atgtttagaa
      541 tcaggaacag atcatatatt gaccgagatt ctgagtatct cttgcaagaa aatgaaccag
      601 atggaacttt agaccaaaaa ttattggaag atttacaaaa gaaaaaaaat gaccttcggt
      661 atattgaaat gcagcatttc agagaaaagc tgccttcgta tggaatgcaa aaggaattgg
      721 taaatttaat tgataaccat caggtaacag taataagtgg tgaaactggt tgtggcaaaa
      781 ccactcaagt tactcagttc attttggata actacattga aagaggaaaa ggatctgctt
      841 gcagaatagt ttgtactcag ccaagaagaa ttagtgccat ttcagttgcg gaaagagtag
      901 ctgcagaaag ggcagaatct tgtggcagtg gtaatagtac tggatatcaa attcgtctcc
      961 agagtcggtt gccaaggaaa cagggttcta tcttatactg tacaacagga atcatccttc
     1021 agtggctcca gtcagacccg tatttgtcca gtgttagtca tatcgtactt gatgaaatcc
     1081 atgaaagaaa tctgcagtca gatgttttaa tgactgttgt taaagacctt ctcaattttc
     1141 gatctgactt gaaagtaata ttgatgagtg caacattgaa tgcagaaaag ttttcagaat
     1201 attttggtaa ctgtccaatg atacatatac ctggttttac ctttccggtt gtggaatatc
     1261 ttttggaaga tgtaattgaa aaaataaggt atgttccaga acaaaaagaa cacagatccc
     1321 agtttaagag gggtttcatg caagggcatg taaatagaca agaaaaagaa gaaaaagaag
     1381 caatatataa agaacgttgg ccagattatg taagggaact gcgaagaagg tattctgcaa
     1441 gtactgtaga tgttatagaa atgatggagg atgataaagt tgatctgaat ttgattgttg
     1501 ccctcatccg atacattgtt ttggaagaag aggatggtgc gatactggtc tttctgccag
     1561 gctgggacaa tatcagcact ttacatgatc tcttgatgtc acaagtaatg tttaaatcag
     1621 ataaattttt aattatacct ttacattcac tgatgcctac agttaaccag acacaggtgt
     1681 ttaaaagaac ccctcctggt gttcggaaaa tagtaattgc taccaacatt gcggagacta
     1741 gcattaccat agatgatgtc gtttatgtga tagatggagg aaaaataaaa gagacgcatt
     1801 ttgatactca gaacaatatc agtacaatgt ccgctgagtg ggttagtaaa gctaatgcca
     1861 aacagagaaa aggtcgagct ggaagagttc aacctggtca ttgctatcat ctgtataatg
     1921 gtcttagagc aagtcttcta gatgactatc aactgccaga aattttgaga actcctttgg
     1981 aagaactttg tttacaaata aagattttaa ggctaggtgg aattgcttat tttctgagta
     2041 gattaatgga cccaccatca aatgaggcag tgttactctc cataagacac ctgatggagc
     2101 tgaacgcttt ggataaacaa gaagaattga cacctcttgg agtccacttg gcacgattac
     2161 ccgttgagcc acatattgga aaaatgattc tttttggagc actgttctgc tgcttagacc
     2221 cagtactcac tattgctgct agtctcagtt tcaaagatcc atttgtcatt ccactgggaa
     2281 aagaaaagat tgcagatgca agaagaaagg aattggcaaa ggatactaga agtgatcact
     2341 taacagttgt gaatgcgttt gagggctggg aagaggctag gcgacgtggt ttcagatacg
     2401 aaaaggacta ttgctgggaa tattttctgt cttcaaacac actgcagatg ctgcataaca
     2461 tgaaaggaca gtttgctgag catcttcttg gagctggatt tgtaagcagt agaaatccta
     2521 aagatccaga atctaatata aattcagata atgagaagat aattaaagct gtcatctgtg
