LOCUS       NM_004396               3684 bp    mRNA    linear   PRI 05-MAR-2025
DEFINITION  Homo sapiens DEAD-box helicase 5 (DDX5), transcript variant 2,
            mRNA.
ACCESSION   NM_004396
VERSION     NM_004396.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Zhu,X., Li,Y., Shao,Z., Lu,X. and Chen,Y.
  TITLE     NAP1L1 Promotes Endometrial Cancer Progression via EP300-Mediated
            DDX5 Promoter Acetylation
  JOURNAL   Mol Cancer Res 22 (11), 1011-1021 (2024)
   PUBMED   38953887
  REMARK    GeneRIF: NAP1L1 Promotes Endometrial Cancer Progression via
            EP300-Mediated DDX5 Promoter Acetylation.
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Shi,Y., Wang,J., Yuan,Q., Chen,Y., Zhao,M., Li,X., Wang,Z.,
            Zhou,H., Zhu,F., Wei,B., Jiang,Y., Zhao,J., Qiao,Y., Dong,Z. and
            Liu,K.
  TITLE     DDX5 promotes esophageal squamous cell carcinoma growth through
            sustaining VAV3 mRNA stability
  JOURNAL   Oncogene 43 (44), 3240-3254 (2024)
   PUBMED   39289531
  REMARK    GeneRIF: DDX5 promotes esophageal squamous cell carcinoma growth
            through sustaining VAV3 mRNA stability.
REFERENCE   3  (bases 1 to 3684)
  AUTHORS   Chapus,F., Giraud,G., Huchon,P., Roda,M., Grand,X., Charre,C.,
            Goldsmith,C., Roca Suarez,A.A., Martinez,M.G., Fresquet,J.,
            Diederichs,A., Locatelli,M., Polveche,H., Scholtes,C., Chemin,I.,
            Hernandez Vargas,H., Rivoire,M., Bourgeois,C.F., Zoulim,F. and
            Testoni,B.
  TITLE     Helicases DDX5 and DDX17 promote heterogeneity in HBV transcription
            termination in infected human hepatocytes
  JOURNAL   J Hepatol 81 (4), 609-620 (2024)
   PUBMED   38782119
  REMARK    GeneRIF: Helicases DDX5 and DDX17 promote heterogeneity in HBV
            transcription termination in infected human hepatocytes.
REFERENCE   4  (bases 1 to 3684)
  AUTHORS   Li,S. and Wang,Y.
  TITLE     Regulatory mechanism of DDX5 in ox-LDL-induced endothelial cell
            injury through the miR-640/SOX6 axis
  JOURNAL   Clin Hemorheol Microcirc 88 (2), 157-170 (2024)
   PUBMED   39093065
  REMARK    GeneRIF: Regulatory mechanism of DDX5 in ox-LDL-induced endothelial
            cell injury through the miR-640/SOX6 axis.
REFERENCE   5  (bases 1 to 3684)
  AUTHORS   Wang,Z., Luo,Z., Zhou,L., Li,X., Jiang,T. and Fu,E.
  TITLE     DDX5 promotes proliferation and tumorigenesis of non-small-cell
            lung cancer cells by activating beta-catenin signaling pathway
  JOURNAL   Cancer Sci 106 (10), 1303-1312 (2015)
   PUBMED   26212035
  REMARK    GeneRIF: DDX5 played an important role in the proliferation and
            tumorigenesis of non-small-cell lung cancer cells by activating the
            beta-catenin signaling pathway.
REFERENCE   6  (bases 1 to 3684)
  AUTHORS   Fuller-Pace,F.V. and Moore,H.C.
  TITLE     RNA helicases p68 and p72: multifunctional proteins with important
            implications for cancer development
  JOURNAL   Future Oncol 7 (2), 239-251 (2011)
   PUBMED   21345143
  REMARK    Review article
REFERENCE   7  (bases 1 to 3684)
  AUTHORS   Bates,G.J., Nicol,S.M., Wilson,B.J., Jacobs,A.M., Bourdon,J.C.,
            Wardrop,J., Gregory,D.J., Lane,D.P., Perkins,N.D. and
            Fuller-Pace,F.V.
  TITLE     The DEAD box protein p68: a novel transcriptional coactivator of
            the p53 tumour suppressor
  JOURNAL   EMBO J 24 (3), 543-553 (2005)
   PUBMED   15660129
  REMARK    GeneRIF: mechanism by which p68 may act as a tumour cosuppressor in
            governing p53 transcriptional activity
REFERENCE   8  (bases 1 to 3684)
  AUTHORS   Iggo,R.D., Jamieson,D.J., MacNeill,S.A., Southgate,J., McPheat,J.
            and Lane,D.P.
  TITLE     p68 RNA helicase: identification of a nucleolar form and cloning of
            related genes containing a conserved intron in yeasts
  JOURNAL   Mol Cell Biol 11 (3), 1326-1333 (1991)
   PUBMED   1996094
REFERENCE   9  (bases 1 to 3684)
  AUTHORS   Hloch,P., Schiedner,G. and Stahl,H.
