LOCUS       NM_032299              12675 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens defective in cullin neddylation 1 domain containing 5
            (DCUN1D5), transcript variant 1, mRNA.
ACCESSION   NM_032299
VERSION     NM_032299.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Lin,Y., Li,Y., Chen,X., Jin,X., Jiang,M., Xiao,H., Chen,L.,
            Chen,M., Zhang,W., Chen,H., Nie,Q., Guo,R., Guo,W., Fu,F. and
            Wang,C.
  TITLE     YY1 mediated DCUN1D5 transcriptional activation promotes
            triple-negative breast cancer progression by targeting FN1/PI3K/AKT
            pathway
  JOURNAL   Biol Direct 19 (1), 42 (2024)
   PUBMED   38831379
  REMARK    GeneRIF: YY1 mediated DCUN1D5 transcriptional activation promotes
            triple-negative breast cancer progression by targeting FN1/PI3K/AKT
            pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 12675)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 12675)
  AUTHORS   Fang,Y., Yu,B., Liao,G. and Liu,H.M.
  TITLE     Targeting the DCN1-UBC12 protein-protein interaction: novel
            approaches and future directions
  JOURNAL   Future Med Chem 11 (22), 2869-2871 (2019)
   PUBMED   31668094
  REMARK    GeneRIF: All reported DCN1 inhibitors are able to induce remarkable
            accumulation of cullin 3 and its substrate NRF2, indicating
            therapeutic potential of DCN1 inhibitors for human diseases that
            may benefit from upregulation of cullin 3 and NRF2
REFERENCE   5  (bases 1 to 12675)
  AUTHORS   Thomas,Y., Scott,D.C., Kristariyanto,Y.A., Rinehart,J., Clark,K.,
            Cohen,P. and Kurz,T.
  TITLE     The NEDD8 E3 ligase DCNL5 is phosphorylated by IKK alpha during
            Toll-like receptor activation
  JOURNAL   PLoS One 13 (6), e0199197 (2018)
   PUBMED   29958295
  REMARK    GeneRIF: evidence that DCNL5 may be involved in innate immunity, as
            it is a direct substrate of the kinase IKKalpha during immune
            signalling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12675)
  AUTHORS   Monda,J.K., Scott,D.C., Miller,D.J., Lydeard,J., King,D.,
            Harper,J.W., Bennett,E.J. and Schulman,B.A.
  TITLE     Structural conservation of distinctive N-terminal
            acetylation-dependent interactions across a family of mammalian
            NEDD8 ligation enzymes
  JOURNAL   Structure 21 (1), 42-53 (2013)
   PUBMED   23201271
REFERENCE   7  (bases 1 to 12675)
  AUTHORS   Guo,W., Li,G.J., Xu,H.B., Xie,J.S., Shi,T.P., Zhang,S.Z., Chen,X.H.
            and Huang,Z.G.
  TITLE     In vitro biological characterization of DCUN1D5 in DNA damage
            response
  JOURNAL   Asian Pac J Cancer Prev 13 (8), 4157-4162 (2012)
   PUBMED   23098533
  REMARK    GeneRIF: Overexpression of DCUN1D5 is associated with laryngeal
            squamous cell carcinoma.
REFERENCE   8  (bases 1 to 12675)
  AUTHORS   Meyer-Schaller,N., Chou,Y.C., Sumara,I., Martin,D.D., Kurz,T.,
            Katheder,N., Hofmann,K., Berthiaume,L.G., Sicheri,F. and Peter,M.
  TITLE     The human Dcn1-like protein DCNL3 promotes Cul3 neddylation at
            membranes
  JOURNAL   Proc Natl Acad Sci U S A 106 (30), 12365-12370 (2009)
   PUBMED   19617556
REFERENCE   9  (bases 1 to 12675)
  AUTHORS   Tegha-Dunghu,J., Neumann,B., Reber,S., Krause,R., Erfle,H.,
            Walter,T., Held,M., Rogers,P., Hupfeld,K., Ruppert,T., Ellenberg,J.
            and Gruss,O.J.
  TITLE     EML3 is a nuclear microtubule-binding protein required for the
            correct alignment of chromosomes in metaphase
  JOURNAL   J Cell Sci 121 (Pt 10), 1718-1726 (2008)
   PUBMED   18445686
REFERENCE   10 (bases 1 to 12675)
  AUTHORS   Kurz,T., Ozlu,N., Rudolf,F., O'Rourke,S.M., Luke,B., Hofmann,K.,
            Hyman,A.A., Bowerman,B. and Peter,M.
