LOCUS       NM_004818               3256 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.
ACCESSION   NM_004818
VERSION     NM_004818.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Segovia,D., Adams,D.W., Hoffman,N., Safaric Tepes,P., Wee,T.L.,
            Cifani,P., Joshua-Tor,L. and Krainer,A.R.
  TITLE     SRSF1 interactome determined by proximity labeling reveals direct
            interaction with spliceosomal RNA helicase DDX23
  JOURNAL   Proc Natl Acad Sci U S A 121 (21), e2322974121 (2024)
   PUBMED   38743621
  REMARK    GeneRIF: SRSF1 interactome determined by proximity labeling reveals
            direct interaction with spliceosomal RNA helicase DDX23.
            Erratum:[Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2412536121.
            doi: 10.1073/pnas.2412536121.. PMID: 39008678]
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Lin,C., Li,T., Wang,Y., Lai,S., Huang,Y., Guo,Z., Zhang,X. and
            Weng,S.
  TITLE     METTL3 enhances pancreatic ductal adenocarcinoma progression and
            gemcitabine resistance through modifying DDX23 mRNA N6 adenosine
            methylation
  JOURNAL   Cell Death Dis 14 (3), 221 (2023)
   PUBMED   36977668
  REMARK    GeneRIF: METTL3 enhances pancreatic ductal adenocarcinoma
            progression and gemcitabine resistance through modifying DDX23 mRNA
            N6 adenosine methylation.
            Erratum:[Cell Death Dis. 2023 Jul 31;14(7):485. doi:
            10.1038/s41419-023-05939-1.. PMID: 37524717]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3256)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 3256)
  AUTHORS   Burns,W., Bird,L.M., Heron,D., Keren,B., Ramachandra,D.,
            Thiffault,I., Del Viso,F., Amudhavalli,S., Engleman,K., Parenti,I.,
            Kaiser,F.J., Wierzba,J., Riedhammer,K.M., Liptay,S., Zadeh,N.,
            Porrmann,J., Fischer,A., Gosswein,S., McLaughlin,H.M.,
            Telegrafi,A., Langley,K.G., Steet,R., Louie,R.J. and Lyons,M.J.
  TITLE     Syndromic neurodevelopmental disorder associated with de novo
            variants in DDX23
  JOURNAL   Am J Med Genet A 185 (10), 2863-2872 (2021)
   PUBMED   34050707
  REMARK    GeneRIF: Syndromic neurodevelopmental disorder associated with de
            novo variants in DDX23.
REFERENCE   5  (bases 1 to 3256)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3256)
  AUTHORS   Irobi,J., Nelis,E., Verhoeven,K., De Vriendt,E., Dierick,I., De
            Jonghe,P., Van Broeckhoven,C. and Timmerman,V.
  TITLE     Mutation analysis of 12 candidate genes for distal hereditary motor
            neuropathy type II (distal HMN II) linked to 12q24.3
  JOURNAL   J Peripher Nerv Syst 7 (2), 87-95 (2002)
   PUBMED   12090300
REFERENCE   7  (bases 1 to 3256)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   8  (bases 1 to 3256)
  AUTHORS   Achsel,T., Ahrens,K., Brahms,H., Teigelkamp,S. and Luhrmann,R.
  TITLE     The human U5-220kD protein (hPrp8) forms a stable RNA-free complex
            with several U5-specific proteins, including an RNA unwindase, a
            homologue of ribosomal elongation factor EF-2, and a novel WD-40
            protein
  JOURNAL   Mol Cell Biol 18 (11), 6756-6766 (1998)
   PUBMED   9774689
REFERENCE   9  (bases 1 to 3256)
  AUTHORS   Laggerbauer,B., Achsel,T. and Luhrmann,R.
  TITLE     The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in
            vitro
  JOURNAL   Proc Natl Acad Sci U S A 95 (8), 4188-4192 (1998)
   PUBMED   9539711
REFERENCE   10 (bases 1 to 3256)
  AUTHORS   Teigelkamp,S., Mundt,C., Achsel,T., Will,C.L. and Luhrmann,R.
  TITLE     The human U5 snRNP-specific 100-kD protein is an RS
            domain-containing, putative RNA helicase with significant homology
            to the yeast splicing factor Prp28p
  JOURNAL   RNA 3 (11), 1313-1326 (1997)
   PUBMED   9409622
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK128428.1, BC002366.2 and
            AF026402.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004818.2.
            
            Summary: This gene encodes a member of the DEAD box protein family.
            DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure, such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this family
            are believed to be involved in embryogenesis, spermatogenesis, and
            cellular growth and division. The protein encoded by this gene is a
            component of the U5 snRNP complex; it may facilitate conformational
            changes in the spliceosome during nuclear pre-mRNA splicing. An
            alternatively spliced transcript variant has been found for this
            gene, but its biological validity has not been determined.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF026402.1, SRR1660805.154689.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308025.8/ ENSP00000310723.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK128428.1         1-30
            31-3248             BC002366.2         1-3218
            3249-3256           AF026402.1         3209-3216
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.12"
     gene            1..3256
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /note="DEAD-box helicase 23"
                     /db_xref="GeneID:9416"
                     /db_xref="HGNC:HGNC:17347"
                     /db_xref="MIM:612172"
     exon            1..80
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..2543
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /EC_number="3.6.4.13"
                     /note="PRP28 homolog, yeast; DEAD (Asp-Glu-Ala-Asp) box
                     polypeptide 23; PRP28p homolog; U5 snRNP 100 kD protein;
                     DEAD box protein 23; 100 kDa U5 snRNP-specific protein; U5
                     snRNP-specific 100-kD protein"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX23"
                     /protein_id="NP_004809.2"
                     /db_xref="CCDS:CCDS8770.1"
                     /db_xref="GeneID:9416"
                     /db_xref="HGNC:HGNC:17347"
                     /db_xref="MIM:612172"
                     /translation="MAGELADKKDRDASPSKEERKRSRTPDRERDRDRDRKSSPSKDR
                     KRHRSRDRRRGGSRSRSRSRSKSAERERRHKERERDKERDRNKKDRDRDKDGHRRDKD
                     RKRSSLSPGRGKDFKSRKDRDSKKDEEDEHGDKKPKAQPLSLEELLAKKKAEEEAEAK
                     PKFLSKAEREAEALKRRQQEVEERQRMLEEERKKRKQFQDLGRKMLEDPQERERRERR
                     ERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEW
                     DASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKE
                     QEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRS
                     WKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL
                     VWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISR
                     EDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKI
                     LEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRP
                     AVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAK
                     SLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYD
                     MAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANH
                     PDAQHKPGTILTKKRREETIFA"
     exon            81..289
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            290..400
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            401..494
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            495..560
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            561..699
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            700..833
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            834..946
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1090
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1316
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1462
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1640
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1883
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2035
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2144
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2319
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..3256
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3229..3234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /note="hexamer: AATAAA"
     polyA_site      3256
