LOCUS       NM_001242896            6554 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit
            (DEPDC5), transcript variant 4, mRNA.
ACCESSION   NM_001242896
VERSION     NM_001242896.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Tao,L., Song,P., Shao,L., Gao,H., Ji,K., Ren,Y., Wang,F. and
            Wang,M.
  TITLE     miR-129-2-3p inhibits colon cancer cell proliferation by
            down-regulating the expression of BZW1
  JOURNAL   Arab J Gastroenterol 25 (1), 42-50 (2024)
   PUBMED   38220481
  REMARK    GeneRIF: miR-129-2-3p inhibits colon cancer cell proliferation by
            down-regulating the expression of BZW1.
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   McGinley,C., Teti,S., Hofmann,K., Schreiber,J.M., Cohen,N.T.,
            Gaillard,W.D. and Oluigbo,C.O.
  TITLE     Seizure Control Outcomes following Resection of Cortical Dysplasia
            in Patients with DEPDC5 Variants: A Systematic Review and
            Individual Patient Data Analysis
  JOURNAL   Neuropediatrics 55 (1), 1-8 (2024)
   PUBMED   37984419
  REMARK    GeneRIF: Seizure Control Outcomes following Resection of Cortical
            Dysplasia in Patients with DEPDC5 Variants: A Systematic Review and
            Individual Patient Data Analysis.
REFERENCE   3  (bases 1 to 6554)
  AUTHORS   Wang,H., Liu,W., Zhang,Y., Liu,Q., Cai,L. and Jiang,Y.
  TITLE     Seizure features and outcomes in 50 children with GATOR1 variants:
            A retrospective study, more favorable for epilepsy surgery
  JOURNAL   Epilepsia Open 8 (3), 969-979 (2023)
   PUBMED   37259768
  REMARK    GeneRIF: Seizure features and outcomes in 50 children with GATOR1
            variants: A retrospective study, more favorable for epilepsy
            surgery.
            Review article
REFERENCE   4  (bases 1 to 6554)
  AUTHORS   Ishida,S., Picard,F., Rudolf,G., Noe,E., Achaz,G., Thomas,P.,
            Genton,P., Mundwiller,E., Wolff,M., Marescaux,C., Miles,R.,
            Baulac,M., Hirsch,E., Leguern,E. and Baulac,S.
  TITLE     Mutations of DEPDC5 cause autosomal dominant focal epilepsies
  JOURNAL   Nat Genet 45 (5), 552-555 (2013)
   PUBMED   23542701
  REMARK    GeneRIF: Mutations in DEPDC5 cause familial focal epilepsy with
            variable foci.
REFERENCE   5  (bases 1 to 6554)
  AUTHORS   Dibbens,L.M., de Vries,B., Donatello,S., Heron,S.E., Hodgson,B.L.,
            Chintawar,S., Crompton,D.E., Hughes,J.N., Bellows,S.T., Klein,K.M.,
            Callenbach,P.M., Corbett,M.A., Gardner,A.E., Kivity,S., Iona,X.,
            Regan,B.M., Weller,C.M., Crimmins,D., O'Brien,T.J.,
            Guerrero-Lopez,R., Mulley,J.C., Dubeau,F., Licchetta,L.,
            Bisulli,F., Cossette,P., Thomas,P.Q., Gecz,J., Serratosa,J.,
            Brouwer,O.F., Andermann,F., Andermann,E., van den Maagdenberg,A.M.,
            Pandolfo,M., Berkovic,S.F. and Scheffer,I.E.
  TITLE     Mutations in DEPDC5 cause familial focal epilepsy with variable
            foci
  JOURNAL   Nat Genet 45 (5), 546-551 (2013)
   PUBMED   23542697
  REMARK    GeneRIF: Mutations in DEPDC5 cause familial focal epilepsy with
            variable foci.
REFERENCE   6  (bases 1 to 6554)
  AUTHORS   Miki,D., Ochi,H., Hayes,C.N., Abe,H., Yoshima,T., Aikata,H.,
            Ikeda,K., Kumada,H., Toyota,J., Morizono,T., Tsunoda,T., Kubo,M.,
            Nakamura,Y., Kamatani,N. and Chayama,K.
