LOCUS       NM_001321708           13469 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant
            2, mRNA.
ACCESSION   NM_001321708 XM_006716172
VERSION     NM_001321708.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Bai,M., Ke,S., Yu,H., Xu,Y., Yu,Y., Lu,S., Wang,C., Huang,J.,
            Ma,Y., Dai,W. and Wu,Y.
  TITLE     Key molecules associated with thyroid carcinoma prognosis: A study
            based on transcriptome sequencing and GEO datasets
  JOURNAL   Front Immunol 13, 964891 (2022)
   PUBMED   36059514
  REMARK    GeneRIF: Key molecules associated with thyroid carcinoma prognosis:
            A study based on transcriptome sequencing and GEO datasets.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13469)
  AUTHORS   Etcheverry,A., Aubry,M., Idbaih,A., Vauleon,E., Marie,Y., Menei,P.,
            Boniface,R., Figarella-Branger,D., Karayan-Tapon,L., Quillien,V.,
            Sanson,M., de Tayrac,M., Delattre,J.Y. and Mosser,J.
  TITLE     DGKI methylation status modulates the prognostic value of MGMT in
            glioblastoma patients treated with combined radio-chemotherapy with
            temozolomide
  JOURNAL   PLoS One 9 (9), e104455 (2014)
   PUBMED   25233099
  REMARK    GeneRIF: the DGKI methylation status identified 22% of poorly
            responding patients in the low-risk group
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13469)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   4  (bases 1 to 13469)
  AUTHORS   Zhang,C. and Pierce,B.L.
  TITLE     Genetic susceptibility to accelerated cognitive decline in the US
            Health and Retirement Study
  JOURNAL   Neurobiol Aging 35 (6), 1512 (2014)
   PUBMED   24468470
REFERENCE   5  (bases 1 to 13469)
  AUTHORS   Troyer,J.L., Nelson,G.W., Lautenberger,J.A., Chinn,L., McIntosh,C.,
            Johnson,R.C., Sezgin,E., Kessing,B., Malasky,M., Hendrickson,S.L.,
            Li,G., Pontius,J., Tang,M., An,P., Winkler,C.A., Limou,S., Le
            Clerc,S., Delaneau,O., Zagury,J.F., Schuitemaker,H., van Manen,D.,
            Bream,J.H., Gomperts,E.D., Buchbinder,S., Goedert,J.J., Kirk,G.D.
            and O'Brien,S.J.
  TITLE     Genome-wide association study implicates PARD3B-based AIDS
            restriction
  JOURNAL   J Infect Dis 203 (10), 1491-1502 (2011)
   PUBMED   21502085
REFERENCE   6  (bases 1 to 13469)
  AUTHORS   Yang,J., Seo,J., Nair,R., Han,S., Jang,S., Kim,K., Han,K.,
            Paik,S.K., Choi,J., Lee,S., Bae,Y.C., Topham,M.K., Prescott,S.M.,
            Rhee,J.S., Choi,S.Y. and Kim,E.
  TITLE     DGKiota regulates presynaptic release during mGluR-dependent LTD
  JOURNAL   EMBO J 30 (1), 165-180 (2011)
   PUBMED   21119615
REFERENCE   7  (bases 1 to 13469)
  AUTHORS   Moskvina,V., Craddock,N., Holmans,P., Nikolov,I., Pahwa,J.S.,
            Green,E., Owen,M.J. and O'Donovan,M.C.
  CONSRTM   Wellcome Trust Case Control Consortium
  TITLE     Gene-wide analyses of genome-wide association data sets: evidence
            for multiple common risk alleles for schizophrenia and bipolar
            disorder and for overlap in genetic risk
  JOURNAL   Mol Psychiatry 14 (3), 252-260 (2009)
   PUBMED   19065143
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 13469)
  AUTHORS   Bex,C., Knauth,K., Dambacher,S. and Buchberger,A.
  TITLE     A yeast two-hybrid system reconstituting substrate recognition of
            the von Hippel-Lindau tumor suppressor protein
  JOURNAL   Nucleic Acids Res 35 (21), e142 (2007)
   PUBMED   17986458
REFERENCE   9  (bases 1 to 13469)
  AUTHORS   Bowne,S.J., Sullivan,L.S., Ding,L., Traer,E., Prescott,S.M.,
            Birch,D.G., Kennan,A., Humphries,P. and Daiger,S.P.
