LOCUS       NM_017631               6015 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens DExD/H-box helicase 60 (DDX60), transcript variant 1,
            mRNA.
ACCESSION   NM_017631
VERSION     NM_017631.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Zhou,Y., Zhang,Y., Li,M., Ming,T., Zhang,C., Huang,C., Li,J.,
            Li,F., Li,H., Zhao,E., Shu,F., Liu,L., Pan,X., Gao,Y., Tian,L.,
            Song,L., Huang,H. and Liao,W.
  TITLE     Oncogenic KRAS drives immunosuppression of colorectal cancer by
            impairing DDX60-mediated dsRNA accumulation and viral mimicry
  JOURNAL   Sci Immunol 9 (100), eado8758 (2024)
   PUBMED   39365875
  REMARK    GeneRIF: Oncogenic KRAS drives immunosuppression of colorectal
            cancer by impairing DDX60-mediated dsRNA accumulation and viral
            mimicry.
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Nakamura,Y., Ishibashi,H.K., Saruga,T., Imaizumi,T., Kurose,A.,
            Tachizaki,M., Kawaguchi,S., Seya,K., Sasaki,E. and Ishibashi,Y.
  TITLE     Possible involvement of DExD/H box helicase 60 in synovial
            inflammation of rheumatoid arthritis: role of toll-like receptor 3
            signaling
  JOURNAL   Mol Biol Rep 51 (1), 131 (2024)
   PUBMED   38236450
  REMARK    GeneRIF: Possible involvement of DExD/H box helicase 60 in synovial
            inflammation of rheumatoid arthritis: role of toll-like receptor 3
            signaling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6015)
  AUTHORS   Han,Y., Gao,J. and Liu,L.
  TITLE     DDX60 Promotes Migration and Invasion of Head and Neck Squamous
            Cell Carcinoma Cell through the NF-kappaB/IFI27 Signaling Pathway
  JOURNAL   Front Biosci (Landmark Ed) 29 (1), 14 (2024)
   PUBMED   38287816
  REMARK    GeneRIF: DDX60 Promotes Migration and Invasion of Head and Neck
            Squamous Cell Carcinoma Cell through the NF-kappaB/IFI27 Signaling
            Pathway.
REFERENCE   4  (bases 1 to 6015)
  AUTHORS   Chen,W., Li,Z.Y., Huang,L., Zhou,D.H., Luo,W.Q., Zhang,X.F., Li,L.,
            Wen,C.P. and Wang,Q.
  TITLE     Integrative Bioinformatics Analysis Identifies DDX60 as a Potential
            Biomarker for Systemic Lupus Erythematosus
  JOURNAL   Dis Markers 2023, 8564650 (2023)
   PUBMED   36655136
  REMARK    GeneRIF: Integrative Bioinformatics Analysis Identifies DDX60 as a
            Potential Biomarker for Systemic Lupus Erythematosus.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6015)
  AUTHORS   Karasawa,T., Sato,R., Imaizumi,T., Hashimoto,S., Fujita,M.,
            Aizawa,T., Tsugawa,K., Kawaguchi,S., Seya,K., Terui,K. and
            Tanaka,H.
  TITLE     Glomerular endothelial expression of type I IFN-stimulated gene,
            DExD/H-Box helicase 60 via toll-like receptor 3 signaling: possible
            involvement in the pathogenesis of lupus nephritis
  JOURNAL   Ren Fail 44 (1), 137-145 (2022)
   PUBMED   35392757
  REMARK    GeneRIF: Glomerular endothelial expression of type I IFN-stimulated
            gene, DExD/H-Box helicase 60 via toll-like receptor 3 signaling:
            possible involvement in the pathogenesis of lupus nephritis.
REFERENCE   6  (bases 1 to 6015)
  AUTHORS   Hubel,P., Urban,C., Bergant,V., Schneider,W.M., Knauer,B.,
            Stukalov,A., Scaturro,P., Mann,A., Brunotte,L., Hoffmann,H.H.,
            Schoggins,J.W., Schwemmle,M., Mann,M., Rice,C.M. and Pichlmair,A.
