LOCUS       NM_015213               4991 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens DENN domain containing 5A (DENND5A), transcript
            variant 1, mRNA.
ACCESSION   NM_015213 NM_001348748 XM_290550
VERSION     NM_015213.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Banks,E., Francis,V., Lin,S.J., Kharfallah,F., Fonov,V.,
            Levesque,M., Han,C., Kulasekaran,G., Tuznik,M., Bayati,A.,
            Al-Khater,R., Alkuraya,F.S., Argyriou,L., Babaei,M., Bahlo,M.,
            Bakhshoodeh,B., Barr,E., Bartik,L., Bassiony,M., Bertrand,M.,
            Braun,D., Buchert,R., Budetta,M., Cadieux-Dion,M., Calame,D.G.,
            Cope,H., Cushing,D., Efthymiou,S., Elmaksoud,M.A., El Said,H.G.,
            Froukh,T., Gill,H.K., Gleeson,J.G., Gogoll,L., Goh,E.S.,
            Gowda,V.K., Haack,T.B., Hashem,M.O., Hauser,S., Hoffman,T.L.,
            Hogue,J.S., Hosokawa,A., Houlden,H., Huang,K., Huynh,S.,
            Karimiani,E.G., Kaulfuss,S., Korenke,G.C., Kritzer,A., Lee,H.,
            Lupski,J.R., Marco,E.J., McWalter,K., Minassian,A., Minassian,B.A.,
            Murphy,D., Neira-Fresneda,J., Northrup,H., Nyaga,D.M.,
            Oehl-Jaschkowitz,B., Osmond,M., Person,R., Pehlivan,D., Petree,C.,
            Sadleir,L.G., Saunders,C., Schoels,L., Shashi,V., Spillmann,R.C.,
            Srinivasan,V.M., Torbati,P.N., Tos,T., Zaki,M.S., Zhou,D.,
            Zweier,C., Trempe,J.F., Durcan,T.M., Gan-Or,Z., Avoli,M., Alves,C.,
            Varshney,G.K., Maroofian,R., Rudko,D.A. and McPherson,P.S.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     Loss of symmetric cell division of apical neural progenitors drives
            DENND5A-related developmental and epileptic encephalopathy
  JOURNAL   Nat Commun 15 (1), 7239 (2024)
   PUBMED   39174524
  REMARK    GeneRIF: Loss of symmetric cell division of apical neural
            progenitors drives DENND5A-related developmental and epileptic
            encephalopathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Yang,M., Johnsson,P., Brautigam,L., Yang,X.R., Thrane,K., Gao,J.,
            Tobin,N.P., Zhou,Y., Yu,R., Nagy,N., Engstrom,P.G., Tuominen,R.,
            Eriksson,H., Lundeberg,J., Tucker,M.A., Goldstein,A.M.,
            Egyhazi-Brage,S., Zhao,J., Cao,Y. and Hoiom,V.
  TITLE     Novel loss-of-function variant in DENND5A impedes melanosomal cargo
            transport and predisposes to familial cutaneous melanoma
  JOURNAL   Genet Med 24 (1), 157-169 (2022)
   PUBMED   34906508
  REMARK    GeneRIF: Novel loss-of-function variant in DENND5A impedes
            melanosomal cargo transport and predisposes to familial cutaneous
            melanoma.
REFERENCE   3  (bases 1 to 4991)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4991)
  AUTHORS   Han,C., Alkhater,R., Froukh,T., Minassian,A.G., Galati,M.,
            Liu,R.H., Fotouhi,M., Sommerfeld,J., Alfrook,A.J., Marshall,C.,
            Walker,S., Bauer,P., Scherer,S.W., Riess,O., Buchert,R.,
            Minassian,B.A. and McPherson,P.S.
  TITLE     Epileptic Encephalopathy Caused by Mutations in the Guanine
            Nucleotide Exchange Factor DENND5A
  JOURNAL   Am J Hum Genet 99 (6), 1359-1367 (2016)
   PUBMED   27866705
  REMARK    GeneRIF: identification of an epileptic encephalopathy additionally
            featuring cerebral calcifications and coarse facial features caused
            by recessive loss-of-function mutations in DENND5A
REFERENCE   5  (bases 1 to 4991)
  AUTHORS   Fernandes,H., Franklin,E., Jollivet,F., Bliedtner,K. and Khan,A.R.