  TITLE     Evaluation of human diacylglycerol kinase(iota), DGKI, a homolog of
            Drosophila rdgA, in inherited retinopathy mapping to 7q
  JOURNAL   Mol Vis 6, 6-9 (2000)
   PUBMED   10706894
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 13469)
  AUTHORS   Ding,L., Traer,E., McIntyre,T.M., Zimmerman,G.A. and Prescott,S.M.
  TITLE     The cloning and characterization of a novel human diacylglycerol
            kinase, DGKiota
  JOURNAL   J Biol Chem 273 (49), 32746-32752 (1998)
   PUBMED   9830018
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022173.7, AC009179.17,
            AC090498.4 and AC078842.2.
            
            On Jun 22, 2020 this sequence version replaced NM_001321708.1.
            
            Summary: This gene is a member of the type IV diacylglycerol kinase
            subfamily.  Diacylglycerol kinases regulate the intracellular
            concentration of diacylglycerol through its phosphorylation,
            producing phosphatidic acid.  The specific role of the enzyme
            encoded by this gene is undetermined, however, it may play a
            crucial role in the production of phosphatidic acid in the retina
            or in recessive forms of retinal degeneration. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.116607.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000614521.2/ ENSP00000479053.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-513               AC022173.7         123323-123835
            514-622             AC009179.17        19673-19781
            623-718             AC009179.17        31023-31118
            719-793             AC009179.17        53135-53209
            794-850             AC009179.17        54887-54943
            851-916             AC009179.17        64138-64203
            917-988             AC009179.17        86121-86192
            989-1105            AC009179.17        89735-89851
            1106-1180           AC009179.17        100066-100140
            1181-1279           AC009179.17        100611-100709
            1280-1362           AC009179.17        109770-109852
            1363-1423           AC009179.17        111768-111828
            1424-1537           AC009179.17        122465-122578
            1538-1675           AC009179.17        124329-124466
            1676-1754           AC009179.17        127747-127825
            1755-1810           AC009179.17        131350-131405
            1811-1873           AC009179.17        132391-132453
            1874-1947           AC009179.17        136837-136910
            1948-2059           AC009179.17        138389-138500
            2060-2259           AC009179.17        157107-157306
            2260-2360           AC090498.4         90287-90387         c
            2361-2440           AC090498.4         56031-56110         c
            2441-2485           AC090498.4         53795-53839         c
            2486-2555           AC090498.4         37971-38040         c
            2556-2596           AC090498.4         35323-35363         c
            2597-2724           AC090498.4         34329-34456         c
            2725-2847           AC090498.4         31910-32032         c
            2848-2873           AC090498.4         12498-12523         c
            2874-2911           AC078842.2         141155-141192       c
            2912-3032           AC078842.2         136860-136980       c
            3033-3069           AC078842.2         126362-126398       c
            3070-3169           AC078842.2         124583-124682       c
            3170-13469          AC078842.2         110022-120321       c
