LOCUS       NM_152879               6308 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant
            2, mRNA.
ACCESSION   NM_152879
VERSION     NM_152879.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Murakami,C., Hoshino,F., Sakai,H., Hayashi,Y., Yamashita,A. and
            Sakane,F.
  TITLE     Diacylglycerol kinase delta and sphingomyelin synthase-related
            protein functionally interact via their sterile alpha motif domains
  JOURNAL   J Biol Chem 295 (10), 2932-2947 (2020)
   PUBMED   31980461
  REMARK    GeneRIF: Diacylglycerol kinase delta and sphingomyelin
            synthase-related protein functionally interact via their sterile
            alpha motif domains.
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Jennings,W., Doshi,S., Hota,P.K., Prodeus,A., Black,S. and
            Epand,R.M.
  TITLE     Expression, Purification, and Properties of a Human
            Arachidonoyl-Specific Isoform of Diacylglycerol Kinase
  JOURNAL   Biochemistry 56 (9), 1337-1347 (2017)
   PUBMED   28199087
  REMARK    GeneRIF: purification and characterization of DGKepsilon's
            enzymatic and conformational properties
REFERENCE   3  (bases 1 to 6308)
  AUTHORS   Blandin,G., Marchand,S., Charton,K., Daniele,N., Gicquel,E.,
            Boucheteil,J.B., Bentaib,A., Barrault,L., Stockholm,D., Bartoli,M.
            and Richard,I.
  TITLE     A human skeletal muscle interactome centered on proteins involved
            in muscular dystrophies: LGMD interactome
  JOURNAL   Skelet Muscle 3 (1), 3 (2013)
   PUBMED   23414517
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6308)
  AUTHORS   O'Seaghdha,C.M., Wu,H., Yang,Q., Kapur,K., Guessous,I., Zuber,A.M.,
            Kottgen,A., Stoudmann,C., Teumer,A., Kutalik,Z., Mangino,M.,
            Dehghan,A., Zhang,W., Eiriksdottir,G., Li,G., Tanaka,T., Portas,L.,
            Lopez,L.M., Hayward,C., Lohman,K., Matsuda,K., Padmanabhan,S.,
            Firsov,D., Sorice,R., Ulivi,S., Brockhaus,A.C., Kleber,M.E.,
            Mahajan,A., Ernst,F.D., Gudnason,V., Launer,L.J., Mace,A.,
            Boerwinckle,E., Arking,D.E., Tanikawa,C., Nakamura,Y., Brown,M.J.,
            Gaspoz,J.M., Theler,J.M., Siscovick,D.S., Psaty,B.M., Bergmann,S.,
            Vollenweider,P., Vitart,V., Wright,A.F., Zemunik,T., Boban,M.,
            Kolcic,I., Navarro,P., Brown,E.M., Estrada,K., Ding,J.,
            Harris,T.B., Bandinelli,S., Hernandez,D., Singleton,A.B.,
            Girotto,G., Ruggiero,D., d'Adamo,A.P., Robino,A., Meitinger,T.,
            Meisinger,C., Davies,G., Starr,J.M., Chambers,J.C., Boehm,B.O.,
            Winkelmann,B.R., Huang,J., Murgia,F., Wild,S.H., Campbell,H.,
            Morris,A.P., Franco,O.H., Hofman,A., Uitterlinden,A.G.,
            Rivadeneira,F., Volker,U., Hannemann,A., Biffar,R., Hoffmann,W.,
            Shin,S.Y., Lescuyer,P., Henry,H., Schurmann,C., Munroe,P.B.,
            Gasparini,P., Pirastu,N., Ciullo,M., Gieger,C., Marz,W., Lind,L.,
            Spector,T.D., Smith,A.V., Rudan,I., Wilson,J.F., Polasek,O.,
            Deary,I.J., Pirastu,M., Ferrucci,L., Liu,Y., Kestenbaum,B.,
            Kooner,J.S., Witteman,J.C., Nauck,M., Kao,W.H., Wallaschofski,H.,
            Bonny,O., Fox,C.S. and Bochud,M.
  CONSRTM   SUNLIGHT Consortium; GEFOS Consortium
  TITLE     Meta-analysis of genome-wide association studies identifies six new
            Loci for serum calcium concentrations
  JOURNAL   PLoS Genet 9 (9), e1003796 (2013)
   PUBMED   24068962
REFERENCE   5  (bases 1 to 6308)
  AUTHORS   Sato,M., Liu,K., Sasaki,S., Kunii,N., Sakai,H., Mizuno,H., Saga,H.
            and Sakane,F.
