LOCUS       NM_007234                828 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens dynactin subunit 3 (DCTN3), transcript variant 1,
            mRNA.
ACCESSION   NM_007234
VERSION     NM_007234.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Su,X., Li,H., Chen,S. and Qin,C.
  TITLE     Study on the Prognostic Values of Dynactin Genes in Low-Grade
            Glioma
  JOURNAL   Technol Cancer Res Treat 20, 15330338211010143 (2021)
   PUBMED   33896271
  REMARK    GeneRIF: Study on the Prognostic Values of Dynactin Genes in
            Low-Grade Glioma.
REFERENCE   2  (bases 1 to 828)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 828)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 828)
  AUTHORS   Boldt,K., van Reeuwijk,J., Lu,Q., Koutroumpas,K., Nguyen,T.M.,
            Texier,Y., van Beersum,S.E., Horn,N., Willer,J.R., Mans,D.A.,
            Dougherty,G., Lamers,I.J., Coene,K.L., Arts,H.H., Betts,M.J.,
            Beyer,T., Bolat,E., Gloeckner,C.J., Haidari,K., Hetterschijt,L.,
            Iaconis,D., Jenkins,D., Klose,F., Knapp,B., Latour,B.,
            Letteboer,S.J., Marcelis,C.L., Mitic,D., Morleo,M., Oud,M.M.,
            Riemersma,M., Rix,S., Terhal,P.A., Toedt,G., van Dam,T.J., de
            Vrieze,E., Wissinger,Y., Wu,K.M., Apic,G., Beales,P.L.,
            Blacque,O.E., Gibson,T.J., Huynen,M.A., Katsanis,N., Kremer,H.,
            Omran,H., van Wijk,E., Wolfrum,U., Kepes,F., Davis,E.E., Franco,B.,
            Giles,R.H., Ueffing,M., Russell,R.B. and Roepman,R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 828)
  AUTHORS   Kuzma-Kozakiewicz,M., Kazmierczak,B., Chudy,A., Gajewska,B. and
            Baranczyk-Kuzma,A.
  TITLE     Alteration of Motor Protein Expression Involved in Bidirectional
            Transport in Peripheral Blood Mononuclear Cells of Patients with
            Amyotrophic Lateral Sclerosis
  JOURNAL   Neurodegener Dis 16 (3-4), 235-244 (2016)
   PUBMED   26954557
  REMARK    GeneRIF: Study found that in peripheral blood mononuclear cells the
            median expression of KIFC3, KIF1B, and KIF5C was much lower than
            the expression of dynactin subunits DCTN1 and DCTN3, in both
            sporadic amyotrophic lateral sclerosis and healthy cases
REFERENCE   6  (bases 1 to 828)
  AUTHORS   Gupta,G.D., Coyaud,E., Goncalves,J., Mojarad,B.A., Liu,Y., Wu,Q.,
            Gheiratmand,L., Comartin,D., Tkach,J.M., Cheung,S.W., Bashkurov,M.,
            Hasegan,M., Knight,J.D., Lin,Z.Y., Schueler,M., Hildebrandt,F.,
            Moffat,J., Gingras,A.C., Raught,B. and Pelletier,L.
  TITLE     A Dynamic Protein Interaction Landscape of the Human
            Centrosome-Cilium Interface
  JOURNAL   Cell 163 (6), 1484-1499 (2015)
   PUBMED   26638075
REFERENCE   7  (bases 1 to 828)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   8  (bases 1 to 828)
  AUTHORS   Mills,D.R. and Jackson,C.L.
  TITLE     Assignment of p22 dynactin light chain (DCTN3) to human chromosome
            region 9p13 by radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 92 (1-2), 166 (2001)
   PUBMED   11306820
REFERENCE   9  (bases 1 to 828)
  AUTHORS   Karki,S., Tokito,M.K. and Holzbaur,E.L.
  TITLE     A dynactin subunit with a highly conserved cysteine-rich motif
            interacts directly with Arp1
  JOURNAL   J Biol Chem 275 (7), 4834-4839 (2000)
   PUBMED   10671518
REFERENCE   10 (bases 1 to 828)
  AUTHORS   Karki,S., LaMonte,B. and Holzbaur,E.L.