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q33"
     gene            1..13469
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /note="diacylglycerol kinase iota"
                     /db_xref="GeneID:9162"
                     /db_xref="HGNC:HGNC:2855"
                     /db_xref="MIM:604072"
     exon            1..513
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..3286
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /EC_number="2.7.1.107"
                     /note="isoform 2 is encoded by transcript variant 2; DAG
                     kinase iota; diglyceride kinase iota"
                     /codon_start=1
                     /product="diacylglycerol kinase iota isoform 2"
                     /protein_id="NP_001308637.1"
                     /db_xref="CCDS:CCDS94212.1"
                     /db_xref="GeneID:9162"
                     /db_xref="HGNC:HGNC:2855"
                     /db_xref="MIM:604072"
                     /translation="MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAP
                     SAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAA
                     LDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPA
                     KEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVH
                     TACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSF
                     HSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSL
                     KASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTK
                     VLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQL
                     SPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNP
                     DLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAG
                     AAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPG
                     DHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGE
                     PCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFH
                     YDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFP
                     RALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAG
                     TPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSP
                     VSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILD
                     HGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQA
                     GDPDLAAYLESRQNYKVIGHEDLETAV"
     exon            514..622
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            623..718
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            719..793
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            794..850
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            851..916
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            917..988
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1105
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1180
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1279
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1362
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1423
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1537
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1675
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1754
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1810
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1873
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..1947
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2059
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2259
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2360
