LOCUS       NM_001144074            2276 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens DET1 partner of COP1 E3 ubiquitin ligase (DET1),
            transcript variant 2, mRNA.
ACCESSION   NM_001144074
VERSION     NM_001144074.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Xie,Y., Cao,Z., Wong,E.W., Guan,Y., Ma,W., Zhang,J.Q.,
            Walczak,E.G., Murphy,D., Ran,L., Sirota,I., Wang,S., Shukla,S.,
            Gao,D., Knott,S.R., Chang,K., Leu,J., Wongvipat,J., Antonescu,C.R.,
            Hannon,G., Chi,P. and Chen,Y.
  TITLE     COP1/DET1/ETS axis regulates ERK transcriptome and sensitivity to
            MAPK inhibitors
  JOURNAL   J Clin Invest 128 (4), 1442-1457 (2018)
   PUBMED   29360641
  REMARK    GeneRIF: COP1/DET1/ETS axis regulates ERK transcriptome and
            sensitivity to MAPK inhibitors.
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Pick,E., Lau,O.S., Tsuge,T., Menon,S., Tong,Y., Dohmae,N.,
            Plafker,S.M., Deng,X.W. and Wei,N.
  TITLE     Mammalian DET1 regulates Cul4A activity and forms stable complexes
            with E2 ubiquitin-conjugating enzymes
  JOURNAL   Mol Cell Biol 27 (13), 4708-4719 (2007)
   PUBMED   17452440
  REMARK    GeneRIF: These findings demonstrate that the conserved DET1 complex
            modulates Cul4A functions by a novel mechanism.
REFERENCE   3  (bases 1 to 2276)
  AUTHORS   Jin,J., Arias,E.E., Chen,J., Harper,J.W. and Walter,J.C.
  TITLE     A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2,
            which is required for S phase destruction of the replication factor
            Cdt1
  JOURNAL   Mol Cell 23 (5), 709-721 (2006)
   PUBMED   16949367
REFERENCE   4  (bases 1 to 2276)
  AUTHORS   Wertz,I.E., O'Rourke,K.M., Zhang,Z., Dornan,D., Arnott,D.,
            Deshaies,R.J. and Dixit,V.M.
  TITLE     Human De-etiolated-1 regulates c-Jun by assembling a CUL4A
            ubiquitin ligase
  JOURNAL   Science 303 (5662), 1371-1374 (2004)
   PUBMED   14739464
  REMARK    GeneRIF: promotes ubiquitination and degradation of c-Jun by
            assembling a multisubunit ubiquitin ligase containing DNA Damage
            Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1
            (ROC1), and constitutively photomorphogenic-1
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL707845.1, BC109060.1, AK054603.1 and BC001242.2.
            
            On Feb 27, 2020 this sequence version replaced NM_001144074.2.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' region compared to variant 1. The resulting isoform (2) is
            shorter at the N-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB073887.1, SRR14038196.28600.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149398, SAMN03267758
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268148.13/ ENSP00000268148.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AL707845.1         23-141
            120-1889            BC109060.1         1-1770
            1890-2273           AK054603.1         1932-2315
            2274-2276           BC001242.2         1557-1559
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..2276
                     /gene="DET1"
                     /note="DET1 partner of COP1 E3 ubiquitin ligase"
                     /db_xref="GeneID:55070"
                     /db_xref="HGNC:HGNC:25477"
                     /db_xref="MIM:608727"
     exon            1..142
                     /gene="DET1"
                     /inference="alignment:Splign:2.1.0"
     exon            143..1235
                     /gene="DET1"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1805
                     /gene="DET1"
                     /note="isoform 2 is encoded by transcript variant 2; DET1
                     homolog; de-etiolated homolog 1; DET1, COP1 ubiquitin
                     ligase partner"
                     /codon_start=1
                     /product="DET1 homolog isoform 2"
                     /protein_id="NP_001137546.1"
                     /db_xref="CCDS:CCDS45344.1"
                     /db_xref="GeneID:55070"
                     /db_xref="HGNC:HGNC:25477"
                     /db_xref="MIM:608727"
                     /translation="MDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQ
                     NVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEG
                     EILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHLNRECSLFTDDCRCVIVGSAA
                     YLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFKCDKVVLSH
                     NQGLYLYKNILAILSVQQQTIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQ
                     RDSQTGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKM
                     QLLDENHLFIKYTSEDVVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFEN
                     FCDLFRNATLHSEVQFPCSASSNNFARQIQRRFKDTIINAKYGGHTEAVRRLLGQLPI
                     SAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFYARDSGLLKFEIQAGLLGR
                     PINHTVRRLVAFTFHPFEPFAISVQRTNAEYVVNFHMRHCCT"
     exon            1236..1423
                     /gene="DET1"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1615
                     /gene="DET1"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..2276
                     /gene="DET1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2248..2253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DET1"
                     /note="hexamer: AATAAA"
     polyA_site      2276
                     /gene="DET1"
                     /note="major polyA site"
ORIGIN      
        1 agttccggtg gctggcggaa acgggaacgt gcagccgcgg gtgcaggagt cctggggcat
       61 ggcgggggcg gggcaggggg aggcgcgcac agaacaggct ggggcatccc tcgccctggc
      121 tctttgagcc cggaccagac agagatgtga taatggatca tcatgtttct accatcaagc
      181 ctcgaagaat ccaaaaccaa aatgtcattc accgcttgga acgccggcgg atcagttcag
      241 gcaaggcagg tacccactgg caccaagtcc gagtgttcca tcagaatgtc ttccccaact
      301 tcacagttgt caacgttgaa aagcctcctt gtttcttgcg taaattctca cctgatggac
      361 gctactttat tgctttttct tcagaccaga catctcttga aatctatgag taccagggct
      421 gccaggcagc agaggaccta ctgcagggat acgaaggaga aatcctgtcc aatggcaatg
      481 accagcggtc agtgaatatc cggggccggc tctttgaacg cttttttgtc ctgctgcaca
      541 ttaccaatgt tgcggccaat ggtgagcacc tgaaccggga gtgtagtctc ttcactgatg
      601 actgccgctg tgtcatcgtg ggctcagctg cctacctccc agatgagcct caccctccat
      661 tttttgaggt atatcggaac agtgaatcag tgacccccaa cccacggtcc cctctagaag
      721 actattccct ccatatcatt gaccttcaca ccggccgctt atgtgataca cgcacgttca
      781 agtgtgacaa ggtggtcttg tcacacaacc aagggctgta cttgtacaaa aacatcctgg
      841 ccatcttgtc tgtgcaacaa cagaccatcc atgtcttcca ggtgactcct gaaggcactt
      901 tcattgatgt gcggaccatt ggccgctttt gctatgagga tgacctgctc actgtgtcag
      961 ctgttttccc tgaggtacag cgggacagtc agacaggcat ggccaatccc tttagggatc
     1021 ctttcatcaa ttccctcaaa caccggttgc tggtatattt gtggcgccgg gcagaacagg
     1081 atggtagtgc aatggccaag aggcgcttct tccagtattt tgaccaactg cggcagctgc
     1141 gaatgtggaa aatgcagctt ctggatgaaa accacctgtt tatcaagtac actagtgagg
     1201 atgtagtaac actgcgagtc acagatccat cacaggcatc tttctttgtg gtgtacaata
     1261 tggtgacgac agaggtgatt gctgtgtttg agaatacatc agatgagctt ttggagctct
     1321 ttgagaactt ctgtgacctt tttcgtaatg ctaccctgca cagtgaagtt cagtttccct
     1381 gctcagcttc tagcaacaat tttgcaaggc agatccagcg ccggttcaaa gacactatta
     1441 taaatgccaa gtatggaggg cacacagagg cagtacgccg gctgctgggt cagctcccca
     1501 tcagtgctca gtcttacagc ggtagcccct atctggattt gtctctcttc agttatgatg
     1561 acaagtgggt atctgtcatg gagcggccca agacttgtgg agatcaccca atcaggttct
     1621 atgcccggga ctcgggcctg ctcaagtttg agatccaggc ggggttattg ggccgcccca
     1681 tcaaccacac agtgcgacgc cttgttgcct tcacctttca cccttttgag cctttcgcta
     1741 tttctgtgca gaggactaat gctgagtatg ttgtcaactt ccatatgcga cactgctgca
     1801 cgtaggtgcc tcaccagagc cagattatct ggtcttccaa gactttgcca ctcacttatc
     1861 tcagtggact ccaaagcaaa agctcccgac tactagctct gttagttcca gcctgctata
     1921 cctcagatgg gagagagcca gagagaggag tgagggtggc tcaacctaat ggaattttta
     1981 aattgtatac aatactgcta ctgattgtta taatatcctc ttgcgttttc cctgtgggaa
     2041 tgcccagcat taattaagtc catttcattt ttgctttact ttgcatttga ttgctgtgaa
     2101 gatgaaagca ttagactttt atccccttca tgtcacttct tcggcattat ggtttgcatc
     2161 tgaaagcagt taaatcttgt ttactgatga gaatgacata catcctttcc atttagctca
     2221 taagcacggc tatcttttta agagaaaaat aaagccatgg tattttcata cttaaa
//
