LOCUS       NM_001350709            6841 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens diacylglycerol kinase beta (DGKB), transcript variant
            6, mRNA.
ACCESSION   NM_001350709 XM_017011783
VERSION     NM_001350709.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Kang,H., Lee,H., Kim,K., Shin,E., Kim,B., Kang,J., Kim,B.,
            Lee,J.S., Lee,J.M., Youn,H. and Youn,B.
  TITLE     DGKB mediates radioresistance by regulating DGAT1-dependent
            lipotoxicity in glioblastoma
  JOURNAL   Cell Rep Med 4 (1), 100880 (2023)
   PUBMED   36603576
  REMARK    GeneRIF: DGKB mediates radioresistance by regulating
            DGAT1-dependent lipotoxicity in glioblastoma.
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Kano,T., Tsumagari,R., Nakashima,A., Kikkawa,U., Ueda,S.,
            Yamanoue,M., Takei,N. and Shirai,Y.
  TITLE     RalA, PLD and mTORC1 Are Required for Kinase-Independent Pathways
            in DGKbeta-Induced Neurite Outgrowth
  JOURNAL   Biomolecules 11 (12), 1814 (2021)
   PUBMED   34944458
  REMARK    GeneRIF: RalA, PLD and mTORC1 Are Required for Kinase-Independent
            Pathways in DGKbeta-Induced Neurite Outgrowth.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6841)
  AUTHORS   Peiris,H., Park,S., Louis,S., Gu,X., Lam,J.Y., Asplund,O.,
            Ippolito,G.C., Bottino,R., Groop,L., Tucker,H. and Kim,S.K.
  TITLE     Discovering human diabetes-risk gene function with genetics and
            physiological assays
  JOURNAL   Nat Commun 9 (1), 3855 (2018)
   PUBMED   30242153
  REMARK    GeneRIF: RNA interference-mediated knockdown of the Drosophila
            ortholog of DGKB in insulin-producing cells, a model for mammalian
            beta cells, conferred increased insulin output.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6841)
  AUTHORS   Tarnowski,M., Malinowski,D., Pawlak,K., Dziedziejko,V., Safranow,K.
            and Pawlik,A.
  TITLE     GCK, GCKR, FADS1, DGKB/TMEM195 and CDKAL1 Gene Polymorphisms in
            Women with Gestational Diabetes
  JOURNAL   Can J Diabetes 41 (4), 372-379 (2017)
   PUBMED   28359772
REFERENCE   5  (bases 1 to 6841)
  AUTHORS   Mishima,S., Matsuda,C., Ishihara,T., Nagase,M., Taketani,T. and
            Nagai,A.
  TITLE     Single nucleotide polymorphisms of the DGKB and VCAM1 genes are
            associated with granulocyte colony stimulating factor-mediated
            peripheral blood stem cell mobilization
  JOURNAL   Transfus Apher Sci 56 (2), 154-159 (2017)
   PUBMED   27839966
  REMARK    GeneRIF: Association with the efficiency of hematopoietic stem
            cells (HSC) mobilization to PB were found in the SNPs of the DGKB
            gene involved in cell transport and SDF-1-induced migration ability
            and of the VCAM1 gene which is essential for HSC homing, suggesting
            that SNPs involved in cell migration ability might be partly
            involved in HSC mobilization to peripheral blood.
