LOCUS       NM_003677               2719 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens density regulated re-initiation and release factor
            (DENR), mRNA.
ACCESSION   NM_003677
VERSION     NM_003677.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Grove,D.J., Russell,P.J. and Kearse,M.G.
  TITLE     To initiate or not to initiate: A critical assessment of eIF2A,
            eIF2D, and MCT-1.DENR to deliver initiator tRNA to ribosomes
  JOURNAL   Wiley Interdiscip Rev RNA 15 (2), e1833 (2024)
   PUBMED   38433101
  REMARK    GeneRIF: To initiate or not to initiate: A critical assessment of
            eIF2A, eIF2D, and MCT-1.DENR to deliver initiator tRNA to
            ribosomes.
            Review article
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Clemm von Hohenberg,K., Muller,S., Schleich,S., Meister,M.,
            Bohlen,J., Hofmann,T.G. and Teleman,A.A.
  TITLE     Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote
            mitotic protein translation and faithful cell division
  JOURNAL   Nat Commun 13 (1), 668 (2022)
   PUBMED   35115540
  REMARK    GeneRIF: Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to
            promote mitotic protein translation and faithful cell division.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2719)
  AUTHORS   Petrozziello,T., Bordt,E.A., Mills,A.N., Kim,S.E., Sapp,E.,
            Devlin,B.A., Obeng-Marnu,A.A., Farhan,S.M.K., Amaral,A.C.,
            Dujardin,S., Dooley,P.M., Henstridge,C., Oakley,D.H., Neueder,A.,
            Hyman,B.T., Spires-Jones,T.L., Bilbo,S.D., Vakili,K.,
            Cudkowicz,M.E., Berry,J.D., DiFiglia,M., Silva,M.C., Haggarty,S.J.
            and Sadri-Vakili,G.
  TITLE     Targeting Tau Mitigates Mitochondrial Fragmentation and Oxidative
            Stress in Amyotrophic Lateral Sclerosis
  JOURNAL   Mol Neurobiol 59 (1), 683-702 (2022)
   PUBMED   34757590
REFERENCE   4  (bases 1 to 2719)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2719)
  AUTHORS   Bohlen,J., Harbrecht,L., Blanco,S., Clemm von Hohenberg,K.,
            Fenzl,K., Kramer,G., Bukau,B. and Teleman,A.A.
  TITLE     DENR promotes translation reinitiation via ribosome recycling to
            drive expression of oncogenes including ATF4
  JOURNAL   Nat Commun 11 (1), 4676 (2020)
   PUBMED   32938922
  REMARK    GeneRIF: DENR promotes translation reinitiation via ribosome
            recycling to drive expression of oncogenes including ATF4.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2719)
  AUTHORS   Nakamura,N., Kimura,Y., Tokuda,M., Honda,S. and Hirose,S.
  TITLE     MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to
            change mitochondrial morphology
  JOURNAL   EMBO Rep 7 (10), 1019-1022 (2006)
   PUBMED   16936636
REFERENCE   7  (bases 1 to 2719)
  AUTHORS   Yonashiro,R., Ishido,S., Kyo,S., Fukuda,T., Goto,E., Matsuki,Y.,
            Ohmura-Hoshino,M., Sada,K., Hotta,H., Yamamura,H., Inatome,R. and
            Yanagi,S.
  TITLE     A novel mitochondrial ubiquitin ligase plays a critical role in
            mitochondrial dynamics
  JOURNAL   EMBO J 25 (15), 3618-3626 (2006)
   PUBMED   16874301
REFERENCE   8  (bases 1 to 2719)
  AUTHORS   Stelzl,U., Worm,U., Lalowski,M., Haenig,C., Brembeck,F.H.,
            Goehler,H., Stroedicke,M., Zenkner,M., Schoenherr,A., Koeppen,S.,
            Timm,J., Mintzlaff,S., Abraham,C., Bock,N., Kietzmann,S.,
            Goedde,A., Toksoz,E., Droege,A., Krobitsch,S., Korn,B.,
            Birchmeier,W., Lehrach,H. and Wanker,E.E.