     2581 ctggtttata tcccaaagtt gctaaaattc gactaaattt gggtaaaaaa agaaaaatgg
     2641 taaaagttta cacaaaaacc gatggcctgg ttgctgttca tcctaaatct gttaatgtgg
     2701 agcaaacaga ctttcactac aactggctta tctatcacct aaagatgaga acaagcagta
     2761 tatacttgta tgactgcaca gaggtttccc catactgtct cttgtttttt ggaggtgaca
     2821 tttccatcca gaaggataac gatcaggaaa ctattgctgt agatgagtgg attgtatttc
     2881 agtctccagc aagaattgcc catcttgtta aggaattaag aaaggaacta gatattcttc
     2941 tgcaagagaa gattgaaagt cctcatcctg tagactggaa tgacactaaa tccagagact
     3001 gtgcagtact gtcagctatt atagacttga tcaaaacaca ggaaaaggca actcccagga
     3061 actttccgcc acgattccag gatggatatt acagctgaca gcttttcagg ggtggtctga
     3121 aaagccagtt tgacagccat tcttcatcat tgtttaaatt ttggctggat gccaaaccct
     3181 gggacatgaa caattttcat gtgtaaggta gaagccttca gtaggtagta aagacttaat
     3241 gtgcatgact tgatgttata tgtagagata tatatatata tatatatata ccataaaagc
     3301 aatatgttct ctgatcatat actctgctgt ggtcatgccc actctttggg agtatattcc
     3361 ctttatatat attgagtatt gtaccacttg agaaattcct ttgttctgtt atacaaaatt
     3421 aatctttctg ctcataatga ttgatgatac caccagtaaa aataggatgt ttaccccaaa
     3481 acaagtgtca attaagaatt tgaacacaac cacatttttt aaaatgaaac ttctatcgga
     3541 agtaaattaa tttgttgtaa taaagtccag tatttaataa aatgtacaat gttaaatctc
     3601 agccaacctc ttggattggt ttctacattt tcacctatgg cttggttcat ttgatatttt
     3661 tactattttt aaaacacgtg ccctttttat atcaccctgt gcctcataat acagcaattg
     3721 agagcacggt ctggagcctg attacctcag ttccagtctc agctctgtca gttagtcagt
     3781 gtcttggctt ctctcctttt tccttatctt gtaagataca gttggccctt catatccatg
     3841 cattccacat ctgtggattc aaacaactgt ggatagaaga tatttgggga aaaaaaggat
     3901 ggttgtgtct atactgaaca tgtgcagact tttttccttg tcattcccta aacaatacag
     3961 tttaacagct atttatatag tatttacatt gtattaggta ttataaataa tctagagatg
     4021 acttaaagaa tatgggaggt tgtgcctagg taacatgcaa atactacgcc attttattat
     4081 aaaagacttg agcatccatg gatttaggta tttgaggggt tggggttgga aaccagtccc
     4141 ccacagatac tgagggacaa ctgtacatgt aacagttgtg aggatgaagt acattaatac
     4201 atgtaaagca cataggctag tgcctgtact ctctgaatct tagttgttat ttgtccctga
     4261 aatattttcg taaatgtcta acaatctatt atattacact ttttatttta ttgctactta
     4321 tcttcttcgc caaggaatag tgtaacaaca catgctgtac attttcagcc ctatcagatt
     4381 gtgataaata ctgttggttg ccggccagca actattttgt ccctcccttc ttgcttgctt
     4441 attctggtgt tcccttctgt gcagctctgt gtccaggtat gttggaccct ttccctaagg
     4501 gtgaatcctg ctgttctaaa tgaatcacat gtggtcttat cccccttata agtaattgat
     4561 aaggcatgag tacataaaca tgtcctagtg aatggcagct gagaagtgat cttcaagggg
     4621 gtgtctgaaa aatgttttac tcactaatag gcttagagga ggaaatcctg ctctctctgg