  TITLE     A protein-interaction network of interferon-stimulated genes
            extends the innate immune system landscape
  JOURNAL   Nat Immunol 20 (4), 493-502 (2019)
   PUBMED   30833792
REFERENCE   7  (bases 1 to 6015)
  AUTHORS   Fu,T.Y., Wu,C.N., Sie,H.C., Cheng,J.T., Lin,Y.S., Liou,H.H.,
            Tseng,Y.K., Shu,C.W., Tsai,K.W., Yen,L.M., Tseng,H.W., Tseng,C.J.,
            Ger,L.P. and Liu,P.F.
  TITLE     Subsite-specific association of DEAD box RNA helicase DDX60 with
            the development and prognosis of oral squamous cell carcinoma
  JOURNAL   Oncotarget 7 (51), 85097-85108 (2016)
   PUBMED   27835882
  REMARK    GeneRIF: DDX60 is a novel and unfavorable biomarker for
            tumorigenesis and prognosis of oral squamous cell carcinoma in a
            subsite-specific manner
REFERENCE   8  (bases 1 to 6015)
  AUTHORS   Oshiumi,H., Miyashita,M., Okamoto,M., Morioka,Y., Okabe,M.,
            Matsumoto,M. and Seya,T.
  TITLE     DDX60 Is Involved in RIG-I-Dependent and Independent Antiviral
            Responses, and Its Function Is Attenuated by Virus-Induced EGFR
            Activation
  JOURNAL   Cell Rep 11 (8), 1193-1207 (2015)
   PUBMED   25981042
  REMARK    GeneRIF: Results define DDX60 as a sentinel for cytoplasmic
            antiviral response, which is counteracted by virus-mediated EGF
            receptor activation.
REFERENCE   9  (bases 1 to 6015)
  AUTHORS   Miyashita,M., Oshiumi,H., Matsumoto,M. and Seya,T.
  TITLE     DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting
            RIG-I-like receptor-mediated signaling
  JOURNAL   Mol Cell Biol 31 (18), 3802-3819 (2011)
   PUBMED   21791617
  REMARK    GeneRIF: DDX60 is a novel antiviral helicase promoting RIG-I-like
            receptor-mediated signaling.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK307404.1, AK001649.1,
            BC038115.2, BC074781.2 and BC020601.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_017631.5.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are
            implicated in a number of cellular procsses involving RNA binding
            and alteration of RNA secondary structure. This gene encodes a
            DEXD/H box RNA helicase that functions as an antiviral factor and
            promotes RIG-I-like receptor-mediated signaling. [provided by
            RefSeq, Apr 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038115.2, SRR1660807.174261.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393743.8/ ENSP00000377344.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-834               AK307404.1         56-889
            835-1479            AK001649.1         766-1410
            1480-2050           AK307404.1         1535-2105
            2051-3758           AK001649.1         1982-3689
            3759-4389           BC038115.2         3759-4389
            4390-5399           BC074781.2         224-1233
            5400-6015           BC020601.1         671-1286
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q32.3"
     gene            1..6015
                     /gene="DDX60"
                     /note="DExD/H-box helicase 60"
                     /db_xref="GeneID:55601"
                     /db_xref="HGNC:HGNC:25942"
                     /db_xref="MIM:613974"
     exon            1..131
