LOCUS       NM_024045               2501 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DExD-box helicase 50 (DDX50), mRNA.
ACCESSION   NM_024045
VERSION     NM_024045.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Pallett,M.A., Lu,Y. and Smith,G.L.
  TITLE     DDX50 Is a Viral Restriction Factor That Enhances IRF3 Activation
  JOURNAL   Viruses 14 (2), 316 (2022)
   PUBMED   35215908
  REMARK    GeneRIF: DDX50 Is a Viral Restriction Factor That Enhances IRF3
            Activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Han,P., Ye,W., Lv,X., Ma,H., Weng,D., Dong,Y., Cheng,L., Chen,H.,
            Zhang,L., Xu,Z., Lei,Y. and Zhang,F.
  TITLE     DDX50 inhibits the replication of dengue virus 2 by upregulating
            IFN-beta production
  JOURNAL   Arch Virol 162 (6), 1487-1494 (2017)
   PUBMED   28181036
  REMARK    GeneRIF: Results provide evidence that DDX50 negatively regulates
            DENV-2 replication during the early stages of infection by inducing
            IFN-beta production.
REFERENCE   3  (bases 1 to 2501)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (bases 1 to 2501)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (bases 1 to 2501)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 2501)
  AUTHORS   Ohnishi,S., Paakkonen,K., Koshiba,S., Tochio,N., Sato,M.,
            Kobayashi,N., Harada,T., Watanabe,S., Muto,Y., Guntert,P.,
            Tanaka,A., Kigawa,T. and Yokoyama,S.
  TITLE     Solution structure of the GUCT domain from human RNA helicase II/Gu
            beta reveals the RRM fold, but implausible RNA interactions
  JOURNAL   Proteins 74 (1), 133-144 (2009)
   PUBMED   18615715
  REMARK    GeneRIF: Solution structure of the GUCT domain from human RNA
            helicase II/Gu beta reveals the RRM fold, but implausible RNA
            interactions.
REFERENCE   7  (bases 1 to 2501)
  AUTHORS   Andersen,J.S., Lam,Y.W., Leung,A.K., Ong,S.E., Lyon,C.E.,
            Lamond,A.I. and Mann,M.
  TITLE     Nucleolar proteome dynamics
  JOURNAL   Nature 433 (7021), 77-83 (2005)
   PUBMED   15635413
REFERENCE   8  (bases 1 to 2501)
  AUTHORS   Scherl,A., Coute,Y., Deon,C., Calle,A., Kindbeiter,K.,
            Sanchez,J.C., Greco,A., Hochstrasser,D. and Diaz,J.J.
  TITLE     Functional proteomic analysis of human nucleolus
  JOURNAL   Mol Biol Cell 13 (11), 4100-4109 (2002)
   PUBMED   12429849
REFERENCE   9  (bases 1 to 2501)
  AUTHORS   Valdez,B.C., Yang,H., Hong,E. and Sequitin,A.M.
  TITLE     Genomic structure of newly identified paralogue of RNA helicase
            II/Gu: detection of pseudogenes and multiple alternatively spliced
            mRNAs
  JOURNAL   Gene 284 (1-2), 53-61 (2002)
   PUBMED   11891046
REFERENCE   10 (bases 1 to 2501)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359844.15.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024045.1.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this DEAD box
            protein family are believed to be involved in embryogenesis,
            spermatogenesis, and cellular growth and division. This gene
            encodes a DEAD box enzyme that may be involved in ribosomal RNA
            synthesis or processing. This gene and DDX21, also called
            RH-II/GuA, have similar genomic structures and are in tandem
            orientation on chromosome 10, suggesting that the two genes arose
            by gene duplication in evolution. This gene has pseudogenes on
            chromosomes 2, 3 and 4. Alternative splicing of this gene generates
            multiple transcript variants, but the full length nature of all the
            other variants but one has not been defined. [provided by RefSeq,
            Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000272.1, SRR1803611.100878.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373585.8/ ENSP00000362687.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AL359844.15        42877-43033
            158-454             AL359844.15        48273-48569
            455-530             AL359844.15        51869-51944
            531-709             AL359844.15        52630-52808
            710-827             AL359844.15        54724-54841
            828-1013            AL359844.15        54953-55138
            1014-1159           AL359844.15        55621-55766
            1160-1309           AL359844.15        61394-61543
            1310-1471           AL359844.15        75761-75922
            1472-1591           AL359844.15        76361-76480
            1592-1665           AL359844.15        77568-77641
            1666-1825           AL359844.15        78498-78657
            1826-1960           AL359844.15        82622-82756
            1961-2005           AL359844.15        84775-84819
            2006-2501           AL359844.15        87914-88409
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..2501
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /note="DExD-box helicase 50"
                     /db_xref="GeneID:79009"
                     /db_xref="HGNC:HGNC:17906"
                     /db_xref="MIM:610373"
     exon            1..157
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..2284
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /EC_number="3.6.4.13"
                     /note="RNA helicase II/Gu beta; nucleolar protein GU2;
                     DEAD box protein 50; malignant cell derived RNA helicase;
                     gu-beta; DEAD (Asp-Glu-Ala-Asp) box polypeptide 50;
                     DEAD-box helicase 50"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DDX50"
