LOCUS       NM_014026               5427 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens decapping enzyme, scavenger (DCPS), transcript variant
            2, mRNA.
ACCESSION   NM_014026
VERSION     NM_014026.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Masoudi,M., Bereshneh,A.H., Rasoulinezhad,M., Ashrafi,M.R.,
            Garshasbi,M. and Tavasoli,A.R.
  TITLE     Leukoencephalopathy in Al-Raqad syndrome: Expanding the clinical
            and neuroimaging features caused by a biallelic novel missense
            variant in DCPS
  JOURNAL   Am J Med Genet A 182 (10), 2391-2398 (2020)
   PUBMED   32770650
  REMARK    GeneRIF: Leukoencephalopathy in Al-Raqad syndrome: Expanding the
            clinical and neuroimaging features caused by a biallelic novel
            missense variant in DCPS.
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Fuchs,A.L., Wurm,J.P., Neu,A. and Sprangers,R.
  TITLE     Molecular basis of the selective processing of short mRNA
            substrates by the DcpS mRNA decapping enzyme
  JOURNAL   Proc Natl Acad Sci U S A 117 (32), 19237-19244 (2020)
   PUBMED   32723815
  REMARK    GeneRIF: Molecular basis of the selective processing of short mRNA
            substrates by the DcpS mRNA decapping enzyme.
REFERENCE   3  (bases 1 to 5427)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5427)
  AUTHORS   Gopalsamy,A., Narayanan,A., Liu,S., Parikh,M.D., Kyne,R.E. Jr.,
            Fadeyi,O., Tones,M.A., Cherry,J.J., Nabhan,J.F., LaRosa,G.,
            Petersen,D.N., Menard,C., Foley,T.L., Noell,S., Ren,Y., Loria,P.M.,
            Maglich-Goodwin,J., Rong,H. and Jones,L.H.
  TITLE     Design of Potent mRNA Decapping Scavenger Enzyme (DcpS) Inhibitors
            with Improved Physicochemical Properties To Investigate the
            Mechanism of Therapeutic Benefit in Spinal Muscular Atrophy (SMA)
  JOURNAL   J Med Chem 60 (7), 3094-3108 (2017)
   PUBMED   28257199
REFERENCE   5  (bases 1 to 5427)
  AUTHORS   Milac,A.L., Bojarska,E. and Wypijewska del Nogal,A.
  TITLE     Decapping Scavenger (DcpS) enzyme: advances in its structure,
            activity and roles in the cap-dependent mRNA metabolism
  JOURNAL   Biochim Biophys Acta 1839 (6), 452-462 (2014)
   PUBMED   24742626
  REMARK    Review article
REFERENCE   6  (bases 1 to 5427)
  AUTHORS   Gu,M., Fabrega,C., Liu,S.W., Liu,H., Kiledjian,M. and Lima,C.D.
  TITLE     Insights into the structure, mechanism, and regulation of scavenger
            mRNA decapping activity
  JOURNAL   Mol Cell 14 (1), 67-80 (2004)
   PUBMED   15068804
  REMARK    GeneRIF: Structural and biochemical analysis suggests an
            autoregulatory mechanism whereby premature decapping mRNA is
            prevented by blocking the conformational changes that are required
            to form a closed productive active site capable of cap hydrolysis.
REFERENCE   7  (bases 1 to 5427)
  AUTHORS   van Dijk,E., Le Hir,H. and Seraphin,B.
  TITLE     DcpS can act in the 5'-3' mRNA decay pathway in addition to the
            3'-5' pathway
  JOURNAL   Proc Natl Acad Sci U S A 100 (21), 12081-12086 (2003)
   PUBMED   14523240
  REMARK    GeneRIF: m7GDP breakdown by DcpS should prevent misincorporation of
            methylated nucleotides in nucleic acids and could generate a unique
            indicator allowing the cell to monitor mRNA decay.
REFERENCE   8  (bases 1 to 5427)
  AUTHORS   Kwasnicka,D.A., Krakowiak,A., Thacker,C., Brenner,C. and
            Vincent,S.R.
  TITLE     Coordinate expression of NADPH-dependent flavin reductase, Fre-1,
            and Hint-related 7meGMP-directed hydrolase, DCS-1
  JOURNAL   J Biol Chem 278 (40), 39051-39058 (2003)
   PUBMED   12871939
REFERENCE   9  (bases 1 to 5427)
  AUTHORS   Liu,H., Rodgers,N.D., Jiao,X. and Kiledjian,M.