  TITLE     The conserved protein DCN-1/Dcn1p is required for cullin
            neddylation in C. elegans and S. cerevisiae
  JOURNAL   Nature 435 (7046), 1257-1261 (2005)
   PUBMED   15988528
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB039638.1, BC004169.2 and AP001486.5.
            
            On Nov 22, 2018 this sequence version replaced NM_032299.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.183467.1,
                                           SRR1803614.105304.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260247.10/ ENSP00000260247.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DB039638.1         56-75
            21-1269             BC004169.2         1-1249
            1270-12675          AP001486.5         98479-109884        c
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.3"
     gene            1..12675
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="defective in cullin neddylation 1 domain containing
                     5"
                     /db_xref="GeneID:84259"
                     /db_xref="HGNC:HGNC:28409"
                     /db_xref="MIM:616522"
     exon            1..374
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..1002
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="isoform a is encoded by transcript variant 1;
                     DCN1-like protein 5; DCUN1 domain-containing protein 5;
                     defective in cullin neddylation protein 1-like protein 5;
                     DCN1, defective in cullin neddylation 1, domain containing
                     5; squamous cell carcinoma-related oncogene 5"
                     /codon_start=1
                     /product="DCN1-like protein 5 isoform a"
                     /protein_id="NP_115675.1"
                     /db_xref="CCDS:CCDS8325.1"
                     /db_xref="GeneID:84259"
                     /db_xref="HGNC:HGNC:28409"
                     /db_xref="MIM:616522"
                     /translation="MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEE
                     HFSSKKCLAWFYEYAGPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFT
                     KEEWLKGMTSLQCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFARDKDQRSLDID
                     TAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHADLSNYDEDG
                     AWPVLLDEFVEWQKVRQTS"
     exon            375..466
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            467..537
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            538..629
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            630..738
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            739..843
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            844..946
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     exon            947..12675
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1249..1254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="hexamer: AATAAA"
     polyA_site      1287
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="major polyA site"
     regulatory      1509..1514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="hexamer: AATAAA"
     polyA_site      1535
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
