LOCUS       NM_017895               2570 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 2,
            mRNA.
ACCESSION   NM_017895
VERSION     NM_017895.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Li,G., Li,R., Wang,W., Sun,M. and Wang,X.
  TITLE     DDX27 regulates oral squamous cell carcinoma development through
            targeting CSE1L
  JOURNAL   Life Sci 340, 122479 (2024)
   PUBMED   38301874
  REMARK    GeneRIF: DDX27 regulates oral squamous cell carcinoma development
            through targeting CSE1L.
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Li,S., Ma,J., Zheng,A., Song,X., Chen,S. and Jin,F.
  TITLE     DEAD-box helicase 27 enhances stem cell-like properties with poor
            prognosis in breast cancer
  JOURNAL   J Transl Med 19 (1), 334 (2021)
   PUBMED   34362383
  REMARK    GeneRIF: DEAD-box helicase 27 enhances stem cell-like properties
            with poor prognosis in breast cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2570)
  AUTHORS   Xiaoqian,W., Bing,Z., Yangwei,L., Yafei,Z., Tingting,Z., Yi,W.,
            Qingjun,L., Suxia,L., Ling,Z., Bo,W. and Peng,Z.
  TITLE     DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma Progression
            Through ERK Signaling
  JOURNAL   Technol Cancer Res Treat 20, 15330338211055953 (2021)
   PUBMED   34855554
  REMARK    GeneRIF: DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma
            Progression Through ERK Signaling.
REFERENCE   4  (bases 1 to 2570)
  AUTHORS   Tang,J., Chen,H., Wong,C.C., Liu,D., Li,T., Wang,X., Ji,J.,
            Sung,J.J., Fang,J.Y. and Yu,J.
  TITLE     DEAD-box helicase 27 promotes colorectal cancer growth and
            metastasis and predicts poor survival in CRC patients
  JOURNAL   Oncogene 37 (22), 3006-3021 (2018)
   PUBMED   29535419
  REMARK    GeneRIF: Data found that DDX27 expression is significantly
            up-regulated in colorectal cancer (CRC) at mRNA and protein levels.
            DDX27 mRNA overexpression was positively correlated with DNA copy
            number gain and associated with poor prognosis in CRC patients.
            Further evidences prove that DDX27 plays a pivotal oncogenic role
            in CRC.
REFERENCE   5  (bases 1 to 2570)
  AUTHORS   Tsukamoto,Y., Fumoto,S., Noguchi,T., Yanagihara,K., Hirashita,Y.,
            Nakada,C., Hijiya,N., Uchida,T., Matsuura,K., Hamanaka,R.,
            Murakami,K., Seto,M., Inomata,M. and Moriyama,M.
  TITLE     Expression of DDX27 contributes to colony-forming ability of
            gastric cancer cells and correlates with poor prognosis in gastric
            cancer
  JOURNAL   Am J Cancer Res 5 (10), 2998-3014 (2015)
   PUBMED   26693055
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2570)
  AUTHORS   Kellner,M., Rohrmoser,M., Forne,I., Voss,K., Burger,K., Muhl,B.,
            Gruber-Eber,A., Kremmer,E., Imhof,A. and Eick,D.
  TITLE     DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S
            RNA and stably associates with the PeBoW-complex
  JOURNAL   Exp Cell Res 334 (1), 146-159 (2015)
   PUBMED   25825154
  REMARK    GeneRIF: The DDX27 can interact specifically with the Pes1 and Bop1
            but fulfils critical function(s) for proper 3' end formation of 47S
            rRNA independently of the PeBoW-complex.
REFERENCE   7  (bases 1 to 2570)
  AUTHORS   Zhou,J., Yong,W.P., Yap,C.S., Vijayaraghavan,A., Sinha,R.A.,
            Singh,B.K., Xiu,S., Manesh,S., Ngo,A., Lim,A., Ang,C., Xie,C.,
            Wong,F.Y., Lin,S.J., Wan,W.K., Tan,I.B., Flotow,H., Tan,P.,
            Lim,K.H., Yen,P.M. and Goh,L.K.
  TITLE     An integrative approach identified genes associated with drug
            response in gastric cancer
  JOURNAL   Carcinogenesis 36 (4), 441-451 (2015)
   PUBMED   25742747
  REMARK    GeneRIF: Levels of DDX27 mRNA and protein were increased in
            early-stage gastric tumors, and may be a potential diagnostic and
            prognostic marker for Gastric cancer.
