LOCUS       NM_001040402            4222 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens defective in cullin neddylation 1 domain containing 4
            (DCUN1D4), transcript variant 1, mRNA.
ACCESSION   NM_001040402
VERSION     NM_001040402.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Keuss,M.J., Thomas,Y., Mcarthur,R., Wood,N.T., Knebel,A. and
            Kurz,T.
  TITLE     Characterization of the mammalian family of DCN-type NEDD8 E3
            ligases
  JOURNAL   J Cell Sci 129 (7), 1441-1454 (2016)
   PUBMED   26906416
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Monda,J.K., Scott,D.C., Miller,D.J., Lydeard,J., King,D.,
            Harper,J.W., Bennett,E.J. and Schulman,B.A.
  TITLE     Structural conservation of distinctive N-terminal
            acetylation-dependent interactions across a family of mammalian
            NEDD8 ligation enzymes
  JOURNAL   Structure 21 (1), 42-53 (2013)
   PUBMED   23201271
REFERENCE   3  (bases 1 to 4222)
  AUTHORS   Khadka,S., Vangeloff,A.D., Zhang,C., Siddavatam,P., Heaton,N.S.,
            Wang,L., Sengupta,R., Sahasrabudhe,S., Randall,G., Gribskov,M.,
            Kuhn,R.J., Perera,R. and LaCount,D.J.
  TITLE     A physical interaction network of dengue virus and human proteins
  JOURNAL   Mol Cell Proteomics 10 (12), M111.012187 (2011)
   PUBMED   21911577
REFERENCE   4  (bases 1 to 4222)
  AUTHORS   Kurz,T., Ozlu,N., Rudolf,F., O'Rourke,S.M., Luke,B., Hofmann,K.,
            Hyman,A.A., Bowerman,B. and Peter,M.
  TITLE     The conserved protein DCN-1/Dcn1p is required for cullin
            neddylation in C. elegans and S. cerevisiae
  JOURNAL   Nature 435 (7046), 1257-1261 (2005)
   PUBMED   15988528
REFERENCE   5  (bases 1 to 4222)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK124346.1, D87466.1 and BC053897.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001040402.2.
            
            Transcript Variant: This variant (1) has an alternate exon in place
            of the first exon compared to variant 3. The resulting isoform (1)
            has a shorter and distinct N-terminus compared to isoform 3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX537944.1, SRR1660803.94473.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334635.10/ ENSP00000334625.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AK124346.1         154-190
            38-2227             D87466.1           1-2190
            2228-4222           BC053897.1         2139-4133
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q12"
     gene            1..4222
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /note="defective in cullin neddylation 1 domain containing
                     4"
                     /db_xref="GeneID:23142"
                     /db_xref="HGNC:HGNC:28998"
                     /db_xref="MIM:612977"
     exon            1..115
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..969
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DCN1-like protein 4; DCUN1 domain-containing protein 4;
                     defective in cullin neddylation protein 1-like protein 4;
                     DCN1, defective in cullin neddylation 1, domain containing
                     4"
                     /codon_start=1
                     /product="DCN1-like protein 4 isoform 1"
                     /protein_id="NP_001035492.1"
                     /db_xref="CCDS:CCDS33982.1"
                     /db_xref="GeneID:23142"