     regulatory      11759..11764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="hexamer: AATAAA"
     polyA_site      11783
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
     regulatory      12651..12656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
                     /note="hexamer: AATAAA"
     polyA_site      12675
                     /gene="DCUN1D5"
                     /gene_synonym="DCNL5; SCCRO5"
ORIGIN      
        1 gcttgctgtg actggtggag ctgccgcgct gtccgcgtta tctcctcccg gtgagaacga
       61 accgcagtgt ccaccggcga ggagccagcc ctgtcccggt cagagaaaga cgacgaggat
      121 acctgggagc gggcggcggc cgggctgggc cgcgccggtg cgggctggcg actctgctcc
      181 tccgcttgct gctgtctctg ggaactgggt gccagcgctg aggggcttcc agcggacagg
      241 gacccccttc cccggctccc ctgcccaccc tgccggggag ggcggaagat gccggtgaag
      301 aagaagagaa aatcccctgg ggtggcagca gcagtagcgg aagacggagg cctcaaaaag
      361 tgtaaaatct ccagctattg cagatcccaa ccccctgcta gactaataag tggagaggaa
      421 catttttcaa gcaagaagtg cctggcttgg ttttatgaat atgcaggtcc tgatgaagtt
      481 gtagggccag aaggaatgga aaaattttgt gaagacattg gtgttgaacc tgaaaatatt
      541 attatgttag ttttagcgtg gaaattggag gctgaaagca tgggattttt taccaaggaa
      601 gaatggttaa agggaatgac ttcattacag tgtgactgca cagaaaagtt acaaaacaaa
      661 tttgactttt tgcgctcaca gttgaatgat atttcgtcat ttaagaatat ctacagatat
      721 gcctttgatt ttgcaaggga taaagatcag agaagccttg atattgatac tgctaaatct
      781 atgttagctc ttctgcttgg gaggacatgg ccactgtttt cagtatttta ccagtacctg
      841 gagcaatcaa agtatcgtgt tatgaacaaa gatcaatggt acaatgtatt agaattcagc
      901 agaacagtcc atgctgatct tagtaactat gatgaagatg gtgcttggcc tgttcttctt
      961 gatgaatttg ttgagtggca aaaagtccgt cagacatcat agcaagaact atgtgaagaa
     1021 aatgcaaacc tttcaattcc cacgtgtata caagctaatg tgatgagggg gaaaaaaatc
     1081 caacgggtgc attttcattc atatgaaaga cttctcatag tacttttttt tccttttttt
     1141 aaaggaggtt tttcttgtta catgtgatgg gcattgagcc acacctcttc ttagactgaa
     1201 tattgaagtt tttgttttga gttatgttta taacatttat ttcagaacaa taaagattca
     1261 gatttgtgac aaaggcctta aagtgaagat gtcccttgag tgtcagagtg gtatttattt
     1321 ttgtaaattt gaattttttg atttttgagg ttccaattca ccgtgaattt acagaatttt
     1381 caagatttta aacagtctta atatttggca acatagtctg ctgatggtat ggctcattat
     1441 agtcatctct gcacaatttg gaagactgtt ggccttgact tctgggataa tgatttgctg
     1501 atgcagctaa taaacatttc taggtattgg aaaaatgctc ctacttttag ctctgggttt
     1561 ctataaaagc tgccaaggtc ttatgcagat gtcttatctc agagagctta gaattggaaa
     1621 gattatgtcc agttgttttg ttttctaaaa aagttgtatt attaagttgt atcatcatta
     1681 ttattgttga taatagcata tttatatttt taactgtcat tatataggga attttgttta
     1741 aagcactttt tttttttttt tttgccttaa gagaacgtta gctgtgatat ggatttaatt
     1801 aagatacaca taggaaaaga agacaataga ataaggaatc atgagagaac tctgggtata
     1861 attaattgtg cacaactcaa tagagccatt tacgttggta gtattgcagc ttgctaatta
     1921 tcacaatttt ctgcctgttg gtaatcaagt gtcttgagga aagggaaggc tttcctaatt
     1981 catgtaaaag gcacttttgt actagcagca ggcctgtttg tacattcctt ttcaaaccat
     2041 ccagtatccc agtgatatga aaactttgtt catattcata gcccagtcac aacttcagaa
     2101 acatttctag tgtcattgtt tgagtagaga gaagtaaaga ggttattttt ccataataag
     2161 ggagtgtttc tattaggatt taaggtaaag gttgattttt cagtggtgaa tttgtttaaa
     2221 gaatgatttc tccatattga ttacctaaga gaatgacatt ttaaagtgtt ttattgtttg