  TITLE     A human protein-protein interaction network: a resource for
            annotating the proteome
  JOURNAL   Cell 122 (6), 957-968 (2005)
   PUBMED   16169070
REFERENCE   9  (bases 1 to 2719)
  AUTHORS   Oh,J.J., Grosshans,D.R., Wong,S.G. and Slamon,D.J.
  TITLE     Identification of differentially expressed genes associated with
            HER-2/neu overexpression in human breast cancer cells
  JOURNAL   Nucleic Acids Res 27 (20), 4008-4017 (1999)
   PUBMED   10497265
REFERENCE   10 (bases 1 to 2719)
  AUTHORS   Deyo,J.E., Chiao,P.J. and Tainsky,M.A.
  TITLE     drp, a novel protein expressed at high cell density but not during
            growth arrest
  JOURNAL   DNA Cell Biol 17 (5), 437-447 (1998)
   PUBMED   9628587
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP872988.1, AB014731.1,
            CB043904.1, AF103800.1, CN300034.1 and AI093455.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003677.4.
            
            Summary: This gene encodes a protein whose expression was found to
            increase in cultured cells at high density but not during growth
            arrest. This gene was also shown to have increased expression in
            cells overexpressing HER-2/neu proto-oncogene. The protein contains
            an SUI1 domain. In budding yeast, SUI1 is a translation initiation
            factor that along with eIF-2 and the initiator tRNA-Met, directs
            the ribosome to the proper translation start site. Proteins similar
            to SUI have been found in mammals, insects, and plants. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.261324.1,
                                           SRR1660807.117039.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280557.11/ ENSP00000280557.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BP872988.1         4-29
            27-907              AB014731.1         1-881
            908-1197            CB043904.1         85-374              c
            1198-1514           AF103800.1         924-1240
            1515-1806           AB014731.1         1566-1857
            1807-1876           CN300034.1         434-503
            1877-2586           AB014731.1         1928-2637
            2587-2719           AI093455.1         10-142              c
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..2719
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /note="density regulated re-initiation and release factor"
                     /db_xref="GeneID:8562"
                     /db_xref="HGNC:HGNC:2769"
                     /db_xref="MIM:604550"
     exon            1..127
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            128..242
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..733
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /note="smooth muscle cell associated protein-3"
                     /codon_start=1
                     /product="density-regulated protein"
                     /protein_id="NP_003668.2"
                     /db_xref="CCDS:CCDS45003.1"
                     /db_xref="GeneID:8562"
                     /db_xref="HGNC:HGNC:2769"
                     /db_xref="MIM:604550"
                     /translation="MAADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYC
                     EYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISEGQGTAGEEEEKKKQKRGGRG
                     QIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEIDLKEAQRFFAQKFSCGASVTG
                     EDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK"
     exon            243..262
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            263..347
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            348..431
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            432..548
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            549..688
