LOCUS       NM_005418               4544 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens DENN domain containing 2B (DENND2B), transcript
            variant 1, mRNA.
ACCESSION   NM_005418
VERSION     NM_005418.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Kumar,R., Francis,V., Ioannou,M.S., Aguila,A., Khan,M., Banks,E.,
            Kulasekaran,G. and McPherson,P.S.
  TITLE     DENND2B activates Rab35 at the intercellular bridge, regulating
            cytokinetic abscission and tetraploidy
  JOURNAL   Cell Rep 42 (7), 112795 (2023)
   PUBMED   37454296
  REMARK    GeneRIF: DENND2B activates Rab35 at the intercellular bridge,
            regulating cytokinetic abscission and tetraploidy.
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4544)
  AUTHORS   Kim,M.K., Kim,B., Kwon,J.O., Song,M.K., Jung,S., Lee,Z.H. and
            Kim,H.H.
  TITLE     ST5 Positively Regulates Osteoclastogenesis via Src/Syk/calcium
            Signaling Pathways
  JOURNAL   Mol Cells 42 (11), 810-819 (2019)
   PUBMED   31707778
  REMARK    GeneRIF: Findings provide evidence of suppression of tumorigenicity
            5 (ST5) involvement in positive regulation of osteoclastogenesis
            via src Kinases (Src)/Syk Kinase (Syk)/calcium signaling.
REFERENCE   4  (bases 1 to 4544)
  AUTHORS   Ioannou,M.S., Kulasekaran,G., Fotouhi,M., Morein,J.J., Han,C.,
            Tse,S., Nossova,N., Han,T., Mannard,E. and McPherson,P.S.
  TITLE     Intersectin-s interaction with DENND2B facilitates recycling of
            epidermal growth factor receptor
  JOURNAL   EMBO Rep 18 (12), 2119-2130 (2017)
   PUBMED   29030480
  REMARK    GeneRIF: Here, we discover that intersectin-s binds DENND2B, a
            guanine nucleotide exchange factor for the exocytic GTPase Rab13,
            and this interaction promotes recycling of ligand-free EGFR to the
            cell surface.  Our study thus reveals a novel mechanism controlling
            the fate of internalized EGFR with important implications for
            cancer.
REFERENCE   5  (bases 1 to 4544)
  AUTHORS   Ioannou,M.S., Bell,E.S., Girard,M., Chaineau,M., Hamlin,J.N.,
            Daubaras,M., Monast,A., Park,M., Hodgson,L. and McPherson,P.S.
  TITLE     DENND2B activates Rab13 at the leading edge of migrating cells and
            promotes metastatic behavior
  JOURNAL   J Cell Biol 208 (5), 629-648 (2015)
   PUBMED   25713415
  REMARK    GeneRIF: DENND2B interacts with the Rab13 effector MICAL-L2 at the
            cell periphery, and this interaction is required for the dynamic
            remodeling of the cell's leading edge.
REFERENCE   6  (bases 1 to 4544)
  AUTHORS   Majidi,M., Gutkind,J.S. and Lichy,J.H.
  TITLE     Deletion of the COOH terminus converts the ST5 p70 protein from an
            inhibitor of RAS signaling to an activator with transforming
            activity in NIH-3T3 cells
  JOURNAL   J Biol Chem 275 (9), 6560-6565 (2000)
   PUBMED   10692462
REFERENCE   7  (bases 1 to 4544)
  AUTHORS   Hubbs,A.E., Majidi,M. and Lichy,J.H.
  TITLE     Expression of an isoform of the novel signal transduction protein
            ST5 is linked to cell morphology
  JOURNAL   Oncogene 18 (15), 2519-2525 (1999)
   PUBMED   10229203
REFERENCE   8  (bases 1 to 4544)
  AUTHORS   Majidi,M., Hubbs,A.E. and Lichy,J.H.
  TITLE     Activation of extracellular signal-regulated kinase 2 by a novel
            Abl-binding protein, ST5
  JOURNAL   J Biol Chem 273 (26), 16608-16614 (1998)
   PUBMED   9632734
REFERENCE   9  (bases 1 to 4544)
  AUTHORS   Lichy,J.H., Majidi,M., Elbaum,J. and Tsai,M.M.
  TITLE     Differential expression of the human ST5 gene in HeLa-fibroblast
            hybrid cell lines mediated by YY1: evidence that YY1 plays a part
            in tumor suppression
  JOURNAL   Nucleic Acids Res 24 (23), 4700-4708 (1996)
   PUBMED   8972856
REFERENCE   10 (bases 1 to 4544)
  AUTHORS   Lichy,J.H., Modi,W.S., Seuanez,H.N. and Howley,P.M.
