LOCUS       NM_003647               8608 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens diacylglycerol kinase epsilon (DGKE), mRNA.
ACCESSION   NM_003647
VERSION     NM_003647.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Traczyk,G., Hromada-Judycka,A., Swiatkowska,A., Wisniewska,J.,
            Ciesielska,A. and Kwiatkowska,K.
  TITLE     Diacylglycerol kinase-epsilon is S-palmitoylated on cysteine in the
            cytoplasmic end of its N-terminal transmembrane fragment
  JOURNAL   J Lipid Res 65 (1), 100480 (2024)
   PUBMED   38008259
  REMARK    GeneRIF: Diacylglycerol kinase-epsilon is S-palmitoylated on
            cysteine in the cytoplasmic end of its N-terminal transmembrane
            fragment.
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Traczyk,G., Swiatkowska,A., Hromada-Judycka,A., Janikiewicz,J. and
            Kwiatkowska,K.
  TITLE     An intact zinc finger motif of the C1B domain is critical for
            stability and activity of diacylglycerol kinase-epsilon
  JOURNAL   Int J Biochem Cell Biol 152, 106295 (2022)
   PUBMED   36113832
  REMARK    GeneRIF: An intact zinc finger motif of the C1B domain is critical
            for stability and activity of diacylglycerol kinase-epsilon.
REFERENCE   3  (bases 1 to 8608)
  AUTHORS   Liu,D., Ding,Q., Dai,D.F., Padhy,B., Nayak,M.K., Li,C., Purvis,M.,
            Jin,H., Shu,C., Chauhan,A.K., Huang,C.L. and Attanasio,M.
  TITLE     Loss of diacylglycerol kinase epsilon causes thrombotic
            microangiopathy by impairing endothelial VEGFA signaling
  JOURNAL   JCI Insight 6 (9), 146959 (2021)
   PUBMED   33986189
  REMARK    GeneRIF: Loss of diacylglycerol kinase epsilon causes thrombotic
            microangiopathy by impairing endothelial VEGFA signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8608)
  AUTHORS   Bezdicka,M., Pavlicek,P., Blahova,K., Hacek,J. and Zieg,J.
  TITLE     Various phenotypes of disease associated with mutated DGKE gene
  JOURNAL   Eur J Med Genet 63 (8), 103953 (2020)
   PUBMED   32413569
  REMARK    GeneRIF: Various phenotypes of disease associated with mutated DGKE
            gene.
REFERENCE   5  (bases 1 to 8608)
  AUTHORS   Epand,R.M., Kam,A., Bridgelal,N., Saiga,A. and Topham,M.K.
  TITLE     The alpha isoform of diacylglycerol kinase exhibits arachidonoyl
            specificity with alkylacylglycerol
  JOURNAL   Biochemistry 43 (46), 14778-14783 (2004)
   PUBMED   15544348
REFERENCE   6  (bases 1 to 8608)
  AUTHORS   Tang,W., Bardien,S., Bhattacharya,S.S. and Prescott,S.M.
  TITLE     Characterization of the human diacylglycerol kinase epsilon gene
            and its assessment as a candidate for inherited retinitis
            pigmentosa
  JOURNAL   Gene 239 (1), 185-192 (1999)
   PUBMED   10571048
REFERENCE   7  (bases 1 to 8608)
  AUTHORS   Hart,T.C., Price,J.A., Bobby,P.L., Pettenati,M.J., Shashi,V., Von
            Kap Herr,C. and Van Dyke,T.E.
  TITLE     Cytogenetic assignment and physical mapping of the human DGKE gene
            to chromosome 17q22
  JOURNAL   Genomics 56 (2), 233-235 (1999)
   PUBMED   10051413
REFERENCE   8  (bases 1 to 8608)
  AUTHORS   Tang,W., Bunting,M., Zimmerman,G.A., McIntyre,T.M. and
            Prescott,S.M.