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     exon            689..2719
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2697..2702
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /note="hexamer: ATTAAA"
     polyA_site      2719
                     /gene="DENR"
                     /gene_synonym="DRP; DRP1; SMAP-3"
                     /note="major polyA site"
ORIGIN      
        1 gtctcgcggg agcgctgctg gcggtcgggc gctcgggcgg ccctggccgg ggagacgagt
       61 tgcatgtgtt ggttcagctg gcgatagcgg cgggagcgga gccggcgggg cctgtgcgac
      121 cgcctgggtt tgtgaaatgg ctgctgacat ttctgaatcc agcggggctg actgcaaagg
      181 agacccaagg aacagtgcca agttagatgc cgattaccca cttcgagtcc tttattgtgg
      241 agtctgttca ttaccaacag agtactgtga atatatgcct gatgttgcta aatgtagaca
      301 atggttagag aagaattttc caaatgaatt tgcaaaactt actgtagaaa attcacccaa
      361 acaagaagct ggaattagtg agggtcaagg aacagcaggg gaagaagagg agaagaaaaa
      421 acagaagaga ggtggaaggg gtcaaataaa acaaaaaaag aagaccgtac cacaaaaggt
      481 tactatagcc aaaattccca gagcaaagaa gaaatatgtg acaagagtat gtggccttgc
      541 aacttttgaa attgatctta aagaagcaca aagatttttt gctcaaaaat tctcctgtgg
      601 tgcctcagta acaggggagg atgaaattat cattcaggga gattttacag atgacataat
      661 tgatgtcatt caggaaaaat ggccagaggt agatgatgac agcatcgaag atcttggaga
      721 agtaaagaag tgaatttgaa aatttgtctg tatttaatgg cctgaactga gagttgatat
      781 ggccaaaggg agagaggcct tttaaaatat atatatatat acacatatat atgtatatat
      841 acacatatat gtatgtatac acatatacac atgtatatat acatgtgtgt atgtatacat
      901 gtatatatat atacatacac atatatgtat acatatatac acatatatgt atacatatat
      961 acacatatgt atacatatat atatattcta cagtaaaact gtagactgtc ctcgtccttg
     1021 gcattttcac tgttctgtac aaggctgctt gtttttttat tgccaaagtc aaataaacgg
     1081 gagactgtca tgctcatgca tgaatagaat ttagtcaaat aaaaaatttt ggtcatttgg
     1141 tactgacttt ctctctctct ctctctctct tttttttttt tgacagagtc tcgcactgtt
     1201 gcctgggctg gaatgcagtg gtgcgatctc ggctcactgc aacctccgcc tcccgggttc
     1261 aagtgattct cctgcctcag cctcccaagt aggtgggatt acaggcgccc gccaccacgc
     1321 ccagctaatt tttgtatttt tagtagagat ggggtttcac tatgttggcc aggctggtct
     1381 cgaactcctg acctcgtgat tggcccacct cagcctccca aagtgctggt attacagatg
     1441 tgagccaccg cacccagcct gagtttctct ttctctcttt ttaactttat tttttgaaaa
     1501 acccggtaga ctttttgtgg ggagcatttt tgttgataat tttactgatc taaagctgag
     1561 tgatttttta aaagaatttg aatttggctt cctcaccagt aatatgtctc cttgcttctt
     1621 tgatgtgata gttttgagat gggtgagaat ctaatagatc tgtggttgaa tttgctgtgt
     1681 tgttatgaag tccaccctgt gggcacaata acataactgt tggtaggagt tgtttgagct
     1741 attctggaga ttatttggta aagtatacta aaagccttaa aaccatgtat gtgcgctgtt
     1801 tgaacccgta agccacttct ttgacattag aagacattag aagaaataat cagccttgca
     1861 taaaacttat ggatgaaagt attcatcaca atattattta taataaaaaa ttgcaaatgt
     1921 tataaatgaa caattgggaa atggttaaag aagtgatggt gcattgtgtg gtagaatatt
     1981 atgcatatgt ttaaagaatc atattttcta agattatttg gaagcatgtt tggtaatgtc
     2041 aagtggagta ccccagatac attttagaca tttatcgtca tcatctgctc tgagtggaag
     2101 gccgttcaga gaggctagag gttcttattc tggctataaa ttatgtgagt aaaattgtgc
     2161 taaccagtta aaagtactgt acacccatgc tcaatatata gtcctggaaa tagcaattga
     2221 aacatgtctt ctcacaagag aaaatgacag ttttaatgat gtatttgatg aatttaaact
     2281 ttaagtcagg tgctgcaaat tggaaagaag acttgtggtg ttttaagttg ctgtggacac
     2341 ttttaagaaa cttagaaccc atggaaccct tgtttatcgc catgcaaatt acaatcttga
     2401 atgagtgttt tttaaaaata aagtattaga aaaatgtgta gtaaagatgt aaaattaaaa
     2461 aatggaattc tccattaact gtggatttta ctaaatagaa ttactggtga agcagattta
     2521 tccatcgaga ctatctggta tgcgttatgt atgtagtctg ttgctgctga aagatgtctg
     2581 tgtgcctgta tcaacatgtg acttcatgta aagtttcttt gtgttcacag ttcttagcaa
     2641 atgcagttac aatccataga tagccagcag tggatgttac tccaggaaaa tgcaggatta
     2701 aaattgtcct tgtgtataa
//