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2440
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2485
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2555
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2596
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2724
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2847
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..2873
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2874..2911
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            2912..3032
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3069
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            3070..3169
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..13469
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6832..6837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /note="hexamer: AATAAA"
     polyA_site      6852
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
     regulatory      13450..13455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /note="hexamer: AATAAA"
     polyA_site      13469
                     /gene="DGKI"
                     /gene_synonym="DGK-IOTA"
                     /note="major polyA site"
ORIGIN      
        1 ggtgcgggcc ggcgcgggag ggcgcgggag gcggggagcg gcggcctctg attggccggg
       61 gcgggggagt gagcggagtt tcccacaatg ccccggtgcg gtgcagccgc ggatggatgc
      121 tgcgggaagg ggctgccatt tgctgcccct gccagcggcg cgcggacctg cccgcgctcc
      181 tgcagccgcc gccgccgccg ccgccagccc gcccggcccc tgcagcggcg ccgcctgcgc
      241 tccctccgcg gccgccggag cgggcgccat gaaccccagc tcctcggcgg gagaggagaa
      301 aggggcgacg ggcggcagca gcagcagcgg aagcggcgcc gggagctgct gcctgggcgc
      361 cgagggcggc gcggacccgc ggggcgcagg gtcagccgcg gcggcggggg ccgctgccct
      421 ggacgagccc gcggccgccg gccagaagga gaaggacgaa gcgctggagg agaagctgag
      481 gaacttaact ttccggaagc aggtctcgta caggaaagca atctcccggg caggcctcca
      541 gcatctggct cctgcacatc ccctcagcct tcctgtggca aatggtccag ccaaggagcc
      601 cagagcgact ttggactgga gtgagaatgc cgtgaatgga gaacacctgt ggctggagac
      661 caacgtctcg ggagacctct gctaccttgg agaggagaac tgccaagtca gatttgcaaa
      721 atcagctctc aggaggaagt gtgcagtctg taaaatcgtc gtccacaccg cctgcattga
      781 gcagctagaa aagattaatt tcagatgtaa accaacattt cgagaaggag gctcaaggtc
      841 accaagagaa aattttgtac gtcatcactg ggtgcacagg cgtcggcagg aggggaaatg
      901 taagcagtgt ggtaagggct tccagcaaaa gttctccttc cacagtaaag agattgtggc
      961 tatcagctgt tcctggtgca agcaggcgtt tcacaataag gtgacctgct tcatgctgca
     1021 tcacattgaa gaaccctgct ccctgggggc tcatgctgct gttattgtcc cgcccacttg
     1081 gatcattaag gtgaagaaac ctcagaactc cctgaaggct tcaaatcgga agaagaagag
     1141 aacaagcttt aaaagaaaag ccagtaaaag agggatggaa caggaaaaca aaggtcgtcc
     1201 ttttgtgata aaacccatct cttctcctct catgaaaccc ttgcttgtat ttgtgaatcc
     1261 caagagtgga ggcaaccagg gaaccaaagt cctgcagatg ttcatgtggt acctgaatcc
     1321 acggcaagtc tttgatcttt ctcaggaagg gccaaaagat gcgcttgaat tgtataggaa
     1381 agtaccaaat ctgcgaattc tggcctgtgg tggggatgga acggtgggct ggatcctttc
     1441 catcctggat gaactgcagc tgagccctca gcctcctgtg ggggtccttc ctctggggac
     1501 tgggaatgac ctggctcgaa ctctcaactg gggagggggc tacactgatg aacctgtttc
     1561 taagatcctg tgtcaagtgg aagatgggac agttgtacag ctagatcgct ggaacctcca
     1621 tgtggaaaga aaccccgact tgcctccaga agaacttgaa gatggcgtat gtaagctccc
     1681 tctgaatgtt ttcaataact acttcagcct tggatttgat gcccatgtca cactggagtt
     1741 ccatgaatcc agagaagcaa atccagagaa attcaacagt cgttttcgaa ataaaatgtt
     1801 ctatgcaggg gcagcttttt ctgacttcct acagagaagt tctagagatc tatccaaaca
     1861 tgttaaagtt gtttgtgatg gaacagatct caccccaaag attcaggaac tgaagttcca