  TITLE     Molecular cloning of a novel human diacylglycerol kinase highly
            selective for arachidonate-containing substrates
  JOURNAL   J Biol Chem 271 (17), 10237-10241 (1996)
   PUBMED   8626589
REFERENCE   9  (bases 1 to 8608)
  AUTHORS   Martin,B. and Smith,R.J.H.
  TITLE     C3 Glomerulopathy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301598
REFERENCE   10 (bases 1 to 8608)
  AUTHORS   Noris,M., Bresin,E., Mele,C. and Remuzzi,G.
  TITLE     Genetic Atypical Hemolytic-Uremic Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301541
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL555194.3, DB221284.1,
            BC130629.1, AC015912.16 and BM682264.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003647.2.
            
            Summary: Diacylglycerol kinases are thought to be involved mainly
            in the regeneration of phosphatidylinositol (PI) from
            diacylglycerol in the PI-cycle during cell signal transduction.
            When expressed in mammalian cells, DGK-epsilon shows specificity
            for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed
            predominantly in testis. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.135353.1,
                                           SRR1803616.12376.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284061.8/ ENSP00000284061.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-364               AL555194.3         53-416
            365-443             DB221284.1         78-156
            444-707             DB221284.1         158-421
            708-2070            BC130629.1         604-1966
            2071-7350           AC015912.16        50296-55575
            7351-7716           BM682264.1         17-382              c
            7717-8608           AC015912.16        55942-56833
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q22"
     gene            1..8608
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="diacylglycerol kinase epsilon"
                     /db_xref="GeneID:8526"
                     /db_xref="HGNC:HGNC:2852"
                     /db_xref="MIM:601440"
     exon            1..110
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            111..592
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..1832
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /EC_number="2.7.1.107"
                     /note="DGK-epsilon; DAG kinase epsilon; diglyceride kinase
                     epsilon; diacylglycerol kinase, epsilon 64kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase epsilon"
                     /protein_id="NP_003638.1"
                     /db_xref="CCDS:CCDS11590.1"
                     /db_xref="GeneID:8526"
                     /db_xref="HGNC:HGNC:2852"
                     /db_xref="MIM:601440"
                     /translation="MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQR
                     SRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKA
                     DKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQ
                     KTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWT
                     PLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPIKALQLCTLLPYYSARVLV
                     CGGDGTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQ
                     VLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREK
                     APSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIG
                     YWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVR
                     LILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQEDIKAT
                     E"
     exon            593..752
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            753..872
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1016
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1174
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1226
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1340
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1412
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1540
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1652
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..8608
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2377..2382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="hexamer: AGTAAA"
     polyA_site      2400
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="major polyA site"
     regulatory      7691..7696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="hexamer: AATAAA"
     regulatory      7697..7702
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="hexamer: ATTAAA"
     polyA_site      7711
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="major polyA site"
     polyA_site      7716
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="major polyA site"
     regulatory      8589..8594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
                     /note="hexamer: AATAAA"
     polyA_site      8608
                     /gene="DGKE"
                     /gene_synonym="AHUS7; DAGK5; DAGK6; DGK; NPHS7"
ORIGIN      
        1 aagcgtttcc cccgcccggc ttcatccctg ctggcggccc agcgtcgttc tcctcctgcg
       61 cgaggcggcc aaggcctgct ggcccggagc cgcgcctcca cccgcgcgag gtatcgtcct
      121 tggagaagat ggaagcggag aggcggccgg cgccgggctc gccctccgag ggcctgtttg
      181 cggacgggca cctgatcttg tggacgctgt gctcggtcct gctgccggtg ttcatcacct
      241 tctggtgtag cctccagcgg tcgcgccggc agctgcaccg cagggacatc ttccgcaaga
      301 gcaagcacgg gtggcgcgac acggacctgt tcagccagcc cacctactgc tgcgtgtgcg
      361 cgcagcacat tctgcagggc gccttctgcg actgctgcgg gctccgcgtg gacgagggct
      421 gcctcaggaa ggccgacaag cgcttccagt gcaaggagat tatgctcaag aatgacacca
      481 aggtcctgga cgccatgccc caccactgga tccggggcaa cgtgcccctg tgcagttact
      541 gtatggtttg caagcagcag tgtggctgtc aacccaagct ttgcgattac aggtgcattt
      601 ggtgccagaa aacagtacat gatgagtgca tgaaaaatag tttaaagaat gaaaaatgtg
      661 attttggaga attcaaaaac ctaatcattc caccaagtta tttaacatcc attaatcaga
      721 tgcgtaaaga caaaaaaaca gattatgaag tgctagcctc taagcttgga aagcagtgga
      781 ccccattaat aatcctggcc aactctcgta gtggaactaa tatgggagaa ggactgttgg
      841 gagaatttag gatcttgttg aatccagtcc aggtttttga tgtaactaaa actcctccta
      901 tcaaagccct acaactctgt actcttctcc catattattc agctcgagta cttgtttgtg
      961 gaggggatgg gactgtaggg tgggtcctgg atgcagttga tgacatgaag attaagggac
