LOCUS       NM_024415               2847 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1,
            mRNA.
ACCESSION   NM_024415 NM_001136034
VERSION     NM_024415.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Noyes,C., Kitajima,S., Li,F., Suita,Y., Miriyala,S., Isaac,S.,
            Ahsan,N., Knelson,E., Vajdi,A., Tani,T., Thai,T.C., Xu,D.,
            Murai,J., Tapinos,N., Takahashi,C., Barbie,D.A. and Yajima,M.
  TITLE     The germline factor DDX4 contributes to the chemoresistance of
            small cell lung cancer cells
  JOURNAL   Commun Biol 6 (1), 65 (2023)
   PUBMED   36653474
  REMARK    GeneRIF: The germline factor DDX4 contributes to the
            chemoresistance of small cell lung cancer cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Kim,J.A., Shen,S., Matson,D.R., Lovrien,L.N., Smith-Simmer,K.J.,
            Keles,S., Churpek,J.E. and Bresnick,E.H.
  TITLE     Discriminating activities of DEAD-Box Helicase 41 from myeloid
            malignancy-associated germline variants by genetic rescue
  JOURNAL   Leukemia 37 (1), 235-239 (2023)
   PUBMED   36347925
  REMARK    GeneRIF: Discriminating activities of DEAD-Box Helicase 41 from
            myeloid malignancy-associated germline variants by genetic rescue.
REFERENCE   3  (bases 1 to 2847)
  AUTHORS   Amirian,M., Azizi,H., Hashemi Karoii,D. and Skutella,T.
  TITLE     VASA protein and gene expression analysis of human non-obstructive
            azoospermia and normal by immunohistochemistry,
            immunocytochemistry, and bioinformatics analysis
  JOURNAL   Sci Rep 12 (1), 17259 (2022)
   PUBMED   36241908
  REMARK    GeneRIF: VASA protein and gene expression analysis of human
            non-obstructive azoospermia and normal by immunohistochemistry,
            immunocytochemistry, and bioinformatics analysis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2847)
  AUTHORS   D'Oronzo,S., Silvestris,E., Lovero,D., Cafforio,P., Duda,L.,
            Cormio,G., Paradiso,A., Palmirotta,R. and Silvestris,F.
  TITLE     DEAD-Box Helicase 4 (Ddx4)+ Stem Cells Sustain Tumor Progression in
            Non-Serous Ovarian Cancers
  JOURNAL   Int J Mol Sci 21 (17), 6096 (2020)
   PUBMED   32847044
  REMARK    GeneRIF: DEAD-Box Helicase 4 (Ddx4)(+) Stem Cells Sustain Tumor
            Progression in Non-Serous Ovarian Cancers.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2847)
  AUTHORS   Clarkson,Y.L., Weatherall,E., Waterfall,M., McLaughlin,M., Lu,H.,
            Skehel,P.A., Anderson,R.A. and Telfer,E.E.
  TITLE     Extracellular Localisation of the C-Terminus of DDX4 Confirmed by
            Immunocytochemistry and Fluorescence-Activated Cell Sorting
  JOURNAL   Cells 8 (6), 578 (2019)
   PUBMED   31212843
  REMARK    GeneRIF: This study clearly demonstrates that the C-terminus of
            DDX4 can be expressed on the cell surface despite its lack of a
            conventional membrane-targeting or secretory sequence.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2847)
  AUTHORS   Albamonte,M.S., Willis,M.A., Albamonte,M.I., Jensen,F.,
            Espinosa,M.B. and Vitullo,A.D.
  TITLE     The developing human ovary: immunohistochemical analysis of
            germ-cell-specific VASA protein, BCL-2/BAX expression balance and
            apoptosis
  JOURNAL   Hum Reprod 23 (8), 1895-1901 (2008)
   PUBMED   18534994
  REMARK    GeneRIF: VASA showed a differential pattern of expression
            throughout the differentiation and proliferative phase and prophase
            I to finally associate with Balbiani's body in primordial and
            primary follicles.
REFERENCE   7  (bases 1 to 2847)
  AUTHORS   Guo,X., Gui,Y.T., Tang,A.F., Lu,L.H., Gao,X. and Cai,Z.M.