     1921 gtgtatagta tttttaaata tacccagata ttgtgctggc acaatgccct ggggaaaccc
     1981 aggtgatcac catgatttcg aacctcagcg tcatgatgat ggttatattg aagtcattgg
     2041 atttaccatg gcctctttgg cagccctgca agttgggggc catggagaga ggctacacca
     2101 gtgtcgagaa gtcatgcttc taacttacaa atccatcccc atgcaagtgg atggggagcc
     2161 ctgtaggttg gccccagcta tgattcggat ctccctgagg aatcaggcca acatggtaca
     2221 gaagagcaag aggagaacat ccatgccttt actcaatgat ccccagtctg tcccagatcg
     2281 tctgaggatc cgggtgaaca aaatcagttt acaagactat gaaggattcc actatgacaa
     2341 ggagaaactc cgagaagctt cgatacctct gggtattcta gttgtgcgtg gagactgtga
     2401 tttggagact tgccgtatgt acatagaccg cctacaggag gacctacagt cagtttcttc
     2461 tggctcccag agagttcatt accaggacca tgaaacctcc ttccccaggg ctctctcagc
     2521 acagaggctc tctcctcggt ggtgcttcct agatgcaact tctgctgatc gcttttatcg
     2581 aatagacaga tctcaggaac atttgcactt tgtgatggag atttcccaag atgagatttt
     2641 tattctggac ccagatatgg tggtgtcaca gccggcgggg acacctccgg gcatgcctga
     2701 cctggtggtg gaacaagcct cggggatctc agactggtgg aatcctgccc tgcggaaacg
     2761 catgctgagt gacagtgggc tggggatgat agctccctat tatgaggact cagatctgaa
     2821 agatctcagc cactcccgcg tgctacagtc accagtctct tcagaagatc atgcaatttt
     2881 gcaggcagta atagctggtg atcttatgaa gctaatagaa agctataaaa atggaggcag
     2941 tctgctaatt cagggaccag accactgttc actccttcac tacgcagcta aaaccggcaa
     3001 cggggagatt gtgaaatata tccttgacca cggaccttcc gagttattgg atatggcaga
     3061 cagtgaaacg ggtgagactg cactgcacaa ggctgcctgc cagcggaacc gggctgtgtg
     3121 ccagcttctg gtggatgcag gagcatctct gagaaagacg gactccaagg gtaagacacc
     3181 tcaagaaaga gcacagcagg ctggggaccc agacttggct gcttacctag aaagccgtca
     3241 gaactataag gtcattggcc atgaggacct ggaaactgct gtttgaccct ggtattcggg
     3301 caaagaggac atgagcaagc gtatcacatc tgccctccct gcaattgggc agctcccctg
     3361 gaagaagctg atggaattca tatatctgtc tctctcctgc aagaatctac ctgagaccat
     3421 gccactagct tttaagggct accaagatgt acaacagaac atgatagccc attgagaagg
     3481 aggcaggata cctggagatt tgtggaatac agtacgagtt ccacaaaatt tgatccttat
     3541 tgcttccagc aagtagcatg aacttctgtg ttcacctgta taatttattt taaagattca
     3601 aaggatgttc gtataaatgg cactgctcca tcctccccct atgcattggt ttttttccct
     3661 gtaccataca attctactgt aactacccat caacttaaag aaaaatatta tctcttctct
     3721 ttacattcag tcttggaaga ccacaagatt gtctgaaggc cttctaaaac cttctgaatg
     3781 tcctgcagaa atataactgt aaaaccactt ccatttctaa gactaaatat atcaagacta
     3841 tttagtgact ctctctgcat gtccccctca cccgccaacc ctccgtttca ttatatagga
     3901 gctgggaagt gccacatgga taatgtcaac ttgtgtgcta tatctctgag gaatggtgag
     3961 gtggcatggg agatgtctgt gcttggaggt acctcagaga ggtaacccag gggtcagccc
     4021 aggctgctgg gctgtagcca atagccatgc aggactggtt cagcttgggc tgtctgtaca
     4081 gctccgtact gcctatgtgt agccatcttt gccttttgct gcaatagaag atgagcaaag
     4141 gattaaacag aggcccacag ctagtttgca gaaccactca attttaagtg ctgtttaaat
     4201 tgcagagcaa ataatcctgt gtgggaactg tggttacagg aaatggagca ctctaacaat
     4261 gtttacttct aaactttgtt gaatgataat agaaagcacc ctaattgact tggaaaaaaa
     4321 aaacagcaaa agcaaaagta gcaacatatg tcaacatatg tcactgaaat aggaaacagt
     4381 cattggaatg ttgcacagag gctaataact atagactgtt ggatacagtg gtgagaggag
     4441 ccccatttta ggtctttctt ttaggttttt ggttttcatt actccaagta atccttgacc
     4501 caagaacaaa ggcttgttgt atgagttcca ctgccagatt tatgggatgc ctggatcatt
     4561 cagaaggatg cttcaactat tatttgtcag gtccaaaggt cgtacttaat aaccccattt
     4621 tctatgtatg gggtagtcta atatattatt ttatctactt tatttttccc ttttcagaaa
     4681 gtccttagtg caaaccacca ttggaatcta gccagaaatg tctgtcagat agttagaatt
     4741 gtaacatcta aacctgccac ggatcgaatg gtacttacag gtacctctct tagggactct