  TITLE     Variation in the DEPDC5 locus is associated with progression to
            hepatocellular carcinoma in chronic hepatitis C virus carriers
  JOURNAL   Nat Genet 43 (8), 797-800 (2011)
   PUBMED   21725309
  REMARK    GeneRIF: Variation in the DEPDC5 locus is associated with
            progression to hepatocellular carcinoma in chronic hepatitis C
            virus carriers.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6554)
  AUTHORS   Seng,T.J., Ichimura,K., Liu,L., Tingby,O., Pearson,D.M. and
            Collins,V.P.
  TITLE     Complex chromosome 22 rearrangements in astrocytic tumors
            identified using microsatellite and chromosome 22 tile path array
            analysis
  JOURNAL   Genes Chromosomes Cancer 43 (2), 181-193 (2005)
   PUBMED   15770670
REFERENCE   8  (bases 1 to 6554)
  AUTHORS   Xiong,L., Labuda,M., Li,D.S., Hudson,T.J., Desbiens,R., Patry,G.,
            Verret,S., Langevin,P., Mercho,S., Seni,M.H., Scheffer,I.,
            Dubeau,F., Berkovic,S.F., Andermann,F., Andermann,E. and
            Pandolfo,M.
  TITLE     Mapping of a gene determining familial partial epilepsy with
            variable foci to chromosome 22q11-q12
  JOURNAL   Am J Hum Genet 65 (6), 1698-1710 (1999)
   PUBMED   10577924
REFERENCE   9  (bases 1 to 6554)
  AUTHORS   Baulac,S. and Baldassari,S.
  TITLE     DEPDC5-Related Epilepsy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27683934
REFERENCE   10 (bases 1 to 6554)
  AUTHORS   Kurahashi,H. and Hirose,S.
  TITLE     Autosomal Dominant Sleep-Related Hypermotor (Hyperkinetic) Epilepsy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301348
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005004.3, BP354669.1,
            BC136612.1, DB381506.1 and Z83856.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 31, 2019 this sequence version replaced NM_001242896.2.
            
            Summary: This gene encodes a member of the IML1 family of proteins
            involved in G-protein signaling pathways. The mechanistic target of
            rapamycin complex 1 (mTORC1) pathway regulates cell growth by
            sensing the availability of nutrients. The protein encoded by this
            gene is a component of the GATOR1 (GAP activity toward Rags)
            complex which inhibits the amino acid-sensing branch of the mTORC1
            pathway. Mutations in this gene are associated with autosomal
            dominant familial focal epilepsy with variable foci. A single
            nucleotide polymorphism in an intron of this gene has been
            associated with an increased risk of hepatocellular carcinoma in
            individuals with chronic hepatitis C virus infection. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Mar 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC136612.1, SRR1803613.151427.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651528.2/ ENSP00000498382.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC005004.3         47532-47535
            5-34                BP354669.1         1-30
            35-90               BP354669.1         32-87
            91-5289             BC136612.1         1-5199
            5290-5536           DB381506.1         20-266              c
            5537-6554           Z83856.2           9235-10252
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2-q12.3"
     gene            1..6554
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /note="DEP domain containing 5, GATOR1 subcomplex subunit"
                     /db_xref="GeneID:9681"
                     /db_xref="HGNC:HGNC:18423"
                     /db_xref="MIM:614191"
     exon            1..146
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            147..264
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..5018
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /note="isoform 4 is encoded by transcript variant 4; DEP
                     domain-containing protein 5; GATOR complex protein DEPDC5;
                     GATOR1 complex protein DEPDC5"
                     /codon_start=1
                     /product="GATOR1 complex protein DEPDC5 isoform 4"
                     /protein_id="NP_001229825.1"
                     /db_xref="CCDS:CCDS74849.1"
                     /db_xref="GeneID:9681"
                     /db_xref="HGNC:HGNC:18423"
                     /db_xref="MIM:614191"
                     /translation="MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHP
