LOCUS       NM_001330072            8295 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens doublecortin like kinase 1 (DCLK1), transcript variant
            6, mRNA.
ACCESSION   NM_001330072 XM_006719893
VERSION     NM_001330072.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Afshar-Sterle,S., Carli,A.L.E., O'Keefe,R., Tse,J., Fischer,S.,
            Azimpour,A.I., Baloyan,D., Elias,L., Thilakasiri,P., Patel,O.,
            Ferguson,F.M., Eissmann,M.F., Chand,A.L., Gray,N.S., Busuttil,R.,
            Boussioutas,A., Lucet,I.S., Ernst,M. and Buchert,M.
  TITLE     DCLK1 induces a pro-tumorigenic phenotype to drive gastric cancer
            progression
  JOURNAL   Sci Signal 17 (854), eabq4888 (2024)
   PUBMED   39288218
  REMARK    GeneRIF: DCLK1 induces a pro-tumorigenic phenotype to drive gastric
            cancer progression.
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Fukushima,N.M., Adachi,T., Tanaka,T., Matsushima,H., Imamura,H.,
            Hara,T., Soyama,A., Hidaka,M., Kanetaka,K. and Eguchi,S.
  TITLE     DCLK1 Expression and its Functional Significance in Intrahepatic
            Cholangiocarcinoma and Bil-IN Stage
  JOURNAL   Anticancer Res 44 (6), 2417-2424 (2024)
   PUBMED   38821583
  REMARK    GeneRIF: DCLK1 Expression and its Functional Significance in
            Intrahepatic Cholangiocarcinoma and Bil-IN Stage.
REFERENCE   3  (bases 1 to 8295)
  AUTHORS   Ye,L., Liu,B., Huang,J., Zhao,X., Wang,Y., Xu,Y. and Wang,S.
  TITLE     DCLK1 and its oncogenic functions: A promising therapeutic target
            for cancers
  JOURNAL   Life Sci 336, 122294 (2024)
   PUBMED   38007147
  REMARK    GeneRIF: DCLK1 and its oncogenic functions: A promising therapeutic
            target for cancers.
            Review article
REFERENCE   4  (bases 1 to 8295)
  AUTHORS   Chen,X.Y., Kao,C., Peng,S.W., Chang,J.H., Lee,Y.L., Laiman,V.,
            Chung,K.F., Bhavsar,P.K., Heriyanto,D.S., Chuang,K.J. and
            Chuang,H.C.
  TITLE     Role of DCLK1/Hippo pathway in type II alveolar epithelial cells
            differentiation in acute respiratory distress syndrome
  JOURNAL   Mol Med 29 (1), 159 (2023)
   PUBMED   37996782
  REMARK    GeneRIF: Role of DCLK1/Hippo pathway in type II alveolar epithelial
            cells differentiation in acute respiratory distress syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8295)
  AUTHORS   Yasodha,K., Lizha Mary,L., Surajit,P. and Satish,R.
  TITLE     Exosomes from metastatic colon cancer cells drive the proliferation
            and migration of primary colon cancer through increased expression
            of cancer stem cell markers CD133 and DCLK1
  JOURNAL   Tissue Cell 84, 102163 (2023)
   PUBMED   37487255
  REMARK    GeneRIF: Exosomes from metastatic colon cancer cells drive the
            proliferation and migration of primary colon cancer through
            increased expression of cancer stem cell markers CD133 and DCLK1.
REFERENCE   6  (bases 1 to 8295)
  AUTHORS   Lin,P.T., Gleeson,J.G., Corbo,J.C., Flanagan,L. and Walsh,C.A.
  TITLE     DCAMKL1 encodes a protein kinase with homology to doublecortin that
            regulates microtubule polymerization
  JOURNAL   J Neurosci 20 (24), 9152-9161 (2000)
   PUBMED   11124993
REFERENCE   7  (bases 1 to 8295)
  AUTHORS   Matsumoto,N., Pilz,D.T. and Ledbetter,D.H.
