LOCUS       NP_001336                735 aa            linear   PRI 27-APR-2025
DEFINITION  diacylglycerol kinase alpha isoform a [Homo sapiens].
ACCESSION   NP_001336
VERSION     NP_001336.2
DBSOURCE    REFSEQ: accession NM_001345.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 735)
  AUTHORS   Shichi,S., Sugiyama,K., Asahi,Y., Shirakawa,C., Nakamoto,H.,
            Kimura,S., Wakizaka,K., Aiyama,T., Nagatsu,A., Orimo,T.,
            Kakisaka,T. and Taketomi,A.
  TITLE     Diacylglycerol kinase alpha is a proliferation marker of
            intrahepatic cholangiocarcinoma associated with the prognosis
  JOURNAL   Cancer Med 13 (9), e7238 (2024)
   PUBMED   38716625
  REMARK    GeneRIF: Diacylglycerol kinase alpha is a proliferation marker of
            intrahepatic cholangiocarcinoma associated with the prognosis.
REFERENCE   2  (residues 1 to 735)
  AUTHORS   Chen,J., Zhao,D., Zhang,L., Zhang,J., Xiao,Y., Wu,Q., Wang,Y. and
            Zhan,Q.
  TITLE     Tumor-associated macrophage (TAM)-secreted CCL22 confers cisplatin
            resistance of esophageal squamous cell carcinoma (ESCC) cells via
            regulating the activity of diacylglycerol kinase alpha
            (DGKalpha)/NOX4 axis
  JOURNAL   Drug Resist Updat 73, 101055 (2024)
   PUBMED   38387281
  REMARK    GeneRIF: Tumor-associated macrophage (TAM)-secreted CCL22 confers
            cisplatin resistance of esophageal squamous cell carcinoma (ESCC)
            cells via regulating the activity of diacylglycerol kinase alpha
            (DGKalpha)/NOX4 axis.
REFERENCE   3  (residues 1 to 735)
  AUTHORS   Liu,W., Wang,H., Zhao,Q., Tao,C., Qu,W., Hou,Y., Huang,R., Sun,Z.,
            Zhu,G., Jiang,X., Fang,Y., Gao,J., Wu,X., Yang,Z., Ping,R.,
            Chen,J., Yang,R., Chu,T., Zhou,J., Fan,J., Tang,Z., Yang,D. and
            Shi,Y.
  TITLE     Multiomics analysis reveals metabolic subtypes and identifies
            diacylglycerol kinase alpha (DGKA) as a potential therapeutic
            target for intrahepatic cholangiocarcinoma
  JOURNAL   Cancer Commun (Lond) 44 (2), 226-250 (2024)
   PUBMED   38143235
  REMARK    GeneRIF: Multiomics analysis reveals metabolic subtypes and
            identifies diacylglycerol kinase alpha (DGKA) as a potential
            therapeutic target for intrahepatic cholangiocarcinoma.
REFERENCE   4  (residues 1 to 735)
  AUTHORS   Zhou,D., Liu,T., Rao,X., Jie,X., Chen,Y., Wu,Z., Deng,H., Zhang,D.,
            Wang,J. and Wu,G.
  TITLE     Targeting diacylglycerol kinase alpha impairs lung tumorigenesis by
            inhibiting cyclin D3
  JOURNAL   Thorac Cancer 14 (13), 1179-1191 (2023)
   PUBMED   36965165
  REMARK    GeneRIF: Targeting diacylglycerol kinase alpha impairs lung
            tumorigenesis by inhibiting cyclin D3.
REFERENCE   5  (residues 1 to 735)
  AUTHORS   Fu,L., Deng,R., Huang,Y., Yang,X., Jiang,N., Zhou,J., Lin,C.,
            Chen,S., Wu,L., Cui,Q. and Yun,J.
  TITLE     DGKA interacts with SRC/FAK to promote the metastasis of non-small
            cell lung cancer
  JOURNAL   Cancer Lett 532, 215585 (2022)
   PUBMED   35131384
  REMARK    GeneRIF: DGKA interacts with SRC/FAK to promote the metastasis of
            non-small cell lung cancer.
            Erratum:[Cancer Lett. 2022 Jul 28;539:215696. doi:
            10.1016/j.canlet.2022.215696. PMID: 35477591]
REFERENCE   6  (residues 1 to 735)
  AUTHORS   Topham,M.K. and Prescott,S.M.
  TITLE     Mammalian diacylglycerol kinases, a family of lipid kinases with
            signaling functions
  JOURNAL   J Biol Chem 274 (17), 11447-11450 (1999)
   PUBMED   10206945
  REMARK    Review article
REFERENCE   7  (residues 1 to 735)
  AUTHORS   Flores,I., Casaseca,T., Martinez-A,C., Kanoh,H. and Merida,I.
  TITLE     Phosphatidic acid generation through interleukin 2 (IL-2)-induced
            alpha-diacylglycerol kinase activation is an essential step in
            IL-2-mediated lymphocyte proliferation
  JOURNAL   J Biol Chem 271 (17), 10334-10340 (1996)
   PUBMED   8626603
REFERENCE   8  (residues 1 to 735)
  AUTHORS   Hart,T.C., Zhou,J., Champagne,C., Van Dyke,T.E., Rao,P.N. and
            Pettenati,M.J.
