LOCUS       NM_001346               5802 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant
            1, mRNA.
ACCESSION   NM_001346
VERSION     NM_001346.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Zhang,L., Xu,J., Zhou,S., Yao,F., Zhang,R., You,W., Dai,J., Yu,K.,
            Zhang,Y., Baheti,T., Pu,L., Xu,J., Qian,X., Zhang,C., Xia,Y.,
            Dai,X., Li,Q. and Wang,X.
  TITLE     Endothelial DGKG promotes tumor angiogenesis and immune evasion in
            hepatocellular carcinoma
  JOURNAL   J Hepatol 80 (1), 82-98 (2024)
   PUBMED   37838036
  REMARK    GeneRIF: Endothelial DGKG promotes tumor angiogenesis and immune
            evasion in hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Yamada,H., Mizuno,S., Honda,S., Takahashi,D. and Sakane,F.
  TITLE     Characterization of alpha-synuclein N-terminal domain as a novel
            cellular phosphatidic acid sensor
  JOURNAL   FEBS J 287 (11), 2212-2234 (2020)
   PUBMED   31722116
  REMARK    GeneRIF: Characterization of alpha-synuclein N-terminal domain as a
            novel cellular phosphatidic acid sensor.
REFERENCE   3  (bases 1 to 5802)
  AUTHORS   Guo,Z., Jia,J., Yao,M., Kang,J., Wang,Y., Yan,X., Zhang,L., Lv,Q.,
            Chen,X. and Lu,F.
  TITLE     Diacylglycerol kinase gamma predicts prognosis and functions as a
            tumor suppressor by negatively regulating glucose transporter 1 in
            hepatocellular carcinoma
  JOURNAL   Exp Cell Res 373 (1-2), 211-220 (2018)
   PUBMED   30399372
  REMARK    GeneRIF: Decreased expression of DGKgamma in hepatocellular
            carcinoma tissues was an independent risk factor for a poor
            prognosis.
REFERENCE   4  (bases 1 to 5802)
  AUTHORS   Kai,M., Yamamoto,E., Sato,A., Yamano,H.O., Niinuma,T., Kitajima,H.,
            Harada,T., Aoki,H., Maruyama,R., Toyota,M., Hatahira,T., Nakase,H.,
            Sugai,T., Yamashita,T., Toyota,M. and Suzuki,H.
  TITLE     Epigenetic silencing of diacylglycerol kinase gamma in colorectal
            cancer
  JOURNAL   Mol Carcinog 56 (7), 1743-1752 (2017)
   PUBMED   28218473
  REMARK    GeneRIF: Epigenetic silencing of DGKG expression is associated with
            skin cancer.
REFERENCE   5  (bases 1 to 5802)
  AUTHORS   Takeshita,E., Kume,A., Maeda,Y., Sakai,H. and Sakane,F.
  TITLE     Diacylglycerol kinase gamma is a novel anionic phospholipid binding
            protein with a selective binding preference
  JOURNAL   Biochem Biophys Res Commun 444 (4), 617-621 (2014)
   PUBMED   24486543
  REMARK    GeneRIF: these results indicate that DGKG is an anionic
            phospholipid binding protein that preferably interacts with a small
            highly charged head group that is very close to the glycerol or
            sphingosine backbone.
REFERENCE   6  (bases 1 to 5802)
  AUTHORS   Oyaizu,K., Kantarci,A., Maeda,H., Batista,E.L. Jr., Hasturk,H.,
            Murayama,Y., Badwey,J.A. and Van Dyke,T.E.
  TITLE     Identification of mRNAs for the various diacylglycerol kinase
            isoforms in neutrophils from patients with localized aggressive
            periodontitis
  JOURNAL   J Periodontal Res 38 (5), 488-495 (2003)
   PUBMED   12941073
REFERENCE   7  (bases 1 to 5802)
  AUTHORS   Yamada,K., Sakane,F., Imai,Si., Tsushima,S., Murakami,T. and
            Kanoh,H.