REFERENCE   6  (bases 1 to 6841)
  AUTHORS   Dupuis,J., Langenberg,C., Prokopenko,I., Saxena,R., Soranzo,N.,
            Jackson,A.U., Wheeler,E., Glazer,N.L., Bouatia-Naji,N., Gloyn,A.L.,
            Lindgren,C.M., Magi,R., Morris,A.P., Randall,J., Johnson,T.,
            Elliott,P., Rybin,D., Thorleifsson,G., Steinthorsdottir,V.,
            Henneman,P., Grallert,H., Dehghan,A., Hottenga,J.J., Franklin,C.S.,
            Navarro,P., Song,K., Goel,A., Perry,J.R., Egan,J.M., Lajunen,T.,
            Grarup,N., Sparso,T., Doney,A., Voight,B.F., Stringham,H.M., Li,M.,
            Kanoni,S., Shrader,P., Cavalcanti-Proenca,C., Kumari,M., Qi,L.,
            Timpson,N.J., Gieger,C., Zabena,C., Rocheleau,G., Ingelsson,E.,
            An,P., O'Connell,J., Luan,J., Elliott,A., McCarroll,S.A., Payne,F.,
            Roccasecca,R.M., Pattou,F., Sethupathy,P., Ardlie,K., Ariyurek,Y.,
            Balkau,B., Barter,P., Beilby,J.P., Ben-Shlomo,Y., Benediktsson,R.,
            Bennett,A.J., Bergmann,S., Bochud,M., Boerwinkle,E., Bonnefond,A.,
            Bonnycastle,L.L., Borch-Johnsen,K., Bottcher,Y., Brunner,E.,
            Bumpstead,S.J., Charpentier,G., Chen,Y.D., Chines,P., Clarke,R.,
            Coin,L.J., Cooper,M.N., Cornelis,M., Crawford,G., Crisponi,L.,
            Day,I.N., de Geus,E.J., Delplanque,J., Dina,C., Erdos,M.R.,
            Fedson,A.C., Fischer-Rosinsky,A., Forouhi,N.G., Fox,C.S.,
            Frants,R., Franzosi,M.G., Galan,P., Goodarzi,M.O., Graessler,J.,
            Groves,C.J., Grundy,S., Gwilliam,R., Gyllensten,U., Hadjadj,S.,
            Hallmans,G., Hammond,N., Han,X., Hartikainen,A.L., Hassanali,N.,
            Hayward,C., Heath,S.C., Hercberg,S., Herder,C., Hicks,A.A.,
            Hillman,D.R., Hingorani,A.D., Hofman,A., Hui,J., Hung,J.,
            Isomaa,B., Johnson,P.R., Jorgensen,T., Jula,A., Kaakinen,M.,
            Kaprio,J., Kesaniemi,Y.A., Kivimaki,M., Knight,B., Koskinen,S.,
            Kovacs,P., Kyvik,K.O., Lathrop,G.M., Lawlor,D.A., Le Bacquer,O.,
            Lecoeur,C., Li,Y., Lyssenko,V., Mahley,R., Mangino,M.,
            Manning,A.K., Martinez-Larrad,M.T., McAteer,J.B., McCulloch,L.J.,
            McPherson,R., Meisinger,C., Melzer,D., Meyre,D., Mitchell,B.D.,
            Morken,M.A., Mukherjee,S., Naitza,S., Narisu,N., Neville,M.J.,
            Oostra,B.A., Orru,M., Pakyz,R., Palmer,C.N., Paolisso,G.,
            Pattaro,C., Pearson,D., Peden,J.F., Pedersen,N.L., Perola,M.,
            Pfeiffer,A.F., Pichler,I., Polasek,O., Posthuma,D., Potter,S.C.,
            Pouta,A., Province,M.A., Psaty,B.M., Rathmann,W., Rayner,N.W.,
            Rice,K., Ripatti,S., Rivadeneira,F., Roden,M., Rolandsson,O.,
            Sandbaek,A., Sandhu,M., Sanna,S., Sayer,A.A., Scheet,P.,
            Scott,L.J., Seedorf,U., Sharp,S.J., Shields,B., Sigurethsson,G.,
            Sijbrands,E.J., Silveira,A., Simpson,L., Singleton,A., Smith,N.L.,
            Sovio,U., Swift,A., Syddall,H., Syvanen,A.C., Tanaka,T.,
            Thorand,B., Tichet,J., Tonjes,A., Tuomi,T., Uitterlinden,A.G., van
            Dijk,K.W., van Hoek,M., Varma,D., Visvikis-Siest,S., Vitart,V.,
            Vogelzangs,N., Waeber,G., Wagner,P.J., Walley,A., Walters,G.B.,
            Ward,K.L., Watkins,H., Weedon,M.N., Wild,S.H., Willemsen,G.,
            Witteman,J.C., Yarnell,J.W., Zeggini,E., Zelenika,D., Zethelius,B.,
            Zhai,G., Zhao,J.H., Zillikens,M.C., Borecki,I.B., Loos,R.J.,
            Meneton,P., Magnusson,P.K., Nathan,D.M., Williams,G.H.,
