LOCUS       NM_032564               2407 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens diacylglycerol O-acyltransferase 2 (DGAT2), transcript
            variant 1, mRNA.
ACCESSION   NM_032564
VERSION     NM_032564.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Guo,Y., Abou Daya,F., Le,H.D., Panda,S. and Melkani,G.C.
  TITLE     Diurnal expression of Dgat2 induced by time-restricted feeding
            maintains cardiac health in the Drosophila model of circadian
            disruption
  JOURNAL   Aging Cell 23 (7), e14169 (2024)
   PUBMED   38616316
  REMARK    GeneRIF: Diurnal expression of Dgat2 induced by time-restricted
            feeding maintains cardiac health in the Drosophila model of
            circadian disruption.
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Deng,B., Kong,W., Shen,X., Han,C., Zhao,Z., Chen,S., Zhou,C. and
            Bae-Jump,V.
  TITLE     The role of DGAT1 and DGAT2 in regulating tumor cell growth and
            their potential clinical implications
  JOURNAL   J Transl Med 22 (1), 290 (2024)
   PUBMED   38500157
  REMARK    GeneRIF: The role of DGAT1 and DGAT2 in regulating tumor cell
            growth and their potential clinical implications.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2407)
  AUTHORS   Irshad,Z., Lund,J., Sillars,A., Lovsletten,N.G., Gharanei,S.,
            Salt,I.P., Freeman,D.J., Gill,J.M.R., Thoresen,G.H., Rustan,A.C.
            and Zammit,V.A.
  TITLE     The roles of DGAT1 and DGAT2 in human myotubes are dependent on
            donor patho-physiological background
  JOURNAL   FASEB J 37 (11), e23209 (2023)
   PUBMED   37779421
  REMARK    GeneRIF: The roles of DGAT1 and DGAT2 in human myotubes are
            dependent on donor patho-physiological background.
REFERENCE   4  (bases 1 to 2407)
  AUTHORS   Selvaraj,R., Zehnder,S.V., Watts,R., Lian,J., Das,C., Nelson,R. and
            Lehner,R.
  TITLE     Preferential lipolysis of DGAT1 over DGAT2 generated
            triacylglycerol in Huh7 hepatocytes
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1868 (10), 159376 (2023)
   PUBMED   37516308
  REMARK    GeneRIF: Preferential lipolysis of DGAT1 over DGAT2 generated
            triacylglycerol in Huh7 hepatocytes.
REFERENCE   5  (bases 1 to 2407)
  AUTHORS   Chun,J., Riella,C.V., Chung,H., Shah,S.S., Wang,M., Magraner,J.M.,
            Ribas,G.T., Ribas,H.T., Zhang,J.Y., Alper,S.L., Friedman,D.J. and
            Pollak,M.R.
  TITLE     DGAT2 Inhibition Potentiates Lipid Droplet Formation To Reduce
            Cytotoxicity in APOL1 Kidney Risk Variants
  JOURNAL   J Am Soc Nephrol 33 (5), 889-907 (2022)
   PUBMED   35232775
  REMARK    GeneRIF: DGAT2 Inhibition Potentiates Lipid Droplet Formation To
            Reduce Cytotoxicity in APOL1 Kidney Risk Variants.
REFERENCE   6  (bases 1 to 2407)
  AUTHORS   Yamada,S., Ohira,M., Horie,H., Ando,K., Takayasu,H., Suzuki,Y.,
            Sugano,S., Hirata,T., Goto,T., Matsunaga,T., Hiyama,E., Hayashi,Y.,
            Ando,H., Suita,S., Kaneko,M., Sasaki,F., Hashizume,K., Ohnuma,N.
            and Nakagawara,A.
  TITLE     Expression profiling and differential screening between
            hepatoblastomas and the corresponding normal livers: identification
            of high expression of the PLK1 oncogene as a poor-prognostic
            indicator of hepatoblastomas
  JOURNAL   Oncogene 23 (35), 5901-5911 (2004)
   PUBMED   15221005
REFERENCE   7  (bases 1 to 2407)
  AUTHORS   Wakimoto,K., Chiba,H., Michibata,H., Seishima,M., Kawasaki,S.,
            Okubo,K., Mitsui,H., Torii,H. and Imai,Y.