  TITLE     Regulatory role of diacylglycerol kinase gamma in macrophage
            differentiation of leukemia cells
  JOURNAL   Biochem Biophys Res Commun 305 (1), 101-107 (2003)
   PUBMED   12732202
  REMARK    GeneRIF: diacylglycerol kinase gamma regulates macrophage
            differentiation.
REFERENCE   8  (bases 1 to 5802)
  AUTHORS   Stohr,H., Klein,J., Gehrig,A., Koehler,M.R., Jurklies,B.,
            Kellner,U., Leo-Kottler,B., Schmid,M. and Weber,B.H.
  TITLE     Mapping and genomic characterization of the gene encoding
            diacylglycerol kinase gamma (DAGK3): assessment of its role in
            dominant optic atrophy (OPA1)
  JOURNAL   Hum Genet 104 (1), 99-105 (1999)
   PUBMED   10071200
REFERENCE   9  (bases 1 to 5802)
  AUTHORS   Yamada,K., Sakane,F., Matsushima,N. and Kanoh,H.
  TITLE     EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol
            kinase bind calcium with different affinities and conformational
            changes
  JOURNAL   Biochem J 321 (Pt 1) (Pt 1), 59-64 (1997)
   PUBMED   9003401
REFERENCE   10 (bases 1 to 5802)
  AUTHORS   Kai,M., Sakane,F., Imai,S., Wada,I. and Kanoh,H.
  TITLE     Molecular cloning of a diacylglycerol kinase isozyme predominantly
            expressed in human retina with a truncated and inactive enzyme
            expression in most other human cells
  JOURNAL   J Biol Chem 269 (28), 18492-18498 (1994)
   PUBMED   8034597
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117414.3, BC089411.1,
            D26135.1, AC112649.9 and BM669549.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001346.2.
            
            Summary: This gene encodes an enzyme that is a member of the type I
            subfamily of diacylglycerol kinases, which are involved in lipid
            metabolism. These enzymes generate phosphatidic acid by catalyzing
            the phosphorylation of diacylglycerol, a fundamental lipid second
            messenger that activates numerous proteins, including protein
            kinase C isoforms, Ras guanyl nucleotide-releasing proteins and
            some transient receptor potential channels. Diacylglycerol kinase
            gamma has been implicated in cell cycle regulation and in the
            negative regulation of macrophage differentiation in leukemia
            cells. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.150694.1,
                                           SRR1660809.188900.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265022.8/ ENSP00000265022.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-246               AC117414.3         134206-134451
            247-1886            BC089411.1         1-1640
            1887-3624           D26135.1           1866-3603
            3625-5202           AC112649.9         48694-50271         c
            5203-5802           BM669549.1         17-616              c
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.2-q27.3"
     gene            1..5802
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /note="diacylglycerol kinase gamma"
                     /db_xref="GeneID:1608"
                     /db_xref="HGNC:HGNC:2853"
                     /db_xref="MIM:601854"
     exon            1..289
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            290..604
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     CDS             538..2913
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1;
                     diglyceride kinase gamma; DAG kinase gamma; diacylglyerol
