LOCUS       NM_001261413            1973 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 3,
            mRNA.
ACCESSION   NM_001261413
VERSION     NM_001261413.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Xu,S., Xing,J., Li,K., Qiao,L., Zhang,C., Ren,Y. and Liu,Y.
  TITLE     Pan-cancer analysis of DCTN2 and its tumour-promoting role in HCC
            by modulating the AKT pathway
  JOURNAL   J Cell Mol Med 28 (11), e18450 (2024)
   PUBMED   38842133
  REMARK    GeneRIF: Pan-cancer analysis of DCTN2 and its tumour-promoting role
            in HCC by modulating the AKT pathway.
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Li,Y.F., Shi,L.J., Wang,P., Wang,J.W., Shi,G.Y. and Lee,S.C.
  TITLE     Binding between ROCK1 and DCTN2 triggers diabetes-associated
            centrosome amplification in colon cancer cells
  JOURNAL   Oncol Rep 46 (1) (2021)
   PUBMED   34080666
  REMARK    GeneRIF: Binding between ROCK1 and DCTN2 triggers
            diabetesassociated centrosome amplification in colon cancer cells.
REFERENCE   3  (bases 1 to 1973)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1973)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1973)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 1973)
  AUTHORS   Eckley,D.M., Gill,S.R., Melkonian,K.A., Bingham,J.B., Goodson,H.V.,
            Heuser,J.E. and Schroer,T.A.
  TITLE     Analysis of dynactin subcomplexes reveals a novel actin-related
            protein associated with the arp1 minifilament pointed end
  JOURNAL   J Cell Biol 147 (2), 307-320 (1999)
   PUBMED   10525537
REFERENCE   7  (bases 1 to 1973)
  AUTHORS   Berrueta,L., Tirnauer,J.S., Schuyler,S.C., Pellman,D. and
            Bierer,B.E.
  TITLE     The APC-associated protein EB1 associates with components of the
            dynactin complex and cytoplasmic dynein intermediate chain
  JOURNAL   Curr Biol 9 (8), 425-428 (1999)
   PUBMED   10226031
REFERENCE   8  (bases 1 to 1973)
  AUTHORS   Starr,D.A., Williams,B.C., Hays,T.S. and Goldberg,M.L.
  TITLE     ZW10 helps recruit dynactin and dynein to the kinetochore
  JOURNAL   J Cell Biol 142 (3), 763-774 (1998)
   PUBMED   9700164
REFERENCE   9  (bases 1 to 1973)
  AUTHORS   Echeverri,C.J., Paschal,B.M., Vaughan,K.T. and Vallee,R.B.
  TITLE     Molecular characterization of the 50-kD subunit of dynactin reveals
            function for the complex in chromosome alignment and spindle
            organization during mitosis
  JOURNAL   J Cell Biol 132 (4), 617-633 (1996)
   PUBMED   8647893
REFERENCE   10 (bases 1 to 1973)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB027733.1, BC009468.1 and
            DB569305.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_001261413.1.
            
            Summary: This gene encodes a 50-kD subunit of dynactin, a
            macromolecular complex consisting of 10-11 subunits ranging in size
            from 22 to 150 kD. Dynactin binds to both microtubules and
            cytoplasmic dynein. It is involved in a diverse array of cellular
            functions, including ER-to-Golgi transport, the centripetal
            movement of lysosomes and endosomes, spindle formation, chromosome
            movement, nuclear positioning, and axonogenesis. This subunit is
            present in 4-5 copies per dynactin molecule. It contains three
            short alpha-helical coiled-coil domains that may mediate
            association with self or other dynactin subunits. It may interact
            directly with the largest subunit (p150) of dynactin and may affix
            p150 in place. Multiple alternatively spliced transcript variants
            encoding distinct isoforms have been found for this gene. [provided
            by RefSeq, May 2012].
