LOCUS       NM_001160148           12941 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens DDHD domain containing 1 (DDHD1), transcript variant
            3, mRNA.
ACCESSION   NM_001160148
VERSION     NM_001160148.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Matsumoto,N., Nemoto-Sasaki,Y., Oka,S., Arai,S., Wada,I. and
            Yamashita,A.
  TITLE     Phosphorylation of human phospholipase A1 DDHD1 at newly identified
            phosphosites affects its subcellular localization
  JOURNAL   J Biol Chem 297 (1), 100851 (2021)
   PUBMED   34089703
  REMARK    GeneRIF: Phosphorylation of human phospholipase A1 DDHD1 at newly
            identified phosphosites affects its subcellular localization.
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Kitamura,C., Sonoda,H., Anzai,H., Nagai,Y., Abe,S., Yokoyama,Y.,
            Ishii,H., Kishikawa,J., Emoto,S., Murono,K., Sasaki,K., Kawai,K.,
            Nozawa,H. and Ishihara,S.
  TITLE     Expression of Lysophosphatidylinositol Signaling-relevant Molecules
            in Colorectal Cancer
  JOURNAL   Anticancer Res 41 (5), 2349-2355 (2021)
   PUBMED   33952459
  REMARK    GeneRIF: Expression of Lysophosphatidylinositol Signaling-relevant
            Molecules in Colorectal Cancer.
REFERENCE   3  (bases 1 to 12941)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 12941)
  AUTHORS   Dard,R., Meyniel,C., Touitou,V., Stevanin,G., Lamari,F., Durr,A.,
            Ewenczyk,C. and Mochel,F.
  TITLE     Mutations in DDHD1, encoding a phospholipase A1, is a novel cause
            of retinopathy and neurodegeneration with brain iron accumulation
  JOURNAL   Eur J Med Genet 60 (12), 639-642 (2017)
   PUBMED   28818478
  REMARK    GeneRIF: A novel homozygous mutation in DDHD1 was identified in a
            patient with hereditary spastic paraplegia, retinal dystrophy and a
            pattern of neurodegeneration with brain iron accumulation.
REFERENCE   5  (bases 1 to 12941)
  AUTHORS   Tesson,C., Nawara,M., Salih,M.A., Rossignol,R., Zaki,M.S., Al
            Balwi,M., Schule,R., Mignot,C., Obre,E., Bouhouche,A.,
            Santorelli,F.M., Durand,C.M., Oteyza,A.C., El-Hachimi,K.H., Al
            Drees,A., Bouslam,N., Lamari,F., Elmalik,S.A., Kabiraj,M.M.,
            Seidahmed,M.Z., Esteves,T., Gaussen,M., Monin,M.L., Gyapay,G.,
            Lechner,D., Gonzalez,M., Depienne,C., Mochel,F., Lavie,J.,
            Schols,L., Lacombe,D., Yahyaoui,M., Al Abdulkareem,I., Zuchner,S.,
            Yamashita,A., Benomar,A., Goizet,C., Durr,A., Gleeson,J.G.,
            Darios,F., Brice,A. and Stevanin,G.
  TITLE     Alteration of fatty-acid-metabolizing enzymes affects mitochondrial
            form and function in hereditary spastic paraplegia
  JOURNAL   Am J Hum Genet 91 (6), 1051-1064 (2012)
   PUBMED   23176821
REFERENCE   6  (bases 1 to 12941)
  AUTHORS   Ma,J., Dempsey,A.A., Stamatiou,D., Marshall,K.W. and Liew,C.C.
  TITLE     Identifying leukocyte gene expression patterns associated with
            plasma lipid levels in human subjects
  JOURNAL   Atherosclerosis 191 (1), 63-72 (2007)
   PUBMED   16806233
REFERENCE   7  (bases 1 to 12941)
  AUTHORS   Bouslam,N., Benomar,A., Azzedine,H., Bouhouche,A., Namekawa,M.,
            Klebe,S., Charon,C., Durr,A., Ruberg,M., Brice,A., Yahyaoui,M. and
            Stevanin,G.
  TITLE     Mapping of a new form of pure autosomal recessive spastic
            paraplegia (SPG28)
  JOURNAL   Ann Neurol 57 (4), 567-571 (2005)
   PUBMED   15786464
REFERENCE   8  (bases 1 to 12941)
  AUTHORS   Higgs,H.N., Han,M.H., Johnson,G.E. and Glomset,J.A.