  TITLE     The scavenger mRNA decapping enzyme DcpS is a member of the HIT
            family of pyrophosphatases
  JOURNAL   EMBO J 21 (17), 4699-4708 (2002)
   PUBMED   12198172
  REMARK    GeneRIF: The central histidine within the DcpS HIT motif is
            critical for decapping activity and defines the HIT motif as a new
            mRNA decapping domain, making DcpS the first member of the HIT
            family of proteins with a defined biological function.
REFERENCE   10 (bases 1 to 5427)
  AUTHORS   Wang,Z. and Kiledjian,M.
  TITLE     Functional link between the mammalian exosome and mRNA decapping
  JOURNAL   Cell 107 (6), 751-762 (2001)
   PUBMED   11747811
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF532613.1, BE382749.1,
            AY040771.1, AP001318.6 and BM998313.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014026.5.
            
            Summary: This gene encodes a member of the histidine triad family
            of pyrophosphatases that removes short mRNA fragments containing
            the 5' mRNA cap structure, which appear in the 3' -> 5' mRNA decay
            pathway, following deadenylation and exosome-mediated turnover.
            This enzyme hydrolyzes the triphosphate linkage of the cap
            structure (7-methylguanosine nucleoside triphosphate) to yield
            7-methylguanosine monophosphate and nucleoside diphosphate. It
            protects the cell from the potentially toxic accumulation of these
            short, capped mRNA fragments, and regulates the activity of other
            cap-binding proteins, which are inhibited by their accumulation. It
            also acts as a transcript-specific modulator of pre-mRNA splicing
            and microRNA turnover. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.506890.1, AY040771.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263579.5/ ENSP00000263579.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               AF532613.1         309-643
            336-679             BE382749.1         306-649
            680-1171            AF532613.1         988-1479
            1172-1175           AY040771.1         1178-1181
            1176-4940           AP001318.6         118026-121790
            4941-5427           BM998313.1         18-504              c
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.2"
     gene            1..5427
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /note="decapping enzyme, scavenger"
                     /db_xref="GeneID:28960"
                     /db_xref="HGNC:HGNC:29812"
                     /db_xref="MIM:610534"
     exon            1..222
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1035
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /EC_number="3.6.1.59"
                     /note="isoform 2 is encoded by transcript variant 2;
                     homolog of C. elegans 7meGMP-directed hydrolase dcs-1;
                     mRNA decapping enzyme; scavenger mRNA-decapping enzyme
                     DcpS; heat shock-like protein 1; 5'-(N(7)-methyl
                     5'-triphosphoguanosine)-[mRNA] diphosphatase; histidine
                     triad protein member 5; hint-related 7meGMP-directed
                     hydrolase; m7GpppX diphosphatase; decapping scavenger
                     enzyme; histidine triad nucleotide-binding protein 5;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="m7GpppX diphosphatase isoform 2"
                     /protein_id="NP_054745.1"
                     /db_xref="CCDS:CCDS8473.1"
                     /db_xref="GeneID:28960"
                     /db_xref="HGNC:HGNC:29812"
                     /db_xref="MIM:610534"
                     /translation="MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPF
                     SGFRLQKVLRESARDKIIFLHGKVNEASGDGDGEDAVVILEKTPFQVEQVAQLLTGSP
                     ELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRN
                     ITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYL
                     IAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHL
                     HVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEA
                     QQS"
     exon            223..397
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     exon            398..543
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     exon            544..657
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     exon            658..768
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     exon            769..5427
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1154..1159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /note="hexamer: AATAAA"
     polyA_site      1175
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /note="major polyA site"
     regulatory      5399..5404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
                     /note="hexamer: AATAAA"
     polyA_site      5427
                     /gene="DCPS"
                     /gene_synonym="ARS; DCS1; HINT-5; HINT5; HSL1; HSPC015"
ORIGIN      
        1 gcacaccgcc tccgcggcag catggcggac gcagctcctc aactaggcaa gaggaagcgc
       61 gaattggacg tggaggaggc ccacgccgcc agcacagagg aaaaggaggc aggagttgga
      121 aatggtacct gtgctcctgt ccgcttaccg ttctccggct tcagactgca gaaggtgctg
      181 agggagtctg cgcgggacaa aatcattttc ctacacggga aggtgaatga ggcctctggg
      241 gatggggatg gagaggatgc cgttgtgatc ctggagaaga cgccatttca ggtggaacag
      301 gtggctcagc tcctgacggg cagccctgag ctccagttgc agttctccaa tgatatctac
      361 agcacctatc acttgttccc tccaagacaa ctgaatgatg taaagacgac cgtggtttac
      421 cctgccacag agaaacacct gcagaagtac ctgcgccagg acctccgcct gatccgagag
      481 acgggagatg actacaggaa tattacttta ccccacctgg agtcccagag cctcagcatc
      541 cagtgggtgt ataacattct cgacaagaag gctgaagcgg accggattgt tttcgagaac
      601 ccagatccct ctgatggttt tgtcctcatc cctgacctca agtggaacca acagcagctc
      661 gatgacttgt acttgatcgc catctgccat cgccggggca tcagatccct acgcgacctt
      721 actccggagc acttgccgct gctcaggaac atcctccacc aggggcagga ggccatcctg
      781 cagcgctacc ggatgaaggg agaccatctg cgagtatacc tgcactacct gccctcctac
      841 taccacctgc atgtgcactt caccgccctg ggcttcgagg cccccggctc aggcgtggag
      901 cgggcccacc tgctggctga ggtgatcgag aacttggagt gtgaccctag gcactaccag
      961 cagcgcacgc tcaccttcgc cctcagggct gacgaccccc tgctcaagct cttgcaggag
     1021 gctcagcaaa gctgaattaa ctcaggcaga agagcacaga tgtgtgggat tgggggagga
     1081 gtggggacaa gattttttat ctccaagtga attttctaaa aatgtatttt ataccggctt
     1141 attcctagta ttgaataaac tagcgggctc actcagtgtg gacagcgtgg cctgggaggc
     1201 agacagatgg tgggggacag tgggtgggta gaacctgtgg gaaggccttg agaatggtgg
     1261 aaagtctcca ggtggtggtt tcaactgaca ggtgggagct gccctggaag ggtgccgagg
     1321 gccttctcca agccccaggg ctcaccgtcc gtggtggctt ccttctcccc actctgtgcc
     1381 tctcctgttt gtacaacttc ttattctgct cctccccatc acccctcctc cttcccctgc
     1441 catccacctc tcctttgagg agttccccaa aaaaccaaac ttggaagctt tcaggaaatt
     1501 atctatacag gtttctgatg gcacttcctc cttccagcag tgaggtcaca cctccctgtg
     1561 agtcagtcaa ccctgctgat aaacttcttg ttgacgagga taggggcttg gtgcctggaa
     1621 atcggcgggt tttaataaga atcagattat gcaaggccga aaagcctgcc ttgctctgtt
     1681 atttggtcag cgagtcccca gccttgagtt tttatctcgg caggaagaca aaacagactc
     1741 acatgaagca gccagaagat caagtgcaaa acagtgaatc agcaagggca aatgatatgg
     1801 ttgcagttga atacaaaagc ccggaggcgg ctcgggttca aggcctatca gaggcagatg
     1861 ttcagaggat cttgagacca ggaagggacc tcaaagcgtc agaggctgat gagcagttag
     1921 gcagccaaca gggctcaagc ggcgcaggga catggcttgt atgggcgggc acacaagtaa
     1981 acacacaggc tctctccagc acacaggacc aaggcatcat gctgccagag gggctggtgt
     2041 ttgccacttt gtcagagtag ggctgtggat tttgtgcctt gcttgggaag ctctcaggga
     2101 tggcaggatg gcaggctcct aagtgacagc aggggggaag cttgaggcac agtgggtggt
     2161 ccttactctc catcccctgc ctgagaacca ggaagcctca ttcagagatt agccccttta
     2221 ctgcaggtgg tagcccaggt gcagccaacc tgcttcaggg tctcagggcc tgctgtggac
