LOCUS       NP_060650                743 aa            linear   PRI 27-APR-2025
DEFINITION  putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
            [Homo sapiens].
ACCESSION   NP_060650
VERSION     NP_060650.2
DBSOURCE    REFSEQ: accession NM_018180.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 743)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (residues 1 to 743)
  AUTHORS   Hu,X., Yuan,G., Li,Q., Huang,J., Cheng,X. and Chen,J.
  TITLE     DEAH-box polypeptide 32 promotes hepatocellular carcinoma
            progression via activating the beta-catenin pathway
  JOURNAL   Ann Med 53 (1), 437-447 (2021)
   PUBMED   33729094
  REMARK    GeneRIF: DEAH-box polypeptide 32 promotes hepatocellular carcinoma
            progression via activating the beta-catenin pathway.
REFERENCE   3  (residues 1 to 743)
  AUTHORS   Wei,Q., Geng,J., Chen,Y., Lin,H., Wang,J., Fang,Z., Wang,F. and
            Zhang,Z.
  TITLE     Structure and function of DEAH-box helicase 32 and its role in
            cancer
  JOURNAL   Oncol Lett 21 (5), 382 (2021)
   PUBMED   33777205
  REMARK    Review article
REFERENCE   4  (residues 1 to 743)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (residues 1 to 743)
  AUTHORS   Pant,D., Narayanan,S.P., Vijay,N. and Shukla,S.
  TITLE     Hypoxia-induced changes in intragenic DNA methylation correlate
            with alternative splicing in breast cancer
  JOURNAL   J Biosci 45 (2020)
   PUBMED   31965981
  REMARK    GeneRIF: Hypoxia-induced changes in intragenic DNA methylation
            correlate with alternative splicing in breast cancer.
REFERENCE   6  (residues 1 to 743)
  AUTHORS   Alli,Z., Ackerley,C., Chen,Y., Al-Saud,B. and Abdelhaleem,M.
  TITLE     Nuclear and mitochondrial localization of the putative RNA helicase
            DHX32
  JOURNAL   Exp Mol Pathol 81 (3), 245-248 (2006)
   PUBMED   16959245
  REMARK    GeneRIF: These results suggest that DHX32 is a putative RNA
            helicase that might be involved in regulating nuclear and
            mitochondrial gene expression.
REFERENCE   7  (residues 1 to 743)
  AUTHORS   Alli,Z., Nam,E.H., Beimnet,K. and Abdelhaleem,M.
  TITLE     The activation-induced expression of DHX32 in Jurkat T cells is
            specific and involves calcium and nuclear factor of activated T
            cells
  JOURNAL   Cell Immunol 237 (2), 141-146 (2005)
   PUBMED   16414036
  REMARK    GeneRIF: cloned DHX32 proximal promoter and identified a 218bp
            fragment containing two potential binding sites for the
            transcription factor nuclear factor of activated T cells (NF-AT)
REFERENCE   8  (residues 1 to 743)
  AUTHORS   Meng,X., Liu,J. and Shen,Z.
  TITLE     Genomic structure of the human BCCIP gene and its expression in
            cancer
  JOURNAL   Gene 302 (1-2), 139-146 (2003)
   PUBMED   12527204
REFERENCE   9  (residues 1 to 743)
  AUTHORS   Abdelhaleem,M.
  TITLE     The novel helicase homologue DDX32 is down-regulated in acute
            lymphoblastic leukemia
  JOURNAL   Leuk Res 26 (10), 945-954 (2002)
   PUBMED   12163057
  REMARK    GeneRIF: Structural characterization and expression of the DDX32
            gene. Expression studies show that DDX32 is widespread but
            specifically down-regulated in acute lymphoblastic leukemias and
            might play a role in normal and/or abnormal lymphopoiesis.
REFERENCE   10 (residues 1 to 743)
  AUTHORS   Liu,J., Yuan,Y., Huan,J. and Shen,Z.
  TITLE     Inhibition of breast and brain cancer cell growth by BCCIPalpha, an
            evolutionarily conserved nuclear protein that interacts with BRCA2
  JOURNAL   Oncogene 20 (3), 336-345 (2001)
   PUBMED   11313963
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL360176.22.
            
            On Apr 28, 2002 this sequence version replaced NP_060650.1.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this DEAD box
            protein family are believed to be involved in embryogenesis,
            spermatogenesis, and cellular growth and division. This gene
            encodes a member of this family. The function of this member has
            not been determined. Alternative splicing of this gene generates 2
            transcript variants, but the full length nature of one of the
            variants has not been defined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002473.2, AY064250.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284690.4/ ENSP00000284690.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.2"
     Protein         1..743
                     /product="putative pre-mRNA-splicing factor ATP-dependent
                     RNA helicase DHX32"
                     /EC_number="3.6.4.13"
                     /note="DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32;
                     DEAD/H helicase-like protein-1; huDDX32; DEAD/H box 32;
                     DEAH box protein 32; DEAH (Asp-Glu-Ala-His) box
                     polypeptide 32"
                     /calculated_mol_wt=84288
     Region          1..743
                     /region_name="Preny group binding site; CAAX box"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0007744|PubMed:22814378; propagated from
                     UniProtKB/Swiss-Prot (Q7L7V1.1)"
     Region          60..>687
                     /region_name="PRK11131"
                     /note="ATP-dependent RNA helicase HrpA; Provisional"
                     /db_xref="CDD:182986"
     Region          62..237
                     /region_name="DEXHc_DHX32"
                     /note="DEXH-box helicase domain of DEAH-box helicase 32;
                     cd17977"
                     /db_xref="CDD:350735"
     Site            order(62,87..93,129,185..186)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350735"
     Region          146..403
                     /region_name="DEAD/DEXH domain"
     CDS             1..743
                     /gene="DHX32"
                     /gene_synonym="DDX32; DHLP1"
                     /coded_by="NM_018180.3:469..2700"
                     /db_xref="CCDS:CCDS7652.1"
                     /db_xref="GeneID:55760"
                     /db_xref="HGNC:HGNC:16717"
                     /db_xref="MIM:607960"
ORIGIN      
        1 meeeglecpn sssekryfpe sldssdgdee evlacedlel npfdglpyss ryykllkere
       61 dlpiwkekys fmenllqnqi vivsgdakcg ksaqvpqwca eyclsihyqh ggvictqvhk
      121 qtvvqlalrv ademdvnigh evgyvipfen cctnetilry ctddmlqrem msnpflgsyg
      181 viilddiher siatdvllgl lkdvllarpe lkliinssph lisklnsyyg nvpvievknk
      241 hpvevvylse aqkdsfesil rlifeihhsg ekgdivvfla ceqdiekvce tvyqgsnlnp
      301 dlgelvvvpl ypkekcslfk pldetekrcq vyqrrvvltt ssgefliwsn svrfvidvgv
      361 errkvynpri ranslvmqpi sqsqaeirkq ilgssssgkf fclyteefas kdmtplkpae
      421 mqeanltsmv lfmkridiag lghcdfmnrp apeslmqale dldylaaldn dgnlsefgii
      481 msefpldpql sksilascef dcvdevltia amvtapncfs hvphgaeeaa ltcwktflhp
      541 egdhftlisi ykayqdttln ssseycvekw crdyflncsa lrmadvirae lleiikriel
      601 pyaepafgsk entlnikkal lsgyfmqiar dvdgsgnylm lthkqvaqlh plsgysitkk
      661 mpewvlfhkf sisennyiri tseispelfm qlvpqyyfsn lppseskdil qqvvdhlspv
      721 stmnkeqqmc etcpeteqrc tlq
//