  TITLE     Cloning of a phosphatidic acid-preferring phospholipase A1 from
            bovine testis
  JOURNAL   J Biol Chem 273 (10), 5468-5477 (1998)
   PUBMED   9488669
REFERENCE   9  (bases 1 to 12941)
  AUTHORS   Higgs,H.N. and Glomset,J.A.
  TITLE     Identification of a phosphatidic acid-preferring phospholipase A1
            from bovine brain and testis
  JOURNAL   Proc Natl Acad Sci U S A 91 (20), 9574-9578 (1994)
   PUBMED   7937808
REFERENCE   10 (bases 1 to 12941)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA212814.1, BC030703.2,
            AK125556.1, BY796571.2, AL352979.4 and AI871726.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001160148.1.
            
            Summary: This gene is a member of the intracellular phospholipase
            A1 gene family. The protein encoded by this gene preferentially
            hydrolyzes phosphatidic acid. It is a cytosolic protein with some
            mitochondrial localization, and is thought to be involved in the
            regulation of mitochondrial dynamics. Overexpression of this gene
            causes fragmentation of the tubular structures in mitochondria,
            while depletion of the gene results in mitochondrial tubule
            elongation. Deletion of this gene in male mice caused fertility
            defects, resulting from disruption in the organization of the
            mitochondria during spermiogenesis. In humans, mutations in this
            gene have been associated with hereditary spastic paraplegia (HSP),
            also known as Strumpell-Lorrain disease, or, familial spastic
            paraparesis (FSP). This inherited disorder is characterized by
            progressive weakness and spasticity of the legs. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (3) includes an alternate in-frame
            coding exon in the 3' coding region, compared to variant 1. The
            encoded isoform (c) is longer and differs at an amino acid in the
            C-terminal region compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.1483693.1,
                                           SRR14038197.2210983.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000673822.2/ ENSP00000500986.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DA212814.1         6-46
            42-730              BC030703.2         1-689
            731-1982            AK125556.1         1-1252
            1983-2248           BC030703.2         1942-2207
            2249-2523           BY796571.2         311-585
            2524-2933           AK125556.1         1794-2203
            2934-3673           BC030703.2         2809-3548
            3674-12621          AL352979.4         26470-35417
            12622-12941         AI871726.1         24-343              c
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q22.1"
     gene            1..12941
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /note="DDHD domain containing 1"
                     /db_xref="GeneID:80821"
                     /db_xref="HGNC:HGNC:19714"
                     /db_xref="MIM:614603"
     exon            1..1063
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..2928
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /EC_number="3.1.1.32"
                     /EC_number="3.1.1.111"
