LOCUS       NM_178009              17497 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant
            2, mRNA.
ACCESSION   NM_178009
VERSION     NM_178009.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Sharma,P., Yadav,S.K., Shah,S.D., Javed,E., Lim,J.M., Pan,S.,
            Nayak,A.P., Panettieri,R.A. Jr., Penn,R.B., Kambayashi,T. and
            Deshpande,D.A.
  TITLE     Diacylglycerol Kinase Inhibition Reduces Airway Contraction by
            Negative Feedback Regulation of Gq-Signaling
  JOURNAL   Am J Respir Cell Mol Biol 65 (6), 658-671 (2021)
   PUBMED   34293268
  REMARK    GeneRIF: Diacylglycerol Kinase Inhibition Reduces Airway
            Contraction by Negative Feedback Regulation of Gq-Signaling.
REFERENCE   2  (bases 1 to 17497)
  AUTHORS   Matsumoto,Y., Suzuki,A., Shirata,T., Takahashi,N., Noto,K., Goto,K.
            and Otani,K.
  TITLE     Implication of the DGKH genotype in openness to experience, a
            premorbid personality trait of bipolar disorder
  JOURNAL   J Affect Disord 238, 539-541 (2018)
   PUBMED   29936393
  REMARK    GeneRIF: The present study shows that a bipolar-risk allele of DGKH
            is associated with higher openness to experience, providing further
            evidence for the implication of this gene in the etiology of
            bipolar disorder.
REFERENCE   3  (bases 1 to 17497)
  AUTHORS   Weissflog,L., Becker,N., Bossert,N., Freudenberg,F.,
            Kittel-Schneider,S. and Reif,A.
  TITLE     Expressional profile of the diacylglycerol kinase eta gene DGKH
  JOURNAL   Eur Arch Psychiatry Clin Neurosci 267 (5), 445-454 (2017)
   PUBMED   27085324
  REMARK    GeneRIF: This sstudy shown that the highest expression levels of
            DGKH in the human brain were found in the striatum.
REFERENCE   4  (bases 1 to 17497)
  AUTHORS   Gregersen,N.O., Lescai,F., Liang,J., Li,Q., Als,T.,
            Buttenschon,H.N., Hedemand,A., Biskopsto,M., Wang,J., Wang,A.G.,
            Borglum,A.D., Mors,O. and Demontis,D.
  TITLE     Whole-exome sequencing implicates DGKH as a risk gene for panic
            disorder in the Faroese population
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 171 (8), 1013-1022 (2016)
   PUBMED   27255576
  REMARK    GeneRIF: several single variants and genes showed strong
            association with Panic Disorders, where DGKH was found to be the
            strongest Panic Disorder associated gene. Interestingly DGKH has
            previously demonstrated genome-wide significant association with
            bipolar disorder as well as evidence of association to other mental
            disorders.
REFERENCE   5  (bases 1 to 17497)
  AUTHORS   Yasuda,S., Kai,M., Imai,S., Takeishi,K., Taketomi,A., Toyota,M.,
            Kanoh,H. and Sakane,F.
  TITLE     Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf
            heterodimerization
  JOURNAL   J Biol Chem 284 (43), 29559-29570 (2009)
   PUBMED   19710016
  REMARK    GeneRIF: DGKeta acts as a novel critical regulatory component of
            the Ras/B-Raf/C-Raf/MEK/ERK signaling cascade via a previously
            unidentified mechanism.
REFERENCE   6  (bases 1 to 17497)
  AUTHORS   Tesli,M., Kahler,A.K., Andreassen,B.K., Werge,T., Mors,O.,
            Mellerup,E., Koefoed,P., Melle,I., Morken,G., Wirgenes,K.V.,
            Andreassen,O.A. and Djurovic,S.
  TITLE     No association between DGKH and bipolar disorder in a Scandinavian
            case-control sample
  JOURNAL   Psychiatr Genet 19 (5), 269-272 (2009)
   PUBMED   19478689
  REMARK    GeneRIF: No significant association after multiple-testing
            correction between any of the single nucleotide polymorphisms in
            DGKH and bipolar disorder, was found.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 17497)
  AUTHORS   Baum,A.E., Akula,N., Cabanero,M., Cardona,I., Corona,W.,
            Klemens,B., Schulze,T.G., Cichon,S., Rietschel,M., Nothen,M.M.,
            Georgi,A., Schumacher,J., Schwarz,M., Abou Jamra,R., Hofels,S.,
            Propping,P., Satagopan,J., Detera-Wadleigh,S.D., Hardy,J. and
            McMahon,F.J.
  TITLE     A genome-wide association study implicates diacylglycerol kinase
            eta (DGKH) and several other genes in the etiology of bipolar
            disorder
  JOURNAL   Mol Psychiatry 13 (2), 197-207 (2008)
   PUBMED   17486107
  REMARK    GeneRIF: diacylglycerol kinase eta (DGKH) is association in the
            etiology of bipolar disorder.
            GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 17497)
  AUTHORS   Murakami,T., Sakane,F., Imai,S., Houkin,K. and Kanoh,H.