  TITLE     Characterization of the p22 subunit of dynactin reveals the
            localization of cytoplasmic dynein and dynactin to the midbody of
            dividing cells
  JOURNAL   J Cell Biol 142 (4), 1023-1034 (1998)
   PUBMED   9722614
  REMARK    Erratum:[J Cell Biol 1998 Oct 19;143(2):following 560]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA212054.1, BC107697.1 and
            CN257020.1.
            
            On Nov 22, 2018 this sequence version replaced NM_007234.4.
            
            Summary: This gene encodes the smallest subunit of dynactin, a
            macromolecular complex consisting of 10 subunits ranging in size
            from 22 to 150 kD. Dynactin binds to both microtubules and
            cytoplasmic dynein. It is involved in a diverse array of cellular
            functions, including ER-to-Golgi transport, the centripetal
            movement of lysosomes and endosomes, spindle formation,
            cytokinesis, chromosome movement, nuclear positioning, and
            axonogenesis. This subunit, like most other dynactin subunits,
            exists only as a part of the dynactin complex. It is primarily an
            alpha-helical protein with very little coiled coil, and binds
            directly to the largest subunit (p150) of dynactin. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.555144.1,
                                           SRR5189661.24228.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000259632.12/ ENSP00000259632.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DA212054.1         29-35
            8-821               BC107697.1         5-818
            822-828             CN257020.1         602-608
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..828
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /note="dynactin subunit 3"
                     /db_xref="GeneID:11258"
                     /db_xref="HGNC:HGNC:2713"
                     /db_xref="MIM:607387"
     exon            1..127
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..592
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     dynactin light chain; dynactin complex subunit 22 kDa
                     subunit; dynactin 3 (p22)"
                     /codon_start=1
                     /product="dynactin subunit 3 isoform 1"
                     /protein_id="NP_009165.1"
                     /db_xref="CCDS:CCDS6560.1"
                     /db_xref="GeneID:11258"
                     /db_xref="HGNC:HGNC:2713"
                     /db_xref="MIM:607387"
                     /translation="MAGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVAL
                     GNISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLE
                     QVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQDQCVEITEESKALLEEYNKTTML
                     LSKQFVQWDELLCQLEAATQVKPAEE"
     exon            128..212
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     exon            213..299
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     exon            300..383
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     exon            384..442
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     exon            443..502
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     exon            503..828
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      806..811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /note="hexamer: AATAAA"
     polyA_site      828
                     /gene="DCTN3"
                     /gene_synonym="DCTN-22; DCTN22"
                     /note="major polyA site"
ORIGIN      
        1 gtttcctggc cgaggtgggt cggtagtagc gatggcgggt ctgactgact tgcagcggct
       61 acaggcccga gtggaagagc tggagcgctg ggtgtacggg ccgggcgggg cgcgcggctc
      121 acggaaggtg gctgacggcc tggtcaaggt gcaggtggct ttggggaaca tttccagcaa
      181 gagggagagg gtgaagattc tctacaaaaa gattgaagat ctgatcaagt acctggatcc
      241 tgagtacatc gaccgcattg ccatacctga tgcctctaag ctgcaattca tcctagcaga
      301 ggagcagttt atcctttccc aggttgcact cctggagcag gtgaatgcct tggtgcccat
      361 gctggacagt gctcacatca aagccgttcc tgagcatgct gcccgcctgc agcgcttggc
      421 ccagatccac attcagcagc aggaccagtg tgtggaaatc actgaggagt ccaaggctct
      481 cctggaggaa tacaacaaga ctacaatgct tctctccaag caattcgtgc agtgggatga
      541 gctactttgc cagctagagg ccgccacgca agtgaagcca gcagaggagt gatagctgct
      601 ccccatccca aagtgggcct gggcagtcag gctccagggc cctatgccaa cctgcctttg
      661 ttacaaggca gaggaagctt tgtatttatt ggcttcaagg cccacctctc tgtactctgg
      721 gctctaaagt tggaggtcag gttacctgag gtttgcaatt tgcaacaccc accctccccc
      781 caatcagtgt tcttatttca gtgacaataa accatagaga tgactgga
//