                     /EC_number="3.1.1.118"
                     /note="isoform c is encoded by transcript variant 3;
                     phospholipase DDHD1; intracellular phospholipase A1 alpha;
                     phosphatidic acid-preferring phospholipase A1-like
                     protein; phosphatidic acid-preferring phospholipase A1
                     homolog; spastic paraplegia 28 (autosomal recessive);
                     phospholipid sn-1 acylhydrolase"
                     /codon_start=1
                     /product="phospholipase DDHD1 isoform c"
                     /protein_id="NP_001153620.1"
                     /db_xref="CCDS:CCDS53895.1"
                     /db_xref="GeneID:80821"
                     /db_xref="HGNC:HGNC:19714"
                     /db_xref="MIM:614603"
                     /translation="MNYPGRGSPRSPEHNGRGGGGGAWELGSDARPAFGGGVCCFEHL
                     PGGDPDDGDVPLALLRGEPGLHLAPGTDDHNHHLALDPCLSDENYDFSSAESGSSLRY
                     YSEGESGGGGSSLSLHPPQQPPLVPTNSGGGGATGGSPGERKRTRLGGPAARHRYEVV
                     TELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGARPQGGDRDGDHVCSP
                     TGPASSSGEDDDEDRACGFCQSTTGHEPEMVELVNIEPVCVRGGLYEVDVTQGECYPV
                     YWNQADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLNCFRGQQMQENFDIEVSKSID
                     GKDAVHSFKLSRNHVDWHSVDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGY
                     VEEATLEDKPSQTTHIVFVVHGIGQKMDQGRIIKNTAMMREAARKIEERHFSNHATHV
                     EFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQ
                     ELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMTGWNPVRLYEQLLQKEEEL
                     PDERWMSYEERHLLDELYITKRRLKEIEERLHGLKASSMTQTPALKFKVENFFCMGSP
                     LAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPV
                     QIHWYNTSNPLPYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESIT
                     NIGKASILGAASIGKGLGGMLFSRFGRSSTTQSSETSKDSMEDEKKPVASPSATTVGT
                     QTLPHSSSGFLDSAYFRLQESFFNLPQLLFPENVMQNKDNALVELDHRIDFELREGLV
                     ESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDDDAKPNLDPI"
     exon            1064..1237
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1366
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1514
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1621
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1728
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1991
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2067
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2217
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2470
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2662
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2746
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..12941
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5711..5716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /note="hexamer: ATTAAA"
     polyA_site      5729
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /note="major polyA site"
     regulatory      12922..12927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /note="hexamer: ATTAAA"
     polyA_site      12941
                     /gene="DDHD1"