  TITLE     Evaluations of the selectivities of the diacylglycerol kinase
            inhibitors R59022 and R59949 among diacylglycerol kinase isozymes
            using a new non-radioactive assay method
  JOURNAL   Pharmacology 92 (1-2), 99-107 (2013)
   PUBMED   23949095
REFERENCE   6  (bases 1 to 6308)
  AUTHORS   Sakane,F., Imai,S., Yamada,K., Murakami,T., Tsushima,S. and
            Kanoh,H.
  TITLE     Alternative splicing of the human diacylglycerol kinase delta gene
            generates two isoforms differing in their expression patterns and
            in regulatory functions
  JOURNAL   J Biol Chem 277 (45), 43519-43526 (2002)
   PUBMED   12200442
  REMARK    GeneRIF: Alternative splicing of the gene generates two isoforms
            differing in their expression patterns and in regulatory functions
REFERENCE   7  (bases 1 to 6308)
  AUTHORS   Imai,S., Sakane,F. and Kanoh,H.
  TITLE     Phorbol ester-regulated oligomerization of diacylglycerol kinase
            delta linked to its phosphorylation and translocation
  JOURNAL   J Biol Chem 277 (38), 35323-35332 (2002)
   PUBMED   12084710
REFERENCE   8  (bases 1 to 6308)
  AUTHORS   Nagaya,H., Wada,I., Jia,Y.J. and Kanoh,H.
  TITLE     Diacylglycerol kinase delta suppresses ER-to-Golgi traffic via its
            SAM and PH domains
  JOURNAL   Mol Biol Cell 13 (1), 302-316 (2002)
   PUBMED   11809841
REFERENCE   9  (bases 1 to 6308)
  AUTHORS   Bregoli,L., Baldassare,J.J. and Raben,D.M.
  TITLE     Nuclear diacylglycerol kinase-theta is activated in response to
            alpha-thrombin
  JOURNAL   J Biol Chem 276 (26), 23288-23295 (2001)
   PUBMED   11309392
  REMARK    Erratum:[J Biol Chem 2002 Mar 29;277(13):11614-5]
REFERENCE   10 (bases 1 to 6308)
  AUTHORS   Sakane,F., Imai,S., Kai,M., Wada,I. and Kanoh,H.
  TITLE     Molecular cloning of a novel diacylglycerol kinase isozyme with a
            pleckstrin homology domain and a C-terminal tail similar to those
            of the EPH family of protein-tyrosine kinases
  JOURNAL   J Biol Chem 271 (14), 8394-8401 (1996)
   PUBMED   8626538
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC013726.7, D63479.3,
            BC006561.2 and AC019221.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_152879.2.
            
            Summary: This gene encodes a cytoplasmic enzyme that phosphorylates
            diacylglycerol to produce phosphatidic acid. Diacylglycerol and
            phosphatidic acid are two lipids that act as second messengers in
            signaling cascades. Their cellular concentrations are regulated by
            the encoded protein, and so it is thought to play an important role
            in cellular signal transduction. Alternative splicing results in
            two transcript variants encoding different isoforms. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.183865.1,
                                           SRR1803613.214186.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264057.7/ ENSP00000264057.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC013726.7         65856-65868         c
            14-73               AC013726.7         65796-65855         c
            74-4265             D63479.3           2-4193
            4266-6306           BC006561.2         1042-3082
            6307-6307           D63479.3           6238-6238
            6308-6308           AC019221.4         55675-55675         c
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..6308
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /note="diacylglycerol kinase delta"
                     /db_xref="GeneID:8527"
                     /db_xref="HGNC:HGNC:2851"
                     /db_xref="MIM:601826"
     exon            1..181
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..3670
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /EC_number="2.7.1.107"
                     /note="isoform 2 is encoded by transcript variant 2; DAG
                     kinase delta; diglyceride kinase delta; diacylglycerol