     1021 aagaaaagta cattccacaa gttgcagttt tgcctctggg aacaggcaac gatctatcca
     1081 atacattggg ttggggtaca ggttatgctg gagaaattcc agttgcgcag gttttgcgaa
     1141 atgtaatgga agcagatgga attaaactag atcgatggaa agttcaagta acaaataaag
     1201 gatactacaa cttaagaaaa cccaaggaat tcacaatgaa caactatttt tctgttggac
     1261 ctgatgctct catggctctc aattttcatg ctcatcgtga gaaggcacca tctctgtttt
     1321 ctagcagaat tcttaataag gcggtttact tattctatgg aaccaaagat tgtttagtgc
     1381 aagaatgtaa agatttgaat aaaaaagttg agctagaact ggatggtgag cgagtagcac
     1441 tgcccagctt ggaaggtatt atagttctga acatcggata ctggggcggt ggctgcagac
     1501 tatgggaagg gatgggggac gagacttacc ctctagccag gcatgacgat ggtctgctgg
     1561 aagtcgttgg agtatatggg tctttccact gtgctcagat tcaagtaaaa ctggctaatc
     1621 cttttcgaat aggacaggca catacagtga ggctgatttt gaagtgctcc atgatgccaa
     1681 tgcaggtgga tggggagcct tgggcccaag ggccctgcac tgtcaccata actcacaaga
     1741 cacatgcaat gatgttatat ttctctggag aacaaacaga tgatgacatc tctagtactt
     1801 cggatcaaga agatataaag gcgactgaat agatggatga gggagtgaaa actttgcata
     1861 gaatcctcac gcaagtagat acatgttcat ccaaaagtat taatagaaat tctctatcag
     1921 ctattcagtc ttaatttcac tagtagtata atgggtatac atttttgtaa atagcatccc
     1981 caaaccagcc agccttcagt tatttacaaa tgtttgttct tttttcagca aaatacttca
     2041 aatgaatagt attaacttac aaaaagtcac aaaaacttac atgagagtga aaatttgtta
     2101 tgactgtttt gagagtggga ctcactctga agtatgtgct gtctcatgtc ttatttttga
     2161 accatgcata tgatggacac acaatggatg gacacattat atctccaaca aggtgtgggt
     2221 ggaaagatca aattaacctg cttttttgaa aggaaatgat tactgtcaaa ccagcatggt
     2281 taattgtgag catcctcctg cagcatgccc cttaagattt tctacaaccc aaaccaagtg
     2341 tatgtattga tttctaggaa cccccaaaag gagaatagta aaaaaagatc atacttaaaa
     2401 tttgtattac aatttttatt ttaggaactt attcagacac gtaaatgttg tttaattctg
     2461 taggtaacca tttgagctgc aattcaggat cttttttata acaccagtgt agccaaaaga
     2521 gaaacagata agtgaattgg taagaaataa gattcagagc acttgggatt gtaagttata
     2581 ggttctgagc tgaactgttt atcagccgct ctttttatta aaagacagtg tcaacacttc
     2641 aaaaaacgtg cttaaaggag acaacggtga gattaattgc ttagaaaaag ctcaaggctg
     2701 actgataagt tttaaaaatt tggtggtctt gtagtatttt gaaatacagt agtcttttaa
     2761 cagaatttcc ccttagggag gtaagtggac agaaatcttt gctaaaatgt ttatatcata
     2821 tttattttaa aatgagagag atatattttt aaattattta ttatttacct tgtggtatgg
     2881 tacctgtgtt tagtgtttac gttctgtagc tttatttaat aagttaaatt gatcacataa
     2941 attctgctag tcacaatcag atagaaattt agtctattaa ttaaaattct aatttaattc
     3001 tcctgacaga aatctagata ttcttattct tcacaaatat tcctcattgt attgaaaaat
     3061 gaaggagggc atgaaaagca ctagggaact acttttggat aactgaaagc tttgtttcat
     3121 tgttttattt gcccctcaat tgccacaaac ttactgaagg gctagtttca tggttcccat
     3181 tagagggtgc ttgttcgctg ctgttgacca gcccaagggt cgtgacccac aggtcggctg
     3241 acccagaggg catccagcct ggtgtttttc tgacacatgg cccaggcttt gtggtctttc
     3301 atgacattaa tctctgaaag atcagaatgt atcccccaaa ataccattag ctatctttga
     3361 gggtctatta tgaatctgtc atccatgaat tgccataaac ctaagtttgc ctcacctcag
     3421 gcccagacca tgttttcaaa gagcaactta gtaatgtata cagtagtcag tacattttat
     3481 gaaatactct attttgctaa attaacctct tgcagatctc ctcaggtgat cttaaattct