     4801 gtgatcccta aaatatcaga agaaaatgtc tgtctttctg tccaaatatc tacttgactt
     4861 gggggtaagt caaaaataaa agtaggtgtc caaggatttt acttttggcg aaatctgacg
     4921 tctcctgagc ttattttcat tttgtccttg gacatagcat agtgcttccc taatgtttct
     4981 acatatgacc atgaatgata gtgtctctac atttaattta aaagtctaat ggtgacattt
     5041 tgcttatctg agtcacattt cggtaacata tcatcatttt gaaagtgcgg ctatgacgtg
     5101 ctatattaaa caggaacagc ctttcagtgg atggggattt tattaccaca gtctttccat
     5161 agtcatttca aaatcaaatt gttggtggga ggatggattg ggttgtgagt aatctacttc
     5221 catatatcag cctcagaaat ggaactatcc tttggatatt acaggagaca ctgcagcaga
     5281 ttgtttccag aagctggaaa aatatgctcc aggagaattt aatcactggg cttttgctgg
     5341 gggattttta gttggcggga ctgtcaagga aatgtgatga cagaaatata atccttaaca
     5401 caaactggaa tctggctttg aatggcatct tccattccct ggattgagtc aaatgtttgg
     5461 ccaagcagtc agttatctag ctgttactgt tgggatgaag aactctgttt tcatcaacta
     5521 ccatctcaca tttctctaaa tacttccaag tagcccagag aaaatttagg gtacaggtgg
     5581 ctatgggcca atttctaaat agctccaatt gaagacataa atctgtgtta tgaaaaatga
     5641 tgagttggta ctgtaagatt cctgatgcat ttggaaattt tttcaaacca aaaaaaaaaa
     5701 aggcattaga gtcctgtgtt attcatggag accaatattt gatatatcgc tttgaaaaaa
     5761 gcaagtacca ctaggacttg ggatttccaa aataaagtga gcagaataac cacatgttta
     5821 atggcttcaa cattaaacca cctcctgtag catgttttaa aaattggtcc tgggctttgg
     5881 aaagagctaa ggccttatgg tctaggagtt tccatctcag cttaagtata ttgtatccct
     5941 actattagga catcaggaaa gcaagtgatc tagtcctggt ctgaagtgcc atgtaacttt
     6001 aacagacata aaaatatgta cctatagaaa tatcatttct acaaagccta ctgagacagc
     6061 actgaagagg cagctagctt ggctgactcc tctgtgtttg tttctttgtg tcatggcact
     6121 gactgctttg agattccatt tttagatgtg gttgatagag gaatggaaaa tgaagagtta
     6181 ttaaaagaca acattctgta acctgaaagg tagtcatttt tgcaagctta tattttaata
     6241 ttgtgcattt aacaaaatag aacacagtta aactgtgctt tatcgtattc aaggacttca
     6301 taaagaataa gagagaagat tagtaagcca agaatggaat aaataccatg actaggaatt
     6361 ttgctttcag agcttatgtt tcctctgtag atgtgataac caaaaaccag tgcaagtttt
     6421 gaaacataat ttagctggca aatgcacagt gatggaaatt agtaatgact gatggagagc
     6481 cgtacatacc tgttgctcct tatcctttca gaatggctgc tttggcagat ctgcctgtgc
     6541 tgggagctac tcaacatcac ttcattttca tctggaccta gtgagagtcc ctttgcctaa
     6601 cgaatttctg aaatttattt atttactaga ccccttataa tattcatggc tttaaaaaat
     6661 gacacagcat ctttatttta ttacatgtca attttattaa taatatttag gggttttttt
     6721 aaattataaa aatggcacat gctttttata aaatgtttta ataccacaga tgagtatcac
     6781 atccacagtc aaggcatccc tttgttctgg tgttgtgtcc cacttcccaa aaataaatac
     6841 ggttaacact gatgaacact gttgcttgta tgtgatgtca gaaatacccc atccgttcat
     6901 gcacaagtat gtatgtatat acgcatatac atttacacac atatatagac atttatttat
     6961 atacacatgc aagtatgtat actatattca tgtatattgc ataggtttag aaaagagcaa
     7021 acattttaaa agcatgctga tcatttgtat atgtctagtg aacaccagta ggcaaagaca
     7081 ggttaaacag agaagttcca tttatttttt tgtttccacc attgctaggc ttagatctgt
     7141 tatggcatta ctacaattgc acttagcatt ttctattacc tgtgtcataa aattcatgac
     7201 caagagttcc tgtaattgtt tatgttgcct tccatgaagc atgaaaccta ccatagcccc
     7261 aagacttatc tatgctatgt ttgtatttca agtaaaggag agttctttcc tctgtgatgc
     7321 ttacaccact ttacaaggct gtttgtaaca ctaacactca gtaggttgcc tagtgatatt
     7381 taattagggc aggctacttc cgggaaggtg taacttcagt gtagtatggg ggtctgagca
     7441 gcttgtcttc cttccagaag gagtagctaa tgaaatcact gagaacagca tattttattc
     7501 aattatgcta tttaaaaatg ggactttgtt aaaatggctc atcataacca tcaaataaaa
     7561 ctactaattg ttctatttaa atgaattata ttcattttag gaaaagaagt agaaaaacat
     7621 ctaataaatt agctttctga ttaatcttta acacttattt aatataaaat ggcttctgcc
