LOCUS       NM_018180               3050 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.
ACCESSION   NM_018180
VERSION     NM_018180.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Hu,X., Yuan,G., Li,Q., Huang,J., Cheng,X. and Chen,J.
  TITLE     DEAH-box polypeptide 32 promotes hepatocellular carcinoma
            progression via activating the beta-catenin pathway
  JOURNAL   Ann Med 53 (1), 437-447 (2021)
   PUBMED   33729094
  REMARK    GeneRIF: DEAH-box polypeptide 32 promotes hepatocellular carcinoma
            progression via activating the beta-catenin pathway.
REFERENCE   3  (bases 1 to 3050)
  AUTHORS   Wei,Q., Geng,J., Chen,Y., Lin,H., Wang,J., Fang,Z., Wang,F. and
            Zhang,Z.
  TITLE     Structure and function of DEAH-box helicase 32 and its role in
            cancer
  JOURNAL   Oncol Lett 21 (5), 382 (2021)
   PUBMED   33777205
  REMARK    Review article
REFERENCE   4  (bases 1 to 3050)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3050)
  AUTHORS   Pant,D., Narayanan,S.P., Vijay,N. and Shukla,S.
  TITLE     Hypoxia-induced changes in intragenic DNA methylation correlate
            with alternative splicing in breast cancer
  JOURNAL   J Biosci 45 (2020)
   PUBMED   31965981
  REMARK    GeneRIF: Hypoxia-induced changes in intragenic DNA methylation
            correlate with alternative splicing in breast cancer.
REFERENCE   6  (bases 1 to 3050)
  AUTHORS   Alli,Z., Ackerley,C., Chen,Y., Al-Saud,B. and Abdelhaleem,M.
  TITLE     Nuclear and mitochondrial localization of the putative RNA helicase
            DHX32
  JOURNAL   Exp Mol Pathol 81 (3), 245-248 (2006)
   PUBMED   16959245
  REMARK    GeneRIF: These results suggest that DHX32 is a putative RNA
            helicase that might be involved in regulating nuclear and
            mitochondrial gene expression.
REFERENCE   7  (bases 1 to 3050)
  AUTHORS   Alli,Z., Nam,E.H., Beimnet,K. and Abdelhaleem,M.
  TITLE     The activation-induced expression of DHX32 in Jurkat T cells is
            specific and involves calcium and nuclear factor of activated T
            cells
  JOURNAL   Cell Immunol 237 (2), 141-146 (2005)
   PUBMED   16414036
  REMARK    GeneRIF: cloned DHX32 proximal promoter and identified a 218bp
            fragment containing two potential binding sites for the
            transcription factor nuclear factor of activated T cells (NF-AT)
REFERENCE   8  (bases 1 to 3050)
  AUTHORS   Meng,X., Liu,J. and Shen,Z.
  TITLE     Genomic structure of the human BCCIP gene and its expression in
            cancer
  JOURNAL   Gene 302 (1-2), 139-146 (2003)
   PUBMED   12527204
REFERENCE   9  (bases 1 to 3050)
  AUTHORS   Abdelhaleem,M.
  TITLE     The novel helicase homologue DDX32 is down-regulated in acute
            lymphoblastic leukemia
  JOURNAL   Leuk Res 26 (10), 945-954 (2002)
   PUBMED   12163057
  REMARK    GeneRIF: Structural characterization and expression of the DDX32
            gene. Expression studies show that DDX32 is widespread but
            specifically down-regulated in acute lymphoblastic leukemias and
            might play a role in normal and/or abnormal lymphopoiesis.
REFERENCE   10 (bases 1 to 3050)
  AUTHORS   Liu,J., Yuan,Y., Huan,J. and Shen,Z.
  TITLE     Inhibition of breast and brain cancer cell growth by BCCIPalpha, an
            evolutionarily conserved nuclear protein that interacts with BRCA2
  JOURNAL   Oncogene 20 (3), 336-345 (2001)
   PUBMED   11313963
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL360176.22.