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            132..241
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..5376
                     /gene="DDX60"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable ATP-dependent RNA helicase DDX60; DEAD box
                     protein 60; DEAD (Asp-Glu-Ala-Asp) box polypeptide 60;
                     DEAD-box helicase 60"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX60
                     isoform 1"
                     /protein_id="NP_060101.3"
                     /db_xref="CCDS:CCDS34097.1"
                     /db_xref="GeneID:55601"
                     /db_xref="HGNC:HGNC:25942"
                     /db_xref="MIM:613974"
                     /translation="MERNVLTTFSQEMSQLILNEMPKAEYSSLFNDFVESEFFLIDGD
                     SLLITCICEISFKPGQNLHFFYLVERYLVDLISKGGQFTIVFFKDAEYAYFNFPELLS
                     LRTALILHLQKNTTIDVRTTFSRCLSKEWGSFLEESYPYFLIVADEGLNDLQTQLFNF
                     LIIHSWARKVNVVLSSGQESDVLCLYAYLLPSMYRHQIFSWKNKQNIKDAYTTLLNQL
                     ERFKLSALAPLFGSLKWNNITEEAHKTVSLLTQVWPEGSDIRRVFCVTSCSLSLRMYH
                     RFLGNREPSSGQETEIQQVNSNCLTLQEMEDLCKLHCLTVVFLLHLPLSQRACARVIT
                     SHWAEDMKPLLQMKKWCEYFILRNIHTFEFWNLNLIHLSDLNDELLLKNIAFYYENEN
                     VKGLHLNLGDTIMKDYEYLWNTVSKLVRDFEVGQPFPLRTTKVCFLEKKPSPIKDSSN
                     EMVPNLGFIPTSSFVVDKFAGDILKDLPFLKSDDPIVTSLVKQKEFDELVHWHSHKPL
                     SDDYDRSRCQFDEKSRDPRVLRSVQKYHVFQRFYGNSLETVSSKIIVTQTIKSKKDFS
                     GPKSKKAHETKAEIIARENKKRLFAREEQKEEQKWNALSFSIEEQLKENLHSGIKSLE
                     DFLKSCKSSCVKLQVEMVGLTACLKAWKEHCRSEEGKTTKDLSIAVQVMKRIHSLMEK
                     YSELLQEDDRQLIARCLKYLGFDELASSLHPAQDAENDVKVKKRNKYSVGIGPARFQL
                     QYMGHYLIRDERKDPDPRVQDFIPDTWQRELLDVVDKNESAVIVAPTSSGKTYASYYC
                     MEKVLKESDDGVVVYVAPTKALVNQVAATVQNRFTKNLPSGEVLCGVFTREYRHDALN
                     CQVLITVPACFEILLLAPHRQNWVKKIRYVIFDEVHCLGGEIGAEIWEHLLVMIRCPF
                     LALSATISNPEHLTEWLQSVKWYWKQEDKIIENNTASKRHVGRQAGFPKDYLQVKQSY
                     KVRLVLYGERYNDLEKHVCSIKHGDIHFDHFHPCAALTTDHIERYGFPPDLTLSPRES
                     IQLYDAMFQIWKSWPRAQELCPENFIHFNNKLVIKKMDARKYEESLKAELTSWIKNGN
                     VEQARMVLQNLSPEADLSPENMITMFPLLVEKLRKMEKLPALFFLFKLGAVENAAESV
                     STFLKKKQETKRPPKADKEAHVMANKLRKVKKSIEKQKIIDEKSQKKTRNVDQSLIHE
                     AEHDNLVKCLEKNLEIPQDCTYADQKAVDTETLQKVFGRVKFERKGEELKALAERGIG
                     YHHSAMSFKEKQLVEILFRKGYLRVVTATGTLALGVNMPCKSVVFAQNSVYLDALNYR
                     QMSGRAGRRGQDLMGDVYFFDIPFPKIGKLIKSNVPELRGHFPLSITLVLRLMLLASK
                     GDDPEDAKAKVLSVLKHSLLSFKQPRVMDMLKLYFLFSLQFLVKEGYLDQEGNPMGFA
                     GLVSHLHYHEPSNLVFVSFLVNGLFHDLCQPTRKGSKHFSQDVMEKLVLVLAHLFGRR
                     YFPPKFQDAHFEFYQSKVFLDDLPEDFSDALDEYNMKIMEDFTTFLRIVSKLADMNQE
                     YQLPLSKIKFTGKECEDSQLVSHLMSCKEGRVAISPFVCLSGNFDDDLLRLETPNHVT
                     LGTIGVNRSQAPVLLSQKFDNRGRKMSLNAYALDFYKHGSLIGLVQDNRMNEGDAYYL
                     LKDFALTIKSISVSLRELCENEDDNVVLAFEQLSTTFWEKLNKV"
     exon            242..311
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            312..501
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            502..843
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            844..960
