LOCUS       NM_001330071            8394 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens doublecortin like kinase 1 (DCLK1), transcript variant
            5, mRNA.
ACCESSION   NM_001330071 XM_005266592
VERSION     NM_001330071.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Afshar-Sterle,S., Carli,A.L.E., O'Keefe,R., Tse,J., Fischer,S.,
            Azimpour,A.I., Baloyan,D., Elias,L., Thilakasiri,P., Patel,O.,
            Ferguson,F.M., Eissmann,M.F., Chand,A.L., Gray,N.S., Busuttil,R.,
            Boussioutas,A., Lucet,I.S., Ernst,M. and Buchert,M.
  TITLE     DCLK1 induces a pro-tumorigenic phenotype to drive gastric cancer
            progression
  JOURNAL   Sci Signal 17 (854), eabq4888 (2024)
   PUBMED   39288218
  REMARK    GeneRIF: DCLK1 induces a pro-tumorigenic phenotype to drive gastric
            cancer progression.
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Fukushima,N.M., Adachi,T., Tanaka,T., Matsushima,H., Imamura,H.,
            Hara,T., Soyama,A., Hidaka,M., Kanetaka,K. and Eguchi,S.
  TITLE     DCLK1 Expression and its Functional Significance in Intrahepatic
            Cholangiocarcinoma and Bil-IN Stage
  JOURNAL   Anticancer Res 44 (6), 2417-2424 (2024)
   PUBMED   38821583
  REMARK    GeneRIF: DCLK1 Expression and its Functional Significance in
            Intrahepatic Cholangiocarcinoma and Bil-IN Stage.
REFERENCE   3  (bases 1 to 8394)
  AUTHORS   Ye,L., Liu,B., Huang,J., Zhao,X., Wang,Y., Xu,Y. and Wang,S.
  TITLE     DCLK1 and its oncogenic functions: A promising therapeutic target
            for cancers
  JOURNAL   Life Sci 336, 122294 (2024)
   PUBMED   38007147
  REMARK    GeneRIF: DCLK1 and its oncogenic functions: A promising therapeutic
            target for cancers.
            Review article
REFERENCE   4  (bases 1 to 8394)
  AUTHORS   Chen,X.Y., Kao,C., Peng,S.W., Chang,J.H., Lee,Y.L., Laiman,V.,
            Chung,K.F., Bhavsar,P.K., Heriyanto,D.S., Chuang,K.J. and
            Chuang,H.C.
  TITLE     Role of DCLK1/Hippo pathway in type II alveolar epithelial cells
            differentiation in acute respiratory distress syndrome
  JOURNAL   Mol Med 29 (1), 159 (2023)
   PUBMED   37996782
  REMARK    GeneRIF: Role of DCLK1/Hippo pathway in type II alveolar epithelial
            cells differentiation in acute respiratory distress syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8394)
  AUTHORS   Yasodha,K., Lizha Mary,L., Surajit,P. and Satish,R.
  TITLE     Exosomes from metastatic colon cancer cells drive the proliferation
            and migration of primary colon cancer through increased expression
            of cancer stem cell markers CD133 and DCLK1
  JOURNAL   Tissue Cell 84, 102163 (2023)
   PUBMED   37487255
  REMARK    GeneRIF: Exosomes from metastatic colon cancer cells drive the
            proliferation and migration of primary colon cancer through
            increased expression of cancer stem cell markers CD133 and DCLK1.
REFERENCE   6  (bases 1 to 8394)
  AUTHORS   Lin,P.T., Gleeson,J.G., Corbo,J.C., Flanagan,L. and Walsh,C.A.
  TITLE     DCAMKL1 encodes a protein kinase with homology to doublecortin that
            regulates microtubule polymerization
  JOURNAL   J Neurosci 20 (24), 9152-9161 (2000)
   PUBMED   11124993
REFERENCE   7  (bases 1 to 8394)
  AUTHORS   Matsumoto,N., Pilz,D.T. and Ledbetter,D.H.
