LOCUS       NM_152624              10110 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens decapping mRNA 2 (DCP2), transcript variant 1, mRNA.
ACCESSION   NM_152624
VERSION     NM_152624.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Mei,Y., Lv,Q., Tan,Z., Zhang,Z., Ji,Y., Chen,S. and Shen,X.
  TITLE     Decapping enzyme 2 is a novel immune-related biomarker that
            predicts poor prognosis in glioma
  JOURNAL   Biotechnol Genet Eng Rev 40 (4), 4262-4283 (2024)
   PUBMED   37191010
  REMARK    GeneRIF: Decapping enzyme 2 is a novel immune-related biomarker
            that predicts poor prognosis in glioma.
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Liu,Q., Yi,D., Ding,J., Mao,Y., Wang,S., Ma,L., Li,Q., Wang,J.,
            Zhang,Y., Zhao,J., Guo,S., Liu,Z., Guo,F., Zhao,D., Liang,C.,
            Li,X., Peng,X. and Cen,S.
  TITLE     MOV10 recruits DCP2 to decap human LINE-1 RNA by forming large
            cytoplasmic granules with phase separation properties
  JOURNAL   EMBO Rep 24 (9), e56512 (2023)
   PUBMED   37437058
  REMARK    GeneRIF: MOV10 recruits DCP2 to decap human LINE-1 RNA by forming
            large cytoplasmic granules with phase separation properties.
REFERENCE   3  (bases 1 to 10110)
  AUTHORS   Maciej,V.D., Mateva,N., Schwarz,J., Dittmers,T., Mallick,M.,
            Urlaub,H. and Chakrabarti,S.
  TITLE     Intrinsically disordered regions of tristetraprolin and DCP2
            directly interact to mediate decay of ARE-mRNA
  JOURNAL   Nucleic Acids Res 50 (18), 10665-10679 (2022)
   PUBMED   36130271
  REMARK    GeneRIF: Intrinsically disordered regions of tristetraprolin and
            DCP2 directly interact to mediate decay of ARE-mRNA.
REFERENCE   4  (bases 1 to 10110)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 10110)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 10110)
  AUTHORS   van Dijk,E., Cougot,N., Meyer,S., Babajko,S., Wahle,E. and
            Seraphin,B.
  TITLE     Human Dcp2: a catalytically active mRNA decapping enzyme located in
            specific cytoplasmic structures
  JOURNAL   EMBO J 21 (24), 6915-6924 (2002)
   PUBMED   12486012
  REMARK    GeneRIF: Human Dcp2 is a catalytically active mRNA decapping enzyme
            that localizes to the cytoplasm
REFERENCE   7  (bases 1 to 10110)
  AUTHORS   Ingelfinger,D., Arndt-Jovin,D.J., Luhrmann,R. and Achsel,T.
  TITLE     The human LSm1-7 proteins colocalize with the mRNA-degrading
            enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci
  JOURNAL   RNA 8 (12), 1489-1501 (2002)
   PUBMED   12515382
  REMARK    GeneRIF: LSm1-7 proteins colocalize with DCP1,DCP2 and Xrn1 in
            cytoplasmic foci
REFERENCE   8  (bases 1 to 10110)
  AUTHORS   Lykke-Andersen,J.
  TITLE     Identification of a human decapping complex associated with hUpf
            proteins in nonsense-mediated decay
  JOURNAL   Mol Cell Biol 22 (23), 8114-8121 (2002)
   PUBMED   12417715
  REMARK    GeneRIF: These data suggest that a human decapping complex
            containing decapping enzymes hDcp1a and hDcp2 may be recruited to
            mRNAs containing premature termination codons by the hUpf proteins.
REFERENCE   9  (bases 1 to 10110)
  AUTHORS   Wang,Z., Jiao,X., Carr-Schmid,A. and Kiledjian,M.
  TITLE     The hDcp2 protein is a mammalian mRNA decapping enzyme
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 12663-12668 (2002)
   PUBMED   12218187
  REMARK    GeneRIF: an mRNA decapping enzyme demonstrated to contain intrinsic
            decapping activity
REFERENCE   10 (bases 1 to 10110)
  AUTHORS   Ueno,K., Kumagai,T., Kijima,T., Kishimoto,T. and Hosoe,S.