                     kinase gamma; diacylglycerol kinase, gamma 90kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase gamma isoform 1"
                     /protein_id="NP_001337.2"
                     /db_xref="CCDS:CCDS3274.1"
                     /db_xref="GeneID:1608"
                     /db_xref="HGNC:HGNC:2853"
                     /db_xref="MIM:601854"
                     /translation="MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQY
                     DPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANS
                     ADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVY
                     LKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDP
                     TELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTM
                     KHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEV
                     MQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTLCDGGELRDHI
                     LLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGER
                     ILRKFHYLLNPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANF
                     AKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPRE
                     EVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSET
                     FAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRE
                     SRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRT
                     NKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD"
     exon            605..681
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            682..847
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            848..910
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1081
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1131
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1206
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1329
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1447
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1536
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1653
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1746
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1806
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1886
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1961
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2047
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2137
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2138..2298
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2363
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2454
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2632
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2753
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2814
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..5802
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5480..5485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /note="hexamer: AGTAAA"
     polyA_site      5503
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
     regulatory      5781..5786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /note="hexamer: AATAAA"
     polyA_site      5802
                     /gene="DGKG"
                     /gene_synonym="DAGK3; DGK-GAMMA"
                     /note="major polyA site"
ORIGIN      
        1 gcagtgccgt agtagcgggc cacggagata gacagctttg gagctgctga actccgagca
       61 cagggtgaag accccggcgc taccaaccac agcctggcag cctggtctcc gcggcaccca
      121 ctggggctgc atccccctcc cccgagaggg ctgcgcaggc gggaagacgc ccagaggcca