  TITLE     Genomic structure, chromosomal mapping, and expression pattern of
            human DCAMKL1 (KIAA0369), a homologue of DCX (XLIS)
  JOURNAL   Genomics 56 (2), 179-183 (1999)
   PUBMED   10051403
REFERENCE   8  (bases 1 to 8394)
  AUTHORS   Sossey-Alaoui,K. and Srivastava,A.K.
  TITLE     DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is
            similar to doublecortin (DCX)
  JOURNAL   Genomics 56 (1), 121-126 (1999)
   PUBMED   10036192
REFERENCE   9  (bases 1 to 8394)
  AUTHORS   Omori,Y., Suzuki,M., Ozaki,K., Harada,Y., Nakamura,Y., Takahashi,E.
            and Fujiwara,T.
  TITLE     Expression and chromosomal localization of KIAA0369, a putative
            kinase structurally related to Doublecortin
  JOURNAL   J Hum Genet 43 (3), 169-177 (1998)
   PUBMED   9747029
REFERENCE   10 (bases 1 to 8394)
  AUTHORS   Nedivi,E., Hevroni,D., Naot,D., Israeli,D. and Citri,Y.
  TITLE     Numerous candidate plasticity-related genes revealed by
            differential cDNA cloning
  JOURNAL   Nature 363 (6431), 718-722 (1993)
   PUBMED   8515813
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL160392.12, DB453639.2,
            HY138649.1, BC152456.1, AK295777.1, DC350316.1, AL157760.9,
            AL139083.15 and BM679448.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001330071.1.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmodulin-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. The encoded
            protein is involved in several different cellular processes,
            including neuronal migration, retrograde transport, neuronal
            apoptosis and neurogenesis. This gene is up-regulated by
            brain-derived neurotrophic factor and associated with memory and
            general cognitive abilities. Multiple transcript variants generated
            by two alternative promoter usage and alternative splicing have
            been reported, but the full-length nature and biological validity
            of some variants have not been defined. These variants encode
            different isoforms, which are differentially expressed and have
            different kinase activities.[provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (5) lacks an alternate exon in the
            3' coding region, resulting in a frameshift compared to variant 1.
            Both variants 5 and 6 encode the same isoform (5), which is longer
            and has a distinct C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3322987.1,
                                           SRR14038192.1494343.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360631.8/ ENSP00000353846.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AL160392.12        13533-13537         c
            6-107               DB453639.2         3-104
            108-130             HY138649.1         102-124
            131-1535            BC152456.1         55-1459
            1536-2046           AK295777.1         466-976
            2047-2626           DC350316.1         1-580
            2627-3274           AK295777.1         1631-2278
            3275-6205           AL157760.9         2001-4931           c
            6206-7380           AL139083.15        160907-162081       c
            7381-8057           BM679448.1         19-695              c
            8058-8394           AL139083.15        159893-160229       c
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.3"
     gene            1..8394