  TITLE     Differential expression of VASA gene in ejaculated spermatozoa from
            normozoospermic men and patients with oligozoospermia
  JOURNAL   Asian J Androl 9 (3), 339-344 (2007)
   PUBMED   17486274
  REMARK    GeneRIF: The expression of VASA mRNA and protein was significantly
            decreased in the sperm of oligozoospermic men.
REFERENCE   8  (bases 1 to 2847)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N.,
            Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y.
            and Nakano,T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
REFERENCE   9  (bases 1 to 2847)
  AUTHORS   Zeeman,A.M., Stoop,H., Boter,M., Gillis,A.J., Castrillon,D.H.,
            Oosterhuis,J.W. and Looijenga,L.H.
  TITLE     VASA is a specific marker for both normal and malignant human germ
            cells
  JOURNAL   Lab Invest 82 (2), 159-166 (2002)
   PUBMED   11850529
  REMARK    GeneRIF: marker for normal and malignant germ cells
REFERENCE   10 (bases 1 to 2847)
  AUTHORS   Castrillon,D.H., Quade,B.J., Wang,T.Y., Quigley,C. and Crum,C.P.
  TITLE     The human VASA gene is specifically expressed in the germ cell
            lineage
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9585-9590 (2000)
   PUBMED   10920202
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC088362.1, DB508256.1 and AC016632.7.
            
            On May 17, 2019 this sequence version replaced NM_024415.2.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this family
            are believed to be involved in embryogenesis, spermatogenesis, and
            cellular growth and division. This gene encodes a DEAD box protein,
            which is a homolog of VASA proteins in Drosophila and several other
            species. The gene is specifically expressed in the germ cell
            lineage in both sexes and functions in germ cell development.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292417.1, BC088362.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000505374.6/ ENSP00000424838.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2467              BC088362.1         4-2470
            2468-2836           DB508256.1         49-417
            2837-2847           AC016632.7         32632-32642         c
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q11.2"
     gene            1..2847
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /note="DEAD-box helicase 4"
                     /db_xref="GeneID:54514"
                     /db_xref="HGNC:HGNC:18700"
                     /db_xref="MIM:605281"
     exon            1..41
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            42..124
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2230
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; probable
                     ATP-dependent RNA helicase DDX4; vasa homolog; DEAD box
                     protein 4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX4 isoform
                     1"
                     /protein_id="NP_077726.1"
                     /db_xref="CCDS:CCDS3969.1"
                     /db_xref="GeneID:54514"
                     /db_xref="HGNC:HGNC:18700"
                     /db_xref="MIM:605281"
                     /translation="MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEM
                     DDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFE
                     DGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGF
                     GLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEV
                     ITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILV
                     EVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQT
                     GSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGT
                     CVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADR
                     MLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQV
                     GGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKIS
                     TTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEY
                     VHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPG
                     FSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD"
     exon            125..182
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            183..260
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            261..338
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            339..389
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            390..449
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            450..551
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            552..632