      181 gcttcggtcc cccttctgtc tctcggttcc tctttcctcc caagtaaggg aataaaccgc
      241 gaagaaggag cgccccgggc caccgcgcaa ccaagtgttg cctggtgagg aagagccagg
      301 acttctgaat ttaccttgaa tacagacagg aggatgttgc ctaaggaata gcagagatct
      361 tgtctcatct tctgagaggt gcctgctgct gctgtataca cttgagtgct cccagaagtc
      421 tcctgaaagg cttacatcgc aaacctgcaa tgagccaggc cctgggctgg gcctccactt
      481 cagcctagtg aacaaaactc catcactgcc ctttagccac tcacataaag tttaaaaatg
      541 ggtgaagaac ggtgggtctc cctcactcca gaagaatttg accaactcca gaaatattca
      601 gaatattcct ccaagaagat aaaagatgcc ttgactgaat ttaatgaggg tgggagcctc
      661 aaacaatatg acccacatga gccgattagc tatgatgtct tcaagctgtt catgagggcg
      721 tacctggagg tggaccttcc ccagccactg agcactcacc tcttcctggc cttcagccag
      781 aagcccagac acgagacctc tgaccacccg acggagggag ccagcaacag tgaggccaac
      841 agcgcagata ctaatataca gaatgcagat aatgccacca aagcagacga ggcctgtgcc
      901 cctgatactg aatcaaatat ggctgagaag caagcaccag ctgaagacca agtggctgcg
      961 acccccctgg aaccccccgt ccctcggtct tcaagctcgg aatccccagt ggtatacctg
     1021 aaggatgttg tgtgctacct gtccctgctg gagacgggga ggcctcagga taagctggag
     1081 ttcatgtttc gcctctatga ttcagatgag aacggtctcc tggaccaagc ggagatggat
     1141 tgcattgtca accaaatgct gcatattgcc cagtacctgg agtgggatcc cacagagctg
     1201 aggcctatat tgaaggagat gctgcaaggg atggactacg accgggacgg ctttgtgtct
     1261 ctacaggaat gggtccatgg agggatgacc accatcccat tgctggtcct cctggggatg
     1321 gatgactctg gctccaaggg ggatgggcgg cacgcctgga ccatgaagca cttcaagaaa
     1381 ccaacctact gcaacttctg ccatatcatg ctcatgggcg tccgcaagca aggcctgtgc
     1441 tgcacttact gtaaatacac tgtccacgaa cgctgtgtgt ccagaaacat tcctggttgt
     1501 gtcaaaacgt actcaaaagc caaaaggagt ggtgaggtga tgcagcacgc atgggtggaa
     1561 gggaactcct ccgtcaagtg tgaccggtgc cacaaaagta tcaagtgcta ccagagtgtc
     1621 accgcgcggc actgcgtgtg gtgccggatg acgtttcacc gcaaatgtga attatcaacg
     1681 ttgtgtgacg gtggggaact cagagaccac atcttactgc ccacctccat atgccccatc
     1741 acccgggaca ggccaggtga gaagtctgat ggctgcgtgt ccgccaaggg cgaacttgtc
     1801 atgcagtata agatcatccc caccccgggt acccaccccc tgctggtctt ggtgaacccc
     1861 aagagtggag ggagacaagg agaaagaatt cttcggaaat tccactatct gctcaacccc
     1921 aaacaagttt tcaacctgga caatgggggg cctactccag ggttgaactt tttccgtgat
     1981 actccagact tccgtgtttt ggcctgtggt ggagatggga cagttggctg gattttggat
     2041 tgcattgata aggccaactt tgcaaagcat ccaccagtgg ctgtcctgcc tcttggaaca
     2101 ggaaatgacc ttgcccgttg tctccgctgg ggaggaggtt atgaaggggg cagcttgaca
     2161 aaaatcctga aagacattga gcagagcccc ttggtgatgc tggaccgctg gcatctggaa
     2221 gtcatcccca gagaggaagt ggaaaacggg gaccaggtcc catacagcat catgaacaac
     2281 tatttctcca ttggtgtgga cgcttccatt gcacacagat tccatgtgat gagagagaaa
     2341 catcctgaaa aattcaacag caggatgaag aacaagctgt ggtactttga atttggcacc
     2401 tcggagactt ttgcagcgac ctgcaagaaa ctccacgacc acattgagtt ggagtgtgat
     2461 ggggttgggg tggacctgag caacatcttc ctggaaggca ttgccattct caacattccc
     2521 agcatgtacg gaggcaccaa tctctgggga gaaaacaaga agaaccgggc tgtgatccgg
     2581 gaaagcagga agggtgtcac tgaccccaaa gaactgaaat tctgcgttca agacctcagt
     2641 gaccagctcc ttgaagtggt ggggctagaa ggagccatgg agatggggca gatctacacc
     2701 ggcctgaaga gtgcaggcag gaggctggcc cagtgcgcct ctgtcaccat caggacaaac
     2761 aagctgctgc caatgcaagt ggatggagaa ccctggatgc agccatgttg cacgattaaa
     2821 attactcaca agaaccaagc gcccatgatg atggggcctc cccagaagag cagcttcttc
     2881 tcgttgagaa ggaagagccg ttcaaaagac taaacagtgt gccaaacacc agctaaacca
     2941 agagagaaag caagaaacta taatgcacac tcacacacaa tttatgtgca cactcacaca
