LOCUS       NM_001040260            4075 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens doublecortin like kinase 2 (DCLK2), transcript variant
            1, mRNA.
ACCESSION   NM_001040260
VERSION     NM_001040260.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Hu,L., Zhang,Y., Guo,L., Zhong,H., Xie,L., Zhou,J., Liao,C.,
            Yao,H., Fang,J., Liu,H., Zhang,C., Zhang,H., Zhu,X., Luo,M., von
            Kriegsheim,A., Li,B., Luo,W., Zhang,X., Chen,X., Mendell,J.T.,
            Xu,L., Kapur,P., Baldwin,A.S., Brugarolas,J. and Zhang,Q.
  TITLE     Kinome-wide siRNA screen identifies a DCLK2-TBK1 oncogenic
            signaling axis in clear cell renal cell carcinoma
  JOURNAL   Mol Cell 84 (4), 776-790 (2024)
   PUBMED   38211588
  REMARK    GeneRIF: Kinome-wide siRNA screen identifies a DCLK2-TBK1 oncogenic
            signaling axis in clear cell renal cell carcinoma.
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 4075)
  AUTHORS   Song,J., Merrill,R.A., Usachev,A.Y. and Strack,S.
  TITLE     The X-linked intellectual disability gene product and E3 ubiquitin
            ligase KLHL15 degrades doublecortin proteins to constrain neuronal
            dendritogenesis
  JOURNAL   J Biol Chem 296, 100082 (2021)
   PUBMED   33199366
REFERENCE   4  (bases 1 to 4075)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 4075)
  AUTHORS   Nawabi,H., Belin,S., Cartoni,R., Williams,P.R., Wang,C.,
            Latremoliere,A., Wang,X., Zhu,J., Taub,D.G., Fu,X., Yu,B., Gu,X.,
            Woolf,C.J., Liu,J.S., Gabel,C.V., Steen,J.A. and He,Z.
  TITLE     Doublecortin-Like Kinases Promote Neuronal Survival and Induce
            Growth Cone Reformation via Distinct Mechanisms
  JOURNAL   Neuron 88 (4), 704-719 (2015)
   PUBMED   26526391
REFERENCE   6  (bases 1 to 4075)
  AUTHORS   Kerjan,G., Koizumi,H., Han,E.B., Dube,C.M., Djakovic,S.N.,
            Patrick,G.N., Baram,T.Z., Heinemann,S.F. and Gleeson,J.G.
  TITLE     Mice lacking doublecortin and doublecortin-like kinase 2 display
            altered hippocampal neuronal maturation and spontaneous seizures
  JOURNAL   Proc Natl Acad Sci U S A 106 (16), 6766-6771 (2009)
   PUBMED   19342486
REFERENCE   7  (bases 1 to 4075)
  AUTHORS   Gorelik,A., Sapir,T. and Reiner,O.
  TITLE     Gene trapping: an antibody-dependent approach for verifying
            integration in your favorite gene
  JOURNAL   Cell Mol Biol Lett 13 (4), 614-620 (2008)
   PUBMED   18618084
REFERENCE   8  (bases 1 to 4075)
  AUTHORS   Tuy,F.P., Saillour,Y., Kappeler,C., Chelly,J. and Francis,F.
  TITLE     Alternative transcripts of Dclk1 and Dclk2 and their expression in
            doublecortin knockout mice
  JOURNAL   Dev Neurosci 30 (1-3), 171-186 (2008)
   PUBMED   18075264
REFERENCE   9  (bases 1 to 4075)
  AUTHORS   Reiner,O., Coquelle,F.M., Peter,B., Levy,T., Kaplan,A., Sapir,T.,
            Orr,I., Barkai,N., Eichele,G. and Bergmann,S.
  TITLE     The evolving doublecortin (DCX) superfamily
  JOURNAL   BMC Genomics 7, 188 (2006)
   PUBMED   16869982
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 4075)
  AUTHORS   Edelman,A.M., Kim,W.Y., Higgins,D., Goldstein,E.G., Oberdoerster,M.
            and Sigurdson,W.