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            633..680
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            681..728
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            729..776
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            777..919
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1072
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1227
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1357
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1524
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1670
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1770
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..2041
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2152
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2847
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2448..2453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /note="hexamer: ATTAAA"
     polyA_site      2467
                     /gene="DDX4"
                     /gene_synonym="VASA"
                     /note="major polyA site"
ORIGIN      
        1 gtgggcgcct gcgagggctt gggagagcaa gccgcggaga gaacttgaag ccaccatggg
       61 agatgaagat tgggaagcag aaatcaaccc tcatatgtct tcctatgttc ccatatttga
      121 gaaggatagg tattctggag aaaatggaga caattttaac aggactccag cttcatcatc
      181 agaaatggat gatggacctt ctcgaagaga tcatttcatg aaaagtggat ttgcctctgg
      241 gcggaatttt ggaaacagag atgctggtga gtgtaataag cgagataata catccacaat
      301 gggtggtttt ggagttggaa agagttttgg aaacagaggt ttttcaaaca gcaggtttga
      361 agatggtgat agctctggtt tctggagaga gtctagtaat gactgcgaag ataatccaac
      421 acggaacaga gggttttcca agagaggcgg ctatcgagat ggaaataatt cagaagcttc
      481 agggccatac agaagaggtg gaagaggtag tttccgaggt tgccgtggag gatttggtct
      541 aggaagtcca aataatgact tagacccaga cgaatgtatg cagcgcactg gtggcctttt
      601 tggttctaga agaccagtat taagtggcac aggtaatggt gatacttctc aaagcagaag
      661 tggcagtgga agtgaacgag gtggttacaa aggtttaaat gaagaagtaa taacaggctc
      721 tggaaagaat tcttggaagt cagaagcaga aggaggagaa agtagtgata ctcaaggacc
      781 aaaagtgacc tacatacccc ctcctccacc tgaggatgag gactccatct ttgcacatta
      841 tcagacaggc ataaacttcg acaaatacga cactattctt gtggaagtgt ctggacatga
      901 tgcaccacca gcaattctga cttttgaaga agctaatctc tgtcagacac tgaataacaa
      961 cattgctaaa gctggttata ctaagcttac tcctgtgcaa aaatacagta ttcctatcat
     1021 acttgcagga cgagatttga tggcttgcgc tcaaacaggg tctgggaaga ctgcggcttt
     1081 tctcctacca attttggctc atatgatgca tgatggaata actgccagtc gttttaaaga
     1141 gttgcaggaa ccagagtgta ttattgtagc accaactcga gaattggtca accagattta
     1201 tttggaagcc agaaaatttt cttttgggac ttgtgtaaga gctgttgtta tatatggggg
     1261 aacccagctg ggacattcaa ttcgacaaat agtacaaggc tgtaatatat tatgtgctac
     1321 tcctggaaga ctgatggata tcataggcaa agaaaagatt ggtctcaaac agatcaaata
     1381 cttagttttg gatgaagctg atcgcatgtt ggatatgggt tttggtccag aaatgaagaa
     1441 gttaatttct tgcccaggaa tgccatcaaa ggaacagcgc caaaccctta tgttcagtgc
     1501 aacttttcca gaggaaattc aaaggttggc tgcagagttt ttaaagtcaa attatctgtt
     1561 tgttgctgtt ggacaagtgg gtggagcatg tagagatgtt cagcagaccg ttctccaagt
     1621 tggccagttc tcaaaaagag aaaagctcgt tgaaattctg cgaaacatag gggatgaaag
     1681 aactatggtc tttgttgaaa ctaagaaaaa agcagatttt attgcaactt ttctttgtca
     1741 agaaaaaata tcaactacaa gtattcatgg tgatcgggaa cagagagagc gggagcaagc
     1801 tcttggagat tttcgctttg gaaagtgccc agttcttgtt gctacttcag tagctgccag
     1861 agggctggat attgaaaatg tgcaacatgt tatcaatttt gatcttcctt ctaccattga
     1921 tgaatatgtt catcgaattg ggcgtactgg tcgttgtggg aatactggca gagcaatttc
     1981 cttttttgat cttgaatcgg ataaccattt agcacagcct ctagtaaaag tattgacaga
     2041 tgctcaacag gatgttcctg catggttgga agaaattgcc tttagtacat acattcctgg
     2101 cttcagtggt agtacaagag gaaacgtgtt tgcatcagtt gataccagaa agggcaagag
     2161 cactttgaac acagctgggt tttcttcttc acaagctccc aatccagtag atgatgagtc
     2221 atgggattaa agccaaaaca tccttcaagt ctgtggtttt gatgcagaga agaaaatagt
     2281 tttgattttt gagtttttaa cagaagtata aaacttaaca ttctcatagc tcctgtcctt
     2341 gtattctcac tcctacactt aaaaaaaaaa tccttactga ctagttatgt gagatgctaa
     2401 aacttacaac attgcagtta ctgatacaaa tggtgttaac tgggaatatt aaagcattct
     2461 aaatgtcttt cttatttctg gtatattctt tagggggctt agacatgttt aatgtttaaa
     2521 tgccaagtct tactatagtg tttattgatc ttataaaaca agcaaatagg atatgataca
     2581 cttttggtta aaaattactg ggtctcattt ttacttgagt ctttaaaaca gtagtgtgtc
     2641 actataatgt gataatcttt aagagaaagt agaatactta agcctttcaa agtgattttg
     2701 atttttagat catcagatgt atgatgaaaa tggttaaatg tttgtgatgg gagctctgta
     2761 ctcaatggca taacaatgtt tatttttata atatacaatc tttccttgaa ataaaggatg
     2821 aaacactttt cccttaagtt ttccatc
//