     3541 agtcttgaga aactttgtga gcagatctgt tttcagcttc tttatatacc tttcatggta
     3601 agtaaccatg gcagtgagga tggcagagtt ttgttttttt tttttttaat gggccaatca
     3661 tgttctgagt attatgaagt ttatcctttc attcatgatc aaaattgtta aagacatcac
     3721 attttataaa tacactgtta aatccacttt gctgtaggtt atagtttacc tgttatataa
     3781 ttcgtagtgc taattgtagt agaagcaaat caatttagga tgttggaacg aaaataatca
     3841 caataactaa tacagctttt taattgtgaa aactacaatt aaaaattata attttaatac
     3901 caaccataat attacactgt atttagaaca ggcttatgta aaatgccctc atttatctaa
     3961 ataagttgat tttttggtta ttgtaatcta gtaaggtttt tgcatcacta ctggtctgtc
     4021 atgaagtttt tctgctggct tattcactta tttagacttc atttagggaa gctatttcag
     4081 aataccatct cataaaatgt atcattatct tgtttttgca aaattcactt actttttggg
     4141 cacatttacc cagcatttca aataagtgta taggcatgtg ccttgtttaa gtacaattta
     4201 taagacttca tgtttgcaag ttacatatta gggtagtgat tgtttatatt accagggatt
     4261 ctacacttta ccaaaaaaaa ttgccataag attttcctgt tccattttaa attgtcttga
     4321 tatttaaaaa aataacttct acattctcag gaagaaatac atgaagtaaa tcatatctcc
     4381 atgtctgttt ccagatttct ctaatctttt tactatccca atacattcct aattcctata
     4441 aacgtagtgg cttaaatact gaatacctgg cagctcttaa agatccctta ggcagatgat
     4501 gataattttc agtgtttcgt catgtttaat agaaatcaaa ctgacttgtg ttttaaaata
     4561 caagttgtct gctagtaaag ttacatgatt taggtgtgtt ttaggttttc tgcaacaatt
     4621 agctgtgaac ccctaatgta gctaagttta tacaaatttt ataatcaaaa ttatttattt
     4681 ttattttttt agagtatgtc aaaaacatat agtgtttgct ttgtgaaata catttgctta
     4741 gagttataaa atatactgtg actcagaaat tgtaaagtca gtttgcgttg taaaaatgaa
     4801 tactaccagc atgtcaaaag aaaaataatt tacatttgta atataaagta aaattgtatt
     4861 taatatattt attgaatttt aaatttgcac taacttgaaa actttaaagg ttatactaat
     4921 ttaatgatta atgtggaccc aaatacataa tttggccaaa aaagtcatta gaatgggtaa
     4981 tggttttgtg tggttttcct tcaaaatgat aatattgaag caagaaccag cattttatat
     5041 tgataatttt tttaatattt ctacccagtt gaggacagtg gaatataagt agatatattt
     5101 aaaatatcta gatatttaaa aagaaagaaa aggttgttta tcccaatctg aattcattct
     5161 cagcaacaag acagaaaagg tggtttgggt tttgctttta aacagtgtcc ccctgtcttt
     5221 ttcaattttt cccttgaagc ctttctcttg ttcttcagag tacgcgctgc aggctatctg
     5281 cagcatcaga atgcctgggc aggctgttga aagtatggat tcctgagccc aacccttaac
     5341 ttaatgattc cctgtctctg gagatgcaac atagaaatct ctgtttttat taacaagcgc
     5401 ctcaggcgat tcttaagcat actgaaattt gaaagcttct ggtctgaacc tcatgtattc
     5461 acctagggct tttgctaggt gggataccat gccgaaaaat tgaattaatt gtatgttctt
     5521 aaaattgttt ttcttctact ccgctgtcct cttccctcaa aatcactttt ctaaaagaac
     5581 tctatgaaaa acttatttgt atcataccca aattgatata tttgtctgaa ctctgttaca
     5641 taaaaattgg tatgattcat atgtggaatc agtttttgga ctgtgaccca tgcagtgttg
     5701 ggggacaagg agttgcttca ggagttagtg cagtgcgcct ccaaggggca gtgagccatg
     5761 gaggcgcctg ccttctcact gcagtcaatg cagccacact tgtgtatact gagtgctgct
     5821 tcagatctca gtagataaaa taattccaca tttaaaatga ttctgctatt ttttataaag
     5881 ggtttgaaag ccactactat aatgactttg tcacctaatt gattttctac taatttttac
     5941 atattcagaa acttcgtgtt tttttggtgg acagaatatt ttggaggtct ccgtcgctgt
     6001 ttagaatgtg gccacttggc tgtttcacag tttttaaagc tattttattg aatagtaaag