  TITLE     Doublecortin kinase-2, a novel doublecortin-related protein kinase
            associated with terminal segments of axons and dendrites
  JOURNAL   J Biol Chem 280 (9), 8531-8543 (2005)
   PUBMED   15611072
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB209181.1, DA122884.1,
            AC093748.3 and AI419589.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001040260.3.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmodulin-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. Mouse
            studies show that the DCX gene, another family member, and this
            gene share function in the establishment of hippocampal
            organization and that their absence results in a severe epileptic
            phenotype and lethality, as described in human patients with
            lissencephaly. Multiple alternatively spliced transcript variants
            have been identified. [provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (1) is known as exon-5-lacking
            transcript (PMIDs:15611072 and 18075264). It lacks an in-frame
            coding exon in the 5' region, as compared to variant 2. The
            resulting isoform (a) lacks an internal segment, as compared to
            isoform b.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB209181.1, SRR7346977.2549475.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296550.12/ ENSP00000296550.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-418               AB209181.1         172-589
            419-870             DA122884.1         59-510
            871-2824            AB209181.1         1042-2995
            2825-3981           AC093748.3         77948-79104
            3982-4075           AI419589.1         29-122              c
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.23-q31.3"
     gene            1..4075
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /note="doublecortin like kinase 2"
                     /db_xref="GeneID:166614"
                     /db_xref="HGNC:HGNC:19002"
                     /db_xref="MIM:613166"
     exon            1..1004
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             584..2884
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1;
                     doublecortin and CaM kinase-like 2;
                     serine/threonine-protein kinase DCLK2; CaMK-like CREB
                     regulatory kinase 2; doublecortin-like and CAM kinase-like
                     2; doublecortin domain-containing protein 3B"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK2 isoform a"
                     /protein_id="NP_001035350.2"
                     /db_xref="CCDS:CCDS34076.1"
                     /db_xref="GeneID:166614"
                     /db_xref="HGNC:HGNC:19002"
                     /db_xref="MIM:613166"
                     /translation="MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGL
                     IPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISSDRFRSFDAL
                     LIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKN
                     INPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNK
                     KTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRY
                     AQDDFVLDHSECRVLKSSYSRSSAVKYSGSKSPGPSRRSKSPASVNGTPSSQLSTPKS
                     TKSSSSSPTSPGSFRGLKQISAHGRSSSNVNGGPELDRCISPEGVNGNRCSESSTLLE
                     KYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHP
                     NIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHG
                     LSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAET
                     GYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSA
                     KELISQMLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNS
                     TTTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISPVPPSVEEIPVPGEAVPAPTP
                     PESPTPHPPPAAPGGERAGTWRRHRD"
     exon            1005..1339
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1442
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1544
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1639
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1715
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1824
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1882
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2002
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2149
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2283
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2361
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..2458
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..2539
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2656
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..4075
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4050..4055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /note="hexamer: AATAAA"
     polyA_site      4075
                     /gene="DCLK2"
                     /gene_synonym="CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2;
                     DCDC3; DCDC3B; DCK2"
                     /note="major polyA site"