REFERENCE   8  (bases 1 to 2570)
  AUTHORS   Ohta,S., Bukowski-Wills,J.C., Sanchez-Pulido,L., Alves Fde,L.,
            Wood,L., Chen,Z.A., Platani,M., Fischer,L., Hudson,D.F.,
            Ponting,C.P., Fukagawa,T., Earnshaw,W.C. and Rappsilber,J.
  TITLE     The protein composition of mitotic chromosomes determined using
            multiclassifier combinatorial proteomics
  JOURNAL   Cell 142 (5), 810-821 (2010)
   PUBMED   20813266
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL357560.11 and AL049766.14.
            
            On Jul 17, 2020 this sequence version replaced NM_017895.7.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this family
            are believed to be involved in embryogenesis, spermatogenesis, and
            cellular growth and division. This gene encodes a DEAD box protein
            involved in the processing of 5.8S and 28S ribosomal RNAs. More
            specifically, the encoded protein localizes to the nucleolus, where
            it interacts with the PeBoW complex to ensure proper 3' end
            formation of 47S rRNA. [provided by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK300135.1, BC011927.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618172.5/ ENSP00000482680.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               AL357560.11        25752-25877
            127-273             AL357560.11        27788-27934
            274-333             AL049766.14        207-266
            334-499             AL049766.14        518-683
            500-546             AL049766.14        2195-2241
            547-633             AL049766.14        2363-2449
            634-739             AL049766.14        3680-3785
            740-913             AL049766.14        5965-6138
            914-1064            AL049766.14        7449-7599
            1065-1164           AL049766.14        10556-10655
            1165-1306           AL049766.14        10818-10959
            1307-1460           AL049766.14        12185-12338
            1461-1542           AL049766.14        13400-13481
            1543-1720           AL049766.14        13583-13760
            1721-1827           AL049766.14        16199-16305
            1828-1930           AL049766.14        16486-16588
            1931-2025           AL049766.14        19143-19237
            2026-2149           AL049766.14        19333-19456
            2150-2237           AL049766.14        19844-19931
            2238-2312           AL049766.14        20879-20953
            2313-2570           AL049766.14        21066-21323
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..2570
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /note="DEAD-box helicase 27"
                     /db_xref="GeneID:55661"
                     /db_xref="HGNC:HGNC:15837"
                     /db_xref="MIM:616621"
     exon            1..126
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..2331
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /EC_number="3.6.4.13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27; probable
                     ATP-dependent RNA helicase DDX27; RNA helicase-like
                     protein; DEAD (Asp-Glu-Ala-Asp) box polypeptide 27; DEAD
                     box protein 27; Deficiency of ribosomal subunits protein 1
                     homolog"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX27
                     isoform 2"
                     /protein_id="NP_060365.8"
                     /db_xref="CCDS:CCDS13416.2"
                     /db_xref="GeneID:55661"
                     /db_xref="HGNC:HGNC:15837"
                     /db_xref="MIM:616621"
                     /translation="MLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRRQKALG
                     KNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDK
                     EAKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTLKV
                     KDRKKKKKKGQEAGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACI
                     PVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAPVTRVLVLVPTRELGIQVHS
                     VTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSI
                     EVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRI
                     FVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHI
                     LLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFT
                     MPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVIL
                     KFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQT
                     KEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAER
                     LAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRAGPSFEE
                     RKQLGLPHQRRGGNFKSKSRYKRRK"
     exon            127..273