  TITLE     Cloning and tissue expression of cDNAs from chromosome 5q21-22
            which is frequently deleted in advanced lung cancer
  JOURNAL   Hum Genet 102 (1), 63-68 (1998)
   PUBMED   9490301
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY146650.1, AK090564.1,
            AY135173.1, BC045596.1, DB146225.1, BX648838.1, BX537716.1,
            AL135390.1, AC008536.7, CF994730.1 and AA837315.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152624.5.
            
            Summary: The protein encoded by this gene is a key component of an
            mRNA-decapping complex required for degradation of mRNAs, both in
            normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It
            removes the 7-methyl guanine cap structure from mRNA, prior to its
            degradation from the 5' end. Alternatively spliced transcript
            variants encoding different isoforms have been noted for this
            gene.[provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.181407.1,
                                           SRR1803617.205736.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389063.3/ ENSP00000373715.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-477               AY146650.1         63-539
            478-1930            AK090564.1         481-1933
            1931-2486           AY135173.1         1993-2548
            2487-4772           BC045596.1         1872-4157
            4773-5139           DB146225.1         167-533
            5140-7115           BX648838.1         4640-6615
            7116-7681           BX537716.1         3292-3857
            7682-7885           BX648838.1         7182-7385
            7886-8412           AL135390.1         91-617
            8413-8885           BX537716.1         4589-5061
            8886-9307           AC008536.7         161278-161699
            9308-9874           CF994730.1         75-641
            9875-10110          AA837315.1         34-269              c
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q22.2"
     gene            1..10110
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /note="decapping mRNA 2"
                     /db_xref="GeneID:167227"
                     /db_xref="HGNC:HGNC:24452"
                     /db_xref="MIM:609844"
     exon            1..189
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..1399
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /EC_number="3.6.1.62"
                     /note="isoform 1 is encoded by transcript variant 1; nudix
                     (nucleoside diphosphate linked moiety X)-type motif 20;
                     mRNA-decapping enzyme 2; hDpc; m7GpppN-mRNA hydrolase;
                     DCP2 decapping enzyme homolog"
                     /codon_start=1
                     /product="m7GpppN-mRNA hydrolase isoform 1"
                     /protein_id="NP_689837.2"
                     /db_xref="CCDS:CCDS34210.1"
                     /db_xref="GeneID:167227"
                     /db_xref="HGNC:HGNC:24452"
                     /db_xref="MIM:609844"
                     /translation="METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHW
                     FYLDFYMQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGA
                     IILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETGFDIKDYICKD
                     DYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRNDMTPKSKL
                     GLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHS
                     QQLFPDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKNQSMRGNGRKQYQDSPNQKK
                     RTNGLQPAKQQNSLMKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAEGQPVACN
                     GHCKFPFSSRAFLSFKFDHNAIMKILDL"
     exon            190..341
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            342..469
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            470..568
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            569..721
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            722..834
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            835..942
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1078
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1183
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1235
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..10110
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8846..8851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /note="hexamer: AATAAA"
     polyA_site      8885
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /note="major polyA site"
     regulatory      8956..8961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /note="hexamer: AATAAA"
     polyA_site      8981
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
     regulatory      10086..10091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
                     /note="hexamer: AATAAA"
     polyA_site      10110
                     /gene="DCP2"
                     /gene_synonym="NUDT20"
ORIGIN      
        1 ctcgtctccg ttggagtcgt ctctgccgcg gcttcctcgg ctgccagctc tccggcgagc
       61 cggagtccta gtgccgtacc gtcagtcccc ggccgcgcgg agccgggatg cactgttcct
      121 gctgtgggtc ctcatcatgg agaccaaacg ggtggagatt cccggcagcg tcctggacga
      181 tctctgcagc cgatttattt tgcatattcc cagcgaggaa agagacaatg caatccgagt
      241 gtgttttcag attgaacttg cccattggtt ttacttggat ttctacatgc agaacacacc
      301 aggattacct cagtgtggga taagagactt tgctaaagct gtcttcagtc attgtccgtt
      361 tttgctgcct caaggtgaag atgtggaaaa agttttggat gaatggaagg aatataaaat
      421 gggagtacca acatatggtg caattattct tgatgagaca cttgaaaatg tactactagt
      481 tcaggggtac ctagcaaaat caggctgggg atttccaaaa ggaaaagtaa ataaagaaga
      541 agctcctcat gattgtgctg ctagagaggt ctttgaagaa actggttttg atatcaaaga
      601 ctatatttgt aaggatgatt acattgaact tcgaatcaat gaccagcttg ctcgtttgta
      661 catcattcca ggaattccaa aagacacaaa atttaaccca aaaactagaa gagaaattcg
      721 gaacattgag tggttctcta ttgagaaatt gccttgtcat agaaatgata tgacccccaa
      781 atccaaactt ggtttggcac ctaacaaatt ttttatggcc attcccttta tcagaccatt
      841 aagggactgg ctttctcgaa gatttggcga ttcctcagac agtgacaatg gattttcctc
      901 aactggtagc acgccggcta aacccactgt ggaaaaattg agtcgaacca aattccgcca
      961 cagtcagcag ttatttcctg acggttctcc tggtgaccag tgggtaaagc acaggcaacc
     1021 actgcagcaa aagccatata ataatcattc tgaaatgtct gaccttttaa aaggaaagaa
     1081 tcaaagtatg aggggaaatg gcagaaaaca gtatcaagat tcacctaatc aaaagaaaag
     1141 aacaaatggg cttcagccag caaagcagca gaattctttg atgaagtgtg aaaagaaact
     1201 tcatccacgg aaacttcagg ataattttga aacagatgct gtatatgact tgcctagctc
     1261 cagtgaagac cagttgctag aacatgctga gggacagccc gtggcatgta atggacattg
     1321 caagttcccc ttttcatcca gagccttttt gagtttcaag tttgaccata atgctataat
     1381 gaaaatcttg gacctttgat agcagcacat gtattgtaaa tgtcccagga tcagagacct
     1441 gttgaatttg agtgggtgtc tcctcaagcc ttacctttct caggtgtttt aaagaaatgc
     1501 agggaggcaa tgtttctgaa gacattttct gtttataaga gagtagaaag aaacacgagt
     1561 ttgcactgta aatgcagtta taacctttta tacagattta ccttttcagt gttcagtaca
