LOCUS       NM_015214               4682 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens DDHD domain containing 2 (DDHD2), transcript variant
            1, mRNA.
ACCESSION   NM_015214 XM_291291
VERSION     NM_015214.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Su,X., Liang,F., Zeng,Y., Yang,Z.R., Deng,Y.Z., Xu,Y.H. and
            Cai,X.W.
  TITLE     Radiation-Induced Endothelial Ferroptosis Accelerates
            Atherosclerosis via the DDHD2-Mediated Nrf2/GPX4 Pathway
  JOURNAL   Biomolecules 14 (7), 879 (2024)
   PUBMED   39062593
  REMARK    GeneRIF: Radiation-Induced Endothelial Ferroptosis Accelerates
            Atherosclerosis via the DDHD2-Mediated Nrf2/GPX4 Pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Jia,F., Wang,X., Fu,Y., Zhao,S.M., Lu,B. and Wang,C.
  TITLE     DDHD2, whose mutations cause spastic paraplegia type 54, enhances
            lipophagy via engaging ATG8 family proteins
  JOURNAL   Cell Death Differ 31 (3), 348-359 (2024)
   PUBMED   38332048
  REMARK    GeneRIF: DDHD2, whose mutations cause spastic paraplegia type 54,
            enhances lipophagy via engaging ATG8 family proteins.
REFERENCE   3  (bases 1 to 4682)
  AUTHORS   Sun,L., Yang,X., Khan,A., Yu,X., Zhang,H., Han,S., Habulieti,X.,
            Sun,Y., Wang,R. and Zhang,X.
  TITLE     Panoramic variation analysis of a family with neurodevelopmental
            disorders caused by biallelic loss-of-function variants in TMEM141,
            DDHD2, and LHFPL5
  JOURNAL   Front Med 18 (1), 81-97 (2024)
   PUBMED   37837560
  REMARK    GeneRIF: Panoramic variation analysis of a family with
            neurodevelopmental disorders caused by biallelic loss-of-function
            variants in TMEM141, DDHD2, and LHFPL5.
REFERENCE   4  (bases 1 to 4682)
  AUTHORS   Chou,Y.T., Hsu,S.L., Tsai,Y.S., Lu,Y.J., Yu,K.W., Wu,H.M.,
            Liao,Y.C. and Lee,Y.C.
  TITLE     Biallelic DDHD2 mutations in patients with adult-onset complex
            hereditary spastic paraplegia
  JOURNAL   Ann Clin Transl Neurol 10 (9), 1603-1612 (2023)
   PUBMED   37420318
  REMARK    GeneRIF: Biallelic DDHD2 mutations in patients with adult-onset
            complex hereditary spastic paraplegia.
REFERENCE   5  (bases 1 to 4682)
  AUTHORS   Morikawa,R.K., Aoki,J., Kano,F., Murata,M., Yamamoto,A.,
            Tsujimoto,M. and Arai,H.
  TITLE     Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor
            involved in coat protein complex I- and Rab6-independent retrograde
            transport between the endoplasmic reticulum and the Golgi complex
  JOURNAL   J Biol Chem 284 (39), 26620-26630 (2009)
   PUBMED   19632984
REFERENCE   6  (bases 1 to 4682)
  AUTHORS   Kwek,S.S., Roy,R., Zhou,H., Climent,J., Martinez-Climent,J.A.,
            Fridlyand,J. and Albertson,D.G.
  TITLE     Co-amplified genes at 8p12 and 11q13 in breast tumors cooperate
            with two major pathways in oncogenesis
  JOURNAL   Oncogene 28 (17), 1892-1903 (2009)
   PUBMED   19330026
  REMARK    GeneRIF: Data show that FGFR1 and DDHD2 at 8p12 cooperated
            functionally with MYC, whereas CCND1 and ZNF703 cooperated with a
            dominant negative form of TP53.
REFERENCE   7  (bases 1 to 4682)
  AUTHORS   Bernard-Pierrot,I., Gruel,N., Stransky,N., Vincent-Salomon,A.,
            Reyal,F., Raynal,V., Vallot,C., Pierron,G., Radvanyi,F. and
            Delattre,O.
  TITLE     Characterization of the recurrent 8p11-12 amplicon identifies
            PPAPDC1B, a phosphatase protein, as a new therapeutic target in
            breast cancer
  JOURNAL   Cancer Res 68 (17), 7165-7175 (2008)
   PUBMED   18757432
REFERENCE   8  (bases 1 to 4682)
  AUTHORS   Shimoi,W., Ezawa,I., Nakamoto,K., Uesaki,S., Gabreski,G.,
            Aridor,M., Yamamoto,A., Nagahama,M., Tagaya,M. and Tani,K.