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="doublecortin like kinase 1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
     exon            1..269
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            270..664
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..2511
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /EC_number="2.7.11.1"
                     /note="isoform 5 is encoded by transcript variant 5;
                     serine/threonine-protein kinase DCLK1; doublecortin-like
                     and CAM kinase-like 1; doublecortin domain-containing
                     protein 3A"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK1 isoform 5"
                     /protein_id="NP_001317000.1"
                     /db_xref="CCDS:CCDS81762.1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
                     /translation="MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRT
                     RTLQTLSSEKKAKKVRFYRNGDRYFKGIVYAISPDRFRSFEALLADLTRTLSDNVNLP
                     QGVRTIYTIDGLKKISSLDQLVEGESYVCGSIEPFKKLEYTKNVNPNWSVNVKTTSAS
                     RAVSSLATAKGSPSEVRENKDFIRPKLVTIIRSGVKPRKAVRILLNKKTAHSFEQVLT
                     DITDAIKLDSGVVKRLYTLDGKQVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESEC
                     RVVKSTSYTKIASSSRRSTTKSPGPSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPS
                     PTSPGSLRKQRSSQHGGSSTSLASTKVCSSMDENDGPGEEVSEEGFQIPATITERYKV
                     GRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVL
                     LIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIV
                     HRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGL
                     KVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELI
                     TMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGV
                     SVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF
                     "
     exon            665..1011
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1111
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1228
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1323
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1408
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1517
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1575
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1695
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1842
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1976
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2054
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2151
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2232
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2346
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..8394
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5689..5694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     polyA_site      5706
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="major polyA site"
     regulatory      8038..8043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     regulatory      8043..8048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAT"
     polyA_site      8061
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
     polyA_site      8065
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
ORIGIN      
        1 gccgccgccc tcctctggag agagaggctg gagtgaggct gtgcgaagcg ccgcatttca
       61 atgaggacgg gccgaggcac atccctgcac tagtggccgc aaccgaggcg ccgcgctcca