                     /gene_synonym="iPLA1alpha; iPLA1I; PA-PLA1; PAPLA1; SPG28"
                     /note="major polyA site"
ORIGIN      
        1 accgcctacg tcgggctgcg tgacaatggg gccggcggca gaacgcagct gcggcggctg
       61 cgggtctcgt gggggcggag cggtcgccgc tgccgccgca gctcgggtcg ggatttgaaa
      121 gattagaaac ttcgggtgga gagggcggcg gcgttgaatg tgtggcggaa gcgctggggg
      181 tcacggctcc gcgcgccgcc ggacagccgg cggcgtctcc acagcatgaa ttacccgggc
      241 cgcgggtccc cacggagccc cgagcataac ggccgaggcg gcggcggcgg cgcctgggag
      301 ctgggctcag acgcgaggcc agcgttcggc ggcggcgtct gctgcttcga gcacctgccc
      361 ggcggggacc cggacgacgg cgacgtgccc ctggccctgc tgcgcgggga acccgggctg
      421 catttggcgc cgggcaccga cgaccacaac caccacctcg cgctggaccc ctgcctcagt
      481 gacgagaact atgacttcag ctccgccgag tcgggctcct cgctgcgcta ctacagcgag
      541 ggtgagagcg gcggcggcgg cagctccttg tcgctgcacc cgccgcagca gcctccgctg
      601 gtcccgacga actcgggggg cggcggcgcg acaggagggt cccccgggga aaggaaacgt
      661 acccggcttg gcggcccggc ggcccggcac cgctatgagg tagtgacgga gctgggcccg
      721 gaggaggtac gctggttcta caaggaggac aagaagacct ggaagccctt catcggctac
      781 gactcgctcc gcatcgagct cgccttccgg accctgctgc agaccacggg tgcccggccc
      841 cagggcgggg accgggacgg cgaccatgtg tgctccccca cgggcccagc ctccagttcc
      901 ggagaagatg acgatgagga ccgcgcctgc ggcttctgcc agagtacgac ggggcacgag
      961 ccggagatgg tggagcttgt gaacatcgag cctgtgtgcg tgcggggcgg cctctacgag
     1021 gtggatgtga cccaaggaga gtgctacccg gtgtactgga accaggctga taaaatacca
     1081 gtaatgcgtg gacagtggtt tattgacggc acttggcagc ctctagaaga ggaagaaagt
     1141 aatttaattg agcaagaaca tctcaattgt tttaggggcc agcagatgca ggaaaatttc
     1201 gatattgaag tgtcaaaatc catagatgga aaagatgctg ttcatagttt caagttgagt
     1261 cgaaaccatg tggactggca cagtgtggat gaagtatatc tttatagtga tgcaacaaca
     1321 tctaaaattg caagaacagt tacccaaaaa ctgggatttt ctaaagcatc aagtagtggt
     1381 accagacttc atagaggtta tgtagaagaa gccacattag aagacaagcc atcacagact
     1441 acccatattg tatttgttgt gcatggcatt gggcagaaaa tggaccaagg aagaattatc
     1501 aaaaatacag ctatgatgag agaagctgca agaaaaatag aagaaaggca tttttccaac
     1561 catgcaacac atgttgaatt tctgcctgtt gagtggcggt caaaacttac tcttgatgga
     1621 gacactgttg attccattac tcctgacaaa gtacgaggtt taagggatat gctgaacagc
     1681 agtgcaatgg acataatgta ttatactagt ccactttata gagatgaact agttaaaggc
     1741 cttcagcaag agctgaatcg attgtattcc cttttctgtt ctcggaatcc agactttgaa
     1801 gaaaaagggg gtaaagtctc aatagtatca cattccttgg gatgtgtaat tacttatgac
     1861 ataatgactg gctggaatcc agttcggctg tatgaacagt tgctgcaaaa ggaagaagag
     1921 ttgcctgatg aacgatggat gagctatgaa gaacgacatc ttcttgatga actctatata
     1981 actaaacgac ggctgaagga aatagaagaa cggcttcacg gattgaaagc atcatctatg
     2041 acacaaacac ctgccttaaa atttaaggtt gagaatttct tctgtatggg atccccatta
     2101 gcagttttct tggcgttgcg tggcatccgc ccaggaaata ctggaagtca agaccatatt
     2161 ttgcctagag agatttgtaa ccggttacta aatatttttc atcctacaga tccagtggct
     2221 tatagattag aaccattaat actgaaacac tacagcaaca tttcacctgt ccagatccac
     2281 tggtacaata cttcaaatcc tttaccttat gaacatatga agccaagctt tctcaaccca
     2341 gctaaagaac ctacctcagt ttcagagaat gaaggcattt caaccatacc aagccctgtg
     2401 acctcaccag ttttgtcccg ccgacactat ggagaatcta taacaaatat aggcaaagca
     2461 agcatattag gggctgctag cattggaaag ggacttggag gaatgttgtt ctcaagattt