  TITLE     A novel diacylglycerol acyltransferase (DGAT2) is decreased in
            human psoriatic skin and increased in diabetic mice
  JOURNAL   Biochem Biophys Res Commun 310 (2), 296-302 (2003)
   PUBMED   14521909
  REMARK    GeneRIF: DGAT2 expression is decreased in psoriatic skin.
REFERENCE   8  (bases 1 to 2407)
  AUTHORS   Winter,A., van Eckeveld,M., Bininda-Emonds,O.R., Habermann,F.A. and
            Fries,R.
  TITLE     Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos
            taurus) and other mammals
  JOURNAL   Cytogenet Genome Res 102 (1-4), 42-47 (2003)
   PUBMED   14970677
REFERENCE   9  (bases 1 to 2407)
  AUTHORS   Cases,S., Stone,S.J., Zhou,P., Yen,E., Tow,B., Lardizabal,K.D.,
            Voelker,T. and Farese,R.V. Jr.
  TITLE     Cloning of DGAT2, a second mammalian diacylglycerol
            acyltransferase, and related family members
  JOURNAL   J Biol Chem 276 (42), 38870-38876 (2001)
   PUBMED   11481335
REFERENCE   10 (bases 1 to 2407)
  AUTHORS   Lehner,R. and Kuksis,A.
  TITLE     Biosynthesis of triacylglycerols
  JOURNAL   Prog Lipid Res 35 (2), 169-201 (1996)
   PUBMED   8944226
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB462004.1 and AL834287.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_032564.4.
            
            Summary: This gene encodes one of two enzymes which catalyzes the
            final reaction in the synthesis of triglycerides in which
            diacylglycerol is covalently bound to long chain fatty acyl-CoAs.
            The encoded protein catalyzes this reaction at low concentrations
            of magnesium chloride while the other enzyme has high activity at
            high concentrations of magnesium chloride. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.311749.1,
                                           SRR1803613.72903.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000228027.12/ ENSP00000228027.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                DB462004.1         46-121
            77-2407             AL834287.1         1-2331
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.5"
     gene            1..2407
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /note="diacylglycerol O-acyltransferase 2"
                     /db_xref="GeneID:84649"
                     /db_xref="HGNC:HGNC:16940"
                     /db_xref="MIM:606983"
     exon            1..335
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     CDS             215..1381
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.76"
                     /note="isoform 1 is encoded by transcript variant 1;
                     diglyceride acyltransferase 2; diacylglycerol
                     O-acyltransferase-like protein 2; diacylglycerol
                     O-acyltransferase homolog 2; acyl-CoA retinol
                     O-fatty-acyltransferase"
                     /codon_start=1
                     /product="diacylglycerol O-acyltransferase 2 isoform 1"
                     /protein_id="NP_115953.2"
                     /db_xref="CCDS:CCDS31642.1"
                     /db_xref="GeneID:84649"
                     /db_xref="HGNC:HGNC:16940"
                     /db_xref="MIM:606983"
                     /translation="MKTLIAAYSGVLRGERQAEADRSQRSHGGPALSREGSGRWGTGS
                     SILSALQDLFSVTWLNRSKVEKQLQVISVLQWVLSFLVLGVACSAILMYIFCTDCWLI
                     AVLYFTWLVFDWNTPKKGGRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGY
                     HPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVSR
                     DTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLRNRKGFVKLALRHGADLVPI
                     YSFGENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPI
                     TTVVGEPITIPKLEHPTQQDIDLYHTMYMEALVKLFDKHKTKFGLPETEVLEVN"
     exon            336..464
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            465..572