  TITLE     Genomic structure, chromosomal mapping, and expression pattern of
            human DCAMKL1 (KIAA0369), a homologue of DCX (XLIS)
  JOURNAL   Genomics 56 (2), 179-183 (1999)
   PUBMED   10051403
REFERENCE   8  (bases 1 to 8295)
  AUTHORS   Sossey-Alaoui,K. and Srivastava,A.K.
  TITLE     DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is
            similar to doublecortin (DCX)
  JOURNAL   Genomics 56 (1), 121-126 (1999)
   PUBMED   10036192
REFERENCE   9  (bases 1 to 8295)
  AUTHORS   Omori,Y., Suzuki,M., Ozaki,K., Harada,Y., Nakamura,Y., Takahashi,E.
            and Fujiwara,T.
  TITLE     Expression and chromosomal localization of KIAA0369, a putative
            kinase structurally related to Doublecortin
  JOURNAL   J Hum Genet 43 (3), 169-177 (1998)
   PUBMED   9747029
REFERENCE   10 (bases 1 to 8295)
  AUTHORS   Nedivi,E., Hevroni,D., Naot,D., Israeli,D. and Citri,Y.
  TITLE     Numerous candidate plasticity-related genes revealed by
            differential cDNA cloning
  JOURNAL   Nature 363 (6431), 718-722 (1993)
   PUBMED   8515813
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA154341.1, BC152456.1,
            AK295777.1, DC350316.1, AL157760.9, AL139083.15 and BM679448.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001330072.1.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmodulin-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. The encoded
            protein is involved in several different cellular processes,
            including neuronal migration, retrograde transport, neuronal
            apoptosis and neurogenesis. This gene is up-regulated by
            brain-derived neurotrophic factor and associated with memory and
            general cognitive abilities. Multiple transcript variants generated
            by two alternative promoter usage and alternative splicing have
            been reported, but the full-length nature and biological validity
            of some variants have not been defined. These variants encode
            different isoforms, which are differentially expressed and have
            different kinase activities.[provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (6) differs in the 5' UTR and
            lacks an alternate exon in the 3' coding region, resulting in a
            frameshift compared to variant 1. Both variants 5 and 6 encode the
            same isoform (5), which is longer and has a distinct C-terminus
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.95372.1,
                                           SRR14372079.1508494.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-493               DA154341.1         82-574
            494-1436            BC152456.1         517-1459
            1437-1947           AK295777.1         466-976
            1948-2527           DC350316.1         1-580
            2528-3175           AK295777.1         1631-2278
            3176-6106           AL157760.9         2001-4931           c
            6107-7281           AL139083.15        160907-162081       c
            7282-7958           BM679448.1         19-695              c
            7959-8295           AL139083.15        159893-160229       c
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.3"
     gene            1..8295
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="doublecortin like kinase 1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