     1621 agtttaagtt gctttctttg agggcattta ttctgtgtga ctgtgggttt tattttgtat
     1681 tctggttaag aaaataatgt attgagttac tgtcaagtag ccaagttaat gggaatgctc
     1741 catctacctg ttacagtgat tgcaataata gtatattgga gtttttcaaa gaaacttaaa
     1801 gtaatgccca attattaaat gaacacaaga gctgagatgt cacattcgtc agcattacag
     1861 gtgatctatt ttggtggcat tttgtacttc tctctgcttc taaccactga ggctctctaa
     1921 tcttcctctg gagttttagt gaaaggattt attgagcagc ttctggaata taatgtgcat
     1981 gtccaaaatg aactcagcgc ttcaaaagga caaagtctgt agcctggagg ggcttgagtg
     2041 gatgggagct gatgctgtga ttttgagctg tggttacatg cagtcagtaa acctgtgaga
     2101 ctgctggagg aaatgtagca gacagcatgg aggctgggac ccagcagcta ctttgggtca
     2161 tgtctttact gtcctgcctc caacccttta gtctcgtaga cttttgttct tgtggaaatt
     2221 tcttctgtat tccagttgtg taaatatgta tggaaaactg atattactag gttttacgtt
     2281 gcatctccag tattgatctt tggaaactga tgttacatta ggttccaatt cgcaatagta
     2341 gcagagactg acatgctttt attgacctgc taagcccctg gatgatggag cgagaaggga
     2401 aggggtatgc agtttaccca agtccaaata aagcatttca ctaggttaca cgttatgtat
     2461 atgtgttaca taacagtaac atacataact cactcctagg aatgtatttt gcttcaggat
     2521 ttttaaattc aaataaggat ggcagtgccc attttaagga ggatttcagc tataactgag
     2581 gacaatcacc agggcgttaa gggggcttta gtgttttggc tgcatctgac ctcattgaag
     2641 gaatttcttg ggagtcgggg gtgtttgagt atatgtgtgt gtgtgtaact atcatgtggg
     2701 ttttaaaaat ccttttttac agtggtatcc acaaaatact tctctgctta taaatgttat
     2761 tgtagaaatc tttattctgt gtaggtagcc tatttaattg ggttccactg aacctcatgg
     2821 gacttttaaa agaagggcta tttaaggaat tcacttggta gtactttgct ttagctttta
     2881 aatggcttga ttttaaagga gaaattctat ttattaatga aagtgtcacc cttttggtgc
     2941 taagcaggag aattcacaat taaagcagtg tgtatagaat tatgatattc agaatcctgc
     3001 atcttttaat gtgacaaatt tttgaatcct gtgacttttg ctttttaatg cattatatga
     3061 ttgtgtttgg aagcttttac tgttcttttc agaaatacgg ctgctttgct tgggtctctt
     3121 cgagccatat ttatagttgg tcccagcatt ccagacttct ggtaaattgt taaaggagtg
     3181 aaaatgtagt ttgcctggca gatgaataat tttcactcat ttattgtctt ctggaaacca
     3241 tttaactttt actgtttttt atttttttgg tatgtgtttt tttttttctt tttaagtgca
     3301 tactttggcc agtcttcctg gtgcctgtag agttggattt ttcagctctc aatactgaag
     3361 caaaagagat tatagttacc agaagtatgg cctctaatgg tgttgagcct tttcaagaac
     3421 caacaaatta tctttgtgat tacagtagtt taactatggt taaatgttac tgctgttgcg
     3481 ctgctttatt taatgcactg tggaacaaaa tataagggta ctgttggggt gtgattatga
     3541 taacttcagg cttttagctc tcctcaaagt ttgaggttaa aaaaatgtta aaagatgtga
     3601 gttaaagttt atgtgtgtga tgtgacttaa ctatgcagaa aatatttaag ttggatgtac
     3661 ttgttttagg tacatctact atacttattc tctcaaacct gggtaatagt ttctcagtgt
     3721 tcaggttacc ttgagaaagg ccatgcttag cgaactggat ttctcctttg agaaatttta
     3781 gtatggtttg aggaaatcaa gtgataaagg agtatgctga atatgcaatc atttacttct
     3841 actgagtgct gcattttaag atgctagatg agaagagtag ttggttttgc ttcattatgt
     3901 tttatgtctt tgagagagat acaggtcttg gaattcactt tttaaatgtt acgttccaac
     3961 cttatatgtt ccaaggtgca gtgcactgtg aatcttttat ttttaaaaaa atttgtaggt
     4021 gcttttatgt ataactttaa tgatttataa gatgctaaat gaattgtttt tgtacctaac
     4081 cattgtaatc caaaagataa gggtgtggtt tacatgcttt tcaaaaattt ttgttaagaa
     4141 gtagcaaatg aatggtttag gatttcaaat agtgataccc tcctgagaca tgagcatttg
     4201 gagaggcctg agaacctaat agaactcata tagtgtgagt gaaatttgat taaagatgtt
     4261 ggaaaatgaa gttggatttt tgtattcatg tataaaggaa agtctcccag tgctgtcaaa
     4321 ggaattgtgg ttactacttg ctgttctgag ctttgaatat cgttattttt ctattaaata
     4381 tcaattagta agtatagcta atgaagacat ataatgaaag taattttatg ctgtaagatt