                     /db_xref="HGNC:HGNC:28998"
                     /db_xref="MIM:612977"
                     /translation="MHSDAAAVNFQLNSHLSTLANIHKIYHTLNKLNLTEDIGQDDHQ
                     TGSLRSCSSSDCFNKVMPPRKKRRPASGDDLSAKKSRHDSMYRKYDSTRIKTEEEAFS
                     SKRCLEWFYEYAGTDDVVGPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQE
                     WLKGMTSLQCDTTEKLRNTLDYLRSFLNDSTNFKLIYRYAFDFAREKDQRSLDINTAK
                     CMLGLLLGKIWPLFPVFHQFLEQSKYKVINKDQWCNVLEFSRTINLDLSNYDEDGAWP
                     VLLDEFVEWYKDKQMS"
     exon            116..186
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            187..226
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            227..341
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            342..433
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            434..504
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            505..596
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            597..705
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            706..810
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            811..913
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     exon            914..4222
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4193..4198
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /note="hexamer: AATAAA"
     polyA_site      4222
                     /gene="DCUN1D4"
                     /gene_synonym="DCNL4"
                     /note="major polyA site"
ORIGIN      
        1 agcttcgagc cgaggtgcag tgagctggtg gggggaccgc gaggcgagcg cgggagcctg
       61 ggcggcgagc cgggtgtgag ctgcctgaaa atgcactcgg atgccgccgc tgtcaatttt
      121 cagctgaact ctcatctctc aacactggca aatattcata agatctacca cacccttaat
      181 aagctgaacc taacagaaga cattggccaa gacgatcacc aaacaggaag tctgcggtct
      241 tgcagttctt cagactgctt taataaagtg atgccaccaa ggaaaaagag aagacctgcc
      301 tctggagatg atttatctgc caagaaaagt agacatgata gcatgtatag aaaatatgat
      361 tcgactagaa taaagactga agaagaagcc ttttcaagta aaaggtgctt ggaatggttc
      421 tatgaatatg caggaactga tgatgttgta ggccctgaag gcatggagaa attttgtgaa
      481 gacattggtg ttgaaccaga aaacgtagtt atgcttgtcc tagcttggaa attggatgca
      541 caaaacatgg gttattttac tctacaggag tggttaaaag gaatgacttc tctccaatgt
      601 gatacaacag aaaaactcag aaatactttg gattacttaa gatcattctt aaatgattct
      661 acaaacttta aacttattta cagatatgcg tttgactttg cacgggaaaa ggaccagcgc
      721 agcctagaca taaacactgc caagtgcatg ttgggactgt tattaggaaa aatctggccc
      781 ctttttccag tttttcacca attcttagag caatcaaaat acaaagttat taataaagac
      841 cagtggtgca atgtcctaga gtttagcaga acaattaatc ttgacctcag caactatgat
      901 gaagatggag catggccagt tttgttggac gagtttgtgg agtggtataa agacaaacag
      961 atgtcctagg actttatgca tagcagcgag agagtcactg ttaccacagt tttgtcaccc
     1021 attagccata aattgctgtt tgtatcaaag cgcatgctgc ttctcttgca ctgtttccct
     1081 ttcgcaggga catgttggtg tttgctattg aattggccag ctctgcttgc tgtgtggcat
     1141 tgttctcttg gaaggctgct ttgcagtttg tatttacact acagattggt gaatttgcca
     1201 acgtcctcac tgtgattatg tgtatattgc tgtttaaatt ttgtatatgt gtataaaagg
     1261 aaaaaggttc acctagagat tatttctgaa aaatgtattg taaaaataat tttgtggcat
     1321 ttctagtccc tttttttgaa tgaaccaatt atactttatt tggtctccta tgtagcattt
     1381 cagaaaacaa gagaaaactg ttaccatgaa caaacattgc cagaattaac cttactgttt
     1441 aagaggccaa cttctggaag gaggtaggag tcataacttt ttagaggcat atgccaaata