  TITLE     Assignment of the human diacylglycerol kinase gene (DAGK) to
            12q13.3 using fluorescence in situ hybridization analysis
  JOURNAL   Genomics 22 (1), 246-247 (1994)
   PUBMED   7959783
REFERENCE   9  (residues 1 to 735)
  AUTHORS   Hart,T.C., Champagne,C., Zhou,J. and Van Dyke,T.E.
  TITLE     Assignment of the gene for diacylglycerol kinase (DAGK) to human
            chromosome 12
  JOURNAL   Mamm Genome 5 (2), 123-124 (1994)
   PUBMED   8180475
REFERENCE   10 (residues 1 to 735)
  AUTHORS   Schaap,D., de Widt,J., van der Wal,J., Vandekerckhove,J., van
            Damme,J., Gussow,D., Ploegh,H.L., van Blitterswijk,W.J. and van der
            Bend,R.L.
  TITLE     Purification, cDNA-cloning and expression of human diacylglycerol
            kinase
  JOURNAL   FEBS Lett 275 (1-2), 151-158 (1990)
   PUBMED   2175712
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM836118.1, BQ943057.1,
            BC031870.2 and BC023523.2.
            
            On Jun 9, 2003 this sequence version replaced NP_001336.1.
            
            Summary: The protein encoded by this gene belongs to the eukaryotic
            diacylglycerol kinase family. It acts as a modulator that competes
            with protein kinase C for the second messenger diacylglycerol in
            intracellular signaling pathways. It also plays an important role
            in the resynthesis of phosphatidylinositols and phosphorylating
            diacylglycerol to phosphatidic acid. Several transcript variants
            encoding different isoforms have been identified for this gene.
            [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (3), as well as variants 1, 2,
            4-6, and 15-17, encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.209972.1,
                                           SRR1803612.235168.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331886.10/ ENSP00000328405.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     Protein         1..735
                     /product="diacylglycerol kinase alpha isoform a"
                     /EC_number="2.7.1.107"
                     /EC_number="2.7.1.93"
                     /note="DAG kinase alpha; diglyceride kinase alpha; 80 kDa
                     diacylglycerol kinase; diacylglycerol kinase, alpha 80kDa"
                     /calculated_mol_wt=82500
     Region          4..110
                     /region_name="DAG_kinase_N"
                     /note="Diacylglycerol kinase N-terminus; pfam14513"
                     /db_xref="CDD:464196"
     Region          114..183
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(123,125,127,134,168,170,172,179)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          201..261
                     /region_name="C1_DGK_typeI_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in type I diacylglycerol kinases; cd20799"
                     /db_xref="CDD:410349"
     Region          270..331
                     /region_name="C1_DGKalpha_rpt2"
                     /note="second protein kinase C conserved region 1 (C1
                     domain) found in diacylglycerol kinase alpha (DAG kinase
                     alpha) and similar proteins; cd20890"
                     /db_xref="CDD:410440"
     Region          378..499
                     /region_name="DAGKc"
                     /note="Diacylglycerol kinase catalytic domain (presumed);
                     smart00046"
                     /db_xref="CDD:214487"
     Site            484
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:19608861; propagated from
                     UniProtKB/Swiss-Prot (P23743.3)"
     Region          520..701
                     /region_name="DAGKa"
                     /note="Diacylglycerol kinase accessory domain (presumed);
                     smart00045"
                     /db_xref="CDD:214486"
     CDS             1..735
                     /gene="DGKA"
                     /gene_synonym="DAGK; DAGK1; DGK-alpha"
                     /coded_by="NM_001345.5:265..2472"
                     /note="isoform a is encoded by transcript variant 3"
                     /db_xref="CCDS:CCDS8896.1"
                     /db_xref="GeneID:1606"
                     /db_xref="HGNC:HGNC:2849"
                     /db_xref="MIM:125855"
ORIGIN      
        1 makerglisp sdfaqlqkym eystkkvsdv lklfedgema kyvqgdaigy egfqqflkiy
       61 levdnvprhl slalfqsfet ghclnetnvt kdvvclndvs cyfslleggr pedkleftfk
      121 lydtdrngil dssevdkiil qmmrvaeyld wdvselrpil qemmkeidyd gsgsvsqaew
      181 vragattvpl lvllglemtl kddgqhmwrp krfprpvycn lcessiglgk qglscnlcky
      241 tvhdqcamka lpcevstyak srkdigvqsh vwvrggcesg rcdrcqkkir iyhsltglhc
      301 vwchleihdd clqavghecd cgllrdhilp pssiypsvla sgpdrknskt sqktmddlnl
      361 stsealridp vpnthpllvf vnpksggkqg qrvlwkfqyi lnprqvfnll kdgpeiglrl
      421 fkdvpdsril vcggdgtvgw iletidkanl pvlppvavlp lgtgndlarc lrwgggyegq
      481 nlakilkdle mskvvhmdrw svevipqqte eksdpvpfqi innyfsigvd asiahrfhim
      541 rekypekfns rmknklwyfe fatsesifst ckkleesltv eicgkpldls nlslegiavl
      601 nipsmhggsn lwgdtrrphg diyginqalg atakvitdpd ilktcvpdls dkrlevvgle
      661 gaiemgqiyt klknagrrla kcseitfhtt ktlpmqidge pwmqtpctik ithknqmpml
      721 mgppprstnf fgfls
//