     2281 ctttttagtt aatcagctgg gaaaaaaatg attgccactg ttttacttgc cttcttaata
     2341 agattacttt gtacagtttt ctgaaacacc cataatagtg ggagacagtt ttcctaaatg
     2401 tcttatagaa aaggataata cagaatgctc ctcttaagta ttagaatcac tgagttatga
     2461 actgagcata ccagtggtgt taggatttaa tattatgaat gatgttttaa tatcaaattt
     2521 attataagga aagcatttag atttgtagac ctttaaattt ataatttagt tgactcttca
     2581 aggcacatct gaactgaaga acatatccac ttttgttaaa ttccaaaaca tataagcaaa
     2641 atcttataaa ttgtccattt gtaacctgaa ctaatggtaa tatctttttc atcttcagta
     2701 tttgagtaaa atagggaaca cttaaagctt atagatgaca tgttttttaa aaggtatatt
     2761 tatgaaagca caggcatcct tggtatacgc aggagattga taccaggacc cttgtgtata
     2821 cccaaatctg tgcatactta agtcctacag tcatccctgt ggaacctgcc tgtataaaaa
     2881 gtcagccctc cccgtgtatg taggtgtcgc atcccgggaa tactgtattt tcaatccatg
     2941 tttggtttaa aaaaatctgc gtataagcag acccacacag ttcaaactca tgctgttcaa
     3001 gggtcaacag tatttacttt gcatacttat ccaaagatac ctttaagact ttttgagtct
     3061 gaaaatgaaa tttactttat tttttccctt aaagtcaact gtcctatcct accctagcca
     3121 tactttggag tccagtacca ctgaggccac gcttgcttta ctctattgct ccatctattt
     3181 ctcttacctt cttaaagtta taacatttgc ctactttgaa ggcaaattta ctctgcctaa
     3241 ttcatcagtc agggtgtggg ctgtgtccct tcctgtcttt cctttgttaa ttttgaatag
     3301 catttaattt actgggaaat ttatgatcaa aaatgttaag tgatataaac aatacaatat
     3361 taaggaccct gcagtataaa aaagtctaac tgttgtggtt ccttttttga ctagttagct
     3421 ttaagtgcct tcccacgaac cctgttgtaa aattttcaga actacttccc tttgtacagg
     3481 atgaaatacc gtatggtgct ttataacatg gagttgagtt gtttctataa gaatattata
     3541 caaatgtaat actactggga gaaagtagag ccagatgttg attggacaga ttgcaacccc
     3601 cccacccccc cactcctcgg ggccaataat tcttaataag gtatgatgtt aatacctcta
     3661 ggaaattgcc agggattcag gtgacagtgt gtgatgaaaa cggtgtattg tgtgctggcc
     3721 tgactgcatt accatgtttg tcttctattg tctaatgatt tcttatttct taaatgctgc
     3781 tgataaaatt attgatataa tacatttttg acagtagtag catttttgtg tataatgtat
     3841 agattttatt ggtttttact cagaagcact ctagattagt gtttcttgaa ctgcaatttg
     3901 aagccttctt tgggaatgtt agattgagga gtatttctct taaatacaaa tgccttttca
     3961 tctagcaaac caaaaattat atcttaatat tgaatgccat cactcggggt cggggtggga
     4021 gtaaattctg agataactcc agagttttca aggcatacaa ggtatgtgga atagcacaga
     4081 ttcagaatac atgatacata cgtataaaat gaaacatttc actttgaagt atttcttcaa
     4141 caaaacatgt cctgtctgcc tacttgctaa tgtgaaatta tgaaaggatt tattgaaata
     4201 tatgttcagt ttgaggtgta attggtaaga atgccaggtg ttgttcttct gccctcttaa
     4261 agaagtgtac ttaaaactat tgtgataatt catctctgat aaaactttgt tctggccttc
     4321 aaggctctta cagcttaatg ttttaaatgt ctaaggcatc aaataaattg agggcatttt
     4381 aataaaaaaa aaaaaggaaa actaaatgcc tttctttgtt aaagcaaaag gccaaaaata
     4441 aaaaataaaa ttaaaacccc ccccaaaaaa acccaaaagt cttactccta aagaagaatt
     4501 cttacctcta aggaggaatc cttccctctt gaggaaatac cactttttga gtgacattag
     4561 aaatcatcac aaggaaacat ttcaaagaaa ggatcatttt tttttcagga gaaagaggaa
     4621 taacaatttc attaacttcc tcaataggaa aggctagtct gaattattaa atattctgga
     4681 ttatatcttt ttttaatgat atagttttat tacttcagga atatttggta agaaccaggt
     4741 aaacctattg tttaagattt gttctttttt gttaatagac ctatgattag tatggtggaa
     4801 gcccaaaaag cagtctaatt tttatgataa acttacagac tgtggaaaga tgaatttctg