     2521 ggacgttcat ctacaacaca gtcatctgaa acatcaaaag actcaatgga agatgagaag
     2581 aagccagttg cctcaccttc tgctaccacc gtagggacac agacccttcc acatagcagt
     2641 tctggcttcc tcgattctgc atatttcaga cttcaagaat cgttctttaa tctcccacaa
     2701 cttctttttc cggaaaatgt aatgcagaat aaagataatg ccctcgtgga gttggatcac
     2761 aggattgatt ttgaactcag agaaggcctt gtggagagcc gctattggtc agctgtcacg
     2821 tcgcatactg cctattggtc atccttggat gttgcccttt ttcttttaac cttcatgtat
     2881 aaacatgagc acgatgatga tgcaaaaccc aatttagatc caatctgaac tctcttgaag
     2941 gacatgaatg gcctaaaact gatttttttt ttttccgtta aaatgtgtgt gtcaagatac
     3001 ggagatttca gggttaaagt atatttcagt tttctttagg gcaacatata tttgaattta
     3061 aaagcacttt atttaaaaaa aaaagaagtt ttcagttctg aagaagtcat ttacagtttg
     3121 catcatttta attatgagtc tgacaaaacc ttctccagag aatcaagcaa gacctggatg
     3181 tgaagaaggt ttgggtaaac tgcatgtaaa ggctacaaat cacaatctga ttcctcccaa
     3241 atataaaggc atatggaaca taatgtatta accaaagtat gttataaatc aaaaatggtc
     3301 aaggttcagc atattctata tgaagatcac aaggtggtat cgttttagat ttctatgaag
     3361 gctttcattt gtacatccct ttgaaaaaat ataacagatt taaaatgttt tgaatttaac
     3421 ttgtttagaa aaactaatgc ttaaaacaat atttgaacta ctgtatttat aatttattac
     3481 ctctaattgc tttatttcag tgtatgagac attactgttt taatgtttgc tttgaacata
     3541 atttaagaac cagatttatt ttctatagtg ataaaccctt ttttctcaga actccatctt
     3601 tgtactcttc agatgaatat atagacactg tggcatacat tttttttcat taaaaactta
     3661 tggcttcata caacactagt tcaatttttt aaataaactt tttattatgt tacatgtact
     3721 tcagagaaag ctaaagtttc tctaaacttg acacggagta ctccataatg ggtacatttc
     3781 attagcttta ttagaaaact agtaaatgct tttgaatagg tagtatgaca gctattttaa
     3841 atgtataaac atttgtgtgc caaacaacat atatgaaggg atgtgatgcc actaactagt
     3901 gcacattgtt tctcaatgtg ccattgagac acaaggcaca gtaagtcagg attctgaggc
     3961 attggaaggg ttttcttcag ctgtaattgt ttccacagtg cttttcatct gaggggctca
     4021 aagtttaaga gtgcattcaa taacccatgt actagcttct tgaggtaagt aaataaatct
     4081 tgttctcatg ttacatgtta atacaccaag gcaccaaaac atttaaagtg ccatgaatta
     4141 gattacatca gaaatcagaa aagcttggat tgagctagtc accgtggttc acgcctgtaa
     4201 tcccaacact tcgggaggcc aagatgggag gatctcttga gcccaggatt tcaagaccag
     4261 cttgggcaac atagtgagac cctcttctct gcaaaaactg acaaaaatta gccacttgta
     4321 gtggtgtaca cctgtagtcc cagctactca agaggctgag gtgggaggat cacccgagcc
     4381 cagaagttca aagctgcagt gagctctgat cacaccaatg cactccagcc tgggtgacag
     4441 agccagaccc tgtctctaaa aaacaaaaaa gaaaagcttg ggttgaagcc aggccatatt
     4501 cttttatatg ctgttcaatg acttgattca catgtgacct tttagtagtt tgcaaacaat
     4561 aataacagaa gttgcaattc ataattcaga gaaatgacca tgaagccggt gtttccctgc
     4621 ttgatttcta tgttcttgga ttctcacaca aaagaggagt tacctagagg ggatcagtag
     4681 ccatccctgt tgcacacaac cctcacttct gagtggtttc cactaggttc cacttaaggt
     4741 gctgtctttt cttaacttgc aaggaattgc ttagcttccc aataatcatt gtcaaactgg
     4801 gagcaaagtt ggtcagctgt atctctcacc tcctcctttc ttcataagat agtagaatct
     4861 gtggcttact tgtaggtttt acttagtgct tcagtactgt tggctgtttt ggccttttgc
     4921 tgaatttgca caacaacata aagcacttct ttttaatgtt taaaaaaaat tccaaggctc
     4981 catgaccaag tatgtgactc actaagaaac ccttgctata aaaggctttt gatgaaatct
     5041 tagcaaagct aaattatctc taacttagag attcctatga actcaggtca ttttcatagc