            Hattersley,A.T., Silander,K., Salomaa,V., Smith,G.D.,
            Bornstein,S.R., Schwarz,P., Spranger,J., Karpe,F., Shuldiner,A.R.,
            Cooper,C., Dedoussis,G.V., Serrano-Rios,M., Morris,A.D., Lind,L.,
            Palmer,L.J., Hu,F.B., Franks,P.W., Ebrahim,S., Marmot,M., Kao,W.H.,
            Pankow,J.S., Sampson,M.J., Kuusisto,J., Laakso,M., Hansen,T.,
            Pedersen,O., Pramstaller,P.P., Wichmann,H.E., Illig,T., Rudan,I.,
            Wright,A.F., Stumvoll,M., Campbell,H., Wilson,J.F., Bergman,R.N.,
            Buchanan,T.A., Collins,F.S., Mohlke,K.L., Tuomilehto,J.,
            Valle,T.T., Altshuler,D., Rotter,J.I., Siscovick,D.S.,
            Penninx,B.W., Boomsma,D.I., Deloukas,P., Spector,T.D.,
            Frayling,T.M., Ferrucci,L., Kong,A., Thorsteinsdottir,U.,
            Stefansson,K., van Duijn,C.M., Aulchenko,Y.S., Cao,A., Scuteri,A.,
            Schlessinger,D., Uda,M., Ruokonen,A., Jarvelin,M.R.,
            Waterworth,D.M., Vollenweider,P., Peltonen,L., Mooser,V.,
            Abecasis,G.R., Wareham,N.J., Sladek,R., Froguel,P., Watanabe,R.M.,
            Meigs,J.B., Groop,L., Boehnke,M., McCarthy,M.I., Florez,J.C. and
            Barroso,I.
  CONSRTM   DIAGRAM Consortium; GIANT Consortium; Global BPgen Consortium;
            Anders Hamsten on behalf of Procardis Consortium; MAGIC
            investigators
  TITLE     New genetic loci implicated in fasting glucose homeostasis and
            their impact on type 2 diabetes risk
  JOURNAL   Nat Genet 42 (2), 105-116 (2010)
   PUBMED   20081858
  REMARK    GeneRIF: Meta-analysis and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Erratum:[Nat Genet.2010 May;42(5):464]
REFERENCE   7  (bases 1 to 6841)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 6841)
  AUTHORS   Yang,J.J., Cheng,C., Yang,W., Pei,D., Cao,X., Fan,Y., Pounds,S.B.,
            Neale,G., Trevino,L.R., French,D., Campana,D., Downing,J.R.,
            Evans,W.E., Pui,C.H., Devidas,M., Bowman,W.P., Camitta,B.M.,
            Willman,C.L., Davies,S.M., Borowitz,M.J., Carroll,W.L., Hunger,S.P.
            and Relling,M.V.
  TITLE     Genome-wide interrogation of germline genetic variation associated
            with treatment response in childhood acute lymphoblastic leukemia
  JOURNAL   JAMA 301 (4), 393-403 (2009)
   PUBMED   19176441
REFERENCE   9  (bases 1 to 6841)
  AUTHORS   Merida,I., Avila-Flores,A. and Merino,E.
  TITLE     Diacylglycerol kinases: at the hub of cell signalling
  JOURNAL   Biochem J 409 (1), 1-18 (2008)
   PUBMED   18062770
  REMARK    Review article
REFERENCE   10 (bases 1 to 6841)
  AUTHORS   Caricasole,A., Bettini,E., Sala,C., Roncarati,R., Kobayashi,N.,
            Caldara,F., Goto,K. and Terstappen,G.C.
  TITLE     Molecular cloning and characterization of the human diacylglycerol
            kinase beta (DGKbeta) gene: alternative splicing generates DGKbeta
            isotypes with different properties
  JOURNAL   J Biol Chem 277 (7), 4790-4796 (2002)
   PUBMED   11719522
  REMARK    GeneRIF: human diacylglycerol kinase beta gene can generate several
            enzyme isoforms, which can display different expression levels and
            subcellular localization but similar enzymatic activities in vitro.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006045.2, AC006150.2,
            AC073258.9, AC105459.1, AC011229.2, AC005248.1 and AC005039.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001350709.1.
