LOCUS       NM_001413603            2652 bp    mRNA    linear   PRI 14-FEB-2025
DEFINITION  Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant
            22, mRNA.
ACCESSION   NM_001413603
VERSION     NM_001413603.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Shichi,S., Sugiyama,K., Asahi,Y., Shirakawa,C., Nakamoto,H.,
            Kimura,S., Wakizaka,K., Aiyama,T., Nagatsu,A., Orimo,T.,
            Kakisaka,T. and Taketomi,A.
  TITLE     Diacylglycerol kinase alpha is a proliferation marker of
            intrahepatic cholangiocarcinoma associated with the prognosis
  JOURNAL   Cancer Med 13 (9), e7238 (2024)
   PUBMED   38716625
  REMARK    GeneRIF: Diacylglycerol kinase alpha is a proliferation marker of
            intrahepatic cholangiocarcinoma associated with the prognosis.
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Chen,J., Zhao,D., Zhang,L., Zhang,J., Xiao,Y., Wu,Q., Wang,Y. and
            Zhan,Q.
  TITLE     Tumor-associated macrophage (TAM)-secreted CCL22 confers cisplatin
            resistance of esophageal squamous cell carcinoma (ESCC) cells via
            regulating the activity of diacylglycerol kinase alpha
            (DGKalpha)/NOX4 axis
  JOURNAL   Drug Resist Updat 73, 101055 (2024)
   PUBMED   38387281
  REMARK    GeneRIF: Tumor-associated macrophage (TAM)-secreted CCL22 confers
            cisplatin resistance of esophageal squamous cell carcinoma (ESCC)
            cells via regulating the activity of diacylglycerol kinase alpha
            (DGKalpha)/NOX4 axis.
REFERENCE   3  (bases 1 to 2652)
  AUTHORS   Liu,W., Wang,H., Zhao,Q., Tao,C., Qu,W., Hou,Y., Huang,R., Sun,Z.,
            Zhu,G., Jiang,X., Fang,Y., Gao,J., Wu,X., Yang,Z., Ping,R.,
            Chen,J., Yang,R., Chu,T., Zhou,J., Fan,J., Tang,Z., Yang,D. and
            Shi,Y.
  TITLE     Multiomics analysis reveals metabolic subtypes and identifies
            diacylglycerol kinase alpha (DGKA) as a potential therapeutic
            target for intrahepatic cholangiocarcinoma
  JOURNAL   Cancer Commun (Lond) 44 (2), 226-250 (2024)
   PUBMED   38143235
  REMARK    GeneRIF: Multiomics analysis reveals metabolic subtypes and
            identifies diacylglycerol kinase alpha (DGKA) as a potential
            therapeutic target for intrahepatic cholangiocarcinoma.
REFERENCE   4  (bases 1 to 2652)
  AUTHORS   Zhou,D., Liu,T., Rao,X., Jie,X., Chen,Y., Wu,Z., Deng,H., Zhang,D.,
            Wang,J. and Wu,G.
  TITLE     Targeting diacylglycerol kinase alpha impairs lung tumorigenesis by
            inhibiting cyclin D3
  JOURNAL   Thorac Cancer 14 (13), 1179-1191 (2023)
   PUBMED   36965165
  REMARK    GeneRIF: Targeting diacylglycerol kinase alpha impairs lung
            tumorigenesis by inhibiting cyclin D3.
REFERENCE   5  (bases 1 to 2652)
  AUTHORS   Fu,L., Deng,R., Huang,Y., Yang,X., Jiang,N., Zhou,J., Lin,C.,
            Chen,S., Wu,L., Cui,Q. and Yun,J.
  TITLE     DGKA interacts with SRC/FAK to promote the metastasis of non-small
            cell lung cancer
  JOURNAL   Cancer Lett 532, 215585 (2022)
   PUBMED   35131384
  REMARK    GeneRIF: DGKA interacts with SRC/FAK to promote the metastasis of
            non-small cell lung cancer.
            Erratum:[Cancer Lett. 2022 Jul 28;539:215696. doi:
            10.1016/j.canlet.2022.215696.. PMID: 35477591]
REFERENCE   6  (bases 1 to 2652)
  AUTHORS   Topham,M.K. and Prescott,S.M.
  TITLE     Mammalian diacylglycerol kinases, a family of lipid kinases with
            signaling functions
  JOURNAL   J Biol Chem 274 (17), 11447-11450 (1999)
   PUBMED   10206945
  REMARK    Review article
REFERENCE   7  (bases 1 to 2652)
  AUTHORS   Flores,I., Casaseca,T., Martinez-A,C., Kanoh,H. and Merida,I.