  TITLE     Identification and characterization of two splice variants of human
            diacylglycerol kinase eta
  JOURNAL   J Biol Chem 278 (36), 34364-34372 (2003)
   PUBMED   12810723
  REMARK    GeneRIF: alternative splicing of the human DGK eta gene generates
            at least two isoforms with distinct biochemical and cell biological
            properties responding to different cellular metabolic requirements
REFERENCE   9  (bases 1 to 17497)
  AUTHORS   Sakane,F. and Kanoh,H.
  TITLE     Molecules in focus: diacylglycerol kinase
  JOURNAL   Int J Biochem Cell Biol 29 (10), 1139-1143 (1997)
   PUBMED   9438377
  REMARK    Review article
REFERENCE   10 (bases 1 to 17497)
  AUTHORS   Klauck,T.M., Xu,X., Mousseau,B. and Jaken,S.
  TITLE     Cloning and characterization of a glucocorticoid-induced
            diacylglycerol kinase
  JOURNAL   J Biol Chem 271 (33), 19781-19788 (1996)
   PUBMED   8702685
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157932.21, DR002010.1,
            AB078968.1, AK302727.1, BC043292.2, AL136527.9 and BM978856.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_178009.4.
            
            Summary: This gene encodes a member of the diacylglycerol kinase
            (DGK) enzyme family. Members of this family are involved in
            regulating intracellular concentrations of diacylglycerol and
            phosphatidic acid. Variation in this gene has been associated with
            bipolar disorder. Alternatively spliced transcript variants have
            been identified. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) contains an alternate exon in
            the 3' coding region, which results in a frameshift, compared to
            variant 1. The encoded isoform (2) has a longer and distinct
            C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB078968.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337343.9/ ENSP00000337572.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                AL157932.21        39986-40045
            61-105              DR002010.1         9-53
            106-3452            AB078968.1         1-3347
            3453-3805           AK302727.1         3051-3403
            3806-4354           BC043292.2         3409-3957
            4355-16928          AL136527.9         126827-139400       c
            16929-17497         BM978856.1         16-584              c
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.11"
     gene            1..17497
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /note="diacylglycerol kinase eta"
                     /db_xref="GeneID:160851"
                     /db_xref="HGNC:HGNC:2854"
                     /db_xref="MIM:604071"
     exon            1..318
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..3789
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /EC_number="2.7.1.107"
                     /note="isoform 2 is encoded by transcript variant 2; DAG
                     kinase eta; diglyceride kinase eta"
                     /codon_start=1
                     /product="diacylglycerol kinase eta isoform 2"
                     /protein_id="NP_821077.1"
                     /db_xref="CCDS:CCDS9381.1"
                     /db_xref="GeneID:160851"
                     /db_xref="HGNC:HGNC:2854"
                     /db_xref="MIM:604071"
                     /translation="MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAE
                     QEGPQKLIRKVSTSGQIRTKTSIKEGQLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKS
                     LIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENRKEMEDWISSLKSVQTRE
                     PYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCA
                     VRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQD
                     WKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLL
                     VFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDG
                     SVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERAS
                     TKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVV
                     ISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQ
                     AAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSL
                     KKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTA
                     PRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINK
                     MLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKC
                     RSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGG
                     TNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIF
                     GDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVL
                     RTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHA
                     LNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSV
                     EVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQ
                     PVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKDLGIPKVGHVKRI
                     LQGIKELGRSTPQSEV"
     exon            319..429
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            430..510
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            511..615
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            616..748
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            749..855
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            856..981
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1084
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1244
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1353
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1493
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1578
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1664
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1764
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..2038
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2161
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2293
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2411
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..2522
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2619
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2727
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2841
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..2976
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3140
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3246
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3339
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..3459
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3568
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3569..3699
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3700..17497
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      17473..17478
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /note="hexamer: AATAAA"
     polyA_site      17497
                     /gene="DGKH"
                     /gene_synonym="DGKeta"
                     /note="major polyA site"
ORIGIN      
        1 gggaaacgga agatggcggc ggcggccggg cacggggttc cgggctccgc tcgggcagag
       61 cccacccgct gaccaacgcc gccgcccccg ccgggcggtg ctgtgtcccc gcaggagtcg
      121 gagaggatgg caggggccgg aggccagcac caccctccgg gcgccgctgg aggagcggcc
      181 gccggagccg gcgccgcggt cacctccgcc gctgcctcgg cggggccggg agaggattcg
      241 tctgacagcg aagcggagca agagggaccc cagaaactga tccgcaaagt gtctacctcg
      301 gggcagatcc ggaccaagac cagtattaaa gagggacagc tattgaagca aaccagttct
      361 ttccaaaggt ggaaaaagcg atacttcaaa cttcgaggcc gcacccttta ctatgcaaag
      421 gactcaaagt ctctgatatt tgatgaagtt gacctctcag atgctagtgt agctgaagca
      481 agcacgaaaa atgctaacaa cagcttcacg atcatcactc cattcagaag gctaatgctg
      541 tgtgctgaga acagaaagga gatggaggat tggatcagct cactgaagtc tgtacagacc
      601 agagaaccct acgaggtggc ccagtttaat gtggaacatt tctcagggat gcacaactgg
      661 tacgcctgct cccacgcccg acccaccttc tgtaacgtgt gcagagagag tctttctgga
      721 gtcacctccc atggcctgtc ctgcgaagtg tgtaaattca aggctcacaa aagatgtgca
      781 gtgagagcaa caaataactg taaatggact accctggcct ccatcgggaa ggacattata
      841 gaagatgaag atggcgtcgc gatgcctcac cagtggcttg agggcaacct gcctgtaagt
      901 gccaagtgtg ctgtctgcga caaaacatgt ggcagtgttc tccgtctaca ggattggaaa
