LOCUS       NM_013264               7424 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens DEAD-box helicase 25 (DDX25), transcript variant 1,
            mRNA.
ACCESSION   NM_013264
VERSION     NM_013264.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Kavarthapu,R., Anbazhagan,R., Raju,M., Morris,C.T., Pickel,J. and
            Dufau,M.L.
  TITLE     Targeted knock-in mice with a human mutation in GRTH/DDX25 reveals
            the essential role of phosphorylated GRTH in spermatid development
            during spermatogenesis
  JOURNAL   Hum Mol Genet 28 (15), 2561-2572 (2019)
   PUBMED   31009948
  REMARK    GeneRIF: Targeted knock-in mice with a human mutation in GRTH/DDX25
            reveals the essential role of phosphorylated GRTH in spermatid
            development during spermatogenesis.
REFERENCE   3  (bases 1 to 7424)
  AUTHORS   Raju,M., Hassan,S.A., Kavarthapu,R., Anbazhagan,R. and Dufau,M.L.
  TITLE     Characterization of the Phosphorylation Site of GRTH/DDX25 and
            Protein Kinase A Binding Interface Provides Structural Basis for
            the Design of a Non-Hormonal Male Contraceptive
  JOURNAL   Sci Rep 9 (1), 6705 (2019)
   PUBMED   31040297
  REMARK    GeneRIF: Characterization of the Phosphorylation Site of GRTH/DDX25
            and Protein Kinase A Binding Interface Provides Structural Basis
            for the Design of a Non-Hormonal Male Contraceptive.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7424)
  AUTHORS   Jaiswal,D., Trivedi,S., Agrawal,N.K. and Singh,K.
  TITLE     Association of the gonadotrophin-regulated testicular RNA helicase
            gene polymorphism with human male infertility
  JOURNAL   Andrologia 46 (9), 1063-1066 (2014)
   PUBMED   24168058
  REMARK    GeneRIF: Analysed the association of GRTH gene IVS6+55G/T and
            c.852C/T polymorphisms with male infertility. The c.852 T allele
            was associated with an increased risk of male infertility (OR:
            3.16, P = 0.008), whereas IVS6+55G/T allele conferred no risk.
REFERENCE   5  (bases 1 to 7424)
  AUTHORS   Aston,K.I., Krausz,C., Laface,I., Ruiz-Castane,E. and Carrell,D.T.
  TITLE     Evaluation of 172 candidate polymorphisms for association with
            oligozoospermia or azoospermia in a large cohort of men of European
            descent
  JOURNAL   Hum Reprod 25 (6), 1383-1397 (2010)
   PUBMED   20378615
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 7424)
  AUTHORS   Tsai-Morris,C.H., Koh,E., Sheng,Y., Maeda,Y., Gutti,R., Namiki,M.
            and Dufau,M.L.
  TITLE     Polymorphism of the GRTH/DDX25 gene in normal and infertile
            Japanese men: a missense mutation associated with loss of GRTH
            phosphorylation
  JOURNAL   Mol Hum Reprod 13 (12), 887-892 (2007)
   PUBMED   17848414
  REMARK    GeneRIF: Data show that the Arg(242)His missense mutation of the
            GRTH/DDX25 gene associated with expression of a protein with
            reduced basicity, and the absence of the phospho-GRTH species,
            could impact on germ cell development and/or function.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 7424)
  AUTHORS   A,Z., Zhang,S., Yang,Y., Ma,Y., Lin,L. and Zhang,W.
  TITLE     Single nucleotide polymorphisms of the gonadotrophin-regulated
            testicular helicase (GRTH) gene may be associated with the human
            spermatogenesis impairment
  JOURNAL   Hum Reprod 21 (3), 755-759 (2006)
   PUBMED   16293649
  REMARK    GeneRIF: SNP IVS6+55G-->T and c.852C-->T of GRTH gene may be
            associated with male infertility with azoospermia or severe
            oligozoospermia.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 7424)
  AUTHORS   Tsai-Morris,C.H., Lei,S., Jiang,Q., Sheng,Y. and Dufau,M.L.