                     kinase, delta 130kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase delta isoform 2"
                     /protein_id="NP_690618.2"
                     /db_xref="CCDS:CCDS2504.1"
                     /db_xref="GeneID:8527"
                     /db_xref="HGNC:HGNC:2851"
                     /db_xref="MIM:601826"
                     /translation="MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVS
                     TSGQIRQKTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDAS
                     VAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHF
                     SGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTL
                     ASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHT
                     SCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQ
                     GVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDS
                     LNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMA
                     YEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLC
                     ETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNP
                     PPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQII
                     EHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSK
                     SPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISR
                     LLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNM
                     MWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGT
                     KEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPV
                     QVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEM
                     LSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTT
                     EGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLAD
                     TPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWG
                     TEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKE
                     LSRSAPAVEA"
     exon            182..292
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            293..373
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            374..478
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            479..611
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            612..718
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            719..844
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            845..947
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1110
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1219
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1359
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1444
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1539
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1639
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1913
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2063
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2192
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2289
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2400
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2497
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2498..2605
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2719
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2854
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..3006
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3007..3118
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3119..3211
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3331
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3332..3449
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3580
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3581..6308
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6280..6285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /note="hexamer: AATAAA"
     polyA_site      6308
                     /gene="DGKD"
                     /gene_synonym="DGK-delta; dgkd-2; DGKdelta"
                     /note="major polyA site"
ORIGIN      
        1 gcggtgcgcg cgctggcccg gcagcatggc ggcggcggcg ggcgcccctc cgccgggtcc
       61 cccgcaaccg cctccgccgc cgccgcccga ggagtcgtcc gacagcgagc ccgaggcgga
      121 gcccggctcc ccacagaagc tcatccgcaa ggtgtccacg tcgggtcaga tccgacagaa
      181 gaccatcatc aaagagggga tgctgaccaa acagaacaat tcattccagc gatcaaaaag
      241 gagatacttt aagcttcgag ggcgaacgct ttactatgcc aaaacggcaa agtcaatcat
      301 atttgatgag gtggatctga cagatgccag cgtagctgaa tccagtacca aaaacgtcaa
      361 caacagtttt acggtcataa ctccatgcag gaagctcatc ttgtgtgctg ataacagaaa
      421 agaaatggaa gattggattg cagcattaaa gactgtgcag aacagggagc actttgagcc
      481 cacccagtac agcatggacc acttctcagg gatgcacaat tggtacgcct gttcccacgc
      541 gaggccgacc tactgcaatg tgtgccgtga ggctctgtct ggggtcacgt cgcacgggct
      601 gtcctgcgag gtgtgcaaat ttaaggccca caagcgctgt gctgtgcgtg caaccaataa
      661 ctgcaagtgg accacactgg cctcgatcgg gaaggacatc attgaagatg cagatgggat
      721 tgcaatgccc caccagtggt tggaaggaaa cctacctgtg agcgccaagt gcactgtgtg
      781 cgacaagacc tgtggcagtg tgctgcgcct gcaggactgg cgctgcctct ggtgcaaggc
      841 catggttcac acatcgtgta aagaatcctt gctgaccaag tgcccacttg gcctgtgcaa
      901 agtgtcagtc atcccaccca cggctctcaa cagcatcgac tccgatgggt tctggaaggc
      961 cagctgtcct ccttcttgca caagcccact gttggtcttc gtcaattcaa aaagtgggga
     1021 caaccagggt gtgaagttcc tcagaagatt caaacagcta ctaaaccccg cccaggtctt
     1081 cgacctcatg aacggaggcc cacacctcgg cttacggtta ttccagaagt ttgacacatt
     1141 ccggattctg gtttgtggcg gggatggaag tgttggctgg gtcctctccg aaatcgacag
     1201 cctcaacctt cataaacagt gtcagctggg agtgctgccg ctcggcacag ggaacgactt
     1261 ggcccgagta ctgggctggg gctcagcctg cgatgacgac acccagctcc cccagatctt
     1321 ggagaagttg gagagagcca gcaccaagat gctggacagg tggagcgtca tggcatacga
     1381 ggccaagctc ccccggcagg cctcctcctc taccgtcacc gaagacttca gcgaggattc
     1441 cgaggtacag cagattctct tctatgaaga ctcggttgca gcccaccttt ctaaaatcct
     1501 cacctcggac cagcactcgg tggtcatctc ctcggccaaa gtgctctgtg agacggtgaa
     1561 ggacttcgtg gcacgggtgg ggaaggccta tgagaagacg accgagagct cggaggagtc
     1621 agaggtcatg gccaagaagt gctctgtcct gaaagagaag ctggattccc ttctcaagac
     1681 cttggacgat gagtcccagg cctcgtcctc tctgcccaac ccgcccccca ccattgccga
     1741 ggaggctgaa gatggagatg ggtcgggcag catctgcggt tccaccggag accgcttggt
     1801 ggcatcagct tgcccggccc ggccgcagat attccggcct cgagaacagc tcatgctgag
     1861 agccaacagc ctgaagaaag caattcgtca gatcatagaa cacacagaaa aagctgtcga
     1921 tgagcagaat gcccagaccc aggagcagga gggcttcgtc ctgggcctct ctgagtcaga
     1981 ggagaagatg gaccacagag tgtgcccacc actgtcccac agcgagagct tcggggtccc
     2041 caaggggagg agccagcgca aagtgtcgaa atctccgtgt gaaaagctga tcagcaaagg
     2101 gagtctgtcc ctaggcagtt ctgcttccct tccgccccag ccgggaagcc gggacggcct
     2161 gcctgcgctc aacaccaaga tcctgtaccc aaatgtccgg gctggaatgt ctggttcctt
     2221 acccggtggc tcagtcatca gtcgcctgtt aattaatgct gatcccttca actctgaacc
     2281 agaaacccta gagtattaca cggagaaatg tgtcatgaac aactattttg gcattggcct
     2341 ggatgcgaag atatccctgg actttaacaa caagcgcgat gagcacccag agaagtgcag
     2401 gagccgaacc aagaacatga tgtggtatgg agttcttgga accaaagagt tgctgcacag
     2461 aacctacaag aacctggagc aaaaggtctt gctggagtgt gacgggcgac ccatcccact
     2521 ccccagtctt cagggaattg ctgtccttaa cattcccagc tatgccggag gaaccaactt
     2581 ctgggggggt accaaggaag atgatacttt cgcagctcca tcattcgatg acaagattct
     2641 ggaggtggtc gccgtgttcg gcagcatgca gatggccgtc tctcgagtca tcaggctaca
     2701 gcatcatcgg atcgcccagt gtcgcacggt gaagatctcc atccttgggg atgagggcgt
     2761 gcctgtgcag gtggacggag aggcctgggt ccagccgcca gggtacattc ggattgtcca
     2821 caagaaccgg gcacagacac tgaccagaga cagggcattt gagagcaccc tgaagtcctg
     2881 ggaagacaag cagaagtgcg agctgccccg ccctccatcc tgttccctgc acccggagat
     2941 gctgtccgag gaggaggcca cccagatgga ccagtttggg caggcagcag gggtcctcat
     3001 tcacagtatc cgagaaatag ctcagtctca ccgggacatg gagcaggaac tggcccacgc
     3061 cgtcaatgcc agctccaagt ccatggaccg tgtgtatggc aagcccagaa ccacagaggg