      961 tgcctttggt gtaagacaat ggtacacact gcctgcaaag atttatacca tccaatatgt
     1021 ccacttggtc aatgtaaagt atctatcata cctccaattg cactaaacag caccgattcc
     1081 gatggtttct gtagagcaac attttcgttc tgtgttagtc ctctattggt ttttgtcaat
     1141 tctaagagtg gagataatca gggagtaaag ttcctccgtc gctttaaaca gttgctaaat
     1201 ccggctcagg tgtttgattt aatgaatgga ggtcctcatt taggtttaag attatttcag
     1261 aagtttgaca atttccggat tcttgtttgt ggaggcgatg gaagtgtagg ttgggttttg
     1321 tcagaaatcg ataagctcaa cttgaataaa cagtgtcagc tgggagtgtt gcctttgggt
     1381 acaggaaatg accttgcccg agttcttggc tggggaggtt catatgacga tgacacccaa
     1441 cttcctcaga tcctagagaa actggaacga gccagtacca aaatgttgga caggtggagt
     1501 ataatgacat atgaactcaa attgccacca aaagcttccc tacttccagg acctccagaa
     1561 gcatctgaag aattttatat gacgatttat gaagactcag ttgcaacgca tcttacaaaa
     1621 atcctcaatt ctgatgaaca tgcagtggtc atatcttctg ccaagacgct atgtgaaact
     1681 gtaaaggact tcgttgccaa agtagaaaag acgtatgaca aaaccttgga aaatgccgtt
     1741 gtagctgatg ccgtggccag taaatgttca gtcctaaacg agaagctcga acaactgctg
     1801 caggctttgc acacagattc ccaggctgcg cctgttctcc ctggcctcag ccctctcatt
     1861 gtggaagaag atgctgtgga atcgtccagt gaagagtccc tgggtgaaag caaagagcag
     1921 cttggggatg acgttacaaa accttcctcc cagaaagccg tcaaaccaag ggaaatcatg
     1981 ttgcgggcaa atagtttaaa gaaagcagtg aggcaagtca ttgaggaagc cggaaaagtt
     2041 atggatgacc cgacagttca cccctgtgaa ccagctaatc agtcctctga ttatgacagc
     2101 acagaaacag atgaatctaa ggaggaagct aaagatgatg gtgccaaaga atcaataact
     2161 gttaaaactg cacctcggtc tccagatgcc cgggcaagtt atggccattc ccaaactgat
     2221 tctgtccctg gtccagctgt ggcagccagc aaagaaaacc tccctgtgct caataccaga
     2281 ataatctgcc caggtttaag agcaggactg gctgcctcaa ttgctgggag ttcgattatc
     2341 aacaaaatgt tactggcaaa cattgatcct tttggtgcca cgccgtttat tgacccggat
     2401 ctagattccg tagatggata ttcagaaaaa tgtgtcatga acaattactt tgggattgga
     2461 ttagatgcaa aaatttcatt agaatttaat aataaaagag aggagcaccc tgaaaaatgc
     2521 aggagccgaa ctaaaaactt gatgtggtat ggagtccttg gaacccggga gttattacag
     2581 agatcgtaca agaatttaga acaaagggtt caacttgagt gtgatgggca gtatattcct
     2641 cttcccagct tgcaaggcat agccgtgttg aacattccca gctatgctgg aggcactaac
     2701 ttttggggtg gaactaaaga ggatgatata tttgctgcac catcctttga tgacaagatc
     2761 ctggaagttg tagcaatatt tgatagcatg caaatggcag tttcaagggt cattaaactg
     2821 cagcatcatc gaatagccca gtgccgtaca gtgaaaatca ctatatttgg tgacgaagga
     2881 gtcccagtgc aagtggatgg tgaagcgtgg gttcagcctc cagggattat caaaattgtg
     2941 cacaaaaaca gagcacaaat gctaacaagg gacagagcct ttgagagcac tctgaaatct
     3001 tgggaagata agcagaagtg tgattctggt aaaccagttc tccgaaccca tttgtacatc
     3061 catcacgcca ttgacttggc aacagaagag gtgtcgcaga tgcagctatg ctcccaggct
     3121 gcagaggagc tcattactag gatatgtgac gcagccacaa ttcactgtct tttggagcaa
     3181 gaactggccc atgctgtgaa tgcctgctcc catgccctga ataaagccaa cccaaggtgc
     3241 ccggagagtc ttacaagaga cactgccact gaaatagcca tcaatgtgaa ggcgctgtat
     3301 aatgaaacag aatctttgct agttggcagg gttcctttgc agctggaatc gccacatgaa
     3361 gagcgagtat ccaatgcctt acactctgtg gaggtggaat tacagaaact gacagagatt
     3421 ccttggcttt attatatctt acacccaaat gaggatgagg aacctcctat ggattgcacc
     3481 aaaaggaaca acagaagcac cgtatttcga atagtgccaa agtttaaaaa ggaaaaggtt
     3541 cagaagcaga agacaagttc acagcctgtt cagaaatggg gcacagagga agttgctgct
     3601 tggctggatc tgctcaattt gggagagtac aaagatatct tcatccgtca tgacatcaga
     3661 ggggctgaac ttttgcatct ggaaaggcga gatcttaagg atctggggat accgaaagtg
     3721 ggtcatgtga agcgaattct ccagggaatt aaagagcttg gaaggagcac tccacagtcg
     3781 gaggtgtaat catattggtg ctatttcttg gaagagaagt tattgccact taatacaaag
     3841 tccttggaag caagtggctg ttcttgtagt tttctgcata gataagtaag caccactgaa
     3901 gcacctctgt ggcttgatat tttgctgtgg gtgaaatttt gatttgaggt attagaaaat
     3961 atttttgtgc cgaacaatac attccacaaa gccattttct ttttgtgcaa acctgacatg
     4021 ttcaaatata ttcacaatgg taataaggta ggaggaatct gagacgattg cattgtctaa
     4081 acagtggaat tgcaaaatgt ttactttcca tagatctggt ctgatggaat gttcctactg
     4141 tgcaactgca taacaatatg tggttaaaac ttcctaggtt tcattacggg ggggcattgg
     4201 ccattttctt aaaatcatag gttaggagac taaaatataa attaagttgt gatttgaatg