     3001 tgcacacaca cacacacata cacactcttc tctaaccagt ggaagcaaag ccacccttcg
     3061 ggaagaaaac gtcaccttgc catacattct gtttcaacag tgggtacacc cctaacagag
     3121 ccagtgccaa caaaacattt tgaatggact tagggcccat gaggttgtgg ctggcttagg
     3181 cagcaacctc cacattccca caggccttga gcagaatttt ctgagactga agggaaatcc
     3241 ccctttcttt ctaccagccc tgcaagtttc ctcatggacg ctcgcgagga gcaggctgca
     3301 ggtttcctgc ctatggtgag atcagatgtg gccaagggaa ggagctctgg ttccagagaa
     3361 tttgcacaaa gttccctctg tacagagaca aaacggcctc cggctctcag agcataatcc
     3421 ttggcagggc tcagcaggcg cacgttggtt tcttggtcgt cctttgagtg acaacttctc
     3481 cgtgaacctg ctgaagaggc agaaaggctg tggaaagctg tatttccatt cttgggtttc
     3541 tgcgccgtcg gtgggcactt gttattttcc aggaaccttc tcctggtgtc tacatgtttg
     3601 cttagaggcg gctccaagag cccccagagc tgcctgcata gcacacctta gatgtggtat
     3661 ttattttctt agttctgtga acacctggga gggagagcgg agaaactggg atttattttt
     3721 caaattggtg tcataatatt gtgtaaaaag ggaaggaaaa aaaaaaccac ccccagcttc
     3781 tttctttttc aagccaaagc gtgtttcctg tttgagttcc aagccctggt ggcagactat
     3841 tcctgaaggc agaaaagatg ggaaggaagg aactttgctg gggaaaccag gagacggttc
     3901 cctcctgctt ctttgcactc accactccca gagtatgacc tgaaggacct tctgatggtc
     3961 ttaacgtgtg tgcgcgcgtg tgtgtgtgtg tgtgcgtgta tatatatata tttagcctat
     4021 atatttatat atatatatat ataaatattt atttactgat ggacttttcc tcattggaaa
     4081 aaaaaaacat aggtagtatg agacagaaag tggtaaggaa aaagaaggtt ctcactcccc
     4141 cactccaccg tttcattgtc aggacgcatc tccctgtggt gatcacaagg gttaagggga
     4201 atgaaagtag ctccctatta tgaacgaaga gaccgagaac tcctattctg gatcctcaaa
     4261 acaagcatga aagacctgct gggctggatt tactctccca tttgtcagat aagaaaattg
     4321 agtctcagaa aagttcagag acttttccat ggtgtcttgg ctaatgcagc ttgagagtag
     4381 gtgccgttac aaagatattt aatggagaaa agaacccaca catctcagag tagggtcatt
     4441 tctggtcact gaaaagtgag tccacttgga tccaggttgc cagggaggaa caaacgtaca
     4501 gcacatgcgt ggatgggata tccccacaca agaaaacgag cgcctcatgc tgcttcccag
     4561 aggttctctc tgacctgaat ggaaaagaag ttggtctgag atcgaaggta ctaaacagat
     4621 catcagactg aactctccac atgtcacctc atcatggtca tcatagtctc ttagtttgga
     4681 aacaaccttg gctgcaggga tgacctcagt ggagagtcac aagaaggact gaggcgtctt
     4741 ctgcaagata ctgccagcca ggacagaggg gtctcagcag agctcagtgg gcttcattca
     4801 gcaagtgcca gcattaccat cctttcccaa gggcaaggcc atgcagagct tcattgggcc
     4861 tccatccctc tctgagattt atcagtggaa acttggaccc acttgatatc gttccactga
     4921 gctgcctcta atcttgcatt ggacaggagc tttggacaaa gaagggaata ttaagattgt
     4981 gtgtacaaat ggcacttaac tgtgaaggac cacctaggag caagatcttg gaccattctg
     5041 ctcaacctaa cagtgcagaa tgctaagtaa ggggaaaacc aggccacctg aagaatatct
     5101 ttaatgatgg tattcttggt gatgggacca acaagctcca gaattactac aggtactatc
     5161 ttggaagcca cttgtttctg aaactgaggc aggttcaggt ttggggtcac tgattctaag
     5221 acatttacag aagttcgtgc atgtgtttca gccatgatcc ccaagattga ggctattgag
     5281 acatggcact atcaacaggt gaaattgatt ctcagagact cacaggtctc atgaaggggc
     5341 agtgcagaaa atggccctta cctgctgcac cctagtgcac aggacttgca atgttgcttg
     5401 agagacatat cccaagtcct tcttagaggc atggagtata acaacttcag tggaattgca
     5461 cttccagttt ggtaacctta gtaaatcttg tgttgttgtt taatcgtgcc ttgcaaatca
     5521 tcctttaaac actatgactg tggtttcttg tggtggagtc cctcatcata gtgaccctca
     5581 tagcaacctc tgaggttagc agagaaggta ttattatctc gatctcaatg aggagaacaa
     5641 ggcttaatga ggtttagtga cttctcaaag ccacatggtt agtcagggga gaacctaatc
     5701 ccttatttct tatcttattc tgattctggc catgctacaa acatgttctc acctgccccc
     5761 catgcttcta ccccaacagc aataaaaact gtaaagcagg aa
//