     3121 gctcaactgc agcttcgtcc tggaaatggt gaataacttc agagctctgc gcagtgagac
     3181 ggagctgctg ctgtctggga agatggccct gcagctggat ccgcctcaga aggagcagct
     3241 ggggagtgct cttgccgaga tggaccgaca gctcaggagg ctggcagaca ccccgtggct
     3301 ctgccagtcc gcagagcccg gcgacgaaga gagtgtgatg ctggatcttg ccaagcgcag
     3361 tcgcagtggt aaattccgcc tcgtgaccaa gtttaaaaag gagaaaaaca acaagaacaa
     3421 agaagctcac agtagcctgg gagccccggt tcacctctgg gggacagagg aggttgctgc
     3481 ctggctggag cacctcagtc tctgtgagta taaggacatc ttcacacggc acgacatccg
     3541 gggctctgag ctcctgcacc tggagcggag ggacctcaag gacctgggcg tgaccaaggt
     3601 gggccacatg aagaggatcc tgtgtggcat caaggagctg agccgcagcg cccccgccgt
     3661 cgaggcctag cctctgtcct ctcagcctgt ggcctccaca tccccgccgc cgaggcctag
     3721 cctccgccct ctcagcctgt ggcctctgcg cctcctgcca ctgaggccct gggcagatgc
     3781 tgcagcccgc ccccttctca tggtgctact tcctctgtca gctacagaaa gcctccgtga
     3841 caccgtccac cagagctctg gggtctcgaa cataacaaca cagctacctt tgaaacaaca
     3901 ctttctccag ctcagagtca cctggggcac atgtgtcacg gccactcagc tctcgcccgc
     3961 ctgtgctgtg ggccagggaa tccagcggcg tctggcctcc tgggcactgc ttgcctggcc
     4021 tcgtgcttgg attgtcccgg gggctcctct ccgtgtgtcc ttctgtggcc gcaccgtgtg
     4081 gctccgcctc ctggccccca gccagttctc agaaacgtgg ctggggccca gcacagcagc
     4141 ctgcaagggc ccctgtttgt tgatgcagct tttgttgaac aaaaatcgtg ctctttcctg
     4201 gtttgaaagt agcatggatg tttccagtct tgttgattgt aatttgacgt gaagagaaaa
     4261 aaaaattcct cctgcgtgag ccaaggcagc gggtgctgtt tcccaggcgg ggagcccctc
     4321 cctgggtgtc acagggcctg tgctcctccc tcctccatcc tctctcctcc cgctcctccc
     4381 tccccccact gtgggctggg gacgcctgcc cttctgtctc cggacgctct aggcgagttc
     4441 agcttggggt gtgagtgaga cagcttgcca gctgcatccc tgcagacaga ggatgtgtgt
     4501 ccacatgagt gtttctgtgt gggaaatgct tcctggctct gggaaacttt ttctgcccat
     4561 tctgtggttc ccagggagcg tggccctggt gggccagggg tggtttgacc tcttcagccc
     4621 gtccggtggc ctggaggccg gaggctctcc tgagtgtctg cccctgcagt ggcttcttgt
     4681 cgcctgctgc tgggcgtgat gtcgctggag gtgctggcag ggactctgat ttggtggtcc
     4741 gcgctgcccc tgccctgcct ctgtcctggc tctgaactag tagatgatgg tgccagaggg
     4801 cagggagctc gcctggggag agggctgtgc cccgtaggga cagtgcccag gtgaaggatg
     4861 cccctggtcc tccagggcac tgactttgcc cttttttccc gttgatagtc atggctcaga
     4921 ggtgcttgta aatgtcttgg gaagaggttt ctgtaacccc tgccctggtg tgaggaggaa
     4981 atggctctgg cctggctgcc tggccgtggc ttctctttgg ctcccaaaga gaaggacagt
     5041 gttgggagta tctgccgtgg cttctctttg gctcccaaag agaaggacag tgttgggagt
     5101 atctgccggc gctgtccagg tcctttagtc agcgtcactc catctgatgt gcagaagctg
     5161 ggctgcacct gcgggggtgg gcatagaccg ggctgggtct gcagcagccc ctggtcctga
     5221 gcaggcggca gtgaacagca ctggcccacc tcccactcac agcccctctg tcccctctgc
     5281 agtgcaccca ggtgggcccc tctgcgtgcc tttgggtgct cccctctcgt ggtcgttctg
     5341 gcccgaggcc cttagagtat ggaggctgag ccaggccttg ggtttcccca gcacagcctc
     5401 ctgtcgctgc atgcgacgtg ttgggatttt tggatgaaag actctcccac gctctgttgg
     5461 tggacttagc tgcctcactg gaagtgatgt gggtggaagg tggttgtatg ttaccttttc
     5521 cacctctcat tgttttcccc agaacattgt agatgggggt tggcagaggg agaaataagc
     5581 cagccacggc agtcgcttgg tttcccaggt ggaatgggct aacacaggag atgatgggaa
     5641 cctgtcccgc agtccctgca tgaccattgg ccctgctggc ctggcgatgt gggcatcctg
     5701 gggttcttag ggtcccagaa caagccccag gcaagctgga acttgggtgg ggaggggaca
     5761 tgaggaggat aaacagctga ctgtggcttc aaggacatca gggccacccc aagtcctcag
     5821 tgtcctactc ctggcaagga gttgggtttg gatcaaaagt gtttaaaatt aatatgttgt
     5881 cagtgattag aacaacactg tttacataaa aaccattttt ctaattctaa caagttagaa
     5941 tgtgaggaag gaatgaacat gagtgtttag gaacctgccc tttggtgctg ggctggcgtc
     6001 ccgcactggg gtgtcctcgc tgtctggggg ctgctctgct gccccggccc aggtcccctt
     6061 gtggtgttgc cagacgggcc tcatggtctg ctgtgcagag agaggcagga aggatccctg
     6121 aagagtcttg gagaaaaggt tctgtgccct caggtggggc ttaccccctc gtatttataa
     6181 tcttaattta tatagtgacc accgtggaaa caaacgcctc ttgtattgtc atgtacatag
     6241 tccatacctg agtgctgtac ataagttgtt ctgtgtataa ataaaacaag cctgtttttg
     6301 atcttcca
//