                     NDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVEL
                     TFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDT
                     RVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTV
                     VLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKK
                     LFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSV
                     VITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSR
                     LGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESE
                     NALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPS
                     RTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQ
                     RDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVG
                     PSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSF
                     LNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTV
                     GVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQV
                     FEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSM
                     GRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLE
                     EYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDR
                     PRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTA
                     PPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRK
                     DGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQ
                     NIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYC
                     FISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVT
                     DKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRP
                     ASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNF
                     SLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLR
                     AQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNF
                     PAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNT
                     MLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP"
     exon            265..352
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            353..399
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            400..485
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            486..569
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            570..619
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            620..689
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            690..768
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            769..830
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            831..900
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            901..973
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1077
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1152
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1287
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1349
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1423
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1493
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1530
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1651
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1872
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2076
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2212
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2310
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2376
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2560
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2721
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2839
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..3007
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3008..3227
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3361
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3362..3470
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3471..3536
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3537..3691
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..3769
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3770..3902
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            3903..4011
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4012..4239
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4409
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4410..4581
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4582..4642
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4725
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     exon            4726..6554
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5514..5519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /note="hexamer: AATAAA"
     polyA_site      5536
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /note="major polyA site"
     polyA_site      6368
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
     regulatory      6527..6532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
                     /note="hexamer: TATAAA"
     polyA_site      6554
                     /gene="DEPDC5"
                     /gene_synonym="DEE111; DEP.5; FFEVF; FFEVF1; FPEVF"
ORIGIN      
        1 gggcctagta ggcgcttcag gcttaggggc ggaacagcgc gggccgcgcg ggcgtaggcg
       61 gggtatctgg aggaggcgca gggaacctgg agagggtcca gccctcagtg ccccggccag
      121 aggcgggaag gggctctaga gcttcgggag gcaagatgac ttctctgccc caagcttgga
      181 acagctaaag ggaaaaacag tgcaagatga gaacaacaaa ggtctacaaa ctcgtcatcc
      241 acaagaaggg ctttgggggc agtgatgatg agctagttgt gaaccccaaa gtgttccctc
      301 acatcaagct tggagacatt gtagagattg cacaccccaa cgatgaatac agccctctgc
      361 ttttgcaggt caagtctctt aaggaagatt tacagaagga aactatcagt gtggaccaga
      421 ctgtgactca agtgttccgg ctgagacctt atcaggatgt ctatgttaat gtcgtagacc
      481 ctaaggatgt gacccttgac ctagtggaat taacttttaa ggatcagtat attggccgtg
      541 gggatatgtg gcgactaaag aaaagtttgg tcagcacatg tgcctatatc acccagaagg
      601 tggagtttgc tggcatcaga gcacaggctg gtgaactgtg ggttaagaat gagaaggtca
      661 tgtgtggcta catcagtgaa gataccaggg tggtgtttcg ttctacgtcg gctatggttt
      721 acatatttat tcagatgagc tgtgaaatgt gggattttga tatttatggg gatttgtatt
      781 ttgagaaagc tgtgaatggt ttccttgctg atctatttac caagtggaag gagaagaact
      841 gtagtcatga agtgacagtg gtcctgtttt ctagaacttt ctatgatgca aaatctgttg
      901 atgaatttcc tgaaataaac cgagcctcaa ttcgacagga tcacaagggg agattctatg
      961 aagactttta caaagtggtg gtgcagaatg agagaagaga agaatggact tcacttctcg
     1021 taaccattaa aaaactcttc atccagtatc cagtgttggt gcgactggaa caggcagagg
     1081 gctttcctca aggagataat tctacctcag cacaaggaaa ctacctggag gccatcaatc
     1141 tgtcattcaa tgtgtttgat aagcactaca tcaaccgcaa ctttgaccga actgggcaga
     1201 tgtcagtggt gatcacgccc ggggtgggtg tctttgaagt ggaccgccta ctcatgatcc
     1261 tgaccaagca gcggatgata gataatggaa ttggtgtgga tttggtgtgc atgggagagc
     1321 aaccgttaca tgctgtccca ttgttcaagc tccataatcg gagtgctccc cgtgattctc
     1381 gtctgggcga tgactataat atccctcact ggataaacca cagtttctac acatccaaaa
     1441 gccagctctt ttgtaatagt ttcaccccac gaataaaact ggcaggaaag aagcccgcct
     1501 ctgagaaagc aaaaaatggc cgtgatacat ctctcgggag tccaaaagaa tctgagaacg
     1561 cccttcccat ccaagtagat tatgacgcct atgacgctca agtgttcagg ctgcccggcc
     1621 catcccgggc ccagtgcctc accacctgca gatctgtgcg agagcgagag agtcacagtc
     1681 gaaagagtgc cagctcctgt gatgtttcat ccagcccttc cctaccaagc cgcacactgc
     1741 ccactgagga agtgaggagc caggcttctg acgacagctc cctaggcaag agtgccaaca
     1801 tcctgatgat cccacacccc cacctgcacc agtatgaagt cagcagctcc ttgggataca
     1861 ccagcactcg agatgtcctg gagaacatga tggagccacc acagcgagac tccagtgcac
     1921 cagggaggtt tcacgttggc agtgcagaat ccatgctgca tgttcgacct ggtggataca
     1981 cgccccagag agcactgatt aaccccttcg ctccctctcg gatgcccatg aagcttacgt
     2041 ccaacagaag gcgctggatg cacacttttc ctgtggggcc atccggagaa gccatccaga
     2101 tccaccacca gacccgacag aatatggcgg agctacaagg cagcgggcag agggatccaa
     2161 ctcactcctc tgcagagctg ctggagttag catatcatga agctgctgga aggcacagca
     2221 attcccgcca gcctggtgac ggcatgtcct tcttgaactt cagtggaaca gaggagcttt
     2281 ctgtcggcct gcttagcaac agtggtgcag gtatgaatcc taggacccag aataaggatt
     2341 ctctagagga cagtgtttct acctctccag acccaattct gacactgtct gctccccctg
     2401 tagtgccagg cttctgttgc acagttggag tggactggaa gtctctcact actccggcgt
     2461 gcctccccct taccaccgac tacttccctg accgccaggg cctgcagaat gactacacag
     2521 agggctgtta tgatctcctt ccagaagcag acatcgacag gagggacgaa gatggtgtgc
     2581 agatgacagc ccagcaggta tttgaagagt ttatttgcca acgtctcatg cagggctacc
     2641 aaatcatagt gcagcccaag acacagaaac ccaatcctgc tgtcccgccc ccgctgagca
     2701 gtagcccact ctatagccga ggccttgtgt cccgaaaccg ccctgaggag gaggaccagt
     2761 attggctgag tatgggcaga acgttccaca aagtgacgct gaaggataag atgatcacag
     2821 tgacgcgata ccttcccaag tatccttatg aatctgccca gatccactac acctacagcc
     2881 tctgtccttc ccactcagac tcagagttcg tctcctgctg ggtggaattc tcccacgaac
     2941 ggctggagga gtacaagtgg aattacttag atcagtatat ctgttctgcc ggctctgaag