                     /gene="DDX23"
                     /gene_synonym="prp28; PRPF28; SNRNP100; U5-100K; U5-100KD"
                     /note="major polyA site"
ORIGIN      
        1 atctccgcga ccaggaaacg ggaaagatgg cgacggctcc gcgacgttga ggccgcgttg
       61 ggcggttcag actcagggtg atggcaggag agctggctga caaaaaggac cgtgatgcat
      121 caccttccaa ggaggaaagg aagcgatcac ggactcctga cagagagcgg gatagagacc
      181 gggaccggaa gtcttcccca tctaaagata gaaagcggca tcgttcaagg gatagacgtc
      241 gaggaggcag ccgttctcgc tctcgttccc gttccaaatc tgcagaaaga gaacgacggc
      301 acaaagaacg agaacgagat aaggagcggg atcggaataa gaaggaccga gatcgagaca
      361 aggatgggca cagacgggac aaggaccgta aacgatccag cttatctcct ggtcgaggaa
      421 aagactttaa atctcggaag gacagagact ctaagaagga tgaagaggat gaacatggtg
      481 ataagaagcc taaggcccag ccattatccc tggaggagct tctggccaag aaaaaggctg
      541 aggaagaagc tgaggctaag cccaagttcc tctctaaagc agaacgagag gctgaagctc
      601 taaagcgacg gcagcaggag gtggaagagc ggcagaggat gcttgaagaa gagaggaaga
      661 aaaggaaaca gttccaagac ttgggcagga agatgttgga agatcctcag gaacgggaac
      721 gtcgggaacg cagggagagg atggaacggg agaccaatgg aaatgaggat gaggaagggc
      781 ggcagaagat ccgggaagag aaggataaga gcaaggaact gcatgccatt aaggagcgtt
      841 acctgggtgg catcaaaaag cggcgccgaa cgagacatct caatgaccgg aaatttgttt
      901 ttgagtggga tgcatctgag gacacatcca ttgactacaa ccccctgtac aaagaacggc
      961 accaggtgca gttgttaggg cgaggcttca ttgcaggcat tgacctcaag cagcagaagc
     1021 gagagcagtc acgtttctat ggagacctaa tggagaagag gcgaaccctg gaagaaaagg
     1081 agcaggagga ggcaagactc cgcaaacttc gtaagaagga agccaagcag cgctgggatg
     1141 atcgtcattg gtctcagaaa aagttagatg agatgacgga cagggactgg cggatcttcc
     1201 gtgaggacta cagcatcacc accaaaggtg gcaagatccc caatcccatc cgatcctgga
     1261 aagactcttc tctgccccca cacatcttgg aggtcattga taagtgtggc tacaaggaac
     1321 caacacctat acagcgtcag gcaattccca ttgggctaca gaatcgtgac atcattggtg
     1381 tggctgagac tggcagtggc aagacagcag ccttcctcat ccctctgctg gtctggatca
     1441 ccacacttcc caaaattgac aggatcgaag agtcagacca aggcccttat gccatcatcc
     1501 tggctcccac ccgtgagttg gctcaacaga ttgaggaaga gaccatcaag tttgggaaac
     1561 cgctaggtat ccgcactgtg gctgtcattg gtggcatctc cagagaagac cagggcttca
     1621 ggctgcgcat gggttgtgag attgtgattg ctacccctgg gcgtttgatt gatgtgctgg
     1681 agaaccgcta cctggtgctg agccgctgta cctatgtggt tctggatgag gcagatagga
     1741 tgattgacat gggctttgag ccagatgtcc agaagatcct ggagcacatg cctgtcagca
     1801 accagaagcc agacacggat gaggctgagg accctgagaa gatgctggcc aactttgagt
     1861 cgggaaaaca taagtaccgc caaacagtca tgttcacggc caccatgccc ccagcggtgg
     1921 agcgtctggc caggagctat cttcggcgac ctgctgtggt gtacattggc tccgcaggca
     1981 agccccatga gcgtgtggaa cagaaggtct tcctcatgtc agagtcagaa aagaggaaaa
     2041 agctgctggc aatcttggag caaggctttg acccacccat cattattttt gtcaaccaga
     2101 agaagggctg cgacgtgttg gccaaatccc tggagaagat ggggtacaat gcttgcacac
     2161 tgcacggtgg aaaaggccag gagcagcgag agtttgcgtt gtccaacctc aaggctgggg
     2221 ccaaggatat tttggtggct acagatgtgg ctggtcgtgg tattgacatc caagatgtgt
     2281 ctatggttgt caactatgat atggccaaaa atattgaaga ttacatccac cgcattggcc
     2341 gcacgggacg agcaggcaag agtggggtgg ccatcacctt cctcacaaaa gaggactctg
     2401 ctgtgttcta cgagctgaag caagctatcc tggaaagccc agtgtcttcc tgtccccccg
     2461 aactagccaa ccacccagat gcccagcata agccaggcac catcctcacc aagaagcgcc
     2521 gggaagagac catctttgcc tgacacagca ctcttcctgt gggctgaggg catctccaaa
     2581 gctgcctgat gcctgttttt cagaaccctc acatccctct ttccaggtcc tcactcttgg
     2641 gatatggggg cttaggaaaa caatccaact ccctagccca gaccctcagg tcaggaggcc
     2701 tgcgtgtggg gctgcaaaag gagaggacga cgctgtcgga ggcagggaga gcaaattacc
     2761 acagcttctt ggcccagttc tgcccttctt tgctttggga ttgcactggg ccatcagctc
     2821 atgccaggct atgggggcag ccagttggca ttgctcccca gactgaacag aaacctggcc
     2881 gccggatggg acctcctttg gcacagactt gactgtgtaa ctgcataaac tgcagtagca
     2941 tcattgccct agatgcccca ggagacctgg caccatgagg attacagaca gtggaatctt
     3001 actgtcatct ggacagctgt tttcctgttt ggatggtaaa ggaagttgag agtctttaga
     3061 cctgtgcaca gccccgcacc aaggggtgct gtatgctcta ggcatcccct cccccagggg
     3121 attttctaag tagatggggg gacacggtga actggctgtg tccatctttg tcactgagtg
     3181 aaatctctgt tttctattct ctgagaagat aagtttgtat gttctgagaa taaatacatg
     3241 aatattaaga ctgtta
//