                     /protein_id="NP_076950.1"
                     /db_xref="CCDS:CCDS7283.1"
                     /db_xref="GeneID:79009"
                     /db_xref="HGNC:HGNC:17906"
                     /db_xref="MIM:610373"
                     /translation="MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEK
                     SETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDID
                     EYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYL
                     FPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLA
                     PTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQS
                     GRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVY
                     KVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRA
                     IIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAAR
                     GLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAG
                     ITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAAL
                     AHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMC
                     LLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGW
                     SSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD"
     exon            158..454
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            455..530
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            531..709
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            710..827
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1013
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1159
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1309
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1471
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1591
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1665
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1825
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1960
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2005
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2501
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2469..2474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /note="hexamer: AATAAA"
     polyA_site      2501
                     /gene="DDX50"
                     /gene_synonym="GU2; GUB; mcdrh; RH-II/GuB"
                     /note="major polyA site"
ORIGIN      
        1 cctttcacgc tgtcgctgcc cgtaggtggt tgtggccact gtgcccggag ggaggcggcg
       61 gtggccagta atgcctggga aactcctctg gggggacatt atggagctgg aagcaccctt
      121 ggaggagtcc gagagccaga agaaggagag gcaaaagagt gacagaagga agtcaaggca
      181 ccattatgac tcggatgaga aatcagaaac aagagaaaat ggtgttacag atgacctgga
      241 tgctcccaag gccaaaaaat ctaaaatgaa agagaagcta aatggagaca ctgaagaagg
      301 atttaataga ctttcagatg aattctccaa atctcataag tcaagaagaa aagatctacc
      361 aaatggagat atagatgaat atgaaaaaaa atcaaagcga gtatcatctt tagatacttc
      421 tactcataaa tcaagtgata ataaactaga ggagacctta acacgtgaac agaaagaagg
      481 agccttctcc aattttccta tttctgaaga gactataaag cttctgaaag gtcgaggggt
      541 aacatatctc tttcctattc aagttaagac ctttggtcct gtatatgaag gaaaagattt
      601 aatagctcaa gcacggacag gaacaggaaa gacattctct tttgccatcc ccttaattga
      661 aagactccaa agaaatcaag aaacaattaa aaaaagccgc tcaccaaagg tacttgtttt
      721 ggctccaaca agggaactgg caaaccaagt agccaaagac ttcaaagata taactaggaa
      781 actcagcgtg gcgtgttttt atggtggaac atcatatcaa agccaaatta atcatattcg
      841 aaatggtatt gacatcttgg ttggaacacc tggtcgtatc aaagaccatc tgcagagtgg
      901 ccgattggat ctttctaaac tgcgacatgt tgtgcttgat gaagtggatc agatgttaga
      961 tttaggtttc gctgaacaag ttgaagatat tattcatgaa tcctacaaaa ctgattctga
     1021 agacaatcct cagactttac ttttttctgc aacttgccca cagtgggtat acaaagttgc
     1081 aaaaaaatac atgaaatcca gatatgaaca ggttgacctt gttggaaaaa tgactcaaaa
     1141 ggctgcaact actgtggaac atttggccat ccagtgtcat tggtctcaga ggccagcagt
     1201 tattggagat gtccttcaag tctacagtgg gtctgaaggg agggctatta ttttctgtga
     1261 gaccaagaag aatgtaactg aaatggccat gaatccacac ataaaacaga atgcccagtg
     1321 tttacatggg gacattgcac agtcacaaag agaaattaca ctaaaaggct tcagagaagg
     1381 tagttttaaa gttttggtgg caaccaatgt ggctgcccgt ggtttggaca ttcctgaagt
     1441 tgacctggtg attcaaagtt ctcctcctca ggatgttgag tcctatatcc atcgctctgg
     1501 acgcacaggt agagctggac ggacagggat ttgtatatgt ttttatcaac caagagaaag
     1561 aggtcaacta agatatgtgg aacaaaaagc aggaattact tttaaacgtg taggtgttcc
     1621 ttctacaatg gatttagtta aatctaaaag catggatgcc atcaggtctc tggcttccgt
     1681 ttcttacgct gctgttgatt ttttccgacc atcagctcag agactgatag aagagaaagg
     1741 tgcagtggat gcattggctg cagctttagc ccacatttct ggtgcatcaa gctttgaacc
     1801 acgatctttg atcacctctg ataaggggtt tgtgaccatg actctggaaa gcctagagga
     1861 aatacaggat gtcagctgtg cttggaaaga acttaacaga aagctgagta gtaatgcagt
     1921 gtctcagatt accagaatgt gcctcctgaa aggaaatatg ggtgtttgct ttgatgttcc
     1981 tacaactgag tcagaaaggt tacaggcaga gtggcatgat tccgactgga tactctcagt
     2041 gccagccaaa ttacctgaaa ttgaagaata ttatgatgga aacacatctt ctaattccag
     2101 acagaggagt ggctggtcaa gtggtcgatc aggccggtca ggccggtcag gtggtcgatc
     2161 tggcggccgg tcaggtagac agagtcgaca aggaagtcgc tcaggaagtc gacaagatgg
     2221 tagaagacga agtgggaata gaaatcgatc aagaagtggg ggccacaaac ggagttttga
     2281 ctgagtattt gatagttaat ctaccagtgt gagcttgcct atttctgcct aatcatgtac
     2341 attatccacc aaaaattagg tcatcatagt tgaggtatgt gtctgctatt tgcaaagaag
     2401 ttggtcgtat ttttttaaaa agtatttcac aagaatggaa caaatctact tatccagtta
     2461 tacctttgaa taaaaaaacc tccttttatt gtctcttttc a
//