     exon            1..170
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            171..565
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..2412
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /EC_number="2.7.11.1"
                     /note="isoform 5 is encoded by transcript variant 6;
                     serine/threonine-protein kinase DCLK1; doublecortin-like
                     and CAM kinase-like 1; doublecortin domain-containing
                     protein 3A"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK1 isoform 5"
                     /protein_id="NP_001317001.1"
                     /db_xref="CCDS:CCDS81762.1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
                     /translation="MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRT
                     RTLQTLSSEKKAKKVRFYRNGDRYFKGIVYAISPDRFRSFEALLADLTRTLSDNVNLP
                     QGVRTIYTIDGLKKISSLDQLVEGESYVCGSIEPFKKLEYTKNVNPNWSVNVKTTSAS
                     RAVSSLATAKGSPSEVRENKDFIRPKLVTIIRSGVKPRKAVRILLNKKTAHSFEQVLT
                     DITDAIKLDSGVVKRLYTLDGKQVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESEC
                     RVVKSTSYTKIASSSRRSTTKSPGPSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPS
                     PTSPGSLRKQRSSQHGGSSTSLASTKVCSSMDENDGPGEEVSEEGFQIPATITERYKV
                     GRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVL
                     LIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIV
                     HRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGL
                     KVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELI
                     TMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGV
                     SVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF
                     "
     exon            566..912
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1012
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1129
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1224
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1309
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1418
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1476
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1596
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1743
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1877
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1955
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2052
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2133
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2247
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..8295
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5590..5595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     polyA_site      5607
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="major polyA site"
     regulatory      7939..7944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     regulatory      7944..7949
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAT"
     polyA_site      7962
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
     polyA_site      7966
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
ORIGIN      
        1 gaacagatcc agctcttccc aatgtggggg gcaccagagt gcatagcttg ggagggttgg
       61 tcatccgaag aggcactgcg tgggtgcatc ccgggcaaaa aggatgagaa ggtgatccac
      121 tggcttccat accctgggaa aggtgtcaga ccgtgaggtc acatcaaaag gtcctacttg