  TITLE     Phosphatidic acid generation through interleukin 2 (IL-2)-induced
            alpha-diacylglycerol kinase activation is an essential step in
            IL-2-mediated lymphocyte proliferation
  JOURNAL   J Biol Chem 271 (17), 10334-10340 (1996)
   PUBMED   8626603
REFERENCE   8  (bases 1 to 2652)
  AUTHORS   Hart,T.C., Zhou,J., Champagne,C., Van Dyke,T.E., Rao,P.N. and
            Pettenati,M.J.
  TITLE     Assignment of the human diacylglycerol kinase gene (DAGK) to
            12q13.3 using fluorescence in situ hybridization analysis
  JOURNAL   Genomics 22 (1), 246-247 (1994)
   PUBMED   7959783
REFERENCE   9  (bases 1 to 2652)
  AUTHORS   Hart,T.C., Champagne,C., Zhou,J. and Van Dyke,T.E.
  TITLE     Assignment of the gene for diacylglycerol kinase (DAGK) to human
            chromosome 12
  JOURNAL   Mamm Genome 5 (2), 123-124 (1994)
   PUBMED   8180475
REFERENCE   10 (bases 1 to 2652)
  AUTHORS   Schaap,D., de Widt,J., van der Wal,J., Vandekerckhove,J., van
            Damme,J., Gussow,D., Ploegh,H.L., van Blitterswijk,W.J. and van der
            Bend,R.L.
  TITLE     Purification, cDNA-cloning and expression of human diacylglycerol
            kinase
  JOURNAL   FEBS Lett 275 (1-2), 151-158 (1990)
   PUBMED   2175712
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025162.48.
            
            Summary: The protein encoded by this gene belongs to the eukaryotic
            diacylglycerol kinase family. It acts as a modulator that competes
            with protein kinase C for the second messenger diacylglycerol in
            intracellular signaling pathways. It also plays an important role
            in the resynthesis of phosphatidylinositols and phosphorylating
            diacylglycerol to phosphatidic acid. Several transcript variants
            encoding different isoforms have been identified for this gene.
            [provided by RefSeq, Apr 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.1754880.1,
                                           SRR14038193.3769830.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               AC025162.48        27262-27444
            184-328             AC025162.48        32523-32667
            329-402             AC025162.48        33117-33190
            403-538             AC025162.48        33508-33643
            539-613             AC025162.48        34078-34152
            614-663             AC025162.48        34611-34660
            664-738             AC025162.48        35015-35089
            739-858             AC025162.48        35286-35405
            859-973             AC025162.48        35515-35629
            974-1062            AC025162.48        36182-36270
            1063-1182           AC025162.48        36414-36533
            1183-1281           AC025162.48        36724-36822
            1282-1365           AC025162.48        36997-37080
            1366-1439           AC025162.48        37352-37425
            1440-1525           AC025162.48        38098-38183
            1526-1615           AC025162.48        38274-38363
            1616-1776           AC025162.48        47723-47883
            1777-1841           AC025162.48        48135-48199
            1842-1932           AC025162.48        48441-48531
            1933-2131           AC025162.48        48834-49032
            2132-2252           AC025162.48        49140-49260
            2253-2313           AC025162.48        49450-49510
            2314-2652           AC025162.48        49785-50123
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..2652
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /note="diacylglycerol kinase alpha"
                     /db_xref="GeneID:1606"
                     /db_xref="HGNC:HGNC:2849"
                     /db_xref="MIM:125855"
     exon            1..183
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            184..328
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..2397
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /EC_number="2.7.1.107"
                     /EC_number="2.7.1.93"
                     /note="isoform f is encoded by transcript variant 22; DAG
                     kinase alpha; diglyceride kinase alpha; 80 kDa
                     diacylglycerol kinase; diacylglycerol kinase, alpha 80kDa"
                     /codon_start=1
                     /product="diacylglycerol kinase alpha isoform f"
                     /protein_id="NP_001400532.1"
                     /db_xref="GeneID:1606"
                     /db_xref="HGNC:HGNC:2849"
                     /db_xref="MIM:125855"
                     /translation="MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQ
                     GDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCY
                     FSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPIL
                     QEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVY
                     CNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGG
                     CESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSI
                     YPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQR
                     LRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGG
                     GYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASI
                     AHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSN
                     LSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDL
                     SDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPC
                     TIKITHKNQMPMLMGPPPRSTNFFGFLS"
     exon            329..402
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            403..538
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            539..613
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            614..663
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            664..738
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            739..858
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            859..973
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1062
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1182
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1281
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1365
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1439
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1525
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1615
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1776
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1841
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..1932
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2131
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2252
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2313
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2314..2652
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2615..2620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /note="hexamer: AATAAA"
     polyA_site      2652
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /note="major polyA site"
ORIGIN      
        1 agttcctgcc agtgagtccc taggcctcca tctctctccc ttgctgtacc accttcacca
       61 ccatccatgc gaccccaaga gccttaatga ctctagaaga gactccaggc aggggaagct
      121 gaaaggacct ttcactccct acttttggcc agggccttct gtgccacctg ccaagaccag
      181 caggcctacc ctctgaagag gtccaagcaa cggaagtact actacgaagc tgcctttctg
      241 gccatccttg agaaaaatag acagatggcc aaggagaggg gcctaataag ccccagtgat
      301 tttgcccagc tgcaaaaata catggaatac tccaccaaaa aggtcagtga tgtcctaaag
      361 ctcttcgagg atggcgagat ggctaaatat gtccaaggag atgccattgg gtacgaggga
      421 ttccagcaat tcctgaaaat ctatctcgaa gtggataatg ttcccagaca cctaagcctg
      481 gcactgtttc aatcctttga gactggtcac tgcttaaatg agacaaatgt gacaaaagat
      541 gtggtgtgtc tcaatgatgt ttcctgctac ttttcccttc tggagggtgg tcggccagaa
      601 gacaagttag aattcacctt caagctgtac gacacggaca gaaatgggat cctggacagc
      661 tcagaagtgg acaaaattat cctacagatg atgcgagtgg ctgaatacct ggattgggat
      721 gtgtctgagc tgaggccgat tcttcaggag atgatgaaag agattgacta tgatggcagt
      781 ggctctgtct ctcaagctga gtgggtccgg gctggggcca ccaccgtgcc actgctagtg
      841 ctgctgggtc tggagatgac tctgaaggac gacggacagc acatgtggag gcccaagagg
      901 ttccccagac cagtctactg caatctgtgc gagtcaagca ttggtcttgg caaacaggga
      961 ctgagctgta acctctgtaa gtacactgtt cacgaccagt gtgccatgaa agccctgcct
     1021 tgtgaagtca gcacctatgc caagtctcgg aaggacattg gtgtccaatc acatgtgtgg
     1081 gtgcgaggag gctgtgagtc cgggcgctgc gaccgctgtc agaaaaagat ccggatctac
     1141 cacagtctga ccgggctgca ttgtgtatgg tgccacctag agatccacga tgactgcctg
     1201 caagcggtgg gccatgagtg tgactgtggg ctgctccggg atcacatcct gcctccatct
     1261 tccatctatc ccagtgtcct ggcctctgga ccggatcgta aaaatagcaa aacaagccag
     1321 aagaccatgg atgatttaaa tttgagcacc tctgaggctc tgcggattga ccctgttcct
     1381 aacacccacc cacttctcgt ctttgtcaat cctaagagtg gcgggaagca ggggcaaagg
     1441 ctccgattat tcaaggatgt tcctgatagc cggattttgg tgtgtggtgg agacggcaca
     1501 gtaggctgga ttctagagac cattgacaaa gctaacttgc cagttttgcc tcctgttgct
     1561 gtgttgcccc tgggtactgg aaatgatctg gctcgatgcc taagatgggg aggaggttat
     1621 gaaggacaga atctggcaaa gatcctcaag gatttagaga tgagtaaagt ggtacatatg
     1681 gatcgatggt ctgtggaggt gatacctcaa caaactgaag aaaaaagtga cccagtcccc
     1741 tttcaaatca tcaataacta cttctctatt ggcgtggatg cctctattgc tcatcgattc
     1801 cacatcatgc gagagaaata tccggagaag ttcaacagca gaatgaagaa caagctatgg
     1861 tacttcgaat ttgccacatc tgaatccatc ttctcaacat gcaaaaagct ggaggagtct
     1921 ttgacagttg agatctgtgg gaaaccgctg gatctgagca acctgtccct agaaggcatc
     1981 gcagtgctaa acatccctag catgcatggt ggctccaacc tctggggtga taccaggaga
     2041 ccccatgggg atatctatgg gatcaaccag gccttaggtg ctacagctaa agtcatcacc
     2101 gaccctgata tcctgaaaac ctgtgtacca gacctaagtg acaagagact ggaagtggtt
     2161 gggctggagg gtgcaattga gatgggccaa atctatacca agctcaagaa tgctggacgt
     2221 cggctggcca agtgctctga gatcaccttc cacaccacaa aaacccttcc catgcaaatt
     2281 gacggagaac cctggatgca gacgccctgt acaatcaaga tcacccacaa gaaccagatg
     2341 cccatgctca tgggcccacc cccccgctcc accaatttct ttggcttctt gagctaaggg
     2401 ggacaccctt ggcctccaag ccagccttga acccacctcc ctgtccctgg actctactcc
     2461 cgaggctctg tacattgctg ccacatactc ctgccagctt gggggagtgt tccttcaccc
     2521 tcacagtatt tattatcctg caccacctca ctgttcccca tgcgcacaca catacacaca
     2581 ccccaaaaca catacattga aagtgcctca tctgaataaa atgacttgtg tttccccttt
     2641 gggatctgct aa
//
