LOCUS       NM_213618               4341 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens DENN domain containing 2B (DENND2B), transcript
            variant 3, mRNA.
ACCESSION   NM_213618
VERSION     NM_213618.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Kumar,R., Francis,V., Ioannou,M.S., Aguila,A., Khan,M., Banks,E.,
            Kulasekaran,G. and McPherson,P.S.
  TITLE     DENND2B activates Rab35 at the intercellular bridge, regulating
            cytokinetic abscission and tetraploidy
  JOURNAL   Cell Rep 42 (7), 112795 (2023)
   PUBMED   37454296
  REMARK    GeneRIF: DENND2B activates Rab35 at the intercellular bridge,
            regulating cytokinetic abscission and tetraploidy.
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4341)
  AUTHORS   Kim,M.K., Kim,B., Kwon,J.O., Song,M.K., Jung,S., Lee,Z.H. and
            Kim,H.H.
  TITLE     ST5 Positively Regulates Osteoclastogenesis via Src/Syk/calcium
            Signaling Pathways
  JOURNAL   Mol Cells 42 (11), 810-819 (2019)
   PUBMED   31707778
  REMARK    GeneRIF: Findings provide evidence of suppression of tumorigenicity
            5 (ST5) involvement in positive regulation of osteoclastogenesis
            via src Kinases (Src)/Syk Kinase (Syk)/calcium signaling.
REFERENCE   4  (bases 1 to 4341)
  AUTHORS   Ioannou,M.S., Kulasekaran,G., Fotouhi,M., Morein,J.J., Han,C.,
            Tse,S., Nossova,N., Han,T., Mannard,E. and McPherson,P.S.
  TITLE     Intersectin-s interaction with DENND2B facilitates recycling of
            epidermal growth factor receptor
  JOURNAL   EMBO Rep 18 (12), 2119-2130 (2017)
   PUBMED   29030480
  REMARK    GeneRIF: Here, we discover that intersectin-s binds DENND2B, a
            guanine nucleotide exchange factor for the exocytic GTPase Rab13,
            and this interaction promotes recycling of ligand-free EGFR to the
            cell surface.  Our study thus reveals a novel mechanism controlling
            the fate of internalized EGFR with important implications for
            cancer.
REFERENCE   5  (bases 1 to 4341)
  AUTHORS   Ioannou,M.S., Bell,E.S., Girard,M., Chaineau,M., Hamlin,J.N.,
            Daubaras,M., Monast,A., Park,M., Hodgson,L. and McPherson,P.S.
  TITLE     DENND2B activates Rab13 at the leading edge of migrating cells and
            promotes metastatic behavior
  JOURNAL   J Cell Biol 208 (5), 629-648 (2015)
   PUBMED   25713415
  REMARK    GeneRIF: DENND2B interacts with the Rab13 effector MICAL-L2 at the
            cell periphery, and this interaction is required for the dynamic
            remodeling of the cell's leading edge.
REFERENCE   6  (bases 1 to 4341)
  AUTHORS   Majidi,M., Gutkind,J.S. and Lichy,J.H.
  TITLE     Deletion of the COOH terminus converts the ST5 p70 protein from an
            inhibitor of RAS signaling to an activator with transforming
            activity in NIH-3T3 cells
  JOURNAL   J Biol Chem 275 (9), 6560-6565 (2000)
   PUBMED   10692462
REFERENCE   7  (bases 1 to 4341)
  AUTHORS   Hubbs,A.E., Majidi,M. and Lichy,J.H.
  TITLE     Expression of an isoform of the novel signal transduction protein
            ST5 is linked to cell morphology
  JOURNAL   Oncogene 18 (15), 2519-2525 (1999)
   PUBMED   10229203
REFERENCE   8  (bases 1 to 4341)
  AUTHORS   Majidi,M., Hubbs,A.E. and Lichy,J.H.
  TITLE     Activation of extracellular signal-regulated kinase 2 by a novel
            Abl-binding protein, ST5
  JOURNAL   J Biol Chem 273 (26), 16608-16614 (1998)
   PUBMED   9632734
REFERENCE   9  (bases 1 to 4341)
  AUTHORS   Lichy,J.H., Majidi,M., Elbaum,J. and Tsai,M.M.