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            274..333
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            334..499
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            500..546
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            547..633
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            634..739
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            740..913
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1064
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1164
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1306
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1460
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1542
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1720
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1827
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1930
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2025
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2149
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2237
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2312
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2570
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2539..2544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /note="hexamer: ATTAAA"
     polyA_site      2570
                     /gene="DDX27"
                     /gene_synonym="dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241;
                     RHLP"
                     /note="major polyA site"
ORIGIN      
        1 gtgctcgctt ccggaagtgg cttctgcgac aacatgcttg cggacctcgg cttaatcgga
       61 accataggcg aggatgacga ggtgccggtg gagcccgagt ctgactccgg ggacgaggaa
      121 gaggaggggc ccattgtgct gggcagacga caaaaagctt tggggaagaa ccgcagtgct
      181 gatttcaacc ctgatttcgt tttcactgag aaggagggga cgtacgatgg cagctgggcc
      241 ctggctgatg tcatgagcca actcaagaag aagagggcag ccactacatt agatgagaag
      301 attgagaaag ttcgaaagaa aaggaaaaca gaggataaag aagccaagtc tgggaagttg
      361 gaaaaggaga aagaagcaaa ggaaggctct gaaccaaagg agcaggaaga ccttcaagag
      421 aatgatgagg aaggctcaga agatgaagcc tcggagactg actactcatc agctgatgag
      481 aacatcctca ccaaagcaga tacactcaaa gtaaaggatc ggaagaagaa gaagaagaaa
      541 ggacaggaag caggaggatt ttttgaagat gcatctcagt acgatgaaaa cctctcgttc
      601 caggacatga acctttcccg ccctcttctg aaggccatta cagccatggg cttcaagcag
      661 cccaccccga tccagaaggc gtgcatacct gtgggtctat tggggaagga catctgtgcc
      721 tgtgcagcca ctgggacagg taaaactgcc gcctttgccc tgcctgtttt ggagcgtctg
      781 atttataaac cccgccaggc tccagtcacc cgcgtgctgg tgctagtgcc cacccgagag
      841 ctgggcatcc aggtgcactc tgtcaccaga cagctggccc agttctgcaa catcaccacc
      901 tgcctggctg tgggcggctt ggatgtgaag tctcaggaag cagctcttcg ggcagcgcct
      961 gacatcctca tcgccacccc aggccggctc atcgatcacc tccacaactg cccttccttc
     1021 cacctgagca gcatcgaggt gctcatcctg gacgaggctg acaggatgct ggatgagtac
     1081 tttgaggagc agatgaagga gatcatccga atgtgttccc accaccgcca gaccatgctc
     1141 ttctcggcca ccatgacaga cgaggtgaaa gatctggctt ctgtctcctt gaagaatcct
     1201 gtccggatat ttgtgaacag caacacagat gtggctccct tcctgcggca ggagttcatc
     1261 cggatccggc ctaatcgtga aggagaccgg gaagccatcg tggcagcttt gttgacgagg
     1321 accttcactg accatgtgat gctgttcacg caaaccaaga agcaggccca ccgcatgcac
     1381 atcctcctgg ggctcatggg gctgcaggtg ggtgagctcc atggcaactt gtcacagacg
     1441 cagcggctgg aggccctccg gcgttttaag gatgaacaga ttgacatcct cgtggccact
     1501 gatgtggcag cccgtggact tgacattgag ggggtcaaaa cggtaatcaa cttcacaatg
     1561 cctaatacca tcaaacatta tgtccaccgg gtggggcgaa cagcacgtgc tggcagggct
     1621 gggcgctcag tctctctggt gggagaagat gagcggaaga tgctgaagga gattgtaaaa
     1681 gctgccaagg cccctgtgaa ggccaggata cttccccaag atgtcatcct caaattccgg
     1741 gacaagattg agaaaatgga gaaagatgtg tatgcagttc tgcagctaga ggcggaggaa
     1801 aaagagatgc agcagtcaga agcccagatc aatacagcaa agcggctcct ggagaagggg
     1861 aaggaggcag tggtccaaga gcccgagagg agctggttcc agaccaaaga agagaggaag
     1921 aaggagaaaa ttgccaaagc tctgcaggaa tttgacttgg ccttaagagg aaagaagaaa
     1981 aggaagaagt ttatgaagga tgccaaaaaa aagggggaga tgacagcaga ggaaaggtct
     2041 cagtttgaaa tcctcaaggc gcagatgttt gctgaacggc tagcgaagag gaatcgcaga
     2101 gccaagcggg cccgagcaat gcccgaggag gagccagtga gaggtcctgc caagaagcaa
     2161 aagcagggga agaaatctgt atttgatgaa gaactcacca acacaagcaa gaaggccctg
     2221 aaacagtatc gagctggccc ttcctttgaa gaaaggaaac agttgggctt gccccaccag
     2281 agacgaggag gaaactttaa atctaaatcc agatacaaga ggaggaagta gctgtcgtgg
     2341 cctgaagaaa ttcatggggg cagcccttaa atcccttccc tgtgggaagt catcctggct
     2401 ggtctgtctt ttctccattt gtttaaaaaa aaaacaaaaa caaaaaacaa cactttggtg
     2461 tggtggtatg gtacgtagct attttcctaa gcatgtctgt caatctccct tcttgctgat
     2521 tagctttcat atgactatat taaatggaag tatttttggg aaaagagaaa
//