            
            Transcript Variant: This variant (3) lacks two exons in the 5'
            coding region but maintains the reading frame, compared to variant
            1. The resulting isoform (3) lacks an internal segment, compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ891494.1, BC000718.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000548249.6/ ENSP00000447824.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                DB027733.1         140-210
            72-1713             BC009468.1         1-1642
            1714-1973           DB569305.1         202-461
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..1973
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /note="dynactin subunit 2"
                     /db_xref="GeneID:10540"
                     /db_xref="HGNC:HGNC:2712"
                     /db_xref="MIM:607376"
     exon            1..165
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1335
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /note="isoform 3 is encoded by transcript variant 3; p50
                     dynamitin; dynactin complex 50 kDa subunit; epididymis
                     secretory protein Li 77; 50 kDa dynein-associated
                     polypeptide; dynactin 2 (p50); epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="dynactin subunit 2 isoform 3"
                     /protein_id="NP_001248342.1"
                     /db_xref="CCDS:CCDS58245.1"
                     /db_xref="GeneID:10540"
                     /db_xref="HGNC:HGNC:2712"
                     /db_xref="MIM:607376"
                     /translation="MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVE
                     HIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQK
                     YQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGP
                     DAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKF
                     SQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVL
                     DQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLV
                     TIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASI
                     DERMKKLGK"
     exon            166..234
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            235..331
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            332..393
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            394..492
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            493..653
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            654..798
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            799..864
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            865..903
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            904..981
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1053
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1156
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1248
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1973
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1693..1698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /note="hexamer: ATTAAA"
     polyA_site      1713
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /note="major polyA site"
     regulatory      1849..1854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
                     /note="hexamer: AATAAA"
     polyA_site      1873
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
     polyA_site      1973
                     /gene="DCTN2"
                     /gene_synonym="DCTN50; DYNAMITIN; HEL-S-77; RBP50"
ORIGIN      
        1 acttcctgct catctgccgc tccctttgcc gccgccttag cccgggaccc gaacccagcc
       61 tctcccctac ccgaacaccg gccccggctc caccgaggcc cgggtccccc agcccgtctc
      121 gccgccgcca tggcggaccc taaatacgcc gaccttcccg gcattgccag gaatgagcca
      181 gatgtttatg aaactagcga cctacctgag gatgatcaag cggagttcga tgcggaggag
      241 ctgacaagca caagtgtgga acacatcatt gtcaatccta atgctgccta tgacaagttc
      301 aaggacaaga gagtggggac aaagggactt gatttctcag atcgtattgg aaaaaccaag
      361 aggacaggat atgaatctgg agaatatgag atgcttggag agggtctggg agtgaaggag
      421 acaccccagc aaaagtacca gcgcctactg catgaggtcc aagagctgac aactgaagtt
      481 gaaaaaatca agacgacagt gaaggagtca gccacagagg agaagctgac ccctgtgttg
      541 ctggctaaac agctggcagc cctgaagcag cagctggttg cttcccacct ggagaagctg
      601 ctgggaccag atgctgcaat caaccttacc gaccccgatg gcgccctggc taagcgccta
      661 ctactgcagc tggaagcaac aaagaacagc aaagggggat cagggggaaa aaccactggg
      721 acccccccag atagcagcct tgtcacttat gaactacatt ctcggcctga gcaggacaag
      781 ttctctcaag ctgccaaagt cgcagaactt gaaaagcgcc tgacagagct ggagacagct
      841 gtacgttgtg atcaggatgc tcagaatccc ctttctgcag gtctacaggg agcctgtctc
      901 atggagactg tagagctgtt gcaagcaaag gtgagcgccc tagaccttgc agttttggat
      961 caagtggagg ctcggctaca gagtgtcctg ggaaaggtga acgagattgc caagcataaa
     1021 gcctctgtag aagatgcaga tacacaaagc aaggtgcacc agctatatga aactatacag
     1081 cgctggagcc ccattgcctc caccctccct gagctggtgc agagacttgt caccatcaag
     1141 cagctgcacg agcaagccat gcagtttggt cagctcctga cacacttgga taccacccag
     1201 cagatgattg ctaattcctt gaaggacaat accaccctct tgacccaggt gcagacaacc
     1261 atgcgtgaaa acctggccac agttgagggg aactttgcca gcattgatga acggatgaag
     1321 aagctgggaa agtgagcaca tttgggagct ggagaacagg ggttatccct acccctgtga
     1381 actctgttaa cagcttacat agggtttccc ctttactata actctagcat ccccatccca
     1441 tttgacactg ggggcaaggg ttcttcttgc atgtggggtt tatacccctc ccctgatgaa
     1501 tacagagtgg tagctagggg ttggttatca tcagaaggtg gtctcccctc aggcctgggg
     1561 gataaggacg tgggcccagc cacatgccaa ctcatgtcca atactgcttt gcctggtgtg
     1621 gggaaggatt gggtcttgtc ccccaacaca gcttctgtgg ctgactgtaa tactgtacaa
     1681 ctgtttctga ccattaaatg ctgttgtact ctgtgtggcc tctgctgtgt ttcctgggga
     1741 ggaagcagca ctaggatata gatattcatt cgtcataaca ggcaatctaa gccactctat
     1801 actacaagag atggatttaa attgtaacct gttcttacca aagaactaaa taaaaaatga
     1861 gtacagagcc agagccagag tttcaaaata ttctcatctg ttaaattaag agtgtctccc
     1921 atagaaaagc agtggaggcc ccacagggca agtacaaaac agaattaaaa ctc
//