  TITLE     Genomic organization and transcriptional analysis of
            gonadotropin-regulated testicular RNA helicase--GRTH/DDX25 gene
  JOURNAL   Gene 331, 83-94 (2004)
   PUBMED   15094194
REFERENCE   9  (bases 1 to 7424)
  AUTHORS   Tsai-Morris,C.H., Sheng,Y., Lee,E., Lei,K.J. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is
            essential for spermatid development and completion of
            spermatogenesis
  JOURNAL   Proc Natl Acad Sci U S A 101 (17), 6373-6378 (2004)
   PUBMED   15096601
REFERENCE   10 (bases 1 to 7424)
  AUTHORS   Tang,P.Z., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     A novel gonadotropin-regulated testicular RNA helicase. A new
            member of the dead-box family
  JOURNAL   J Biol Chem 274 (53), 37932-37940 (1999)
   PUBMED   10608860
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC050360.1, AL563479.3,
            AF155140.3 and AP000842.6.
            
            On Nov 23, 2018 this sequence version replaced NM_013264.4.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure, such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of the DEAD box
            protein family are believed to be involved in embryogenesis,
            spermatogenesis, and cellular growth and division. This gene
            encodes a member of this family. The encoded protein is a
            gonadotropin-regulated and developmentally expressed testicular RNA
            helicase. It may serve to maintain testicular functions related to
            steroidogenesis and spermatogenesis. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.234420.1,
                                           SRR1803617.138928.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263576.11/ ENSP00000263576.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1203              BC050360.1         13-1215
            1204-1211           AL563479.3         459-466             c
            1212-1678           BC050360.1         1224-1690
            1679-1691           AF155140.3         1679-1691
            1692-7424           AP000842.6         85376-91108
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.2"
     gene            1..7424
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /note="DEAD-box helicase 25"
                     /db_xref="GeneID:29118"
                     /db_xref="HGNC:HGNC:18698"
                     /db_xref="MIM:607663"
     exon            1..73
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1462
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25;
                     gonadotropin-regulated testicular RNA helicase;
                     ATP-dependent RNA helicase DDX25; DEAD (Asp-Glu-Ala-Asp)
                     box polypeptide 25; DEAD box protein 25; DEAD
                     (Asp-Glu-Ala-Asp) box helicase 25; epididymis secretory
                     sperm binding protein"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DDX25 isoform 1"
                     /protein_id="NP_037396.3"
                     /db_xref="CCDS:CCDS44766.1"
                     /db_xref="GeneID:29118"
                     /db_xref="HGNC:HGNC:18698"
                     /db_xref="MIM:607663"
                     /translation="MASLLWGGDAGAAESERLNSHFSNLSQPRKNLWGIKSTAVRNID
                     GSINNINEDDEEDVVDLAANSLLNKLIHQSLVESSHRVEVLQKDPSSPLYSVKTFEEL
                     RLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSR
                     VNALELFPQCLCLAPTYELALQTGRVVEQMGKFCVDVQVMYAIRGNRIPRGTDITKQI
                     IIGTPGTVLDWCFKLKLIDLTKIRVFVLDEADVMIDTQGFSDHSIRIQRALPSECQML
                     LFSATFEDSVWHFAERIIPDPNVIKLRKEELTLNNIRQYYVLCEHRKDKYQALCNIYG