     6061 cagtgaactt agcctttgtt ctttgtgttt ctaatttaag catatagtct atttaatttt
     6121 tgatatgtaa atacacatca atacatatat atttacccat gtggccctgt gtagtcagtg
     6181 catgaggctt gcatagccca gaaaggtaca ttccagggtt ctggggaaag aattttaaaa
     6241 tgccatcctc taatacagac gtttataaaa cttaaatgaa atgattgggc ttaaccatat
     6301 gcaagaaagt ctgcagaaaa taaatcacct agaaactata aatagaaatg tgctgctgag
     6361 gctgggcacg gtggctcaca cctgtaatcc cagcacttgg ggggctaagg gaggcgtatc
     6421 acctgaggtc aggagtttga gacaagcctg gtcaacatgg tgaaacgccg tctctactaa
     6481 aaatacaaaa aaaattagcc aggtacattg gcacatgcct ttaatcccag ctaatcagga
     6541 ggctgaggca cgagaattgc tcgaacccag gcggcagagc ttgcagtgag cggagatcac
     6601 atgactgcac tccagcctgg gcaacagagc aagactccat ctcaaaaaaa aaaaggccgg
     6661 gcgtggtggc tcacgcctgt aatcccagca ctttgggagg ctgaggcagg tggatcacga
     6721 ggtcaggaga tcgagactat cctggctaac acggtgacac cccgcctcta ctaaaaatcc
     6781 aaaaatttag ccgggcatgg tggcgggcac ctgtagtccc agctactcgg gaggctgagg
     6841 caggagaatg gcgtgaaccc gggaggcaga gcttgcaggg aggcggagct tgcagcgagc
     6901 cgagatagtg ccactgcagt ggggcctggg ccaaagagtg agactccatc tcaaaaaaaa
     6961 aaaaaaagaa agaaatgtgt tgctggtatc agatatcaga ccaagaacac ttcagtctct
     7021 ctaaggatgc cctgagctac ctcactgtta aaggacgaca tcaacacaga atgcactaaa
     7081 caggaaataa gctgtaatct agagaatttc cattatgtgt tactttttgg tgactaacat
     7141 ggaatgttga aaaggaagag ctggaaagct cagttgtttt ccttgttcct ctgacattgt
     7201 ccaggcaaga gggcatcctg atcagatgag tagatttggc tgagaaaaac cctagagtaa
     7261 ggcaggcact ttgtggaggt ggatgatgat ggctcataaa aacgtttgtt ctcagtccag
     7321 ttcagggctc tgccagcagt ctttcagatc tgaactgctt aaacaaaccc tacagataaa
     7381 ttggcactct gatttgtaat tctgtttgta caagtttaga gcagcctagc tcgagtcctc
     7441 aaccccagtc ctcttagaag tgaactgatt gcactggatc cctaaaccca caatgttgag
     7501 gacacatgtg atgactccac ttgctcagcc agctggcctc ttgcactttc ccctgcccac
     7561 cacttgtaac taccacttaa ttatcttgtg ttaattgctt ttgttgtgtt gggtctgtat
     7621 ttttgtggtc agtgcctgca ggcagaaatg tgaaagcatt tggtatgttg aagatacttg
     7681 cttctttttt aataaaatta aaagtgcagc acgtaagtat gatactgtgt agttttttga
     7741 cacaaccatg agatacaata agcagctttg acttagtgtc ccaaaaagtg gttcttggtc
     7801 tacagcaggg caaacatata tgtggcaagt tctgatcaca tacttttaga cagaaagaat
     7861 aaaaaattca tattgcatgg cttttgtagc ctaagagcac agaatcatac acgtgtgtta
     7921 ggagaaacat tcattctcac gcatataaac tggctcctgg cagagtagag cagtaagtgg
     7981 gatcaaaggt gaattcacct tattttcagt tggtagagta tggaaaaatg tatcacttat
     8041 ttgaaatacc tgaatggaaa cccagcctct actactgtaa cttaacactt gttcttcctg
     8101 agcctcaaat tttctttctc tgtaagaatg ggaattaatg cccacctacg ggttgcaagt
     8161 gcttacagga gctgggcaag caacgaaggt aagagttgta gagacttcgg taaactggag
     8221 cacatgattc ctgggaagca ggcctagtgt aaacaattta tttttctaga aaagacagaa
     8281 gtttagagta tatgaaatct aatttttaag tattggttgg caactaattg actatcgtct
     8341 accataaggt tatatgataa ttattagggc aggagagtga atgcatctta atatgcatgg
     8401 cagaactgtg tgtttccttc catctggatt ttcataaagc tttctgattt atcagtaacg
     8461 atctgaaaaa tgtactgtgg catgtaacat cttttattca ttttattagg cattagagga
     8521 agaatattct gtagtcctgc tttattctgc catctttacc tggaaatcca tttttataaa
     8581 atttttgtaa taaaaattca cttgatca
//