            
            On May 17, 2019 this sequence version replaced NM_018180.2.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this DEAD box
            protein family are believed to be involved in embryogenesis,
            spermatogenesis, and cellular growth and division. This gene
            encodes a member of this family. The function of this member has
            not been determined. Alternative splicing of this gene generates 2
            transcript variants, but the full length nature of one of the
            variants has not been defined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002473.2, AY064250.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284690.4/ ENSP00000284690.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-750               AL360176.22        115213-115962       c
            751-944             AL360176.22        101660-101853       c
            945-1317            AL360176.22        94273-94645         c
            1318-1560           AL360176.22        88631-88873         c
            1561-1660           AL360176.22        87213-87312         c
            1661-1819           AL360176.22        86963-87121         c
            1820-2011           AL360176.22        76413-76604         c
            2012-2161           AL360176.22        75517-75666         c
            2162-2349           AL360176.22        73671-73858         c
            2350-2531           AL360176.22        72876-73057         c
            2532-3050           AL360176.22        71007-71525         c
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.2"
     gene            1..3050
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /note="DEAH-box helicase 32 (putative)"
                     /db_xref="GeneID:55760"
                     /db_xref="HGNC:HGNC:16717"
                     /db_xref="MIM:607960"
     exon            1..750
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             469..2700
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /EC_number="3.6.4.13"
                     /note="DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32;
                     DEAD/H helicase-like protein-1; huDDX32; DEAD/H box 32;
                     DEAH box protein 32; DEAH (Asp-Glu-Ala-His) box
                     polypeptide 32"
                     /codon_start=1
                     /product="putative pre-mRNA-splicing factor ATP-dependent
                     RNA helicase DHX32"
                     /protein_id="NP_060650.2"
                     /db_xref="CCDS:CCDS7652.1"
                     /db_xref="GeneID:55760"
                     /db_xref="HGNC:HGNC:16717"
                     /db_xref="MIM:607960"
                     /translation="MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFD
                     GLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEY
                     CLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRY
                     CTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSS
                     PHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIV
                     VFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQR
                     RVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQIL
                     GSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRP
                     APESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLT
                     IAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYC
                     VEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGY
                     FMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITS
                     EISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRC
                     TLQ"
     exon            751..944
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1317
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1560
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1660
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1819
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..2011
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2161
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2349