ORIGIN      
        1 agacacggcc ctctcagccc cgaacggcgc gcgctgcccg gcgggcggga tccgggcgcg
       61 gccgaggcgg cggcgctgca ccccgggcct gggcccgcgc gactcccccg ggggcctctc
      121 ccacgctccg cgccccgccc cacccttcct tccttcctcc cctcggcgct cccgggagcg
      181 ctcggcagac gcccgagctt tgtgggcgcc cgcgggccca gcgcgagcct cgggcggccc
      241 aactttgcct ctcccggtcg gctcccgagc gggacttgtg aggcgtggcc gggtggagga
      301 ggcgcttgcg ggggcgtggg gctccccggc aggcgggagg cacggagcaa gtcgcactgg
      361 acaatgacct gggcagctcg gcgctgcgga cacttttagc tgagggcgcg ggcgggtcgg
      421 ctcctccgcg gctcctcggc cccacctgcg cggagagggc gggatgccag agccaggtgt
      481 cccggcgcgt taagggccct cgcagtcaga cgtccctgca ccggcgctcg cacccttagt
      541 cggcccggaa cgtctttttg cggacgccct cggagcagcc gcgatggcca gcaccaggag
      601 tatcgagctg gagcactttg aggaacggga caaaaggccg cggccggggt cgcggagagg
      661 ggcccccagc tcctccgggg gcagcagcag ctcgggcccc aaggggaacg ggctcatccc
      721 cagtccggcg cacagtgccc actgcagctt ctaccgcacg cggaccctgc aggccctcag
      781 ctcggagaag aaggccaaga aggcgcgctt ctaccggaac ggggaccgct acttcaaggg
      841 cctggtgttt gccatctcca gcgaccgctt ccggtccttc gatgcgctcc tcatagagct
      901 cacccgctcc ctgtcggaca acgtgaacct gccccagggt gtccgcacta tctacaccat
      961 cgacggcagc cggaaggtca ccagcctgga cgagctgctg gaaggtgaga gttacgtgtg
     1021 tgcatccaat gaaccatttc gtaaagtcga ttacaccaaa aatattaatc caaactggtc
     1081 tgtgaacatc aagggtggga catcccgagc gctggctgct gcctcctctg tgaaaagtga
     1141 agtaaaagaa agtaaagatt tcatcaaacc caagttagtg actgtgattc gaagtggagt
     1201 gaagcctaga aaagccgtgc ggatccttct gaataaaaag actgctcatt cctttgaaca
     1261 agtcttaaca gatatcaccg aagccattaa actagactca ggagtcgtca agaggctctg
     1321 caccctggat ggaaagcagg ttacttgtct gcaagacttt tttggtgatg acgatgtttt
     1381 tattgcatgt ggaccagaaa aatttcgtta tgcccaagat gactttgtcc tggatcatag
     1441 tgaatgtcgt gtcctgaagt catcttattc tcgatcctca gctgttaagt attctggatc
     1501 caaaagccct gggccctctc gacgcagcaa atcaccagct tcagttaatg gaactcccag
     1561 cagccaactt tctactccta aatctacgaa atcctccagt tcctctccaa ctagtccagg
     1621 aagtttcaga ggattaaagc agatttctgc tcatggcaga tcttcttcca atgtaaacgg
     1681 tggacctgag cttgaccgtt gcataagtcc tgaaggtgtg aatggaaaca gatgctctga
     1741 atcatcaact cttcttgaga aatacaaaat tggaaaggtc attggtgatg gcaattttgc
     1801 agtagtcaaa gagtgtatag acaggtccac tggaaaggag tttgccctaa agattataga
     1861 caaagccaaa tgttgtggaa aggaacacct gattgagaat gaagtgtcaa tactgcgccg
     1921 agtgaaacat cccaatatca ttatgctggt cgaggagatg gaaacagcaa ctgagctctt
     1981 tctggtgatg gaattggtca aaggtggaga tctctttgat gcaattactt cgtcgaccaa
     2041 gtacactgag agagatggca gtgccatggt gtacaactta gccaatgccc tcaggtatct
     2101 ccatggcctc agcatcgtgc acagagacat caaaccagag aatctcttgg tgtgtgaata
     2161 tcctgatgga accaagtctt tgaaactggg agactttggg cttgcgactg tggtagaagg
     2221 ccctttatac acagtctgtg gcacacccac ttatgtggct ccagaaatca ttgctgaaac
     2281 tggctatggc ctgaaggtgg acatttgggc agctggtgtg atcacataca tacttctctg
     2341 tggattccca ccattccgaa gtgagaacaa tctccaggaa gatctcttcg accagatctt
     2401 ggctgggaag ctggagtttc cggcccccta ctgggataac atcacggact ctgccaagga
     2461 attaatcagt caaatgcttc aggtaaatgt tgaagctcgg tgtaccgcgg gacaaatcct
     2521 gagtcacccc tgggtgtcag atgatgcctc ccaggagaat aacatgcaag ctgaggtgac
     2581 aggtaaacta aaacagcact ttaataatgc gctccccaaa cagaacagca ctaccaccgg
     2641 ggtctccgtc atcatgaaca cggctctaga taaggagggg cagattttct gcagcaagca
     2701 ctgtcaagac agcggcaggc ctgggatgga gcccatctct ccagttcctc cctcagtgga
     2761 ggagatccct gtgcctgggg aagcagtccc ggcccccacc cctccggaat ctcccacccc
     2821 ccaccctcct cccgctgccc cgggtggtga gcgggcagga acctggcgcc gccaccgaga
     2881 ctgagcctcc tgcagacggg cgaagccgcc tgctgcagcc caggaagcca gccctctgct
     2941 cggcctcgcc ggcctccctg ctgcaggcct ccctctcttc accgcctgcg cctgagttcg
     3001 cgggtcctcc gcaggccgcc tgggaaccgg agcctggcgt gccggagcct ggcctggtgc
     3061 tctgggctct gccttctggt tcctggaggc atcaaaggct gcatccgttc tgccaacagc
     3121 tgttcggaga gactcgttcc agatcatccc gtcattttca gtttgttgga cattttacag
     3181 cttcaccagg agaatgtgca actttattcc agcattcgat gcatttttat agaaacactt
     3241 tggaaacact ttggatgaac caaggccttt tccttattta agtagactca gaacactccc
     3301 tttcttttct tttctctctc tctctctctt ttttttttac gaaagactta gaattgcatt
     3361 tgtccttttg tgggtgtcct gtgagaggtg atatgggggc taagaggact ggctttctaa
     3421 tagaagaagt gagcgcctga gaggacaatt tggtcattgg acacggattg caggctttga
     3481 gaagcgctca gaggcccagg gcggcgggct cagccattcg gcttggggca ccaggctccc
     3541 cagagacaat gctcagtatt cattcataca cagacgatgg aagaagccac ttcttccctg
     3601 ggcggtgtgg gtttccccca gctcttccca cacgtgtgtt aggaaatgcc cgtgaacttg
     3661 ccctctgggc tttttaatga gaggcttggc gcatgcggca cccagcggct gcttccctgc
     3721 aagccagcga cttgccgagc agaatgagct ctgctcctga gccccggtag ctgcttcctc
     3781 atctgcattt catctggagc agggcaggta accacaggag gaggcatgcc cgaggcagcc
     3841 aggactgggc gggaaggtgc tccctggata tactctgtga accttctgga aggctgctgg
     3901 cagtttttcc tttttgtcca ccaccctgct ctttttaata attgtacata atccgtgtat
     3961 ttgttttacc tgctcatctt ctaaactggc gagccctata gttcgttctc attgttagat
     4021 tttgcctttt acaagtgtcc ccaacctgca ataaactttt ccctcttgaa aaaaa
//