  TITLE     Complete cDNA sequence of the human p68 protein
  JOURNAL   Nucleic Acids Res 18 (10), 3045 (1990)
   PUBMED   2349099
REFERENCE   10 (bases 1 to 3684)
  AUTHORS   Ford,M.J., Anton,I.A. and Lane,D.P.
  TITLE     Nuclear protein with sequence homology to translation initiation
            factor eIF-4A
  JOURNAL   Nature 332 (6166), 736-738 (1988)
   PUBMED   2451786
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB133401.1, BC016027.1,
            BU160611.1, CN280686.1, BM695261.1 and N67237.1.
            
            On May 17, 2019 this sequence version replaced NM_004396.4.
            
            Summary: This gene encodes a member of the DEAD box family of RNA
            helicases that are involved in a variety of cellular processes as a
            result of its role as an adaptor molecule, promoting interactions
            with a large number of other factors. This protein is involved in
            pathways that include the alteration of RNA structures, plays a
            role as a coregulator of transcription, a regulator of splicing,
            and in the processing of small noncoding RNAs. Members of this
            family contain nine conserved motifs, including the conserved
            Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and
            hydrolysis as well as RNA binding and unwinding activities.
            Dysregulation of this gene may play a role in cancer development.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 through 3 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1106477.1,
                                           SRR3476690.837946.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146, SAMN03267750
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000225792.10/ ENSP00000225792.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DB133401.1         233-261
            30-2318             BC016027.1         1-2289
            2319-2525           BU160611.1         409-615
            2526-3119           CN280686.1         68-661
            3120-3420           BM695261.1         62-362
            3421-3684           N67237.1           23-286              c
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.3"
     gene            1..3684
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /note="DEAD-box helicase 5"
                     /db_xref="GeneID:1655"
                     /db_xref="HGNC:HGNC:2746"
                     /db_xref="MIM:180630"
     exon            1..214
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..2015
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /EC_number="3.6.4.13"
                     /note="isoform a is encoded by transcript variant 2;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA
                     helicase, 68kD); DEAD box-5; DEAD (Asp-Glu-Ala-Asp) box
                     helicase 5; ATP-dependent RNA helicase DDX5; RNA helicase
                     p68; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box
                     polypeptide 5"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX5 isoform
                     a"
                     /protein_id="NP_004387.1"
                     /db_xref="CCDS:CCDS11659.1"
                     /db_xref="GeneID:1655"
                     /db_xref="HGNC:HGNC:2746"
                     /db_xref="MIM:180630"
                     /translation="MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKK
                     KWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFP
                     ANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQP
                     FLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGV
                     EICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTL
                     MWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLME
                     EIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKA
                     PILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNI
                     KQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTF
                     RDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGT
                     YQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ"
     exon            215..380
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            381..477
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            478..611
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            612..677
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            678..819
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            820..980
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1153
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1264
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1326
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1386
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1611
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..3684
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2295..2300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /note="hexamer: AATAAA"