     5101 ctgtttattt tgtaggtcta atttagttct caggaaaatt aaactcatgc tatatgactg
     5161 tatcgttaaa ggtacttgag cgtatgttgc tgtaggtggt atgacactca aatgcataag
     5221 aacttgaagg gatttattct taaccactag aatatgaaga gcccttttta cctgagaaga
     5281 gtgaaattat gtcagacctt ttaatttgat atcactgagt aaaaggtatg ttcctatagc
     5341 cataggaata tgtctgcttc ctttttttca tgttgagaaa acagtcaaat ttaaccattt
     5401 ggagtttatt tgaattcttg gaaagagcaa tattcaaaac cttttcaaaa ataagatatt
     5461 ctggactact ggatggatgt tttgttttca gttcattttt catcagtaga gatggtgatt
     5521 tcggttttgt agtatccttg tttctatgtc tgtgcatgtt aacattggat gtatttgtat
     5581 atacttaatt aatatacaga ctgtgtgtca ttctggcttg attagaagct aagtcagtta
     5641 ctgagtaaca ttttgcaact ttattccagc aagtactaaa tcggccaaaa aaagttttgt
     5701 ttttgatacc attaaaattt tattctctat tttgtgtaca tgctatgatg attctcccaa
     5761 gtactgatta gatcactgaa ctttagttag cttcccaaga attattaacc gttagaataa
     5821 gacttagatt aaatcttgct ttatttttac tttaaaatta gtcttgctaa ggccttatgt
     5881 tagttcatac tttcttttca acagtaatct tgtttataat ccagtgtttc tgtgttttag
     5941 gacataattt aaaaatatac tggattttgg ccaggtgtgg cagctcatgc ctgtaatccc
     6001 agcactttgg gaggccaagc agggaagatt acttgagccc aagagttgaa aaccaacctg
     6061 gacaacatgg tgaaacccag actctacaaa aaatgcaaag attagctggg tgtgttggca
     6121 gacgcctgtg gtcccagcta ctcaggaggc tgaggtcggt aggactgcat gattccggga
     6181 ggtcaaggct tcagtgagcc aagattgcac cactgcactc caccttgggt gacagagctg
     6241 gacactgtat ccaaaaaaaa aaagtatgta tttacacaca cacacacaca cacacacaca
     6301 cactggatgt tctaataact gctcttttgt atgtgaagca agccttagga atcttacgtt
     6361 ttacttcttt ctgttaaacg gcatctgtta tcttttgaag tactcacact gaacatgctt
     6421 catgctgaat actcacgcta aaggcctcag acatgtaaac attcatcctt agtctctttt
     6481 ttctctgttt tctcattggg agctgaagca tcctagtctg cagaaaattt aacacaccat
     6541 attccagctt tgcatcacct agacagtaga caaaaggaaa agaaattgtc agtaccgtat
     6601 cacagctagt gcctctgttc attccagtga tagtacatga gccccttcca gtggagtcag
     6661 atcaagccaa aagattttcc tgtaaagtct gtagtagaag acaaatttct ttttgatcca
     6721 ttagattggt atttctatct tctcagctaa ttaatgagta aggcatgtgg acaatcgaga
     6781 aaactaattt gagtgtatgt attaaaattg ctcttctaaa aacacgtaaa atattaaaga
     6841 tcaaggcaga gcaaaaaaga acgtagcaat catatactag aaaaagaaac taagttttgc
     6901 taaactatgt ttgggttagt tgacctctgt agctattcta aagttttcta tcccttatct
     6961 gtattccgct gtcattcctg tgcctcaagc ctctgccagg aatctgatgg tactctggct
     7021 gaacttaaaa cataagaatc tcaaaggtct catttagtaa atgttagtgc taagttggtg
     7081 acagcctggg ttatatgctt tctctttcag tcttcagtag atgcttgtga tagttttcaa
     7141 agaactgtaa aatttccggg tctgtacaga tttttagaga tcatttaatc caaccctgtt
     7201 acagtatcat gtcagcattt aaatagcagt aaaggtcatg aaaagtgaag tgaaaggcag
     7261 caaactccat gaaaaactaa atacagtgac aactgaggga agtggtaaag taaaaaaata
     7321 ttaatgatgg aattttaaag ctggaagggg catagagacc attcttttct caataatatt
     7381 ctgcaaatat taacaggtgt catgtcagaa actaagttta ggaattgaca aattcaaaaa
     7441 tctcccttac agcagggctt tgagagcctt tgctacacta aatgctctgt gaatctatct
     7501 ggtgctgcgt gggggcctcc aaatttattt aaccatattt tttaatttgg catttaccta
     7561 gagaaatgct atcacattta attccttcgt tttacagttt cacctcctca tgtgattcct
     7621 tcattttgta agtgaccgat tccaccctat acaatcacat ggccaatgaa tgacagtcag
     7681 aattagaacc tagtcccctg attcccaacc atgtcttata ccagactgtt tcccctaatc