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1119
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1278
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1420
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1576
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1682
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1798
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1959
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2215
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2382
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2541
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2691
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2811
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2907
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3023
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3024..3166
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3276
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3381
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3382..3510
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3511..3635
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3794
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3795..3942
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            3943..4095
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4096..4200
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..4401
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4402..4506
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4507..4648
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4649..4770
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4771..4918
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            4919..5061
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            5062..5213
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            5214..5276
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     exon            5277..6015
                     /gene="DDX60"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5992..5997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX60"
                     /note="hexamer: AATAAA"
     polyA_site      6015
                     /gene="DDX60"
                     /note="major polyA site"
ORIGIN      
        1 gccgagtcct aggccaggtc tggggtaacc tggaacttcc acctgggctc tgcgctaggt
       61 ctctgtttca ctccctcccc gcggggcgcg cagctcgcgg gtctttggac accaccggtc
      121 ctgagtccgc ggactgccat tttcattaag aactgccact tagaggtacc aaaataaagg
      181 gtatttgcta cctttaatac ttgccagttc aggttggagg cacaggcagc agcaagaatg
      241 gaaagaaatg ttcttacaac attttcacag gaaatgtccc agttaatttt gaatgaaatg
      301 ccaaaagctg aatattccag tttattcaat gattttgttg aatctgaatt ttttttgatt
      361 gatggggatt cattacttat cacatgtatc tgtgagatat catttaagcc tgggcagaac
      421 ctccatttct tctatctggt tgaacgctat cttgtggatc ttattagcaa aggaggacaa
      481 ttcaccatag ttttcttcaa ggatgccgag tatgcgtatt tcaacttccc tgaacttctt
      541 tctttgagaa ctgctttaat tcttcatctt cagaagaata ccaccattga tgttcgaaca
      601 acattttcga gatgcttatc aaaagagtgg ggaagtttct tggaagagag ttacccatat
      661 ttcctgatag ttgcagacga aggcctgaac gatctacaaa cacagctttt caacttttta
      721 atcattcatt cttgggcaag gaaggtcaac gttgtacttt cctcagggca agaatctgat