  TITLE     Mapping the interactions between a RUN domain from DENND5/Rab6IP1
            and sorting nexin 1
  JOURNAL   PLoS One 7 (4), e35637 (2012)
   PUBMED   22558185
  REMARK    GeneRIF: A RUN Domain from DENND5/Rab6IP1 binds sorting Nexin 1 and
            is involved in the trafficking of vesicles at the level of Golgi.
REFERENCE   6  (bases 1 to 4991)
  AUTHORS   Yoshimura,S., Gerondopoulos,A., Linford,A., Rigden,D.J. and
            Barr,F.A.
  TITLE     Family-wide characterization of the DENN domain Rab GDP-GTP
            exchange factors
  JOURNAL   J Cell Biol 191 (2), 367-381 (2010)
   PUBMED   20937701
REFERENCE   7  (bases 1 to 4991)
  AUTHORS   Wassmer,T., Attar,N., Harterink,M., van Weering,J.R., Traer,C.J.,
            Oakley,J., Goud,B., Stephens,D.J., Verkade,P., Korswagen,H.C. and
            Cullen,P.J.
  TITLE     The retromer coat complex coordinates endosomal sorting and
            dynein-mediated transport, with carrier recognition by the
            trans-Golgi network
  JOURNAL   Dev Cell 17 (1), 110-122 (2009)
   PUBMED   19619496
REFERENCE   8  (bases 1 to 4991)
  AUTHORS   Recacha,R., Boulet,A., Jollivet,F., Monier,S., Houdusse,A., Goud,B.
            and Khan,A.R.
  TITLE     Structural basis for recruitment of Rab6-interacting protein 1 to
            Golgi via a RUN domain
  JOURNAL   Structure 17 (1), 21-30 (2009)
   PUBMED   19141279
  REMARK    GeneRIF: crystal structure of Rab6a(GTP) in complex with a
            378-residue internal fragment of the effector Rab6IP1 was solved at
            3.2 angstroms resolution. This Rab6IP1 region encompasses an all
            alpha-helical RUN domain followed in tandem by a PLAT domain
REFERENCE   9  (bases 1 to 4991)
  AUTHORS   Janoueix-Lerosey,I., Jollivet,F., Camonis,J., Marche,P.N. and
            Goud,B.
  TITLE     Two-hybrid system screen with the small GTP-binding protein Rab6.
            Identification of a novel mouse GDP dissociation inhibitor isoform
            and two other potential partners of Rab6
  JOURNAL   J Biol Chem 270 (24), 14801-14808 (1995)
   PUBMED   7782346
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC055845.15, DN999250.1,
            AB029014.1 and AL117448.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_015213.3.
            
            Summary: This gene encodes a DENN-domain-containing protein that
            functions as a RAB-activating guanine nucleotide exchange factor
            (GEF). This protein catalyzes the conversion of GDP to GTP and
            thereby converts inactive GDP-bound Rab proteins into their active
            GTP-bound form. The encoded protein is recruited by RAB6 onto Golgi
            membranes and is therefore referred to as RAB6-interacting protein
            1. This protein binds with RAB39 as well. Alternative splicing
            results in multiple transcript variants encoding distinct isoforms.