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            573..643
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            644..848
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1023
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1226
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..2407
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1484..1489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /note="hexamer: ATTATA"
     polyA_site      1506
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /note="major polyA site"
     regulatory      2385..2390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /note="hexamer: AATAAA"
     polyA_site      2407
                     /gene="DGAT2"
                     /gene_synonym="ARAT; GS1999FULL; HMFN1045"
                     /note="major polyA site"
ORIGIN      
        1 gccgcggctg ccgcctctgc tggggtctag gctgtttctc tcgcgccacc actggccgcc
       61 ggccgcagct ccaggtgtcc tagccgccca gcctcgacgc cgtcccggga cccctgtgct
      121 ctgcgcgaag ccctggcccc gggggccggg gcatgggcca ggggcgcggg gtgaagcggc
      181 ttcccgcggg gccgtgactg ggcgggcttc agccatgaag accctcatag ccgcctactc
      241 cggggtcctg cgcggcgagc gtcaggccga ggctgaccgg agccagcgct ctcacggagg
      301 acctgcgctg tcgcgcgagg ggtctgggag atggggcact ggatccagca tcctctccgc
      361 cctccaggac ctcttctctg tcacctggct caataggtcc aaggtggaaa agcagctaca
      421 ggtcatctca gtgctccagt gggtcctgtc cttccttgta ctgggagtgg cctgcagtgc
      481 catcctcatg tacatattct gcactgattg ctggctcatc gctgtgctct acttcacttg
      541 gctggtgttt gactggaaca cacccaagaa aggtggcagg aggtcacagt gggtccgaaa
      601 ctgggctgtg tggcgctact ttcgagacta ctttcccatc cagctggtga agacacacaa
      661 cctgctgacc accaggaact atatctttgg ataccacccc catggtatca tgggcctggg
      721 tgccttctgc aacttcagca cagaggccac agaagtgagc aagaagttcc caggcatacg
      781 gccttacctg gctacactgg caggcaactt ccgaatgcct gtgttgaggg agtacctgat
      841 gtctggaggt atctgccctg tcagccggga caccatagac tatttgcttt caaagaatgg
      901 gagtggcaat gctatcatca tcgtggtcgg gggtgcggct gagtctctga gctccatgcc
      961 tggcaagaat gcagtcaccc tgcggaaccg caagggcttt gtgaaactgg ccctgcgtca
     1021 tggagctgac ctggttccca tctactcctt tggagagaat gaagtgtaca agcaggtgat
     1081 cttcgaggag ggctcctggg gccgatgggt ccagaagaag ttccagaaat acattggttt
     1141 cgccccatgc atcttccatg gtcgaggcct cttctcctcc gacacctggg ggctggtgcc
     1201 ctactccaag cccatcacca ctgttgtggg agagcccatc accatcccca agctggagca
     1261 cccaacccag caagacatcg acctgtacca caccatgtac atggaggccc tggtgaagct
     1321 cttcgacaag cacaagacca agttcggcct cccggagact gaggtcctgg aggtgaactg
     1381 agccagcctt cggggccaat tccctggagg aaccagctgc aaatcacttt tttgctctgt
     1441 aaatttggaa gtgtcatggg tgtctgtggg ttatttaaaa gaaattataa caattttgct
     1501 aaaccattac aatgttaggt cttttttaag aaggaaaaag tcagtatttc aagttctttc
     1561 acttccagct tgccctgttc taggtggtgg ctaaatctgg gcctaatctg ggtggctcag
     1621 ctaacctctc ttcttccctt cctgaagtga caaaggaaac tcagtcttct tggggaagaa
     1681 ggattgccat tagtgacttg gaccagttag atgattcact ttttgcccct agggatgaga
     1741 ggcgaaagcc acttctcata caagcccctt tattgccact accccacgct cgtctagtcc
     1801 tgaaactgca ggaccagttt ctctgccaag gggaggagtt ggagagcaca gttgccccgt
     1861 tgtgtgaggg cagtagtagg catctggaat gctccagttt gatctccctt ctgccacccc
     1921 tacctcaccc ctagtcactc atatcggagc ctggactggc ctccaggatg aggatggggg
     1981 tggcaatgac accctgcagg ggaaaggact gccccccatg caccattgca gggaggatgc
     2041 cgccaccatg agctaggtgg agtaactggt ttttcttggg tggctgatga catggatgca
     2101 gcacagactc agccttggcc tggagcacat gcttactggt ggcctcagtt taccttcccc
     2161 agatcctaga ttctggatgt gaggaagaga tccctcttca gaaggggcct ggccttctga
     2221 gcagcagatt agttccaaag caggtggccc ccgaacccaa gcctcacttt tctgtgcctt
     2281 cctgaggggg ttgggccggg gaggaaaccc aaccctctcc tgtgtgttct gttatctctt
     2341 gatgagatca ttgcaccatg tcagactttt gtatatgcct tgaaaataaa tgaaagtgag
     2401 aatcctc
//