                     SITIGQAIIFCQTRRNAKWLTVEMIQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVL
                     ITTNVCARGIDVKQVTIVVNFDLPVKQGEEPDYETYLHRIGRTGRFGKKGLAFNMIEV
                     DELPSLMKIQDHFNSSIKQLNAEDMDEIEKIDY"
     exon            74..140
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            141..185
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            186..321
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            322..414
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            415..517
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            518..632
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            633..810
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1048
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1211
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1400
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..7424
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1658..1663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /note="hexamer: AATAAA"
     polyA_site      1691
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /note="major polyA site"
     regulatory      1963..1968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /note="hexamer: ATTAAA"
     polyA_site      1988
                     /gene="DDX25"
                     /gene_synonym="GRTH"
     regulatory      7392..7397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX25"
                     /gene_synonym="GRTH"
                     /note="hexamer: AATAAA"
     polyA_site      7424
                     /gene="DDX25"
                     /gene_synonym="GRTH"
ORIGIN      
        1 aatcgcagcc atggcgtcgt tactgtgggg aggcgacgca ggggcggcgg agagcgagcg
       61 gctgaacagc cacttttcaa acctcagcca accccggaag aacctttggg gtattaagag
      121 tactgcagtc cgaaacatag atggttctat taataacatc aatgaagatg atgaagaaga
      181 tgtagtggat ttggcagcta attcactctt aaacaagtta atccatcaat ccttagtaga
      241 atccagtcac cgtgtggaag ttttacagaa ggatcccagc tctccacttt actcagtaaa
      301 gacatttgaa gagctgcggc taaaggaaga gttactaaaa ggaatctatg caatgggatt
      361 taataggcca tctaaaatcc aagagatggc tctccctatg atgctggcac atccacccca
      421 gaacctcata gcacagagcc agtctggaac aggaaagaca gcggcatttg tgttggcaat
      481 gttaagcaga gttaatgcct tggaattgtt cccacagtgc ctctgcctag ctcctactta
      541 tgaattggct ctgcaaactg gccgtgtggt tgagcagatg ggaaaattct gtgtggatgt
      601 tcaagtgatg tatgccattc gagggaatcg aattcccaga ggcaccgaca tcactaaaca
      661 gattataatt ggcactcctg ggactgtcct agattggtgt tttaaactaa aattgattga
      721 tttgactaag attcgtgtgt ttgtcctgga tgaagcagat gtgatgattg acactcaagg
      781 attctcagat catagtattc gtattcaaag agctctaccc tccgaatgcc aaatgctcct
      841 cttttcagca acctttgagg actctgtgtg gcactttgct gagcgaatca tccctgaccc
      901 taatgttatc aagttacgca aagaggagct cacactgaac aacatccggc aatattacgt
      961 gctgtgtgag cacaggaaag acaaatacca agctctgtgc aacatttatg gcagcatcac
     1021 cattggtcag gccatcatct tctgccagac tcgtcgaaac gctaagtggt tgaccgtgga
     1081 gatgatacag gatggccacc aggtgtcttt gttaagcggg gagctgaccg tggagcagcg
     1141 agcttccatc attcagaggt ttcgggatgg gaaagagaag gttctcataa caactaatgt
     1201 ttgtgcccga gggattgatg tgaagcaggt cacaattgtt gtgaactttg atctccctgt
     1261 aaaacaagga gaggagccgg actatgagac ctacctccac cgcatagggc ggacggggcg
     1321 ctttgggaaa aaaggccttg ccttcaacat gattgaagta gatgagctgc cctcgctcat
     1381 gaaaatccag gaccacttta acagcagtat taagcaactc aacgctgaag acatggatga
     1441 aattgaaaag attgactatt gaagaaagaa ctttattgtt tgtgcagtat cctagtttat
     1501 gtgagaatgt ctcagtgggt tttgaaggta atttttttat gttgaggctt tttcgacgtg
     1561 aaactgtcac agatggcaaa ataaatgtca cggtacttag ttttaagaca tgaggtcttt