            Mutations in this gene are associated with early infantile
            epileptic encephalopathy-49. [provided by RefSeq, Feb 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL117448.1, AB029014.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000328194.8/ ENSP00000328524.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC055845.15        145174-145197
            25-64               DN999250.1         8-47
            65-950              AB029014.1         1-886
            951-4991            AL117448.1         683-4723
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..4991
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /note="DENN domain containing 5A"
                     /db_xref="GeneID:23258"
                     /db_xref="HGNC:HGNC:19344"
                     /db_xref="MIM:617278"
     exon            1..390
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             282..4145
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /note="isoform 1 is encoded by transcript variant 1; RAB6
                     interacting protein 1; DENN domain-containing protein 5A;
                     DENN/MADD domain containing 5A; rab6-interacting protein
                     1"
                     /codon_start=1
                     /product="DENN domain-containing protein 5A isoform 1"
                     /protein_id="NP_056028.2"
                     /db_xref="CCDS:CCDS31423.1"
                     /db_xref="GeneID:23258"
                     /db_xref="HGNC:HGNC:19344"
                     /db_xref="MIM:617278"
                     /translation="MSGGGGGGGSAPSRFADYFVICGLDTETGLEPDELSALCQYIQA
                     SKARDGASPFISSTTEGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPK
                     GLAFKTQADPREPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAE
                     YDVLHAPPADDRDQSSMEDGEDTPVTKLQRFNSYDISRDTLYVSKCICLITPMSFMKA
                     CRSVLEQLHQAVTSPQPPPLPLESYIYNVLYEVPLPPPGRSLKFSGVYGPIICQRPST
                     NELPLFDFPVKEVFELLGVENVFQLFTCALLEFQILLYSQHYQRLMTVAETITALMFP
                     FQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFI
                     ELPEDLPQFPNKLEFVQEVSEILMAFGIPPEGNLHCSESASKLKRLRASELVSDKRNG
                     NIAGSPLHSYELLKENETIARLQALVKRTGVSLEKLEVREDPSSNKDLKVQCDEEELR
                     IYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPE
                     PYLPFLSRFLETQMFASFIDNKIMCHDDDDKDPVLRVFDSRVDKIRLLNVRTPTLRTS
                     MYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQSDVLSTGP
                     ASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARTMGSTIRQPKLSNLSP
                     SVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCD
                     LLERIWSHGLQVKQGKSALWSHLLHYQDNRQRKLTSGSLSTSGILLDSERRKSDASSL
                     MPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKL
                     YKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTILIPYHILIVPSKKLGGSMFT
                     ANPWICISGELGETQIMQIPRNVLEMTFECQNLGKLTTVQIGHDNSGLYAKWLVEYVM
                     VRNEITGHTYKFPCGRWLGKGMDDGSLERILVGELLTSQPEVDERPCRTPPLQQSPSV
                     IRRLVTISPNNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSA
                     LEQAFQHGFKSPRLFKNVFIWDFLEKAQTYYETLEKNEVVPEENWHTRARNFCRFVTA
                     INNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAHMYEDVALIKDHTLVNS
                     LIRVLQTLQEFNITLETSLVKGIDI"
     exon            391..462
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            463..572
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..1230
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1418
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1736
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1952