  TITLE     Identification of a human chromosome 11 gene which is
            differentially regulated in tumorigenic and nontumorigenic somatic
            cell hybrids of HeLa cells
  JOURNAL   Cell Growth Differ 3 (8), 541-548 (1992)
   PUBMED   1390339
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026894.7 and AC091053.11.
            
            On Nov 16, 2019 this sequence version replaced NM_005418.3.
            
            Summary: This gene was identified by its ability to suppress the
            tumorigenicity of Hela cells in nude mice. The protein encoded by
            this gene contains a C-terminal region that shares similarity with
            the Rab 3 family of small GTP binding proteins. This protein
            preferentially binds to the SH3 domain of c-Abl kinase, and acts as
            a regulator of MAPK1/ERK2 kinase, which may contribute to its
            ability to reduce the tumorigenic phenotype in cells. Three
            alternatively spliced transcript variants of this gene encoding
            distinct isoforms are identified. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036655.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               AC026894.7         77076-77206
            132-231             AC026894.7         106918-107017
            232-272             AC026894.7         148677-148717
            273-361             AC026894.7         176692-176780
            362-466             AC091053.11        66849-66953
            467-1726            AC091053.11        86365-87624
            1727-1863           AC091053.11        91365-91501
            1864-2015           AC091053.11        99682-99833
            2016-2231           AC091053.11        101756-101971
            2232-2328           AC091053.11        102868-102964
            2329-2373           AC091053.11        103532-103576
            2374-2558           AC091053.11        104839-105023
            2559-2668           AC091053.11        106343-106452
            2669-2738           AC091053.11        106660-106729
            2739-2816           AC091053.11        109720-109797
            2817-2957           AC091053.11        110349-110489
            2958-3106           AC091053.11        114854-115002
            3107-3284           AC091053.11        118184-118361
            3285-3326           AC091053.11        118600-118641
            3327-3438           AC091053.11        119938-120049
            3439-3678           AC091053.11        120908-121147
            3679-3765           AC091053.11        122025-122111
            3766-4544           AC091053.11        123444-124222
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..4544
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="DENN domain containing 2B"
                     /db_xref="GeneID:6764"
                     /db_xref="HGNC:HGNC:11350"
                     /db_xref="MIM:140750"
     exon            1..131
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            132..231
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            232..272
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            273..361
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            362..466
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     CDS             387..3800
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DENN/MADD domain containing 2B; suppression of
                     tumorigenicity 5 protein; heLa tumor suppression 1"
                     /codon_start=1
                     /product="DENN domain-containing protein 2B isoform 1"
                     /protein_id="NP_005409.3"
                     /db_xref="CCDS:CCDS7791.1"
                     /db_xref="GeneID:6764"
                     /db_xref="HGNC:HGNC:11350"
                     /db_xref="MIM:140750"
                     /translation="MTMTANKNSSITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPI
                     YPLSDSETSACRYPSHSSSRVLLKDRHPPAPSPQNPQDPSPDTSPPTCPFKTASFGYL
                     DRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPFPGPAAGPRGVLLTRTGTRAH
                     SLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVVPSPCSSEK
                     TFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRK
                     ESSAVLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRKTGTLGSLEEPAGGASVSA
                     GSRAVGVAGVAGEAGPPPEREGSGSTKPGTPGNSPSSQRLPSKSSLDPAVNPVPKPKR
                     TFEYEADKNPKSKPSNGLPPSPTPAAPPPLPSTPAPPVTRRPKKDMRGHRKSQSRKSF
                     EFEDASSLQSLYPSSPTENGTENQPKFGSKSTLEENAYEDIVGDLPKENPYEDVDLKS
                     RRAGRKSQQLSENSLDSLHRMWSPQDRKYNSPPTQLSLKPNSQSLRSGNWSERKSHRL
                     PRLPKRHSHDDMLLLAQLSLPSSPSSLNEDSLSTTSELLSSRRARRIPKLVQRINSIY
                     NAKRGKKRLKKLSMSSIETASLRDENSESESDSDDRFKAHTQRLVHIQSMLKRAPSYR
                     TLELELLEWQERELFEYFVVVSLKKKPSRNTYLPEVSYQFPKLDRPTKQMREAEERLK
                     AIPQFCFPDAKDWLPVSEYSSETFSFMLTGEDGSRRFGYCRRLLPSGKGPRLPEVYCV
                     ISRLGCFGLFSKVLDEVERRRGISAALVYPFMRSLMESPFPAPGKTIKVKTFLPGAGN
                     EVLELRRPMDSRLEHVDFECLFTCLSVRQLIRIFASLLLERRVIFVADKLSTLSSCSH
                     AVVALLYPFSWQHTFIPVLPASMIDIVCCPTPFLVGLLSSSLPKLKELPVEEALMVNL
                     GSDRFIRQMDDEDTLLPRKLQAALEQALERKNELISQDSDSDSDDECNTLNGLVSEVF
                     IRFFVETVGHYSLFLTQSEKGERAFQREAFRKSVASKSIRRFLEVFMESQMFAGFIQD
                     RELRKCRAKGLFEQRVEQYLEELPDTEQSGMNKFLRGLGNKMKFLHKKN"
     exon            467..1726
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1863
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..2015
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2231
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2328
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2373
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2558
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2668
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2738
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2816
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2817..2957
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3106
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3284
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3285..3326
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3438
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3678
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3679..3765
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     exon            3766..4544
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4514..4519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="hexamer: AATAAA"
     polyA_site      4544
                     /gene="DENND2B"
                     /gene_synonym="HTS1; p126; ST5"
                     /note="major polyA site"
ORIGIN      
        1 gggggtcggg ggtcgggggc tgggggctga ggactgagtc gagccagcag cccagggcat
       61 ctgtccacgc tgtcagagga gcctctgggc gtggggctgg cccttagagg gctactgcgc
      121 tgcttaactt gcctttcaca gaaaaccact ggaggagata ctcacaaaaa tgaaggggaa
      181 gaacaggaaa caggaagaca tgagatgaag gaaacaggag attcaacatg gatttcacac
      241 cctttggaga gtttctttct tggataattc aggaagatga gagactgctt aggcgccacc
      301 actagtacca tgagtccctg cactggttaa agccatcgcc acaacctgga caggcagcaa
      361 gggctctggg tttgcagaga gccgaaatga ccatgactgc caacaagaat tccagcatca
      421 cccacggagc tggtggcact aaagcccctc gggggactct gagcaggtct cagtcagtct
      481 ctccacctcc agttctctcc ccaccaagga gtcccatcta cccgctcagt gatagtgaaa
      541 cctcagcctg caggtacccc agccactcca gctcccgggt gctcctcaag gaccggcacc
      601 ccccagctcc ttcaccccag aatcctcaag atccctcccc agatacttcc ccacccacct
      661 gtcccttcaa gaccgccagc ttcggttatt tggacagaag cccttcggcg tgcaagagag
      721 acgcccaaaa ggaaagtgtc caaggcgcag cccaggatgt agcaggggtc gctgcctgcc
      781 tcccccttgc ccagagcacg ccattcccgg ggccagcagc tggcccccgg ggcgtcttgc
      841 tgacccgtac cggtacccgc gcccacagcc tgggcatccg ggagaagata tcagcatggg
      901 aaggtcgccg agaggcgtcg cccaggatga gcatgtgtgg agagaagcgg gagggctctg
      961 ggagcgagtg ggcggccagt gagggctgcc ccagcctggg ctgtcccagc gtggtgccgt
     1021 ccccctgcag ctctgaaaag acctttgatt tcaagggcct ccggaggatg agcaggacct
     1081 tctccgagtg ttcctaccca gagactgagg aggagggaga ggcgctccct gtccgggact
     1141 ctttctaccg gctggagaaa cggctgggcc ggagtgagcc cagcgccttc ctcagggggc