     1621 ttgaagccaa attgatgtga agcttgtgat ccttttgaat aaaaaaaagg caagattatt
     1681 tcttattcta atctttgtac aggtaatgtc tcaatgtggg ctatgggggt gtttttggat
     1741 ttggttgata caatctgagc agatcgtgcc tcttgggagc atcttactgt gtcacaagag
     1801 ggcggtagag cttcgctgat tttggaagcg gagcgagtag gaaaaagccc atgtcttcag
     1861 acctcactca caggtgccct ttccagccat ttatgccttt ccccgctccc cgccttaaac
     1921 tcccccatgc atgcacatgc atgttcttaa gtgaaagtac agattaaaac ttttaaaaag
     1981 tgaaaccatt tgaaagaaag agtattttac ttctttaatg tttcaaggga catacagaat
     2041 ttcagagtat ttttctattt tgcttctctt caaattaaag atttactccc tcatcagtta
     2101 tttcatagtc tctaaaagtg tggctgtatt atgggcattt cttaaaagca gtcgggaggg
     2161 taaaaaaaaa acccacatac cctattaaag aagtaacatt ttagagagca aaagtttttt
     2221 gcttttaaga atttaaaggt atgtcagaat cctggacatt ttcccacaag acccattgag
     2281 atacttttta atttttcata tttcctactc aaaagtaaag tgtagtgagc ttgagaagat
     2341 ggggtaaatg gagagtcccc cagaaagccg gagcggatct tgtaaccaga gcgccggtac
     2401 cagctctgtc acggcaccaa actgcaccag tggctgcagt gagtggccct gtcatcggtg
     2461 gcatggtggt tcctggcata tctattttga tatccctctt ccatgtcacc ctgcccatgt
     2521 tttctcccgg agagcagggc tcagaaatac ataaaaatca tcacgtggct gggcacagtg
     2581 gctcacacct gtaatcctag cactttggga ggccaaggag ggcggatcac gaggtcagga
     2641 gttaagacca gccttaccaa catggtgaaa ccccttctct actaaaaata taaaaattag
     2701 ctgggcgtgg tggtacgcgt gcctgtaatc ccagctactc aggaggctga ggcaggagaa
     2761 tcacttgaac ccaggaggcc gagattgcag tgagccgaga tcgtgccact gcattccagc
     2821 ctgggcgaca gagtgagact ctatctcaaa aaaaacgcgc tcccctccct catttagcaa
     2881 cagatattta ttgtataccc tttatgccaa acaccaggga aaaggaggtg agtgagatac
     2941 aacagccatg ttggaattca gagcctaatg gcagagacct agaataagcc agcatgataa
     3001 cagactgtgc taaagcaagc gggcccagat cactagtgta gcacagagga gggaccctaa
     3061 caccatctag gctggctccc agaggagagg acactcagga tctcctacag gagagcagga
     3121 tgtattcaac tgtgccttct cagcatggac tttgtaccac ccagcaactt tctaacctga
     3181 gtctacgaga ggaagtgttc cagacccagc ttaccataat tacctggaga gcccggggaa
     3241 aaaaatacag atttccttcc ccatgcagat gaaagcagac tctccgaggg cagagcccag
     3301 aaatacccac ttccataagg gagccgcaag tgcaggtaga gcagtggtaa cgaagatggc
     3361 cgtcccagcg ggagaccctc aggcagagag gcccttgggg acagaacttg taacacatgg
     3421 aactcctgct gctcggtgtc catcctggtt ctccttatcc tacatctgcc cttctctctg
     3481 gactggaagc tggtgatcac tcttgtccac tcaatacccc taacacagtg cccagtgcat
     3541 tctaagtgcc caaggaacct ttgtctttgt tgagtaaatt tttttttctt ttgccacttc
     3601 ctcattcact aataattttt ttttaggttt tccatcctag ttttgaaccc caattacatg
     3661 gcacaggatg aatgagcatg agaagtgtat ccagatactt tgttctctta ctattcgaga
     3721 atcgagttgg accttccacc gtgctcagct ccgccttggg gtgtcctaaa taaaacttta
     3781 ccactttcct ttacagttca aatctctggt aagatctccg ccagttaccc tcacaaatgt
     3841 cctcagtaat ttttgtttgg cagctgttcc cacaggtctg catgaaccag gtatttttct
     3901 gcgttccctt ttgccccctc ctcacgtccc tttgtctcac cacctaggag gcagcaaagc
     3961 catcctgtgt cacagctgca ttaacacgaa ctggctagtt tggtgagtca acaatccaaa
     4021 ggctgttttt tgcagggtgc agctcctgtc agagctgtaa gcaattttcc cgtcttcctg
     4081 catggcctgg ccaaggtcat ctctctcagt gcctgcctga ggacagagta gatagagagg
     4141 caaggaaaca ccaggtgatt tcagtgcaac tgtgagctgg gttcatcagc tgtatgtttc
     4201 ttggcaccaa atactaaacc aaatccgtta agtttatctt ggccagctta atactgttag
     4261 catattgaaa ttcatgatta cgtagagcag gactgtgatt tccagtgggt aagtgaacac
     4321 ctcgcatgga acacggctgc tatgatgtta ggtgctggat ctaaggcaac cattaataat
     4381 aggaaaggca aataagttgg tcatagtctg gtaggatgtg tgacagccgc ttttcaagag
     4441 acattattgc ttttggtcat gagaaagtgt tgatcctcct cagcaaagct cggtcaccat
