LOCUS       NP_060850                540 aa            linear   PRI 29-APR-2025
DEFINITION  probable ATP-dependent RNA helicase DDX28 [Homo sapiens].
ACCESSION   NP_060850
VERSION     NP_060850.2
DBSOURCE    REFSEQ: accession NM_018380.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 540)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (residues 1 to 540)
  AUTHORS   Evagelou,S.L., Bebenek,O., Specker,E.J. and Uniacke,J.
  TITLE     DEAD Box Protein Family Member DDX28 Is a Negative Regulator of
            Hypoxia-Inducible Factor 2alpha- and Eukaryotic Initiation Factor
            4E2-Directed Hypoxic Translation
  JOURNAL   Mol Cell Biol 40 (6), e00610-19 (2020)
   PUBMED   31907278
  REMARK    GeneRIF: The authors have identified the DEAD box protein family
            member DDX28 as an interactor and negative regulator of HIF-2alpha
            that suppresses HIF-2alpha's ability to activate eIF4E2-directed
            translation.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 540)
  AUTHORS   Antonicka,H. and Shoubridge,E.A.
  TITLE     Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA
            Processing and Ribosome Biogenesis
  JOURNAL   Cell Rep 10 (6), 920-932 (2015)
   PUBMED   25683715
REFERENCE   4  (residues 1 to 540)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   5  (residues 1 to 540)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (residues 1 to 540)
  AUTHORS   Bogenhagen,D.F., Rousseau,D. and Burke,S.
  TITLE     The layered structure of human mitochondrial DNA nucleoids
  JOURNAL   J Biol Chem 283 (6), 3665-3675 (2008)
   PUBMED   18063578
REFERENCE   7  (residues 1 to 540)
  AUTHORS   Valgardsdottir,R. and Prydz,H.
  TITLE     Transport signals and transcription-dependent nuclear localization
            of the putative DEAD-box helicase MDDX28
  JOURNAL   J Biol Chem 278 (23), 21146-21154 (2003)
   PUBMED   12663657
  REMARK    GeneRIF: Three domains in the N-terminal half of the protein and
            its RNA binding ability were essential for nucleolar localization
            as well as transcription-inhibition-dependent localization to
            nuclear subcompartments and two were the nuclear localization
            signal
REFERENCE   8  (residues 1 to 540)
  AUTHORS   Valgardsdottir,R., Brede,G., Eide,L.G., Frengen,E. and Prydz,H.
  TITLE     Cloning and characterization of MDDX28, a putative dead-box
            helicase with mitochondrial and nuclear localization
  JOURNAL   J Biol Chem 276 (34), 32056-32063 (2001)
   PUBMED   11350955
REFERENCE   9  (residues 1 to 540)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (residues 1 to 540)
  AUTHORS   Loftus,B.J., Kim,U.J., Sneddon,V.P., Kalush,F., Brandon,R.,
            Fuhrmann,J., Mason,T., Crosby,M.L., Barnstead,M., Cronin,L.,
            Deslattes Mays,A., Cao,Y., Xu,R.X., Kang,H.L., Mitchell,S.,
            Eichler,E.E., Harris,P.C., Venter,J.C. and Adams,M.D.
  TITLE     Genome duplications and other features in 12 Mb of DNA sequence
            from human chromosome 16p and 16q
  JOURNAL   Genomics 60 (3), 295-308 (1999)
   PUBMED   10493829
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC130462.3.
            
            On Aug 28, 2009 this sequence version replaced NP_060850.1.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure, such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of the DEAD box
            protein family are believed to be involved in embryogenesis,
            spermatogenesis, and cellular growth and division. This gene is
            intronless. It encodes an RNA-dependent ATPase. The encoded protein
            is localized in the mitochondria and the nucleus, and can be
            transported between the mitochondria and the nucleus. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR1660803.146912.1, BC024273.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000332395.7/
                                                  ENSP00000332340.6
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     Protein         1..540
                     /product="probable ATP-dependent RNA helicase DDX28"
                     /EC_number="3.6.4.13"
                     /note="mitochondrial DEAD-box polypeptide 28; DEAD/H
                     (Asp-Glu-Ala-Asp/His) box polypeptide 28; mitochondrial
                     DEAD box protein 28; DEAD (Asp-Glu-Ala-Asp) box
                     polypeptide 28"
                     /calculated_mol_wt=59450
     Region          3..18
                     /region_name="Mitochondrial targeting signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NUL7.2)"
     Region          126..156
                     /region_name="Q motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NUL7.2)"
     Region          137..371
                     /region_name="DEADc_DDX28"
                     /note="DEAD-box helicase domain of DEAD box protein 28;
                     cd17948"
                     /db_xref="CDD:350706"
     Site            order(147..148,150..152,155,173..180)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350706"
     Region          180..191
                     /region_name="Nuclear export signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NUL7.2)"
     Region          286..289
                     /region_name="DEAD"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NUL7.2)"
     Region          365..503
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(460,462,487,490..491)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          520..523
                     /region_name="Nuclear localization signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NUL7.2)"
     CDS             1..540
                     /gene="DDX28"
                     /gene_synonym="MDDX28"
                     /coded_by="NM_018380.4:31..1653"
                     /db_xref="CCDS:CCDS10858.1"
                     /db_xref="GeneID:55794"
                     /db_xref="HGNC:HGNC:17330"
                     /db_xref="MIM:607618"
ORIGIN      
        1 maltrpvrlf slvtrlllap rrgltvrspd eplpvvripv alqrqleqrq srrrnlprpv
       61 lvrpgpllvs arrpelnqpa rltlgrwera plasqgwksr rarrdhfsie raqqeapavr
      121 klsskgsfad lgleprvlha lqeaapevvq pttvqsstip sllrgrhvvc aaetgsgktl
      181 syllpllqrl lgqpsldslp ipaprglvlv psrelaqqvr avaqplgrsl gllvrdlegg
      241 hgmrrirlql srqpsadvlv atpgalwkal ksrlisleql sflvldeadt lldesflelv
      301 dyilekshia egpadledpf npkaqlvlvg atfpegvgql lnkvaspdav ttitssklhc
      361 imphvkqtfl rlkgadkvae lvhilkhrdr aertgpsgtv lvfcnssstv nwlgyilddh
      421 kiqhlrlqgq mpalmrvgif qsfqkssrdi llctdiasrg ldstgvelvv nydfpptlqd
      481 yihragrvgr vgsevpgtvi sfvthpwdvs lvqkielaar rrrslpglas svkeplpqat
//