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2531
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..3050
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3026..3031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /note="hexamer: AATAAA"
     polyA_site      3050
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /note="major polyA site"
ORIGIN      
        1 gcagaatcat tttctgaacc agccaggaaa taccggaacc caccaaactt taaacaccag
       61 cctaaattat tcctgttctt ttaagcaggc agcagaaatg acagaaaccc gttaacagaa
      121 aaaaaaaaaa taatgctttt catttgaact cctgtgcatt ttctttttaa cttatatgtg
      181 ttcctaattt tccttactct ttttgtttgt ttgtttctta gtgtggttta ttgacaatca
      241 tttacaatgc cgaagagtgc tgtagtgagc cagcacagtg ggtaacacag caacggagaa
      301 cagatgcagg tttgaggaat ttaacttgct aaaaccttga actgaagtct tagagattgg
      361 aacatacggg tttgtataaa taggctttta agccctgttt gcaatgggtt actgatagga
      421 gaaacttgct tgtggaatgt cagctgcgtg agctcactgt cagacaagat ggaagaagaa
      481 gggctggagt gtccaaactc ttcctctgaa aaacgctatt ttcctgaatc cctggattcc
      541 agcgatgggg atgaggaaga ggttttggcc tgtgaggatt tggaacttaa cccctttgat
      601 ggattgccat attcatcacg ttattataaa cttctgaaag aaagagaaga tcttcctata
      661 tggaaagaaa aatactcctt tatggagaac ctgcttcaaa atcaaatcgt gattgtttca
      721 ggagatgcta aatgtggtaa gagcgctcag gttcctcagt ggtgtgctga atattgtctt
      781 tccatccact accagcacgg gggcgtgata tgcacacagg tccacaagca gactgtggtc
      841 cagctcgccc tgcgggtggc ggatgaaatg gatgttaaca ttggtcatga ggttggctac
      901 gtgatccctt tcgagaactg ctgtaccaac gaaacaatcc tgaggtattg tactgatgat
      961 atgctgcaaa gagaaatgat gtccaatcct tttttgggta gctatggggt catcatctta
     1021 gatgatattc atgaaagaag cattgcaact gatgtgttac ttggacttct taaagatgtt
     1081 ttactagcaa gaccagaact gaagctcata attaactcct cacctcacct gatcagcaaa
     1141 ctcaattctt attatggaaa cgtgcctgtc atagaagtga aaaataaaca ccctgtggag
     1201 gttgtgtacc ttagtgaggc tcaaaaggat tcttttgagt ctattttacg ccttatcttt
     1261 gaaattcacc actcgggtga gaaaggtgac attgtagtct ttctggcctg tgaacaagat
     1321 attgagaaag tctgtgaaac tgtctatcaa ggatctaacc taaacccaga tcttggagaa
     1381 ctggtggttg ttcctttgta tccaaaagag aaatgttcat tgttcaagcc actcgatgaa
     1441 acagaaaaaa gatgccaagt ttatcaaaga agagtggtgt taactactag ctctggagag
     1501 tttttgatct ggagcaactc agtcagattt gttatcgatg tgggtgtgga aagaagaaag
     1561 gtgtacaacc cgagaataag agcaaactcg ctcgtcatgc agcccatcag ccagagccag
     1621 gcagagatac gcaagcagat tcttggctca tcttcttcag gaaaattttt ctgcctgtac
     1681 actgaagaat ttgcctccaa agacatgacg ccactgaagc cagcagaaat gcaggaagcc
     1741 aacctaacaa gcatggtgct ttttatgaag aggatagaca ttgcgggcct aggccactgt
     1801 gacttcatga acagaccagc accagaaagt ttgatgcagg cattggaaga cttagattat
     1861 ctggcagcac tggataatga tggaaatctt tctgaatttg gaatcatcat gtcagagttt
     1921 cctcttgatc cacaactctc gaagtctatc ttagcgtcct gtgaatttga ctgtgtagat
     1981 gaagtgctaa caatcgcagc catggtaaca gctccaaatt gcttttcaca tgtgccacat
     2041 ggagctgaag aggctgcctt gacttgttgg aagacatttt tacatcccga aggagatcac
     2101 tttaccctca tcagcattta caaggcttac caagacacaa ctctgaattc tagcagtgag
     2161 tactgtgtgg aaaagtggtg tcgtgattac ttcctcaact gttcagcact cagaatggca
     2221 gatgttattc gagctgaact cttagaaatt atcaagcgaa tcgagcttcc ctatgcagaa
     2281 cctgcttttg gctccaagga aaacactcta aacataaaga aagctcttct gtccggttac
     2341 tttatgcaga ttgctcggga tgttgatgga tcaggtaact acttaatgct gacacataag
     2401 caggttgctc agctgcatcc cctgtctggt tactcaatca ccaagaagat gccagagtgg
     2461 gtcctcttcc ataaattcag catttctgag aacaactaca tcaggattac ctcagaaatc
     2521 tctcctgaac tatttatgca gctggtacca caatactatt tcagtaatct gcctcctagt
     2581 gaaagtaagg acattctaca gcaagtagtg gatcacctat cccctgtgtc aacaatgaat
     2641 aaggaacagc aaatgtgtga gacgtgccct gaaactgaac agagatgcac tctccagtga
     2701 ctccccagca aacacaaggt gcagcagggt cccaaaggta gctggatggc tgaactgctg
     2761 gatatgggag atacatgacg cgaagacgga tttcacatcc acaggacggt cttgaagaaa
     2821 ataacactgt gtatattatt ttaaaataaa aaatagaagt ttttattgag ttctttaaat
     2881 tactactcca tgcttttctt cttcttggaa aagtttttaa atcaaccact cataatttga
     2941 ccaaaatttt aaaaaactgg tattttgtaa atgtgtcaga gacacatggg acagaaccct
     3001 actttttgta gaggaactta atctgaataa agtctgagtt tttcagtaaa
//