  TITLE     p125 is localized in endoplasmic reticulum exit sites and involved
            in their organization
  JOURNAL   J Biol Chem 280 (11), 10141-10148 (2005)
   PUBMED   15623529
REFERENCE   9  (bases 1 to 4682)
  AUTHORS   Nakajima,K., Sonoda,H., Mizoguchi,T., Aoki,J., Arai,H.,
            Nagahama,M., Tagaya,M. and Tani,K.
  TITLE     A novel phospholipase A1 with sequence homology to a mammalian
            Sec23p-interacting protein, p125
  JOURNAL   J Biol Chem 277 (13), 11329-11335 (2002)
   PUBMED   11788596
REFERENCE   10 (bases 1 to 4682)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA412370.1, AK056525.1,
            AK125904.1, AB018268.1, BM510691.1 and AC087362.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_015214.2.
            
            Summary: This gene encodes a phospholipase enzyme containing
            sterile-alpha-motif (SAM), WWE, and DDHD domains. This protein
            participates in membrane trafficking between the endoplastic
            reticulum and the Golgi body. Mutations in this gene can cause
            autosomal recessive spastic paraplegia 54. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Dec
            2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Both variants 1 and
            2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.67252.1,
                                           SRR1803614.83561.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397166.7/ ENSP00000380352.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DA412370.1         95-98
            5-677               AK056525.1         1-673
            678-3530            AK125904.1         595-3447
            3531-4603           AB018268.1         2835-3907
            4604-4615           BM510691.1         15-26               c
            4616-4682           AC087362.13        22769-22835
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     gene            1..4682
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /note="DDHD domain containing 2"
                     /db_xref="GeneID:23259"
                     /db_xref="HGNC:HGNC:29106"
                     /db_xref="MIM:615003"
     exon            1..275
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            276..503
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..2419
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /EC_number="3.1.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sec23p-interacting protein p125-like phosphatidic
                     acid-preferring phospholipase A1; PA-PLA1 like;
                     phospholipase DDHD2; SAM, WWE and DDHD domain-containing
                     protein 1; intracellular phospholipase A1gamma; TAG
                     hydrolase; triglyceride lipase; triglyceride hydrolase
                     DDHD2; triacylglycerol hydrolase DDHD2"
                     /codon_start=1
                     /product="triacylglycerol hydrolase DDHD2 isoform 1"
                     /protein_id="NP_056029.2"
                     /db_xref="CCDS:CCDS34883.1"
                     /db_xref="GeneID:23259"
                     /db_xref="HGNC:HGNC:29106"
                     /db_xref="MIM:615003"
                     /translation="MSSVQSQQEQLSQSDPSPSPNSCSSFELIDMDAGSLYEPVSPHW
                     FYCKIIDSKETWIPFNSEDSQQLEEAYSSGKGCNGRVVPTDGGRYDVHLGERMRYAVY
                     WDELASEVRRCTWFYKGDKDNKYVPYSESFSQVLEETYMLAVTLDEWKKKLESPNREI
                     IILHNPKLMVHYQPVAGSDDWGSTPTEQGRPRTVKRGVENISVDIHCGEPLQIDHLVF
                     VVHGIGPACDLRFRSIVQCVNDFRSVSLNLLQTHFKKAQENQQIGRVEFLPVNWHSPL
                     HSTGVDVDLQRITLPSINRLRHFTNDTILDVFFYNSPTYCQTIVDTVASEMNRIYTLF
                     LQRNPDFKGGVSIAGHSLGSLILFDILTNQKDSLGDIDSEKDSLNIVMDQGDTPTLEE
                     DLKKLQLSEFFDIFEKEKVDKEALALCTDRDLQEIGIPLGPRKKILNYFSTRKNSMGI
                     KRPAPQPASGANIPKESEFCSSSNTRNGDYLDVGIGQVSVKYPRLIYKPEIFFAFGSP