      181 aagtccatca tgtccttcgg cagagacatg gagctggagc acttcgacga gcgggataag
      241 gcgcagagat acagccgagg gtcgcgggtg aacggcctgc cgagcccgac gcacagcgcc
      301 cactgcagct tctaccgcac ccgcacgctg cagacgctca gctccgagaa gaaggccaag
      361 aaagttcgtt tctatcgaaa cggagatcga tacttcaaag ggattgtgta tgccatctcc
      421 ccagaccggt tccgatcttt tgaggccctg ctggctgatt tgacccgaac tctgtcggat
      481 aacgtgaatt tgccccaggg agtgagaaca atctacacca ttgatgggct caagaagatt
      541 tccagcctgg accaactggt ggaaggagag agttatgtat gtggctccat agagcccttc
      601 aagaaactgg agtacaccaa gaatgtgaac cccaactggt cggtgaacgt caagaccacc
      661 tcggcttctc gggcagtgtc ttcactggcc actgccaaag gaagcccttc agaggtgcga
      721 gagaataagg atttcattcg gcccaagctg gtcaccatca tcagaagtgg cgtgaagcca
      781 cggaaagctg tcaggattct gctgaacaag aaaacggctc attcctttga gcaggtcctc
      841 accgatatca ccgatgccat caagctggac tcgggagtgg tgaaacgcct gtacacgttg
      901 gatgggaaac aggtgatgtg ccttcaggac ttttttggtg atgatgacat ttttattgca
      961 tgtggaccgg agaagttccg ttaccaggat gatttcttgc tagatgaaag tgaatgtcga
     1021 gtggtaaagt ccacttctta caccaaaata gcttcatcat cccgcaggag caccaccaag
     1081 agcccaggac cgtccaggcg tagcaagtcc cctgcctcca ccagctcagt taatggaacc
     1141 cctggtagtc agctctctac tccgcgctca ggcaagtcgc caagcccatc acccaccagc
     1201 ccaggaagcc tgcggaagca gaggagctct cagcatggcg gctcctctac gtcacttgcg
     1261 tccaccaaag tctgcagctc gatggatgag aacgatggcc ctggagaaga agtgtcggag
     1321 gaaggcttcc agattccagc tacaataaca gaacgatata aagtcggaag aacaatagga
     1381 gatggaaatt ttgctgttgt caaggaatgt gtagaaagat cgactgctag agagtatgct
     1441 ctgaaaatta tcaagaaaag caaatgtcga ggcaaagagc acatgatcca gaatgaagtg
     1501 tctattttaa gaagagtgaa gcatcccaat atcgttcttc tgattgagga gatggatgtg
     1561 ccaactgaac tgtatcttgt catggaatta gtaaaggggg gagacctttt tgatgccatt
     1621 acttccacta acaaatacac cgagagagac gccagtggga tgctgtacaa cctagccagc
     1681 gccatcaaat acctgcatag cctgaacatc gtccaccgtg atatcaagcc agagaacctg
     1741 ctggtgtatg agcaccaaga tggcagcaaa tcactgaagc tgggtgactt tggactggcc
     1801 accattgtag acggccccct gtacacagtc tgtggcaccc caacatacgt ggctccagaa
     1861 atcattgcag agactggata cggcctcaag gtggacatct gggcagcagg tgtaatcact
     1921 tatatcctgc tgtgtggttt ccctccattc cgtggaagtg gtgatgacca ggaggtgctt
     1981 tttgatcaga ttttgatggg gcaggtggac tttccttctc catactggga taatgtttcc
     2041 gattctgcaa aggagctcat taccatgatg ctgttggtcg atgtagatca gcgattttct
     2101 gctgttcaag tacttgagca tccctgggtt aatgatgatg gcctcccaga aaatgaacat
     2161 cagctgtcag tagctggaaa gataaagaag catttcaaca caggccccaa gccgaatagc
     2221 acagcagctg gagtttctgt catagcaacc accgctcttg ataaggagag gcaggttttc
     2281 cgacgaagac gcaaccagga tgtgaggagc cggtacaagg cgcagccagc tcctcccgaa
     2341 ctcaactcgg aatcggaaga ctactcccca agctcctccg agactgttcg ctcccctaac
     2401 tcgccctttt aataagaccc ttttactcaa agtcctagct taaccctttg agactctgag
     2461 atttttttcc cccaaatttg tgtaaaacag tttcatctga tctatctagc gctcaatgct
     2521 tgaatggcag aactgaaagt gttttcaggt atctttgtag cggtttccct ttactgaata
     2581 agatgacacg tggtgattgt gaagatggta atttgctgct aatagagtcc tcaaagggtt
     2641 aaggccaatt tgcaattttt ttttaaactt agaagcaatg aatgttttca tcagtcaagc
     2701 taggatctgc agtatgtaat atagcacttg ttaaccctct gagtgcatag aattttattg
     2761 agaattcttg tttgggaatt tttcaggcct ttggatgtat acacacatgt ttcttgattt
     2821 tactgcagat caaggggtgt tgttagatgc tgaaatgtcc agaaaagaag gacatttaga