     4441 gaattggtac ttttccttat taaggcttta gtaatttgta tgtaatgtca aataaaagga
     4501 attcaaatct gagtaaataa cagcctccta ctataggaat tagattcttc tataaaagtc
     4561 accatattgt atcctataga aaagttaagg tctgagtgca tgtgtgcatt aaaaacattt
     4621 cactacctat tctctcatgg acttctcatc ctttcttttt tcatctccaa cagttaaatg
     4681 gtaaggagag caaagttaat tataaaaatt agacattttg ccaagttagt tttcttacca
     4741 caataccctc ttggcttttt tttttttttt tttgagatgg agtttcactc ctgttgccca
     4801 ggctggagtg caatggtgca atttcggctc accgcaacct acgcctcccg ggttcaagtg
     4861 attctcctgc ctcagcctcc caagtagctg ggattacagg catgcgccac cacgccctgc
     4921 taattctgtc tttttagtag agacggggtt tctccatgtt ggtcagggtg gtctctcttg
     4981 gctttttatc tttatttttg taccagtgga ccttcctgct ccctgagact gttttgattg
     5041 acatcttttg tgtttctata ttttccgagg cagtattttc tttgtatgtt aatcatagtt
     5101 atagtaaagt cagactcatt aacttttctt cctagtaatt ttgacttgga ttattttcta
     5161 gcctggggga tggggttcat atgtgttgtt gtaactttag attactaaac aaaacaaact
     5221 gtttttttgt ttgaaggtat cctttacata cctgtctgta ggtaagcaag tgttagtaaa
     5281 aacaattttg tcaaacagta ctcctgacag cttggtaaag tctaggtaga gtttctgatt
     5341 ttgtattatg tcctaagact tgaaagtgca tttgtctagt tgccaaaagt tctaaaaatg
     5401 agatggtggg ctttctcagc atctttttgc ttcctagaat tgagttgctc ttgaagtctg
     5461 tactcctgtg tcagaaagat ggcagactgc aaatgggcag tacaggcttt gccccatgct
     5521 ttcaaagtag gattctggat tgcagtttca aaaaaatatg atagattatt gttatagaat
     5581 caaagtgtcc tgaagggaga agaatagcta tagttgctgg gcaaagagtg taagaaagaa
     5641 actttagagc atttatcaat acttgagtgc tttattatgt tccaggctag aaatgtgatc
     5701 attgcatggc ctaagtgtag tcattcatat atatatgaat atctatatct atatctatac
     5761 atatatatgt atcgccatta tcgagtgttt tctagccctg gataaagtta atttttttga
     5821 agtttgattg ccaagcaatc cagtgaatct aaagtaattt ttctcacttt aaagaccagt
     5881 gatgaggagt ctggtccttc ctgtactgat actgtagaac tccagtcaaa cttcagtgca
     5941 ctgctttctt acaggaagac aactcagaac acacagccat aatctgcttt ggtgtcagtc
     6001 acatcacagt ggttatacat ttgtcctgaa tggtgattca ttcctttcca tctaacttcc
     6061 ttgtgacaaa tagcatttac tattgaaata gtcttaaaat agggaaaaga gtgaaagtta
     6121 ctcttatgca tgggagtggg cttaaagggt tgacttccac cagctgagta caaacaagtg
     6181 taaagaaaaa ctatgataaa gtatggaggt cttgagaatt gttcataatg aaatctttaa
     6241 tctgggttat cagagtaatg aaatcaccaa taaaaacatt ccatttatct tggatcttct
     6301 gtggttagca gaggagtatg gtctagctat gttagcagct tgaaggcaca acgtaaaagg
     6361 aatgagcttt cacaaggtgc ttacgttagc tgggtagtat tggtcaaagg tactctgaag
     6421 agtgaatgca gaaatcagtt ggctgtgttt gtaatcttgt cccacatgga ccctaacctt
     6481 tagtagactt cagtctttta gtccagtaga agacaatggc cctgaatatg tgttttctgc
     6541 tttttggtgg aaggtttcct gcaggtggtt gggtggtttt tgggttattg gctactgttc
     6601 gttcagtgcc atttgaagac ctgttgtttc ttaatccttt ttcttggtgt gactggccag
     6661 gaaaaagtcc attgagtcct ttttttctcc ctggtgtctt accccttctg tagaataatc
     6721 catattttaa aatttgtttc acatctttat agcaaacctg ccacagactt gcaagcagtg
     6781 attttatctt tccttcctga gaaggtgatc tcacttttga tgtttgggtc acctgtggac
     6841 acctgtgctc ttttgagtct caaggcttat agctttcaca ttggcttggg aacaacagac
     6901 ttgtgtgtgg ttctagagtg aaatgggcag tgttctgctg gtcctcagtc tttgagaggt
     6961 cttaggcaac atccttagta tagtggaatt tctgaggtca aatttaacat ttaatatatt
     7021 ctccttttag ttcattgaat ctgaggttct aaatctgtag cttcattttg agcacaagtc
     7081 tgcattgtca ttttttaaga gattggcagg catatccttt tgtgatactt aggaaaagac
     7141 tatggatggc agcaaatgaa agtaaaggtt ctcaactgtg aatgaaattt agaaagataa
     7201 ttcttcagca gttggttgat tagatgggtt agtgcttttg gaatatataa cagtagactt