                     IGMFLTVRGLKRIDPNYRFPTCKGFFNIYHPFDPVAYRIEPMVVPGVEFEPMLIPHHK
                     GRKRMHLELREGLTRMSMDLKNNLLGSLRMAWKSFTRAPYPALQASETPEETEAEPES
                     TSEKPSDVNTEETSVAVKEEVLPINVGMLNGGQRIDYVLQEKPIESFNEYLFALQSHL
                     CYWESEDTVLLVLKEIYQTQGIFLDQPLQ"
     exon            504..694
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            695..784
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            785..905
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            906..995
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1131
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1340
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1408
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1531
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1627
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1744
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1900
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2003
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2174
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2337
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2445
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..4682
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3284..3289
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /note="hexamer: AATAAA"
     polyA_site      3317
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
     regulatory      4592..4597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /note="hexamer: AGTAAA"
     polyA_site      4613
                     /gene="DDHD2"
                     /gene_synonym="iPLA(1)gamma; iPLA1A; iPLA1gamma; p125B;
                     SAMWD1; SPG54"
                     /note="major polyA site"
ORIGIN      
        1 gcgacacttg ccgcccgtgc cctgcggccc tgcacgcccc acccgcggtc gcgcgccgcg
       61 ctccccgccg cctcaccttt tcgcctggca tccgggcccg ctactcgccc actgggagcg
      121 ccgtggcgcc tggtgttcgg cgcgagcccg gcggggctgc aggttccgcc ctgctccgcc
      181 gcgccccgcc cgggctgggg taaaggccgc ggccgggcca tgcagtgtcc tcctcaggga
      241 gggcaggaga gcctgaggag tggcggggcc gccagagagc gaaatgtcat cagtgcagtc
      301 acaacaggag cagttgtccc agtcagatcc atctccgtca ccaaactcat gtagttcctt
      361 tgagctaata gacatggatg ctggcagctt gtatgaacca gtttctcccc attggtttta
      421 ttgtaagata atagattcta aggagacatg gattcctttc aactctgagg attcacagca
      481 gctggaagag gcatatagct ctggaaaagg ttgtaatggg agagttgttc ctactgatgg
      541 gggcagatat gatgttcatt tgggggagag gatgcggtat gctgtatact gggatgaact
      601 ggcatcggaa gtgagacgat gtacgtggtt ttacaagggg gacaaagaca ataagtatgt
      661 tccctactcg gagagcttca gccaagtttt agaggaaact tacatgcttg ctgtaacttt
      721 ggatgaatgg aaaaagaaac tggaatctcc caacagagaa attattattt tacacaatcc
      781 aaagcttatg gtgcattacc agccagttgc agggtctgat gattggggtt caacacccac
      841 ggagcagggt cgaccaagaa ctgtgaagag aggagttgag aacatctctg ttgacattca
      901 ttgtggagaa cctttacaaa tagatcactt ggtttttgta gtccatggga ttggaccagc
      961 ttgtgatctc cgctttcgaa gcattgtaca gtgtgttaat gattttcgca gtgtttcctt
     1021 gaacttgcta cagacacatt ttaagaaagc ccaagaaaat cagcagattg ggagggtaga
     1081 atttcttcca gtcaactggc acagtccttt gcattctact ggtgtggatg tagatctgca
     1141 gcgaataacc ctgcccagca ttaaccgcct caggcacttc accaatgaca caattctgga
     1201 tgtcttcttc tacaatagtc ccacctactg tcagactatt gtggacacag ttgcttctga
     1261 aatgaaccga atatacacac tttttctaca gaggaaccct gatttcaaag ggggtgtatc
     1321 cattgctggt catagtttag gttcgcttat attgtttgat atcctaacaa atcagaaaga
     1381 ttctttgggg gatattgaca gtgaaaagga ttcgctaaat attgtaatgg atcaaggaga
     1441 tacacctaca ctagaggaag atttgaagaa acttcagctc tctgaattct ttgatatctt
     1501 tgagaaggag aaagtagata aggaagctct ggctttatgt acagaccgag atcttcagga
     1561 aataggaatt cctttaggac caagaaagaa gatattaaac tatttcagca ccagaaaaaa