     7741 gataggaaac tactcctaaa gggcagaaac tgcctctctt tgtttttgtc ccccaacctc
     7801 ccctccacac atacacctct gtaatctgat gctttaagct cagattaaag catcagcaag
     7861 ctttaatctg acacatggcc gtaggcttga gttagcagtt ggtcccttct ttcttccaga
     7921 ctctcataag agtaccctat caggtctgga cagacttccg tttatgcctc agattcaaaa
     7981 tacccaggaa aaagaagaag aaatacaaaa caacaaaaag aaaatcaaga aaaaaataag
     8041 atatccagga aatattttgt tagttttcta ttctggtgac aaatgcaaac ttaaggaaac
     8101 aagttgcaca gtgttaacta atgtttaaca ttcctttcca tagtgtttat gtgggccttt
     8161 tatcaattca tgacaaatag gtcacctcca aaactgttcc aacataggtt aactcagaaa
     8221 tttaaaattt aagtactatc ctttttggag actatgagaa gagtttttac tattgtgttt
     8281 tgttttgttt tttggtgtgt gtgtgtatgt atgtgcattc cccaaaaata gtccattggc
     8341 agattgttag ggtgaacttt tcctctgcct ttgtgtttgt ataagggcgg gtgcatgctt
     8401 agtattgtct gctaccttat ggtttttcat ttatatgtaa catggtacaa cttgcttcct
     8461 ccccacccaa cagttgtcag gatatgttgg ggaatattct ttaggaagat tacatctggt
     8521 cttctgttgg agtaagatga aaaacaagta tgttaacact tgacctttac tgctttctcc
     8581 tggaagacat tgaatatcta ggagcgtcac tcataccttc ccttgactgt acccacaccc
     8641 actgcggtcc ctgcttgatc tctcaaactt tttgtttccc tttgagcttc atgacaaaaa
     8701 attataacca ttttctggtt ctaaggctgc cataatccca actttgccag taagaagatg
     8761 gagaattttt gctcataggg acatttctca gaagcagctg gcaccataac tctcctgaga
     8821 cccttgtttc ctttctttca gctcctatga cttcttatgt tcactctctc taacactact
     8881 aaatgttgtc aatgactgca atcacaactc tttgaaggca ggctttttct tttcctttgt
     8941 tacctcttat tttcaatagc caaagcaggt tagttttctc agaatcttca gttttactga
     9001 agcatgcatt atttctgttt tctctctgca gtgatcagtt tggatgacag aaactttaaa
     9061 cttttattct ggcattgtcc agttctgatt ttctcagctg gaactggtgc atgcacattt
     9121 aataatttca aagaagtgag aggtaaatgt tttctcctgc attatactgt gtttctctag
     9181 ctgaattgat ccgtttgtta tcttgctcac ccgaaacaag tagttgtgcc ctacagtgag
     9241 caacctcaga ctaggctgtt ctaagcattc tggagaaaat aatcctcatc atacagtttg
     9301 tggtaactct tccaaaaagt ttatgaaaat tgaaagatct ccctatgcat cagctgtggc
     9361 tgccacattc cgttctacta ggaaggctag tggccacaac tccctctaga acatcagagg
     9421 ataaagtaca cttatatatt agaaaagaca ggcatattct gattatggtt cattcacaat
     9481 ttctcagtcc agggttcagt cagtgaccat agctctgagg tcttcaggct aactccaaga
     9541 acccgtgcca gatagaaacc tactcctact acctttccac tcaccttccc attcccccac
     9601 ctttgctacc aaagtgactt taaaagtcga gttttaggtg tttgtttttt taacagtcta
     9661 ggaatttcag tgcaattccg tgaggtggtg ctgaccttag atgagaaata cgtggccagg
     9721 ctataaggac tacatgtaga attgagatgg gacagtgtac gtatggactg tgaggggaaa
     9781 gaaaaggtaa atgtgtgaaa ggaaagagat tggtgcatgg tcatgacagt ctgacagctt
     9841 agacatttca gaggcattgt ttatgagaaa ggggataggg acacataggt ctgatgacaa
     9901 ccaaagccct ttgatgatgc catctgtcac tcaaggctcc ccacagcctg cccaacctga
     9961 ctctcctgcc tgcttctcca ctgcctacct tcaacaatca aactatattt ttgttacagc
    10021 aaactacatt ccatttgcct ttaaatgctt gcattttagt tattgtactg gctacctgtt
    10081 tttgtctgcc cagcatcctg tttcccctcc ttttggatcc tctcctagcc aattccatgt
    10141 cttgaatcct ttcctgctcc ttgttaaaat tattttctgc tttgtgtgag tccctgtaag
    10201 caccagcagc tcaatcagca ctgtctgtac catggtcaag agatgagtac atgactcagg
    10261 tcagacctta tttcccaccc cataagccac aatgattaga caagaaatag gcacagaacc