     1201 atggcagcag gaaggagagc tcagcagtgc tgagccggat ccagaaaatt gaacaggtcc
     1261 tgaaggagca gccgggccgg gggctccccc agctccccag cagctgctac agcgtggacc
     1321 gggggaaaag gaagactgga accttgggct ccttggagga gccggcaggg ggcgcgagtg
     1381 tgagcgctgg cagccgggca gtcggagtgg ctggtgttgc gggggaggcg ggcccacccc
     1441 cagagaggga aggcagtggt tccactaagc ccgggacccc tggaaatagc cctagctccc
     1501 agcggctgcc atcgaagagt tccctcgatc ccgctgtgaa ccctgtcccc aaacccaagc
     1561 gcacctttga atacgaggct gacaagaacc ccaagagtaa gcccagtaat ggtctacctc
     1621 cttcacccac acctgctgct ccacctccct tgccctccac cccagccccg ccagtcaccc
     1681 ggagacccaa gaaggacatg cgtggtcacc gcaagtccca gagcagaaaa tcctttgagt
     1741 ttgaggatgc atccagtctc cagtccctgt acccctcttc tcccactgag aatggtactg
     1801 agaaccaacc caagtttgga tccaaaagca ctttagaaga aaatgcctat gaagatattg
     1861 tgggagatct gcccaaggag aatccatatg aggatgtgga cttaaagagc cgaagagcag
     1921 gacgaaaatc ccagcaactg tctgagaact ccttggactc tttgcacagg atgtggagtc
     1981 ctcaggacag gaagtacaac agcccgccca cacagctttc cctgaaaccc aacagccagt
     2041 ccctgcgcag tgggaactgg tcagaaagga agagccaccg gctgccacga ttacccaaga
     2101 ggcacagcca tgacgacatg ctgctgctgg ctcagctgag tctgccgtcc tcaccctcca
     2161 gcctcaatga agacagcctc agcaccacca gcgagctgct gtccagccgc cgggcccgcc
     2221 gcattcccaa gcttgtccaa agaattaact ccatctacaa tgccaagaga ggaaagaaga
     2281 gattaaaaaa gttgtctatg tccagcattg aaacagcatc actgagagat gaaaacagtg
     2341 agagcgagag cgactctgat gacaggttca aagcccacac acagcgcctg gtccacatcc
     2401 agtcgatgct gaagcgcgcc cccagctatc gcacgctgga gctggagctg ctggagtggc
     2461 aggagcggga gctttttgag tactttgtgg tggtgtccct caagaagaag ccatcgcgaa
     2521 acacctacct ccccgaagtc tcctaccagt ttcccaagct ggaccgaccc accaagcaga
     2581 tgcgagaggc agaggaaagg ctcaaagcca ttccccagtt ttgcttccct gatgccaagg
     2641 actggcttcc tgtgtcagag tatagcagtg agaccttttc tttcatgctg actggggaag
     2701 atggcagcag acgctttggc tactgcaggc gcttactgcc aagtgggaaa gggccccggt
     2761 tgccagaggt gtactgtgtc atcagccgcc ttggctgctt cggcttgttt tccaaggtcc
     2821 tagatgaggt ggagcgccgg cgtgggatct ccgctgcatt ggtctatcct ttcatgagaa
     2881 gtctcatgga gtcgcccttc ccagccccag ggaagaccat caaagtgaag acattcctgc
     2941 caggtgctgg caatgaggtg ttagagctgc ggcggcccat ggactcaagg ctggagcacg
     3001 tggactttga gtgccttttt acctgcctca gtgtgcgcca gctcatccga atctttgcct
     3061 cactgctgct ggagcgccgg gtcatttttg tggcagataa gctcagtacc ctctccagct
     3121 gctcccacgc ggtggtggcc ttgctctacc ccttctcctg gcagcacacc ttcattcctg
     3181 tcctcccggc ctccatgatt gacatcgtct gctgtcccac ccccttcctg gttggcctgc
     3241 tctccagctc cctccccaaa ctgaaggagc tgcctgtgga ggaggcgctg atggtgaatc
     3301 tgggatctga ccgattcatc cgacagatgg acgacgaaga cacgttgtta cctaggaagt
     3361 tacaggcagc tctggagcag gctctggaga ggaagaatga gctgatctcc caggactctg
     3421 acagcgactc cgacgatgaa tgtaataccc tcaatgggct ggtgtcggag gtgtttatcc
     3481 ggttctttgt ggagaccgtt gggcactact ccctctttct gacacagagt gagaagggag
     3541 agagggcctt tcagcgagag gccttccgca aatctgtggc ctccaaaagc atccgccgct
     3601 ttcttgaggt ttttatggag tctcagatgt ttgctggctt catccaagac agggagctaa
     3661 gaaagtgtcg ggcaaagggc ctttttgagc agcgagtgga gcagtactta gaagaactcc
     3721 cagacactga gcagagtgga atgaataagt ttctccgagg tttgggcaac aaaatgaagt
     3781 ttctccacaa gaagaattaa gcctccttct cagtagcaga gtccagtgcc ttgcagagcc
     3841 tgaagcctgg ggagaaggcc cagcctggga ccctctgggc tgctgtggct cctctgcccc
     3901 cacagatcct atcctccaag ccagcccacc tctgccttca tcatatccca ggatactgtt
     3961 tgtaaataat ctgctgtaag ctttcttaac tgttttttgt aacaagcaaa gagaatatgg
     4021 caaatatttg tatattccca aggggccggg tgctttcctg tcctgccaga gcatggatga
     4081 agtttcgctg ggtgctcgtg actggccagt tttgtgcagc tgactgtctc agccaaacca
     4141 ctgatcttcc ctggaggcct tcggcctgcc tgcctgcctg aggtccccgc tgccagtccc
     4201 gggccctgga gagcagatgc tgtcttgtta tgtacaggag gaccttttaa aaaaatcaag
     4261 tttctatttt ttgctggtag tccgcatacc cataccctct gtttttgaaa ggcaaaggcc
     4321 aatcagtccc catttgtagc atggcaccag ggtcttaggc ctagtcctct cattcctccc
     4381 accctccgag atggtcagtg tgtcatggga agcccacccc cagctctgcc agtgctctct
     4441 gggcctggct cccagtcagt ggtggccacg atgcggtaca gggcatccct ccttcccatc
     4501 tacgggtgtt ctcaataaac aatgtacagt tgtttgggcc caga
//