  TITLE     Differential expression of the human ST5 gene in HeLa-fibroblast
            hybrid cell lines mediated by YY1: evidence that YY1 plays a part
            in tumor suppression
  JOURNAL   Nucleic Acids Res 24 (23), 4700-4708 (1996)
   PUBMED   8972856
REFERENCE   10 (bases 1 to 4341)
  AUTHORS   Lichy,J.H., Modi,W.S., Seuanez,H.N. and Howley,P.M.
  TITLE     Identification of a human chromosome 11 gene which is
            differentially regulated in tumorigenic and nontumorigenic somatic
            cell hybrids of HeLa cells
  JOURNAL   Cell Growth Differ 3 (8), 541-548 (1992)
   PUBMED   1390339
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX647431.1, BC036655.1,
            U15131.1 and U15780.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_213618.1.
            
            Summary: This gene was identified by its ability to suppress the
            tumorigenicity of Hela cells in nude mice. The protein encoded by
            this gene contains a C-terminal region that shares similarity with
            the Rab 3 family of small GTP binding proteins. This protein
            preferentially binds to the SH3 domain of c-Abl kinase, and acts as
            a regulator of MAPK1/ERK2 kinase, which may contribute to its
            ability to reduce the tumorigenic phenotype in cells. Three
            alternatively spliced transcript variants of this gene encoding
            distinct isoforms are identified. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647431.1, AK312305.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000313726.11/ ENSP00000319678.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-421               BX647431.1         4-424
            422-1130            BC036655.1         630-1338
            1131-1379           U15131.1           1062-1310
            1380-2041           BC036655.1         1588-2249
            2042-3960           U15780.1           713-2631
            3961-4341           BX647431.1         3964-4344
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..4341
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="DENN domain containing 2B"
                     /db_xref="GeneID:6764"
                     /db_xref="HGNC:HGNC:11350"
                     /db_xref="MIM:140750"
     exon            1..158
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            159..263
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..3597
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="isoform 1 is encoded by transcript variant 3;
                     DENN/MADD domain containing 2B; suppression of
                     tumorigenicity 5 protein; heLa tumor suppression 1"
                     /codon_start=1
                     /product="DENN domain-containing protein 2B isoform 1"
                     /protein_id="NP_998783.1"
                     /db_xref="CCDS:CCDS7791.1"
                     /db_xref="GeneID:6764"
                     /db_xref="HGNC:HGNC:11350"
                     /db_xref="MIM:140750"
                     /translation="MTMTANKNSSITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPI
                     YPLSDSETSACRYPSHSSSRVLLKDRHPPAPSPQNPQDPSPDTSPPTCPFKTASFGYL
                     DRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPFPGPAAGPRGVLLTRTGTRAH
                     SLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVVPSPCSSEK
                     TFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRK
                     ESSAVLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRKTGTLGSLEEPAGGASVSA
                     GSRAVGVAGVAGEAGPPPEREGSGSTKPGTPGNSPSSQRLPSKSSLDPAVNPVPKPKR
                     TFEYEADKNPKSKPSNGLPPSPTPAAPPPLPSTPAPPVTRRPKKDMRGHRKSQSRKSF
                     EFEDASSLQSLYPSSPTENGTENQPKFGSKSTLEENAYEDIVGDLPKENPYEDVDLKS