     3001 acttcagctt aattgagtcc ctgaagttct ggaggacccg cttcctgctg ctgccagcct
     3061 gtgtcaccgc caccaagcgc atcacggagg gggaggccca ctgcgacatc tatggggaca
     3121 ggccccgtgc agacgaggac gagtggcaac tcctggatgg ttttgtccgc tttgtggagg
     3181 gcttgaatcg cattcgcagg cggcatcgct cggatcgcat gatgcggaaa gggaccgcca
     3241 tgaaaggctt gcagatgact gggcccattt ccacgcattc tctggagtca actgcacccc
     3301 cagtggggaa gaagggaacc tcagctctct ctgccctgtt ggagatggag gccagtcaga
     3361 agtgcctggg agaacagcag gcagctgtgc atggtgggaa gagctccgcc cagtcagccg
     3421 agagcagcag cgttgccatg actcccacct acatggacag cccacgaaag gacggggcct
     3481 tctttatgga gtttgtccgc agcccacgca cagcatcgtc cgccttctac cctcaggtat
     3541 ctgtggacca aacagccact cctatgttgg acggcaccag tttgggcata tgcacaggcc
     3601 aatccatgga cagaggcaac agccagacct ttgggaactc ccagaacata ggagaacagg
     3661 gctactcctc cacaaactcc agtgacagca gctctcagca gctggtggca agctccttga
     3721 cctcatcctc taccctgaca gagatcctgg aagccatgaa gcacccctcg acaggagtcc
     3781 agctgctctc tgaacagaag ggcctctcac cgtactgctt catcagcgcg gaggtggtac
     3841 actggttggt gaaccacgtg gaggggatcc agacacaggc gatggccatt gacatcatgc
     3901 agaaaatgct ggaagagcag ctcatcacac atgcatctgg cgaagcctgg cggaccttca
     3961 tctacggctt ctatttctac aagatagtaa cggacaaaga gcccgaccga gtggccatgc
     4021 agcagcccgc caccacctgg cacacagcag gagtggacga cttcgccagc ttccagcgca
     4081 agtggtttga ggtggccttt gtggcagaag agctcgtgca ctctgagatt cctgcctttc
     4141 tcctgccctg gctgcctagc cggccagcct cctatgcaag taggcacagc tcctttagcc
     4201 gaagttttgg aggacggagc caggcggcag cacttttagc tgccactgtc ccagagcaga
     4261 ggactgtgac cctggatgtt gacgtgaaca accgcacaga ccggctggag tggtgcagct
     4321 gttattacca tggcaacttt tctctgaatg cagcctttga gatcaagctg cactggatgg
     4381 cggtgaccgc agcagtactc ttcgagatgg tccaaggttg gcatcggaaa gccacctcct
     4441 gtggcttctt gttagtccca gttttggagg ggccttttgc actgcccagt tacctgtatg
     4501 gcgaccccct tcgtgcccag ctcttcatcc cactcaacat cagctgcttg ctcaaggagg
     4561 gcagcgagca cctgtttgat agctttgaac ccgaaacgta ctgggatcga atgcacctct
     4621 tccaggaagc cattgcacac aggtttgggt ttgtacaaga taaatattct gcctctgctt
     4681 ttaacttccc tgctgagaac aagcctcagt atatccacgt tacaggaaca gtgtttctgc
     4741 agctgcccta ctccaagcgc aagttctcag ggcagcagcg gcggcggcgg aactccacca
     4801 gctccaccaa ccagaacatg ttctgcgagg agcgggtcgg ctacaactgg gcctacaaca
     4861 ccatgctcac caaaacatgg cgctccagcg ccacagggga tgaaaagttt gctgatcggc
     4921 tgctgaagga cttcacggac ttctgcatca accgtgacaa ccggctggtc acgttctgga
     4981 caagttgcct ggagaagatg catgccagtg ccccgtgagg ccaggctgca cctgtgctgg
     5041 gggaaggtgg gtgagccact gccctcaaac ccggggcgga ggattccagg caggctctag
     5101 gagtcaggtg tccgtttgct gctatcagtg agtgggggcc attgtttttt gtttgtttgt
     5161 ttgtttgttt gtttgttttt ggcccccacg acaagtcttc tactctagaa gaaagacttt
     5221 ggaagcagct gctgctgctg ccaccactcc tgtcagcaag tgctcagagc aggtgggagg
     5281 cacagattgt ccgtgggagg gctccagtgt ctgggaagag ggcaggcggc ccccatgaat
     5341 gtcctcggaa gggggtggct cctggtagca tccttttcct tcaccatcta tgggatatta
     5401 ggggcagaat ctgccacttc ttgcccagga gtgtgcacag atgtaagata attttgtgaa
     5461 ataatgtacc atagactctc accaactgta tatacctgta catatcagaa gcaaataaag
     5521 agctccacgt gcatcatttc tttccccacc cagttcccca cagaagcagt cccatcctgg
     5581 gctgggcagt tgtgccaggt ggccggggtc atggcagtca atgaagtggt cacagatggt
     5641 gagagggctt cagaattcac tggcttagag tctggctggg agtttattga gagatttctt
     5701 tcataggcca ctgggcagga gcaaggatgg ttccagtgag gacagtgagc aggcagtata
     5761 acttacaagg taactgcagg ctcatgctgt cagaatgaat cttgctaaat gggtgagctc
     5821 agctctggga cgcagatatg atggcattct gaaaaaaatc aaaggaaaca ttttgtagac
     5881 tctaacttag gttttttttg ttgttgtttt ttttgttgtt ttttttgaca cagttttcac
     5941 tcttgttgcc caggctggag tgcaatggtg tgatctcagc tcactacaac ctctgcctcc
     6001 tgggttcaag cgattctcct gtctcggcct cccgagtagc tgggattaca ggcacctgcc
     6061 accataccag gctaattttt tgtattttaa gtagagatgg ggtttcacca tgttggccag
     6121 gctggtcttg aactcctcac ctcaggcgat ccacccacct cagcctccca aagtgctggg
     6181 attacaggtg tgagccacca tgcccggcct aacttagatt tgctgagata agactcagct
     6241 tggtgcttca cctcagcacc ctgccttaag ctactcaggg cagttcagga gctgtgggtc
     6301 agaggggaga atccgacagt gactgccact tcttgctgtg ccatctcacc acgtggagtt
     6361 catcacaagc tctgttgatt tgcctcctca atggctttgc atccattctc ttcttcccag
     6421 gccctgagct gccactcagt ccagccttcc tttgtctttc tcttagatcc ccaggaaaac
     6481 cttctaattt gtctctgctt tcactccact tgtcagtcag tctttttata aaaccactgt
     6541 gattttgccc acca
//