            
            Summary: Diacylglycerol kinases (DGKs) are regulators of the
            intracellular concentration of the second messenger diacylglycerol
            (DAG) and thus play a key role in cellular processes. Nine
            mammalian isotypes have been identified, which are encoded by
            separate genes. Mammalian DGK isozymes contain a conserved
            catalytic (kinase) domain and a cysteine-rich domain (CRD). The
            protein encoded by this gene is a diacylglycerol kinase, beta
            isotype. Several alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Apr 2017].
            
            Transcript Variant: This variant (6), as well as variants 4 and 5,
            encodes isoform 3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.26531.1, SRR1660805.45.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402815.6/ ENSP00000384909.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AC006045.2         45341-45500         c
            161-417             AC006150.2         96828-97084         c
            418-494             AC006150.2         13289-13365         c
            495-515             AC006150.2         9562-9582           c
            516-669             AC073258.9         147602-147755       c
            670-813             AC073258.9         130103-130246       c
            814-863             AC073258.9         113242-113291       c
            864-938             AC073258.9         109656-109730       c
            939-1058            AC073258.9         105636-105755       c
            1059-1176           AC073258.9         96806-96923         c
            1177-1265           AC073258.9         94314-94402         c
            1266-1382           AC073258.9         94114-94230         c
            1383-1481           AC073258.9         84490-84588         c
            1482-1514           AC073258.9         41797-41829         c
            1515-1631           AC073258.9         32939-33055         c
            1632-1705           AC073258.9         24901-24974         c
            1706-1780           AC073258.9         18995-19069         c
            1781-1866           AC105459.1         138711-138796       c
            1867-1956           AC105459.1         136521-136610       c
            1957-2117           AC105459.1         129871-130031       c
            2118-2182           AC105459.1         33820-33884         c
            2183-2273           AC011229.2         100951-101041
            2274-2469           AC005248.1         2792-2987
            2470-2590           AC005039.1         95087-95207
            2591-2651           AC005039.1         96339-96399
            2652-6841           AC005039.1         124000-128189
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p21.2"
     gene            1..6841
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /note="diacylglycerol kinase beta"
                     /db_xref="GeneID:1607"
                     /db_xref="HGNC:HGNC:2850"
                     /db_xref="MIM:604070"
     exon            1..160
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            161..417
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     CDS             348..2759
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /EC_number="2.7.1.107"
                     /note="isoform 3 is encoded by transcript variant 6; DAG
                     kinase beta; diglyceride kinase beta; 90 kDa
                     diacylglycerol kinase; diacylglycerol kinase, beta 90kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase beta isoform 3"
                     /protein_id="NP_001337638.1"
                     /db_xref="CCDS:CCDS87481.1"
                     /db_xref="GeneID:1607"
                     /db_xref="HGNC:HGNC:2850"
                     /db_xref="MIM:604070"
                     /translation="MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAK
                     YNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPMVKSKP
                     ALLSGGLRMNKGAITPPRTTSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRL
                     YDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEE
                     WIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCS
                     FCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGL
                     TGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTLPTSGVSVPEERQ
                     STVKKEKSGSQQPNKVIDKNKMQRANSVTVDGQGLQVTPVPGTHPLLVFVNPKSGGKQ
                     GERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEK
                     ANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKFEVI
                     PNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGT
                     SETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSH
                     RRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLA
                     QCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLVKRTR
                     NRSKE"
     exon            418..494
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            495..515
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            516..669
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            670..813
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            814..863
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            864..938
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1058
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1176
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1265
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1382
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1481
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1514
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1631
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1705
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1780
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1866
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..1956
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2117
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2182
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2273
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2469
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2590
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2651
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..6841
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4252..4257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /note="hexamer: ATTAAA"
     polyA_site      4277
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /note="major polyA site"
     regulatory      6821..6826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /note="hexamer: AATAAA"
     polyA_site      6841
                     /gene="DGKB"
                     /gene_synonym="DAGK2; DGK; DGK-BETA"
                     /note="major polyA site"
ORIGIN      
        1 actcctctgg ggaagccgag ctgtgtggga gccttcttac tgtgccggga gcgtgtgaat
       61 tggaaaggat cctgagaact ggctagtccc agttcctctc cggaaaagca gtggctctcg
      121 cttcagagat gcgctcagct ttcgcctgca tcacactgca ttctttaaca cattattgaa
      181 attacaagca tccaaagcag tttcatgtgg acagattgca tattttgaaa gcctgaggta
      241 ttttatcatg aaacatgcca tgtggaatct ttgaagcata gacctctgcg caacacctga
      301 ataaagaatc ttttacctgg tatgtgacag agcttctcac caccaccatg acaaaccagg
      361 aaaaatgggc ccacctcagc ccttcggaat tttcccaact tcagaaatat gctgagtatt
      421 ctacaaagaa attaaaggat gttcttgaag aattccatgg taatggtgtg cttgcaaagt
      481 ataatcctga agggaaacaa gacattctta accaaacaat agattttgaa ggtttcaaac
      541 tattcatgaa gacattcctg gaagccgagc ttcctgatga tttcactgca caccttttca
      601 tgtcatttag caacaagttt cctcattcta gtccaatggt aaaaagtaag cctgctctcc