     4261 tagattggta tcacctccaa ctcctagtgc ttagtggtca aatatgttga aaacctttcc
     4321 aatactatga atgaatgcaa atcttaatgc acgatgttcc tgcctgaaat gtctttcttt
     4381 ttcttgcatg tcacatctaa tactgtaaca ctttaaatag ctttcatgtc agttttaatg
     4441 ttaatgggat ttaatttatt actaaagtac atttttgttg gttaagggca ctgcactttt
     4501 ctgtttttta atgtgggatt ttgcgtaaat attctgtacc taagactttg tgaaagtgtg
     4561 tctgtgccat aatcaacaaa tgatatacat cacatgcaag tcaattttat ttcaatctat
     4621 cataagcagt aggcgtgcac gttaagaaaa ttctctggat attttcataa aattacatgc
     4681 tcccttacct gacaaaactt gttacagctt tccaaaccaa aatttccaca ttttgtttct
     4741 aatttttaat ttcaatttaa taattattta atattctatt tgaatttata atatagtaaa
     4801 gtgttgatat ccaggaattc cctgaggtgt caactgagat ttatatactg acagataacc
     4861 cagactcacc cgctctctac agtgacttct gacacgatct tccaaacaga agggatttaa
     4921 attttttctt aagggcttta tgttgttatt attattatct cacttaattc ttaattctta
     4981 tcaggtggat attattttcc tttttctaaa ccactgtact tcagtgtagc ttattattag
     5041 aagtcaacat tacaatgatc aaatctttga aagccaaagg catacgtatc aaaaagtcac
     5101 aaaaatatcc ttaaataagc tttctttagc cattatatac caaaacatta attataatat
     5161 tttaaaattt gtatataaga gaaataaaca gggtactgac tcagtggtga caatttttat
     5221 gaagtagcct gctaggtgct taatagatat atatacacac agatatatac atatacacat
     5281 atatgtgtat atatacatac atacacatat acatttatat gtgtatatat atacctacat
     5341 acatataacc ttgtgatatg gcatcattga tattttctag acaattattt ccccagtgtt
     5401 taaagagaaa tactaatagg cgagcattta aaatgaacta ttgtcaggca tattatttaa
     5461 ttacatcaaa ggagtttaac tgtttgacac tttactcttg tttataactt gggtatttaa
     5521 ctactagtaa aaatcacaaa attttaatgt ctttaaaata aatgaccctg agggattcct
     5581 gaccaagtat gtctgactat atattttacc taagttgtaa ataaatggaa attttccaaa
     5641 gacagttata tcaagtagtt taaaaagtca tgaggcaggg tgctgtggct cacgcctgta
     5701 atcccagcac ttcgggaggt ttaggtgggt agatcaccgg aggtcaggag ttcgagacca
     5761 gcctgaccag tgtatgaaac cccatctcta ctaaaaatac aaaaattagc cgggtgtggt
     5821 ggcacgcacc tgtaatccca gctactcggg aggctgaggc aggagaattg cttgaaccca
     5881 ggagatggag gttgcagtga ctgagatcac accactgcac tctactagcc tgggtgacag
     5941 agcaagactc ccatctcaat ctcaaaaaaa aatagtcatg aattatgcaa ttattctaat
     6001 tgtatctgca cttacgaagt cagaggtctc ctttttcagt ccctctgata gtcccaccta
     6061 agtgctgtcc tgctcctgtg aacactttcc tgtgcaacca aacaggaggt gccaggttcc
     6121 cagaactctc taacattaca gatctctgtc catctttact aaaaaaagga aatgtgaggg
     6181 aaagaagggg aagaactcta gcataaataa tgtattaata taaatatcta tattgatttt
     6241 caccccccat tcccctttgc taatagaaaa gagtctagct atgatgtgtc ccaaatttaa
     6301 gagtgtgatg tgaaggactg aatttttgtt tgttcgtttg gtgagctttt cactcgtagg
     6361 tgacatcttt tgtatatgct gtaaagtctc agttaatgga atcctacagt gcatttctgg
     6421 ctagctcggt tggtagagcg tgagtctctt aatggaatcc ttaattagct agaattttcc
     6481 tgcaaatggc tttggatgag ggaaattcta taaaatgaca atttggatat actttttgtt
     6541 aaatgagagc aattttctct gtatgctttg tctatgcatg gctgtgcagg gcccaaatga
     6601 cttcactgac acctgtttcc aggagggggt cttcagagcc tccttaggct cttgtcagta
     6661 tcataaataa ttcctcttct tatagcggat ggggttacca aaaaaaataa aaagtaggga
     6721 agagcttctc catgggatca gatcacagct ttggggagtg ggatggggcc tctatgcttt
     6781 gatctatgca tgagctgggg tgagaagttc ccacctgcac ctgactgcaa tgactgtaat
     6841 ggctggaatt gtcacctctt ctccctgata ggctcttcct cacctggagg atcggggctg
     6901 gtgggagggc agggaagggg aggccatcag ccagaatgta ttttctttca gttccatttc
     6961 ctgaggaaga acttgctttt aaaggaggaa agggacagaa tcagacctaa ataaaattcc
     7021 tgagtatgca atatttctct ttagtgtctc ttcacctccc tttctgagtt tctttcctac
     7081 atcctggctc ttcattttcc caatgcccaa tataatcatg gttgctgaaa ttctcactgc
     7141 cttctacttt cctttcccca tcaattaacc cactacaaaa tgaccccttt catttctcgc
     7201 ttaagcatac atggtgggtc atttaactaa gtgttaggaa gaaaacaatt taaagacact
     7261 aattttacag tttgcgttct gtaaaaggat acttgctgga taatctaagt gaattgatgg
     7321 attaggtcat ttgggattgt gatatatatt gttcttgctg acaagaaatg acccatgaat
     7381 gtctagatct atcctgtcca atattgtagc tacatgtggc tatctaaatt taaatgtaat
     7441 ttaattaagc caggcataat ggcacaagcc tgtaattccg gtgactcagg aggcttagga
     7501 gggaagatgg cttcaggcca ggagtttgag accagcctcg gcaacatagt gagaccctgt