     7261 gtattcttta tgcactatac atatgatacg cactgacaga aaagcacaga agacatactg
     7321 ctaagagttt ctttgtgcat ttcactactg ttctagggga ctgctgtctc tttggttcct
     7381 tttaggtttg tgttaggatg ttgcacaagt aatcatttaa tgttaaatgc aagtgtccaa
     7441 agaaatgagt cttaaagttc tttggtcata tttcacttta gagttaggac aggagacagc
     7501 taacctgact tccattttac aaatattaga aactttttct tcagtaacaa aaacagggaa
     7561 ggacaataaa accatgaact attttgcttt atgtctgttt ttaaaaaata attgttttga
     7621 agtttgctta ttagcttaat accttatagt acttttaaaa aatcatcctt ggtaattatt
     7681 gacttatcct agatccatgt acaattcaga cttttatctg ttagctttaa taaaaattta
     7741 aaaccatctc atttcagaat aaaataaacg gaattacagt tcatgcatta gcatttttaa
     7801 gtgaagttct cttaataaga ttgccttttg tctaaccgaa atagggcata cagattagtt
     7861 tggatgggaa agtggtaaga cttgactttt taatactgtg cctatttcca aaagaagact
     7921 tgatgtggct ttttacatta tgctttaaag atgttaatgg aggatacata gtcttaaaac
     7981 aatttgtttg ggagtaagtt ttagtcggaa aaaggtgaag atatttatgc tgagttatgt
     8041 taagcaaatt attttgagac cttttgctgg tagacaaaaa ctgaggaaat gtttcactaa
     8101 atatattatg gtcaatgaaa aaaccagact ttagagtaaa tgtgataacc aagtagattt
     8161 gtgaacttta tttctttagt aattattttt atcttatgtg aaatgattca acctttaatt
     8221 tttgcctaga ttatagcaat gttttgtttt accttatagg gcattttaat gcttggagaa
     8281 agactataag tgaatctact tcaagggatt ctgttcatgg tggtatacag ttcttggtgc
     8341 tcttttggaa attgtcaaac atttactgat agcagaaaag gaaccccaaa acacctgtag
     8401 tgttcacctt gttataagaa cagaaacatt tggaacaggt ttcattctgt ttctagattt
     8461 atgttgttgt agttgaacag caactgtttt tttccctcag tgttaacgaa aggataaaga
     8521 ctacctgtat tgttgggtat gactatcaaa ggatttccgg tgattcatgt ttgtgaattg
     8581 gagtttaaat gtttgcagga ttactgtact tccaaagacc cactagaatg tcagctgtac
     8641 tctgtactct ccactgagaa aatgaacaaa atcctatgtc ttaacagtta tgctctaact
     8701 ttttgaaagc tttttgatgt aaataatagg ttagaaacta aaagcctttt aatcttacaa
     8761 aatacaattt gaacgatgga aggttgttca gatactggct ttctgtaaaa acatcattgt
     8821 gttagattgt ttgtaatgta ctatcaataa aattggctgc ttgggcggtt ttagttacca
     8881 ccttagcctg gttttctgct actgttattt atcatcgtag gttctgagtt gctcattgtg
     8941 gttgctcttt ggaccaataa aaatggcaag tatgagatga acatgataaa agtatgttta
     9001 tataacagga agaagctggg cgcggtgctc atacctgtaa tcccagcact ttgggaggct
     9061 gagttgggtg gatcacgagg tcaggagttc gagaccagcc tgaccaacat ggtgaaacta
     9121 aaaattcaaa aattagctgg gcgtggtggt gcgtgtctgt agtcccagct acttgggagg
     9181 ctgaggcagg agaatctctt gaccccggga ggcagaggtc gcagtgagct gagatcacac
     9241 cactgcactc cagcctgggt gaacagagca agactctgtc tggaaaaaac aaaaaacaac
     9301 caaaaaaaaa aaaaaaaaac ccccaggaag aaatattgtc gagagtctct gcaaaaatga
     9361 aaatacctca aacaattaag tttgttgctt taaagcaaga ttatagtgta gggcttgaga
     9421 tgtgaattca ttagatattt aaaatccagc aattcttaag tttgtgctag aatcaggttt
     9481 tgcaatagga atacttaact ttggatctca agggggaaag aaatacattc ttttccatta
     9541 gagacctcag ctatatgata gtaactttta ttttaaattc tcaaaaggag agtgactaag
     9601 gtgttatact tactgtcaaa gttactttgc ttccatagtg aaaacttgag aacattgtaa
     9661 tctttctcta acagatgtgt tgcaaagctg cttgccatct tgttgtccta tatgacttcc
     9721 ttgcatttcc agtccttgat tacattccat tttaatgatc tttctttaag aggggaaaag
     9781 actctcttca atagataact tcctatttat gccaaatttt aaaaagccaa ctaaaaatgg
     9841 gctataaatg agggttcttt ggccattttg atctaataat agtcttactt ctagacccct
     9901 gctttgacta gggctcttga tttcatgtaa gcatcatgga tttgatattt gcctgcatgt
     9961 atatcacagt tctacagaag gatagcctta ccctttaaat aacacagtta aggtacactg
    10021 tggaattaga aatatttatt cagaatataa gaatgtttgt aaaatattat aaatgtctct
    10081 gtataaataa atggagtttt taaaaaacaa
//