     4861 ccccaaacct tggataagtt gtcattaatt taattgtaga gatttttcta gtgaacttgt
     4921 acttcactgg aacaaatctt tagatctttt aaagcacaat ttatttatgt ttttaaaaac
     4981 agtcttaaaa ctgatcgatc atacatttgc tgtgtccacc ttacaatctt ttacattcta
     5041 ttagtacaga tagtgtttcc tttgctcctt ttcctgacca gttgttattg gtgtgaccat
     5101 tttcttttta ggatcgatga aacattttgc tgtgtatgct gtgtcatgta ttagaacctc
     5161 ttataatatg gctcattttt acatggtttc tgatttacca tgtgttaaat attccttaaa
     5221 atacatctag taacactata tagtaaggtt agattttaac ctaagtattg aatcttgtct
     5281 tataatgcca attttattgg agctttgttg catttatttt tggctctatg gtccataaat
     5341 gggagttaat agcaaacgtt ttattaaaaa tgtcttagct ttcccccaaa ttatatcatt
     5401 ttttagaagt tttatccttc tggggtgttt ttggctttct gagctttaag atattaatga
     5461 attttttcca catacttcat gggtttggtt ttaagtatga ggttaaaatt gtggatttta
     5521 aaaagctaat aaatacttga agtgaatata tagagagagc cagatttccc caaaactctg
     5581 tgagctgtgt gtgttttgtg tagaatatta gccataatta acttttccct tttttttttt
     5641 ggagacaggg tcttactctg tcacccagga tggagtgcag tggtgcggtc tcagctcact
     5701 gcagcctcga cctcctgggt tcaagagatt ctcgtgcgtc aacctcccaa gtagctggga
     5761 ttacagatgc ctgccaccat gcccagctaa gttttgtatt tttttttttg tttgttttag
     5821 taaagatggg atttcaccat gttggccagg ctggtctcaa actcctgccc tcaagtgatc
     5881 ccacccacct cagactccca aagtgctggg attacaggtg tgagccacca ctcctgggaa
     5941 ataattaact ttttcacata gaaaatagat cacagataaa agctcctcac atattttgaa
     6001 ggtcaaaaaa aaatttgctg ttgctaacaa ttgcctttga accatgaaag aggaaaaact
     6061 aactttattt gagcaacttg tcatttgaga cttaaactaa cttatcaaac ttagcagaat
     6121 taacttgtga gtattgactt aagtgaagtt agtttatata ctttactatt ctgtcatgca
     6181 aataaaatgg cttagagcaa gttcatacac agttactttt atctacaaat gtagaaatct
     6241 tacctatttg aaatttacat tattccctga aactatgcct attgagagag agtttataga
     6301 agtattatct gagaaacctg agtttgattt tctttttgtg acattcctgt taatgtcact
     6361 tttaaacaag ccacttaacc tatgtcattt tcagtttctt ctgtaaaatg gcgataatga
     6421 tacctaccag tattgttacg aagttcaaat aggccctatc cacagcactt agtaggtgtt
     6481 aaatgaatgc gtcttgttac ttgaacaaga agtggttagt tatgatagtt tatttttttc
     6541 tgacaaattt actgtctttt ataaaaagaa atagctagga tttcctgtgt tatcctgcca
     6601 tttttataat caaacaaaat tattaatatt ctccttagac taaatcatta atttaacaaa
     6661 tctcaagcat ctactatatg ccaagaagta ttctaggcct gaatatattg cagggcacga
     6721 agcaagacag taatttcgaa cctttatggg atttttattt taattggtta gtaaaatagt
     6781 ttatcatttg gtgacaaggt tatatgggga aagtaagttg aggaaggggc ccagagagtg
     6841 tgtgaggata ggattactat taagtagcat gtacactgat atgtgacatt gagcagaggc
     6901 ctgaggaggt gaatggttgt gcagtgtgga tatctgggag gagagcattc caggcagaag
     6961 gaatattaca tgctaaggcc ctgagctggt acagagtgta aagaaacagt aaagagatca
     7021 gtgtgactgt agtgaacaat aatagcattt tagggagaat tagaaaatga actgagagag
     7081 attgggagta ataatcctgc ctgattgtag gtaattacgt agggcattat agccctttct
     7141 caagagtagg actttactga gtgagctagc aagcaattag gattcagatc aaagcaagga
     7201 tggaagcagg tagtagtagg aagataatat ggtaaatcca ggtgacaaat ggtacaattt
     7261 gagattaaga gtaatggaaa taataagtgg tcagattctg aatgtatttt ggtggtagca
     7321 ccaaaaggat atgctgatag aatggatgtg aggtatgaaa aaaagaatga tcaaggtttt
     7381 tggcttgaag aatgaagtgg gcattaataa gatggcgaag actcagagga gcaggcttaa
     7441 gcgggtagat tgagagttta cttttggata tgttatggta gacttgtctg tcagacgtcc