     7561 ctctgaaaaa aaattttttt aactagctag gcatggtggc atgcgcctgt agtcccagct
     7621 attctgaagg ctgaggtggg aggattgctt gagaccagga gttcaaggct gcagtgagcc
     7681 atgatcatgc tactgccctc cagcctgggc agcagagcaa gacccctact ctttaaaaaa
     7741 attaatttaa ttagaaactc catttctcag ttgcactaac tacatttcaa gtgttcagta
     7801 accatagtgg acagtgcaga tgtagaaaat ttccatcact gcaaaaattc tattggacag
     7861 cactggtgca gatcattgct actcaaaatg tgatccatgg gccagcagca tcaatattac
     7921 ctgggagctt acagaaatgc agaatttcag gcccactgca gatctaccga atcaaaatct
     7981 tcctttagca aaatttctca aacgattagc actggcctac atccatttta tccttcctta
     8041 gctattaggg atgtgaggtc cgagggcttc aaaaggtccc cggaatagct tgttccttca
     8101 tccactgtgt cctattcatt cttcagctaa ctccagcaat gagctgaaac tcattcatca
     8161 cccttgctga gttttcttct caatccttat tcctaattct ggttctagat gagccctacc
     8221 tacccagtgg ttgtattttt gtagccagtg tgggacacag gagattggca gaccaacaca
     8281 gctagcctct ctctagccct ccctccacct ctaagtcact aacaatccat gtttgttcag
     8341 tttgttgaca tgtggcatgt tcatttgttc acaacttaat cacgggggac atttcagaaa
     8401 aatgtgtact aagttaaaac catgtttagt ctcctacaac ttgtacattt tcattttctc
     8461 ttatcagtag attgtccttg ttgacatagc tcatgcatga ggacacatag cagtacacac
     8521 acattgaatg aattgttagt catgcaaaaa aagaaaagga atgaaccaag atgattgcaa
     8581 gttaatctca tttaagccct ctaaaaaggc atgctttgct agatttagat ctgttttcct
     8641 tacactcact tatcttgatg ttcacatggt taataattcc ttaaaatagt gcctgaagaa
     8701 taatatttca tattcttgac ccccaaataa cagtttactt aattttataa gatttttccc
     8761 aatgagactt attttcagga atccttctct taataacttt tacatattga tataatgtac
     8821 caaaacacaa ttgcctagag atgctttagc taatttttat taataaagag gacaatattt
     8881 ttgtcttcat ggaacttatt tttaacagga atcaaggaag aaaatattca tgcagagcta
     8941 tgacttagaa agttataggg gctgccacta tacagcactt ctaaagtaaa acatcttcta
     9001 tctccaactg ggctgtcaac atagtggact ttcatgttct atggatatga attctttaag
     9061 gatgtagccc ttaattaagc tgtaaaaaca atgcatctct caaccttgac atttaggatc
     9121 tctcctgtct gccccctcta cgccccactc caattaactc atcctacatc tctcagatgt
     9181 aagttggata agagtgggtt tgaagtttgg taatacaagt ggcagtttta aaacaccaaa
     9241 ttctttagat cagctgaaaa gaagagtgag aattgaaaat tcctccacta gtaattgtta
     9301 gccaatagaa tagcctccca gagcagtccc cacagcactt ggttgccatg ggtttctctc
     9361 ctctcactgc ctgccccagt gccatgatgt ctggggcttg tctcttctat ggcatcccct
     9421 gattgacttt tgtatagcaa cctctgtgtc aagtcagtaa tatgatgcca ttgtaatagg
     9481 aggccttcct aaagaagtaa ctgcaacttt taaaacgtga tgattattgg tttttataat
     9541 acacaaagca ttaatttttt cttatcttat tttctcttta ttatttcttt ccaacttttg
     9601 tttggagaca tttatgttag aagaaaaact ataaagtttt atcattgtag agaacagaga
     9661 gatcaaagac agctggactg tacctgtttg tgtaattatt tctgatattt ttaggaaaag
     9721 aagtttaaca gagtaactca gactgagact gttaaactct gttcatgata aactccatat
     9781 tacataatat aggtgaacag attaattccc tccatgtaac taagatactc tatttacatt
     9841 atttatatac tctcttagtt ttttatgcac ttccaagttt tagttggaaa aatgaaaacc
     9901 aagctactac ttaaaagtta ttaaaaactt cacataaaat atttcatttt agctacaagt
     9961 tattcattgc ataggatttt ataaatagtc tgggtatttc ataggaactg catttcaaaa
    10021 gtgaattaac caaacctaaa aaaagggtta cttttaaaat gcattaaaat aaagaagttt
    10081 catattcagt tgataaggcc attaaggtag gataatgcag ttacatatac aaactaaaaa
    10141 aggaaaaaga atatattttg tggtccttaa tgtttgattg cactattgtt tcccacagct
    10201 tagccgtatc tatttctgta taccaagtat taagctgata tgtagctaaa tatttcttac
    10261 agttctggta actggtaact agtgctgtga aaggttttgg tattgtaaca ttcccagttt
    10321 tgtgagttat atattgtata ttgaagatga ctagtaaatt caagtaatat atttatctac
    10381 agctgggaaa atataattat tttcaaatct tcaagtaagt tgtatcgaat gtttttcttc
    10441 ctcttaagtg agataaatct cacttttatg ttaaaatgtc tatttataca acaggtttct
    10501 ccttttgctg ttattttcat attaaaattt ttcttatctt tttatatcaa aataattatg
    10561 ctggctattc aaactgccaa gatgtatttt agtttgcagg atttttggca gacaaaatat
    10621 ttaataacaa atataaaagc tttgtatatt ttgtggcatt aggggcaaga attttacctt
    10681 tggatttctc tatgtgaggc agtgctggtg tcagcaaagt cagtaacaga cagtattact
    10741 tgtaatcatc aaaagaaggt ttccacaagt tgtataaatc agtgcattta tttattatgt
    10801 tgtgaaactt aagctttata tgaaatacat attctagctc ttttcctttt tactgctgtt