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2187
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2338
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2432
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2564
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2717
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2802
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2887
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2888..3016
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3138
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3284
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3285..3403
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3585
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3586..3668
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3669..3792
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3793..3961
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3962..4991
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4970..4975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /note="hexamer: AATAAA"
     polyA_site      4991
                     /gene="DENND5A"
                     /gene_synonym="DEE49; EIEE49; RAB6IP1"
                     /note="major polyA site"
ORIGIN      
        1 cttctccagg ctcgctcggg ccgcggcgct ctcggctagc tgcagcggcg gcggcgcgga
       61 ggggaaggca tccaggatgc ggtgcggggc ggcccggtgc ccccccgccc cgtcacggca
      121 gccgcggcgg ccgaggggac cgggccaggg ccgggggcgg cggcccgagc cgcggtagcg
      181 gcggcggcgg gaggggcggc ctgagggcgg acgggcgggc gcccgggttg cgggggctcg
      241 gtgccgctcc gcactgcccg gccggtctcg gccccggcgc catgagtggc ggcggcggcg
      301 gagggggctc ggcgcccagt cgcttcgccg actactttgt catctgcgga ctggacacgg
      361 agaccgggct ggagccggac gagctgtcgg cattatgcca gtacatacag gcttctaaag
      421 ccagggatgg tgccagccct ttcatttcaa gtacgactga aggagaaaat tttgagcaga
      481 caccattgag aagaacattc aaatctaagg tccttgcacg atatcctgag aacgtagaat
      541 ggaatccctt tgaccaagat gcagtaggaa tgctatgtat gccgaaaggg ctggcattca
      601 agacccaggc tgatcccagg gagccccaat tccatgcctt tattatcaca agggaggatg
      661 gctctcggac atttgggttt gccctcacat tttatgaaga ggtgactagc aagcagatct
      721 gcagtgcaat gcagaccctc taccacatgc acaatgctga gtatgatgtc ctacatgctc
      781 cccctgctga tgacagagac cagagcagca tggaggatgg tgaagacact cctgtgacca
      841 aactgcagcg cttcaactcc tatgacatta gccgggacac tctctacgtc tctaagtgca
      901 tctgcctcat cacacccatg tctttcatga aggcatgtcg gagcgtgctg gagcaactcc
      961 accaggcagt cacttcacct cagccccctc cactgcccct tgagagctac atatacaacg
     1021 tactctacga ggtgccgctc ccacctcctg gccggtcctt gaagttttct ggggtctatg
     1081 ggccaataat ctgccagaga ccaagtacca atgagcttcc cctatttgac tttcctgtca
     1141 aagaggtttt tgaactgctc ggggtggaga atgtgtttca gctttttact tgtgcccttc
     1201 tggagtttca aatcctgctc tactcacagc attaccagag actgatgact gtggcggaga
     1261 cgattacagc tctcatgttt cctttccagt ggcagcatgt ctatgtccct attctcccag
     1321 cttctctcct gcatttctta gatgctcctg ttccatacct gatgggtttg cattccaatg
     1381 gcctggatga ccggtcaaag ctggagctgc ctcaagaggc taacctctgc tttgtggaca
     1441 ttgacaacca cttcattgag ttgccagagg acttgccaca gttccccaac aaattggagt
     1501 ttgtccagga agtctctgag attctcatgg catttggaat tccccctgaa gggaatcttc
     1561 attgcagtga gagtgcctcc aagctgaaga ggctgcgggc ctctgagctt gtctcggaca
     1621 agaggaatgg gaacattgct ggctcccctt tgcattccta cgagcttctt aaggagaatg
     1681 aaactattgc ccggctgcaa gccttggtca agagaactgg ggtgagcctg gaaaagttgg
     1741 aagtgcgtga agaccccagc agcaataagg atctcaaagt tcagtgtgat gaagaagaac
     1801 tcaggattta ccagctaaac attcagatcc gggaagtttt tgcaaatcgt ttcactcaga
     1861 tgtttgcaga ttatgaggtg tttgtcatcc aacccagcca ggataaggaa tcctggttta
     1921 ccaacaggga gcaaatgcaa aactttgata aagcatcttt tctgtcagat cagcctgagc