     2881 atgatatctt gtttgtcctt ttctgtgggt ttagaacgtg gcaggtttat aacttcgaca
     2941 cacgcacggt tctttcttct tcacaatcct attcagaaac agattttttt tttcattaga
     3001 gatatgactg tcagttgcag tgagttctgc atcccaagtg gagggaattg ggtttgtggc
     3061 aaagagcttg acccaggaaa tagatggtgc cccccaaatt gtctccacat gaagatgtac
     3121 tgatgacgcc ccagaaatgc tgcttccata tcagctgctg ctagcgccag cgcagactct
     3181 cagggagtca ccacagcttg tcttgtgctt ggtgagtgag ggtctctcta ctcagtgtca
     3241 gacatctaca ggaaagaaac aactggtgga aaagagcaat aaattgcccg gtgctctgca
     3301 gggctggaat ttcaaacaga aagagggaat aagatcctgt gatttttctc acctgctttt
     3361 ccacgcactg tggtcatcac tgtgcaatct acatctagta tgaaatccac acataggaga
     3421 gctggggcac aaggggactg gaggcagttg ctttgcaaga tggctgagga gaaagcacac
     3481 tgggaacaca atccagaatg ttctaacaat aagttttcag tgaataaacc actggcaaga
     3541 caattccatg tgcaccttta ggttacctat atagtctcct aggaagatca ggatgaaaga
     3601 cctagatgat acccctgagg ataaaacctc catcccctaa aatgattttt tttaaatacc
     3661 actgtcttta gctgtccagg aggtcagagt gttttttctg tctttgggcc aagtcctgtc
     3721 tgagacctgt attttcactc ttgttaccaa atctatctcc ctagtgcagt gtctccaggc
     3781 ctgagtttct tctggaacag attccatttt agaatgggga ttcacaggtt ctgtgcatca
     3841 ccacagtgct cagagaggat tctcctgggg tgtcttagag gcaggtgccc aactcaaatg
     3901 tattcccaag gtttgctggg ctctgggatc cacgagacaa ccagagaggg atatctcatg
     3961 aaatttgcat ctggtggctg aacagtacct atgttctctg ttttgaatat actttaatac
     4021 ctgagagtct taaaatttgt gaacaacgtt tctatagtcc tttattttca aatgcacatt
     4081 gatcttcact tgctgcattt ttactcttca accctgaaac tatggtctac attaatatgg
     4141 atttttaaat cacatgtcat tacttttgca acaccatcac caaaattttt tgctctttta
     4201 catttaggtt catctctgtg gtctgtgttg tcctgacatg taaaaagcat atcgtttatt
     4261 gaggtttttt tccccccctt ttagagcatc cggaagtgat aacacgcaaa atcacaaagt
     4321 agcataaatc agtaaattag ttgagttgtt tttggggggg aggtgggggt agggggcaca
     4381 gaacaccaga aagagtgttg gtgtgtaggt agattccata ttaatgagga acactgaact
     4441 agttggaaat tactgctttc tctagaaata taaagcaaag cactattcca aggctatgga
     4501 gtagctctac agcctggcct caactctaaa agtgtgaaga atgcaatggg cagagaccta
     4561 cctgcagtgg actgtcattt tcctttcttt ctctgaatta ctgctttttc tgtgggcatt
     4621 aactatattg ctacagcatc tagtgtactg agcctgcggt gcatggctca ggccttttcc
     4681 catcgacgtc tagggggact ctggaccgtg tgaagctagg gggtgtttct cagcacactg
     4741 cagaagggca gctcagaaga atgcagggcc cattcagcat ggggatccca gcacatcact
     4801 gtagaatttg agtgatctat gctgaataaa cagtggaatg tgaccagtca agtagaaatc
     4861 ttgagtaatc agatggaatg caatctttct aacattaagc taccaagatc ctgaatgtca
     4921 gagatgtact cagagggtta acagacaagc acaaggcatg ctgactacat tggtgtatcc
     4981 agattgcttt gcttttagcc agtgctttct aatttttttc tcgacattct tgggatagtt
     5041 caagtttgaa ataattaagt ggtggtgttc tttaaggaat ttctataacc aaattgatct
     5101 tatttttgat ttcacttatc atagaacaaa tatgtatcat tatggcagtg tatctatgta
     5161 attatcaatt taatcatcac caccggtgtt tccatatttt ttcccaagta tttaatatag
     5221 ctctcttatg gtggtggcct ggtgatgggg accgtctttc ttttactgac acatgaccaa
     5281 tcatatggta ttttcaaggg aattttaaga ttcatctttt cagtttgata gtagactagt
     5341 taaggaagaa ctctttcatt acttgcatcg tgtaaatcat ctctgtagac atgtgttcat
     5401 attaatgaac acattttttc tcaacattgt agcagaaatc attttattcg tcatgatcaa
     5461 tgaatatgtg atttgctcca gatcgttaga aggaaaagta agatttcagt catcaaaaat
     5521 gtttttaccg tagccctcat ctaacttaca cgtggtgcat attaaaataa gcagagaaaa