    10861 gtttagagtg tgtagttctg ttgatgattt acttatgtac ttgaagtact catttctgtc
    10921 aaacagatgc ccaactattt tccttttaaa aaactgtatt ctttactgca atagatagcg
    10981 gaatagtaat agacactttg ttaaaaaaat aagtttcatg tttaaaataa atattttttt
    11041 aaaatataaa gccaaaatga attcaccaat attaaggatt taatgtagcc aagggattca
    11101 gtgtgttata ttttgccaat atgtatttta tcttcatgtc acaacttatt ttctgtagcc
    11161 aattctcagt tttagttgaa gagagatctc agtgattgta ctgccaatat gacattcaaa
    11221 tgaacattag gaatcttgaa ccatggctga gcgctgttgc tcaggcctgt aatcccagca
    11281 ctttggaaag ccgagggagg tagatcacct gaggtcagga gtttgagacc agcctggcca
    11341 acttaataca aaaattagcc aggtgtggtg gtgggtgcct gtaatcccag ctactcagga
    11401 ggctgaggca ggagaattgc ttgaacccag gaggtggagg ttgcagtgag ccaagatctc
    11461 gccactgcac tccagcctgg gcgacagagc gagactctgt ctcaggggga aaaaaaagaa
    11521 ttttgaaccc cccaaaatat cacatatcta gcagttctag gttccagggt ggatattttt
    11581 aacatatgtt tgcatttggt tctgggatag taaccgttat ggataattgt ttgtttctgt
    11641 caagtcggtg agattttaat tgtttgaatt atttctattt aaaattactc tcaatttaac
    11701 tgtttaaaaa actaaaaatt taaaacataa tggcacaaca tacgacacag aataaaacac
    11761 atgttttcaa ggaagaagaa aaaactgttt tgacttaatg tgaagcaggg gtttaccaaa
    11821 gaaaattgct ttaataagcc ctaactttaa ctgttttctt ctatgttaca gtgttagcgg
    11881 ttgatgtatt gtgtactgtt taagaactca taggagacta ataattaatt tactataatt
    11941 ttaaaggcaa agagttttat attaatttta ctgttgcaca agcagttatt ggaagtgggg
    12001 gaaaaaaaga aaactggtga gagaaattac atggaaatta taggaaaaag gaacttgaat
    12061 tggtaatttg gcaaagattt gggaacaaat agtttgaaca tagatttctt tcaccttata
    12121 tgatagaaac acaaggcaca cgtgagctgt atagtgtgct gagcacaccc ccagaaaaca
    12181 aaaaatatca cattgatgaa gtccgagact gtatctagat cttagcataa ctagtgaatt
    12241 cagggagcac atcctgctgc tgaaattgag aagtcagtaa gaaggtagtt cagatgacct
    12301 aatcattcaa gacatttgat ggagtccaca caagggttta tgatgtgggg caaatatctg
    12361 tgctttctca tctcagtgat tgatgtctgg agaactagtc agacacactt agctggttcc
    12421 tatagaaaaa caccagtaaa tgtggatcag accataccct gcagaccaag gagattttct
    12481 taaacttgct aagcctttct taaacttgcc aagtattagc aatgataatt gctaaatgga
    12541 ttttttcctt tcactgaaac aacaaaacaa ttattgaaat tcacaatacc tagagatggg
    12601 aacatggttt aggtttatcc agttctgtaa ctgactttgg cattggctca ttgtgtcctt
    12661 tctacaagtt atgttttggt gacttggtgt ccccagttag aaatggcaca caatattatc
    12721 agttgttagt acttggtaac gtttctttta ctaaaggaat tatataagta tgaatcatac
    12781 ctaattttac aatttctttc ccacggtcat tttggatata aagatagact tcccagacag
    12841 tgaggttaga aattagaagt tcaaattgat atccctgatg tttaatttaa aaatgaaagt
    12901 aaagttaaaa ttagtagcat ttgtaccaga gtagattatg ggtattttca tctgttatat
    12961 tacatgttca tattattcat tttaggatag gccaaaactc acttctaaaa tcttcaaaaa
    13021 ttttgaatag tttcattttt tcaatactta agccaaatga caattttata gatgtgaata
    13081 tatgtttttt aaagttaagt ccttcatatc ttttcttagt ttaactcccc aaagaggaaa
    13141 atgtctcttt aaaccagtag ttaaaaagat ggagtatgtg atgttccttt ctctgtactg
    13201 agttctgaat gtttaatcca gagcatggat cactgactgc gttctctgac tgtggaatgg
    13261 tattctttca tgcaaagtac tttcaaaact tcccataaag aaactaaact agagattgtc
    13321 tttattaatt aatggaactc tgggtatgat taagggaatt aaattagaaa gtagaataac
    13381 ctagttaaaa tcattatatt agttatacat gagagtcagt gaacgatgac ttgattttta
    13441 catgacattt gaattctgtc actatctgat tcttgaccta aaggtttctt tgctaaatct
    13501 gatatttatt atagtataat atgataattt ggagctcctg ttgcatctca ctgccccaaa
    13561 aatgttggaa tgccatatga tacagggaaa agattggagt tcatcaccgt ggaattttga
    13621 tctgtcttaa tgacacaact taattttcat gatgatagat ctctgaatga ttaatccaga
    13681 atgatttggt acagtgactg gaagaaattg tttttctggg aatttgcttc ccataatgac
    13741 ctgatataca ggtgacctta gtataattga aattaattat aattttaatt agaacatgct
    13801 ctgtttacag attataattg cgcagcattt taaaaaaata tacataatta tgaggatgag
    13861 agaattgcaa acttgaacag ttcttttgtt ttctctcctt ttacttgaaa agctttcaga
    13921 aagcttttga aagatgacac agttttcata gcaacagtat gcaaattagg tttttctgcc
    13981 aagaaactca taaactaaag tatggaataa cacaaatgct taatcttaat cttggctttg
    14041 gattttgtaa gcaaaagcat tttctgcatc aatttttttc agtatgcagt tgcatatttc
    14101 attttttgat tatgtcaaaa gttgaattct cattttatgc tgtgtttctc attagttgca