      661 tatcaggcgg tctgagaatg aataaaggtg ccatcacccc tccccggact acttctcctg
      721 caaatacgtg ttccccagaa gtaatccatc tgaaggacat tgtctgttac ctgtctctgc
      781 ttgaaagagg aagacctgag gataagcttg agtttatgtt tcgcctttat gacacggatg
      841 ggaatggctt cctggacagc tcggagctag aaaatatcat cagtcagatg atgcatgttg
      901 cagaatacct tgagtgggat gtcactgaac ttaatccaat cctccatgaa atgatggaag
      961 aaattgacta tgatcatgat ggaaccgtgt ctctggagga atggattcaa ggaggaatga
     1021 caacgattcc acttcttgtg ctcctgggct tagaaaataa cgtgaaggat gatggacagc
     1081 acgtgtggcg actgaagcac tttaacaaac ctgcctattg caacctttgc ctgaacatgc
     1141 tgattggcgt ggggaagcag ggcctctgct gttccttctg caagtacaca gtccatgagc
     1201 gctgtgtggc tcgagcacct ccctcttgca tcaagaccta tgtgaagtcc aaaaggaaca
     1261 ctgatgtcat gcaccattac tgggttgaag gtaactgccc aaccaagtgt gataagtgcc
     1321 acaaaactgt taaatgttac cagggcctga caggactgca ttgtgtttgg tgtcagatca
     1381 cactgcataa taaatgtgct tctcatctaa aacctgaatg tgactgtgga cctttgaagg
     1441 accatatttt accacccaca acaatctgtc cagtggtact gactctgccc acttcaggag
     1501 tttcagttcc tgaggaaaga caatcaacag tgaaaaagga aaagagtggt tcccagcagc
     1561 caaacaaagt gattgacaag aataaaatgc aaagagccaa ctctgttact gtagatggac
     1621 aaggcctgca ggtcactcct gtgcctggta ctcacccact tttagttttt gtgaacccca
     1681 aaagtggtgg aaaacaagga gaacgaattt acagaaaatt ccagtatcta ttaaatcctc
     1741 gtcaggttta cagtctttct ggaaatggac caatgccagg gttaaacttt ttccgtgatg
     1801 ttcctgactt cagagtgtta gcctgtggtg gagatggaac cgtgggctgg gttttggatt
     1861 gcatagaaaa ggccaatgta ggcaagcatc ctccagttgc gattctgcct cttgggactg
     1921 gcaatgatct agcaagatgc ctgcgatggg gaggaggtta cgaaggtgag aatctgatga
     1981 aaattctaaa agacattgaa aacagcacag aaatcatgtt ggacaggtgg aagtttgaag
     2041 tcatacctaa tgacaaagat gagaaaggag acccagtgcc ttacagtatc atcaataatt
     2101 acttttccat tggcgtggat gcctccattg cacacagatt ccacatcatg agagaaaaac
     2161 acccagagaa attcaacagt agaatgaaga acaaattttg gtattttgag tttggcacat
     2221 ctgaaacttt ctcagccacc tgcaagaagc tacatgaatc tgtagaaata gaatgtgatg
     2281 gagtacagat agatttaata aacatctctc tggaaggaat tgctattttg aatataccaa
     2341 gcatgcatgg aggatccaat ctttggggag agtctaagaa aagacgaagc catcgacgaa
     2401 tagagaaaaa agggtctgac aaaaggacca ccgtcacaga tgccaaagag ttgaagtttg
     2461 caagtcaaga tctcagtgac cagctgctgg aggtggtcgg cttggaagga gccatggaga
     2521 tggggcaaat atacacaggc ctgaaaagtg ctggccggcg gctggctcag tgctcctgcg
     2581 tggtcatcag gacgagcaag tctctgccaa tgcaaattga tggggagcca tggatgcaga
     2641 ccccatgcac aataaaaatt acacacaaga accaagcccc aatgctgatg ggcccgcctc
     2701 caaaaaccgg tttattctgc tccctcgtca aaaggacaag aaaccgaagc aaggaataat
     2761 cctgtgttgt ttcactctta gaaattgaat tagcataatt gggccatgga acacatatgc
     2821 tggaaatctt tgaaccattt caagtctcct gctcatgcaa aatcatggaa gtggtttaac
     2881 agtttttgtt actaagctaa tgtaaaattc agctattaga aaatttattg tctcagtttt
     2941 tataggcatc tttgcatgaa gaaagcagaa gtttacctga agtgatactg catatttttg
     3001 gtgcatgcat tcccatagat ttttacatct cccacccaac tcttccccaa tttcctttta
     3061 ctaacctgtg agaaaaaccc gtgaaacatg aaaaaggaaa taccatggga aacgtgattc
     3121 tcagtgtgat tccaattatt acgaagcact aatcagtaac gctacaatga tcataattgc
     3181 agattgctat acgtttccct tttagaatca gtgtatcagt gacctatgac ttgaggagaa
     3241 acttttaatt cgaagatttt attaaatagt tgactacaat accttgctat atatacatag
     3301 tttttcttca acatcttaac tcttctgagt ggaaataaaa atatcaggca taaggttttc
     3361 tcatgctgaa aaatagaacg cggtttttat tttgcttagt tttcttttta attccagaaa
     3421 taagtgaaaa catgttactt gacagtcaag tgtggtaata tggcaagcct tgttcctttc
     3481 tgcatgagaa tctaggagag aattcataac cacaccaata acgaaataga agttttaaac
     3541 tatgtgccta atcaatgtgt ttcccaccaa agattcagaa aacaatgctt gagagaaatg
     3601 ggttaatgca taattaatta agcattgtgg agcaaattta gggttcctgt gattaatttt
     3661 gtgatgacta aaatgctgga aagcgagtga gttgcccatt aattatgatt aaaattctca