      781 gttctttgcc tttatgcata ccttcttcca agcatgtaca gacaccagat tttttcctgg
      841 aagaataagc agaacattaa agatgcttat acaaccctgc ttaaccagtt ggaaagattt
      901 aagctttcag cattagcacc tctttttgga agtttaaaat ggaataatat tacggaagag
      961 gcacacaaga ctgtatctct gcttacacaa gtctggccag aaggatctga cattcggcgt
     1021 gtcttttgtg ttacttcatg ctcattatct ttgagaatgt accatcgctt tttaggaaac
     1081 agagagccct cctctggtca ggaaactgag atccaacagg tgaacagtaa ttgcttaacc
     1141 ctgcaggaga tggaagattt gtgtaaactg cattgtctca ctgtggtttt tctactccat
     1201 ctgcctcttt ctcaaagagc ttgtgctaga gtcatcactt cccattgggc tgaggacatg
     1261 aagcctttat tacaaatgaa aaagtggtgt gaatatttca tcttaagaaa tatacatact
     1321 tttgaatttt ggaatctgaa tttaattcac ctttctgact taaatgatga gcttttgttg
     1381 aagaatattg ctttttacta tgaaaatgaa aatgtaaaag gcctacattt gaatttggga
     1441 gataccatta tgaaagatta tgaatatctc tggaataccg tatcaaagtt ggtcagagac
     1501 tttgaggttg gacagccatt tcctctgaga acaacaaaag tttgttttct tgaaaagaaa
     1561 ccatcaccaa tcaaagacag ctccaatgaa atggtgccca atttgggttt tattccaacg
     1621 tcatcttttg tggttgataa atttgctgga gatattttga aagatttgcc ttttctaaag
     1681 agtgatgatc ctattgttac ttcactggtt aaacaaaagg aatttgatga acttgtgcac
     1741 tggcattctc ataaacccct gagtgatgat tatgacaggt ccaggtgtca gtttgatgaa
     1801 aaatctagag accctcgtgt tcttagatct gtgcaaaagt atcatgtttt ccaacggttt
     1861 tatgggaatt cattagaaac agtctcttcg aaaatcatcg tgactcaaac tattaagtca
     1921 aagaaggatt ttagtgggcc caagagcaaa aaggcacacg agaccaaggc tgaaataatt
     1981 gctagagaga ataagaaaag gttatttgcc agggaagaac aaaaggaaga gcaaaagtgg
     2041 aatgctttgt cattttctat tgaagagcaa ttgaaagaaa atttacactc tggaataaag
     2101 agcctggaag attttttgaa atcctgtaaa agtagctgtg tgaaacttca ggttgaaatg
     2161 gtggggttaa ctgcttgctt gaaagcctgg aaagaacatt gccgaagtga agaaggtaaa
     2221 accacgaaag atttaagtat agctgttcag gtgatgaaaa ggatccactc cttgatggaa
     2281 aaatactcag aacttttaca agaagatgat cggcaactca tagccagatg ccttaagtat
     2341 ttaggatttg atgagttggc aagttcttta catccagccc aggatgcaga aaatgatgta
     2401 aaagtgaaga aaaggaataa atattcagtt ggcattgggc cagctcggtt ccaactgcaa
     2461 tacatgggcc attatttgat acgagatgag agaaaagacc cagatcccag ggtccaggat
     2521 tttattcccg acacatggca gcgagagctc cttgatgttg tggataagaa tgagtcagca
     2581 gtgattgttg ccccaacgtc ctcaggcaaa acctatgcct cctactactg tatggagaaa
     2641 gtgctgaagg agagcgacga cggggtggtc gtgtacgttg cacccacaaa ggcccttgtt
     2701 aatcaagtgg cagcaactgt tcagaatcgt tttacgaaaa atctgccaag tggtgaagtt
     2761 ctctgtggtg ttttcaccag ggagtatcgt catgatgcct taaactgtca ggtacttatt
     2821 acagtgcctg cctgctttga aattctgctg cttgctcctc atcgccaaaa ctgggtgaaa
     2881 aagatcagat atgttatatt tgatgaggtt cattgtcttg gtggagaaat tggagcagaa
     2941 atctgggaac atctccttgt catgatccga tgtccctttt tggctctttc agctaccata
     3001 agtaatcctg aacatctcac cgagtggcta caatcggtaa aatggtactg gaaacaagaa
     3061 gacaaaataa ttgaaaataa taccgcttct aaaagacatg tgggtcgtca ggccggcttt
     3121 cccaaagact acttgcaagt aaaacaatcg tataaagtta gacttgtgct ctatggagag
     3181 aggtataatg atctagagaa gcatgtatgt tcaataaaac atggtgacat tcattttgat
     3241 cattttcacc catgtgctgc actaacaaca gatcatattg aaaggtatgg attccctcct
     3301 gatcttaccc tttcacctcg agaaagcatc cagctgtatg atgccatgtt tcaaatttgg
     3361 aaaagttggc ctcgggccca ggaactgtgc ccagaaaact tcattcattt taacaataaa