    14161 gtaaagtgtt gatttcattg ctgcctttca ggccttcatc atacaaaccc tcaccatttt
    14221 tcactcctgt gcttttgcca taggtttttt tttgtttttg gtttttttcc agcaactagt
    14281 ttttctactt tcactctttc ccaccagagt ggtctctcta aaacaaattt gctaaagttc
    14341 ttaagtaggc tcttttttct ctaaattaca gaatagcaaa ctaactccta agcaagatga
    14401 gtcagtgtct atgaccttgc ctctttcttc ctttttagta tcctcaccaa ccttccctct
    14461 gttgactttt gtaatccagg aatttggaac tacttatgac ttagtgcctt ggcataatca
    14521 catgccatag atgaacatgt tcaagactca gttcaaattt aactttctgt gaagccctac
    14581 ctgacttccc taggcaggta ttttctccat gcctatagta tattgaaact ttttctgtta
    14641 aaataacata acatttcaat tacacacaca caacctgtct cttccactga acttctctct
    14701 ccccctttgt attctcagtg tctagctaca cataatagga ctttgataaa tatttttgaa
    14761 tgaaggtaat agaaaacccg aacttctcta tatttgtgta ctttcacaaa caaaggctta
    14821 gaattccaca tgaaagtcca catgaggtaa gtgtgcattc atgagcatgt gtgcactctg
    14881 ggcaaaccaa cagaagataa acagcaagga agagcagttg ttttgtgaaa aataatcatc
    14941 tgttagctgt aaatcagaaa tattggccaa atcagaattc ttgtttctaa aatgaggctt
    15001 ctaaaatttt cacataactg ccaaactctt atttgtaatt cttaaatatg ccctaaagat
    15061 catctttctc ttttatagac atttagtgta tacagtccac ataaatacat atactgatta
    15121 cctacagaca ataatgagac ttcagaaaga atcaaaagca atagtgatgg tggaattttt
    15181 aaataatatt cagctggggg gaaatagact gagtttgtgt gatttcaggt tatttagagt
    15241 tattaatgta aacataagaa ggcttgtgac tcttatcacc ctctatagaa tataaagact
    15301 aaaaataaaa actattacct ctgggcctgt ttttctttgg gattagataa agcctttcta
    15361 ccatatcacc aaatactcat aaaggctaac acagaaagct tttccaagtt aataatttct
    15421 aaaattaaat atcagggcca ggtgtggtgg ctgacacctg taatcccagc actttgggag
    15481 gccgaagtgg gtggattacc tgaggtcagg agctcgagac aagtcaacat ggtgaaacct
    15541 catctctgct aaaaatacaa aaactagcca ggcatggtga tgcacacctg taatcccagc
    15601 tactcgggag gctgaggcag gagaattgct tgaacccagg aggcggaggt tgcagtgagc
    15661 tgagattgca ccactgcact ccagcctggg cgacagagtg agactctgtc tcaaaaaaaa
    15721 gaaaaaaaat caaaataatt gtaattaact gaaatgccct gttccttcat tcacttgata
    15781 gacttttcta cctgctctat acttggcact caattagatt ctggggatac agataaaata
    15841 agacaaagct cccatctggt agagtccaag tttgttttca cagttatttt tccacagtct
    15901 gtttaaggta ttaggaggac ttttataaac cgagtgtttt tcaggttact tcagagagga
    15961 gtctccttcc acccaaaaaa actaaccaaa ttattttatc ataaagaggt aatcccattt
    16021 ttatgtccac aatttttaca tttttgtatc agccaccaat aattttgact gttttgtcaa
    16081 gtacatattt catcaatcct atttccacag ccgtacatgt gatcccagtg actgatagat
    16141 ctgtagaatg tgaactcaca tttaaagttt attttgggaa ggcatatatg ccttttgatg
    16201 acacacacac caaaatatat aagagctgtg catggtaaac ccacagtaga agggaaaaag
    16261 taatgctttc tggtatactt cagaggctcc atttgaagat gtgcgttgat gtttccttga
    16321 ttattttgaa tgaggagtag gcggtaatat ttaacaggta gcatacgtat ctaagcatat
    16381 agtaaatatt actagtactt gtgtgccatg aggcactaat acataggtga aacaaaaata
    16441 tttttagttc taaaaaattc tctagatatt tatggatggt taggcaatag ggttccttca
    16501 tgagaaggaa aaaactgtat ctttattgtc agtaagtgtg gaaaaaaacc cgacttgtat
    16561 gtgtattcac agtttgatag gcatattttg aagtcattgt tttccatcag tattaagtat
    16621 catccatttc tatgttctct agcaaaagaa aattcttagt gtgatacagc tttactcttt
    16681 gtttgacttt gtacaaggtc cacaaaagga ggaggctcaa aagattggac actttaccct
    16741 gacattcagc acattcattt tatctttcga taaagataac cagaaatcat ttgcctcttg
    16801 tttattatca tgttgaaaac acaacagtat agttcctttt gtcccttaca ttggaaaagg
    16861 tattttaaat ttagccttgc cgtattattt atagttagga atttactaca ttttggtagt
    16921 ctgttttgaa actaagcact tgtgtctgaa agatgcagat aatatctatc tatttattac
    16981 acagtgggct ctgtttgctg cagaaaagag tgaaactttt aactgcttag cacacttgcc
    17041 agtgaaatat acctgacaga gcagaggtta taactttggc tctaattact tttagtaatg
    17101 gtaagacaac gtgagttttg tttgtttaca tgctgtttac aatggcataa ttttaaaaat
    17161 atatacaatt gagatattta ttgcctacta ttttaaagat gttttatgtg ttttcacctt
    17221 tggaatatat tgttacattt ccttgtacag ataattttgg tggctttgtt aatgtagcta
    17281 gtaattttta tgactactaa gtgaccagtt ttcggtgcct tttattgtgg gatgcatgat
    17341 tatgtattta ttgtacggaa gtcactgacg tgtgtatttt tgtactttgc tgaaagtaat
    17401 ggttgatcct gatgtacatg atgatgaaag acatctttcc caaagggcac tgccttgaga
    17461 tcatccttct caaataaaaa gaatgcattt gaagaaa
//