     2281 ccccatggtc ctgggagaga ctccttagaa acagcagaat cacgaacatg gtgaaaccca
     2341 tctctactaa aaaacagaaa aattagccag gtgtggtggt gggctcctat aatcccacct
     2401 acttgggagg ctgaagcagg agaatccctt gaacccggga ggcggaggtt gccgtgagcc
     2461 accgcacttt ggcctgggtg acagagcaag actccctctc agaaaaaaaa attaaaaaaa
     2521 tagaaacagc cggggagtgg agggtgtggt cagaaagcct cagggacctg cttcagcctt
     2581 cagggagttc agatgaggca caatgaagcc actggcctcc actcagccat tcttccctgc
     2641 agctcttttt tttttttttt tttttgagac aatctcgctc catcacccag gctggagtgc
     2701 agtggcacaa tctcgactca ctgcaacctc tgcctcctgg gttcaagtga ttctcctgcc
     2761 tcagcctcct gagtagctgg gactacaggg gtgcgccccc atgcccagct aatttttgta
     2821 tttttagtag agacggggtt tcaccatgtt ggccaggctg gtctccaact cctggcctca
     2881 ggtgatccac ccatctcggc ctcccaaagt gctgggattc caggcgtgag ccaccgcgcc
     2941 cagcccctgc aatacgtcaa cagtccctag attcaggcaa catggagttg cagccaagga
     3001 actttgaggt agtgaccaat gcagggaggt ctgacagaga atcagcagga tccatttcat
     3061 ggccaccaca catctgactg actccttctt gggctaagca aatacttttg tgggcagtga
     3121 cactggcagc tgctggggtc ttggcaggag gttggatgga ggtcttctcc cagagcagtg
     3181 tccagcttgc aggaatgctc cctgcgtctc agttgtgccc attccaggtc caggggaaag
     3241 aagtgacccg cttcccctct gaaagcagat gtggtcccaa caagcaaaca cggtctccat
     3301 ctccagcacc acaactgcca cggagcagag ggaggcctcc ctgtggagca gcccttccct
     3361 gtggcctcag cccatgccct cctgcccagc ccccagtggt gctgctggag ggtctgactc
     3421 cacgggctcc cgctgacctt gccgttcctt cccctcatct cgtagcgcct ggcccctgga
     3481 ggaaggtggg taggaatttg acaccggata aataaatgtt tgaggggcaa gagagatggg
     3541 acagagttca cccaacagga cagacgaggc tggcagtcac agcagagggc aggcactcag
     3601 gtctggagag gcagggcatg gagacaggga cccacactct ggaaggtttc tcgactcccc
     3661 cgaggggctg gccagttctg tcttccttct gcccactctg tggggaggag cctctctggg
     3721 atagcgtccc ctcactcctc tcccagcctc gtttatcttc accacagtac agaagggggc
     3781 ccctctccag gcctcaccac ttaacttttc ttgtacatgg aaagacaagc ctctgctaga
     3841 ggcctggcaa gggttcccct tcagagccag ccagtgctct gctgctgtaa tctccaggga
     3901 ccctgccggc cacggccccc atgcagctcc ctccctcaat ccaaccctct ttgggcttat
     3961 caccctcaag gtctagagtg caccagggtt ggctgctctg ccaagtcctg ttgagactcc
     4021 aaaagcatag tctgtcttta ttagggtgac cttcatatca gactcccagg taactcaaga
     4081 tgatagcttt gcaatatagt gcctcctttt attcctattt gtgagtttct ccgctttcct
     4141 cactagactg agagggtctt gagggtagga gctttgtttt agttatctct ctatccctgg
     4201 cccctaagac actacctggc tcagcaaacc aacctttgag acaaagcaac attataacac
     4261 tacattcata tagcagatca tttcatgacc cacattctcc ttccttttta cgaagaaatg
     4321 ggctcaggat gtcttgtcca gcattatgga gtgaacgtca gctccaggaa gcagagactt
     4381 ctggcccttt gttcaccatt tccccagaac ctagggtggt gactcaccta taagtgctca
     4441 aaaaacatgt ggcgaatgga ggaccagagc taggctctga atgaggcctc ctggatctca
     4501 cgcaggggat ggagagtaag gaccagcccc tctacctcat gctttcttcc tgctatctcg
     4561 taagagccca acatccttct gtcctgagca agccacaagc tgcagccctg ccttgacaaa
     4621 atggcaaatg ccagcccctc tggcagcatg acaccttggt cctgtgtgct gcagagtgcc
     4681 tcatgtgttg aactgtgtgc cctcagaagc atgccatgcc cccgctctgc tggagcagtg
     4741 gcagatgtca cccttcctag ataaatcctg gacggctcca cgactggctt ttagccctca
     4801 acacttggcc tacattcttt ctggcttggt tggagaagga aagaagcagt tggaggcatg
     4861 actgctattg ttccttggct tctctccatt tgtctcaaac tttcctaata gagcaaaatt
     4921 gctgagtggt ctctatttac caacatgaaa cagaaaaata ccatctcagg ctccatattc
     4981 catgtccctg ttgaatacag atccccgaac tgagaaataa gaaacaacta catacgaagt
     5041 aatacttgaa aatgtttctc ttgtaatact tccatatcca aagcatttga atatatttat
     5101 tttattgatg taggtctgcc aactcttcct tctcaggaag gactgttttt attccaaaca
     5161 tgtatatata aggttaaaat gtatttttta aaataagata attattgata gttgttttta
     5221 aaatagcctt gcttagcaaa ataaatgttg gcaaccctaa gtttgttcta ttataatatt
     5281 attgaaatta cattgaattc tgaaatccat gtcaacttga agttaccaag ttttaaccac
     5341 tgttagaaaa taagcaggaa tgaaatctgt ttcaagcgtt tcaatttcaa tacaatttaa
     5401 taaacaggct ttgagcatct accccca
//