      121 gcagctgctg ccgcccagcc cggccccgcc gccgcccccc agccctgcag ccccgcagcc
      181 ccggccgcgc ccagcccggc gaggacagca ccaggaggcg gcccccagcg cggccacaaa
      241 gacccccggc ggcgtctctc cgcggaccgg tcctacttga agtccatcat gtccttcggc
      301 agagacatgg agctggagca cttcgacgag cgggataagg cgcagagata cagccgaggg
      361 tcgcgggtga acggcctgcc gagcccgacg cacagcgccc actgcagctt ctaccgcacc
      421 cgcacgctgc agacgctcag ctccgagaag aaggccaaga aagttcgttt ctatcgaaac
      481 ggagatcgat acttcaaagg gattgtgtat gccatctccc cagaccggtt ccgatctttt
      541 gaggccctgc tggctgattt gacccgaact ctgtcggata acgtgaattt gccccaggga
      601 gtgagaacaa tctacaccat tgatgggctc aagaagattt ccagcctgga ccaactggtg
      661 gaaggagaga gttatgtatg tggctccata gagcccttca agaaactgga gtacaccaag
      721 aatgtgaacc ccaactggtc ggtgaacgtc aagaccacct cggcttctcg ggcagtgtct
      781 tcactggcca ctgccaaagg aagcccttca gaggtgcgag agaataagga tttcattcgg
      841 cccaagctgg tcaccatcat cagaagtggc gtgaagccac ggaaagctgt caggattctg
      901 ctgaacaaga aaacggctca ttcctttgag caggtcctca ccgatatcac cgatgccatc
      961 aagctggact cgggagtggt gaaacgcctg tacacgttgg atgggaaaca ggtgatgtgc
     1021 cttcaggact tttttggtga tgatgacatt tttattgcat gtggaccgga gaagttccgt
     1081 taccaggatg atttcttgct agatgaaagt gaatgtcgag tggtaaagtc cacttcttac
     1141 accaaaatag cttcatcatc ccgcaggagc accaccaaga gcccaggacc gtccaggcgt
     1201 agcaagtccc ctgcctccac cagctcagtt aatggaaccc ctggtagtca gctctctact
     1261 ccgcgctcag gcaagtcgcc aagcccatca cccaccagcc caggaagcct gcggaagcag
     1321 aggagctctc agcatggcgg ctcctctacg tcacttgcgt ccaccaaagt ctgcagctcg
     1381 atggatgaga acgatggccc tggagaagaa gtgtcggagg aaggcttcca gattccagct
     1441 acaataacag aacgatataa agtcggaaga acaataggag atggaaattt tgctgttgtc
     1501 aaggaatgtg tagaaagatc gactgctaga gagtatgctc tgaaaattat caagaaaagc
     1561 aaatgtcgag gcaaagagca catgatccag aatgaagtgt ctattttaag aagagtgaag
     1621 catcccaata tcgttcttct gattgaggag atggatgtgc caactgaact gtatcttgtc
     1681 atggaattag taaagggggg agaccttttt gatgccatta cttccactaa caaatacacc
     1741 gagagagacg ccagtgggat gctgtacaac ctagccagcg ccatcaaata cctgcatagc
     1801 ctgaacatcg tccaccgtga tatcaagcca gagaacctgc tggtgtatga gcaccaagat
     1861 ggcagcaaat cactgaagct gggtgacttt ggactggcca ccattgtaga cggccccctg
     1921 tacacagtct gtggcacccc aacatacgtg gctccagaaa tcattgcaga gactggatac
     1981 ggcctcaagg tggacatctg ggcagcaggt gtaatcactt atatcctgct gtgtggtttc
     2041 cctccattcc gtggaagtgg tgatgaccag gaggtgcttt ttgatcagat tttgatgggg
     2101 caggtggact ttccttctcc atactgggat aatgtttccg attctgcaaa ggagctcatt
     2161 accatgatgc tgttggtcga tgtagatcag cgattttctg ctgttcaagt acttgagcat
     2221 ccctgggtta atgatgatgg cctcccagaa aatgaacatc agctgtcagt agctggaaag
     2281 ataaagaagc atttcaacac aggccccaag ccgaatagca cagcagctgg agtttctgtc
     2341 atagcaacca ccgctcttga taaggagagg caggttttcc gacgaagacg caaccaggat
     2401 gtgaggagcc ggtacaaggc gcagccagct cctcccgaac tcaactcgga atcggaagac
     2461 tactccccaa gctcctccga gactgttcgc tcccctaact cgccctttta ataagaccct
     2521 tttactcaaa gtcctagctt aaccctttga gactctgaga tttttttccc ccaaatttgt
     2581 gtaaaacagt ttcatctgat ctatctagcg ctcaatgctt gaatggcaga actgaaagtg
     2641 ttttcaggta tctttgtagc ggtttccctt tactgaataa gatgacacgt ggtgattgtg
     2701 aagatggtaa tttgctgcta atagagtcct caaagggtta aggccaattt gcaatttttt
     2761 tttaaactta gaagcaatga atgttttcat cagtcaagct aggatctgca gtatgtaata
     2821 tagcacttgt taaccctctg agtgcataga attttattga gaattcttgt ttgggaattt