     polyA_site      2318
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /note="major polyA site"
     regulatory      3662..3667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
                     /note="hexamer: AATAAA"
     polyA_site      3684
                     /gene="DDX5"
                     /gene_synonym="G17P1; HLR1; HUMP68; p68"
ORIGIN      
        1 acctcattca tttctaccgg tctctagtag tgcagcttcg gctggtgtca tcggtgtcct
       61 tcctccgctg ccgcccccgc aaggcttcgc cgtcatcgag gccatttcca gcgacttgtc
      121 gcacgctttt ctatatactt cgttccccgc caaccgcaac cattgacgcc atgtcgggtt
      181 attcgagtga ccgagaccgc ggccgggacc gagggtttgg tgcacctcga tttggaggaa
      241 gtagggcagg gcccttatct ggaaagaagt ttggaaaccc tggggagaaa ttagttaaaa
      301 agaagtggaa tcttgatgag ctgcctaaat ttgagaagaa tttttatcaa gagcaccctg
      361 atttggctag gcgcacagca caagaggtgg aaacatacag aagaagcaag gaaattacag
      421 ttagaggtca caactgcccg aagccagttc taaattttta tgaagccaat ttccctgcaa
      481 atgtcatgga tgttattgca agacagaatt tcactgaacc cactgctatt caagctcagg
      541 gatggccagt tgctctaagt ggattggata tggttggagt ggcacagact ggatctggga
      601 aaacattgtc ttatttgctt cctgccattg tccacatcaa tcatcagcca ttcctagaga
      661 gaggcgatgg gcctatttgt ttggtgctgg caccaactcg ggaactggcc caacaggtgc
      721 agcaagtagc tgctgaatat tgtagagcat gtcgcttgaa gtctacttgt atctacggtg
      781 gtgctcctaa gggaccacaa atacgtgatt tggagagagg tgtggaaatc tgtattgcaa
      841 cacctggaag actgattgac tttttagagt gtggaaaaac caatctgaga agaacaacct
      901 accttgtcct tgatgaagca gatagaatgc ttgatatggg ctttgaaccc caaataagga
      961 agattgtgga tcaaataaga cctgataggc aaactctaat gtggagtgcg acttggccaa
     1021 aagaagtaag acagcttgct gaagatttcc tgaaagacta tattcatata aacattggtg
     1081 cacttgaact gagtgcaaac cacaacattc ttcagattgt ggatgtgtgt catgacgtag
     1141 aaaaggatga aaaacttatt cgtctaatgg aagagatcat gagtgagaag gagaataaaa
     1201 ccattgtttt tgtggaaacc aaaagaagat gtgatgagct taccagaaaa atgaggagag
     1261 atgggtggcc tgccatgggt atccatggtg acaagagtca acaagagcgt gactgggttc
     1321 taaatgaatt caaacatgga aaagctccta ttctgattgc tacagatgtg gcctccagag
     1381 ggctagatgt ggaagatgtg aaatttgtca tcaattatga ctaccctaac tcctcagagg
     1441 attatattca tcgaattgga agaactgctc gcagtaccaa aacaggcaca gcatacactt
     1501 tctttacacc taataacata aagcaagtga gcgaccttat ctctgtgctt cgtgaagcta
     1561 atcaagcaat taatcccaag ttgcttcagt tggtcgaaga cagaggttca ggtcgttcca
     1621 ggggtagagg aggcatgaag gatgaccgtc gggacagata ctctgcgggc aaaaggggtg
     1681 gatttaatac ctttagagac agggaaaatt atgacagagg ttactctagc ctgcttaaaa
     1741 gagattttgg ggcaaaaact cagaatggtg tttacagtgc tgcaaattac accaatggga
     1801 gctttggaag taattttgtg tctgctggta tacagaccag ttttaggact ggtaatccaa
     1861 cagggactta ccagaatggt tatgatagca ctcagcaata cggaagtaat gttccaaata
     1921 tgcacaatgg tatgaaccaa caggcatatg catatcctgc tactgcagct gcacctatga
     1981 ttggttatcc aatgccaaca ggatattccc aataagactt tagaagtata tgtaaatgtc
     2041 tgtttttcat aattgctctt tatattgtgt gttatctgac aagatagtta tttaagaaac
     2101 atgggaattg cagaaatgac tgcagtgcag cagtaattat ggtgcacttt ttcgctattt
     2161 aagttggata tttctctaca ttcctgaaac aatttttagg ttttttttgt actagaaaat
     2221 gcaggcagtg ttttcacaaa agtaaatgta cagtgatttg aaatacaata aatgaaggca
     2281 atgcatggcc ttccaataaa aaatatttga agactgaatt aagtggaaat tgtactttat
     2341 tttatataat gtcatgtaaa actttgctta agatggtctg gttttttttt tgtttttgtt
     2401 tggttttttt tttccatgaa aacaaatgac tgttcctttt tatttaattt gggaggcagg
     2461 gggaatcaga aggcccttct ttataatgag ctattcatat tgcaggagtc agaatgaatt
     2521 gatacaggtg aatttttagt tacaggctaa attgcataaa agctttgtca gcttccagca
     2581 tcaggggagt catttaatag cctttttcct tatttgctag tatggttaaa tgagaaaata
     2641 gtaaaataga tacaaagtca tctatatagt gtgagaacgt gggtgacttt ttcaaagttt
     2701 ataatttaaa aagctccaaa taactggctt tttcaagaga cttatactca tgctcttggc
     2761 tatactgtga attactgaaa tgttgaacaa acctgtgaaa gacatacatt agccctttaa
     2821 gatggccagg agctaagctt gagtctcctt tactgaattt cgttcttagt gcaggttact
     2881 tgtagattct agtcttcaca ggctccctgg ggctcttaac tagtcacact gggagtcatg
     2941 aatgtctttc caataattca gggaattcta gagatcctca aactgtaagg tctattcata
     3001 ctcaacacaa ggaaaaaacc tcattaaaat taatgactaa tcaggaggca acgtaaccaa
     3061 aagcacagtg aatgaaagtt ttcatggtag gttcaacatg ggtttattgc tagaaagatc
     3121 caggggatag ctttaggttt aacttcggct caccaacgta actttctaat catttatttc
     3181 agtaatagct agaagtgggt ctgaatgttt tcccagagtc tgatacgtgt ttttttttgc
     3241 cagaagagag gtcttcagga gacttcattt aaattctgat tattaaactg aggctttaat
     3301 tgatgttaat gccttatgtc aaatgtaaag ttagaatttg ctagggctgg gatagggagt
     3361 gatatttcta ggacttagac attgaaaact aattcagcct gtagtaacct ggatggtttt
     3421 caatggcatg gttagtcaaa ttcatggttt taaacttaga agcagctttc gggggagagg
     3481 gtaggttgga gcatttatta catattttac tgtttaatgt cttaaccgtg ggccttttaa
     3541 tttgtaaaca ctgaaatgat tgttgggctg tggaaaacat ttacctattt accttggaag
     3601 ttttaaaaga cagtccactt tttagcatgt gtgttgtgtc cagcctgtgg tcgtcttaac
     3661 taataaatgt gatttttctc ccca
//