     4501 gaaagctgct ggcagtctct gcacatatga ccaacagtgg aaaatcttag aggagtgatg
     4561 ggggtggtgg gtggtgggca gtacctttcc agacagcaga gagagagagg ctggttttgt
     4621 caagccttgg cttggttcct ggtcctctga gaggctcagt ctccttcctt ttgattcaag
     4681 gcaggaccta gattagaaag agaacccctg cagagacagt ccagctgttg ggatgggcct
     4741 gcactttgct ctctgaaccg gggggcatta ctatgttctc acccacattg tacagacagc
     4801 ccatcatgct cagccttgca ggagggtctt caggagccca gccatctggc acatactgct
     4861 tttcccaagg gagtaacaga aaggtcagat cctcttcaat cctaaccctc tcacttcaac
     4921 tctccactac ttctggggaa taatgatgta ttatccaaga ccctcatatc atattaaccc
     4981 acagactatg aaatcatcac agtcttgttc tatgctacag ggaatacgaa ctttttgccc
     5041 tagaaagcat ccaagaggca ctcaacccca acattttcta tacttttgct aatccgtggc
     5101 tgagagaatt tgacattttc ccttctcttt tttttttttt tgagatggag tctcactgtg
     5161 ttgcccaggc tggagtgcag tggcgtgatc tcggctcact gcaaactctg cctccccggt
     5221 tcaagcaatt ctcctgtctc agcctcccga gtagctgggg ctacaggcac atgccaccac
     5281 gcctggctta tttttgtatg ttattttagt agcgatgggg tttcaccgta ttggtcaggc
     5341 tggtctcgaa ctcgtgacct caggtgatcc acacgccttg gcttcccaaa gtgctgggat
     5401 tacaggcatc agccacggcg cccagcctcc ccttctcatt tctgtctgct gactcctctt
     5461 cccgttctgc tgctcacatc cgcaggttga tgttttcacc ctcggggttc tgccttatcc
     5521 cctgccaccc tcctgtgctg cctgggtccc tcagaaggat gggtctggac ttcccacacc
     5581 ctccttcgct gcccctcact gtgttgcctg gtgtctgcct gaagagatgc agtttgagcc
     5641 attggctggt ttctcttact gctttgcaaa ctatcagttt taaatgtggg tggggctgaa
     5701 gcaccattct ccatttttaa tgtggatttt ccatccccct ttaaacatta aacttttaga
     5761 ttcttctagt gaaatacaga aaacacacgt gaactgggat gattcttggc tgagagatgt
     5821 cccatggtgt ttggcaaacc cacggagggg cccacaggcc catgtctgga actgcctggc
     5881 cctcctttac cagtttatca gccttccatc tctgctgagg tagaaacagc cagaggctct
     5941 gatataataa agatgagctc tgaagagggg ctctctctcc ctcttgtatt cagaagaact
     6001 gaaagttttc ataagtctct agagaagcgt ggcaactatc tgtagcattt ttctttggga
     6061 attaaccttt gcccataatt atttctgttc agtagaataa ccatgggttt ctctattcca
     6121 gtttctagaa tataattttc ataaatgagg atggtctcct attttgagct taacattttt
     6181 ctattgctag aaagaatggt actgcctcac tccagctcct tagtgtgtga tcatggcttg
     6241 aaatctctgc tttctgttat attcggaggt aacatgtata ggtcatgtat aatggcctca
     6301 ggcatgattt agaaggcaca gactgagcag gctgctttgt gaggtggtga actccccatt
     6361 catgagagca ttcaatcaat cagaagatga atagttaatc agatgctgta gttctggttc
     6421 ttgaataacc aggagttcag aataaaatga tctcggagac tctgtccatt ctaagatacc
     6481 atgtttccat gaccctacac acaatagcta cacaggtgga gcaacatgag tctgagaagc
     6541 tttgcacaaa agaagattgt tttccacaaa tgaaaatggg gtggttaact tctaattgta
     6601 ccctgtctta gcgttcagat gcattggttc cttccccatg tgaccatctc ttcattcaaa
     6661 gcttaggctg aatgtggatt atttttaatt taatgttcat attataaaag caatgaaaca
     6721 gcaaagtaga gcaaaatatc aacaatccaa tgactttatc ataaaaatta tcagcttgat
     6781 atagtcctgt tcttaatata aacatctgtg gttaattata gtattatatc tttttatttg
     6841 aaacattcaa gttttttttc atgttaccac acagtgtttg ggattatatt ttcaaaataa
     6901 taattgacca gattgtccta tctagtgaat atgcatatag ctgactttgt tgaacattta
     6961 taggatgtcc atttttccaa agctttaatt ataaatcatg ctgcagtaaa tatcttctcg
     7021 cccaatattc cgaattattt cttaagatta gagacattaa gtgcgagtac caggtcgaaa
     7081 actagaagca ctttaacagc atttaacaga ttttgttaga gctacattgc attcagtaga
     7141 ctggctatac caatatatag ttgcacagca aattcacctc accttaccag caagggatat
     7201 tttcatttta aaattgcttg gtaacttttc tggtttatgt ttctcttagt acaagcaaga
     7261 ttatattttt taaagtggtt catcatttga aaagcatctg tagtgttctt tgcccattta
     7321 tcttgtggga tgttaatggt ttccttgtca agttgtgtga aatatatgtg tgaatttatt
     7381 tttccaaata aaataaagac attaaccttc tattttactg acaa
//