     7501 aaaggaaaat gtagagtggg cagttggata tttgaacctg gaaaagtaga ggtctgggct
     7561 ggagataaat atatttggaa gtcatcagtg tgtaatctta ttttaagtca ggacatggat
     7621 gaaaccactt agagagtaag tgtagccaga ggatagaact taattgaaga catatgatcg
     7681 atgaaattca aggccaactt ttgtgatttt tcaaatagaa ataaaatgtt aacattttct
     7741 accacacata tacaaaccat tactttctat agtgttgtag aagcactaag atttaaaaag
     7801 caagattgat aatagttgcc aaatatgtaa acaaatggca aattaaattg aggagggagt
     7861 gtcactttat tcttggaata aagtttttgt tttgaagtaa tacagttttt tatattaaga
     7921 ataacaacat ctggagtgct agtttctttt tctcattgtt ggaaattgct attccaattc
     7981 aatttgacag ttttagcact agagggcagg cttgcttcta gattgtgaag agttctacat
     8041 gaattaatat agccaaacag cgcaggttga acatccttaa cttgaaaatc tgaaattcaa
     8101 aatgcttcaa aatccaaaac tttttgagtg ttgacatgat gccacaagtg gaaaattcca
     8161 cacctgacct catatgatgg gtcacagtca aaatgcaggt gtacaacagt ttactctgtt
     8221 cccaagagaa aaataaaatt agctgcaggc tatgtgtaaa aagtgtatat gaaacataaa
     8281 tgaatttcat gtttagactt gggtcccatc cccaaggtat cttattatga atatgcaaat
     8341 attcaaagat caaaaaaaag aaatctaaaa cacttccggt cccaagcatt ttggataaga
     8401 gatattcaac ctgcacctac attcaatctc aattaatgaa tgcagttgtt tcttatgtga
     8461 caggtttgcc atctactatg tttttttcta gtgctaggaa agcataaaat aaggctaatt
     8521 gattttagta catctaggac tggcagttga cgctaatata catttgaata taaatgtata
     8581 tttatattcc aaggagtttc caaggaaact caagtgtagc ttaattatac tcagatttga
     8641 tatagagggt cttccaccac atatatgaat attaaagcca gtcagaagaa atcctggaca
     8701 tgaaaacatt ttttcaggaa ataattaact atgtgctggg tattattata tgagcgtggc
     8761 tacaaagatg aataaaatat agtccctgcc taaataagct ttgttgtagc atcttacagc
     8821 acttaatatc ttgtgctgta tctatttaaa tgtctttttc ccttcaataa tagaaatcct
     8881 gactcactta tttttggtgc ctccaagtgc ccatcacaca attcagttta cagtgtttga
     8941 acatgtgaat aatgagagag agaaaaataa gaaaacggtc agggaattgg ggcctttgaa
     9001 aaaacttatt ttcacatttt tacccttaga gagatgcttt tcctattaac acgtctaaag
     9061 attatggaga caattctaac cttctatctt tgctcccact acttcttcaa gcagacttta
     9121 accccattcc tcaagagcag ttattggcag ccatagagag tgggaggtca agcctttttt
     9181 agtcattgga gtaaacagga gacatagatg tggaaattgg gaggttctct ctatctggct
     9241 tacgtcagtt tgagaagttt gacatgaaat ggaaccttct ggaatgaggt tctttttcct
     9301 gcttttcacc tggaaagttt tcccatacat ggtcctcttg cacccctagt ccttttaggg
     9361 tacctatttc tgtgtgctag ttctttcatt tccttgtctg taacatgagg ataataatag
     9421 tccttatcta atagcattat tttgaggatg aaatgaaata caggcagtaa atattagtta
     9481 ctctttgcta tctggaaaaa catcaattga acctcatagg atggacttgt gttcaataag
     9541 tgttatatat agtagagtca aatggaaaaa ttgaaccact cagatacatt tttttcattg
     9601 atacataata gatgtattta tttggggggt acatgtgata atatgatata ttcatataat
     9661 taaaattcat tgatacatga tgtatttatt tgggggtaca tgtgatagta tgatatattc
     9721 atataattaa aattcattga tacataatgt atttatttgg ggggtacatg tgatagtatg
     9781 atatattcat ataattaaat gaagataatt gggatatcca tcaccttaaa tatttatgtt
     9841 ttctttacac taggaacatt cgaattctct ttagctattt tgaaatgtac aatagtttag
     9901 tgttaataga tcagtcatcc tactgatata tcgaacacca ggtcttattt cttctgtcta
     9961 catagacata tttagaaatg caaatctctt aggaaattaa tctgtaatgt caatgatgca
    10021 cactgatttg tattacttgc tttctttgta ggcagtatag tgaaatggtt cattaaatgg