    10321 ctaactagat agggcagaag ccttccatag gattttattt gctggccctg aaatgcaggt
    10381 agccttctat ggctgttaaa cacaatccag tggcacatgg ggtgatatga ggatggagcc
    10441 atccatggca aaactccacc ctttcctgtg acatggatta tgtgcatctg ccatgaaaag
    10501 gaagcataca caagaaatga gccaagagtt cctggagaac tgaagcaagt atcacctcca
    10561 gatcagttgt acttttattt gcttacacta ttctgaggtg ggtctctgtc tcttggatcc
    10621 aaaagagttc aaattaataa tcatttgaca aaaaattacc tccacattcc taatgaagtt
    10681 gtctttgaag ataatgtctt gtgatcccct ggttgaagtt aattactgct tgtgagcccc
    10741 cattaacaac gtgttccttt ccccattgct tgcttgtatc agctttgctg aagatctatt
    10801 ggctgtaggt gtgcagcttt atttctgtgt tctctattgt gttccattgg tctacgtgtc
    10861 tgttattgta ccagtgccgt gctgttttgg ttactgtggt cttatagttt gaagccacat
    10921 ctgtgtgatg ctgctggctt tgttctttct gctttgggtt gctttggcta ttcaggctct
    10981 ttctttggtt ccatctgaat tttagaatag ttgttttcta attctgtgaa aatgttccaa
    11041 cctctgtgaa aaatgaggtt ggtagtttga taggacagca ttgattctgt aaattgcttt
    11101 gggcagtatg gccattttta aactatattg attcttccaa tccatgaaca tggaatgttt
    11161 ttccattttt tggtttcatc cctgatttct ttctgccatg ttttgtagtt ctccttgtag
    11221 agatctttca cctccttggt taggtgtatt tttaagtatt tcagtttttt tatggctatt
    11281 gtaaatggta ttgggttctt gatttgctct cagcttgaac gttattggtg tatagaaatg
    11341 ctcctaattt ttgtgcattg attttgtatc ctgaaacttg actaaagttg tttatcagtc
    11401 taggagcttt tggcagagtc ttcggggttt tctaggtata aaatcatatc agcgaagaga
    11461 gctagtttga tttcttttcc cagttggatg ccttttattt atttctcttg cctgattgct
    11521 ctaagttgaa tgggagcgat gagactgggc atcctcttct tattccagtt ctcagaagga
    11581 atagttccag cttttgctca tccagtatga tgtctgtggg ttggttgtag atggctctta
    11641 ttattttgag atatgttcct ttgataccta gtctgttgag gggttttatc atgagggatg
    11701 ttggatttta tccgtattca ataaatggtg ttgggataac tggctagccc tatgcagaag
    11761 aatgaaactg gaccccccac ctttcaccgt atatgaaaat taactcaaga tggattaaag
    11821 atttaaatgt aagacctcaa actgtaaaaa tcctagaaga aaacctagga aataccctta
    11881 tcaacatcag ccttggcaaa gaacttttgg ctaagtcccc aaaggcaatt gcaacaaaac
    11941 agaaattggc aagtggggac ctaattaaag cactctgcac agcaaaagac actatcaaca
    12001 gagtaaacag acaacataca gaattggaga aaatatttgc aaactagaca tccaacaaag
    12061 atctgacatc cagaatcaat aaggaactta acaagcaaaa aacaacccca ttaaaaaatg
    12121 ggtgaaggac atgaacagac acttctcaaa agaagacata caagcaatca acaaacgtga
    12181 aaaaatgctc atcactaatc atcagagaaa tgcaaatcaa aaccacaata agataccatc
    12241 tcacaccagt tagaatggct attgtaaaga agtctaataa catgccagta aggtttcaga
    12301 gaaaagagaa catttataca ctgttgtggg aatgtaaatt agttcagtca ctgtggaaag
    12361 cagtttggag atttctcaaa taacttaaaa cagatctacc attcaaccca gcatatgggt
    12421 ttatttccca aaaggaaata aatccttcta ccaaaaacac atatggtcat cacagtgcta
    12481 ttcacagtag caaagacaga tcaacgtggc tgcccatcaa cagtggactg gataaagaaa
    12541 atgtggtaca tataaatcat ggagtattag gcagccataa aaagaacaaa atcatgtcct
    12601 ttgcagccag ccacatggat acagctggaa gccataatct aagcaaatta agaacagaaa
    12661 accaagtact gcatgttcta acaaatggga gctaaatatt gagtacctca ggatgcaaag
    12721 gtgggaacaa cagacactgc agactggaac actgtgggtg aggagggcag aaggataggt
    12781 tgaaaaacta cctattgggt actatgctca ctacctgggt gatgggatct gtacctcaaa
    12841 cctcagcatc acacaatata cccttgtaac aaacttgcac atttacccac tgtttctaaa
    12901 tgaaaagttg aaatattttt tattaaaaaa cacaaaagca a
//