     2881 ttcaggcctt tggatgtata cacacatgtt tcttgatttt actgcagatc aaggggtgtt
     2941 gttagatgct gaaatgtcca gaaaagaagg acatttagaa tgatatcttg tttgtccttt
     3001 tctgtgggtt tagaacgtgg caggtttata acttcgacac acgcacggtt ctttcttctt
     3061 cacaatccta ttcagaaaca gatttttttt ttcattagag atatgactgt cagttgcagt
     3121 gagttctgca tcccaagtgg agggaattgg gtttgtggca aagagcttga cccaggaaat
     3181 agatggtgcc ccccaaattg tctccacatg aagatgtact gatgacgccc cagaaatgct
     3241 gcttccatat cagctgctgc tagcgccagc gcagactctc agggagtcac cacagcttgt
     3301 cttgtgcttg gtgagtgagg gtctctctac tcagtgtcag acatctacag gaaagaaaca
     3361 actggtggaa aagagcaata aattgcccgg tgctctgcag ggctggaatt tcaaacagaa
     3421 agagggaata agatcctgtg atttttctca cctgcttttc cacgcactgt ggtcatcact
     3481 gtgcaatcta catctagtat gaaatccaca cataggagag ctggggcaca aggggactgg
     3541 aggcagttgc tttgcaagat ggctgaggag aaagcacact gggaacacaa tccagaatgt
     3601 tctaacaata agttttcagt gaataaacca ctggcaagac aattccatgt gcacctttag
     3661 gttacctata tagtctccta ggaagatcag gatgaaagac ctagatgata cccctgagga
     3721 taaaacctcc atcccctaaa atgatttttt ttaaatacca ctgtctttag ctgtccagga
     3781 ggtcagagtg ttttttctgt ctttgggcca agtcctgtct gagacctgta ttttcactct
     3841 tgttaccaaa tctatctccc tagtgcagtg tctccaggcc tgagtttctt ctggaacaga
     3901 ttccatttta gaatggggat tcacaggttc tgtgcatcac cacagtgctc agagaggatt
     3961 ctcctggggt gtcttagagg caggtgccca actcaaatgt attcccaagg tttgctgggc
     4021 tctgggatcc acgagacaac cagagaggga tatctcatga aatttgcatc tggtggctga
     4081 acagtaccta tgttctctgt tttgaatata ctttaatacc tgagagtctt aaaatttgtg
     4141 aacaacgttt ctatagtcct ttattttcaa atgcacattg atcttcactt gctgcatttt
     4201 tactcttcaa ccctgaaact atggtctaca ttaatatgga tttttaaatc acatgtcatt
     4261 acttttgcaa caccatcacc aaaatttttt gctcttttac atttaggttc atctctgtgg
     4321 tctgtgttgt cctgacatgt aaaaagcata tcgtttattg aggttttttt cccccccttt
     4381 tagagcatcc ggaagtgata acacgcaaaa tcacaaagta gcataaatca gtaaattagt
     4441 tgagttgttt ttggggggga ggtgggggta gggggcacag aacaccagaa agagtgttgg
     4501 tgtgtaggta gattccatat taatgaggaa cactgaacta gttggaaatt actgctttct
     4561 ctagaaatat aaagcaaagc actattccaa ggctatggag tagctctaca gcctggcctc
     4621 aactctaaaa gtgtgaagaa tgcaatgggc agagacctac ctgcagtgga ctgtcatttt
     4681 cctttctttc tctgaattac tgctttttct gtgggcatta actatattgc tacagcatct
     4741 agtgtactga gcctgcggtg catggctcag gccttttccc atcgacgtct agggggactc
     4801 tggaccgtgt gaagctaggg ggtgtttctc agcacactgc agaagggcag ctcagaagaa
     4861 tgcagggccc attcagcatg gggatcccag cacatcactg tagaatttga gtgatctatg
     4921 ctgaataaac agtggaatgt gaccagtcaa gtagaaatct tgagtaatca gatggaatgc
     4981 aatctttcta acattaagct accaagatcc tgaatgtcag agatgtactc agagggttaa
     5041 cagacaagca caaggcatgc tgactacatt ggtgtatcca gattgctttg cttttagcca
     5101 gtgctttcta atttttttct cgacattctt gggatagttc aagtttgaaa taattaagtg
     5161 gtggtgttct ttaaggaatt tctataacca aattgatctt atttttgatt tcacttatca
     5221 tagaacaaat atgtatcatt atggcagtgt atctatgtaa ttatcaattt aatcatcacc
     5281 accggtgttt ccatattttt tcccaagtat ttaatatagc tctcttatgg tggtggcctg
     5341 gtgatgggga ccgtctttct tttactgaca catgaccaat catatggtat tttcaaggga
     5401 attttaagat tcatcttttc agtttgatag tagactagtt aaggaagaac tctttcatta
     5461 cttgcatcgt gtaaatcatc tctgtagaca tgtgttcata ttaatgaaca cattttttct
     5521 caacattgta gcagaaatca ttttattcgt catgatcaat gaatatgtga tttgctccag
     5581 atcgttagaa ggaaaagtaa gatttcagtc atcaaaaatg tttttaccgt agccctcatc