     1621 ctcaatgggt attaagagac cagccccgca gcctgcttca ggggcaaaca tccccaaaga
     1681 atctgagttc tgcagtagca gtaatactag aaatggtgac tatctggatg ttggcattgg
     1741 gcaggtgtct gtgaaatacc cccggctcat ctataaacca gagatattct ttgcctttgg
     1801 atctcccatt ggaatgttcc ttactgtccg aggactaaaa agaattgatc ccaactacag
     1861 atttccaacg tgcaaaggtt tcttcaatat ttatcaccct tttgatcctg tggcctatag
     1921 gattgaacca atggtggtcc caggagtgga atttgagcca atgctgatcc cacatcataa
     1981 aggcaggaag cggatgcact tagaactgag agagggcttg accaggatga gtatggacct
     2041 taagaacaac ttgctaggtt cgctgcggat ggcctggaag tcttttacca gagctccata
     2101 ccctgcctta caagcttcag aaacaccaga agaaactgaa gcagaacctg aatcaacttc
     2161 agagaagcct agtgatgtta acacagaaga gacctctgtg gcagttaaag aagaagtcct
     2221 gcctatcaat gtggggatgc tgaatggagg ccaacgcatt gactatgtgc tacaggagaa
     2281 gcctattgaa agttttaatg agtatttatt tgctttacaa agccatctat gctactggga
     2341 gtctgaagat acagtattgc tcgtcctcaa agagatctac caaacccagg gtatcttcct
     2401 tgatcagcct ttacagtaaa aatgacccat ctatggctgc ttaatacgga cattgaggga
     2461 tccttcccca gaaaatccac ctgtttgttg ctgcaatttt cctctcctca gctgcgtcat
     2521 ttcctgcatg ttgcctgcca cttactcacc actggggtct ttggaagata atcttcctct
     2581 ttggaaatga atggaaaagc aaaaggccct attactttta accactggct tcatataaac
     2641 acttgccatt tttttctgca tagctggggg tggtttgtgt ctttaattct ttgatgatag
     2701 tttatagttg ccacacttta ttgattagta cttgacaggg tgtaaagcct attttgggtt
     2761 tgatttgttt tgggtggggt agacatgttt ttaaggaact tattgcttat ctttagaaaa
     2821 tgttctagtt tggaaacaga ttcttgagat tcagaaggca ttttggagta cacttatctc
     2881 ttgtttgtgt tgaactgaag gctaagtctc agtggacatg gaaaagactt ttgggtgatt
     2941 tatttttgaa cctgcatttc tttcttatgt gtagtgtatg aagaaagact agaatgtagc
     3001 tttaaaaaag tgttgtttac tctcttagaa ctgacagact tattgccaga aatcactgat
     3061 gttcattgtt tttgcaactg tttgagctgc tgtaagagtc taaagttgac aagttagttc
     3121 atgttaggtg catctttata aagcaaagat gttgtatatc ctaggcctcc cttttatatt
     3181 tgatagaagt tatttgctaa tagcttctat tcttacgttg aaaatagttg taaaagctga
     3241 tgaacctgaa attgtgtagc ctctacaggc tgctgaggtt ctaaataaaa ccttttagtg
     3301 gtgcctttat ggtgaaacag aatttgtcac ctgccatttc tacttgagct aaggtagtat
     3361 tgtgtatcct ctttccttct taggtatcca taatccacaa agcatattta aaaggctctt
     3421 ggcacgggca gcattggttg agcaggtagg tttggctagg gggaaatgtt taacttgttc
     3481 tgaaagaaaa acttatgtct gtagggtcca agaaacagct attccagagt cagtgtcagc
     3541 tgagtctgga acatatgaag tgaggtttac ttctaagaac acaagtgact gcacactaat
     3601 tttgtcaagg catcttttca ctactttgct gtagattttt cttcttcatt ggtcagtttg
     3661 tcattgtctt tgtagttctc tttatgataa tcctttatac ttgctctcag attccacagg
     3721 cctctgttta tagagtggca aaggcaggcg agctgtggtt tattgtttat aaattttttt
     3781 ataaatgtta tggtattcaa agccactgac atttaatatt tactgaagcc attccttaga
     3841 cagcagtggt ctttatccct ttctggaaag aaaaggaaaa tgaagggtaa ttactgtcac
     3901 catggagatt gtagaggtaa ggttggggta taggtcaggc ctggcctttc tttgtcatct
     3961 gcttatagtc tagtgctaag tatgccacta agtttcagat atatggaata ctttattttt
     4021 ttaaaggtat ataaactctg agttattgag aattaagtat tcactgtata ttaaggggaa
     4081 gcttttgcca agttgtggtc ttcaaattta tgtttactct tcctattggc agaataggtg
     4141 ctatttaaga gtaaaccaaa ggataagcag agggagtccc tataaccaaa gatggacagc
     4201 atagccctgg atagccagat aaaccactct ttgtattaag aaatgtttct ttcctagtgg
     4261 tgaggggtgg gtaactgtga aagagcttta tatcttgtct attcatggta ttatagctgt
     4321 atattcccag gatgataagc ttgattgaaa tcctgtattt agtcatatat tatttgcgct
     4381 gcttcatttg tatcatgtgc aatctctaga ccaaccctat ttttaaactc tggtacagca
     4441 tcattttgta catattccca gctgcagaac tagtatcact tatctcagca aaagagattg
     4501 tttgcatgga aagattaata gcactgatta gatttctaat attttgcatt tttgaaatgt
     4561 ttgttttcta cgtgattata tttaaaactt tagtaaatac taacatgaaa ccatattgac
     4621 tagtggccta cttttacctg gactcacccg tctcaaagtc aagaaaatca gtacagtcac
     4681 ga
//