                     RRAGRKSQQLSENSLDSLHRMWSPQDRKYNSPPTQLSLKPNSQSLRSGNWSERKSHRL
                     PRLPKRHSHDDMLLLAQLSLPSSPSSLNEDSLSTTSELLSSRRARRIPKLVQRINSIY
                     NAKRGKKRLKKLSMSSIETASLRDENSESESDSDDRFKAHTQRLVHIQSMLKRAPSYR
                     TLELELLEWQERELFEYFVVVSLKKKPSRNTYLPEVSYQFPKLDRPTKQMREAEERLK
                     AIPQFCFPDAKDWLPVSEYSSETFSFMLTGEDGSRRFGYCRRLLPSGKGPRLPEVYCV
                     ISRLGCFGLFSKVLDEVERRRGISAALVYPFMRSLMESPFPAPGKTIKVKTFLPGAGN
                     EVLELRRPMDSRLEHVDFECLFTCLSVRQLIRIFASLLLERRVIFVADKLSTLSSCSH
                     AVVALLYPFSWQHTFIPVLPASMIDIVCCPTPFLVGLLSSSLPKLKELPVEEALMVNL
                     GSDRFIRQMDDEDTLLPRKLQAALEQALERKNELISQDSDSDSDDECNTLNGLVSEVF
                     IRFFVETVGHYSLFLTQSEKGERAFQREAFRKSVASKSIRRFLEVFMESQMFAGFIQD
                     RELRKCRAKGLFEQRVEQYLEELPDTEQSGMNKFLRGLGNKMKFLHKKN"
     exon            264..1523
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1660
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1812
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..2028
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2125
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2170
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2355
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2465
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2535
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2613
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2754
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2903
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3081
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3123
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3235
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3236..3475
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3476..3562
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3563..4341
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4311..4316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="hexamer: AATAAA"
     polyA_site      4341
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="major polyA site"
ORIGIN      
        1 cacttggctg cgaggagcgc cacgaaaggt cagaggaagg agctgtggga agctcgcagc
       61 aggtatcgga gcttaagcca gtggatttgg gggccctggg ctccctagcc ggctgcggtg
      121 tgagaatgga gtgggcagga aagcagcggg actttcaggg ctctgggttt gcagagagcc
      181 gaaatgacca tgactgccaa caagaattcc agcatcaccc acggagctgg tggcactaaa
      241 gcccctcggg ggactctgag caggtctcag tcagtctctc cacctccagt tctctcccca
      301 ccaaggagtc ccatctaccc gctcagtgat agtgaaacct cagcctgcag gtaccccagc
      361 cactccagct cccgggtgct cctcaaggac cggcaccccc cagctccttc accccagaat
      421 cctcaagatc cctccccaga tacttcccca cccacctgtc ccttcaagac cgccagcttc
      481 ggttatttgg acagaagccc ttcggcgtgc aagagagacg cccaaaagga aagtgtccaa
      541 ggcgcagccc aggatgtagc aggggtcgct gcctgcctcc cccttgccca gagcacgcca
      601 ttcccggggc cagcagctgg cccccggggc gtcttgctga cccgtaccgg tacccgcgcc
      661 cacagcctgg gcatccggga gaagatatca gcatgggaag gtcgccgaga ggcgtcgccc
      721 aggatgagca tgtgtggaga gaagcgggag ggctctggga gcgagtgggc ggccagtgag
      781 ggctgcccca gcctgggctg tcccagcgtg gtgccgtccc cctgcagctc tgaaaagacc
      841 tttgatttca agggcctccg gaggatgagc aggaccttct ccgagtgttc ctacccagag
      901 actgaggagg agggagaggc gctccctgtc cgggactctt tctaccggct ggagaaacgg
      961 ctgggccgga gtgagcccag cgccttcctc agggggcatg gcagcaggaa ggagagctca
     1021 gcagtgctga gccggatcca gaaaattgaa caggtcctga aggagcagcc gggccggggg
     1081 ctcccccagc tccccagcag ctgctacagc gtggaccggg ggaaaaggaa gactggaacc
     1141 ttgggctcct tggaggagcc ggcagggggc gcgagtgtga gcgctggcag ccgggcagtc
     1201 ggagtggctg gtgttgcggg ggaggcgggc ccacccccag agagggaagg cagtggttcc
     1261 actaagcccg ggacccctgg aaatagccct agctcccagc ggctgccatc gaagagttcc