     5641 taacttacac gtggtgcata ttaaaataag cagagaaaaa aaaatgtgaa taaactactg
     5701 aaaacacttg gtgttttgtg ttcaatgaga ccttcctgca acctgctccc catgggtggc
     5761 agttaacagg cccatcagat attgttgaaa gaaagcaata tatccatgaa tgaaggctaa
     5821 aattgcaatc ctttaccctt tgaggcatat ttcagttgaa aacaaaaaga aaagaaaatt
     5881 tggcttagag ggtcacagag ctcccatatg accaagtctc aagcacatta aatcatggtt
     5941 gtttactggc caagggcgtc cactagacaa ctctatccct tgcgctgaag ctcaatcgtg
     6001 ctgagggaga gctttcttaa tattactgtg ttgctcttag cccttctctg ggttaggatc
     6061 tgtcagcatt tctatgataa actcctattc tcaaaggttt ttaatttgac cataaaaatg
     6121 tgccccaggc tgaagtttgc tatacagggc tgtaccaaag agtgaaggtt tacttccttc
     6181 tctttccaac ttcttcccca ttctccaagg aaaagaacaa caaaaaaatc tggtatggtc
     6241 cctccttaat agtgatttca gaattttgga aagcaccaag atccaagatg gtagttttaa
     6301 tgtagttact cattcgcaca cattttttaa atttaatggg tcacctggca tatattgtag
     6361 ataacatatc ttttctataa tttgtaagtc aataattttt ttaactgcta catgatattt
     6421 ttttttgccc aaagatttta aaagacttga agttggtcag ttcaaaactc agatttttct
     6481 acacattgtc tgccatgtcc attaggagtt tggggaaaat actctcacac agacccttac
     6541 tttgcatgca gtttagaggg taagatacgt gcttcttttg gggataaaga tttccttact
     6601 taattgtcaa atttcatgga gccattctag tctgttgggg aaaatagtga ttaaaagcac
     6661 ttccaaaatt aacatttttt gacaattcag atatgaaaag aagcagggga aaataataca
     6721 ctttactctt ttcttgctta aaggcaaaca aatcaatgaa acttgaggac acactaaaca
     6781 tttgataact gcaaatgtgc tttaaaaatt ggttcaatgg tgcttacaca tgaaacggta
     6841 acaaatgggg ttcctaggac gtcagaagga atctttagtt tgtatgtaat tacacactag
     6901 aggaggaggt gcttttaagc cagtctttta tttttaatca tctcaaatat gcaaccatac
     6961 atgcagtaac attaagggtc gtaaactggt gggaaacagg aacttcagtg gagaggctta
     7021 aatgcctctg gttagagtgg gggtttttgt ttgtttgttt attgttgggt ttcaacactg
     7081 agcatcattt ctgtgatcaa gtttctaact ggcatgtgtt ttgatcatga ggtttaccat
     7141 atcttgccca tacagacaaa tgagagatct agtttcattt tgttccctaa agaaagaaca
     7201 ctctctaaaa ttaaatcata cctgtaaatt tcttcagcat ttgtttctgt tcaatgaaat
     7261 tgagaccctt aatgttgctt taatgtaaaa ttgaatattt tgtctgtgat atactttaat
     7321 aatttaaagt aagtaatagt tctaaagtct tcactgttgc tactaagaga aaatagaatt
     7381 ttaaaggtga tgataaagat gctataatgt cagttcactc cagtccaatc aaatgtagta
     7441 agaaaaagtc cttgaatagt tctctaggga caatttctca cttgccattg acattaatct
     7501 ttggtgtatt ctcagaaaaa ataaaaagaa attgaaactg gtccaaggtt atagtcatat
     7561 cctcgataac ttttgaaaaa aaattttatt aggaaattaa tactagcctt tttcattctg
     7621 gctgaaagaa aattattaaa ggattagttg agtgtgaaat tcaacagtat tttgctcata
     7681 catactaaaa aggtgcgtag ggacttggcg catttaaaca agtttctgaa aggtttcaat
     7741 ttgactcaag aaaaaaattc aatatttctt ttgaaaatac tgaatttatc acttgctgca
     7801 tggatcagat ggcataggtt aatctttgat tttcagaatc ctaatgaaat aactttcaaa
     7861 caatttgtgt ccttaattaa aggtggaatg agatccaatt tttcccccta atccttcagt
     7921 ttaagctgat acatgtaggt taatgtggaa tgaaatcatc tgtgatatat tatgttcatt
     7981 tatcaactga gcttttttga tgttgcctgt ttttatgtaa aacatgttct taaagttaat
     8041 aaaataatag tacttggtgt atgaactact acataatacc ttagctgaag ggtacgctcc
     8101 tcagttcatt tgccatattg gagtaaaatt tcaccttagt tcaattcaca attgacacat
     8161 tcagttcacg aaggaatagc ccagctgtgt tcttaggggg aattctctct atgcaaggct
     8221 ttaaaaatta gtcatgcggt cagagtgtag ctttcccaca tgtcctccat agcacagtgt
     8281 gtatttcatg agcataattc aaaaacagct atttcaaaaa tgtcccttgt catatattac
     8341 gttcctgcct gggccactga cttggataaa tacaaataaa ccaaatacca ttca
//