     7681 tgcatcttaa ttgaaccacc tttgaatctc tcaactcctt tcactctgaa tctcatactc
     7741 agctgattag aagtcctaca ggtccttctc tgccaggtct gtcagatcga cttggaccct
     7801 acccttttct gtgtccccag tcatccatta tctcttgtct ctctaagttt tgggccatag
     7861 cggcagtcct gtccctgaac tttctgccta ctactgtcaa taattttgtc acgcagttgc
     7921 aaaaatcttc cccagatatt atgctcaaaa tacatcaaaa tgaaattacc ctctgggaca
     7981 agtatacctt ttactcaaag gacacatgga tgtccactga ccttgatcat cttatagaat
     8041 tatggcaagc atgattctga aaatctatca ggtttagcaa atatctgtct tagtcccatg
     8101 agggtacaca atcacggctg ctgcttcctc attcaacaat gtaagccaca tgcctctttg
     8161 acggcacctg gaggactagg ttatagattt tacactgctg agggccatct ttggttttac
     8221 actgtctcct cacagcttaa tgagaaacct gaaaataaga accaagaaaa gagtcatgcc
     8281 cttggacaac ctcctctccc aggacactga gatttactga ccaggtccca gaaagaaagc
     8341 agaaaaataa ttcttaggct cttgtagcat ggattttaaa atagaaacgt gaaaattgaa
     8401 tggtatcttc ataatgcata gactccagag tctatttaca tatgtattca gttatgcatg
     8461 taatcatatg tccatgaatc aatcataagt gcatgtattt aatcattatt tgaaaaatat
     8521 ttgtcactct gaggcaggca tcatactaag gatataacaa tgaatgtaat agacaaaact
     8581 cttgatttca tgaagctggc atctagaaga aaagacaaat tccaatgtga taagtgcagt
     8641 gcaggagaac cccagggtgt ccagcatggg ctggaaattc aaccaaggct ttattgtacc
     8701 agccactcat tagccagcaa tgttttggtt caatgaaaac ataagtagga agagaataat
     8761 attttgcatt tccattttac tggagtcata gtagatttct aaaaatgtat ctgaaactaa
     8821 gattttaaaa caagacttag aaacactatt aaaatgaaag ctaagactga tttattctag
     8881 ctctcaggat agaactagga aaaataagca gagttttcag tgggttttga ctaaatataa
     8941 agaagatgaa gtacaaataa tatgtaatag tggagtggat atcacagtta taaactttca
     9001 gcctctgtta gcatttaaac agaggatacc tgaccactct gaggggtgtt gtgattagga
     9061 atatgtgatt tgagggggct aaaattagat gctgtgattt ttcaaaagat attttttaat
     9121 ggctttgttg aatggcagga ttttatttta aaaactcatg aaaatcttga tttttgattt
     9181 gttaaacttt gctttataac tgagaatttt aaaaatatct aggggaatgg ccgttagtta
     9241 tcatttaaaa aattttaaac taagcatgaa tttaagagct agtcaaaaga aaacatacta
     9301 aaaggtgtaa tttaattaaa aatacttctg gaagcttata aattttgatc agtattctta
     9361 gtcattgtca agtaaaattc tattagaaat ctgtcttact gtccatccaa tataaaaaag
     9421 tgctgtggtt tgatattcta agagttaagt gtaatctagt ataaccacag aagaaaattc
     9481 tgcctgtaag gtatttggga gccaagtaaa tgtacatgaa aggcaatcat ggaaaactct
     9541 ttggcttctg ccaccagcgt ggcaaccaca agggaggtcc tacccatgga aataagaaaa
     9601 tagtgatcca agttttcacc caacttttga aatttcaata ttttgagcca aatgccttct
     9661 gaaatgggag gctttgatag tgggaaatac tgaaattatc ttttccctca gacctttagt
     9721 gaaacacaca tatagttttc actctatgac agtataaaca actcaaatct atactgtttg
     9781 ttaagaacag tttcaagaat taaaaagtgc tttagcgtta tgatgtattg acatgtttga
     9841 gcttcagttg gggttcaaaa caaccccaac gaggtgaata atgcatataa tacattttag
     9901 atgaatgcac tgaatctcag agcagtgaat tcagcctgtg cagattcata tagcaaatga
     9961 tagtgctgca gccagaactg ggtctttaga aattacatat taacatgttt tctaagtaag
    10021 tcattttcca tcattgcctt caaggatgtt ttatatcaat tgttcagatt tccataatat
    10081 agaagttgcc tccatgaaaa aaaaatgttt acctaagttt ccacggaaag aatgagttag
    10141 aaataaggga gaatgtgaaa tcaaaaagtc agagtttttg ctgtcggaga gaggcttgtc
    10201 agcttgtaca tagttcaagc aaagaaaagt ctaatgtttg cataccaggg tggatttcaa
    10261 gagaagaaat taacagtgag cgttaatggc ttctttaact tatttgtgat tctcagcatt
    10321 taaaaaatgt tagtcctatt tttcttcctt tgaagtcact attaggatga tataatttct
    10381 gaatgctgca acaaaaaatg ttaaaaatgg ccctgacatc caagactcta ttgctctaag
    10441 tttatgggtg ggtatgcaag ctaagtgtta tgataataca tagtcatata catagaggtt
    10501 taatacacct atgggaaatt ccggcatatt tgaagtagag aaaaacaaac aggcttgaaa