     1321 ctcgatcccg ctgtgaaccc tgtccccaaa cccaagcgca cctttgaata cgaggctgac
     1381 aagaacccca agagtaagcc cagtaatggt ctacctcctt cacccacacc tgctgctcca
     1441 cctcccttgc cctccacccc agccccgcca gtcacccgga gacccaagaa ggacatgcgt
     1501 ggtcaccgca agtcccagag cagaaaatcc tttgagtttg aggatgcatc cagtctccag
     1561 tccctgtacc cctcttctcc cactgagaat ggtactgaga accaacccaa gtttggatcc
     1621 aaaagcactt tagaagaaaa tgcctatgaa gatattgtgg gagatctgcc caaggagaat
     1681 ccatatgagg atgtggactt aaagagccga agagcaggac gaaaatccca gcaactgtct
     1741 gagaactcct tggactcttt gcacaggatg tggagtcctc aggacaggaa gtacaacagc
     1801 ccgcccacac agctttccct gaaacccaac agccagtccc tgcgcagtgg gaactggtca
     1861 gaaaggaaga gccaccggct gccacgatta cccaagaggc acagccatga cgacatgctg
     1921 ctgctggctc agctgagtct gccgtcctca ccctccagcc tcaatgaaga cagcctcagc
     1981 accaccagcg agctgctgtc cagccgccgg gcccgccgca ttcccaagct tgtccaaaga
     2041 attaactcca tctacaatgc caagagagga aagaagagat taaaaaagtt gtctatgtcc
     2101 agcattgaaa cagcatcact gagagatgaa aacagtgaga gcgagagcga ctctgatgac
     2161 aggttcaaag cccacacaca gcgcctggtc cacatccagt cgatgctgaa gcgcgccccc
     2221 agctatcgca cgctggagct ggagctgctg gagtggcagg agcgggagct ttttgagtac
     2281 tttgtggtgg tgtccctcaa gaagaagcca tcgcgaaaca cctacctccc cgaagtctcc
     2341 taccagtttc ccaagctgga ccgacccacc aagcagatgc gagaggcaga ggaaaggctc
     2401 aaagccattc cccagttttg cttccctgat gccaaggact ggcttcctgt gtcagagtat
     2461 agcagtgaga ccttttcttt catgctgact ggggaagatg gcagcagacg ctttggctac
     2521 tgcaggcgct tactgccaag tgggaaaggg ccccggttgc cagaggtgta ctgtgtcatc
     2581 agccgccttg gctgcttcgg cttgttttcc aaggtcctag atgaggtgga gcgccggcgt
     2641 gggatctccg ctgcattggt ctatcctttc atgagaagtc tcatggagtc gcccttccca
     2701 gccccaggga agaccatcaa agtgaagaca ttcctgccag gtgctggcaa tgaggtgtta
     2761 gagctgcggc ggcccatgga ctcaaggctg gagcacgtgg actttgagtg cctttttacc
     2821 tgcctcagtg tgcgccagct catccgaatc tttgcctcac tgctgctgga gcgccgggtc
     2881 atttttgtgg cagataagct cagtaccctc tccagctgct cccacgcggt ggtggccttg
     2941 ctctacccct tctcctggca gcacaccttc attcctgtcc tcccggcctc catgattgac
     3001 atcgtctgct gtcccacccc cttcctggtt ggcctgctct ccagctccct ccccaaactg
     3061 aaggagctgc ctgtggagga ggcgctgatg gtgaatctgg gatctgaccg attcatccga
     3121 cagatggacg acgaagacac gttgttacct aggaagttac aggcagctct ggagcaggct
     3181 ctggagagga agaatgagct gatctcccag gactctgaca gcgactccga cgatgaatgt
     3241 aataccctca atgggctggt gtcggaggtg tttatccggt tctttgtgga gaccgttggg
     3301 cactactccc tctttctgac acagagtgag aagggagaga gggcctttca gcgagaggcc
     3361 ttccgcaaat ctgtggcctc caaaagcatc cgccgctttc ttgaggtttt tatggagtct
     3421 cagatgtttg ctggcttcat ccaagacagg gagctaagaa agtgtcgggc aaagggcctt
     3481 tttgagcagc gagtggagca gtacttagaa gaactcccag acactgagca gagtggaatg
     3541 aataagtttc tccgaggttt gggcaacaaa atgaagtttc tccacaagaa gaattaagcc
     3601 tccttctcag tagcagagtc cagtgccttg cagagcctga agcctgggga gaaggcccag
     3661 cctgggaccc tctgggctgc tgtggctcct ctgcccccac agatcctatc ctccaagcca
     3721 gcccacctct gccttcatca tatcccagga tactgtttgt aaataatctg ctgtaagctt
     3781 tcttaactgt tttttgtaac aagcaaagag aatatggcaa atatttgtat attcccaagg
     3841 ggccgggtgc tttcctgtcc tgccagagca tggatgaagt ttcgctgggt gctcgtgact
     3901 ggccagtttt gtgcagctga ctgtctcagc caaaccactg atcttccctg gaggccttcg
     3961 gcctgcctgc ctgcctgagg tccccgctgc cagtcccggg ccctggagag cagatgctgt
     4021 cttgttatgt acaggaggac cttttaaaaa aatcaagttt ctattttttg ctggtagtcc
     4081 gcatacccat accctctgtt tttgaaaggc aaaggccaat cagtccccat ttgtagcatg
     4141 gcaccagggt cttaggccta gtcctctcat tcctcccacc ctccgagatg gtcagtgtgt
     4201 catgggaagc ccacccccag ctctgccagt gctctctggg cctggctccc agtcagtggt
     4261 ggccacgatg cggtacaggg catccctcct tcccatctac gggtgttctc aataaacaat
     4321 gtacagttgt ttgggcccag a
//