     5581 aaaaatgtga ataaactact gaaaacactt ggtgttttgt gttcaatgag accttcctgc
     5641 aacctgctcc ccatgggtgg cagttaacag gcccatcaga tattgttgaa agaaagcaat
     5701 atatccatga atgaaggcta aaattgcaat cctttaccct ttgaggcata tttcagttga
     5761 aaacaaaaag aaaagaaaat ttggcttaga gggtcacaga gctcccatat gaccaagtct
     5821 caagcacatt aaatcatggt tgtttactgg ccaagggcgt ccactagaca actctatccc
     5881 ttgcgctgaa gctcaatcgt gctgagggag agctttctta atattactgt gttgctctta
     5941 gcccttctct gggttaggat ctgtcagcat ttctatgata aactcctatt ctcaaaggtt
     6001 tttaatttga ccataaaaat gtgccccagg ctgaagtttg ctatacaggg ctgtaccaaa
     6061 gagtgaaggt ttacttcctt ctctttccaa cttcttcccc attctccaag gaaaagaaca
     6121 acaaaaaaat ctggtatggt ccctccttaa tagtgatttc agaattttgg aaagcaccaa
     6181 gatccaagat ggtagtttta atgtagttac tcattcgcac acatttttta aatttaatgg
     6241 gtcacctggc atatattgta gataacatat cttttctata atttgtaagt caataatttt
     6301 tttaactgct acatgatatt tttttttgcc caaagatttt aaaagacttg aagttggtca
     6361 gttcaaaact cagatttttc tacacattgt ctgccatgtc cattaggagt ttggggaaaa
     6421 tactctcaca cagaccctta ctttgcatgc agtttagagg gtaagatacg tgcttctttt
     6481 ggggataaag atttccttac ttaattgtca aatttcatgg agccattcta gtctgttggg
     6541 gaaaatagtg attaaaagca cttccaaaat taacattttt tgacaattca gatatgaaaa
     6601 gaagcagggg aaaataatac actttactct tttcttgctt aaaggcaaac aaatcaatga
     6661 aacttgagga cacactaaac atttgataac tgcaaatgtg ctttaaaaat tggttcaatg
     6721 gtgcttacac atgaaacggt aacaaatggg gttcctagga cgtcagaagg aatctttagt
     6781 ttgtatgtaa ttacacacta gaggaggagg tgcttttaag ccagtctttt atttttaatc
     6841 atctcaaata tgcaaccata catgcagtaa cattaagggt cgtaaactgg tgggaaacag
     6901 gaacttcagt ggagaggctt aaatgcctct ggttagagtg ggggtttttg tttgtttgtt
     6961 tattgttggg tttcaacact gagcatcatt tctgtgatca agtttctaac tggcatgtgt
     7021 tttgatcatg aggtttacca tatcttgccc atacagacaa atgagagatc tagtttcatt
     7081 ttgttcccta aagaaagaac actctctaaa attaaatcat acctgtaaat ttcttcagca
     7141 tttgtttctg ttcaatgaaa ttgagaccct taatgttgct ttaatgtaaa attgaatatt
     7201 ttgtctgtga tatactttaa taatttaaag taagtaatag ttctaaagtc ttcactgttg
     7261 ctactaagag aaaatagaat tttaaaggtg atgataaaga tgctataatg tcagttcact
     7321 ccagtccaat caaatgtagt aagaaaaagt ccttgaatag ttctctaggg acaatttctc
     7381 acttgccatt gacattaatc tttggtgtat tctcagaaaa aataaaaaga aattgaaact
     7441 ggtccaaggt tatagtcata tcctcgataa cttttgaaaa aaaattttat taggaaatta
     7501 atactagcct ttttcattct ggctgaaaga aaattattaa aggattagtt gagtgtgaaa
     7561 ttcaacagta ttttgctcat acatactaaa aaggtgcgta gggacttggc gcatttaaac
     7621 aagtttctga aaggtttcaa tttgactcaa gaaaaaaatt caatatttct tttgaaaata
     7681 ctgaatttat cacttgctgc atggatcaga tggcataggt taatctttga ttttcagaat
     7741 cctaatgaaa taactttcaa acaatttgtg tccttaatta aaggtggaat gagatccaat
     7801 ttttccccct aatccttcag tttaagctga tacatgtagg ttaatgtgga atgaaatcat
     7861 ctgtgatata ttatgttcat ttatcaactg agcttttttg atgttgcctg tttttatgta
     7921 aaacatgttc ttaaagttaa taaaataata gtacttggtg tatgaactac tacataatac
     7981 cttagctgaa gggtacgctc ctcagttcat ttgccatatt ggagtaaaat ttcaccttag
     8041 ttcaattcac aattgacaca ttcagttcac gaaggaatag cccagctgtg ttcttagggg
     8101 gaattctctc tatgcaaggc tttaaaaatt agtcatgcgg tcagagtgta gctttcccac
     8161 atgtcctcca tagcacagtg tgtatttcat gagcataatt caaaaacagc tatttcaaaa
     8221 atgtcccttg tcatatatta cgttcctgcc tgggccactg acttggataa atacaaataa
     8281 accaaatacc attca
//