    10561 tgtccttcag ttcagaatct catactttac aagggacatt tatgggcaga ttggtgaata
    10621 tgtagaagga ggatgtttga tctggaaaga agacaaaatt ggattaggaa taagaatatg
    10681 tagaggagtt aggttaagta ggactgttag tttcaaattc tgcagggaag atgatttgta
    10741 gaataaaata aggaaagaaa agagcagtaa cacatgaaaa ggagttagct ttattactta
    10801 taaggattta tttttcctaa acattgcatc cagaaaaaca gtttgggctt tctgaaaaac
    10861 agtgaagttg ttatcactgt ttcttctagt agttaagtga agattgaaca actaactcag
    10921 agatcaaagg catatcttta attgtgagag atcttggact agaaaaactc tgtgagattt
    10981 tcttggttta aatgtctata attttagact ggaacagaac tcaatttgag cctatttttt
    11041 tcccccaggt tcctgccaac tagatagcaa tgaaggcatt ttttaaaaaa gcttccatca
    11101 ttgcaaatat atcccaaatc acttagcagc ggggatttca tgaccctaaa aagttgcctt
    11161 gcttacacaa acacacctat gctttagtag aaacatatct tttttaggag atttgggaat
    11221 tggctagcat ttttgccaat tgttagccaa tttaggagat ttggaaattg gctagcaatt
    11281 ttgccaattg ttagccaatt cccaaatctc ctaaaaaaga tatgtttcta ctaaagcaaa
    11341 aaggtcacag attcaggagt caaagtgaaa acaaaggatt tagtgttctt tcccctactt
    11401 actacccatt tgttgtttgg gatgtagagt caggactgga gaagagggtg ggaaattcta
    11461 tatcctctgc caatacagag gcatggaggc actttctata actttgtgtc atgaaacaag
    11521 acttaacccc atatcagtag actaatgttt ctgtcatatt tctgtctagg tggattacag
    11581 ttttaattgc ccatcatccc ttaattctgt aatacagctt gagttcctag atttcaggtt
    11641 gactgggtga gggaggaagt cacttgaggt aattttaaga aatgaatcca tatccagagt
    11701 cttgcagaaa agacctgctt ctactccacc ttctttcttg ttctacttct tgcctctggt
    11761 ttgggggcat aaaagagggg cagagttgtg aggaaaggaa aaaagattct caaaagtagt
    11821 acaataagaa gaccctagta gtaacacaaa ttctcatggt aatctcaaac aactgatagt
    11881 gttagcagat attgaaaatc aaactcagga aacatacaga aagattaaat ttgttcaaag
    11941 tggcttcaag gggtattagt tgtgaaaatt tcagtttatg cagcatagaa gaattagatt
    12001 gtgtgtggaa cttgggggaa gtagggtcag ctaatagaag aactttttaa tggagctttc
    12061 caaaggacga ggtggttttt ggaaggagtt tgtccaacct cttataatac attatgcctg
    12121 tccacaaact tgttctggtc cctgataaag tttgaaaaaa ataaggaata tttagggttt
    12181 tttcccacaa atctcaatat attcacttaa aaattcagag actgtgacct tgttattttg
    12241 ggtttaaata tacattttcc tcttaaaaat gatagatata tgtttacaaa ttgtctttac
    12301 tcttcactta taaaacacac acatacagat aggatagtcc tgtcagttta ccctattttt
    12361 tgtttcaaat ttataggtaa tttcaaattt ataggtgttg gtgagattta tcacttttaa
    12421 gataccatct agccttgata tttgttgtac cttctatatg caaccaaatg gctcctgatc
    12481 tcctgatctt ctggagatca aagaattttt attcgccact ctccccaccc atcattggtt
    12541 ggattgagac tctaattaca tacaagcata atttccaatg caagtagaac tttgatgctc
    12601 tccctgactt accgattata agttgggaaa gtttggattc ttgccatcct ggggaaggag
    12661 atttagaact ctcatagaca aaatattcta acacaacaga atattttgca aaacacatct
    12721 ttgaaaagtc aggggccaaa atagtctcag caaaggttag cttggctgtt ttttaaaatt
    12781 accaatagtt aagtgggcaa ccatgaatct gaatattata cagaccacca ctgactgaaa
    12841 ctgaggaaaa ccattcttgt gaaaaactaa ggaatagagg acaatctcag ttctaaaaga
    12901 atgaatatat ttgatttaat ttgatttctg agtgggaaaa cgttcccagg catccagggt
    12961 acccttggct gtgggtattc cactcgcaaa ggctacatct gtatgaagag ttgcagtcac
    13021 attataaagg gctttttcat gatttctgat tctgccctat cacatcccag agccatgctt
    13081 ttgcaaagct gccagctggc aggtggaatc cataaacaag tagcagctgt ggcagtagaa
    13141 agattaaagt tttatttcca ggtaaaggag gattcagata atacgaagtg gagggagggt
    13201 gggtgggggg gggggggtgg gatgggaaag ggaagaatga agttttttgt aacaaagtca
    13261 taaagtattt ttccctaatt ttcttgcatt tcctatttat ataaagtatc ttgatcactg
    13321 tgttgcacta cagagatttt caatggagtt aatttgggag actgaactaa tctctgaaaa
    13381 agaaattgac tagttacagt tactttttgg acactttttc tgtattgtga attgtgtgga
    13441 aatgtacaga ataaaaatga agaaatgta
//