     3721 cctttcacag acagacaata agccagacaa cacaatcaaa gctcaataga tgatttcttg
     3781 cttttttcag tcatttataa atataggtgt aatttttcat ggatcagtta agtacacttg
     3841 aaggaagtaa atgattgtat cagtttattt ctagtataaa tgggtacctg taataatact
     3901 gagctcttgg aagcgaatca tgcatgcaat tagctccctc ctcctcacct actccactcc
     3961 catctttatg acatttcaaa tgtttatttg gaaacaacag cctagatcac tgttgaaggt
     4021 gttcatggca tagttggagt ctctgactgt ttaaagaaat cacagaacag tacttttctt
     4081 ttagtgtttc attaagccta tgatgtaaaa tgaaatgctt ctgagcagtc ttgtaatatt
     4141 gttcattcat attgacctgc atctcatcat tgcatgtttt atgttttcaa acatgccata
     4201 aggaaaacga gtgcctgaac tgcatgattt attagtttct ctccactctg cattaaagtg
     4261 ctaatgattt atcggttcta ttttgttatt gattcattca tctttctttg aataacaaat
     4321 agcatttagt gattctgttg aacacaatcc tgtgcatgga tttatggatt tcaagtgaaa
     4381 ttgctataat tttagttctt tggtttgaaa actcaactga atgctgttat atcaaatagc
     4441 tgctctcaag caatgtgcta taatggaaga ttacgtacaa agtgtattct tttagtgttc
     4501 ctggaacatt ggacggtaaa tatcaatcaa caataagctt aattacttta aaaataaaag
     4561 cttttgaata aaattaagac ataatttagc attcccagga aaattatgat tacaattgca
     4621 aatttagtac agcaatcact tgcgctggta taaaataaaa aagttgtttg gcagaagagt
     4681 aatccagacc aaaagaaaga aagaaatctc aaaacagtaa caagtacatt actgacataa
     4741 tgttacgtaa cacacacatt gattttttgt atctatggtt gcaggtaata aatattttca
     4801 catgaattga caaaattttc tacaatgggc aggcaggctt tgtgtcaaaa acatattttg
     4861 aagccacaaa ttattttatg ttcgctacaa catacaatta tgtcttggct accttttata
     4921 gtatatagtt tgaaggcagc aacagtttgt gaatcattct taacaatctg tacagtagaa
     4981 agctgttgga tagacagtgg gatgagacaa attaaacaac ctgcttataa gatttcaatg
     5041 attgaaataa tgggatgcag cttcacttca gtgtagctgt ataactacaa ttagtgctaa
     5101 tagtggttac attttcagtc agatggattc tttggtaccc ataataaatg ttcaaatagg
     5161 aatccaattt gacttttctc aactcatttt ctaaatacac tgatgtactt gccccttcaa
     5221 gtatactgcc cctggggttt ggaaagtagt ttttaaaaac gtaacttgat gggtttgaaa
     5281 tcagagaaga tattctcatc agttttatga cagcttcata tgaaaaatat atagtttaag
     5341 gaaaattcta tagtatatta aaatattgaa tgatgaatgt tgttagcttt acaagatgga
     5401 aacttctctt ctatgtgtgc agataaattt tcagctattg agtcgccctc ttttaatata
     5461 ggagacataa agttttaagt ggctgaaact cttaaatgta aattaatgct ggacttaaca
     5521 gcagtcagaa acaaagaggg aaaagagaca tgcactctgc taaacattgc taggcactga
     5581 gttgatgaga ttccagagag acatttcaga gtaaaccttg atatccacgc ttctaagaga
     5641 taggaaggga ggtatctaca tagaaccaga aagtttaaaa gacgtaaatt tattttttcc
     5701 acttccttgt gtacaccatg tatttcatgt gaaaatgctg atcaatttat tgtggtggaa
     5761 ggagatatct tgcaggaagt ttaaaaatta acattttaaa acagttttta ttttatttaa
     5821 aacaggttca tgtgatcata gccagaataa aacaaaaggc tcagatgttt agcctagtct
     5881 actgcagaca gaaatttaaa tccagtgagg gctaatgaat cacatacgtg tttccacatt
     5941 ttattttgtt ttcgtttttt aggcatatgg gaagtagttg caatttttca gggaagaata
     6001 tgttttgaat ctcacactgt attctccttg aatctcacac tgtattcttg aatgagacaa
     6061 gattttgaga tcattctttt cctttttgtg tcaacgttag tttgttgttc attggttata
     6121 aaaaggaaaa tgtggccagg cgcaatggct tacgcctgta atcccagcac tttgggagac
     6181 agaggcaggc agatcacaag gtcaggagtt caagaccagc ctggccaata tggtgaaacc
     6241 tcgtctctac taaaaataca aaaattagcc aggcatggtg gcacatgcct gtaatcccag
     6301 ctactcggga ggctgaggca gaagaattgc ttgaactcgg gaggctgcgg ttgcagtgag
     6361 ccaagatcac accactgcac tccagcctgg gtgacagagc cagactctgt ctcaaaaaaa
     6421 aaaaggaaaa tgtacttttc atggcacgta taacccccaa aggattattc aatactgaac
     6481 aattaaataa taattgaata atattgaatc atgttctccg ttatttttca aataactgaa
     6541 taatatctct attaaaagat taaacccttg taacatatat taaagctgta aactttaaga
     6601 aaataaataa ataaaaccct gtaaaagagc ttttattttg tgagaggcag aaatcaccac
     6661 ttaaaactcc caattaaatt ctgctatatt taaataaagt ttgagtaaac tcacatttga
     6721 gagattctgt ataaccttat tgtgtttaaa aacatggaac ttaattatag taccttaatt
     6781 attttctcag aaatgatata tacaatatcc tgttttttga aataaaaacg ttaaccactc
     6841 a
//