     1501 tcatttggta tacttaacaa tattagtgtt ttaaaatgat gagttataat tatttgaaca
     1561 tatagatatg taacatgcca caaatcattt ctaccatgca aggtgtataa gttgtttatt
     1621 ttttagtgtt aaaactataa tagcttgaat ataggtacca atgaacaaat tcaaattgca
     1681 cctcttttct taaaagaatg ggatttaaac tcttataaac attctttaac ttttttgttt
     1741 gtttgttctc tttttttcct tttgcattct tctagccagt gattgatctg ctaatgcttt
     1801 ctttgccact ctaagtaaaa tttatttcac ctcctcaatg aaaacctcat ggttttgctg
     1861 gctgtttata actgcatcgc acttctagtt gtggcttgaa ttttcagtta agctttcatg
     1921 gtatgtaatt ttccagcctt ttgagaaaac aagcatacta taagtgagag ctgttttgtt
     1981 ttccttgttt gtttgtttca tgctaggctt ttcctggcag catgtccatt gcaggcagtg
     2041 gacaagaaac caccagcatt gagctaaccc agtacatgct aggacctgtc ctagaggggc
     2101 cacttttcat tacctgagtt atttgtacag aagggcaata gccattattt ttgtggatga
     2161 ggaaacaaga ataaacagaa tggtattttt aggtttgtat tttatgtctt tttttttttt
     2221 tttttttgcc attcttgagg aaatatagag atgacatgtt ttcaccccaa ctatctggtg
     2281 ctattgaatg actaattcag tccctaaagt tctgtgaaaa cacaaaagtc taatgatttg
     2341 agtgagtaaa aggtaatggt gcatttgaac aagtaaatgc tgtcgtggtc agcaagatcc
     2401 gtgatttgaa catgtgatga ctggaaaaag gtttgggtta tttggaactc tggctaaaac
     2461 ttctttcggg tgacatgtga tcgtttaaat ggcattaagt gaataaagca cacagacagt
     2521 gctactcttg accactattt taccatttct ttgcaaacag tgttcacatt ttcatatttt
     2581 ttccctaact aaaccaccaa agaaagaaat tttgtatgta tatacagtgt gtgtgtatac
     2641 aaaatcatga tatagtagaa tgcaactact ttctttttct accaaacgaa aggttttatt
     2701 tgctgtgaaa taaaccagaa gtttaaaaaa ccctgtagtg attaagcata cttaaccact
     2761 ccttatttgt agattcactt tcaaccttaa aaattaatac cagtttgcat aaaccaatat
     2821 ctgaaaagaa caggaaatgt taatggcaag caacagctat taatactgat gtgatggatg
     2881 catttgtttt gcagtggtga ctggcctagg caggtttgga tctgtgaaga attgattcat
     2941 tttcaaaatt attccataaa gttaaaaagt tacactttaa aggcaacagg tcatacagtt
     3001 ctttaaatct gatcaactgt agctttattt aaagcaaata taaatcatag atgaagttat
     3061 tttaaacact atgttagaat atagagattt ttaaaaaatg ctgataagca cagttaattc
     3121 taaaatgaga ggattgttac aggagggaga tgttacagtt aatccacaat caacattttg
     3181 atggtaggga aaaaactgcg taaaaactat tgcattatgt atgataagaa agtaagtatt
     3241 ccaaagggat aatcttgcat attaagaaaa ctccaaataa tctttaaact gccttgaaaa
     3301 ataaacctct ttgactgctg agcggcagag tgcttaccct tgattgtctt gattttatat
     3361 atttatttct aaggggagaa aaaggggtca gacagagtaa tatgatacat tttcttaaaa
     3421 acttctctaa gttgcacaaa actgaacaat catcaagcac ttatttactg gctaatgttg
     3481 aataaattag ggtgccttca tctgggtttt ctttagtacc taagtctaca accctttctc
     3541 tttttctatt gtgtttcgaa ctccacattg caatactctg ttgtcatgga atacaacctt
     3601 tagatgttta ctgctgaagt aggtcagaaa ttgtattatt tgatttgcct caaacatcca
     3661 ctgtaatgtt acatgcacct tttttgaagc atgaactctg aattatgttt ttataaatct
     3721 gactaggcaa acctagattc tggtttcaca atggatttat tttctctctc agtctccatt
     3781 ttaacagtgc ttttgaagtt tatacagaaa gctttaaaag ttagtttgtg cccttggttt
     3841 ttattcttaa aactgctaaa atacctctgt aagccttatc ctttattctt tcatatgttg
     3901 tataataaat gtatagattt cattgaccaa ctaaaatgtt gggtgtctgt aaatgagacc
     3961 aaaacgtggg ttgctttttt cataaaataa tttctattgg gggttactgt tcaatgacag
     4021 caggtaacct ataactgtga atgcttcgtg tcgtcagtat ttgcattaca ttcataaaag
     4081 tgtgcaagtc ctgtgactcc cagcttaact gaaatactgt tatgccacct aacttgagta
     4141 cagcaaactg gttttaggtt tcaatgacat tgatgtaaaa tgatatccca tgaataaaaa
     4201 gtatttgtgt ttggtttcag aa
//