     4681 acattgtctt ttctgactct gatagttgga actgctgcag ccaactaatg atcacaagga
     4741 gacacggact cagactgagg ttagctcagt ggaaagatag aactcaccag agcctttgat
     4801 aatgccggta agcttcagaa ttaacccatt ctagagctgc tttacatgtg gacttatgtt
     4861 gatataataa aaacccttca attgtttaag ccacttttaa taggatattg ttagtttttt
     4921 gttggttttt tgttttgttt tgttttgttt tttgtttttt ggagacagtg tcttgccctg
     4981 tcacccaggc tggagtgcag tggtgtgatc ttggttcact gcagcctcga cttcctgggt
     5041 tcaagtgatc cttctgcctc agcctcccaa atagctggga ctataggcat gcatcaccac
     5101 acccagctaa tttctgtatt ttttgtagag acaccgtttc accatgttgc ccaggctggt
     5161 ctcaaaactc ctgtgctcaa gcagtctgcc cgccttggct tcccaaagtg ctgacattac
     5221 aggtatgagc cactgcaccc ggtccctagt agggtatttt taacttggca aaaagggtat
     5281 taatcagaaa catacactat ccatcactga gtattatact cttgattttg caatgtaaga
     5341 gttttaacct atttgtctga cagaaggtgt aaaagtgctg gacatgctgc agaaagaaac
     5401 aggaaaagca tcttgctgca ctccaaagaa tgcaaatttt aatgtgcctt ccaataaatg
     5461 caaggaaacc gaaagagtta atagtatcat acaatgactg ggatgtattg ataatgctac
     5521 agtgatccat taacagcact tgttcaggta ctgctcccaa tcctgggaat gtgtgttacc
     5581 tgttatgtct caaatccttt atcttggctg ttcttctaaa agtgatctgt ctgtcattga
     5641 atgctggcat tagagtcatg tcacaatatt agagcatact ggttttgcct ctaacgtcga
     5701 ggtgaccatc tggtctttct tgctctgtaa gagtttctgt ttgtgctttt ttttccctta
     5761 cttttattat ccagtttaaa tccagtcagt tggccaaaat ggtcttggaa gaaaggattt
     5821 agagattctg taaaaaagat tattagttgg ttctgtgttt gcaaaccagt atgtaattcc
     5881 catatgaatg gggaaaatgt tcctgaggaa ttatttaatt gatgaggcat tcacagagat
     5941 taaagcagtt attgttacct ccaatttatg tcttctgcac agctgattag gttggacatt
     6001 ttaatccaag gtgctactta cattctgttt cttttgttta caaaataggg cattactgag
     6061 tagaaaatac attccaggtc taagaactgt ttgttcttta gtggatttta aagaataaga
     6121 cattaaatta agaaggaaaa tctgtttgat tcatcatttt gagtaatgca gagaataact
     6181 cttaaaaaaa aaacctgaaa cagaaacttt caggaaaaaa acaaaagaaa ttaaactact
     6241 gaataatctt tcaaaagcat cgttgaacag tgagcacatg tgatgccttc ccaattaatt
     6301 aatttgggca aaagctctag ataaacttac accaattgtt gcaaggcatt ctctattact
     6361 gcatttttaa aacaaagtcc tcccctaaaa gttatgaatt atacaaaagc tttaggagga
     6421 attttagtac tcctttaccc aaaataaata gtggtaaatt tccttctgtt cttagctctg
     6481 agtttgtatt tatttataag tcattgtgat catgtatact tatatatttt taatatgcta
     6541 attgctttta aatgggattt ttgttctgat tttaaaataa tacatcctcc ctggacaata
     6601 tttggaaatg tagcaaagta aatcatgtta ccattgttcc attttgatat atataaactt
     6661 taggtattat tttatatata ataaatttta agatattata tacaataaaa ttaaccaatt
     6721 taa
//