     1981 cctacctgcc cttcctctca agattcctgg agacccagat gtttgcatct ttcattgaca
     2041 acaaaataat gtgtcatgat gatgatgata aagaccctgt actccgggta tttgattccc
     2101 gagttgacaa gatcaggctg ttgaatgttc ggacacctac tctccgtaca tccatgtacc
     2161 agaagtgtac cactgtggat gaagcagaga aagcaattga gctgcgtctg gcaaaaattg
     2221 accatactgc aattcaccca catttacttg acatgaagat tggacaaggg aaatatgagc
     2281 cgggcttctt ccctaagctg cagtctgatg tactttccac tgggccagcc agcaacaagt
     2341 ggacgaaaag gaatgcccct gcccagtgga ggcggaaaga tcggcagaag cagcacacag
     2401 aacacctgcg tttagataat gaccagaggg agaagtacat ccaggaagcc aggactatgg
     2461 gcagcactat ccgccagccc aaactgtcca acctctctcc atcagtgatt gcccagacca
     2521 attggaagtt tgtagagggc ctgctgaagg aatgccgcaa taagaccaag aggatgctgg
     2581 tggaaaagat gggccgagaa gctgtggagc tagggcatgg ggaggtgaac atcacagggg
     2641 tggaagagaa caccctgatt gccagccttt gtgatctcct ggaaaggatc tggagtcatg
     2701 gactacaagt gaaacagggg aaatcagcct tatggtccca cctgttacat tatcaggaca
     2761 accggcagag aaaactcaca tcaggaagcc tcagtacctc aggaatactt cttgattcag
     2821 aacgtaggaa gtctgatgcc agctcactca tgcctcccct gaggatctcc ctgattcagg
     2881 atatgaggca catccagaac atcggggaaa tcaagactga tgtgggaaag gccagagcat
     2941 gggtgcgact gtccatggaa aaaaagttac tttccagaca cctgaagcag ctcctctcag
     3001 accatgagct caccaaaaag ttatataagc gctatgcctt cctgcgctgt gatgacgaga
     3061 aggagcagtt cctctatcac ctcctgtctt tcaatgccgt cgattacttt tgcttcacca
     3121 atgtcttcac aactatcctg atcccgtacc acattctgat cgtaccaagc aagaagctgg
     3181 ggggctccat gttcactgcc aacccatgga tctgtatatc aggagaattg ggtgagacac
     3241 agatcatgca gattcccagg aatgtgctag agatgacctt cgagtgccag aacttgggga
     3301 agcttactac tgtccagatt ggccatgata actctgggct gtatgccaaa tggctggtgg
     3361 agtatgtgat ggtcaggaat gagatcacag gacataccta caagttcccg tgtggccggt
     3421 ggttagggaa gggcatggat gatggaagcc tggagcggat cctagttggg gagctgctca
     3481 catcccagcc tgaggtggat gagaggccat gccggacccc gccgctgcag cagtccccca
     3541 gtgtcatccg gaggcttgtt accatctcac ccaacaacaa gcccaagctg aacactgggc
     3601 agatccagga gtccatcggg gaggcagtca atggcattgt gaagcacttc cataagcctg
     3661 agaaagagcg aggcagtctg acgctgttgc tctgtggaga gtgtggcctt gtctcggcct
     3721 tggaacaggc tttccagcat ggatttaaat cgccccggct cttcaaaaat gtcttcattt
     3781 gggatttcct ggaaaaagca caaacctatt atgagacatt agagaagaat gaagtagtcc
     3841 ctgaggaaaa ctggcataca agagcccgga acttctgccg atttgtcact gcaatcaaca
     3901 atactccccg gaacatcggc aaggatggca agtttcagat gctggtgtgc ttgggagcca
     3961 gagatcacct cctacaccac tggattgccc tgctggctga ctgccccatc actgcacaca
     4021 tgtatgagga tgtggcactg atcaaagacc atacacttgt caattccttg attcgtgtgc
     4081 tgcagacatt gcaggagttc aacatcacgc tggagacgtc ccttgtcaag ggcatcgaca
     4141 tctgacctcc cagcaccagc cagcagcagg actgagaaag actcaccctg cagctctgac
     4201 cttttttccc aaagggactt aagcgattgt gcaggagtag gagacaaaat gtacactcac
     4261 tgtaaaaaga aaactagagg atttttggaa taaataatct attttagagt ttatttgctg
     4321 atttgctttt tacacacttt catgtgaaag agtgataggg agagggagcg aggctggtgc
     4381 cgcttatttt gaagctggtg ccctccctcg ccgtggccac atgctggaag cctgaggcct
     4441 ccctggactg agcctgtggc actgcgtgcg ggacagttat gtttccttgc cccgtcgcat
     4501 taatgaggcc cttccacatc atttttaaac taatgttttt ctatattaac attattatgg
     4561 atatttggct ttcataggcc acacacaggt gtgctgcgcg ggaagcccca tgctccaatc
     4621 aaagggattt ttagtagtgc ctctaagcaa gcaccgatga gtcagtccca cgtattttct
     4681 tttttgtcag tattgtttgg gaaggagaca tgccgggatg tgtcatcgtg ccaaatacca
     4741 catttcctgt tggcacagtt tcacagaagt aaacataagc atgttttaac aggtttttct
     4801 tttctttttt cttttttaaa atgttttatt tatttaaccc gccattgtgt gtttttaagt
     4861 attttctttt tttaaggaaa ggaaaagctt gtcacaatct aactggctat gttattatta
     4921 ttaaatttat gttttgcaac ttagaaacca gctacagtat ggcccactta ataaaacacc
     4981 tgaaacaaaa a
//