     3421 ttagtcatta aaaagatgga tgctaggaaa tatgaagaga gtctaaaggc agaattaaca
     3481 agttggatta aaaatggcaa cgtagagcag gccagaatgg tacttcagaa tcttagtcct
     3541 gaagcagatt tgagtccaga aaacatgatc accatgtttc cacttctagt tgaaaaacta
     3601 aggaaaatgg agaagttacc tgcactattt tttttattca agttaggagc tgtagaaaac
     3661 gcagctgaaa gtgtgagcac tttcctaaag aaaaagcagg agacaaaaag gcctcccaaa
     3721 gctgataaag aagcccatgt catggctaac aaacttcgaa aagttaaaaa atccatagag
     3781 aaacaaaaga tcatagatga aaagagccag aaaaaaacca gaaatgtgga tcaaagccta
     3841 atacatgaag ctgaacatga taatctagtg aagtgtctag agaagaacct ggaaatccca
     3901 caggactgca catatgctga tcaaaaagca gtggacactg agactttgca gaaggtattt
     3961 ggtcgagtaa aatttgaaag aaaaggtgaa gaattgaaag ccttggcaga aaggggtatt
     4021 ggatatcatc acagtgctat gagtttcaaa gaaaaacaat tagttgaaat cctctttaga
     4081 aaaggatatc ttagggtggt gacagctact ggaacacttg ctttaggtgt caacatgcct
     4141 tgtaaatctg tggtttttgc tcaaaactca gtctatctgg atgcgttgaa ttatagacag
     4201 atgtctggcc gtgctggaag aagaggtcaa gacctgatgg gagatgtata tttctttgat
     4261 attccattcc ccaaaatagg aaaactcata aaatccaatg ttcctgagct gagaggacac
     4321 ttccctctca gcataaccct ggtcctgcga ctcatgctgc tggcttccaa gggagatgac
     4381 ccagaggatg ccaaggcaaa ggtgctatca gtgctaaagc attcattgct gtccttcaag
     4441 caacccagag tcatggacat gttaaaactt tacttcctgt tttctttgca gttcctggtg
     4501 aaagagggct atttagatca agaaggtaat cctatggggt ttgctggact tgtatcacat
     4561 ttgcattatc atgaaccttc taatcttgtt tttgtcagtt ttcttgtaaa tggactcttc
     4621 catgatctct gtcagccaac caggaaaggc tcaaaacatt tttctcaaga cgttatggaa
     4681 aagctagtat tagtattggc acatctcttt ggaagaagat attttccacc aaagttccaa
     4741 gatgcacact tcgagtttta tcaatcaaag gtgttccttg atgatctccc tgaggatttt
     4801 agtgatgctt tagatgaata taacatgaaa attatggagg actttaccac tttcctacga
     4861 attgtttcca aactggctga tatgaatcag gaatatcaac tcccattgtc aaaaatcaaa
     4921 ttcacaggta aagaatgtga agactctcaa ctcgtatctc atttgatgag ctgcaaggaa
     4981 ggaagagtag caatttcacc atttgtttgt ctgtctggga actttgatga tgatttgctt
     5041 cgactagaaa ctccaaacca tgttactcta ggcacaatcg gtgtcaatcg ctctcaggct
     5101 ccagtgctgt tgtcacagaa atttgataac cgaggaagga aaatgtcgct taatgcctat
     5161 gcactggatt tctacaaaca tggttccttg ataggattag tccaggataa caggatgaat
     5221 gaaggagatg cttattattt gttgaaggat tttgcactca ccattaaatc tatcagtgtt
     5281 tccttgcgtg agctatgtga aaatgaagac gacaacgttg tcttagcctt tgaacaactg
     5341 agtacaactt tttgggaaaa gttaaacaaa gtctaaaaac aaagtctatg caaaccactt
     5401 aaaaataatt ccatagtagt ttttcaggtc acgtttttga ttcttatgct tcttgccaga
     5461 aatacattat gataaagtgg aaatacatta cgatgaagtg gaaagagcaa acactttgga
     5521 atcaaacaga gttgcaatca aacctgccat gttctgtcat gaatactcac aaattattta
     5581 gtatacctga atcttggttt ctttttataa ctgagtaata atggttacat ctcaggtagt
     5641 ttgaggattg actaaaaaaa tgcgagaatg ttgtatgtga ctgaataaca atttttactc
     5701 tgcgaagcca aagtaaatat aatattatca gtaactttat ccccagtgtc agtatttata
     5761 aaatgtttat taaggctaga aaaaatgaat acaatatcct gaaggtgaaa tatattctct
     5821 tcaattagca taaatatgat ttacataagt tagctataca gctattgaga tagtactttc
     5881 tagtaaactt aaactacttt ttaaacatac attttgtgat gatttaacaa aaatatagag
     5941 aatgatttgc tttattgtaa ttgtatataa gtgactggaa aagcacaaag aaataaagtg
     6001 ggttcgatct gttta
//