    10081 atgctaaata tgactaaatg atcaatttac agtgtagttg gcaccaagta ataaatgctc
    10141 agtaacatag ctactatggc tgtctttaaa agtggattgg tttgacttca taggaaggac
    10201 tctattttta aaaaagagaa atattaaaaa ataaaaagcc aaaattctat gatgtcttat
    10261 tacattgagt ctcttaaatc agtttttttt taattaagta gcaggaccct attgcaaaat
    10321 aagttttacc tagaagcaca gtatttaaaa cagaaagcta aaatactctg gttaagtggg
    10381 aagctccctg tcttcctcgc tgctagtatt atttctgaaa aacctcaaag gaactccttg
    10441 ctctctgaat agcaggtttt aaaaaccact acttgcacat ttgcaccttg acatcaaaca
    10501 tgtgaacaaa gtaatagagt gagaaaactg aatttctatt taggaatttc tattttattc
    10561 tctctataaa aagccaagaa gtggttggtg tttggaataa catgaaaaat attccaagta
    10621 tctttggaaa tacttacact agaactataa ggtttatgat aattctatct tagattcatt
    10681 atctagagct ggtctaaaaa aggatgatat gtaagaacta ccaagagaga cttggagtag
    10741 atacaagaat gaaggaatga atcaatatcc aataaatatc cataggccca ggctttgcta
    10801 tgccctctta ttacacagga taaaacagtg tagttgttag tttctacctt tgaagattat
    10861 gatccttaaa aaggaaacag ctctgtgaac agagaccatg ttattcatag gtgttcctca
    10921 cttgtttcca ccacataata ggtatttaat gaatgaaaaa attcacgtct ctctttgatc
    10981 ctagactgca agttacttaa aagaaatgga ccgtatttta cttatctctc atggcctgca
    11041 aattgagttt gcttactgaa ttaatgaatg aatgaccaat tgtacacata tgtctgtaaa
    11101 acgtttacat atccacattc ccattagact gagattcttg agggcagaaa ccatgtcttc
    11161 agctctcagc atggtgcctg actcatggta ggttgtcaaa agcatttgca gaactgaata
    11221 aaacttacgt ttaagtagta ctggagcagt ctcacataac cgggcacagt gtcttctaaa
    11281 tagacactaa aattatttca taattaaaga cagatctcac taagcatttt ctgagttagt
    11341 gccagttgaa taatatgaca ataagctata ggagttcaga ggagagtgag ttctgtggac
    11401 tgaaatagtc aaagtggatt ttcagagcag atgagacgcg atgaaggctg gggctacttt
    11461 ggtgttttaa gaacattaat taggcagcag gaaaatctga ggacagggag actagtaagg
    11521 aaggctggaa agagccctgg gtttggagtt cagagatctg tgttcagagt cctttgcttc
    11581 ttagtgacta tgtgaccttg agaagataac ttgagctccc tttttcctct tctgtgaggt
    11641 agaggtaaga ttgatgtgca tattgatatt gtacttaaaa gtgtttgtaa aatgctaagt
    11701 aaaacctaat gctatgcttt gaatgtagtg ggttccaaat atttgtttaa tgacaaagaa
    11761 taaatgatat tgactaatga aaataatgga gccactggaa tgtcacctcc tgcatctgcc
    11821 agtaaacctg ttaacacagg ggtggcgggg ggggggggga agtaaatatg tgaagccacc
    11881 aaatctcatg atcaggagaa aaactttgga actcccatga gactccaaag ttatgtcaca
    11941 ggtgaataag tcatgaactt ttcatgctga tatcatttct aaggttgctg ccttgtcaga
    12001 agaagcaaac cacaagataa accatgcaga aaatcttttt ttaaaaaagt ttcctgaagg
    12061 atggatggtt catttgttct gcttgtcact ttacttacta atgagtcatt tttttcctta
    12121 accagtaatt tgttttaaat ttggtcataa gatggtatgt tgactagtcc aatgttacaa
    12181 aataattgga aaaaagatga cagaccacca actgaataag ggaaatctgg ttcttgaact
    12241 tccaatgtat gaattggtga tggtaagctt agtctttatg atgtaagatt ggatttattg
    12301 gaacttcttt atgtccagaa gttgcagcac ctttctttgt ctctggaatg cccaagatct
    12361 ctactgcatc ctgtagacat ggagggtttc cccccttatt ctgggtctga ttatttttaa
    12421 agaaacaccc tttccttacc catcatggag acactactaa ccttgtatat acttttatta
    12481 gagtgctgat aacaccatat tacagtagca tacttacata tctgttttct taactgtgag
    12541 ctccttgaga gcagggactg tgtgtttttg cttttatttc accagtactc agaactatcc
    12601 acagtataga aaagcactca ataaatggaa taaatgaaaa tgaatagata aataaatgat
    12661 ttcacttgta taaga
//
